LOCUS       NZ_CP035937            18761 bp    DNA     circular CON 22-APR-2024
DEFINITION  Acinetobacter cumulans strain WCHAc060092 plasmid p2_060092,
            complete sequence.
ACCESSION   NZ_CP035937
VERSION     NZ_CP035937.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN08772519
            Assembly: GCF_003024525.3
KEYWORDS    RefSeq.
SOURCE      Acinetobacter cumulans
  ORGANISM  Acinetobacter cumulans
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter.
REFERENCE   1  (bases 1 to 18761)
  AUTHORS   Qin,J., Maixnerova,M., Nemec,M., Feng,Y., Zhang,X., Nemec,A. and
            Zong,Z.
  TITLE     Acinetobacter cumulans sp. nov., isolated from hospital sewage and
            capable of acquisition of multiple antibiotic resistance genes
  JOURNAL   Syst Appl Microbiol 42 (3), 319-325 (2019)
   PUBMED   30808586
REFERENCE   2  (bases 1 to 18761)
  AUTHORS   Qin,J., Long,H., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2019) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
  REMARK    Sequence update by submitter
REFERENCE   3  (bases 1 to 18761)
  AUTHORS   Qin,J., Long,H., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-FEB-2019) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
REFERENCE   4  (bases 1 to 18761)
  AUTHORS   Qin,J., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAR-2018) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003024525.3-RS_2024_04_22
            Annotation Date                   :: 04/22/2024 04:03:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,515
            CDSs (total)                      :: 3,393
            Genes (coding)                    :: 3,310
            CDSs (with protein)               :: 3,310
            Genes (RNA)                       :: 122
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 96
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 26 of 83
            Pseudo Genes (incomplete)         :: 44 of 83
            Pseudo Genes (internal stop)      :: 25 of 83
            Pseudo Genes (multiple problems)  :: 11 of 83
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP035937.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..18761
                     /organism="Acinetobacter cumulans"
                     /mol_type="genomic DNA"
                     /strain="WCHAc060092"
                     /isolation_source="Sewage"
                     /type_material="type strain of Acinetobacter cumulans"
                     /db_xref="taxon:2136182"
                     /plasmid="p2_060092"
                     /geo_loc_name="China: Chengdu, Sichuan"
                     /collection_date="2018"
     gene            join(18267..18761,1..438)
                     /locus_tag="C9E88_RS17225"
                     /old_locus_tag="C9E88_016480"
     CDS             join(18267..18761,1..438)
                     /locus_tag="C9E88_RS17225"
                     /old_locus_tag="C9E88_016480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000742756.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element IS17 family transposase"
                     /protein_id="WP_099475569.1"
                     /translation="MKKPTHKIYRTTNWPAYNRALMSRGNIAIWFDPATQWYAPSKGKQ
                     GRNQTYSDAAIQCCLMIKSLFRLSLRMVTGFVQSLIKLCGLNWTAPDYSTLCRRQKHID
                     IAISYQKSSDGLHLLVDSTGMKFLGEGEWKRKKHGAEYRRQWRKLHIGIDAKTLQIRAI
                     QLTTNNVSDSQVLGDLLNQIPQDEQIDSVYTDGAYDTKQCRQVIADRQAHAVIPPRKNA
                     KPWKDTKSSSLERNELLRTVKHLGRTLWKKWSGYHRRSLVETKMHCIKLLGDKLMARSF
                     PSQVNEIHARVAVLNRFTELGRPLTQVTP"
                     /name="IS5-like element IS17 family transposase"
     ARG             479..1317
                     /name="APH(3'')-Ib"
                     /gene="APH(3'')-Ib"
                     /gene_name="APH(3'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="99.25"
                     /coverage="104.49"
     ARG             515..1314
                     /name="aph(3'')-Ib"
                     /gene="aph(3'')-Ib"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3'')-Ib"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            1317..2153
                     /locus_tag="C9E88_RS17235"
                     /old_locus_tag="C9E88_016490"
     CDS             1317..2153
                     /locus_tag="C9E88_RS17235"
                     /old_locus_tag="C9E88_016490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="WP_000480968.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
                     /name="aminoglycoside O-phosphotransferase APH(6)-Id"
     gene            2374..2730
                     /locus_tag="C9E88_RS17245"
                     /old_locus_tag="C9E88_016500"
     CDS             2374..2730
                     /locus_tag="C9E88_RS17245"
                     /old_locus_tag="C9E88_016500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005194067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004967307.1"
                     /translation="MWIVITTDLFNEWLEQQDESTQEKVLAALVVLQQQGPSLGRPLVD
                     TVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAIVLCIADKGSKKRFYKDMLDIADEQ
                     YQLHLTTLGDKSNG"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            2723..3025
                     /locus_tag="C9E88_RS17250"
                     /old_locus_tag="C9E88_016505"
     CDS             2723..3025
                     /locus_tag="C9E88_RS17250"
                     /old_locus_tag="C9E88_016505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_001140620.1"
                     /translation="MAKTLQELLASRSPESQARIQKMADELLLENQLHLIREELEISQK
                     ELAETLGIKQPSLSAIENRGNDLKISTMKKYVEAMGGKLRIDVELPTGKHIGFNV"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(3107..3382)
                     /locus_tag="C9E88_RS17255"
                     /old_locus_tag="C9E88_016510"
     CDS             complement(3107..3382)
                     /locus_tag="C9E88_RS17255"
                     /old_locus_tag="C9E88_016510"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004967304.1"
                     /translation="MKNKIEDLKSTIQELIELFEEQNLEHWAEMYKRILFYIDDDLEYA
                     KYKIQSTIGGMGSMDDIVLYRNGDPLIKENNRLDYLRSKLYVFCES"
                     /name="hypothetical protein"
     gene            3583..4194
                     /locus_tag="C9E88_RS17260"
                     /old_locus_tag="C9E88_016515"
     CDS             3583..4194
                     /locus_tag="C9E88_RS17260"
                     /old_locus_tag="C9E88_016515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004889724.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_015060246.1"
                     /translation="MIEMNTRIYLRASTKDQDAERALQILQDLNQNLNLGETIVYVENY
                     SGTKLDRPELNKLLSEANQGDTLLVESIDRLSRLTQRDFQELKRRIQEKGLRLVVADLP
                     TTYQMIQTSDSITHSILELINNMLIDLLATMARLDNEKRIERIKQGLARSGYKPTGKKA
                     NEAKHKRIKELLVVGNMTKEEIAKAVNCGVATVYRVAKVI"
                     /name="recombinase family protein"
     gene            complement(4384..5268)
                     /locus_tag="C9E88_RS17265"
                     /old_locus_tag="C9E88_016520"
     CDS             complement(4384..5268)
                     /locus_tag="C9E88_RS17265"
                     /old_locus_tag="C9E88_016520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(E) family macrolide 2'-phosphotransferase"
                     /protein_id="WP_000155092.1"
                     /translation="MTIQDIQSLAEAHGLLLTDKMNFNEMGIDFKVVFALDTKGQQWLL
                     RIPRRDGMREQIKKEKRILELVKKHLSVEVPDWRISSTELVAYPILKDNPVLNLDAETY
                     EIIWNMDKDSPKYITSLAKTLFEIHSIPEKEVRENDLKIMKPSDLRPEIANNLQLVKSE
                     IGISEQLETRYRKWLDNDVLWADFTQFIHGDLYAGHVLASKDGAVSGVIDWSTAHIDDP
                     AIDFAGHVTLFGEESLKTLIIEYEKLGGKVWNKLYEQTLERAAASPLMYGLFALETQNE
                     SLIVGAKAQLGVI"
                     /name="Mph(E) family macrolide 2'-phosphotransferase"
     gene            complement(5324..6799)
                     /gene="msr(E)"
                     /locus_tag="C9E88_RS17270"
                     /old_locus_tag="C9E88_016525"
     ARG             complement(5328..6799)
                     /name="msr(E)"
                     /gene="msr(E)"
                     /gene_name="ABC-F type ribosomal protection protein Msr(E)"
                     /tool="amrfinderplus"
                     /drug_class="MACROLIDE/STREPTOGRAMIN"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(7292..7564)
                     /gene="nadS"
                     /locus_tag="C9E88_RS17275"
                     /old_locus_tag="C9E88_016530"
     CDS             complement(7292..7564)
                     /gene="nadS"
                     /locus_tag="C9E88_RS17275"
                     /old_locus_tag="C9E88_016530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000369784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="WP_000369781.1"
                     /translation="MDNNLFDDLVASIKEAGAIKRKEVKASRVTELELPDIKEVREKTG
                     LSQNEFAARLHISPRTLQNWEQGRRYPTGPAATLIRILDAHPSLI"
                     /name="nadS"
     gene            complement(7557..7877)
                     /locus_tag="C9E88_RS17280"
                     /old_locus_tag="C9E88_016535"
     CDS             complement(7557..7877)
                     /locus_tag="C9E88_RS17280"
                     /old_locus_tag="C9E88_016535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_000897307.1"
                     /translation="MLFIETSIFTKQIKDLVSDEEYRQLQQDLLVQPDRGDLIKNGGGI
                     RKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAYPKSVKDNLTDKETAILHQLVKEQF
                     HG"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(8036..8677)
                     /locus_tag="C9E88_RS17285"
                     /old_locus_tag="C9E88_016540"
     CDS             complement(8036..8677)
                     /locus_tag="C9E88_RS17285"
                     /old_locus_tag="C9E88_016540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016927662.1"
                     /GO_process="GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="WP_004787586.1"
                     /translation="MSKKDIAPQRLTREIVLRTALDMLNEEGIDSITTRKLAQRLGIKS
                     PTLYWHFKNKSLLMEAMAETIINEHHLVSLPIDGMTWQDWLLANSVSFRRALLAYRDGA
                     RLHARTSPSQGHFNTIEAQVALLSHAGFSPVEAVALLMTLGRFIVGWVLEEQQEEIRSD
                     PPFEADPTIYPLMLQGVNTLQNMNADDIFENGIRMVIIGAERQLDIKMQT"
                     /name="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
     gene            8743..9930
                     /gene="tet(39)"
                     /locus_tag="C9E88_RS17290"
                     /old_locus_tag="C9E88_016545"
     ARG             8744..9927
                     /name="tet(39)"
                     /gene="tet(39)"
                     /gene_name="tetracycline efflux MFS transporter Tet(39)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(10154..10297)
                     /locus_tag="C9E88_RS17650"
     CDS             complement(10154..10297)
                     /locus_tag="C9E88_RS17650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_167514224.1"
                     /translation="MPKLKDIALGIIVAPLLIPIMLIASYQDKKELKKELDKRQKEKDQ
                     AS"
                     /name="hypothetical protein"
     gene            complement(10310..10864)
                     /locus_tag="C9E88_RS17295"
                     /old_locus_tag="C9E88_016550"
     CDS             complement(10310..10864)
                     /locus_tag="C9E88_RS17295"
                     /old_locus_tag="C9E88_016550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004282116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_068975321.1"
                     /translation="MAKLSLSEVSNRFNVSRSTLYRAIKEGRISRSADGLFDIAEVIRC
                     FGEPIKKHEQKQEVDQSKDDADLRQLVDFMKKEIESYKDREKRYLDQIDRFQLLLGHKE
                     SEEKMSHDTPVTHSNDAPCDNNSGISETIDNNRDSEIKTSHETLVTRPNETPKHVPNTH
                     YETKKKRGLFGRVLNAVFDND"
                     /name="plasmid replication DNA-binding protein"
     gene            complement(10868..11812)
                     /gene="repM"
                     /locus_tag="C9E88_RS17300"
                     /old_locus_tag="C9E88_016555"
     CDS             complement(10868..11812)
                     /gene="repM"
                     /locus_tag="C9E88_RS17300"
                     /old_locus_tag="C9E88_016555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798284.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_068912167.1"
                     /translation="MKNDLVVKDNALINASYNLEVTEQRLILLSIIRARETGQGISSDS
                     KLEIHASDYASRFDVTKEAAYNALKNAVNNLFERKFSFKEIHKDTNKEIVVKSRWVSRI
                     AYVDDLATLEVTFAPDVVPLITRLEKHFTSYQLKQVAQLTSKYAIRLYEFLIAWRDVGK
                     TPVISLSEFREKLGLEMNEYQKMVNFKSRVLEPAIKQINELTDIHVKYEQYKTGRSISG
                     LSFTFKHKKIDSIPSERDPNTVDLFTQMTDAQRHMFANKLSELPEMGRYSQGTESYPQF
                     AIRIAEMLHDPEKIKELYPYLKKVGYMPLNKRI"
                     /name="repM"
     gene            complement(12446..14200)
                     /gene="mobQ"
                     /locus_tag="C9E88_RS17305"
                     /old_locus_tag="C9E88_016565"
     CDS             complement(12446..14200)
                     /gene="mobQ"
                     /locus_tag="C9E88_RS17305"
                     /old_locus_tag="C9E88_016565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002011654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_227866002.1"
                     /translation="MAIFHLNESNISRSDGRSAVACAAYRACEKLEDLTFGKTQDYTKK
                     KGLEYKNIYAPEHTNTKLLDRQTLWNTVEKKEFNANGSMKANARLAKEYICALPHELTD
                     AERIKIVDDFCRDFVNKHNVIVDACIHAPHEHNDETNNKNYHVHMMFTTRLINEKGELG
                     KKQRIFNDHGPEILKDSRATFANVVNTVLENAGLDERIDHRSYKDQGLDFLEPTHHEGH
                     EATALRRQYDEEQKRPLEERNTEIVLPRIALENDAIKAKNLDAAREYQQIIKGLDQEII
                     VPSRLEDQITQLENELQLTEAEEKELLAELVNLNLEEERLQEQQVQQIDNAYDDFIRCQ
                     DIYAEFANQFYTIQSNAADNQKQIESNLTKTKRWLAENKSDFYLHTNNLFYDSYHHTYR
                     DIKKPDFYATEKSVEQAKNENWREYATEVEQLAKEYDIENVVQRLGQCSEILENNGIER
                     PTIKPTFWQKLKREYVHSFDTLHDFNDDMSPLLKAKRADDLKIEQERMQQVRQAEVDRQ
                     RRIENDRRESEFREQLRKEREQKEQRYEQERHEREHLAFLKRQELEKQQKNEPKKPENE
                     NNNDYRPW"
                     /name="mobQ"
     gene            14459..14839
                     /locus_tag="C9E88_RS17310"
                     /old_locus_tag="C9E88_016570"
     CDS             14459..14839
                     /locus_tag="C9E88_RS17310"
                     /old_locus_tag="C9E88_016570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_106986733.1"
                     /translation="MSDIQEKKDAVLKKLTKAQQKKLSLQEQIEYLKKEQEQILEQAKQ
                     KQNELKLKQQKLLSEAKAQERKNETRRKILLGAWVLNKFKNDESFKHQWADFEQFLSAE
                     SKTEENRQKDKELFNNLFKGKF"
                     /name="hypothetical protein"
     gene            complement(14903..15367)
                     /locus_tag="C9E88_RS17315"
                     /old_locus_tag="C9E88_016575"
     CDS             complement(14903..15367)
                     /locus_tag="C9E88_RS17315"
                     /old_locus_tag="C9E88_016575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005093372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004968108.1"
                     /translation="MLPMLASQLQQETGWRVTSCEILLDQSHTNTDESFGNIEITSKHG
                     ITLFFGFTSSTNKKIEMDEIRIFNAENIEVCIDENDANNVFAAAYDIVDQTNWRKIAIN
                     AKYEKQSRKVKSISFNRESEADLLHFVENQKDFSNWVKSKILEEVSKSHG"
                     /name="hypothetical protein"
     gene            complement(15585..>15677)
                     /locus_tag="C9E88_RS17890"
                     /pseudo=""
     CDS             complement(15585..>15677)
                     /locus_tag="C9E88_RS17890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004186876.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3761 domain-containing protein"
                     /name="DUF3761 domain-containing protein"
     gene            15749..16968
                     /locus_tag="C9E88_RS17325"
                     /old_locus_tag="C9E88_016585"
     CDS             join(15749..16025,16025..16968)
                     /locus_tag="C9E88_RS17325"
                     /old_locus_tag="C9E88_016585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612089.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="WP_100249712.1"
                     /translation="MTKLKYAPEIRERAVQLLIESEKDYPSNWAAITAIAPKIGCTPET
                     LRVWYQKYLDKLNPVKVQQLSDQERIKQLERENKELQRANEILRKAAGFFRPGGTRPPT
                     QIMVDFIHNNKELYGVEAICRILPIAPSTYYRTLDLCENPEHRAKRDLHDLHHAEQIKR
                     IWKESSGRYGVRKVWQKLKREGYIIARCTVARLMQKLGIQGVWRGKNKQTTHSRDDQKR
                     ADDLVKRNFSADHPDQLWVADFTYIQTNSGWVYTAFIIDVFSRAIVGWKVSTRMNTDMV
                     LDALEQALHDRGMPKNVIHHSDRGVQYLSIRYTNRLEAANLRASVGTTGDSYDNALAET
                     VNGLYKTEVIEYLKADWQGLADVQLATLNWVDWFNKERVHSALGYVPPFDFEAMYYDKI
                     NPLGQVA"
                     /name="IS3 family transposase"
     misc_feature    16024..16140
                     /locus_tag="C9E88_RS17325"
                     /old_locus_tag="C9E88_016585"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01497"
     gene            17103..17918
                     /gene="sul2"
                     /locus_tag="C9E88_RS17330"
                     /old_locus_tag="C9E88_016590"
     ARG             17104..17915
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            18005..>18217
                     /gene="glmM"
                     /locus_tag="C9E88_RS17335"
                     /old_locus_tag="C9E88_016595"
                     /pseudo=""
     CDS             18005..>18217
                     /gene="glmM"
                     /locus_tag="C9E88_RS17335"
                     /old_locus_tag="C9E88_016595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008891390.1"
                     /note="catalyzes the conversion of glucosamine-6-phosphate
                     to glucosamine-1-phosphate; incomplete; partial in the
                     middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /name="glmM"
     ARG             complement(4388..5268)
                     /name="mph(E)"
                     /gene="mph(E)"
                     /gene_name="Mph(E) family macrolide 2'-phosphotransferase"
                     /tool="amrfinderplus"
                     /drug_class="MACROLIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             1318..2150
                     /name="aph(6)-Id"
                     /gene="aph(6)-Id"
                     /gene_name="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(5325..6799)
                     /name="msrE"
                     /gene="msrE"
                     /gene_name="msr-type ABC-F protein"
                     /tool="rgi"
                     /drug_class="macrolide antibiotic; streptogramin
                     antibiotic"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(4385..5268)
                     /name="mphE"
                     /gene="mphE"
                     /gene_name="macrolide phosphotransferase (MPH)"
                     /tool="rgi"
                     /drug_class="macrolide antibiotic"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(10858..11812)
                     /name="rep_cluster_1154"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.507"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(12450..13978)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.216"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          16067..16965
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 acaaccaata atgtcagtga ttcacaggtg cttggtgatt tacttaatca gattccacaa
       61 gatgagcaga ttgactctgt ttataccgat ggagcttatg acaccaagca atgccgtcag
      121 gtcattgcag atcggcaagc gcatgcggtg attccaccta gaaaaaatgc gaaaccatgg
      181 aaagatacaa agagtagctc gctagagcga aatgaattac ttcgaacagt taaacattta
      241 ggcaggacat tatggaaaaa atggtcaggc tatcatcgcc gcagtttggt ggaaaccaag
      301 atgcattgca tcaaattatt aggcgataaa ttaatggcaa gaagctttcc tagtcaggtg
      361 aatgagatcc atgcacgtgt agcagtcctt aacagattta cggaattagg tcgcccactt
      421 acccaagtta cgccttaaat ttggatcaat taggggcgct ttgcatttca aatctttgtg
      481 caacaaagcc tcagaaaact gaaggaacct ccattgaatc gaactaatat tttttttggt
      541 gaatcgcatt ctgactggtt gcctgtcaga ggcggagaat ctggtgattt tgtttttcga
      601 cgtggtgacg ggcatgcctt cgcgaaaatc gcacctgctt cccgccgcgg tgagctcgct
      661 ggagagcgtg accgcctcat ttggctcaaa ggtcgaggtg tggcttgccc cgaggtcatc
      721 aactggcagg aggaacagga gggtgcatgc ttggtgataa cggcaattcc gggagtaccg
      781 gcggctgatc tgtctggagc ggatttgctc aaagcgtggc cgtcaatggg gcagcaactt
      841 ggcgctgttc acagcctatc ggttgatcaa tgtccgtttg agcgcaggct gtcgcgaatg
      901 ttcggacgcg ccgttgatgt ggtgtcccgc aatgccgtca atcccgactt cttaccggac
      961 gaggacaaga gtacgccgca gctcgatctt ttggctcgtg tcgaacgaga gctaccggtg
     1021 cggctcgacc aagagcgcac cgatatggtt gtttgccatg gtgatccctg catgccgaac
     1081 ttcatggtgg accctaaaac tcttcaatgc acgggtctga tcgaccttgg gcggctcgga
     1141 acagcagatc gctatgccga tttggcactc atgattgcta acgccgaaga gaactgggca
     1201 gcgccagatg aagcagagcg cgccttcgct gtcctattca atgtattggg gatcgaagcc
     1261 cccgaccgcg aacgccttgc cttctatctg cgattggacc ctctgacttg gggttgatgt
     1321 tcatgccgcc tgtttttcct gctcattggc acgtttcgca acctgttctc attgcggaca
     1381 ccttttccag cctcgtttgg aaagtttcat tgccagacgg gactcctgca atcgtcaagg
     1441 gattgaaacc tatagaagac attgctgatg aactgcgcgg ggccgactat ctggtatggc
     1501 gcaatgggag gggagcagtc cggttgctcg gtcgtgagaa caatctgatg ttgctcgaat
     1561 atgccgggga gcgaatgctc tctcacatcg ttgccgagca cggcgactac caggcgaccg
     1621 aaattgcagc ggaactaatg gcgaagctgt atgccgcatc tgaggaaccc ctgccttctg
     1681 cccttctccc gatccgggat cgctttgcag ctttgtttca gcgggcgcgc gatgatcaaa
     1741 acgcaggttg tcaaactgac tacgtccacg cggcgattat agccgatcaa atgatgagca
     1801 atgcctcgga actgcgtggg ctacatggcg atctgcatca tgaaaacatc atgttctcca
     1861 gtcgcggctg gctggtgata gatcccgtcg gtctggtcgg tgaagtgggc tttggcgccg
     1921 ccaatatgtt ctacgatccg gctgacagag acgacctttg tctcgatcct agacgcattg
     1981 cacagatggc ggacgcattc tctcgtgcgc tggacgtcga tccgcgtcgc ctgctcgacc
     2041 aggcgtacgc ttatgggtgc ctttccgcag cttggaacgc ggatggagaa gaggagcaac
     2101 gcgatctagc tatcgcggcc gcgatcaagc aggtgcgaca gacgtcatac tagatatcaa
     2161 gcgacttctc ctatcccctg ggaacacatc aatctcaccg gagaatatcg ctggccaaag
     2221 ccttagcgta ggattccgcc ccttcccgca aacgacccca atatgaaaaa ccccggcagg
     2281 atgctggggt tttttattgc ctatcatttc tcatttgcaa aacaagcata atgctcctaa
     2341 aatatagact ataatctata ttttggttgt ggtatgtgga tagtcattac gacagatcta
     2401 tttaatgaat ggcttgaaca gcaagacgaa tcaacacaag agaaggttct ggctgcccta
     2461 gttgttctac agcagcaagg accaagttta ggccgccctt tagtagatac tgtctatgac
     2521 tctaaattta ccaacatgaa agagcttcgt gttcaacatc gaggtaaacc actgagagct
     2581 ttcttcgcat ttgatccttt acgacaggct attgttctat gtattgctga taaaggcagt
     2641 aaaaagcgtt tttataaaga catgctggat attgcagatg aacaatacca acttcatctc
     2701 accaccctag gagataaatc taatggctaa gactctgcaa gaattgttag ctagccgctc
     2761 tccagagagc caagcccgta ttcaaaaaat ggcagatgaa ttactgctag aaaatcagct
     2821 tcatcttatt cgtgaagaac ttgaaatctc tcaaaaggaa cttgctgaaa ctttgggaat
     2881 aaaacagcca tcgctttcag caatagaaaa tcgtggcaat gatcttaaga tttcaaccat
     2941 gaaaaagtat gttgaggcaa tgggcgggaa gctccgtatt gatgtagagc ttccaacagg
     3001 aaaacatata ggattcaatg tttaacaata aaaatgggag ctaaagactc ccatttttag
     3061 attcgtataa tcgccattat gttaaattag cgatattaag aataatttag ctttcgcaaa
     3121 aaacatataa tttacttctt aagtaatcca aacgattgtt ttctttgata agtggatcac
     3181 cattgcgata taaaacgata tcatccattg atcccattcc tccaatggta ctttgtattt
     3241 tatattttgc gtattctaga tcatcatcaa tatagaacag tattctttta tacatttctg
     3301 cccaatgttc taaattctgt tcttcaaaca attctattag ttcttgaatt gttgatttta
     3361 aatcttcaat tttatttttc ataaaagtat tttttaagat tctctaaaat taacataata
     3421 cacgttatac gaattcccaa ggcatggctt ttagtcatgc tttttttatc aaaagactat
     3481 acctgaatga tagcgagcca aatcgcatac aatatctatc attagactta atgcaataat
     3541 taaatgatag tattatcatt aatctaatag aattaagatt aaatgataga aatgaataca
     3601 cgtatttatc tacgagcttc aactaaagat caagatgcag aaagggcttt gcagattctt
     3661 caggatctaa accaaaactt gaatttgggt gaaaccattg tgtacgtgga aaactatagt
     3721 ggtacgaagt tagaccgacc tgaattgaat aagctactgt cagaagcaaa tcaaggtgac
     3781 acattgttag ttgaaagtat tgaccgctta tcacgcctaa cccaacgaga ttttcaagag
     3841 ctgaagcgca ggatccagga gaagggactt cgtttagtcg tagcagatct tcctactacc
     3901 taccaaatga ttcaaaccag tgacagcatt actcattcaa tcttggaact catcaacaat
     3961 atgttgattg atcttctggc aacaatggcc cgcctagaca atgagaaacg tatagaacgt
     4021 attaagcagg gcctggcacg ttcgggttac aaaccaacag gcaagaaggc aaatgaggct
     4081 aaacataaac gaataaaaga attgctagta gttggcaata tgactaagga agaaattgcc
     4141 aaagcagtga attgtggagt tgcaactgtc tatcgagttg ctaaagttat ctaaaagagg
     4201 cttatctcaa cgcgccccca atggggcacc gctgcattca ggttacttca cataagaaat
     4261 tttatgttaa atgattgtta gaaatgcccc ttgatcgagg cattttctat aaagaatact
     4321 aaaaactaat taaagccaac caagtttcaa aacaaggcag ttctcagcaa tcatattttt
     4381 aaattatata actcccaact gagcttttgc tccaacgata aggctttcat tttgagtttc
     4441 taaggcaaat aaaccataca tcaaaggaga ggccgctgct ctttctaaag tctgttcata
     4501 tagtttattc caaactttac cccctagttt ttcatactcg atgattagag ttttgaggct
     4561 ttcttctcca aacaaagtta catgcccagc aaaatcaatc gctgggtcat ctatatgggc
     4621 tgttgaccaa tcaataacgc ctgaaacagc tccatccttt gaagctagta catgcccagc
     4681 atataaatcg ccatgtataa attgggtgaa atctgcccat agaacatcat tatccaacca
     4741 ttttctgtag cgggtttcca attgctcact tataccaatt tcagatttta ctaactgcaa
     4801 attgtttgct atttcaggtc ttaaatctga aggtttcata attttcaaat cattttcccg
     4861 aacttctttt tcaggaatac tatggatttc aaataaggtt tttgccaaag atgttatgta
     4921 tttcgggcta tctttgtcca tattccaaat tatttcatag gtttcagcat ccaaatttaa
     4981 aacaggatta tctttaagta tgggataagc cactaattct gtagatgaaa ttctccaatc
     5041 aggaacctct acagaaagat gtttttttac caattctaaa atgcgttttt ctttcttgat
     5101 ttgttccctc atgccatcac gacgaggaat acgcagcaac cattgttgcc cctttgtatc
     5161 aagagcaaaa acgaccttaa aatcaatgcc catttcattg aaattcattt tgtccgtaag
     5221 caacaagccg tgtgcttcag caagtgattg aatatcttga attgtcattt ttaatttcct
     5281 ttaaagagtt caataattaa tgttcggatt agattggcta tcattaacaa tctctctcaa
     5341 aagtcttgat gattttgtgg tctttgatct cgtagataat gtcagcaata ttatcgacca
     5401 attgcttgtc atgagatacg aagataatag ttcctgcata ggacttcatc attgtttcta
     5461 atgcggcaat actttttagg tcaagatagt ttcctggttc atccataagc aaaatattat
     5521 attttcctaa aagcattttg gataaaagca gtttgatgat ttcacctccc gataagtcgg
     5581 ataagttttt ttgaatatca ttcgctccga tccccattga agccaatact gcacgaattt
     5641 ccgcaactgt gtactcgcac tcttcctgca taaaggagag cacagattta tgcgtgttaa
     5701 atttatatcc tgtttgtgta aagtagccaa tttcagcttt tggagatatg gttaatccat
     5761 cagcacgttc tgatatcatt tttaacaagg acgttttccc tgttccattc gatccagtta
     5821 tagcgacttt agcgccaagc ggtattataa agttagcgtc atcaaagata gtacggctac
     5881 caaattttaa gctcagacca tctgccgtaa tcgggaactt attgtgcagt tctagggctg
     5941 aactttgacg aaaacgaata gaacgcaaat gctctggtgc ttgaatatct tctaatgcag
     6001 ccaaacgctt ttccatactc ttagctgcct gatacagttt tctttgcttg gtgccagtca
     6061 tttttgcatg cccaagtcgt ccagcacttt cggtagagtt tttggatttt tctccttttt
     6121 tcttattgtc taatcgatta gcttgctggc gtttttcttg cacagcagat tctaatcgct
     6181 cccgttcctt catcatcagc tcatattcta cggcttggtg ttgtcgctct tcttcttttt
     6241 gacgcaagta atccgagtaa ccaccccaat attccgtaat tttaccgtct tttaactccc
     6301 atatcttgtc tacaaccata tcaagaaaat atcggtcatg actgataaca agtaatgctc
     6361 catcaaatgc tttaagttga ccaataagta gatctattcc attgagatca aggtggctgg
     6421 ttggttcatc cgctagaatg ccatgtactt gttgggaaaa tgcggcagca atttttgcac
     6481 gagtttcctc tccgccactc attgtgtcgt tttgtacatt ggaaacacca aggcgagata
     6541 acattgcccg gtcttcgacc gtttctattt cgattccgcc cagttggctg atatgtgcaa
     6601 aatcaccaaa acgctgtaat gtcgcttcgg ctaaaacaat ttcgccatta agtactttga
     6661 gtaaactact ctttcctgct ccgttatcac ccacaagacc aatacggtca taagagtgaa
     6721 tttccaattc atcaatatcc aaaacatcac gcccagcata atccaagcgt atgtttctcg
     6781 ctttaataat taaactcatt tttatttact cctgtttagc tcttgaaatt ttttatgcag
     6841 caaacaggat ttaggtgaaa acaaaagcta gcatcacagt gtcctcccaa aaaaaaagct
     6901 attcatccac agggtggaca aatagctagt caattaagtt ataactggaa actatgcact
     6961 aaaagcatac ttataaatta agcatacttt tactttatat atccgcatat attcttaaag
     7021 acacaacaaa agcccaccat tataaaatag tgtcactatg caaatagttg tgtcttaacg
     7081 aatgcggata gaatgcataa acttacctaa aaaataaaat tcagttttat gataacttta
     7141 ctgttaaaag aagtctagac aaccttgttt tatgcttgga tataaggctt attttaaaat
     7201 aatagtagtg aagattttct aaaattaaca taatacacct tatacgaaat aaaaatggga
     7261 gccttaagct cccatttttt aagtaatttt ttcaaataag gcttgggtga gcatctaaaa
     7321 ttcgaatcaa tgtagctgct ggtccagttg gatagcgtcg tccctgttcc caattttgca
     7381 gggtacgggg gctaatatgt agacgtgcag caaattcatt ttggctcaag ccagtttttt
     7441 cacgtacttc tttaatatca ggcaattcga gttctgtaac tcggcttgct ttaacttctt
     7501 tacgtttgat agctccagct tctttgattg aagcaaccaa gtcatcaaat aagttgttat
     7561 ccatgaaatt gctccttcac gagttggtgc agaatggcgg tttccttatc tgttaaatta
     7621 tcttttactg attttggata agccactaag aaaaagatct gatcatcctc ggtgacccaa
     7681 taataaatca cccgtatccc accacttttc cctttgttac cttgagcaca acgtactttg
     7741 cgaataccac cgccattctt gattaggtca cctctatcag gctgtaccaa aagatcttgc
     7801 tgaagttgac gatattcctc atcagataca aggtctttaa tttgcttggt aaagatgctg
     7861 gtttcaataa ataacatgga ctctttatac gtcaatggcg tatttgaatt ttagcagttt
     7921 ccataacaaa aaacgagagc tggtagatct cgtttcgcat aacagccatt atgttaaata
     7981 attaaggata aaagctttat tactaaatag aacagttgct taaagcagta tagagttaag
     8041 tttgcatttt aatatcaagt tgtctttcag caccgataat caccatacga atcccatttt
     8101 caaaaatgtc atccgcattc atattttgta aggtattcac tccttggagc atgagtggat
     8161 atatagttgg atcggcttcg aatggtgggt ctgatcttat ttcttcttgc tgctcctcaa
     8221 ggacccaccc aacaataaac cttcctaatg tcataagtaa tgcaactgct tcaacaggag
     8281 aaaacccagc atgagataac agcgcaactt gggcctcaat cgtgttgaaa tgtccttgac
     8341 tggggctagt tcttgcatgt agacgagcac catcacggta agctagtagt gctcgacgaa
     8401 agcttacaga attagcgagc aaccaatctt gccatgtcat cccatcgatg ggtaaagaaa
     8461 ctaaatggtg ttcatttata atggtttccg ccattgcttc cattaacaag gatttattct
     8521 tgaaatgcca atatagagtt ggagatttaa tacccaatcg ttgagctagc ttgcgtgtgg
     8581 ttattgaatc tatgccttct tcattcaaca tatccaatgc ggtacgcagg actatttcac
     8641 gtgtcagacg ttgaggtgca atatcttttt tactcataaa tctatcactg atagggagtg
     8701 tgtgagagaa tattaattct atcattgcta gagaaggcat ttgtgaagaa atcattgagc
     8761 gtgattttaa tcactatatt tctggatgct gttgggattg gtttaattat gccgatcttg
     8821 cctgaattat tacggtcatt ggctggagct gaagcaggcg gtgttcacta tggtgcttta
     8881 ttagctgtgt atgctctgat gcagttcatt tttgcaccta tccttggagc gttgagtgac
     8941 cgatttggac gtcgacctgt attaattatt tcaattgctg gtgcaacggc tgattatctc
     9001 ctaatggctg ctgctccttc tctattgtgg ctatatattg gtcgtatttt tgcgggaatt
     9061 acaggtgcca acatggctgt tgcaacagct tatgtttcag atattactcc agcccatgag
     9121 cgtgcaaaaa ggtttggtct ccttggagct gtctttggta ttgggtttat agcgggtccg
     9181 gtaataggtg gagttttggg tgaatggaac ttacatgcac cgttctttgc tgctgctttt
     9241 atgaatggga ttaatttaat aatgacagca gtcttattaa aagaatcaaa acacagcaat
     9301 aaaatgactg agaaggttca ggagcaatca atattaaaga aattatccta tttgatcact
     9361 caacctaata tggctccatt gcttggtatc tttttaatta tcacattggt ttcacaagtc
     9421 cccgcaactt tatgggttat ctatgggcag gatcgttatg gctggagtat atttattgca
     9481 ggtgtttccc ttgctagtta tggaatatgc cattctattg cacaggcttt tgctatcgcc
     9541 cctatggtaa agaggtttgg agagaaaaat acgttgttat gtggaatagc ttgcgatgca
     9601 attggtttac ttcttttatc tattgctgtt gaagaatggg tgccttttgc gttgttacca
     9661 ttgtttgccc ttggtggagt agccgttcct gctttgcaag caatgatgtc cagaggtatt
     9721 agtgatgaaa gacaaggtga attacaaggg ctattaagca gttttaatag tctgggggct
     9781 ataattggtc ctgtattagt tactagcctc tattttatga ctcaggcatc agctcctgga
     9841 atggtatggg cattagctgc aatactttat gtaatcaccc tacccttatt gcttaagtat
     9901 cgcctgaata aatattctgg agttccataa aatatttaat ttattggttt aaaaactaat
     9961 ggattctata gtttatctaa aattaacata atacacgtta tacgaaacgt gatataattt
    10021 tccttgaaat tcatatactt atttcaaagt ttatagcccg aacatcactg ttcgggcttt
    10081 ttttatgatt tatcacgttc cacgcctatt atttaagcaa tgtttcacga ttttgattag
    10141 ctcaattgaa taatcaggat gcctggtctt tttctttttg gcgtttatcc agctcttttt
    10201 tcagctcctt tttatcctgg tacgatgcga tcagcatgat cgggatcaac agcggggcca
    10261 caataatgcc tagggcaatg tctttcagtt tcggcatgat ctttctccct tagtcattat
    10321 caaaaacagc attcagtaca cggccaaata agccacgttt tttctttgtc tcataatgcg
    10381 tatttggtac gtgttttggt gtttcattag ggcgtgtcac caatgtctca tgggatgttt
    10441 taatttcact atctctatta ttatcaatag tttcacttat tccactattg ttgtcgcatg
    10501 gtgcatcatt ggaatgtgtc actggtgtat catgagacat tttttcttca gactctttat
    10561 gccctaatag caattgaaag cggtctattt gatctaaata gcgtttctca cgatctttgt
    10621 atgactcaat ttcctttttc ataaaatcaa caagctgacg taaatcagca tcatctttag
    10681 actgatccac ttcttgtttt tgttcatgct ttttgatcgg ctctccaaag catcggatga
    10741 cttctgcaat atcaaaaaga ccatcagcgc ttcgagatat acgcccttct tttatggccc
    10801 tatatagcgt agaacgactg acgttaaagc ggttagatac ttcacttagt gataatttag
    10861 ccatttacta aatcctttta tttaatggca tatatcccac tttttttagg tatggatata
    10921 gttctttgat tttttcagga tcgtgaagca tctcagcaat acgaatagca aattgtggat
    10981 agctttcagt tccctgggaa taccgaccca tttccggtag ctctgaaagt ttattggcaa
    11041 acatgtggcg ttgtgcatcg gtcatttggg taaagagatc taccgtgttt ggatctcttt
    11101 ctgaaggtat cgaatcaatt tttttatgct taaaagtgaa tgataagcct gagatagatc
    11161 ttcctgtttt gtactgttcg tattttacat gtatatcagt cagctcattg atttgcttta
    11221 tggctggttc taaaacacga cttttaaaat taaccatttt ttgatattca ttcatctcaa
    11281 gtcctaattt ttctctgaac tcagataaag aaataacagg tgtttttcct acgtctctcc
    11341 aagcaattag aaactcatat agacgaatag catatttgct agttagttgc gcaacctgtt
    11401 taagttgata gctcgtaaag tgcttttcca atcttgtaat aagaggaaca acatctggtg
    11461 caaaggtaac ttctaatgta gcaaggtcat caacatatgc aattcggcta acccagcggg
    11521 atttgactac aatttctttg tttgtatctt tatgaatttc cttaaaagag aactttcttt
    11581 caaaaagatt attaactgca ttttttagag cgttatacgc agcttctttg gtgacatcaa
    11641 agcgagaagc ataatcagaa gcatggattt ctaatttact gtcggatgag atcccttggc
    11701 ctgtttctct tgctcttatt attgataaaa ggataagccg ttgctcggtt acttcgagat
    11761 tataactggc attaattaag gcattatctt ttactactaa gtcatttttc ataagactgc
    11821 ataggttata agtgttatta agactacatt agcattcgat gtaatcaaat gcaaagaaat
    11881 tgtagtctta tgacaaagaa attgtagtct tatgacaaag aaattgtagt cttatgacaa
    11941 agaaattgta gtcttatcca ccttgaaagc cttgcaaaca aaggttttca gagagtctaa
    12001 aagcattaaa aactttaaaa ataattaaaa gccaaggtaa ggaaaaattg ccctcggttt
    12061 tcgctacgct caaactctat tgaacttcgc tttcgctcag ttcgtagggg caatttttgg
    12121 cttaaaattt cgtgactttt aaaaaagaaa agcaactagg aattcgcttc gctcatagaa
    12181 ctttttcact cgcttcgctc gacataaagc catcgtaatc agttcgcacc cattcgggat
    12241 gctctgattc tgagttcttg atacacaggg gagcctgtaa tcagggaaaa acaggtgaaa
    12301 aatttagatc cctgtagttc gctcgtagac actcgctaaa tgctctcatt tgcgatttaa
    12361 gagttttttc ttaactcaac tataaatcta ctgtggttta gcaaaacatc gctaaaaacc
    12421 ctattttttt gcgtttaaac agcattcacc aaggtcggta atcgttgtta ttttcatttt
    12481 ctggtttttt aggctcgttt ttctgctgtt tttccaattc ttggcgtttt aaaaatgcca
    12541 aatgttctcg ttcgtgtctt tcttgttcat agcgttgttc tttttgctct cgttctttac
    12601 gtaattgctc tctaaattca ctttctctgc gatcattttc aattcttcgt tggcgatcta
    12661 cttccgcttg gcgtacttgc tgcattcttt cttgttcaat tttgagatca tctgcccgtt
    12721 ttgctttaag gaggggactc atatcatcat taaaatcatg tagggtatcg aatgaatgaa
    12781 cgtactctcg ttttagcttc tgccaaaacg tgggttttat cgttggtcgt tcaatcccat
    12841 tattttccag tatctctgag cattgaccta agcgttggac gacattctca atatcgtatt
    12901 ctttagcaag ttgttcgacc tcggttgcgt attctctcca gttctcattt tttgcctgtt
    12961 ctactgattt ttcagtcgca taaaaatcag gtttttttat atctctgtaa gtatgatggt
    13021 aagagtcata aaacaaatta ttggtatgta ggtaaaaatc gcttttgttt tctgctaacc
    13081 atcgttttgt cttggtgaga tttgattcaa tctgcttttg attatcagca gcattgcttt
    13141 gaatggtgta aaactgattg gcaaattctg cataaatatc ttggcaccgg atgaagtcat
    13201 catatgcgtt gtcgatctgt tgaacctgtt gttcttgcag gcgttcttct tctaggttga
    13261 gatttactaa ttctgctaaa agctcttttt cttctgcttc ggttaactga agttcgtttt
    13321 ccagctgggt aatctgatct tctaatcgac ttggaacaat aatttcttga tcaaggccct
    13381 tgatgatctg ttggtattct cgtgcagcat ctaaattttt ggctttgatt gcatcatttt
    13441 caagtgcaat tcttggcagc acaatttcag tgttacgttc ttcaagcggt cttttttgtt
    13501 cctcgtcata ttgtctgcga agtgctgtag cttcatgccc ttcatgatga gtgggttcaa
    13561 gaaagtctaa gccctgatcc ttgtaactcc ggtggtcgat gcgttcgtct agtccggcat
    13621 tttccaatac tgtgtttaca acattggcaa aagttgctcg gctgtccttc aggatctcgg
    13681 gaccatggtc gttgaagatt ctttgtttct ttcctaattc gcctttttca ttgatgagtc
    13741 tggtcgtgaa catcatgtgt acgtgatagt ttttattatt ggtttcatcg ttatgttcat
    13801 gtggtgcatg gatgcaggca tcgacaataa cattgtgctt gttcacaaag tctcggcaaa
    13861 agtcatcaac gatttttatg cgttctgcat ctgttaattc atgtggtaaa gcacagatat
    13921 attctttggc gagtcgtgca ttggctttca ttgagccatt ggcattgaat tctttttttt
    13981 ctactgtatt ccaaagtgtt tgacggtcta aaagttttgt attggtatgt tctggtgcat
    14041 aaatattttt gtattcaagt cccttttttt tagtgtagtc ctgagtcttt ccaaaagtca
    14101 gatcctctaa tttttcacag gcacgataag cagcacaagc aacggctgaa cgtccgtcac
    14161 ttcgtgagat gttgctttca ttcaaatgaa aaattgccac tttaaaaacc taaatttctg
    14221 cataccgtgg ttttgatttt tgcaactttg aaaaagttgc tgaccatgct aaagcgaggt
    14281 catggggtac aggggaaacc cctgtcgcaa cgtccacttt tgaatgaaat gataaaagtg
    14341 ggtaagtgcg cccttcgttt cactccggga aaacttcgct tcgctacgtt tgatattgct
    14401 cgctttgttt tttgactcta tgatgatgtt tgattattga tcatcacggt ttaatactat
    14461 gtcagatatt caagaaaaga aagatgcagt tttaaagaaa ctcaccaaag cacagcagaa
    14521 aaaattatcc ctgcaagagc aaattgagta tttaaaaaaa gaacaagagc agatcttaga
    14581 acaggcaaaa cagaaacaga atgagcttaa actcaaacag caaaaacttt taagcgaagc
    14641 aaaagcccaa gaacggaaaa atgaaactag acggaaaatt ttgcttggcg cttgggtttt
    14701 gaataaattt aagaatgatg aatcttttaa acatcaatgg gctgattttg agcagttttt
    14761 aagtgcggaa agtaagacag aagaaaatag acagaaagat aaagagcttt ttaataatct
    14821 ttttaaggga aagttctaaa tttcatataa gagattttat gttaaatcga taatcaaaaa
    14881 gcccatgaaa gtaagccttt tttcatccat gagatttact tacttcttct aaaattttgc
    14941 tttttaccca gtttgaaaaa tctttttgat tttctacaaa gtgtaaaaga tcagcctcac
    15001 tttcccgatt aaaacttata cttttaactt ttctactctg cttttcatat tttgcattta
    15061 ttgcaatttt tcgccaattt gtttgatcga caatatcata agcagcagcg aaaacattat
    15121 ttgcatcatt ttcatcaata caaacttcaa tattttctgc attgaaaata cggatttcat
    15181 ccatttctat ttttttattg gtagatgaag tgaacccgaa aaataaggta ataccatgtt
    15241 ttgatgttat ttctatattg ccaaaagatt catcagtatt tgtatgagat tgatctaata
    15301 gaatttcaca gctagtaact cgccaacctg tttcctgttg aagttgtgaa gctaacattg
    15361 gaagcatatg taatccattt ttgtttttga tagtaatacg ataatattaa tttttaattt
    15421 ttacaagttt atagatgagg atatagacat tttaattggt gagaatatag acactttaaa
    15481 tgggttctaa catgaaaaat aggaacttaa aattcctgtt tttattggtt tttaagtttt
    15541 aagcatattg catgataagc tttaaatcag aagtattcta agttctaaag ccagttagct
    15601 actccaccat ggccagaaca agtacctctc ctattcaagc taaaagacca agtcccatct
    15661 ctacacttcg ctgttgcatt agaaggttgc ttatttgctg aaccgtaccg ggtttgtcgg
    15721 agagtcaata ttctgagaga ctatcccgat gacaaaatta aaatatgccc ctgaaatcag
    15781 agaaagagcg gttcaattat tgattgaatc tgaaaaagat tatccatcga attgggctgc
    15841 gatcaccgct attgctccca agataggttg tactcctgaa acactacgtg tttggtatca
    15901 aaaatattta gataaactaa atccagttaa agtacagcag ctttcagacc aagaacgtat
    15961 caaacaactc gaacgcgaaa ataaagaact gcaacgcgcc aatgagattc tacgtaaagc
    16021 agccgctttt ttcgcccagg cggaactcga ccgcccacac aaataatggt ggattttatc
    16081 cataataata aagagctgta cggagtcgag gcgatttgta gaattttacc gatcgcaccc
    16141 tcaacctatt accggactct agatctctgc gaaaacccag aacatcgagc aaagcgagat
    16201 ctacatgact tgcatcatgc tgaacaaatt aaacgaattt ggaaggaaag ttcaggtcga
    16261 tatggtgtac gtaaagtttg gcaaaaactg aaacgtgaag gctatattat tgcacgctgt
    16321 acagttgctc gattgatgca aaagctaggt atacaaggtg tttggcgagg taagaataaa
    16381 caaactaccc atagccgaga tgatcaaaaa cgagcagatg acttggtgaa acgcaatttt
    16441 agtgctgatc accctgacca gctgtgggtc gctgacttta cgtatattca aacaaattca
    16501 ggctgggtct ataccgcctt tattattgat gtgttctcgc gagcaattgt tggatggaaa
    16561 gtatcaacac ggatgaatac agacatggtg ctcgatgcac tggagcaagc attgcatgat
    16621 cgaggcatgc caaagaacgt gattcatcat agtgacagag gtgtgcaata tctttccatt
    16681 cgttatacca atcgtttaga agcagcaaat ttacgagcat cagtcggtac gaccggtgat
    16741 tcatacgata atgctttggc tgaaacagtg aatggcttat acaaaacaga ggtgattgaa
    16801 tatttaaaag cagattggca aggtttagcg gatgtacaac ttgcgacatt aaattgggta
    16861 gattggttca ataaagagcg tgtacatagt gcactaggtt atgtgccacc ttttgatttt
    16921 gaagcaatgt actatgataa aattaatccg ttaggtcagg tggcctaact taaataaaaa
    16981 agtctccgac aaacccggta cggttcagat caccgcaaac aggttactcg cctggggatt
    17041 ccctttcgac ccgagcatcc gtatgagact catgctcgat tattattatt atagaagccc
    17101 ccatgaataa atcgctcatc attttcggca tcgtcaacat aacctcggac agtttctccg
    17161 atggaggccg gtatctggcg ccagacgcag ccattgcgca ggcgcgtaag ctgatggccg
    17221 agggggcaga tgtgatcgac ctcggtccgg catccagcaa tcccgacgcc gcgcctgttt
    17281 cgtccgacac agaaatcgcg cgtatcgcgc cggtgctgga cgcgctcaag gcagatggca
    17341 ttcccgtctc gctcgacagt tatcaacccg cgacgcaagc ctatgccttg tcgcgtggtg
    17401 tggcctatct caatgatatt cgcggttttc cagacgctgc gttctatccg caattggcga
    17461 aatcatctgc caaactcgtc gttatgcatt cggtgcaaga cgggcaggca gatcggcgcg
    17521 aggcacccgc tggcgacatc atggatcaca ttgcggcgtt ctttgacgcg cgcatcgcgg
    17581 cgctgacggg tgccggtatc aaacgcaacc gccttgtcct tgatcccggc atggggtttt
    17641 ttctgggggc tgctcccgaa acctcgctct cggtgctggc gcggttcgat gaattgcggc
    17701 tgcgcttcga tttgccggtg cttctgtctg tttcgcgcaa atcctttctg cgcgcgctca
    17761 caggccgtgg tccgggggat gtcggggccg cgacactcgc tgcagagctt gccgccgccg
    17821 caggtggagc tgacttcatc cgcacacacg agccgcgccc cttgcgcgac gggctggcgg
    17881 tattggcggc gctgaaagaa accgcaagaa ttcgttaact gcacattcgg gatatttctc
    17941 tatattcgcg gttcagcagg catgtcccct ttgagggcga cccgacgaca ggataatcga
    18001 ccttatggtg cgcaaatatt tcggcacaga cggtattcgt ggcaaagcca acgaaggcgc
    18061 gatgacggcg gaaaccgcct tgcgcgtcgg catggcggct ggccgtgtct ttcgtcgcgg
    18121 tgaccaccgc catcgtgtcg tgatcggcaa ggatacgcgc ctgtcgggct atatgcttga
    18181 acccgcgctc acagccggtt tcacctcgat ggggctttgt tgcacaaacc tatctgtaaa
    18241 ggctttttca gcgatataat tttcaaatga agaagcctac acacaaaatc taccgcacaa
    18301 ccaattggcc cgcatataac cgagcactca tgagtcgcgg aaatattgcc atttggtttg
    18361 atcctgctac gcaatggtat gctccatcaa aaggcaaaca agggcgaaat caaacctact
    18421 ccgacgcagc tatccaatgc tgcttaatga ttaaatctct attccgtttg tctttacgta
    18481 tggtcactgg ctttgtgcaa agtctgatta aactttgcgg attaaattgg accgcaccag
    18541 attacagtac gctttgtaga agacaaaagc atattgatat tgcaatcagc taccaaaaaa
    18601 gtagcgatgg gctgcatcta ctcgtagact ctacaggcat gaagtttcta ggtgagggcg
    18661 aatggaaacg caagaaacat ggagctgaat atcgtcgcca atggcgtaaa ctacatattg
    18721 gtatagatgc caaaacccta caaatacgcg ctattcagct c
//
