LOCUS       NZ_CP035645             6303 bp    DNA     circular CON 17-JUN-2024
DEFINITION  Enterococcus faecium strain UAMSEF_01 plasmid unnamed3, complete
            sequence.
ACCESSION   NZ_CP035645
VERSION     NZ_CP035645.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN10863479
            Assembly: GCF_005886545.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6303)
  AUTHORS   Udaondo,Z., Abram,K., Kothari,A. and Jun,S.R.
  TITLE     Top-Down Genomic Surveillance Approach To Investigate the Genomic
            Epidemiology and Antibiotic Resistance Patterns of Enterococcus
            faecium Detected in Cancer Patients in Arkansas
  JOURNAL   Microbiol Spectr 11 (3), e0490122 (2023)
   PUBMED   36995227
REFERENCE   2  (bases 1 to 6303)
  AUTHORS   Jenjaroenpun,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2019) Biomedical Informatics, University of
            Arkansas for Medical Sciences, 4301 W Markham St, Little Rock, AR
            72205, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: JAN-2019
            Assembly Method                   :: Unicycler v. 0.4.4
            Assembly Name                     :: UAMS_Efae_0.4.4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 518.0x
            Sequencing Technology :: Oxford Nanopore MiniION; Illumina NextSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_005886545.1-RS_2024_06_17
            Annotation Date                   :: 06/17/2024 10:53:44
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,102
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,798
            CDSs (with protein)               :: 2,798
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 120 of 213
            Pseudo Genes (incomplete)         :: 90 of 213
            Pseudo Genes (internal stop)      :: 44 of 213
            Pseudo Genes (multiple problems)  :: 34 of 213
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP035645.1.
            Bacteria and source DNA available from Tip's lab.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="UAMSEF_01"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="unnamed3"
                     /geo_loc_name="USA:Arkansas"
                     /collection_date="2018-06-28"
     gene            complement(join(6104..6303,1..334))
                     /locus_tag="EWM53_RS00225"
                     /old_locus_tag="EWM53_00225"
     CDS             complement(join(6104..6303,1..334))
                     /locus_tag="EWM53_RS00225"
                     /old_locus_tag="EWM53_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_002321384.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKVV
                     LRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLREQL
                     ALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATETID
                     KQIDTEKKWWQLWK"
                     /name="DUF536 domain-containing protein"
     gene            complement(355..1305)
                     /locus_tag="EWM53_RS00230"
                     /old_locus_tag="EWM53_00230"
     CDS             complement(355..1305)
                     /locus_tag="EWM53_RS00230"
                     /old_locus_tag="EWM53_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_033642128.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVRL
                     TFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIRGT
                     AKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFAYFS
                     KEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVVGYQF
                     TFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELELLQQI
                     KQLEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ"
                     /name="replication initiation protein"
     gene            1981..2067
                     /locus_tag="EWM53_RS00235"
                     /old_locus_tag="EWM53_00235"
     CDS             1981..2067
                     /locus_tag="EWM53_RS00235"
                     /old_locus_tag="EWM53_00235"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_107532029.1"
                     /translation="MFERFICPLLVEVCVSLFSYWLNNRNKK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(2188..2877)
                     /locus_tag="EWM53_RS00240"
                     /old_locus_tag="EWM53_00240"
     CDS             complement(2188..2877)
                     /locus_tag="EWM53_RS00240"
                     /old_locus_tag="EWM53_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002321377.1"
                     /translation="MAFYLPYLLISASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLVG
                     VIFALSIVLSGLFGVLEGNKRVSRSILLGDITQKYEAACDKKKKEQALAQKMEEFTACY
                     EDMNNIFVDQEKRLTDKNMKKLTRFYQNLPEKQQKEVQEKYEQVKKDVQYFKDTQTEEA
                     CCNLFSDTIPFSTSEQERKERQQSVTYERYETLLQQATNIQNPTKKETALNYLESVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     gene            complement(3122..4036)
                     /locus_tag="EWM53_RS00250"
                     /old_locus_tag="EWM53_00250"
     CDS             complement(3122..4036)
                     /locus_tag="EWM53_RS00250"
                     /old_locus_tag="EWM53_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_002321380.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIRT
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPNHQIAIYTHTDKDHIHNHI
                     VINSVNLETGKKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKTEKRTMSEIKLKEK
                     NEPVWKDEIRFAIDQTMKNKKTRTYTQFCDSLKTFGIHCLNRGKNFTYELVHKKKKVRS
                     NKLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTFTPEQSEI
                     RPTRRNHEQHVERGTDHQQELSL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(4018..4398)
                     /locus_tag="EWM53_RS00255"
                     /old_locus_tag="EWM53_00255"
     CDS             complement(4018..4398)
                     /locus_tag="EWM53_RS00255"
                     /old_locus_tag="EWM53_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_002321381.1"
                     /translation="MSEQHQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPDDA
                     QRLSENLKIMQKELQKIWQQLK"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(4720..4923)
                     /locus_tag="EWM53_RS00260"
                     /old_locus_tag="EWM53_00260"
     CDS             complement(4720..4923)
                     /locus_tag="EWM53_RS00260"
                     /old_locus_tag="EWM53_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002321382.1"
                     /translation="MFLISKSLKSLKTFTKKNPQIATIRIFIFAIAVAIIVNMLSGGQL
                     YKSGLFLVLILVVLDIIKSKKK"
                     /name="hypothetical protein"
     gene            complement(4935..5468)
                     /locus_tag="EWM53_RS00265"
                     /old_locus_tag="EWM53_00265"
     CDS             complement(4935..5468)
                     /locus_tag="EWM53_RS00265"
                     /old_locus_tag="EWM53_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002334266.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENVV
                     IEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAINAA
                     FWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQRGY
                     DAAGRVVATRYSTL"
                     /name="hypothetical protein"
     gene            complement(5918..6088)
                     /locus_tag="EWM53_RS16300"
     CDS             complement(5918..6088)
                     /locus_tag="EWM53_RS16300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002292472.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNSP
                     NEELFVKETDK"
                     /name="hypothetical protein"
     Typing          complement(356..1304)
                     /name="rep_cluster_1197"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.953"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(3126..4036)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.079"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aattattttg ctctctgagg gcaagttgtt ctcgtaaaaa ttcattttct tcacgcaact
       61 gatctagcaa cttgtttgta accacgtttt tgcttggtat attaacattt ttatcgttgt
      121 cttgttcgta ctcgtgcaac ctatctagca acatttggtt gcctaagttg cgagcaagtt
      181 gcaatactcc agaacgcaat acaaccttat aaccacgctc atttttagct atcatggaag
      241 aatctaattt ttggatatgt ttcgatattg cttgcctact catcttaaaa agttctgaca
      301 attcctttat tgtaaaggtt tcttgtggtt gcatactggt tgcctcctag gttgctactg
      361 gaataaatta tctaaatcag ataataaact ttttttctct tttttcgaaa gtgcggactc
      421 tgtttgtttt tcaagagctt ctttctcttt ttctagttgt ttgatttgtt ggaggagttc
      481 aagttctcgt ttcttctgtt tgattttttc gttaattgtt gtagcaggtt cttcgtattc
      541 tttttgttta tcagctaaag ccaatgcttt attttgttct tctttggttg gtgcatgttt
      601 attaaaatca tctgcttttt tgatttctgg tttaaacgta aattgatagc caacgacagg
      661 ttttcctcgc ccttttccgt atttcttttt gattgataat cctttgaaga ttgcggttaa
      721 ttcctgtcgt ataggcttta taacacgatt atcaacgttt gcttggttgt tccagtaact
      781 ttttggaata tctaatagtt catgaaaatc ctctttagaa aaataagcaa aacctttagt
      841 tcgatattgt tttagtaatc gaaacatagt ctttgcatag ctactttgta attcgttaaa
      901 ttgttggagt gaatagcgaa cccactcatc taaattgttt agtaaaggta aagctttttc
      961 gtaaacttgt atgtctacat atggatcttt agcagttcca cgtattttaa actcactaaa
     1021 cataacgaat ctatctatat cgagcccatt agcacttctt ttaccaaaac gtaaatccat
     1081 taatttatca taagtcattt ctaaatcatt aatgaaacga ttattagcag ttgctttata
     1141 attacttaaa tcttttagtt ggtcaaaagt aagacggact gttttatcgc ctttttcgcg
     1201 cattctagac acaatagaga aaaatagatt catttcaact ggtgtaaatt tccgcaacgg
     1261 aatggtattt aattcatgat ggtatttcac gatttcattt gccaaagata ttcctcctta
     1321 ctccttcaat ggatacagac gttataaaca acgttttgtc tgtgataatg catattataa
     1381 catacgtgag tgcaaaaaaa gagtttaaaa gcagacaaaa tgtagtttaa aagcagacaa
     1441 aatgtagttt aaaagcagac aaaatgtagt ttaaaagcag acaaaatgta gtttataaaa
     1501 cgctgtatcc attgaaggag taaggacaca gccgacggta aagaggttta aaaaaaggtt
     1561 taaaaagaga ttataaaaga gggtgacaaa atttcccgcc taaaaaccac ttcttttttc
     1621 taccaacagg ttcgcgcatt tttcaaaact gccaattcaa aaccttgctc cctacggtcg
     1681 ctaaaaaagt gtgatcgcta tgctctcaca ttgtactgcg cagtcgccta aaggctcctt
     1741 tgctaaaaac aagtcagtaa cggaggtggg ggaagcctct gatagctcgt ctaagtcgtt
     1801 ttaagacagt cttgggtaaa aaaccttatc ttgttcttaa agcgccttac aggcggtcag
     1861 ggcaagccct aacaacccga aaataaggga tacttaaaaa caccacacag attttatttt
     1921 gtaaggtata ataacttgtc gcctatccga cagtcttgcg actggaagga ggtgtatcta
     1981 atgtttgaac ggttcatttg cccccttctt gtagaagttt gtgtctcttt attcagttat
     2041 tggttgaata accgaaacaa gaagtaaaat caataaaggc gacatcaacc tgaaaaaaag
     2101 ccccagagaa tccgacctct ctggggcttt cgtgtatcta atgtttgatc atttgcaggt
     2161 tcagtttaac atgattgcta atcaaattca attttgttgt tgctgatcca accattcctt
     2221 gacactttct agataattta atgccgtttc ttttttcgtt ggattttgta tgtttgttgc
     2281 ttgctgaagc agtgtttcat aacgttcgta tgtgaccgat tgttgccgtt cttttcgttc
     2341 ttgttcactg gttgaaaaag ggattgtatc gctaaataag ttacaacaag cttcttctgt
     2401 ctgcgtatct ttaaaatatt gcacgtcttt ttttacttgc tcatattttt cttgtacctc
     2461 tttttgctgt ttttctggta agttttgata aaaacgagtg agttttttca tgtttttatc
     2521 tgttaaacgt ttctcttgat ccacaaaaat attgttcatg tcctcataac aagcggtaaa
     2581 ttcctccatt ttttgtgcta atgcttgctc ttttttcttt ttatcacatg ctgcttcata
     2641 cttttgagtg atatccccta ataaaatgct tcttgaaaca cgtttattac cctctaaaac
     2701 gccgaataaa ccacttaaaa cgattgataa ggcaaatata accccgacca ataaaaatcg
     2761 cttaaatcga attttagagc cttttaatga atatcttgtt tgataagcct tatagctaag
     2821 ccaaataatt cctaaaatac tggcagaaat gagtagatag ggtaaataga atgccatgtc
     2881 tgtttattct ccccatacca accaaaaggc taataatgtt agtactaagc aacataaggg
     2941 ggcaagaata tccatcgatc gttgaattaa agcctttttt tgttcttggt ttcgctggtc
     3001 taatagcgct tggtggagtt ttttgacttg tgaatccatc gtagtttgta tcctttcttg
     3061 ggtataaggc gcaagaaagg cacgttgtct ctttatttcg ttttcgatgt cttttaacgt
     3121 tttagagcga taattcttgt tggtggtctg ttcctcgttc aacgtgttgt tcatggtttc
     3181 ttcgagtcgg tctaatttcc gattgttcag gcgtaaaagt tcgttgttct cttcgtactt
     3241 cttctgcagt ttcgcgaatg ctagctcgag ctcttcgtag ctcgttggtt cgttgttgat
     3301 tgtaggttcg ttctcgtctg tccagctcat gtaaaatcgt ctccttttcg taatcctttc
     3361 ctagtttgtt ggatcgtact tttttctttt tgtggaccaa ttcgtaagta aaattttttc
     3421 cgcgattcaa gcaatgaatg ccgaatgttt taagtgaatc acaaaattgg gtatacgttc
     3481 gagttttttt gtttttcatt gtttgatcaa tcgcaaaccg aatttcgtct ttccaaaccg
     3541 gttcattttt ttcttttaat ttaatttcag acatggttcg tttttcggtt ttctttcctc
     3601 gttccgtgat tgttaagtca tgttcaagac aaatctcgtc atttaggtca taacatttgt
     3661 ttaaaaatgc ttgcccatgg gcttgaaatt ttttcccagt ttcaagatta acggagttaa
     3721 tgacaatgtg attatgaata tggtctttgt ctgtatgtgt gtaaatcgcg atttggtggt
     3781 taggggctat ttttttcgca tactctgccc ctagttgatt ggctttttca gcggtaattt
     3841 ctcctggtcg aaaggactga atcaataagt gtgcttgtac acgatcgtct tttccataaa
     3901 gcgtgcgtat ttgtttcatt tcacttttgg cataatcaat gtcacagttt attcctgttt
     3961 tgacggttgc tcgtggttct gcgtaattga ttgctcggct acatgaagtg gttcgactta
     4021 ctttaactgt tgccatatct tttgtagctc cttttgcatg atttttaggt tttcggataa
     4081 acgttgagca tcatcaggag caacgtcgtg ttggtgtgta ttgcaccact tggcaatttg
     4141 attgatattc cctccagctt tggctaaatc gcgcgcgatt gcctgggcaa tgtctggcgc
     4201 cactttcggt gtcaccagac gcgcgttttg tgccttggct ttcacgaaag ccggcacact
     4261 catttgcaaa gcttcagctg actgcttcag ctttgcaaat tcggattcgc tcactcgaaa
     4321 actgatttgt tttggctcgg atcgataggt atatttttta gaatgatcgc tagccaaatt
     4381 ttgatgttgt tcgctcataa gtcccaccct ttcagtacgt cctacggacg tatcgttcgc
     4441 aataccattg tacctaaaaa ggtacaatga gtaaagctca ctaaaagggt ttggcaagct
     4501 ctatatttgc tagccaaata cctccgtatt tggtgcaaat aaccgcttgt caaaggagcg
     4561 caagctcccc cttgaatccc cctcaaaatc cacgctccac aatacaaaaa aaggctagca
     4621 tttgctagcc actttttgta aagtttcgct tatttcaccg aaggtgaaat tgttgctttg
     4681 taatattcaa acaaccctat tcttctaaaa tgtatcgatt tatttttttt tagactttat
     4741 tatgtctaag acaactaaga ttaacactaa aaataaacca gatttgtata attgccctcc
     4801 gcttagcata ttaacaatta tagcaacagc tatagcaaat ataaaaatac gaatagtagc
     4861 tatttgcgga ttctttttag taaatgtttt taatgatttt aatgatttag atatcaaaaa
     4921 catcactcct taacttataa cgttgaatat ctagtagcta caactcttcc agctgcatca
     4981 tatccccttt ggtaataaaa tccaatccag ccattaccat ttttgtccca ttcttttgca
     5041 actgcattcc ctggagaagc acctttttta gtccaacttg cacctgttaa caaagcacca
     5101 attgcccaac ctgggatacc aaacatagaa gtgctagctt tccctcctgc ccaaaaggca
     5161 gcattaatag cattagcagc tactccagtg gttacagcaa tatttgtata ttcctttttg
     5221 ccccatcttg caactgcagc tcttgtcatg atatctgaag aatttgcaaa atcttccgca
     5281 ctataaccat cctcatattt agttacgacc ataccatctt cagtgtactc aataactaca
     5341 ttttcagtag cattgttttc gtttaatgat accacttggg aattttctga agcataaact
     5401 atagtagttg acggtaaaac aactgcccca atagttaaca aagctacccc taaagtaaat
     5461 tttttcatag aatttcctcc tgatacagaa aaatatatag aaaacgtttt ctttgtgaaa
     5521 atcataaaac aaaaggtata ggcttgtcaa tattttattt agtaaagata atttatattt
     5581 ttgataatgt ggtatagtta gaatgcatta ggaaaatatg ttatattaaa ttcttgttga
     5641 cataatgtac actatgccaa gtagcccttg tatcaaccga tacagaggct cactttgtga
     5701 ttttcgattg gtctaggtca aaagctcttt ttaagcttga ttttctgcgg ttgcttcttt
     5761 ccgtttcttt gtgaattttt accagtcatt ttttactggg ttgttatttt taataaaata
     5821 tgataaattt ttggtagcgc ttcataagcg cgttgctttt tcaagaataa acactcatta
     5881 ttttttaact ggctgctcga aagagcagtc ttttttatta tttatctgtt tcttttacaa
     5941 acaattcttc gttaggactg ttgtaataat ttggattcaa tttcaattca ccatgtttat
     6001 tccctttatt gatcactgac aataacatcg gactgaatcc tagaattcct agtaaaatag
     6061 aaccgaatag aagatttttt cttgtcatga atgttccctc cagttatttc caaagttgcc
     6121 accatttttt ttcagtatca atttgtttgt ctattgtttc tgttgcttca gaaagagtcg
     6181 ttgagtcatt ctctggagct tcttctgtat aagtcaactg tagtttttct tgcagttttt
     6241 cgatttgttt gttggcttgt agggtgagtt gttgttgctg gtcgagtagt ttttgtatat
     6301 gag
//
