LOCUS       NZ_CP035942             2123 bp    DNA     circular CON 22-APR-2024
DEFINITION  Acinetobacter cumulans strain WCHAc060092 plasmid p7_060092,
            complete sequence.
ACCESSION   NZ_CP035942
VERSION     NZ_CP035942.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN08772519
            Assembly: GCF_003024525.3
KEYWORDS    RefSeq.
SOURCE      Acinetobacter cumulans
  ORGANISM  Acinetobacter cumulans
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Qin,J., Maixnerova,M., Nemec,M., Feng,Y., Zhang,X., Nemec,A. and
            Zong,Z.
  TITLE     Acinetobacter cumulans sp. nov., isolated from hospital sewage and
            capable of acquisition of multiple antibiotic resistance genes
  JOURNAL   Syst Appl Microbiol 42 (3), 319-325 (2019)
   PUBMED   30808586
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Qin,J., Long,H., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-FEB-2019) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
REFERENCE   3  (bases 1 to 2123)
  AUTHORS   Qin,J., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAR-2018) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003024525.3-RS_2024_04_22
            Annotation Date                   :: 04/22/2024 04:03:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,515
            CDSs (total)                      :: 3,393
            Genes (coding)                    :: 3,310
            CDSs (with protein)               :: 3,310
            Genes (RNA)                       :: 122
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 96
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 26 of 83
            Pseudo Genes (incomplete)         :: 44 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 11 of 83
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP035942.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Acinetobacter cumulans"
                     /mol_type="genomic DNA"
                     /strain="WCHAc060092"
                     /isolation_source="Sewage"
                     /type_material="type strain of Acinetobacter cumulans"
                     /db_xref="taxon:2136182"
                     /plasmid="p7_060092"
                     /geo_loc_name="China: Chengdu, Sichuan"
                     /collection_date="2018"
     gene            complement(632..847)
                     /locus_tag="C9E88_RS17475"
                     /old_locus_tag="C9E88_016735"
     CDS             complement(632..847)
                     /locus_tag="C9E88_RS17475"
                     /old_locus_tag="C9E88_016735"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin VbhA family protein"
                     /protein_id="WP_066244013.1"
                     /translation="MNQAEKELRQQQFDGFKANYEYYDLTPSKEKIQAFERYVSGEIDA
                     EQLQRIFHNLSGSTENFIMLPMDWFC"
                     /name="antitoxin VbhA family protein"
     gene            complement(858..1835)
                     /gene="repM"
                     /locus_tag="C9E88_RS17480"
                     /old_locus_tag="C9E88_016740"
     CDS             complement(858..1835)
                     /gene="repM"
                     /locus_tag="C9E88_RS17480"
                     /old_locus_tag="C9E88_016740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846161.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_066244015.1"
                     /translation="MSKLVVKDNALMNASYNLDLVEQRLILLAIVEARESGKGINANDP
                     LTVHAESYINQFGVARQTAYQALKDACDDLFARQFSYQEINERGNTENVRSRWVSEVRY
                     VDAEATVKLIFAPIVIPLITRLEERFTQYEMKQISELSTGYAIRLYELLICWRTTGKTP
                     IIELADFRQKMGVLDAEYQRMYDLKKYVLEPSLKQINEHTDITASYEQHKKGRTITGFS
                     FKFKQKKKTGTETPKNSDFSDSKQKPVEIPDEVVKQPKNANMDVLEHRKSKITGLIMSN
                     GLADRFKRGDESVMDMMKRIKEEITTDATADQWQSKLEEFGVVF"
                     /name="repM"
ORIGIN
        1 gtcaggggca atttttgcaa atgacggaga aaaaccaaag attttaaaag cattgagcaa
       61 ggacacacag agggtacttt ttcactaccg ttcaaaattc ctactctgtg ccttgttggg
      121 gcaagcccca aaccccaaaa agcaaaagca aaggcaaaaa tggcactcgc ttcgctcgtg
      181 tcttttcaaa agcaaaagca cacgatagtt aaagttttca tgaaatcaga tgctaaaaaa
      241 gcagtttttc agagggggga ataatcgaac tttcattgtg tcgctcgtag acactcgcta
      301 ataactttct ttgaaaaacc aaaaaaaaga caaagaaaat tttctttgtc tttttacttt
      361 ttcgatagaa aaacgcactc aaacgatagt ttgggaaagt gcgccctttg ggatttgtca
      421 tacaaaataa aaattcgtta aacgatttcc tatcctgcca taggcttatt tttattttta
      481 cttctgatct tttagtcatg actaaaaaac tttgtcatac aaaatcacaa aacgccatgc
      541 gttttcctat cctgccttag ctcccttagc tcccttagct cccttagctc aaactcaata
      601 taaaattatt agtcatgact aaacggctta atcagcaaaa ccaatccata ggtaacatta
      661 taaaattttc agttgaaccg cttaaattat ggaaaattct ttgcaactgt tcagcatcaa
      721 tttcacctga aacataacgt tcaaacgcct gtattttttc ttttgatggc gttaaatcgt
      781 aatattcata attggctttg aaaccatcaa attgctgttg tcttaattct ttttcagctt
      841 gattcattgc tcaactccta aaaaacaacg ccaaattctt ctaattttga ttgccattgg
      901 tcggctgttg catcagttgt aatttcttct ttgatgcgtt tcatcatatc catgactgac
      961 tcatcaccac gtttaaaccg atctgctaag ccattcgaca tgattaaccc tgtaattttg
     1021 cttttacggt gttctaaaac gtccatattt gcgtttttag gctgttttac gacttcatca
     1081 gggatttcta cgggtttttg cttagaatcg ctaaaatcgc tgttttttgg cgtttctgtc
     1141 cctgtttttt tcttctgttt aaacttgaat gaaaatcctg tgattgtccg cccttttttg
     1201 tgctgttcgt agctcgctgt gatgtctgta tgctcgttga tttgtttgag agacggctct
     1261 aaaacgtatt tttttaaatc atacatgcgt tgatattcag catcgagaac acccattttt
     1321 tgccgaaagt cagccaattc tatgattggt gttttccctg tggttctcca gcagatcagt
     1381 aactcataca aacgaattgc ataacctgtt gaaagctcgc ttatctgttt catttcatac
     1441 tgagtaaaac gttcctcaag ccttgtaatt aaagggatga cgataggagc gaagattaac
     1501 ttcactgtag cttctgcatc cacatagcga acttcactaa cccaacgtga acggacgttt
     1561 tcggtatttc ctcgttcatt gatttcctgg taactgaatt gacgtgcaaa tagatcatca
     1621 caagcatctt tgagggcttg gtaggctgtt tgtctagcta cgccaaattg attgatataa
     1681 ctctctgcat ggacggttaa agggtcattg gcattaattc cctttcctga ctctcttgct
     1741 tcaacaatcg ccaacaagat taagcgctgt tcaacgagat ccaagttgta actagcgttc
     1801 attagggcat tgtctttcac tactaattta ctcataagac gtgaattaaa attttttcgt
     1861 taaaacgaat ttatcataac atctcgtctt atggtaggta aatctcgtct tattggtagg
     1921 taaatctcgt cttattggta ggtaaatctc gtcttattgg taggtaaatc tcgtcttatg
     1981 tcctctgtat gccttgctat acttgcattt cagtatggct aaaagcattt aaaagcattt
     2041 aaaaacatta aaagcccaat ttttcatgaa aattgccctc ggttttcgct aaagctcaaa
     2101 cttttttgac ttcacttcgt tac
//
