LOCUS       NZ_CP035657             6303 bp    DNA     circular CON 17-JUN-2024
DEFINITION  Enterococcus faecium strain UAMSEF_09 plasmid unnamed3, complete
            sequence.
ACCESSION   NZ_CP035657
VERSION     NZ_CP035657.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN10863492
            Assembly: GCF_005886715.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6303)
  AUTHORS   Udaondo,Z., Abram,K., Kothari,A. and Jun,S.R.
  TITLE     Top-Down Genomic Surveillance Approach To Investigate the Genomic
            Epidemiology and Antibiotic Resistance Patterns of Enterococcus
            faecium Detected in Cancer Patients in Arkansas
  JOURNAL   Microbiol Spectr 11 (3), e0490122 (2023)
   PUBMED   36995227
REFERENCE   2  (bases 1 to 6303)
  AUTHORS   Jenjaroenpun,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2019) Biomedical Informatics, University of
            Arkansas for Medical Sciences, 4301 W Markham St, Little Rock, AR
            72205, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: JAN-2019
            Assembly Method                   :: Unicycler v. 0.4.4; Canu v. 1.8
            Assembly Name                     :: UAMS_Efae_0.4.4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 747.0x
            Sequencing Technology :: Oxford Nanopore MiniION; Illumina NextSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_005886715.1-RS_2024_06_17
            Annotation Date                   :: 06/17/2024 10:49:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,330
            CDSs (total)                      :: 3,237
            Genes (coding)                    :: 2,974
            CDSs (with protein)               :: 2,974
            Genes (RNA)                       :: 93
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 155 of 263
            Pseudo Genes (incomplete)         :: 97 of 263
            Pseudo Genes (internal stop)      :: 56 of 263
            Pseudo Genes (multiple problems)  :: 38 of 263
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP035657.1.
            Bacteria and source DNA available from Tip's lab.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="UAMSEF_09"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="unnamed3"
                     /geo_loc_name="USA:Arkansas"
                     /collection_date="2018-09-18"
     gene            complement(join(5752..6303,1..399))
                     /locus_tag="EWM55_RS00495"
                     /old_locus_tag="EWM55_00495"
     CDS             complement(join(5752..6303,1..399))
                     /locus_tag="EWM55_RS00495"
                     /old_locus_tag="EWM55_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_033642128.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVRL
                     TFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIRGT
                     AKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFAYFS
                     KEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVVGYQF
                     TFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELELLQQI
                     KQLEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ"
                     /name="replication initiation protein"
     gene            1075..1161
                     /locus_tag="EWM55_RS00500"
                     /old_locus_tag="EWM55_00500"
     CDS             1075..1161
                     /locus_tag="EWM55_RS00500"
                     /old_locus_tag="EWM55_00500"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_107532029.1"
                     /translation="MFERFICPLLVEVCVSLFSYWLNNRNKK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(1282..1971)
                     /locus_tag="EWM55_RS00505"
                     /old_locus_tag="EWM55_00505"
     CDS             complement(1282..1971)
                     /locus_tag="EWM55_RS00505"
                     /old_locus_tag="EWM55_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002321377.1"
                     /translation="MAFYLPYLLISASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLVG
                     VIFALSIVLSGLFGVLEGNKRVSRSILLGDITQKYEAACDKKKKEQALAQKMEEFTACY
                     EDMNNIFVDQEKRLTDKNMKKLTRFYQNLPEKQQKEVQEKYEQVKKDVQYFKDTQTEEA
                     CCNLFSDTIPFSTSEQERKERQQSVTYERYETLLQQATNIQNPTKKETALNYLESVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     gene            complement(1978..2256)
                     /locus_tag="EWM55_RS00510"
                     /old_locus_tag="EWM55_00510"
     CDS             complement(1978..2256)
                     /locus_tag="EWM55_RS00510"
                     /old_locus_tag="EWM55_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_058138385.1"
                     /translation="MNEEQTTNKNYRSKTLKDIENEIKRQRAFLAPYTQERIQTTMDSQ
                     VKKLHQALLDQRNQEQKKALIQRSMDILAPLCCLVLTLLAFWLVWGE"
                     /name="hypothetical protein"
     gene            complement(2216..3130)
                     /locus_tag="EWM55_RS00515"
                     /old_locus_tag="EWM55_00515"
     CDS             complement(2216..3130)
                     /locus_tag="EWM55_RS00515"
                     /old_locus_tag="EWM55_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_002321380.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIRT
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPNHQIAIYTHTDKDHIHNHI
                     VINSVNLETGKKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKTEKRTMSEIKLKEK
                     NEPVWKDEIRFAIDQTMKNKKTRTYTQFCDSLKTFGIHCLNRGKNFTYELVHKKKKVRS
                     NKLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTFTPEQSEI
                     RPTRRNHEQHVERGTDHQQELSL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3112..3492)
                     /locus_tag="EWM55_RS00520"
                     /old_locus_tag="EWM55_00520"
     CDS             complement(3112..3492)
                     /locus_tag="EWM55_RS00520"
                     /old_locus_tag="EWM55_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_002321381.1"
                     /translation="MSEQHQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPDDA
                     QRLSENLKIMQKELQKIWQQLK"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(3814..4017)
                     /locus_tag="EWM55_RS00525"
                     /old_locus_tag="EWM55_00525"
     CDS             complement(3814..4017)
                     /locus_tag="EWM55_RS00525"
                     /old_locus_tag="EWM55_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002321382.1"
                     /translation="MFLISKSLKSLKTFTKKNPQIATIRIFIFAIAVAIIVNMLSGGQL
                     YKSGLFLVLILVVLDIIKSKKK"
                     /name="hypothetical protein"
     gene            complement(4029..4562)
                     /locus_tag="EWM55_RS00530"
                     /old_locus_tag="EWM55_00530"
     CDS             complement(4029..4562)
                     /locus_tag="EWM55_RS00530"
                     /old_locus_tag="EWM55_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002334266.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENVV
                     IEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAINAA
                     FWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQRGY
                     DAAGRVVATRYSTL"
                     /name="hypothetical protein"
     gene            complement(5012..5182)
                     /locus_tag="EWM55_RS17415"
     CDS             complement(5012..5182)
                     /locus_tag="EWM55_RS17415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002292472.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNSP
                     NEELFVKETDK"
                     /name="hypothetical protein"
     gene            complement(5198..5731)
                     /locus_tag="EWM55_RS00535"
                     /old_locus_tag="EWM55_00535"
     CDS             complement(5198..5731)
                     /locus_tag="EWM55_RS00535"
                     /old_locus_tag="EWM55_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_002321384.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKVV
                     LRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLREQL
                     ALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATETID
                     KQIDTEKKWWQLWK"
                     /name="DUF536 domain-containing protein"
     Typing          complement(2220..3130)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.079"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gagtgaatag cgaacccact catctaaatt gtttagtaaa ggtaaagctt tttcgtaaac
       61 ttgtatgtct acatatggat ctttagcagt tccacgtatt ttaaactcac taaacataac
      121 gaatctatct atatcgagcc cattagcact tcttttacca aaacgtaaat ccattaattt
      181 atcataagtc atttctaaat cattaatgaa acgattatta gcagttgctt tataattact
      241 taaatctttt agttggtcaa aagtaagacg gactgtttta tcgccttttt cgcgcattct
      301 agacacaata gagaaaaata gattcatttc aactggtgta aatttccgca acggaatggt
      361 atttaattca tgatggtatt tcacgatttc atttgccaaa gatattcctc cttactcctt
      421 caatggatac agacgttata aacaacgttt tgtctgtgat aatgcatatt ataacatacg
      481 tgagtgcaaa aaaagagttt aaaagcagac aaaatgtagt ttaaaagcag acaaaatgta
      541 gtttaaaagc agacaaaatg tagtttaaaa gcagacaaaa tgtagtttat aaaacgctgt
      601 atccattgaa ggagtaagga cacagccgac ggtaaagagg tttaaaaaaa ggtttaaaaa
      661 gagattataa aagagggtga caaaatttcc cgcctaaaaa ccacttcttt tttctaccaa
      721 caggttcgcg catttttcaa aactgccaat tcaaaacctt gctccctacg gtcgctaaaa
      781 aagtgtgatc gctatgctct cacattgtac tgcgcagtcg cctaaaggct cctttgctaa
      841 aaacaagtca gtaacggagg tgggggaagc ctctgatagc tcgtctaagt cgttttaaga
      901 cagtcttggg taaaaaacct tatcttgttc ttaaagcgcc ttacaggcgg tcagggcaag
      961 ccctaacaac ccgaaaataa gggatactta aaaacaccac acagatttta ttttgtaagg
     1021 tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta tctaatgttt
     1081 gaacggttca tttgccccct tcttgtagaa gtttgtgtct ctttattcag ttattggttg
     1141 aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa aaagccccag
     1201 agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc aggttcagtt
     1261 taacatgatt gctaatcaaa ttcaattttg ttgttgctga tccaaccatt ccttgacact
     1321 ttctagataa tttaatgccg tttctttttt cgttggattt tgtatgtttg ttgcttgctg
     1381 aagcagtgtt tcataacgtt cgtatgtgac cgattgttgc cgttcttttc gttcttgttc
     1441 actggttgaa aaagggattg tatcgctaaa taagttacaa caagcttctt ctgtctgcgt
     1501 atctttaaaa tattgcacgt ctttttttac ttgctcatat ttttcttgta cctctttttg
     1561 ctgtttttct ggtaagtttt gataaaaacg agtgagtttt ttcatgtttt tatctgttaa
     1621 acgtttctct tgatccacaa aaatattgtt catgtcctca taacaagcgg taaattcctc
     1681 cattttttgt gctaatgctt gctctttttt ctttttatca catgctgctt catacttttg
     1741 agtgatatcc cctaataaaa tgcttcttga aacacgttta ttaccctcta aaacgccgaa
     1801 taaaccactt aaaacgattg ataaggcaaa tataaccccg accaataaaa atcgcttaaa
     1861 tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc taagccaaat
     1921 aattcctaaa atactggcag aaatgagtag atagggtaaa tagaatgcca tgtctgttta
     1981 ttctccccat accaaccaaa aggctaataa tgttagtact aagcaacata agggggcaag
     2041 aatatccatc gatcgttgaa ttaaagcctt tttttgttct tggtttcgct ggtctaatag
     2101 cgcttggtgg agttttttga cttgtgaatc catcgtagtt tgtatccttt cttgggtata
     2161 aggcgcaaga aaggcacgtt gtctctttat ttcgttttcg atgtctttta acgttttaga
     2221 gcgataattc ttgttggtgg tctgttcctc gttcaacgtg ttgttcatgg tttcttcgag
     2281 tcggtctaat ttccgattgt tcaggcgtaa aagttcgttg ttctcttcgt acttcttctg
     2341 cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt tgattgtagg
     2401 ttcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc tttcctagtt
     2461 tgttggatcg tacttttttc tttttgtgga ccaattcgta agtaaaattt tttccgcgat
     2521 tcaagcaatg aatgccgaat gttttaagtg aatcacaaaa ttgggtatac gttcgagttt
     2581 ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa accggttcat
     2641 ttttttcttt taatttaatt tcagacatgg ttcgtttttc ggttttcttt cctcgttccg
     2701 tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat ttgtttaaaa
     2761 atgcttgccc atgggcttga aattttttcc cagtttcaag attaacggag ttaatgacaa
     2821 tgtgattatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg tggttagggg
     2881 ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta atttctcctg
     2941 gtcgaaagga ctgaatcaat aagtgtgctt gtacacgatc gtcttttcca taaagcgtgc
     3001 gtatttgttt catttcactt ttggcataat caatgtcaca gtttattcct gttttgacgg
     3061 ttgctcgtgg ttctgcgtaa ttgattgctc ggctacatga agtggttcga cttactttaa
     3121 ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg ataaacgttg
     3181 agcatcatca ggagcaacgt cgtgttggtg tgtattgcac cacttggcaa tttgattgat
     3241 attccctcca gctttggcta aatcgcgcgc gattgcctgg gcaatgtctg gcgccacttt
     3301 cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca cactcatttg
     3361 caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc gaaaactgat
     3421 ttgttttggc tcggatcgat aggtatattt tttagaatga tcgctagcca aattttgatg
     3481 ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt tcgcaatacc
     3541 attgtaccta aaaaggtaca atgagtaaag ctcactaaaa gggtttggca agctctatat
     3601 ttgctagcca aatacctccg tatttggtgc aaataaccgc ttgtcaaagg agcgcaagct
     3661 cccccttgaa tccccctcaa aatccacgct ccacaataca aaaaaaggct agcatttgct
     3721 agccactttt tgtaaagttt cgcttatttc accgaaggtg aaattgttgc tttgtaatat
     3781 tcaaacaacc ctattcttct aaaatgtatc gatttatttt tttttagact ttattatgtc
     3841 taagacaact aagattaaca ctaaaaataa accagatttg tataattgcc ctccgcttag
     3901 catattaaca attatagcaa cagctatagc aaatataaaa atacgaatag tagctatttg
     3961 cggattcttt ttagtaaatg tttttaatga ttttaatgat ttagatatca aaaacatcac
     4021 tccttaactt ataacgttga atatctagta gctacaactc ttccagctgc atcatatccc
     4081 ctttggtaat aaaatccaat ccagccatta ccatttttgt cccattcttt tgcaactgca
     4141 ttccctggag aagcaccttt tttagtccaa cttgcacctg ttaacaaagc accaattgcc
     4201 caacctggga taccaaacat agaagtgcta gctttccctc ctgcccaaaa ggcagcatta
     4261 atagcattag cagctactcc agtggttaca gcaatatttg tatattcctt tttgccccat
     4321 cttgcaactg cagctcttgt catgatatct gaagaatttg caaaatcttc cgcactataa
     4381 ccatcctcat atttagttac gaccatacca tcttcagtgt actcaataac tacattttca
     4441 gtagcattgt tttcgtttaa tgataccact tgggaatttt ctgaagcata aactatagta
     4501 gttgacggta aaacaactgc cccaatagtt aacaaagcta cccctaaagt aaattttttc
     4561 atagaatttc ctcctgatac agaaaaatat atagaaaacg ttttctttgt gaaaatcata
     4621 aaacaaaagg tataggcttg tcaatatttt atttagtaaa gataatttat atttttgata
     4681 atgtggtata gttagaatgc attaggaaaa tatgttatat taaattcttg ttgacataat
     4741 gtacactatg ccaagtagcc cttgtatcaa ccgatacaga ggctcacttt gtgattttcg
     4801 attggtctag gtcaaaagct ctttttaagc ttgattttct gcggttgctt ctttccgttt
     4861 ctttgtgaat ttttaccagt cattttttac tgggttgtta tttttaataa aatatgataa
     4921 atttttggta gcgcttcata agcgcgttgc tttttcaaga ataaacactc attatttttt
     4981 aactggctgc tcgaaagagc agtctttttt attatttatc tgtttctttt acaaacaatt
     5041 cttcgttagg actgttgtaa taatttggat tcaatttcaa ttcaccatgt ttattccctt
     5101 tattgatcac tgacaataac atcggactga atcctagaat tcctagtaaa atagaaccga
     5161 atagaagatt ttttcttgtc atgaatgttc cctccagtta tttccaaagt tgccaccatt
     5221 ttttttcagt atcaatttgt ttgtctattg tttctgttgc ttcagaaaga gtcgttgagt
     5281 cattctctgg agcttcttct gtataagtca actgtagttt ttcttgcagt ttttcgattt
     5341 gtttgttggc ttgtagggtg agttgttgtt gctggtcgag tagtttttgt atatgagaat
     5401 tattttgctc tctgagggca agttgttctc gtaaaaattc attttcttca cgcaactgat
     5461 ctagcaactt gtttgtaacc acgtttttgc ttggtatatt aacattttta tcgttgtctt
     5521 gttcgtactc gtgcaaccta tctagcaaca tttggttgcc taagttgcga gcaagttgca
     5581 atactccaga acgcaataca accttataac cacgctcatt tttagctatc atggaagaat
     5641 ctaatttttg gatatgtttc gatattgctt gcctactcat cttaaaaagt tctgacaatt
     5701 cctttattgt aaaggtttct tgtggttgca tactggttgc ctcctaggtt gctactggaa
     5761 taaattatct aaatcagata ataaactttt tttctctttt ttcgaaagtg cggactctgt
     5821 ttgtttttca agagcttctt tctctttttc tagttgtttg atttgttgga ggagttcaag
     5881 ttctcgtttc ttctgtttga ttttttcgtt aattgttgta gcaggttctt cgtattcttt
     5941 ttgtttatca gctaaagcca atgctttatt ttgttcttct ttggttggtg catgtttatt
     6001 aaaatcatct gcttttttga tttctggttt aaacgtaaat tgatagccaa cgacaggttt
     6061 tcctcgccct tttccgtatt tctttttgat tgataatcct ttgaagattg cggttaattc
     6121 ctgtcgtata ggctttataa cacgattatc aacgtttgct tggttgttcc agtaactttt
     6181 tggaatatct aatagttcat gaaaatcctc tttagaaaaa taagcaaaac ctttagttcg
     6241 atattgtttt agtaatcgaa acatagtctt tgcatagcta ctttgtaatt cgttaaattg
     6301 ttg
//
