LOCUS       NZ_CP035941             4692 bp    DNA     circular CON 22-APR-2024
DEFINITION  Acinetobacter cumulans strain WCHAc060092 plasmid p6_060092,
            complete sequence.
ACCESSION   NZ_CP035941
VERSION     NZ_CP035941.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN08772519
            Assembly: GCF_003024525.3
KEYWORDS    RefSeq.
SOURCE      Acinetobacter cumulans
  ORGANISM  Acinetobacter cumulans
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter.
REFERENCE   1  (bases 1 to 4692)
  AUTHORS   Qin,J., Maixnerova,M., Nemec,M., Feng,Y., Zhang,X., Nemec,A. and
            Zong,Z.
  TITLE     Acinetobacter cumulans sp. nov., isolated from hospital sewage and
            capable of acquisition of multiple antibiotic resistance genes
  JOURNAL   Syst Appl Microbiol 42 (3), 319-325 (2019)
   PUBMED   30808586
REFERENCE   2  (bases 1 to 4692)
  AUTHORS   Qin,J., Long,H., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-FEB-2019) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
REFERENCE   3  (bases 1 to 4692)
  AUTHORS   Qin,J., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAR-2018) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003024525.3-RS_2024_04_22
            Annotation Date                   :: 04/22/2024 04:03:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,515
            CDSs (total)                      :: 3,393
            Genes (coding)                    :: 3,310
            CDSs (with protein)               :: 3,310
            Genes (RNA)                       :: 122
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 96
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 26 of 83
            Pseudo Genes (incomplete)         :: 44 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 11 of 83
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP035941.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4692
                     /organism="Acinetobacter cumulans"
                     /mol_type="genomic DNA"
                     /strain="WCHAc060092"
                     /isolation_source="Sewage"
                     /type_material="type strain of Acinetobacter cumulans"
                     /db_xref="taxon:2136182"
                     /plasmid="p6_060092"
                     /geo_loc_name="China: Chengdu, Sichuan"
                     /collection_date="2018"
     gene            join(4444..4692,1..27)
                     /locus_tag="C9E88_RS17470"
                     /old_locus_tag="C9E88_016730"
     CDS             join(4444..4692,1..27)
                     /locus_tag="C9E88_RS17470"
                     /old_locus_tag="C9E88_016730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007159983.1"
                     /GO_process="GO:0008219 - cell death [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system prevent-host-death
                     family antitoxin"
                     /protein_id="WP_106986749.1"
                     /translation="MQILNFSQARACFKEVMEDVCRDHEPTVITRQRGNPVVVMSLDDF
                     NGMKETIYLLSSKKNANRLLESVARIKAGQTTKRNLIENDCQTEAT"
                     /name="type II toxin-antitoxin system prevent-host-death
                     family antitoxin"
     gene            2..310
                     /locus_tag="C9E88_RS17445"
                     /old_locus_tag="C9E88_016705"
     CDS             2..310
                     /locus_tag="C9E88_RS17445"
                     /old_locus_tag="C9E88_016705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019751770.1"
                     /GO_process="GO:0008219 - cell death [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="WP_106986750.1"
                     /translation="MTAKQKQRNKKQAQENVALSWDDHAWDDYQYWLKTSPEIFSKVND
                     LINESLKTPFSGTGKPEPLKENLSGFISRRITKEHRLVYMYEKGVLHIASCRYHYDD"
                     /name="Txe/YoeB family addiction module toxin"
     gene            complement(404..592)
                     /locus_tag="C9E88_RS17450"
                     /old_locus_tag="C9E88_016710"
     CDS             complement(404..592)
                     /locus_tag="C9E88_RS17450"
                     /old_locus_tag="C9E88_016710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010591710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_106986751.1"
                     /translation="MVEKEITKFEKEILLQEKIEQLENELKEFSDLQKKVYSDRLQKSI
                     VGLENRIQRIKKMLYTT"
                     /name="hypothetical protein"
     gene            complement(592..1509)
                     /gene="repM"
                     /locus_tag="C9E88_RS17455"
                     /old_locus_tag="C9E88_016715"
     CDS             complement(592..1509)
                     /gene="repM"
                     /locus_tag="C9E88_RS17455"
                     /old_locus_tag="C9E88_016715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871874.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_106986752.1"
                     /translation="MLIVKDNALINASYNLELVEQRLILLAIIEARENGKGINANDPLT
                     VHASTYIQTFNVEKHSAYTSLKEACKSLFARQFSYEEINKNGNLTQYTSRWVSKIGYTK
                     NEATVEIIFAPDVVPLITRLEEHFTSYELEQVAQLQSKYATRLYEILIAWRSTGKTPVL
                     TINEFRKQIGVIDSEYKIISNFKLRVLEPSIKQINDLTDIDVTYEQHKKGRTITGFSFN
                     FKQKQQAKKIETNIDPDTADLFKKMTDAQRHMFSHKLSEMPEMSRYSQGTESYQQFAIR
                     IAEMLLQPEKFRELYPLLEKAGFK"
                     /name="repM"
     gene            2193..2702
                     /locus_tag="C9E88_RS17460"
                     /old_locus_tag="C9E88_016720"
     CDS             2193..2702
                     /locus_tag="C9E88_RS17460"
                     /old_locus_tag="C9E88_016720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005200081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_106986753.1"
                     /translation="MKNQCVVVLTARGKSRILKEGGSQAWRLNAHHAGKCSYVVCVQNR
                     NKSWGQPEAKHHTAFLIGKISSISQSQEEGSQGRWIINISEYADISIPNVWDGNRNPVA
                     YRTLEELNIDLENLQFNKLEQEPNVLEQANKPKEELEKISIPEAKRLLANYFDVPQENI
                     SITINM"
                     /name="hypothetical protein"
     gene            complement(2888..4021)
                     /gene="mobV"
                     /locus_tag="C9E88_RS17465"
                     /old_locus_tag="C9E88_016725"
     CDS             complement(2888..4021)
                     /gene="mobV"
                     /locus_tag="C9E88_RS17465"
                     /old_locus_tag="C9E88_016725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005080191.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_106986748.1"
                     /translation="MSFAILRIQKLKSFADVGGSLSHNYRNRETLNADDARTHLNEHEL
                     DTNEKCMTAIRDRIPEKRRKDAVLCIEHLITASPEWDGWGTEKETAFFEQSKKWLENKY
                     GKNNVVSTTIHRDETTPHLVAYVVPVDEETGRLNAKKYIGGSRHTLSQMQTDFAVEVKD
                     LGLDRGVQGSKAKHTSIQEYYEKLNNYENEPGIEKGLTYEVPEPEFFESKNAYGERVAK
                     AMIDQIFDQIGPRFDNANLLASQSKKLKKELSDARRTLAEVQQRAKPYLDIINKYNNPN
                     LTKQFDEQIFKLEQNFDRQLEHHIFLNEQREQERFNQQRELREQLRIEQEQKKQLAEQE
                     RQEKERLAFLRRQELEKQRKNEPKKPDNSNDNDYSPS"
                     /name="mobV"
     Typing          complement(2892..4051)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.742"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aatgactgcc aaacagaagc aacgtaacaa gaaacaagcg caagaaaatg tagctctaag
       61 ctgggatgac catgcttggg atgactacca atattggttg aaaacctctc ctgaaatttt
      121 tagcaaagta aatgatctga ttaacgaatc attgaaaacg ccattttctg gcacagggaa
      181 acctgaacct ctaaaagaaa atctctccgg ttttatttct cgacgtatca cgaaagaaca
      241 ccgtcttgta tatatgtatg agaagggtgt tttgcatatt gcttcatgca gataccacta
      301 cgatgactaa tgtctcgtaa tatttaagac tatataaatt gaaaggcgtg gatactgatc
      361 tgcgcctttt ttattagaaa agctgtaaaa ccctgacaac tttttaggtt gtataaagca
      421 tttttttgat tcgttgaatt ctattttcta aacccacgat acttttttga agtcgatcgc
      481 tatacacttt tttttgaaga tcagaaaatt ctttaagttc attttcaagc tgctcaattt
      541 tttcttggag caaaatttct ttctcaaatt ttgttatttc tttttcgacc attacttaaa
      601 tcctgctttt tctaaaagag gatataactc tctaaatttc tcaggttgta aaagcatttc
      661 agcaatacga atggcaaatt gttgatagct ctctgtacct tgggaatatc tcgacatttc
      721 gggcatctca gacagtttgt gagaaaacat atgtcgttgt gcatctgtca tttttttgaa
      781 tagatcggct gtgtccggat ctatattagt ttctattttc tttgcttgtt gcttctgctt
      841 aaagttgaat gaaaagcctg tgattgtccg ccctttttta tgctgttcat acgtcacgtc
      901 aatatctgtt aaatcattga tctgcttaat tgatggctct aatacacgta acttaaaatt
      961 ggaaataatt ttatattcag aatcaataac acctatttgc tttctaaact cattaatagt
     1021 tagtactggg gttttaccag tgctgcgcca cgcaattaat atttcatata gccgtgtagc
     1081 atacttactt tgaagctgtg caacttgttc aagctcataa cttgtgaaat gttcttcaag
     1141 gcgtgtaatt aaaggaacaa catcaggcgc aaatataatt tctacagttg cttcattttt
     1201 tgtatagccg atttttgata cccatcggct tgtatactgt gtcaaatttc catttttatt
     1261 tatttcctca taactaaatt gtcttgcaaa taaactctta catgcttctt ttaagcttgt
     1321 ataagcacta tgtttctcaa cattaaatgt ttgtatgtat gtgcttgcat gtactgttaa
     1381 tgggtcgttt gcattaatac cctttccatt ttctcttgct tcaataatcg ctaatagaat
     1441 taatcgttgt tccacaagct ctaagttgta actggcatta attaaggcgt tatcttttac
     1501 aatgagcata tttttttcct gttctacatt acggctcaca atcaaatcct ccttacgaca
     1561 aaaccaatat acacaaatta aagcgtaagt aaatatacat aaaataatat gtatagataa
     1621 ggtctataga tgtatagata tgggtctata gatgtataga tatgggtcta tagatgtata
     1681 gatatgggtc tatagatgta tagatatgaa gctcaaaagc cttgctatca cagtgtttca
     1741 gagagcctaa aagcatttaa aagcatttaa aaacattaaa agcccagaaa aagtcaaaat
     1801 caaaggctat ggcactcgct tcgctcgtgt cgtttcaaga gcaaaagcaa agtcaaaagc
     1861 actaataatg cattcgctat gctcatgctt ttttaagagc aaaagcatca atacttcact
     1921 cgtagatatt cgctccgctc tgttaatctc gtttaatgtt gaaatggaaa ctttgtaatc
     1981 agagaaaaaa caagggcgaa attttaatgt tgctcaatcg ctcgtagaca ctcgctcggg
     2041 tacttaaaca tgaggttcta tactccaaga gcaaaaaaag ctaaatttca gagtgaatag
     2101 tacctcattg tactatgcaa ataaaaccga atgtactaat attagtacat gcagttttat
     2161 ttgcattaaa tttttgtact aagggtgttt aaatgaagaa tcagtgtgtt gtagttttaa
     2221 cagctcgagg aaaatctaga attttaaaag aaggaggaag tcaggcttgg agattaaacg
     2281 ctcaccatgc aggaaagtgc agttacgtgg tttgcgttca aaaccgaaat aaatcttggg
     2341 gtcaacctga agcaaagcac cacacagcct ttttaatcgg taaaatatct agtatcagcc
     2401 aatctcaaga agaaggctct caaggtcgtt ggataattaa tatttctgaa tatgcagata
     2461 tttctattcc taatgtatgg gatggaaata gaaacccagt tgcttataga actttagaag
     2521 agctaaacat tgacttagaa aatttacagt tcaataagtt agagcaagaa cctaatgttt
     2581 tagagcaagc gaataaacca aaagaagaat tagagaaaat atcaattcct gaagctaaaa
     2641 gattgttagc aaactatttt gatgttcctc aagaaaatat ttctattact attaatatgt
     2701 aatttcaaag gcaaaagaga actatctttt gccttttctt taagcgaaat ttcattgtga
     2761 tgctcgtaaa cactcgctaa aaatctttca tttgcgttct aatgaatttt atttatttca
     2821 actatacttt tatgcatgaa acccaaaaca tggctaaaac ccccgttttt ttgcgtttaa
     2881 atggatgtta tgagggggag tagtcgttat cgttgctgtt atcaggtttt ttgggttcat
     2941 tttttctctg tttttccaat tcttgacgtc ttaaaaatgc caaacgctct ttttcctgtc
     3001 tttcttgctc agccagttgt tttttctgtt cctgttctat gcgtaattgc tctctaagtt
     3061 ctcgttgttg attgaatctt tcttgttctc gttgttcatt tagaaatata tgatgttcta
     3121 gttgcctatc aaaattctgc tctaatttaa aaatttgttc atcaaattgt ttagtaagat
     3181 tcggattatt gtatttgttg attatatcca aatacggttt tgctctttgc tgtacttcag
     3241 ctagtgtcct tcttgcatct gacagttctt tttttaactt tttcgattga ctggccagca
     3301 agttagcatt gtcaaatcgc ggtccaattt gatcaaatat ctgatcaatc atagcctttg
     3361 caactctctc accgtaggca tttttagatt caaaaaactc aggttcaggc acttcatagg
     3421 tgagtccttt ttcaatgcct ggttcattct cgtaattgtt caatttttca taatattctt
     3481 gaatagacgt atgttttgct ttgctcccct gtacgccccg atctaatcct aaatctttta
     3541 cttcaactgc aaaatcagtc tgcatctgtg aaagtgtatg tcgagatcca ccgatatatt
     3601 ttttagcatt caatcgtcct gtttcttcgt ccactggaac aacgtaggcg acaaggtgag
     3661 gagttgtttc atcacggtga atggtggtgc tgacgacatt atttttaccg tatttatttt
     3721 cgagccattt ttttgattgc tcaaaaaatg cagtttcttt ttcagttccc cagccatccc
     3781 actccggact cgctgtaatc aaatgctcaa tacacaaaac tgcatcttta cgtctttttt
     3841 caggaatacg atcacgaata gcggtcatac atttttcgtt tgtatctagc tcatgctcat
     3901 tcaaatgtgt acgagcatca tcggcattta acgtttctcg attgcgataa ttatgcgaaa
     3961 gactgcctcc aacatcggca aatgacttga gtttctgaat acgcaaaata gcaaatgaca
     4021 ttggagaaat ccgacaccgc aaaaagcaca cgatagcaga ggtttttgag ctttgcgata
     4081 gtgaagtgga ctcactccac tttcaaataa cctaaaggta tattgaaagt tcatgagtaa
     4141 ggggggattc cccccttaac ccccattaaa aagactatct tgaaacaaaa cattcaactc
     4201 atttttgatt ttatgttttg gtgattaatt ttttattgaa aaaagattgt accaatactt
     4261 gtacaatctt tttttgtgat gtataatcca tttcatatct tgatcggctc tacatgggct
     4321 gatgaccacc tcgcacaaaa tggtgtgcac atatggtttt aacaatttat ccctagaaga
     4381 acatctagaa cgatttttag ttttaacaac ttatcatccc tgtttatagg gaaggaaaac
     4441 aaaatgcaaa tacttaactt tagccaagct cgggcttgct ttaaggaggt aatggaagac
     4501 gtttgtcgag atcacgaacc aactgtgata actagacagc gaggtaatcc tgtagttgta
     4561 atgtcattgg atgattttaa cggtatgaag gaaacgatat acttgttgag ctcaaagaag
     4621 aatgcaaaca gattattaga atccgtcgct cgaattaagg caggtcagac cacaaaaagg
     4681 aatctgatag aa
//
