LOCUS       NZ_CP035651             6303 bp    DNA     circular CON 17-JUN-2024
DEFINITION  Enterococcus faecium strain UAMSEF_08 plasmid unnamed3, complete
            sequence.
ACCESSION   NZ_CP035651
VERSION     NZ_CP035651.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN10863491
            Assembly: GCF_005886655.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6303)
  AUTHORS   Udaondo,Z., Abram,K., Kothari,A. and Jun,S.R.
  TITLE     Top-Down Genomic Surveillance Approach To Investigate the Genomic
            Epidemiology and Antibiotic Resistance Patterns of Enterococcus
            faecium Detected in Cancer Patients in Arkansas
  JOURNAL   Microbiol Spectr 11 (3), e0490122 (2023)
   PUBMED   36995227
REFERENCE   2  (bases 1 to 6303)
  AUTHORS   Jenjaroenpun,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2019) Biomedical Informatics, University of
            Arkansas for Medical Sciences, 4301 W Markham St, Little Rock, AR
            72205, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: JAN-2019
            Assembly Method                   :: Unicycler v. 0.4.4
            Assembly Name                     :: UAMS_Efae_0.4.4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 483.0x
            Sequencing Technology :: Oxford Nanopore MiniION; Illumina NextSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_005886655.1-RS_2024_06_17
            Annotation Date                   :: 06/17/2024 10:52:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,114
            CDSs (total)                      :: 3,023
            Genes (coding)                    :: 2,796
            CDSs (with protein)               :: 2,796
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 227
            CDSs (without protein)            :: 227
            Pseudo Genes (ambiguous residues) :: 0 of 227
            Pseudo Genes (frameshifted)       :: 133 of 227
            Pseudo Genes (incomplete)         :: 91 of 227
            Pseudo Genes (internal stop)      :: 43 of 227
            Pseudo Genes (multiple problems)  :: 33 of 227
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP035651.1.
            Bacteria and source DNA available from Tip's lab.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="UAMSEF_08"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="unnamed3"
                     /geo_loc_name="USA:Arkansas"
                     /collection_date="2018-07-07"
     gene            join(5540..6303,1..187)
                     /locus_tag="EWM54_RS00225"
                     /old_locus_tag="EWM54_00225"
     CDS             join(5540..6303,1..187)
                     /locus_tag="EWM54_RS00225"
                     /old_locus_tag="EWM54_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_033642128.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVRL
                     TFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIRGT
                     AKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFAYFS
                     KEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVVGYQF
                     TFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELELLQQI
                     KQLEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ"
                     /name="replication initiation protein"
     gene            208..741
                     /locus_tag="EWM54_RS00230"
                     /old_locus_tag="EWM54_00230"
     CDS             208..741
                     /locus_tag="EWM54_RS00230"
                     /old_locus_tag="EWM54_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_002321384.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKVV
                     LRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLREQL
                     ALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATETID
                     KQIDTEKKWWQLWK"
                     /name="DUF536 domain-containing protein"
     gene            757..927
                     /locus_tag="EWM54_RS16325"
     CDS             757..927
                     /locus_tag="EWM54_RS16325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002292472.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNSP
                     NEELFVKETDK"
                     /name="hypothetical protein"
     gene            1377..1910
                     /locus_tag="EWM54_RS00235"
                     /old_locus_tag="EWM54_00235"
     CDS             1377..1910
                     /locus_tag="EWM54_RS00235"
                     /old_locus_tag="EWM54_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002334266.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENVV
                     IEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAINAA
                     FWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQRGY
                     DAAGRVVATRYSTL"
                     /name="hypothetical protein"
     gene            1922..2125
                     /locus_tag="EWM54_RS00240"
                     /old_locus_tag="EWM54_00240"
     CDS             1922..2125
                     /locus_tag="EWM54_RS00240"
                     /old_locus_tag="EWM54_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002321382.1"
                     /translation="MFLISKSLKSLKTFTKKNPQIATIRIFIFAIAVAIIVNMLSGGQL
                     YKSGLFLVLILVVLDIIKSKKK"
                     /name="hypothetical protein"
     gene            2447..2827
                     /locus_tag="EWM54_RS00245"
                     /old_locus_tag="EWM54_00245"
     CDS             2447..2827
                     /locus_tag="EWM54_RS00245"
                     /old_locus_tag="EWM54_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_002321381.1"
                     /translation="MSEQHQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPDDA
                     QRLSENLKIMQKELQKIWQQLK"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2809..3723
                     /locus_tag="EWM54_RS00250"
                     /old_locus_tag="EWM54_00250"
     CDS             2809..3723
                     /locus_tag="EWM54_RS00250"
                     /old_locus_tag="EWM54_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_002321380.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIRT
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPNHQIAIYTHTDKDHIHNHI
                     VINSVNLETGKKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKTEKRTMSEIKLKEK
                     NEPVWKDEIRFAIDQTMKNKKTRTYTQFCDSLKTFGIHCLNRGKNFTYELVHKKKKVRS
                     NKLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTFTPEQSEI
                     RPTRRNHEQHVERGTDHQQELSL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3968..4657
                     /locus_tag="EWM54_RS00260"
                     /old_locus_tag="EWM54_00260"
     CDS             3968..4657
                     /locus_tag="EWM54_RS00260"
                     /old_locus_tag="EWM54_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002321377.1"
                     /translation="MAFYLPYLLISASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLVG
                     VIFALSIVLSGLFGVLEGNKRVSRSILLGDITQKYEAACDKKKKEQALAQKMEEFTACY
                     EDMNNIFVDQEKRLTDKNMKKLTRFYQNLPEKQQKEVQEKYEQVKKDVQYFKDTQTEEA
                     CCNLFSDTIPFSTSEQERKERQQSVTYERYETLLQQATNIQNPTKKETALNYLESVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     gene            complement(4778..4864)
                     /locus_tag="EWM54_RS16800"
                     /old_locus_tag="EWM54_00265"
     CDS             complement(4778..4864)
                     /locus_tag="EWM54_RS16800"
                     /old_locus_tag="EWM54_00265"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_107532029.1"
                     /translation="MFERFICPLLVEVCVSLFSYWLNNRNKK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     Typing          5542..6303
                     /name="rep_cluster_1197"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="81.688"
                     /coverage="99.0"
                     /evalue="1.41e-175"
     Typing          2810..3720
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.079"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 agaatacgaa gaacctgcta caacaattaa cgaaaaaatc aaacagaaga aacgagaact
       61 tgaactcctc caacaaatca aacaactaga aaaagagaaa gaagctcttg aaaaacaaac
      121 agagtccgca ctttcgaaaa aagagaaaaa aagtttatta tctgatttag ataatttatt
      181 ccagtagcaa cctaggaggc aaccagtatg caaccacaag aaacctttac aataaaggaa
      241 ttgtcagaac tttttaagat gagtaggcaa gcaatatcga aacatatcca aaaattagat
      301 tcttccatga tagctaaaaa tgagcgtggt tataaggttg tattgcgttc tggagtattg
      361 caacttgctc gcaacttagg caaccaaatg ttgctagata ggttgcacga gtacgaacaa
      421 gacaacgata aaaatgttaa tataccaagc aaaaacgtgg ttacaaacaa gttgctagat
      481 cagttgcgtg aagaaaatga atttttacga gaacaacttg ccctcagaga gcaaaataat
      541 tctcatatac aaaaactact cgaccagcaa caacaactca ccctacaagc caacaaacaa
      601 atcgaaaaac tgcaagaaaa actacagttg acttatacag aagaagctcc agagaatgac
      661 tcaacgactc tttctgaagc aacagaaaca atagacaaac aaattgatac tgaaaaaaaa
      721 tggtggcaac tttggaaata actggaggga acattcatga caagaaaaaa tcttctattc
      781 ggttctattt tactaggaat tctaggattc agtccgatgt tattgtcagt gatcaataaa
      841 gggaataaac atggtgaatt gaaattgaat ccaaattatt acaacagtcc taacgaagaa
      901 ttgtttgtaa aagaaacaga taaataataa aaaagactgc tctttcgagc agccagttaa
      961 aaaataatga gtgtttattc ttgaaaaagc aacgcgctta tgaagcgcta ccaaaaattt
     1021 atcatatttt attaaaaata acaacccagt aaaaaatgac tggtaaaaat tcacaaagaa
     1081 acggaaagaa gcaaccgcag aaaatcaagc ttaaaaagag cttttgacct agaccaatcg
     1141 aaaatcacaa agtgagcctc tgtatcggtt gatacaaggg ctacttggca tagtgtacat
     1201 tatgtcaaca agaatttaat ataacatatt ttcctaatgc attctaacta taccacatta
     1261 tcaaaaatat aaattatctt tactaaataa aatattgaca agcctatacc ttttgtttta
     1321 tgattttcac aaagaaaacg ttttctatat atttttctgt atcaggagga aattctatga
     1381 aaaaatttac tttaggggta gctttgttaa ctattggggc agttgtttta ccgtcaacta
     1441 ctatagttta tgcttcagaa aattcccaag tggtatcatt aaacgaaaac aatgctactg
     1501 aaaatgtagt tattgagtac actgaagatg gtatggtcgt aactaaatat gaggatggtt
     1561 atagtgcgga agattttgca aattcttcag atatcatgac aagagctgca gttgcaagat
     1621 ggggcaaaaa ggaatataca aatattgctg taaccactgg agtagctgct aatgctatta
     1681 atgctgcctt ttgggcagga gggaaagcta gcacttctat gtttggtatc ccaggttggg
     1741 caattggtgc tttgttaaca ggtgcaagtt ggactaaaaa aggtgcttct ccagggaatg
     1801 cagttgcaaa agaatgggac aaaaatggta atggctggat tggattttat taccaaaggg
     1861 gatatgatgc agctggaaga gttgtagcta ctagatattc aacgttataa gttaaggagt
     1921 gatgtttttg atatctaaat cattaaaatc attaaaaaca tttactaaaa agaatccgca
     1981 aatagctact attcgtattt ttatatttgc tatagctgtt gctataattg ttaatatgct
     2041 aagcggaggg caattataca aatctggttt atttttagtg ttaatcttag ttgtcttaga
     2101 cataataaag tctaaaaaaa aataaatcga tacattttag aagaataggg ttgtttgaat
     2161 attacaaagc aacaatttca ccttcggtga aataagcgaa actttacaaa aagtggctag
     2221 caaatgctag cctttttttg tattgtggag cgtggatttt gagggggatt caagggggag
     2281 cttgcgctcc tttgacaagc ggttatttgc accaaatacg gaggtatttg gctagcaaat
     2341 atagagcttg ccaaaccctt ttagtgagct ttactcattg taccttttta ggtacaatgg
     2401 tattgcgaac gatacgtccg taggacgtac tgaaagggtg ggacttatga gcgaacaaca
     2461 tcaaaatttg gctagcgatc attctaaaaa atatacctat cgatccgagc caaaacaaat
     2521 cagttttcga gtgagcgaat ccgaatttgc aaagctgaag cagtcagctg aagctttgca
     2581 aatgagtgtg ccggctttcg tgaaagccaa ggcacaaaac gcgcgtctgg tgacaccgaa
     2641 agtggcgcca gacattgccc aggcaatcgc gcgcgattta gccaaagctg gagggaatat
     2701 caatcaaatt gccaagtggt gcaatacaca ccaacacgac gttgctcctg atgatgctca
     2761 acgtttatcc gaaaacctaa aaatcatgca aaaggagcta caaaagatat ggcaacagtt
     2821 aaagtaagtc gaaccacttc atgtagccga gcaatcaatt acgcagaacc acgagcaacc
     2881 gtcaaaacag gaataaactg tgacattgat tatgccaaaa gtgaaatgaa acaaatacgc
     2941 acgctttatg gaaaagacga tcgtgtacaa gcacacttat tgattcagtc ctttcgacca
     3001 ggagaaatta ccgctgaaaa agccaatcaa ctaggggcag agtatgcgaa aaaaatagcc
     3061 cctaaccacc aaatcgcgat ttacacacat acagacaaag accatattca taatcacatt
     3121 gtcattaact ccgttaatct tgaaactggg aaaaaatttc aagcccatgg gcaagcattt
     3181 ttaaacaaat gttatgacct aaatgacgag atttgtcttg aacatgactt aacaatcacg
     3241 gaacgaggaa agaaaaccga aaaacgaacc atgtctgaaa ttaaattaaa agaaaaaaat
     3301 gaaccggttt ggaaagacga aattcggttt gcgattgatc aaacaatgaa aaacaaaaaa
     3361 actcgaacgt atacccaatt ttgtgattca cttaaaacat tcggcattca ttgcttgaat
     3421 cgcggaaaaa attttactta cgaattggtc cacaaaaaga aaaaagtacg atccaacaaa
     3481 ctaggaaagg attacgaaaa ggagacgatt ttacatgagc tggacagacg agaacgaacc
     3541 tacaatcaac aacgaaccaa cgagctacga agagctcgag ctagcattcg cgaaactgca
     3601 gaagaagtac gaagagaaca acgaactttt acgcctgaac aatcggaaat tagaccgact
     3661 cgaagaaacc atgaacaaca cgttgaacga ggaacagacc accaacaaga attatcgctc
     3721 taaaacgtta aaagacatcg aaaacgaaat aaagagacaa cgtgcctttc ttgcgcctta
     3781 tacccaagaa aggatacaaa ctacgatgga ttcacaagtc aaaaaactcc accaagcgct
     3841 attagaccag cgaaaccaag aacaaaaaaa ggctttaatt caacgatcga tggatattct
     3901 tgccccctta tgttgcttag tactaacatt attagccttt tggttggtat ggggagaata
     3961 aacagacatg gcattctatt taccctatct actcatttct gccagtattt taggaattat
     4021 ttggcttagc tataaggctt atcaaacaag atattcatta aaaggctcta aaattcgatt
     4081 taagcgattt ttattggtcg gggttatatt tgccttatca atcgttttaa gtggtttatt
     4141 cggcgtttta gagggtaata aacgtgtttc aagaagcatt ttattagggg atatcactca
     4201 aaagtatgaa gcagcatgtg ataaaaagaa aaaagagcaa gcattagcac aaaaaatgga
     4261 ggaatttacc gcttgttatg aggacatgaa caatattttt gtggatcaag agaaacgttt
     4321 aacagataaa aacatgaaaa aactcactcg tttttatcaa aacttaccag aaaaacagca
     4381 aaaagaggta caagaaaaat atgagcaagt aaaaaaagac gtgcaatatt ttaaagatac
     4441 gcagacagaa gaagcttgtt gtaacttatt tagcgataca atcccttttt caaccagtga
     4501 acaagaacga aaagaacggc aacaatcggt cacatacgaa cgttatgaaa cactgcttca
     4561 gcaagcaaca aacatacaaa atccaacgaa aaaagaaacg gcattaaatt atctagaaag
     4621 tgtcaaggaa tggttggatc agcaacaaca aaattgaatt tgattagcaa tcatgttaaa
     4681 ctgaacctgc aaatgatcaa acattagata cacgaaagcc ccagagaggt cggattctct
     4741 ggggcttttt ttcaggttga tgtcgccttt attgatttta cttcttgttt cggttattca
     4801 accaataact gaataaagag acacaaactt ctacaagaag ggggcaaatg aaccgttcaa
     4861 acattagata cacctccttc cagtcgcaag actgtcggat aggcgacaag ttattatacc
     4921 ttacaaaata aaatctgtgt ggtgttttta agtatccctt attttcgggt tgttagggct
     4981 tgccctgacc gcctgtaagg cgctttaaga acaagataag gttttttacc caagactgtc
     5041 ttaaaacgac ttagacgagc tatcagaggc ttcccccacc tccgttactg acttgttttt
     5101 agcaaaggag cctttaggcg actgcgcagt acaatgtgag agcatagcga tcacactttt
     5161 ttagcgaccg tagggagcaa ggttttgaat tggcagtttt gaaaaatgcg cgaacctgtt
     5221 ggtagaaaaa agaagtggtt tttaggcggg aaattttgtc accctctttt ataatctctt
     5281 tttaaacctt tttttaaacc tctttaccgt cggctgtgtc cttactcctt caatggatac
     5341 agcgttttat aaactacatt ttgtctgctt ttaaactaca ttttgtctgc ttttaaacta
     5401 cattttgtct gcttttaaac tacattttgt ctgcttttaa actctttttt tgcactcacg
     5461 tatgttataa tatgcattat cacagacaaa acgttgttta taacgtctgt atccattgaa
     5521 ggagtaagga ggaatatctt tggcaaatga aatcgtgaaa taccatcatg aattaaatac
     5581 cattccgttg cggaaattta caccagttga aatgaatcta tttttctcta ttgtgtctag
     5641 aatgcgcgaa aaaggcgata aaacagtccg tcttactttt gaccaactaa aagatttaag
     5701 taattataaa gcaactgcta ataatcgttt cattaatgat ttagaaatga cttatgataa
     5761 attaatggat ttacgttttg gtaaaagaag tgctaatggg ctcgatatag atagattcgt
     5821 tatgtttagt gagtttaaaa tacgtggaac tgctaaagat ccatatgtag acatacaagt
     5881 ttacgaaaaa gctttacctt tactaaacaa tttagatgag tgggttcgct attcactcca
     5941 acaatttaac gaattacaaa gtagctatgc aaagactatg tttcgattac taaaacaata
     6001 tcgaactaaa ggttttgctt atttttctaa agaggatttt catgaactat tagatattcc
     6061 aaaaagttac tggaacaacc aagcaaacgt tgataatcgt gttataaagc ctatacgaca
     6121 ggaattaacc gcaatcttca aaggattatc aatcaaaaag aaatacggaa aagggcgagg
     6181 aaaacctgtc gttggctatc aatttacgtt taaaccagaa atcaaaaaag cagatgattt
     6241 taataaacat gcaccaacca aagaagaaca aaataaagca ttggctttag ctgataaaca
     6301 aaa
//
