LOCUS       NZ_CP036183             3348 bp    DNA     circular CON 18-JUN-2024
DEFINITION  Escherichia coli strain WCHEC025970 plasmid p4_025970, complete
            sequence.
ACCESSION   NZ_CP036183
VERSION     NZ_CP036183.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN10956393
            Assembly: GCF_004295365.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3348)
  AUTHORS   Long,H., Feng,Y. and Zong,Z.
  TITLE     The complete genome of Escherichia coli strain WCHEC025970
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3348)
  AUTHORS   Long,H., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-FEB-2019) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_004295365.1-RS_2024_06_18
            Annotation Date                   :: 06/18/2024 06:04:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,247
            CDSs (total)                      :: 5,127
            Genes (coding)                    :: 4,888
            CDSs (with protein)               :: 4,888
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 79 of 239
            Pseudo Genes (incomplete)         :: 160 of 239
            Pseudo Genes (internal stop)      :: 53 of 239
            Pseudo Genes (multiple problems)  :: 48 of 239
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP036183.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3348
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="WCHEC025970"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p4_025970"
                     /geo_loc_name="China: Chengdu, Sichuan"
                     /collection_date="2017-10"
     gene            521..665
                     /locus_tag="EYD11_RS27270"
                     /old_locus_tag="EYD11_26365"
                     /pseudo=""
     CDS             521..665
                     /locus_tag="EYD11_RS27270"
                     /old_locus_tag="EYD11_26365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001717510.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            938..1108
                     /locus_tag="EYD11_RS26700"
     CDS             938..1108
                     /locus_tag="EYD11_RS26700"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_152924371.1"
                     /translation="MSYVSERKSELVKAQQRKMDPSSPDSAPINQGLMDFILLAMKVDD
                     GFVQRGRIHGA"
                     /name="hypothetical protein"
     gene            complement(1112..1678)
                     /locus_tag="EYD11_RS26370"
                     /old_locus_tag="EYD11_26370"
     CDS             complement(1112..1678)
                     /locus_tag="EYD11_RS26370"
                     /old_locus_tag="EYD11_26370"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_061583929.1"
                     /translation="MAVFRQCEIREKYLAIYKESFDEVITYLEKAGALEPGVLRLTTID
                     DNAIRAWKSQWKGRSRKHAHGAWDWQNLVSKRARSCKRFDVAVWGEDVLCGLSVGKLTR
                     GKKTVRMDYLEACPTAHPLEKRITMIVVAVALSVAKKVGAQHVAIFNPIKDKEEKVLKH
                     YQSYGFTQRMLYGRFLKNVLYKEVV"
                     /name="hypothetical protein"
     gene            1960..2424
                     /locus_tag="EYD11_RS26375"
                     /old_locus_tag="EYD11_26375"
     CDS             1960..2424
                     /locus_tag="EYD11_RS26375"
                     /old_locus_tag="EYD11_26375"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_072796159.1"
                     /translation="MENKNNLSSEVKNHVSKWGKTNISAGWTIIPNALLENQSRLGLSC
                     IDTMVLINLIMHWWEKDNPPRPSKKRLANMLGVSLKTVQRSFIHLEQCGAIKRIPRYKE
                     GKDNARTTNHYDLNGLVDLLEGFSKELIEEREANRKSEVNRPKKRGNPKS"
                     /name="hypothetical protein"
     gene            complement(3125..3325)
                     /locus_tag="EYD11_RS26385"
                     /old_locus_tag="EYD11_26385"
     CDS             complement(3125..3325)
                     /locus_tag="EYD11_RS26385"
                     /old_locus_tag="EYD11_26385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001558245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin VbhA family protein"
                     /protein_id="WP_061585996.1"
                     /translation="MTDDRTLQLRLTGFRKAEASLRLEGMDPSGTPLYESVKTRILSGD
                     ITFDEGRTEIRAHYQKRANSN"
                     /name="antitoxin VbhA family protein"
     Typing          29..629
                     /name="rep_cluster_2324"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.05"
                     /coverage="92.0"
                     /evalue="0.0"
ORIGIN
        1 cgaataaggc aggcgtaaag ttagctaact ttacatgtcc tgcccggatg ttgccgttgt
       61 atagctgtgc agggaaaagt ggaaaacgac aggaaactgt tgcagccggg tgtgtaatca
      121 gggaggaaac gtgtgtaccg agcgacacga tccctgttca gcattacaga cattacgctt
      181 cctcggtcat tcgctcactt cgttcggtca tgacactgcg gcgagcgtta cctgctcagg
      241 cagaaggcca taaggaggtt ctggggattg ccggttttcc ataggcttcg ccctcctgac
      301 aagcttcaca gaaatcaggt gatttccggc gctcaaatca gaagtggcga aacctgacag
      361 gacttaaaga tccccaccgt ttccgatggg tcgctccctc gtgcgctctc ctgttcccac
      421 cctgaggttt acccgttgca gtccgccgta aggtggcgtt ttctcattcc tacccgtgca
      481 actgggttcc ttgtaggtgg ttcgctccaa gctgggctgt atgcacgaac tccccgttca
      541 gcccgactac cacgtcttta tcaggtaact gtactcttga atccaaccct gaaagacacg
      601 acaaagcgcc actggcggta gccattggta gcagtgagtg cgagaggtgt atcgagagtt
      661 cttgaagtta aagcctgact acagctacac tggaaggaca gatttggtga cacgtctcgc
      721 acaagactgt tacctaggtt caaagagccg caaactcaat gaactggaaa aaaaccgccc
      781 cgcaaaggcg gtttttttct tttgaaagaa aagagtacgc tttgcagaag aggcgagtca
      841 acaacagtca tctctttttg aaaatataga gatctcgtta tttttttatc cacttgttat
      901 agtgtctaca tccccataca agacagagag tcacaagatg agttatgtat ctgagcgcaa
      961 aagtgagctg gtaaaggcac agcaacgcaa aatggatcca tcctctcctg attcagctcc
     1021 aatcaatcag ggtcttatgg acttcatcct gcttgctatg aaagtagatg acggctttgt
     1081 tcaacgtggt cgtattcacg gcgcatagct atcaaacaac ttctttgtac agcacgtttt
     1141 tcaaaaagcg tccatacaac attctttgtg taaacccgta agactggtag tgttttaaca
     1201 ctttttcttc tttgtctttt atcggattga atatggctac gtgctgtgcc cctacttttt
     1261 tagctactga cagagccaca gcaaccacaa tcattgtgat tcgtttctcc aacggatgag
     1321 ctgttggaca agcctctaga taatccattc taactgtctt tttaccacgg gttagctttc
     1381 caactgaaag cccacaaagc acatcctctc cccaaacagc cacatcaaat cttttacaag
     1441 aacgagctct ctttgataca aggttctgcc aatcccaagc accatgagcg tgcttacgag
     1501 acctgccttt ccattggctc ttccatgcac gaatagcatt gtcatcgatt gtagtgagcc
     1561 ttaaaacgcc gggctctaaa gcacctgcct tctcaagata agttataact tcatcgaatg
     1621 actctttgta aatcgcgaga tatttttctc taatttcgca ttgtctaaaa accgccacgc
     1681 tcgtctccct cctgaccttg accagtaaac attttgccat aaaaaaacca ctttaaatca
     1741 tttgctaaca ttttggttag tggtcaacaa agaccatatc ctatggtaca tttaaacccc
     1801 accctgtgga ctttaaaaac catagggtat ggtctgattt gaccacacag aaaaagatat
     1861 agtgggacaa tacgttaaga caatgacgca aggtgaacgg taaaacgttc aatatataaa
     1921 atgccctcta gcctcagatg accacaggat gcatgtttta tggaaaacaa aaataacctg
     1981 tcgagcgaag taaagaacca cgtaagcaag tgggggaaga caaacatttc tgcaggatgg
     2041 acgattatcc caaatgcctt actggaaaat cagtccaggt tgggtcttag ctgtattgat
     2101 acgatggttc tcataaacct cataatgcat tggtgggaaa aagataaccc cccaagacct
     2161 tcgaaaaaga gactggcgaa catgctgggc gtttcactaa aaaccgttca aagaagtttt
     2221 attcatttgg aacagtgtgg tgccatcaag cggatacctc gatacaaaga agggaaagac
     2281 aatgccagaa caacaaacca ttacgatctg aacggactgg tagacctgct ggaaggtttt
     2341 tcgaaagagc ttatcgaaga aagagaggcg aatcgaaaaa gtgaagttaa tagacctaag
     2401 aagcgcggta atccgaaaag ttaataggct gacaaacaca ctctcctgta gagagtccaa
     2461 tttaccataa catctcttgc gtgcaccgcc gcagaggccc tcagcgcgtt tctgccgcgt
     2521 gcgggccgtt tccggcacct ttccctgtcc atatcgtgaa aaaacgcgcc tgatgatttc
     2581 tgcgcgcctg catggctatg cacggaagtg aatggcggct ggtttcggac gaatgctggc
     2641 actaaaggcg aattttgaac aaaaaaaacg gacatcgagg gtcaggatgt ccgacaaaat
     2701 ttgcacaaac agtcagccgg taccgatcag agagccggca aataagtatg acagtaaaaa
     2761 gtggttatca actgcccccg cgctgctgat tgcgcagttt tagggctttg gccagtgcag
     2821 gcaggcccgg ccggaatcag gggatctggt aacggtgaac gggtgcacag ttgactgtgc
     2881 ggccgtgagg cgttggccaa gccgccaggc gcggcagctt ccggtgggta caaacgccag
     2941 cactttgcga atcctgccct agatctcaac agcagagaaa tttcgtacgc acgaaactca
     3001 gcgtatcggg cggcgcctga tgaaacattc tggagtgtcg gcggtactcc aaaaacacat
     3061 cttgtcccgc tgatgttgtc actggcgcaa agcggcggta atgacaagac atttcctgtc
     3121 cgcgtcagtt actgttcgcc cgcttctggt aatgagcccg tatctcagtg cggccttcat
     3181 cgaaggtgat gtcaccggaa agaatccttg tctttaccga ttcataaagc ggggttcctg
     3241 atggatccat cccttcaagc ctgagagagg cctccgcttt acggaaccct gtcaggcgca
     3301 gttgcagtgt ccgatcatca gtcatggttt ttcctttgag cagtcatg
//
