LOCUS       NZ_CP035938            12247 bp    DNA     circular CON 22-APR-2024
DEFINITION  Acinetobacter cumulans strain WCHAc060092 plasmid p3_060092,
            complete sequence.
ACCESSION   NZ_CP035938
VERSION     NZ_CP035938.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN08772519
            Assembly: GCF_003024525.3
KEYWORDS    RefSeq.
SOURCE      Acinetobacter cumulans
  ORGANISM  Acinetobacter cumulans
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter.
REFERENCE   1  (bases 1 to 12247)
  AUTHORS   Qin,J., Maixnerova,M., Nemec,M., Feng,Y., Zhang,X., Nemec,A. and
            Zong,Z.
  TITLE     Acinetobacter cumulans sp. nov., isolated from hospital sewage and
            capable of acquisition of multiple antibiotic resistance genes
  JOURNAL   Syst Appl Microbiol 42 (3), 319-325 (2019)
   PUBMED   30808586
REFERENCE   2  (bases 1 to 12247)
  AUTHORS   Qin,J., Long,H., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2019) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
  REMARK    Sequence update by submitter
REFERENCE   3  (bases 1 to 12247)
  AUTHORS   Qin,J., Long,H., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-FEB-2019) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
REFERENCE   4  (bases 1 to 12247)
  AUTHORS   Qin,J., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAR-2018) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003024525.3-RS_2024_04_22
            Annotation Date                   :: 04/22/2024 04:03:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,515
            CDSs (total)                      :: 3,393
            Genes (coding)                    :: 3,310
            CDSs (with protein)               :: 3,310
            Genes (RNA)                       :: 122
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 96
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 26 of 83
            Pseudo Genes (incomplete)         :: 44 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 11 of 83
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP035938.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12247
                     /organism="Acinetobacter cumulans"
                     /mol_type="genomic DNA"
                     /strain="WCHAc060092"
                     /isolation_source="Sewage"
                     /type_material="type strain of Acinetobacter cumulans"
                     /db_xref="taxon:2136182"
                     /plasmid="p3_060092"
                     /geo_loc_name="China: Chengdu, Sichuan"
                     /collection_date="2018"
     gene            join(10876..12247,1..3608)
                     /locus_tag="C9E88_RS17340"
                     /old_locus_tag="C9E88_016600"
     CDS             join(10876..12247,1..3608)
                     /locus_tag="C9E88_RS17340"
                     /old_locus_tag="C9E88_016600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005204747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type ISP restriction/modification enzyme"
                     /protein_id="WP_106986721.1"
                     /translation="MTALTQLLEKIQDVSVTQRDKGTSFENLMVQYFLNEPKYAELYTE
                     VLSYSDWVEKYGEKLNITDKRDYGIDLVAVTIEGDFHPIQCKNYNTTKIQKKDIDSFLG
                     GSGKSYFAYRYIVASTDDWSDNAKSSLVDAQPPVATISLLDLEGSLIDWSQFDFDPNVK
                     PVFRDKKQLRQHQRPALNAVKHALTTADRGKLIMACGTGKTFTSLRIAEELAGRGKNVL
                     FLVPSLALLSQTLDEWTQDTMIGIRSFAVCSDSDVGKKKDDDNIVVGISDLKFPATTNP
                     QSLVRAFKARDLFKPNEANEDMNVVFSTYHSIDVIAEAQKAGFPEFDLIICDEAHRTTG
                     ATFEGDDESKFVRIHNNDYVQGKKRLYMTATPRIYGDTAKETEGVTLCSMDDKSLYGED
                     LYVITFSQAVKMGILCDYKVIVLAVEESHINRQLQSLLKDENNSLKVDDAAKIVGCWKA
                     LAKQGLYNPDGTVAKPMERAVAFCQVIEKEYKGKKHKVSSKQITEMFSAVVEAYQQAEM
                     DHLYRTEEDPYIDLSLKLNCQVKHVDGGMNATEKKEKITWLKEQAPENTCRILSNVRCL
                     SEGVDVPSLDAVLFLTPRNSQVDVVQSVGRVMRKAPNKELGYVILPVVIPAGVEPEQAL
                     NNNEAYRVVWQVLNALRAHDDRFDAMINKLEFNGKDVSRMEVVAIADKVVKKAKKVKAQ
                     ATDGNETSVQSGLSEGKKRKAKAQARGGSSIGQAASPTPQQTEMEFEVGEIERALYAKI
                     VKKCGNRHHWEDWASDVAKIAKTHIDRIQAILENPTNTNEIAAFNEFAHELRDDLNNSV
                     SDAEIIEMLAQHLITKPVFDALFKDHSFAEHNPMSLAMQKVLNALNEHHLEKEADTLNS
                     FYESVRMRAEGINTAEGKQSIILTLYDSFFKNAFPRMTEKLGIVYTPVEVVDFIIHSVN
                     DVLKSEFGKTFADDNVHVLDPFTGTGTFINRLLQSGLIPADRLTYKYKNEIHANEIVLL
                     AYYIASINIETVYHDQMKTVNPDLEYAPFEGICLTDTFEMYEKDDLVSKVLVDNSARRK
                     RQKNLDITVIVGNPPYSAGQESANDNNANVAYPHLDERIRTTYAEHTTSTNKNSLYDSY
                     IRAIRWASDRIQDCGVIGFVTNGGYIDSNSADGLRKCLADEFSSLYFFHLRGNARTSGE
                     QRRKEKDNVFGQGTRTPILIAVLVKNPNAKQYGQIYFHDIGDYLSREEKLEKIADLVSI
                     NGITEVDGWTKITPDTHNDWLNQRDDSFNEFIEIGNKKDKNSISLFDSYSKGLMTGRDA
                     WCVNSSSLKIISNLNKMITVFNNHVSENKNSNDNLTNDPSKISWNRSLLQTLDKGLSLN
                     IDKINLRKYLYRPFTKTTVYFEKNLNEMTYRIPSMYPLNHNNLSIIINGIGVNKAFSAL
                     LTNAIPDVQNHANCQCFPLYLYEETSTENKADDLFSHAETNTADGQYTRKDAISDEGLK
                     HFQDAYPTETISKEDIFYYVYGLLHSEDYRTRYADNLTKELPRIPCVKKSDDFWAFSKA
                     GRDLAHWHLNYETVEPYKAKLDLGGKSLKQLEDKDFYVTKMKFPKKDQKDTVVYNNSIT
                     IREIPLEAYDYVVNGKSALEWVMERQGVSTHKDSGIVNDANDWAIETMGDAKYPLNLFL
                     RVITVSLETMKIVKTLPKLDI"
                     /name="type ISP restriction/modification enzyme"
     gene            3780..4172
                     /locus_tag="C9E88_RS17345"
                     /old_locus_tag="C9E88_016605"
     CDS             3780..4172
                     /locus_tag="C9E88_RS17345"
                     /old_locus_tag="C9E88_016605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_171501797.1"
                     /translation="MTNRFKAARQNTEFTHNDVNSDVKENIEQSSKINIVTRNQSVITI
                     EKNTLPVAKRVRTKHGRNLTTPLFVEELTAIEEAVKKLGQEQDISMATFIRQTILDQCK
                     KVLGKEGFNEIMANQLNSVKPKKEKE"
                     /name="hypothetical protein"
     gene            4240..4848
                     /locus_tag="C9E88_RS17350"
                     /old_locus_tag="C9E88_016610"
     CDS             4240..4848
                     /locus_tag="C9E88_RS17350"
                     /old_locus_tag="C9E88_016610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807825.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="WP_106986724.1"
                     /translation="MAKIICVANRKGGVGKTTLATNLAVALNNKGKSILIDADEQQSAS
                     KWAKQRNDIECISVHSELLTKLEEIDSKYDYILIDVAGRDSTIFREALLVANTLIVPTQ
                     PSLLDLDVLEYMQDKIATARKMNSDLDAYVLINRASPRSNELPDAIDFIKDFDQFKLLK
                     TVLFERKQFRDAIIESKSVTEMNSSKAKDELNKLIIEIL"
                     /name="ParA family protein"
     gene            5645..5917
                     /locus_tag="C9E88_RS17355"
                     /old_locus_tag="C9E88_016615"
     CDS             5645..5917
                     /locus_tag="C9E88_RS17355"
                     /old_locus_tag="C9E88_016615"
                     /inference="COORDINATES: protein motif:HMM:NF013855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_162368856.1"
                     /translation="MIEKVQQLPFLTERQTSVLRFLYDYMSQHRYMPTRREVAQFLKLK
                     SDNASAYLSPLEKKGYLVRSEHTRRNLELTDLAYEKLNLTQNETL"
                     /name="hypothetical protein"
     gene            6049..8076
                     /locus_tag="C9E88_RS17360"
                     /old_locus_tag="C9E88_016620"
     CDS             6049..8076
                     /locus_tag="C9E88_RS17360"
                     /old_locus_tag="C9E88_016620"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_137287952.1"
                     /translation="MRQLTEKQIINQNSLFELHQNVCFHADRVLSNQSEKSILHLKDFS
                     QRTCRLLCQFISDDANIFDILNDPEIITILDSFYKELANNKSITTVNSSRRAMNFILYE
                     VWGYYKTKHWLPLHSKKFDEKLNVLDRFLLCIEAAKEIISLSYPNISTDRAYKYFMAQI
                     NDICGGNYVTSYSLRMNFYRKKMPIWVNDNIDIKPDFYLGKEHKPICIAKAIEKLSGAP
                     KGYLIDLINISEIKKEKLSRIGVRADSFDILLINSTFRKELKLYAEYKTSAIINKKRNE
                     KWTVRLQNNKTKQKYKTIMNATLIKELCVLNPEQSIPSFEVLIGYIIRISRYALDLKLL
                     KADELSLYTVTQAEISEKILNHLINKDQFIPSHMYNYLSSFAALWFSNSPFFVEFADEV
                     FSTRYNLSKDQLLQVSKDNQDRFKKLQSQVKSYVKQSKNSHKLLNRNVLELENPSMYIL
                     DILKNAKLDLNSVLDSKELSSNSATLIRNICIVSCLLCCPLRVRNWVNMKIGYHQDDEC
                     IYFDDNKKSYVLSIPKSQFKNFKQKNIPDVFSLVILPQFTQAITDYINEARPLLLKGEQ
                     SDYFLVSQHSSKIDTGSLNKMIKQFTYQYDDKNLRIGGINIHAFRAIVATTFLKKFKGS
                     FAYAAFLLLDSEETIRESYGHLSPDDAFAEWGKIISVEAA"
                     /name="hypothetical protein"
     gene            8076..10766
                     /locus_tag="C9E88_RS17365"
                     /old_locus_tag="C9E88_016625"
     CDS             8076..10766
                     /locus_tag="C9E88_RS17365"
                     /old_locus_tag="C9E88_016625"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_106986720.1"
                     /translation="MNSYKYDFEDINNRLIQSVDTICSHFWSNGKKQGIYWVVGDVYNT
                     KSSKDGSFKITLSGAKKGLFHDFNGDKHYSPLDALAALYSVDVLEAAKIASSFLGLSVE
                     ERKNLKFVKPQLKVKEPDSKNTILPKKLNVSWSKKLEQRLAKNEVALAYLYGRSLTAET
                     IKEFRLGLSTPYKNKDGVVSADALVYPIRCCNGDFLSSNGYYNISDLTQNPIDKNGWMK
                     GSAQPYFTHAYTSQDAIFICEGVKDAWVIWQNLKDFNLLDKILVCTSTHGSAIPDAMKN
                     ESFWTRFETIYLAHDHDSAGDVMAEKLMPFIGRDAFRLKVPPHFAKPKYSVDGVLKDES
                     EWGADWTDFFKANPGKTEFINHIQNAPLFSIRLEKASSNEELSVGRFNVAPVDINGAYL
                     NGFMYYPIQTLVRKQEEDEYGSSFVSERYETVVVRSDKTVHKAIKSPAPKGTPDSERVI
                     RLTDGTLIRSEPKANDYGTWKWNHIDAYINGKLKIRDIKSIFDDIYNFLKKSVWLPVKE
                     DYTILSLTVLATYVQNIFESVPLIFLNGQAGTGKSQTGIIMSKLSCNGSVIGQVSAATA
                     ARHIDAARGFVVFDDLEGVASKGGKESQFSDLVQALKVSYNKHTAEKVWTDVKTMKTER
                     LNFFGIKLLSNTSGADDILSTRMLRIQTLYMPDSEKGTIKKMNASDFTRIENLRQELHA
                     WAFENCQKIEKIAQTHDEKTGRFDEIALPLRVIGSMISEECCKELEIALSKQRTVVFES
                     NEPVEVLKEAVKNIIKDGFHRFTVTQLIMELRTLLDDNFGANMTNQIPEWQRPEWIGRQ
                     LRTLGLLDNVDLGRTTFYGKRLKVMQFNDYAINQVIEELGATIDHIKEPFDFCKGCSSC
                     PYANSHCDFRSMRLQEEQKSKGKYS"
                     /name="hypothetical protein"
ORIGIN
        1 cactagctaa acaaggttta tataatccag atggtacagt tgcaaagcca atggaacgtg
       61 cagttgcttt ctgtcaggtc attgaaaaag aatacaaagg taaaaaacat aaggtaagtt
      121 caaaacaaat tactgaaatg tttagtgctg ttgttgaagc ctaccaacaa gcagaaatgg
      181 atcatttgta cagaacagaa gaagacccat acattgacct atcattgaaa ttgaactgcc
      241 aagtaaaaca tgttgatggt ggaatgaatg caacagaaaa gaaagaaaaa atcacttggt
      301 taaaagaaca agctcctgaa aacacatgcc gtattttatc gaatgtacgc tgtttatcag
      361 aaggtgttga tgttccatca cttgatgcag tgcttttcct tacacctcgt aactcacaag
      421 tagatgtcgt tcagtctgtg ggtcgtgtca tgcgtaaagc tccaaataaa gaattgggct
      481 acgtaatttt acctgtcgtt atccctgcgg gtgtagagcc tgaacaagca ttaaataata
      541 acgaagccta tcgtgtagtg tggcaagtat taaatgcctt acgagcacat gatgaccgct
      601 ttgatgccat gattaataaa cttgagttta atggcaaaga tgtatctcgt atggaagtcg
      661 ttgccattgc agataaagtt gtaaaaaagg cgaaaaaagt aaaagctcaa gcgactgatg
      721 gaaatgaaac atctgttcaa tcgggtttaa gtgaagggaa aaagcgaaag gcaaaagccc
      781 aagctagagg tggatcatct attgggcaag ctgcctcacc aacgccacag caaaccgaaa
      841 tggaatttga agttggtgaa attgaacgtg ctttatatgc caaaatcgta aaaaaatgcg
      901 gtaatcgcca tcattgggaa gattgggcta gtgatgttgc taaaattgct aaaactcata
      961 ttgaccgtat tcaagccatc cttgaaaatc ctactaatac aaatgaaatt gcagcattta
     1021 atgaatttgc acatgaacta cgtgatgact taaacaatag cgtatctgat gctgaaatca
     1081 ttgaaatgtt ggcacagcat ttaattacta aacctgtatt tgatgcactc tttaaagatc
     1141 atagctttgc tgaacacaac ccaatgagct tggcaatgca aaaggtactt aatgccctta
     1201 atgagcatca cttagagaaa gaagcggata cgcttaattc cttctatgaa tcagtacgta
     1261 tgagagcaga aggcattaat actgccgaag gcaaacaaag tattatctta acgctatacg
     1321 attcattctt taaaaacgcc ttcccacgca tgacggaaaa actggggatt gtttataccc
     1381 ctgttgaagt cgtagatttt attattcata gtgtgaatga cgtactcaaa agtgagtttg
     1441 gtaaaacctt tgctgatgac aatgtgcatg tacttgatcc atttacgggt acaggtacgt
     1501 ttatcaaccg tttgcttcaa tcaggtttaa tccctgctga tcgtttgacc tataaataca
     1561 aaaatgaaat ccacgccaat gagatcgttt tattggcgta ttacattgcc tcaatcaaca
     1621 ttgaaaccgt atatcacgat cagatgaaaa cagttaatcc cgatcttgaa tatgcaccat
     1681 ttgaagggat ttgtttaaca gatacctttg aaatgtacga aaaagatgac ctcgtaagta
     1741 aagttcttgt tgataacagt gcaagacgta aacgccagaa gaacctcgat ataacggtta
     1801 ttgttggtaa tcctccttat tctgcaggtc aagaatcggc aaatgataat aatgcgaatg
     1861 ttgcatatcc tcatttggat gaacgcatta gaacaaccta tgctgaacat acgacttcaa
     1921 ccaataaaaa ctcactttac gattcctata tccgtgcaat acgttgggcg agtgatcgta
     1981 ttcaagattg tggagttatt ggctttgtga ccaatggtgg ttatatcgat tcaaattcag
     2041 ccgatggttt acgcaagtgt ttagctgatg aatttagttc tttatatttc ttccacttac
     2101 gagggaatgc tcgaacatca ggcgaacaaa gacgtaaaga aaaggataat gtttttggtc
     2161 aaggcactcg aaccccaatt ttaattgcag ttttggtgaa aaatccaaat gcaaaacaat
     2221 atggtcaaat ctattttcat gacatcggtg attatctaag tcgtgaggaa aaattagaga
     2281 agattgctga cttagtaagc atcaatggca ttacagaagt tgatggttgg acgaagatta
     2341 cacctgatac acacaatgat tggttaaatc agcgtgatga cagttttaat gagtttattg
     2401 aaattgggaa taagaaagat aaaaattcta tttccctatt tgattcatat tcaaaaggat
     2461 taatgacagg tcgagatgct tggtgtgtta attcatcatc actaaaaatc atatcaaatc
     2521 tcaataaaat gattaccgtt tttaataacc atgtatctga aaataaaaac agtaatgata
     2581 atttaactaa tgatcctagt aaaataagtt ggaatcgtag tctacttcaa acattagata
     2641 aaggactaag tcttaatatt gataaaatta atttaagaaa atatctatat cgaccattta
     2701 caaaaacaac cgtatatttt gagaaaaatt taaatgaaat gacatataga ataccatcta
     2761 tgtatccttt aaatcataat aatttatcaa tcattataaa tggaattgga gttaataagg
     2821 cattttctgc tttattaaca aatgctatac ctgacgtaca gaaccatgca aattgtcaat
     2881 gctttccact ttacctttat gaagaaacat caacagaaaa taaagctgat gacctattta
     2941 gccatgctga aactaacacg gcagatggtc aatatactcg taaagatgca attagtgatg
     3001 aaggtttgaa gcatttccaa gatgcttacc cgactgaaac gattagcaag gaagatattt
     3061 tctactatgt gtatggttta ttgcacagtg aagactatcg tactcgttat gcagacaact
     3121 tgactaaaga gttaccacgt atcccatgtg tgaaaaaatc tgacgatttc tgggcgtttt
     3181 ctaaagcagg gcgtgatttg gcacattggc atttaaacta tgaaacggta gagccataca
     3241 aagccaaatt ggatttaggc ggtaaatcat taaagcaatt agaagataaa gacttctatg
     3301 tgaccaaaat gaaattccct aaaaaagacc aaaaggatac ggtggtctat aacaattcta
     3361 ttaccatccg tgaaataccg ttagaggctt atgactacgt ggtgaatggt aagtctgcac
     3421 ttgaatgggt tatggagcgt caaggcgttt ctacgcacaa agacagtggc attgtgaatg
     3481 atgcgaatga ttgggcaatt gaaactatgg gtgatgctaa atatccattg aatttgtttt
     3541 tacgggtcat cacagtaagt ttggaaacaa tgaaaattgt gaagacttta cctaaattag
     3601 atatttaaag taaaaagccc tttcaggcag aaagggcttt tttcaaaaaa ggccaaaaaa
     3661 atacaatcat aaattgttgt acttttagtt ttagttttaa aactattttt agttgtattg
     3721 ttagttgtga aaataaagct ataattagta acaatgttaa acatgaatcg aggattaaaa
     3781 tgactaatcg ttttaaagca gccagacaaa atacagaatt cactcataat gatgtgaact
     3841 ctgatgttaa agaaaatatt gaacagtctt ctaaaatcaa tattgttact cgtaatcaaa
     3901 gtgtaattac tatcgagaaa aatacgctcc ctgttgctaa acgagttcga acaaaacatg
     3961 gcagaaatct tacaacccct ctttttgtcg aagaacttac cgcaatcgaa gaagcagtaa
     4021 aaaagttagg gcaagaacaa gatatttcaa tggctacttt tattagacaa acaattttag
     4081 atcaatgtaa aaaagttcta ggtaaagaag gttttaatga aattatggca aatcaactta
     4141 attcagttaa gcctaaaaaa gaaaaagaat aagtctatat ttaggtctaa aaatacaact
     4201 aatattaagg ctaattataa atgtatctgg agtaatataa tggcgaaaat tatatgtgta
     4261 gcaaatagaa aaggtggagt tggaaaaact acacttgcga caaatttagc agttgcatta
     4321 aataataaag gtaaaagcat tctgattgat gctgatgagc aacaatcagc atctaaatgg
     4381 gctaaacagc gcaatgatat agaatgcatt agtgttcatt cagaactact caccaaacta
     4441 gaagaaatcg atagtaaata tgattacatt cttattgatg tagcaggtcg agactctaca
     4501 atttttcgtg aagcactctt agttgcaaat acactaatag ttccaactca accatcatta
     4561 ttagatttag atgttcttga atacatgcaa gataagattg caacagcaag gaaaatgaat
     4621 agtgatttag atgcttatgt tcttattaat cgtgcatcac cacgatcgaa tgaactacca
     4681 gatgcaattg atttcatcaa agactttgat caatttaaac tgttaaaaac agttctattt
     4741 gaaagaaagc aattcagaga tgcaattatt gaaagtaaat cagttactga gatgaatagc
     4801 tcaaaagcta aagatgaatt aaataaacta attatagaaa ttctataaag taaaactaga
     4861 aaataagaaa gagtgcacat gcactctttc ttattgtgta gaaaaaaata aaaagcaaat
     4921 aattcataga gttttctact agaaattaaa aaatttttct actcagtata taaaaactat
     4981 ttattcataa taaataattg atttttttaa aaaaaaccat tcaaaaaatg aagcataacc
     5041 gcatccctta catgaaaata taaatacata caaaaccata taaaacaaca tgttatgttt
     5101 aaaaaagcat attggtgtag aaaggaataa aaaatacaaa gaaaaatgta gaaaaatgat
     5161 gcgtagaaaa agtatgtaga taattaaatt aaattaaaaa agcgaatcca attacaaata
     5221 aaactattta aaatcaaaaa gttagtgtat ggcatgctct cataactgca tgtcacaaaa
     5281 atataaaaat ataaaaatat aaaaatataa atcatatact tatgtatatt tattttttga
     5341 catactattc tgacacgcaa tttaattaat ggattcgctt ttcctgacat gcaattttgc
     5401 gtgtcaatag catgtcaata tagcatgtca tataattttt ttttcatctt attgttttac
     5461 atagttttat ttttcatttt gacatgcatt tactatgtga cacgcatatt tccaatattt
     5521 aaattatcct tttctacatt ttttttaata tttaatatgt atgatttttt agtaccgtaa
     5581 tattgtccaa aaaggtttta aaatgtatac ttttctatgt tatctaagta tcgtaggctt
     5641 tcacatgatt gaaaaagtgc agcaactacc gtttttaaca gagaggcaaa cctctgtttt
     5701 acggtttctt tatgactata tgagtcagca ccgctacatg ccaactcgta gagaagttgc
     5761 acagttttta aaattgaagt cggataatgc ttctgcttat ttgtccccct tagaaaaaaa
     5821 gggctacttg gttcgatctg aacacactcg cagaaatttg gaattaacgg atttagctta
     5881 tgaaaagtta aatctaactc aaaatgaaac gctataaata gaaaaacggc actgctgcta
     5941 acagtaccgt ttttgaatat ctcatctaac aatgcagtcg ctagataaga ttattctctc
     6001 atatttatag actcgtttca aattacctaa ctaacatagg ggtttgttat gcgtcaatta
     6061 actgaaaaac agataattaa tcaaaatagt ctttttgaat tacatcagaa tgtttgcttt
     6121 catgctgatc gtgttctctc aaatcaatca gaaaagtcga tcctgcatct taaagacttt
     6181 tcccaacgaa cttgtagact gctatgtcaa tttatttcag atgatgccaa tattttcgac
     6241 attcttaatg atccagaaat aattacaata cttgattctt tttataaaga gttagctaat
     6301 aataaatcta ttacaactgt taattcatca agacgagcaa tgaatttcat actttatgaa
     6361 gtatggggat attataaaac aaagcattgg ttgcctttac atagcaagaa gtttgatgaa
     6421 aaattaaacg ttttagatcg ttttctcctg tgtattgaag ctgcgaaaga gattatttct
     6481 cttagttatc ccaatattag tactgatcgt gcttataaat attttatggc gcaaattaat
     6541 gatatatgtg gaggtaatta tgtgacttct tatagcctta gaatgaattt ttatcgtaaa
     6601 aagatgccta tttgggttaa tgataatata gatataaaac ctgattttta tttaggaaaa
     6661 gaacataaac ctatttgtat agcaaaagcg attgaaaaac tttcaggcgc acccaaaggt
     6721 tacttaattg atctaattaa tattagtgag attaaaaaag aaaaacttag cagaattgga
     6781 gttcgagcag attcttttga tattttatta attaactcca cttttagaaa agagttgaaa
     6841 ctttatgcag agtataaaac atctgcaatt attaataaaa aacgcaatga aaaatggact
     6901 gtcagattac agaataataa aacgaaacag aaatataaaa caattatgaa tgctacatta
     6961 ataaaggagc tttgtgtttt aaatccagaa caaagtatcc catcctttga ggttctcatt
     7021 ggctatatta ttcgtattag taggtatgct ttagacttaa aactccttaa agctgacgag
     7081 ttatctcttt atacagtcac acaagctgaa attagtgaga agattcttaa tcatttaatc
     7141 aataaggatc aatttattcc tagtcacatg tataactatc tgtcttcttt tgcagcttta
     7201 tggttttcta attccccttt ttttgttgaa tttgctgatg aggttttttc tactcgttat
     7261 aacttatcta aggatcagct tttacaagta agtaaggata atcaagatag gtttaaaaaa
     7321 ttacagtcgc aagtcaaatc gtatgttaag caaagcaaaa attctcataa gttgctaaat
     7381 cggaacgttt tagagttaga aaatccatct atgtatatct tggatatttt aaaaaatgcc
     7441 aaattggact tgaattcagt attagacagt aaagaattat ctagtaattc tgcaacactc
     7501 atccgaaata tatgcattgt ttcatgtctt ctctgttgtc ctttacgagt aagaaattgg
     7561 gttaatatga agataggtta tcatcaggat gatgagtgta tatattttga cgacaataaa
     7621 aaaagttatg tcttatctat tccaaaatca caatttaaga atttcaagca gaaaaatatt
     7681 cctgatgtat ttagccttgt aattttgcct caatttacac aagcgattac agattatatt
     7741 aatgaagcta gacctttatt attaaaagga gaacaaagtg attatttttt agtgagtcag
     7801 cattcttcta aaattgatac tggatcactt aacaaaatga ttaaacaatt cacttatcaa
     7861 tatgatgata aaaacttacg tataggtggc attaatattc atgcttttag agcaattgtt
     7921 gccacaacct ttttgaaaaa atttaaaggt tctttcgctt acgctgcttt tttgttacta
     7981 gattccgaag aaactataag ggaaagttac ggtcatttgt caccagatga tgcctttgct
     8041 gaatggggca aaattattag cgtggaggca gcgtaatgaa ttcttataaa tatgattttg
     8101 aggatattaa taatcgtctt attcaaagtg ttgacactat ttgtagccac ttttggtcga
     8161 atgggaaaaa gcaagggatt tattgggttg ttggtgatgt ttataacact aaatcaagta
     8221 aagatgggtc ttttaaaatt accttaagtg gtgcaaaaaa aggtttattt cacgatttta
     8281 atggtgataa gcattacagc cctttagatg cacttgctgc actttattca gtagacgttc
     8341 ttgaagcagc aaaaatagct tcttcttttc ttgggttatc tgtagaagaa agaaaaaact
     8401 taaaatttgt aaaaccacaa ttaaaagtaa aagaacctga tagtaaaaat actattcttc
     8461 caaagaaatt gaatgtttca tggtctaaaa agttggaaca acgccttgca aaaaatgagg
     8521 ttgctttagc atatctttat ggtcgatctt taacagcaga aacgattaaa gagttcagac
     8581 ttggattaag tacaccatac aaaaataagg atggagtagt tagtgcagat gctcttgtct
     8641 atcctattcg ttgctgtaat ggtgacttcc ttagctctaa tggttattac aatatttctg
     8701 atctaaccca aaatccaata gataaaaacg gttggatgaa aggatcagca caaccatatt
     8761 ttacccatgc ttatacttcg caagatgcaa tttttatttg tgagggtgta aaagatgcat
     8821 gggtaatttg gcaaaatctc aaagatttta atttattaga caaaatatta gtttgtacta
     8881 gcacacatgg ttctgccatt ccagatgcca tgaaaaatga aagtttttgg actagatttg
     8941 aaactatata tcttgcacat gatcacgata gtgctggtga tgttatggct gaaaaactta
     9001 tgccatttat tggtcgtgat gcattccgct tgaaagtacc tccacatttt gcaaaaccta
     9061 aatatagtgt agatggagtt ttaaaagatg aatctgaatg gggagctgat tggactgatt
     9121 ttttcaaagc taaccccgga aaaactgaat ttattaatca cattcagaat gctccactat
     9181 tttctattag gttggaaaaa gctagttcta atgaagaact tagtgtaggt agatttaatg
     9241 ttgctcctgt tgatataaat ggtgcatacc taaatggatt catgtattac cctattcaga
     9301 cgttagtacg aaaacaggaa gaagatgaat acggtagtag ctttgtttct gagcgttatg
     9361 aaactgtcgt tgttcgatct gataaaaccg ttcataaggc aattaaatcc cctgcaccga
     9421 aaggaacacc tgattcagag cgtgtcattc gattgactga tggaactctt atacgtagtg
     9481 agcctaaagc caatgactat ggtacttgga aatggaatca cattgatgca tacataaatg
     9541 gaaaattgaa aataagagat attaaatcca tttttgatga tatttataat tttctaaaaa
     9601 agtcagtttg gcttcctgtc aaagaagatt acaccatctt atctttaact gttcttgcga
     9661 cttacgttca aaatattttt gaatctgtac cgttgatttt cctaaatggt caagcaggta
     9721 caggtaaatc ccaaacaggt attatcatgt cgaaactctc ttgcaatggt tcggtaattg
     9781 ggcaagtttc agcagctaca gcagcacgac acattgatgc tgctagaggt tttgttgtat
     9841 ttgatgatct tgagggggtg gcaagtaaag gcggtaaaga aagccaattt agtgacttag
     9901 ttcaagccct aaaagtttca tataacaagc atacagcaga gaaagtttgg actgatgtaa
     9961 aaacgatgaa aaccgaaagg ctgaactttt ttggcattaa gttacttagc aatacgtcag
    10021 gtgcagatga catcttaagc acacgtatgt tgcgaatcca aacactttac atgcctgata
    10081 gcgagaaagg aacaatcaag aaaatgaatg cttccgattt cactagaatt gaaaatttac
    10141 gtcaagaact acatgcttgg gcttttgaaa attgtcaaaa gattgaaaaa attgctcaaa
    10201 ctcacgatga aaaaacaggt cgatttgatg agattgcact tcctttgcgt gtgatcggtt
    10261 caatgatttc agaagaatgt tgcaaagagt tggaaatagc tttatctaaa caacgaactg
    10321 ttgtttttga aagtaatgaa cctgttgagg tgctaaaaga ggctgttaaa aacattatta
    10381 aagatggttt tcaccgcttt acagttactc aactaataat ggagttacgt actcttttag
    10441 atgataactt tggtgcaaac atgactaatc aaataccaga gtggcaacgt cccgaatgga
    10501 ttggtcgtca attgagaact ttaggtttac tagataatgt tgatttaggt cgaactactt
    10561 tttacggaaa aagattgaag gtaatgcaat ttaatgatta cgcaatcaac caagtaatag
    10621 aagaacttgg agcaactatt gatcatatca aagagccttt tgatttctgt aaaggatgtt
    10681 cttcttgtcc ttacgctaat tctcactgcg atttcaggtc tatgcggttg caagaagaac
    10741 agaagtcaaa aggaaaatac agctaattaa aagtttaaat ttaaaatcat ttttagttgt
    10801 atttttacaa ctaaaaatga tttatttttt taattatttc cattgaattg tcaattatat
    10861 tttagggtta ttaaagtgac agcgttaaca cagcttttag aaaaaattca ggatgtatca
    10921 gtaacacaac gagataaagg aacttcattt gaaaacttga tggttcaata tttcttaaat
    10981 gaaccaaagt atgctgaact ttatacagaa gttttatcgt attctgattg ggtcgaaaag
    11041 tatggtgaaa aattaaatat tactgataaa cgagattatg gtattgacct cgtagcggta
    11101 actattgaag gtgattttca tccaattcaa tgtaaaaact acaacactac aaaaattcaa
    11161 aaaaaagaca ttgatagctt tttgggtggg tcgggtaaat catattttgc ctatcgttat
    11221 attgtggcat caacggatga ttggtcagac aatgccaaat catctttagt ggatgcacag
    11281 ccgcctgttg caactatctc acttcttgat ttagagggaa gtttgattga ttggtctcag
    11341 tttgatttcg atccaaatgt gaaaccagtt tttcgtgata aaaaacaact acgccaacac
    11401 caacgtcctg cattaaatgc agtaaaacat gctttaacaa ctgctgatcg tggcaagctg
    11461 attatggcat gtggtactgg taaaactttt acttcgttgc gtatcgcaga agaattagca
    11521 ggtcgtggca aaaatgtctt attccttgtg ccaagccttg cattattaag tcaaacactg
    11581 gatgagtgga cgcaagatac catgattggt attcgtagct ttgctgtgtg ttctgatagt
    11641 gatgttggta agaaaaaaga tgatgacaat attgtagttg gtattagcga tttaaaattt
    11701 cctgctacta ctaatcctca aagtttagtt cgagcattca aagctagaga tttattcaag
    11761 cccaatgaag ccaatgagga catgaatgta gtgttctcta cctatcactc cattgatgtt
    11821 attgcagaag cacaaaaagc aggatttcct gaatttgatt taatcatttg tgatgaggct
    11881 catcggacca caggtgcgac atttgagggt gacgatgagt ctaaatttgt gcgtattcat
    11941 aacaatgatt atgtacaagg aaaaaaacgt ctgtatatga ctgctacccc tcgtatttat
    12001 ggtgatacag ccaaagaaac tgagggtgtt acactgtgtt caatggatga taaatcactt
    12061 tacggtgaag atttatatgt catcacattc tcacaagcgg taaaaatggg gattctatgc
    12121 gactacaaag taattgtact tgctgttgaa gaaagccata ttaaccgcca attacagagc
    12181 ttattgaaag atgaaaataa ttctctaaaa gttgatgatg ctgcaaaaat tgttggctgt
    12241 tggaaag
//
