LOCUS       NZ_CP035939             9699 bp    DNA     circular CON 22-APR-2024
DEFINITION  Acinetobacter cumulans strain WCHAc060092 plasmid p4_060092,
            complete sequence.
ACCESSION   NZ_CP035939
VERSION     NZ_CP035939.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN08772519
            Assembly: GCF_003024525.3
KEYWORDS    RefSeq.
SOURCE      Acinetobacter cumulans
  ORGANISM  Acinetobacter cumulans
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter.
REFERENCE   1  (bases 1 to 9699)
  AUTHORS   Qin,J., Maixnerova,M., Nemec,M., Feng,Y., Zhang,X., Nemec,A. and
            Zong,Z.
  TITLE     Acinetobacter cumulans sp. nov., isolated from hospital sewage and
            capable of acquisition of multiple antibiotic resistance genes
  JOURNAL   Syst Appl Microbiol 42 (3), 319-325 (2019)
   PUBMED   30808586
REFERENCE   2  (bases 1 to 9699)
  AUTHORS   Qin,J., Long,H., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2019) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
  REMARK    Sequence update by submitter
REFERENCE   3  (bases 1 to 9699)
  AUTHORS   Qin,J., Long,H., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-FEB-2019) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
REFERENCE   4  (bases 1 to 9699)
  AUTHORS   Qin,J., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAR-2018) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003024525.3-RS_2024_04_22
            Annotation Date                   :: 04/22/2024 04:03:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,515
            CDSs (total)                      :: 3,393
            Genes (coding)                    :: 3,310
            CDSs (with protein)               :: 3,310
            Genes (RNA)                       :: 122
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 96
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 26 of 83
            Pseudo Genes (incomplete)         :: 44 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 11 of 83
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP035939.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9699
                     /organism="Acinetobacter cumulans"
                     /mol_type="genomic DNA"
                     /strain="WCHAc060092"
                     /isolation_source="Sewage"
                     /type_material="type strain of Acinetobacter cumulans"
                     /db_xref="taxon:2136182"
                     /plasmid="p4_060092"
                     /geo_loc_name="China: Chengdu, Sichuan"
                     /collection_date="2018"
     gene            343..1521
                     /locus_tag="C9E88_RS17370"
                     /old_locus_tag="C9E88_016630"
     CDS             343..1521
                     /locus_tag="C9E88_RS17370"
                     /old_locus_tag="C9E88_016630"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_162368857.1"
                     /translation="MLSSLQIKNFRSLKNVEIPKLGLINLIVGNNNSGKSSLIESLLIL
                     ANNADEEILNTLAFSHGEPTLSDREEEEFTSPFQPFESFFPNRQFPREDNVKIVVGELD
                     SSNFLTIEHAFEKEFLIKFEDEEGNILRRLKRDVISKLEIDEMLEDPEQNLQINSILKI
                     ENKGSVSRISLEPDRRRATTISRSLDSKLNIPYSYIPTSFLDPDELALDWDKLVLTPYQ
                     DYIIEALQIIEPNLENISFIKSVNYRRSRYRSSQRTPIVKLKDHSLPFPLSSMGDGILR
                     ILQLVLKLHSARGGILLIDEFDNGLHHSVQEKVWKLVFSLAESLKIQVFATTHSSDCVK
                     AFSKVSKERTDIEGILIQIGKSTRKSNFGEVIANILDENQLATFIKSDFEVR"
                     /name="ATP-binding protein"
     gene            1518..2180
                     /locus_tag="C9E88_RS17375"
                     /old_locus_tag="C9E88_016635"
     CDS             1518..2180
                     /locus_tag="C9E88_RS17375"
                     /old_locus_tag="C9E88_016635"
                     /inference="COORDINATES: protein motif:HMM:NF022971.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3226 domain-containing protein"
                     /protein_id="WP_106986765.1"
                     /translation="MKHLIVEGLGDKVFFEKYCQYYSYNVDVNILTPEDINPQYYTTKN
                     GVISSSLDDLISQITLGIIKNLGIVIDADYIKDKSGIEKTKESLTQKLAPHGYMLVETD
                     EGIIAKHNDGLIDIGIWIMPNNDEDGNIENWIAELISESETPLYEKAKLSVSELAEEGL
                     QKFKPSRVQKAQIATWFAWQKSPGHGLDFFFNAPLIDMNHIKYTNFCTWFRRTFEIQ"
                     /name="DUF3226 domain-containing protein"
     gene            complement(2787..3428)
                     /locus_tag="C9E88_RS17380"
                     /old_locus_tag="C9E88_016640"
     CDS             complement(2787..3428)
                     /locus_tag="C9E88_RS17380"
                     /old_locus_tag="C9E88_016640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016927662.1"
                     /GO_process="GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="WP_004787586.1"
                     /translation="MSKKDIAPQRLTREIVLRTALDMLNEEGIDSITTRKLAQRLGIKS
                     PTLYWHFKNKSLLMEAMAETIINEHHLVSLPIDGMTWQDWLLANSVSFRRALLAYRDGA
                     RLHARTSPSQGHFNTIEAQVALLSHAGFSPVEAVALLMTLGRFIVGWVLEEQQEEIRSD
                     PPFEADPTIYPLMLQGVNTLQNMNADDIFENGIRMVIIGAERQLDIKMQT"
                     /name="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
     ARG             3561..4681
                     /name="tet(39)"
                     /gene="tet(39)"
                     /gene_name="major facilitator superfamily (MFS) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="tetracycline antibiotic"
                     /identity="100.0"
                     /coverage="94.43"
     ARG             3495..4678
                     /name="tet(39)"
                     /gene="tet(39)"
                     /gene_name="tetracycline efflux MFS transporter Tet(39)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(4911..5474)
                     /locus_tag="C9E88_RS17390"
                     /old_locus_tag="C9E88_016650"
     CDS             complement(4911..5474)
                     /locus_tag="C9E88_RS17390"
                     /old_locus_tag="C9E88_016650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018680126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_227866004.1"
                     /translation="MNTTKFSVMDASKAFKKSRTTIYEAIKNGELSRDHDGLIDLSELI
                     RVYGNPIGVQSNTRTEHVQKDVQVRVQSEIENVLKDQISLLKNQLDLANHREKALMQHI
                     EDLTHRIEFKGQLEQPKTEVEEHKVAPPVHSEMTENPKLEPDQKDDGLTTPVETENKRI
                     PVPEHVEQEPEKRGFWSRFFRPYD"
                     /name="plasmid replication DNA-binding protein"
     gene            complement(5535..6452)
                     /gene="repM"
                     /locus_tag="C9E88_RS17395"
                     /old_locus_tag="C9E88_016655"
     CDS             complement(5535..6452)
                     /gene="repM"
                     /locus_tag="C9E88_RS17395"
                     /old_locus_tag="C9E88_016655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005021644.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_106986761.1"
                     /translation="MRDLVVKDNALINASYNLDLVEQRLILLAIVEARGSGRGINANDP
                     LEVHAESYVNQFNVARQTAYQALKDACKDLFARQFSYQEVNKRGNIENVLSRWVSEIRY
                     IDDEATVKLIFAPAIVPLITRLEEQFTKYELQQISDLSSAYAVRLYELLIAWRSTGQTP
                     VIELAEFRQKIGVLDDEYTRMGNFKDRVLNLAIAQVNEHTDINVKCEQHKKGRNISGFS
                     FTFKQKKVITSKSSTSLELFSKMTDAQRHRFANKLSELPDMNKYSQGTESYPQFAVRIA
                     EMLKDKEKFEELLPHLKKVGFSTK"
                     /name="repM"
     gene            7660..8046
                     /locus_tag="C9E88_RS17400"
                     /old_locus_tag="C9E88_016660"
     CDS             7660..8046
                     /locus_tag="C9E88_RS17400"
                     /old_locus_tag="C9E88_016660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005200047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_106986762.1"
                     /translation="MNSTRTVAVRLDNQVAQEYERLAQLKGDKLGTYLRDLLTSNLQMA
                     SIERQASRIETLVNDFEKQINYSLDKFTEANYLNKKFFEDFGGVYMMLLSTLMHLKVER
                     DDIRGMQRQGNAYAQRNYFEGKKP"
                     /name="hypothetical protein"
     gene            8043..8213
                     /locus_tag="C9E88_RS17655"
     CDS             8043..8213
                     /locus_tag="C9E88_RS17655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005200045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_167514225.1"
                     /translation="MSNIIQLSKPCAFCDSRDNVQLFAGLMLCGNCQENIRITNPGMFE
                     ANNQIEQKAQD"
                     /name="hypothetical protein"
     gene            complement(8273..9607)
                     /locus_tag="C9E88_RS17405"
                     /old_locus_tag="C9E88_016665"
     CDS             complement(8273..9607)
                     /locus_tag="C9E88_RS17405"
                     /old_locus_tag="C9E88_016665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016658832.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="WP_137287915.1"
                     /translation="MLITMSDKEIQRLAVLQDVRDHRITQVRAAEILNLSTRQITRLLQ
                     KLNQDGVSGMAHASRGQPGHRRHDDLLKSKCLSIIAEHLLGFGPTLAHEKLSSMFDLNI
                     PVETLRRWMTANDLWIPRSKRLKRPYQPRYNRDCFGELIQIDGSYHDWFEGRAAKCCLL
                     VYIDDATGKLLHLRFCEAETTFDYMLSTRAYIEQYGKPLAFYSDKHSVFRVNQKSSQDS
                     QITQFGRILNELNIDIIFANSPQAKGRVERANRTLQDRLIKEMRLEGIGSIAEANIWLP
                     CFIEQFNQKFAKCARNSKNLHRPLSESDLELDDIFTWQEPRKVTKNLTLTYDKCIYMLE
                     PTELNHKLVGLYISFLEYPDGTVAIMHEGRKINYSVFNKLAGLQQNAVVENKRLGSVLA
                     HIQQQHEELEKQNKRSRLKKSMPSRKAQKAVIEQRYLNPVLDSCS"
                     /name="ISNCY family transposase"
     Typing          complement(5765..6452)
                     /name="rep_cluster_2314"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.565"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 cctgggctac gtataaggtg tattatgtta attttagaag aacttaagca tttagtattg
       61 tatttcactc cccatcataa atttttcaag aattattccc ttatggaaaa atagtagtaa
      121 tgatgatata aattgttaaa attcaaatat tagtgactaa ataaagatga tataagttat
      181 tgataaacaa ataggatagt ttaataaata tctgaaataa taaaaatatt tatgaaatta
      241 tttaatattt tataagataa taggtctacg aatagagttc taaactttca ataattgtat
      301 ttttagcgat ggctctcttc tatctaatat atggctctac ttatgctttc atcgttacaa
      361 attaaaaatt tccgttctct aaaaaacgta gagattccca aactcgggtt gatcaatctt
      421 attgttggta ataacaattc ggggaaaagt tccttaatcg aatcattgtt gattctcgcc
      481 aacaatgcag atgaagaaat tttaaatact ttagcttttt cacatggaga accaactctc
      541 tctgatcgtg aggaagaaga gtttactagt ccatttcaac ctttcgagag ctttttccca
      601 aataggcaat ttccaagaga agataatgta aaaattgttg ttggcgagct agactcctcc
      661 aattttctta ccattgaaca tgcctttgaa aaagagtttt tgatcaagtt tgaagatgaa
      721 gaagggaata ttttaagaag acttaagcgt gatgtgatct caaaattaga aattgatgag
      781 atgctagaag accctgaaca aaatttacaa attaatagta ttttaaaaat tgaaaataag
      841 ggatctgtta gccgtatttc attagagcca gatagacgta gagcaactac aattagtaga
      901 agcctagatt caaaattaaa tattccctat agctatatcc ctacatcatt tttagatcca
      961 gatgaactag ctctagattg ggataagtta gttttaactc catatcaaga ttatattatt
     1021 gaagccttac agattatcga gcctaattta gaaaatatta gtttcattaa atcagtaaat
     1081 taccgtagat ctagatatag aagctcacag agaacaccta ttgttaaatt aaaagatcat
     1141 agtcttcctt tcccactaag tagtatggga gatggtatct taagaattct tcaattagtt
     1201 cttaaattac atagtgctcg aggaggaatt ttattaattg atgaatttga taatggtttg
     1261 catcacagtg tccaagaaaa agtttggaaa cttgtttttt ctttagcaga aagtcttaaa
     1321 attcaagttt ttgcaacaac tcatagctct gactgtgtta aagcattttc aaaagtttca
     1381 aaagaacgaa cggatatcga aggtattcta attcaaattg ggaaaagtac acgtaaaagt
     1441 aactttgggg aagttattgc aaatattcta gatgaaaatc aattagccac atttattaag
     1501 tcagattttg aagtacgatg aaacatttaa ttgtagaagg acttggagac aaagtctttt
     1561 ttgaaaaata ttgtcaatat tactcttata atgttgatgt aaatatcctt acacctgaag
     1621 atattaatcc acaatactac acaactaaaa atggtgttat ttcatcatct ttggatgact
     1681 taattagtca aatcacatta ggtattatta aaaatctagg tatagtaatt gatgctgatt
     1741 atattaaaga caagtctggc attgaaaaaa ctaaagaaag tttaactcaa aaactagcac
     1801 ctcatgggta tatgctagta gaaactgatg aaggaatcat tgctaagcat aatgatggtt
     1861 taattgatat aggtatatgg ataatgccta ataatgatga ggatggaaac attgagaatt
     1921 ggatagctga actcatcagt gaatctgaaa cgcccttata tgaaaaagct aaactatctg
     1981 ttagtgaatt agctgaagag ggcttacaga aatttaaacc ttctagagta caaaaggcac
     2041 aaatagcaac ttggtttgct tggcaaaaaa gcccaggaca tggattagat ttctttttta
     2101 atgctcccct aattgatatg aatcatatta aatataccaa tttttgtacg tggtttagac
     2161 gaacttttga gattcaataa caatctttct tagtttaagc ataggtacct atctgggtgc
     2221 cttgtgactt aacataaaat ctcatctacg aaatccagat tcaagacata aaaaagcccg
     2281 acatcctgtc gggctttttt agattgggtt gctcgtcttg ctgatctcgt gctgcccatt
     2341 ccttgcgcta ctttcttatt cttgtttttc tggaaacatc ctgttcccta tatagccatc
     2401 ataggattaa tgccttgaat taccaatccg caaaatcctt aaatctatgt aagttttagc
     2461 ttacaaaatt ggcctatttt atcccaagag ttgtccgcag aaattgggga ttatttctaa
     2521 agtgtgcccc ttgagaaatc aggaaaaaac gccaaaacga atgttttttg atcaatttta
     2581 ttggctaaaa gttacagatt tgcttaataa aaagccgact tgtttccaag tcggcttttt
     2641 aagttttgcg tcccaatttt ttatagcata actttttgaa atataaaact atttataagt
     2701 gcgtttcgta taagctctat tatgttaaat aattaaggat aaaagcttta ttactaaata
     2761 gaacagttgc ttaaagcagt atagagttaa gtttgcattt taatatcaag ttgtctttca
     2821 gcaccgataa tcaccatacg aatcccattt tcaaaaatgt catccgcatt catattttgt
     2881 aaggtattca ctccttggag catgagtgga tatatagttg gatcggcttc gaatggtggg
     2941 tctgatctta tttcttcttg ctgctcctca aggacccacc caacaataaa ccttcctaat
     3001 gtcataagta atgcaactgc ttcaacagga gaaaacccag catgagataa cagcgcaact
     3061 tgggcctcaa tcgtgttgaa atgtccttga ctggggctag ttcttgcatg tagacgagca
     3121 ccatcacggt aagctagtag tgctcgacga aagcttacag aattagcgag caaccaatct
     3181 tgccatgtca tcccatcgat gggtaaagaa actaaatggt gttcatttat aatggtttcc
     3241 gccattgctt ccattaacaa ggatttattc ttgaaatgcc aatatagagt tggagattta
     3301 atacccaatc gttgagctag cttgcgtgtg gttattgaat ctatgccttc ttcattcaac
     3361 atatccaatg cggtacgcag gactatttca cgtgtcagac gttgaggtgc aatatctttt
     3421 ttactcataa atctatcact gatagggagt gtgtgagaga atattaattc tatcattgct
     3481 agagaaggca tttgtgaaga aatcattgag cgtgatttta atcactatat ttctggatgc
     3541 tgttgggatt ggtttaatta tgccgatctt gcctgaatta ttacggtcat tggctggagc
     3601 tgaagcaggc ggtgttcact atggtgcttt attagctgtg tatgctctga tgcagttcat
     3661 ttttgcacct atccttggag cgttgagtga ccgatttgga cgtcgacctg tattaattat
     3721 ttcaattgct ggtgcaacgg ctgattatct cctaatggct gctgctcctt ctctattgtg
     3781 gctatatatt ggtcgtattt ttgcgggaat tacaggtgcc aacatggctg ttgcaacagc
     3841 ttatgtttca gatattactc cagcccatga gcgtgcaaaa aggtttggtc tccttggagc
     3901 tgtctttggt attgggttta tagcgggtcc ggtaataggt ggagttttgg gtgaatggaa
     3961 cttacatgca ccgttctttg ctgctgcttt tatgaatggg attaatttaa taatgacagc
     4021 agtcttatta aaagaatcaa aacacagcaa taaaatgact gagaaggttc aggagcaatc
     4081 aatattaaag aaattatcct atttgatcac tcaacctaat atggctccat tgcttggtat
     4141 ctttttaatt atcacattgg tttcacaagt ccccgcaact ttatgggtta tctatgggca
     4201 ggatcgttat ggctggagta tatttattgc aggtgtttcc cttgctagtt atggaatatg
     4261 ccattctatt gcacaggctt ttgctatcgc ccctatggta aagaggtttg gagagaaaaa
     4321 tacgttgtta tgtggaatag cttgcgatgc aattggttta cttcttttat ctattgctgt
     4381 tgaagaatgg gtgccttttg cgttgttacc attgtttgcc cttggtggag tagccgttcc
     4441 tgctttgcaa gcaatgatgt ccagaggtat tagtgatgaa agacaaggtg aattacaagg
     4501 gctattaagc agttttaata gtctgggggc tataattggt cctgtattag ttactagcct
     4561 ctattttatg actcaggcat cagctcctgg aatggtatgg gcattagctg caatacttta
     4621 tgtaatcacc ctacccttat tgcttaagta tcgcctgaat aaatattctg gagttccata
     4681 aaatatttaa tttattggtt taaaaactaa tggattctat agtttatcta aaattaacat
     4741 aatacacctt atacgaaatg cgttataatt atctatagaa atcaatgctg tagtttctag
     4801 tttatagccc aaacgtccct gttcgggctt tttttatgat ttttcacgtt ccacgcttgt
     4861 tatttaatca aattttcacg actttgttca tatcagttaa acaatcgtgg ttaatcatag
     4921 ggtctaaaaa aacggctcca aaagcccctt ttctccggct cctgttcaac gtgctccggc
     4981 actggaatcc gcttattttc tgtttcgacc ggagtagtca atccgtcatc tttctggtct
     5041 ggttctagtt taggattctc tgtcatttca gaatgaacag gaggagcaac tttgtgctct
     5101 tctacctctg tttttggttg ttcaagctga cctttaaact caattctatg agtcaaatcc
     5161 tctatgtgtt gcatcaaagc cttttctctg tgattggcta agtccaattg atttttgagt
     5221 agagaaattt gatctttcaa tacattttca atctcagatt gaacacgtac ttgtacatcc
     5281 ttctgaacgt gttcagtacg tgtattggac tgaacaccga taggattacc atagaccctg
     5341 ataagttcgg ataaatctat aagcccatca tgatcccttg ataattcacc attttttata
     5401 gcttcatata ttgttgttcg tgatttttta aaagctttac ttgcgtccat gacgctgaat
     5461 tttgttgtgt tcaatgtaat ccctcagtgc atgtacaagg atatttagat gaacatccta
     5521 cctgaacatg aagtttattt tgtactaaat cctacttttt taagatgtgg cagaagctct
     5581 tcaaacttct ctttatcttt tagcatttca gcaattcgaa ctgcaaattg tgggtagctt
     5641 tctgtacctt gagagtattt attcatgtct ggaagttcag aaagtttatt agcaaaacgg
     5701 tgacgttgtg catcggtcat ttttgagaaa agttcaagtg aagttgaact tttagaggtg
     5761 atcactttct tttgcttaaa tgtgaaagag aatcctgaaa tgttacggcc ttttttatgt
     5821 tgctcacatt taacgttaat gtctgtgtgc tcattaactt gggcaatagc tagatttaac
     5881 actcgatcct tgaagtttcc cattctagtg tattcatcat cgagaacacc tatcttctgc
     5941 ctgaactctg ctagttcaat aacaggtgtt tggccagtac tacgccaagc aatcaataat
     6001 tcgtataaac gtacagcgta agcactacta agatcactaa tctgctgtag ctcatatttt
     6061 gtgaattgtt cttctaaacg tgtaataaga ggcacgattg caggtgcaaa tattaatttt
     6121 actgttgctt catcatcaat gtatctaatt tcactgaccc agcgacttag gacattctca
     6181 atatttcctc ttttatttac ttcttgatag ctaaattgac gtgcgaataa gtctttacac
     6241 gcatctttga gggcttgata agctgtttgt cgtgcaacat taaactggtt tacataactc
     6301 tctgcatgaa cctctaatgg gtcatttgca ttgatacctc tgccactacc tcttgcctcg
     6361 acaatggcca ataggattaa acgttgttct actaaatcca aattatagct agcatttatt
     6421 agagcattat ctttaacaac taaatcccgc atcttattta tctacattat tatttatgaa
     6481 tataagtcta tattaataat tatgtagatt tatgcaaggt aaacgtagtt aaatctacaa
     6541 aaaatattat gaagtcttat ggtaggtaaa cgtagtcata ttggtaggta aacgtagtca
     6601 tattggtagg taaacgtagt catattggta ggtaaacgta gtcatataag ctctacatgc
     6661 ctttaaaata aaggtgttca gctagtctaa aagcatttaa aaactttaaa attattaaaa
     6721 gccctggcaa agaaaaattg cccttggttt tcgctacgct caaactctat tgaatctcgc
     6781 tttcgctcga ttcgtggggc aattttttag atcatcctgt tgtgactttg agaaaaagca
     6841 aaggcaagag caaaagaaac tcggaattcg cttcgctcat aaagcttttt tactcgctac
     6901 gctcgatcta ggcacaattt tctgcatgtg atttacgctc atttagactt cacagcctaa
     6961 aaagctgttt ttcagagagt cgagatgcga attttgttgc ttcgctcgta gacactcact
     7021 ctattcatcc cgattagaat aaaaatagcc tctatttggc gctcatttgc gttttttgac
     7081 ctgattactc atttcaatag tggattctat gtcttaagta aaaatgtggc tgaaatcgca
     7141 tgtgagagcg ttttaacttg ggttttattt ttgacctgta ggctaaagtt tccgcttata
     7201 ggtttttctc ttaagaaaca caatagtcag gatctctttt ggccaagcac tatctttact
     7261 gtcagttaag tcaattaaat ggatggggag ccggtacata ccggcaattt ttggttcaca
     7321 caccacgcat ctaaagataa aggcttcaaa gccttatgct ataagggata cagcgatttt
     7381 tcatctgtct ggaaagcggt tacgctttta tgctgatttt tattattttt cataggttta
     7441 gctgcaaaca ctttttgttg cagataaagt gcaatattta cttacaaaac aataagatag
     7501 cttgtaatca cctttgttta cgcttttata taaatttctc tttttatatt agatatttaa
     7561 cctgtaatca ttaaaactta acaaagtgtt tacttatatt tgatagttac tttttagaaa
     7621 ttaggtgctt agattgaaaa caaattgtag gagtttttca tgaacagtac tcgaaccgtt
     7681 gccgtcagat tagacaatca ggttgcacaa gaatatgagc gtttggctca gttgaagggt
     7741 gataaattag ggacttatct gcgggatctt ctgaccagta atttacagat ggccagtatt
     7801 gaacggcagg ccagtcggat tgaaacattg gtgaatgatt tcgaaaagca gattaattat
     7861 tccctcgata agtttactga agccaattat ctgaacaaaa aattctttga agattttggt
     7921 ggggtataca tgatgctact cagtacgctt atgcatttaa aagtagaaag agatgatatt
     7981 cgcggtatgc aaagacaagg aaatgcgtat gcccaaagga actattttga ggggaaaaag
     8041 ccatgagcaa tattattcaa ctctctaaac catgtgcttt ctgtgattca agggataacg
     8101 tacagctttt tgcagggcta atgctttgcg gaaattgtca ggaaaacatc cggatcacta
     8161 atcctggcat gtttgaggcc aataatcaga ttgaacaaaa agcccaggat taacctggtg
     8221 ctattcacca attaaaatgt ctatgcgata aaccatttaa aatgtcctgt ttttaactgc
     8281 aagagtcaag aacaggattt aaatatcttt gttcaataac agctttctga gctttacgac
     8341 tcggcatact tttcttgaga cgggaacgtt tattctgttt ctccaactcc tcatgttgct
     8401 gctggatatg ggccagaacc gagcctaatc ttttattctc aaccactgca ttttgctgca
     8461 gtccagccag tttattgaag acgctatagt tgatcttccg tccttcatgc atgattgcca
     8521 cagtaccatc cgggtactcc agaaatgaaa tgtatagccc aacaagctta tgattcagct
     8581 ctgtcggttc aagcatataa atacacttgt cataggtcag ggtcagattc ttggtgacct
     8641 tacgcggttc ctgccaggta aaaatatcat ccagttcaag atcagattcg cttaatggcc
     8701 gatgcaggtt ctttgagttt cgcgcacatt tggcgaactt ctgattgaac tgctcaataa
     8761 agcagggtaa ccatatattg gcctctgcaa tcgaaccgat gccttccagg cgcatctcct
     8821 tgatcagacg atcctgaagt gtcctgttgg cacgttccac acgacctttg gcctgtggtg
     8881 agttggcaaa gataatatca atgtttaact cattcagaat ccggccaaat tgcgtgatct
     8941 ggctgtcctg actggatttc tggttcactc tgaataccga atgtttgtcg ctgtaaaagg
     9001 ccagcggctt accgtattgc tcaatgtagg ctcgggtgga aagcatatag tcaaaggtgg
     9061 tttcagcctc acaaaagcgc agatgcaaca gctttccagt ggcatcatcg atataaacca
     9121 acaagcagca tttggctgct cgtccttcaa accagtcgtg atatgagcca tcaatttgga
     9181 tcagttcacc gaagcaatcc cggttataac gtggctgata tggacgcttc aggcgcttgg
     9241 atcgcggaat ccacaggtca ttggccgtca tccagcgccg aagcgtttct accgggatat
     9301 tcaggtcaaa catgctgctg agcttctcat gcgccaaggt gggtccaaag cccagcaaat
     9361 gttcagcaat aatggaaagg cactttgatt ttaacaagtc atcatggcga cgatggccag
     9421 gttgaccacg actggcatgt gccatacctg agacaccatc ttgattgagc ttctgcaata
     9481 accgggtaat ttgacgggtg gaaagattta ggatttcagc agcacggacc tgggtaatac
     9541 gatgatctcg aacgtcttgc agaactgcaa gccgttgaat ttctttgtca gacatagtga
     9601 tcagcatcta tatttatatc cagtccatgg tgataaaaac catcataaac tagacatttt
     9661 tattggtgag aatatagaca ctttaaatgg gttctaaca
//
