LOCUS       NZ_CP035644            37314 bp    DNA     circular CON 17-JUN-2024
DEFINITION  Enterococcus faecium strain UAMSEF_01 plasmid unnamed2, complete
            sequence.
ACCESSION   NZ_CP035644
VERSION     NZ_CP035644.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN10863479
            Assembly: GCF_005886545.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37314)
  AUTHORS   Udaondo,Z., Abram,K., Kothari,A. and Jun,S.R.
  TITLE     Top-Down Genomic Surveillance Approach To Investigate the Genomic
            Epidemiology and Antibiotic Resistance Patterns of Enterococcus
            faecium Detected in Cancer Patients in Arkansas
  JOURNAL   Microbiol Spectr 11 (3), e0490122 (2023)
   PUBMED   36995227
REFERENCE   2  (bases 1 to 37314)
  AUTHORS   Jenjaroenpun,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2019) Biomedical Informatics, University of
            Arkansas for Medical Sciences, 4301 W Markham St, Little Rock, AR
            72205, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: JAN-2019
            Assembly Method                   :: Unicycler v. 0.4.4
            Assembly Name                     :: UAMS_Efae_0.4.4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 518.0x
            Sequencing Technology :: Oxford Nanopore MiniION; Illumina NextSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_005886545.1-RS_2024_06_17
            Annotation Date                   :: 06/17/2024 10:53:44
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,102
            CDSs (total)                      :: 3,011
            Genes (coding)                    :: 2,798
            CDSs (with protein)               :: 2,798
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 120 of 213
            Pseudo Genes (incomplete)         :: 90 of 213
            Pseudo Genes (internal stop)      :: 44 of 213
            Pseudo Genes (multiple problems)  :: 34 of 213
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP035644.1.
            Bacteria and source DNA available from Tip's lab.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..37314
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="UAMSEF_01"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="unnamed2"
                     /geo_loc_name="USA:Arkansas"
                     /collection_date="2018-06-28"
     gene            1..1041
                     /locus_tag="EWM53_RS00005"
                     /old_locus_tag="EWM53_00005"
     CDS             1..1041
                     /locus_tag="EWM53_RS00005"
                     /old_locus_tag="EWM53_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="WP_000997689.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLLK
                     DRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGRVN
                     KRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTTVIA
                     KIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVDIMKE
                     DSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELESYGIDA
                     DKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFNELPTVS
                     TENWLE"
                     /name="replication initiator protein A"
     gene            complement(1657..>1746)
                     /locus_tag="EWM53_RS00010"
                     /old_locus_tag="EWM53_00010"
                     /pseudo=""
     CDS             complement(1657..>1746)
                     /locus_tag="EWM53_RS00010"
                     /old_locus_tag="EWM53_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125701.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /name="IS6 family transposase"
     gene            complement(1827..3119)
                     /locus_tag="EWM53_RS00015"
                     /old_locus_tag="EWM53_00015"
     CDS             complement(1827..3119)
                     /locus_tag="EWM53_RS00015"
                     /old_locus_tag="EWM53_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628567.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1251 family transposase"
                     /protein_id="WP_000122610.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIYT
                     AQECIHCKHQIASDIVRWGTTTVRLLMNDVSEYRTYLELKKQRFKCKACQRTFVADTSV
                     AKKHCFISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTPLPK
                     VLCFDEFKSVRGVSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREAVEWI
                     VIDMYAPYVSLVKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQKHYQLG
                     KQLKRYWKLLQKDERKLDYTRRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQLYQDFL
                     YAVKERDYVSFEELLTNNIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPLECLNNHI
                     KVLKRNAYGFRSFYNFKLRIMIRHGNALIFN"
                     /name="ISL3-like element IS1251 family transposase"
     gene            3467..4009
                     /locus_tag="EWM53_RS00025"
                     /old_locus_tag="EWM53_00025"
     CDS             3467..4009
                     /locus_tag="EWM53_RS00025"
                     /old_locus_tag="EWM53_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="WP_002321977.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLEE
                     AGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAMYK
                     IKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPASGG
                     QEPESRMIEPVLLQSFK"
                     /name="HTH domain-containing protein"
     gene            complement(4039..4719)
                     /locus_tag="EWM53_RS00030"
                     /old_locus_tag="EWM53_00030"
     CDS             complement(4039..4719)
                     /locus_tag="EWM53_RS00030"
                     /old_locus_tag="EWM53_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="WP_001015311.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHT
                     TIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWL
                     RKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLN
                     NLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLL
                     GIPA"
                     /name="IS6-like element IS1216 family transposase"
     gene            complement(<4795..4887)
                     /locus_tag="EWM53_RS16510"
                     /old_locus_tag="EWM53_00035"
                     /pseudo=""
     CDS             complement(<4795..4887)
                     /locus_tag="EWM53_RS16510"
                     /old_locus_tag="EWM53_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            6036..7055
                     /locus_tag="EWM53_RS00040"
                     /old_locus_tag="EWM53_00040"
     CDS             6036..7055
                     /locus_tag="EWM53_RS00040"
                     /old_locus_tag="EWM53_00040"
                     /inference="COORDINATES: protein motif:HMM:NF019371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="WP_010729551.1"
                     /translation="MKTSNRKYKVKTVNHRIPPKKALCVINNSKVINQELKPYLSEQEM
                     IKKISKDIFLTNSDTILLEKFLKKNSYFRFSIYVKLMKNIDKVTILDVIETYKLDSFIR
                     NQLHYFVNQIEIFWKKSLSDNMCVSYEETSIFPKNQCYLDKNIYSDLKWAEDIIGHFNS
                     FFYSNQSPTFKHHHIKKNHCLPIWALFEEITFGSLTTFINQLNTEYYNNWVMSCYDNPK
                     YKKAIRSWANVIRAYRNKIAHHSRIYAFKVTDAPTIIKNDSKSYFPENNLKETQKLYLY
                     GGLYVIKHLLIYEDSMTQTLWNKFLIDLEKKIDKIPSLEKSNYGFPENWRNKLKIMIV"
                     /name="Abi family protein"
     gene            complement(7557..7973)
                     /locus_tag="EWM53_RS00045"
                     /old_locus_tag="EWM53_00045"
     CDS             complement(7557..7973)
                     /locus_tag="EWM53_RS00045"
                     /old_locus_tag="EWM53_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002305824.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSYE
                     PDTNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKIEN
                     QLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
                     /name="hypothetical protein"
     gene            complement(7975..8256)
                     /locus_tag="EWM53_RS00050"
                     /old_locus_tag="EWM53_00050"
     CDS             complement(7975..8256)
                     /locus_tag="EWM53_RS00050"
                     /old_locus_tag="EWM53_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000353845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000353845.1"
                     /translation="MDILVRNINPKIITRIDKRCQELTTKTGKKWSRNQYLKILIENDF
                     DHELMNYKKDQFDLLFEKFIAIQAENTNILNDYVHTNNQLIETLIGGI"
                     /name="hypothetical protein"
     gene            complement(8632..9156)
                     /locus_tag="EWM53_RS00055"
                     /old_locus_tag="EWM53_00055"
     CDS             complement(8632..9156)
                     /locus_tag="EWM53_RS00055"
                     /old_locus_tag="EWM53_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_002305828.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKIGNKFVINET
                     GQKAIKSMFFNENENSNQQLVGEKTKTENHKNANQVPELEVLSLLEQQLNQKDMQLQEK
                     DEQIKQMQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIEKQS
                     KTEKKWWQFWQ"
                     /name="DUF536 domain-containing protein"
     gene            complement(9173..>10003)
                     /locus_tag="EWM53_RS00060"
                     /old_locus_tag="EWM53_00060"
                     /pseudo=""
     CDS             complement(9173..>10003)
                     /locus_tag="EWM53_RS00060"
                     /old_locus_tag="EWM53_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332718.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            complement(10059..10738)
                     /locus_tag="EWM53_RS00065"
                     /old_locus_tag="EWM53_00065"
     CDS             complement(join(10059..10552,10552..10738))
                     /locus_tag="EWM53_RS00065"
                     /old_locus_tag="EWM53_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="WP_001015311.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHT
                     TIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWL
                     RKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLN
                     NLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLL
                     GIPA"
                     /name="IS6-like element IS1216 family transposase"
     gene            <10813..10926
                     /locus_tag="EWM53_RS00070"
                     /old_locus_tag="EWM53_00070"
                     /pseudo=""
     CDS             <10813..10926
                     /locus_tag="EWM53_RS00070"
                     /old_locus_tag="EWM53_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /name="helix-turn-helix domain-containing protein"
     gene            11140..11835
                     /gene="vanR-A"
                     /locus_tag="EWM53_RS00075"
                     /old_locus_tag="EWM53_00075"
     ARG             11141..11832
                     /name="vanR-A"
                     /gene="vanR-A"
                     /gene_name="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /tool="amrfinderplus"
                     /drug_class="GLYCOPEPTIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            11813..12967
                     /locus_tag="EWM53_RS00080"
                     /old_locus_tag="EWM53_00080"
     CDS             11813..12967
                     /locus_tag="EWM53_RS00080"
                     /old_locus_tag="EWM53_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="WP_002305818.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGKL
                     GDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKYFD
                     EINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVMYLA
                     HDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRYNLQT
                     ITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFNNILKN
                     AAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSSDTGGAG
                     LGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
                     /name="vancomycin resistance histidine kinase VanS-A"
     gene            complement(13065..14358)
                     /locus_tag="EWM53_RS00085"
                     /old_locus_tag="EWM53_00085"
                     /pseudo=""
     CDS             complement(13065..14358)
                     /locus_tag="EWM53_RS00085"
                     /old_locus_tag="EWM53_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628567.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1251 family transposase"
                     /name="ISL3-like element IS1251 family transposase"
     gene            14690..15658
                     /locus_tag="EWM53_RS00090"
                     /old_locus_tag="EWM53_00090"
     CDS             14690..15658
                     /locus_tag="EWM53_RS00090"
                     /old_locus_tag="EWM53_00090"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="WP_001059542.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKSA
                     PFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAYSP
                     DSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKAVIE
                     RLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQRMKQ
                     GAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQFLLKL
                     QRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
                     /name="D-lactate dehydrogenase VanH-A"
     ARG             15634..16682
                     /name="vanA"
                     /gene="vanA"
                     /gene_name="glycopeptide resistance gene cluster; Van
                     ligase"
                     /tool="rgi"
                     /drug_class="glycopeptide antibiotic"
                     /identity="99.71"
                     /coverage="101.75"
     ARG             15652..16679
                     /name="vanA"
                     /gene="vanA"
                     /gene_name="D-alanine--(R)-lactate ligase VanA"
                     /tool="amrfinderplus"
                     /drug_class="GLYCOPEPTIDE"
                     /identity="99.71"
                     /coverage="100.0"
     gene            16688..17296
                     /locus_tag="EWM53_RS00100"
                     /old_locus_tag="EWM53_00100"
     CDS             16688..17296
                     /locus_tag="EWM53_RS00100"
                     /old_locus_tag="EWM53_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="WP_000402348.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTYE
                     LAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRTEM
                     ISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEAQNR
                     RRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
                     /name="D-Ala-D-Ala dipeptidase VanX-A"
     gene            17724..18635
                     /locus_tag="EWM53_RS00105"
                     /old_locus_tag="EWM53_00105"
     CDS             17724..18635
                     /locus_tag="EWM53_RS00105"
                     /old_locus_tag="EWM53_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="WP_001812592.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHLE
                     NSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLLDS
                     NIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGYSEH
                     NSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIRYVGL
                     PHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNLRYEIS
                     GNNIDGVIVTVFPGSTHTNSRR"
                     /name="D-Ala-D-Ala carboxypeptidase VanY-A"
     gene            18788..19273
                     /gene="vanZ-A"
                     /locus_tag="EWM53_RS00110"
                     /old_locus_tag="EWM53_00110"
     ARG             18789..19270
                     /name="vanZ-A"
                     /gene="vanZ-A"
                     /gene_name="glycopeptide resistance protein VanZ-A"
                     /tool="amrfinderplus"
                     /drug_class="GLYCOPEPTIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(19560..19889)
                     /locus_tag="EWM53_RS00115"
                     /old_locus_tag="EWM53_00115"
     CDS             complement(19560..19889)
                     /locus_tag="EWM53_RS00115"
                     /old_locus_tag="EWM53_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003700 - DNA-binding transcription factor activity
                     [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="WP_000754864.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIKI
                     FYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDILS
                     AIRVF"
                     /name="metalloregulator ArsR/SmtB family transcription
                     factor"
     gene            20194..20523
                     /locus_tag="EWM53_RS00120"
                     /old_locus_tag="EWM53_00120"
     CDS             20194..20523
                     /locus_tag="EWM53_RS00120"
                     /old_locus_tag="EWM53_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000629052.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEPK
                     KEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEELA
                     AEIGL"
                     /name="hypothetical protein"
     gene            20513..20800
                     /locus_tag="EWM53_RS00125"
                     /old_locus_tag="EWM53_00125"
     CDS             20513..20800
                     /locus_tag="EWM53_RS00125"
                     /old_locus_tag="EWM53_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_000312841.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQK
                     TGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPILLCGTHENFYEQLKKIR"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(21079..21642)
                     /locus_tag="EWM53_RS00130"
                     /old_locus_tag="EWM53_00130"
     CDS             complement(21079..21642)
                     /locus_tag="EWM53_RS00130"
                     /old_locus_tag="EWM53_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_000017403.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAVR
                     PQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTPHG
                     RLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQVYS
                     VKEITEMTGISKATLYRYLKDNRE"
                     /name="recombinase family protein"
     gene            22285..23238
                     /locus_tag="EWM53_RS00135"
                     /old_locus_tag="EWM53_00135"
     CDS             22285..23238
                     /locus_tag="EWM53_RS00135"
                     /old_locus_tag="EWM53_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="WP_000222571.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNFL
                     KCGGTALEYFEQYKENKRRCGRTEIIFPAEEKEYIEKRSTEGWTPDVIIGRAERTFSCS
                     ISTLYRRFKTGEFNVLHLPMQGKRKPNGYKEKRGKQAFKRNISERKKDYVVFEEEFGHL
                     EGDTIVGIHHKSAVITLVERLSKAIIVLKPEGRKAVDIENSINEWLQSVPKNLFKSITF
                     DCGKEFSNWKSISNTNDIDIYFADPGTPSQRGLNEHSNGLLRKDGLPKEMDFNQVNQGF
                     ISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA"
                     /name="IS30 family transposase"
     gene            23216..24691
                     /locus_tag="EWM53_RS00140"
                     /old_locus_tag="EWM53_00140"
     CDS             23216..24691
                     /locus_tag="EWM53_RS00140"
                     /old_locus_tag="EWM53_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="WP_000443455.1"
                     /translation="MESFVSLNLTNNTSKKNQPKDIKDIVDTRMYKERDDLNYTLIINK
                     DGNYSWRPLVLLHPILYVDLVNCLTKEDHWEELKKRFSEFKKDKNINCYSIPVEASKGS
                     KRTDLGETILNWWEYFEQKSLAQNIKYQYCMFTDIADCYPSIYTHSITWAMYGKEKVKN
                     DENKCSGSLGDKIDKKISGMQHNQTNGIPQGSVLMDFVAEIVLGYVDLRLSKELKKLEN
                     LKDYKVLRYRDDYRIFSNNRNDLEQIMKVLSEVLYDLNFKLNSSKTKLSDDIIIDSIKK
                     DKLYWEEIKASIRMNATNLDGDREIRYSINLQKHLLEIKKLADKHPNSGQLKKALSEFN
                     KERIIKITSKPNDLLPLIGILVDIMINNPGSLPQCVLIIGQLLNFENESKAREIVEMII
                     NKYALKPNTEFMMIWFQRLTTIFGEMFDSVLSSEFVSKIKDPVSNQMFSVDWLNEDAKK
                     SFYEGTIKDEEKYIKMSKKIYITEFDDFNDYPF"
                     /name="RNA-directed DNA polymerase"
     gene            25063..26250
                     /locus_tag="EWM53_RS00145"
                     /old_locus_tag="EWM53_00145"
     CDS             25063..26250
                     /locus_tag="EWM53_RS00145"
                     /old_locus_tag="EWM53_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="WP_002296840.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQY
                     IQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKALI
                     AAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGSEYP
                     FFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQSFVQ
                     RGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVKDELKS
                     IFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRRRIRTTN
                     VLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNSNNI"
                     /name="IS256-like element IS16 family transposase"
     gene            26503..>27966
                     /locus_tag="EWM53_RS00150"
                     /old_locus_tag="EWM53_00150"
                     /pseudo=""
     CDS             26503..>27966
                     /locus_tag="EWM53_RS00150"
                     /old_locus_tag="EWM53_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305144.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0009007 - site-specific DNA-methyltransferase
                     (adenine-specific) activity [Evidence IEA]"
                     /GO_process="GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M"
                     /name="type I restriction-modification system subunit M"
     gene            complement(28004..28683)
                     /locus_tag="EWM53_RS00155"
                     /old_locus_tag="EWM53_00155"
     CDS             complement(join(28004..28497,28497..28683))
                     /locus_tag="EWM53_RS00155"
                     /old_locus_tag="EWM53_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="WP_002287920.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHT
                     TIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIGADGLTLDIWL
                     RKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLN
                     NLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLL
                     GIPA"
                     /name="IS6-like element IS1216 family transposase"
     gene            <28759..29325
                     /locus_tag="EWM53_RS00160"
                     /old_locus_tag="EWM53_00160"
                     /pseudo=""
     CDS             <28759..29325
                     /locus_tag="EWM53_RS00160"
                     /old_locus_tag="EWM53_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(29372..29608)
                     /locus_tag="EWM53_RS16770"
     CDS             complement(29372..29608)
                     /locus_tag="EWM53_RS16770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_306297428.1"
                     /translation="MKQNTSIAKNTTKFKCTAAAWRISISPTNRTNNRGMILFIWISFF
                     LLGYRVLPAYFAPPKRRGRLERLVRNKNHSLSY"
                     /name="hypothetical protein"
     gene            29706..29885
                     /locus_tag="EWM53_RS00170"
                     /old_locus_tag="EWM53_00170"
     CDS             29706..29885
                     /locus_tag="EWM53_RS00170"
                     /old_locus_tag="EWM53_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001120991.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLLI
                     AQEKAGFEVPALKK"
                     /name="hypothetical protein"
     gene            30016..30285
                     /locus_tag="EWM53_RS00175"
                     /old_locus_tag="EWM53_00175"
     CDS             30016..30285
                     /locus_tag="EWM53_RS00175"
                     /old_locus_tag="EWM53_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /GO_component="GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="WP_000388479.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKRD
                     YDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
                     /name="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
     gene            30278..30535
                     /locus_tag="EWM53_RS00180"
                     /old_locus_tag="EWM53_00180"
     CDS             30278..30535
                     /locus_tag="EWM53_RS00180"
                     /old_locus_tag="EWM53_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /GO_process="GO:0008219 - cell death [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="WP_000588503.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLGK
                     PEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
                     /name="Txe/YoeB family addiction module toxin"
     gene            30994..31998
                     /locus_tag="EWM53_RS00185"
                     /old_locus_tag="EWM53_00185"
     CDS             30994..31998
                     /locus_tag="EWM53_RS00185"
                     /old_locus_tag="EWM53_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000455809.1"
                     /translation="MEYKFNLNLKEVLPTQGTININGQDNDDDIVIKKIEFIDSDINVF
                     FYSKEKLADFSNIAEMIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACEI
                     GVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRNKL
                     VHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFMYRDFYEESIELTPTEPNKSQITL
                     SKELERKVESQELAIGISITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD"
                     /name="hypothetical protein"
     gene            complement(32163..32777)
                     /locus_tag="EWM53_RS00190"
                     /old_locus_tag="EWM53_00190"
     CDS             complement(32163..32777)
                     /locus_tag="EWM53_RS00190"
                     /old_locus_tag="EWM53_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_001261742.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRPV
                     FQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGNPL
                     LDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQRRL
                     VYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
                     /name="recombinase family protein"
     gene            33227..34552
                     /locus_tag="EWM53_RS00195"
                     /old_locus_tag="EWM53_00195"
     CDS             33227..34552
                     /locus_tag="EWM53_RS00195"
                     /old_locus_tag="EWM53_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="WP_000969590.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRAD
                     NTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNIFR
                     RYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKKLAQMIQENIKEELGLIATVG
                     VGDNPLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRLNQMG
                     IMSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQVLPRD
                     YTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKIPITDNT
                     KELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEENLDKIIDK
                     IRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGNGGIKND"
                     /name="Y-family DNA polymerase"
     gene            34545..34895
                     /locus_tag="EWM53_RS00200"
                     /old_locus_tag="EWM53_00200"
     CDS             34545..34895
                     /locus_tag="EWM53_RS00200"
                     /old_locus_tag="EWM53_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000568378.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTEE
                     IAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIIGKILGSDELGIYVGKDKIHYDEIRHI
                     EFYQDNKWSRLE"
                     /name="hypothetical protein"
     gene            34892..35071
                     /locus_tag="EWM53_RS00205"
                     /old_locus_tag="EWM53_00205"
     CDS             34892..35071
                     /locus_tag="EWM53_RS00205"
                     /old_locus_tag="EWM53_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001021554.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAAA
                     GYHNFRFYIFPLYI"
                     /name="hypothetical protein"
     gene            35207..35497
                     /locus_tag="EWM53_RS00210"
                     /old_locus_tag="EWM53_00210"
     CDS             35207..35497
                     /locus_tag="EWM53_RS00210"
                     /old_locus_tag="EWM53_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000026576.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITRQ
                     RRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
                     /name="hypothetical protein"
     gene            35865..36653
                     /locus_tag="EWM53_RS00215"
                     /old_locus_tag="EWM53_00215"
     CDS             35865..36653
                     /locus_tag="EWM53_RS00215"
                     /old_locus_tag="EWM53_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA]"
                     /GO_process="GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="WP_000101106.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQGN
                     ATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVGSE
                     HRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKLSET
                     IETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKTYIRRTITVD
                     EAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
                     /name="AAA family ATPase"
     gene            36640..36966
                     /locus_tag="EWM53_RS00220"
                     /old_locus_tag="EWM53_00220"
     CDS             36640..36966
                     /locus_tag="EWM53_RS00220"
                     /old_locus_tag="EWM53_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000796719.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKDQ
                     GSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLAKK
                     QNKI"
                     /name="hypothetical protein"
     ARG             14691..15655
                     /name="vanH-A"
                     /gene="vanH-A"
                     /gene_name="D-lactate dehydrogenase VanH-A"
                     /tool="amrfinderplus"
                     /drug_class="GLYCOPEPTIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             16689..17293
                     /name="vanX-A"
                     /gene="vanX-A"
                     /gene_name="D-Ala-D-Ala dipeptidase VanX-A"
                     /tool="amrfinderplus"
                     /drug_class="GLYCOPEPTIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             11814..12964
                     /name="vanS-A"
                     /gene="vanS-A"
                     /gene_name="vancomycin resistance histidine kinase VanS-A"
                     /tool="amrfinderplus"
                     /drug_class="GLYCOPEPTIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             17725..18632
                     /name="vanY-A"
                     /gene="vanY-A"
                     /gene_name="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /tool="amrfinderplus"
                     /drug_class="GLYCOPEPTIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             14691..15658
                     /name="vanH gene in vanA cluster"
                     /gene="vanH gene in vanA cluster"
                     /gene_name="vanH; glycopeptide resistance gene cluster"
                     /tool="rgi"
                     /drug_class="glycopeptide antibiotic"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             11141..11835
                     /name="vanR gene in vanA cluster"
                     /gene="vanR gene in vanA cluster"
                     /gene_name="glycopeptide resistance gene cluster; vanR"
                     /tool="rgi"
                     /drug_class="glycopeptide antibiotic"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             17725..18635
                     /name="vanY gene in vanA cluster"
                     /gene="vanY gene in vanA cluster"
                     /gene_name="vanY; glycopeptide resistance gene cluster"
                     /tool="rgi"
                     /drug_class="glycopeptide antibiotic"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             18789..19273
                     /name="vanZ gene in vanA cluster"
                     /gene="vanZ gene in vanA cluster"
                     /gene_name="vanZ; glycopeptide resistance gene cluster"
                     /tool="rgi"
                     /drug_class="glycopeptide antibiotic"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             11814..12968
                     /name="vanS gene in vanA cluster"
                     /gene="vanS gene in vanA cluster"
                     /gene_name="vanS; glycopeptide resistance gene cluster"
                     /tool="rgi"
                     /drug_class="glycopeptide antibiotic"
                     /identity="100.0"
                     /coverage="94.79"
     ARG             16689..17296
                     /name="vanX gene in vanA cluster"
                     /gene="vanX gene in vanA cluster"
                     /gene_name="vanX; glycopeptide resistance gene cluster"
                     /tool="rgi"
                     /drug_class="glycopeptide antibiotic"
                     /identity="99.5"
                     /coverage="100.0"
     Typing          2..1041
                     /name="rep_cluster_889"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(9178..10005)
                     /name="rep_cluster_1093"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.312"
                     /coverage="95.0"
                     /evalue="0.0"
ORIGIN
        1 atgaatgatt ttaattatta caaatcaaaa gaaatttatc gtgaaaaata ttatcaaatg
       61 cctaaggtat tttttactaa cgaaaaatac atggatctat caaatgatgc aaaaattgct
      121 tatatgctac ttaaagatcg atttgattat tcagtcaaaa ataactgggt agattctgat
      181 gataatatat ttttcatatt cacagttgaa gaattaatga aattgttaca atgccgcgaa
      241 ggtaaagtat ctaaaatcaa aaaggaatta gaaaaagcag gtcttctgaa acagaagaga
      301 ggaagagtaa ataaacgtga cggtaagatt gaatccatgc ctaatttatt gtacttaggc
      361 aagccagaag taacgaatca agacgtcttt aaaatcatgg aagaggaaga caatactgat
      421 accaccgtaa ttgcgaaaat cgcaaatacg gcaaaaccgc gagaaatcaa tgataccacc
      481 gtaattgcga aaatcgcaaa tacggcaaaa tccagtcata tcaaggactc aaccgtaatt
      541 gcgaaaatcg cagacaatct attctattct aattctatag acactaatag acacataata
      601 gacactgaaa ctgatcagtt acaaaatcaa gttttactag ataacttcgt cgacatcatg
      661 aaagaggaca gtataaatac atttgtacca gaaaatgtat taaacttgat aaaaacattt
      721 tctagtactt acgaagacgc tcaaaaaacc gtccaaacta ttcataatgc aaagaaaaaa
      781 gctgaagaat tagagcaaac agttgttgtt tatgaagaac tggaatctta tggaattgat
      841 gctgacaaag gattatatat gactttgctg aaagcttatc aaaaacaaaa aactgaaaaa
      901 gtaaacaatt tgcagaatct aattttcgtt tatgtaaaaa attggtttgt cgaaaaagct
      961 attgcggcaa agcttgcaaa cgaacaaaga gaaacattta acgaattacc aacagttagt
     1021 acggaaaatt ggctagagta gttgataaaa agcgatcact ataaaaatga tcaattttaa
     1081 attagaactt ttttatgatt ttaattttat tacaaatgtt acaaataaat agctattatt
     1141 ccggagtttt tagattaaaa gtttatgaaa acaatcacaa acgtaggttt taaagtcata
     1201 atcgttagat attttagtta tttttcattc aaaaaaatag ggaactagtg tttgcatatg
     1261 tgcttttttg agttataata attcatgaag agaggtaatt cacttatttc cccaagctga
     1321 aaagcctgat attacttctt ttcaggcagt gctgctaata ttcaattaca cttttttgta
     1381 tatttttagt tgttttaaat ttaattgtct gatatgcttg tgatgaattg attttcgttc
     1441 tgcacttttg gacgataaat tgtgataatt gaaaatacaa aaaaaagaaa ggcccctgta
     1501 aggctcggga gactggtaat ctccgtcagt gtcctacagg aaagcattaa gatgacgcct
     1561 taatgcgtgc ccttcagtgt tacggtatta cccgcaacgg acggttctgt tgcaaagttt
     1621 taaataaaga ataaaatccc ttacggtatc tatgatttaa gctgggattc ccaataatac
     1681 cttgatttca gtacagaccg aaaacccgaa gagagtgcct tcttttcggg ttttcttata
     1741 taatccggct ctttgtcaaa tgatgttggt aaagagaaat cttagaataa ttggagatca
     1801 agtggatcag ttcctcttga tcttttttag ttgaaaatca atgcgttgcc atgacgaatc
     1861 atgatgcgca atttgaaatt ataaaagcta cgaaagccat atgcatttcg ttttaacact
     1921 ttgatatggt tgttcagaca ttcaagtggg ccatttgaat aactttgttg taaagcattt
     1981 ttgatttgtg gcagaaattt ctggaacgtt ctcaaggtcg tctgatagcc ttcgggaagc
     2041 atgatattat tggtaagtag ttcttcaaac gatacgtagt ctcgttcctt tacagcgtaa
     2101 agaaagtctt gatataactg atagcctaca cgcaaagcag ggctcccttt cagtaagcga
     2161 tccacaatat cggtctctgt taaatgagct ttgaagccag gtcgccaaag gcgtcgagta
     2221 taatcaagct tgcgttcatc tttttgaaga agtttccagt atctttttaa ttgcttacct
     2281 aattgatagt gtttttgttc tttgcttttt aatagttggt tcgtttcttt gattcgatga
     2341 ttgcgaaagg tacgaccaat atgttggacg atgtgaaacc ggtcaataat cagctgtgct
     2401 tttggaaaca gctttttaac gagcgataca taaggagcgt acatatcaat cacgatccat
     2461 tcgaccgctt cacgaatttc tcgtgaaaaa tgactaaaat aacgttttaa aaaaggcagt
     2521 tgtcggttct cgacaatgtc caataagcgc tgtgtttgtc catccatcat gatgaaactc
     2581 atagcacctg aaacaccccg aactgatttg aattcatcaa aacaaagtac cttaggtaac
     2641 ggtgtccgaa aaggattcaa cggacgataa aatcgcttca taatacggta aacagaagag
     2701 accgatagat ttttctgagc ggcgatctct gtcattgagg tatttttttt cagacgagtc
     2761 acgacagacc agcggacttt ctcactgatg aaacaatgtt ttttcgcaac agaagtatct
     2821 gcgacaaagg tccgctgaca agctttgcat ttgaagcgtt gctttttcaa ttcgaggtag
     2881 gtacggtatt cagaaacatc gttcattaac aaccggacgg ttgttgtacc ccaacgcaca
     2941 atatctgaag caatctggtg tttacagtgg atgcattctt gggctgtata aatcaagttt
     3001 ccacgataaa tatgacagat ttgccctttg tatttttctt ttgtgagaca attttcgtta
     3061 aaagtaactg ataagtctag aatatctaat gtttctttga taaggtgagt gtaagacatc
     3121 ttgattcctc cttttgggtt ggttcgccaa attaattcta aaggatttca aggtgtcttt
     3181 tctatatgtt ttggaaaata aaaaagatgc tggtgtcatc caccaacatc aaaaagtata
     3241 gagcctcttt tctgaagtac atccgcaact gtccatactc tgatgtttta tatcttttct
     3301 aaaagttcgc tagatagagt tctatattag agatgtaaag agttttggtg aagaggtttg
     3361 tgagtatggc ttttataacc agggtgcaat gagaagcact aacagcagct agcttaagaa
     3421 cgcataggat atacttttta tggaagtcac agaggaggaa taaaatttga acaggattaa
     3481 cagagtaacc tctattctta ttcagctgca atcaaaaaaa ataattcctg ccaaagaaat
     3541 tgcacagcga tttaatataa gcttaaggac agtttacaga gatatccgga cacttgaaga
     3601 agccggaata ccaattggat ctgaggccgg aaaaggatat tttcttgtag agggcttcct
     3661 acttccgccg gtaatgttta cagcggcgga agtgggtgca ttgattacag cagggaaatt
     3721 tttaaattgc catggagatg aatcatttat aaaggatttt gattcagcta tgtataaaat
     3781 caaatctatt ttgaagcatg gtgaaaaaaa ctatgcacag gagttggaga acagtattaa
     3841 cgtgtacagc acatctggac agaaaaatac attggcggat aacgttattg cagcaattca
     3901 gactgcaatc tgcaataaaa gggtaatatc aattcaatat cctgcatcag gagggcaaga
     3961 accggaaagc agaatgatag agccggttct gttgcaaagt tttaaataaa gaataaaatc
     4021 ccttacggta tctatgattt aagctgggat tcccaataat accttgattt cagtacagac
     4081 cgaaaacccg aagagagtgc cttcttttcg ggttttctta tataatcctc gaatggcttc
     4141 catgccttta atcgtggtag aggcagtgcg taaacttcga tagaatttat tgcgtctctt
     4201 tactggacga tggtcttgtt caatcaaatt attcaggtat ttaatggtac gatgttctgt
     4261 cccttgataa aagccgtatt cttttagttt cttaaaggca cttgtaatag agggggcttt
     4321 atctgtgact acaaccttcg gttcatcaaa ctgcttcact aaccgcttaa gaaaagcata
     4381 ggctgcttgt gtgtcccgtt ttttacgtaa ccaaatatcc aaggttaaac catctgcatc
     4441 gatggctcga tacaaataat gccattttcc tttaattttg atgtacgttt catccatttt
     4501 ccatgaataa aaggattttt tatttttctt tttccaaatt tgatagagta gtttgccata
     4561 ttcttgcacc caacgataaa tcgtcgtatg agaaacgtta atgccacgat catataagat
     4621 ttcttgaact tcacgatagc taaggttata acgaagatag tagcccacgg ctacaataat
     4681 cacatcctgc tgaaattgct ttcctttaaa atgattcatc gtcattcctc ctgctatctt
     4741 tttctattat tctaccttat ttgatagtag atttaaaact ttgcaacaga accctataga
     4801 gaagaataaa ttcatttcta ctgacgtaaa tttgcgtaaa ggaatcgtat ttaattcatg
     4861 gtggtatttc acaatttcat ttgccataaa atcctccttc aagattaact acttaacaaa
     4921 attataaaga gctttctaat aggggtcaag cccctaatta aagaccccta aaaacagaca
     4981 aaaagacccc caaaaacaga caaaaagacc cctaaaaaca gacaaaaaga cccctaaaaa
     5041 cagacaaaaa gacccccaaa agattctgca caccttggga gagtaagcat acagccgacg
     5101 gtaaagaggt ttaaaaagag gtttaaaaag agattataaa agaggagagg ggcaaaattt
     5161 cccgcctaaa aattttctgc ttttttagcc aaacaggttc gcgaatttta ctaaactggg
     5221 aaacttgctc cctctggtcg ctaaaaaggt gtgagggaaa aacaccctca cattgtacct
     5281 gcgcagtcgc ctaaaggctc ctttgctaaa aacgttgttt aaagagattt ttttagaggc
     5341 tctgcgcgct cgtttaagcc gttttaagac agtttagtat ggataaccct tatcttgttc
     5401 ttaaagcacc ttacaggcgg tcagggcaag ccctaacaac ccttaaagag cataaaacca
     5461 tcctaaaatt cgatttaatc tgtcttatct atgttcttgt atattttata taccttgggt
     5521 agtaaaacgt ctgaaaacgc aaataagagc gtatgaaaca aaacaataag caaccgattc
     5581 agcagaattc cggaggagcc attttcgtac gttagcaaaa aagtgctgtt gctaaaagct
     5641 gcttgcggtg cggagcgaac aaggtctcct agagcgagac gtgtacaaac aagcttacaa
     5701 attcgtgaaa ggcaccggtt ttgcgccatt tccaagcaaa aaaaggtcta tcgagcaagc
     5761 tcgatagacc gatcgcattt agccccccgc attctaacag ggggggaata atgtgaaaaa
     5821 ttatggtagt aatgcttgat atataggcat ttcttttggt tgcacactta aaaaaaatca
     5881 cgattttgac aactaaattt ttaaataaaa aagctttaca gtttaattca aatgtgtttt
     5941 aatatatcta tcagtacttg ccacgcctct gatttaatat catgcgcatt atggtagggc
     6001 ttttttttta tattttttta agaaagaaga ttcttatgaa gacatcaaat agaaaatata
     6061 aagttaaaac agttaatcat agaattccac ctaaaaaggc attatgtgta attaataatt
     6121 ccaaagtaat taatcaagag ttaaaaccat atttatccga gcaagaaatg ataaaaaaaa
     6181 taagtaaaga tattttttta acaaattccg atacaatttt attagaaaag tttttaaaga
     6241 aaaatagtta ttttcgattt agtatttatg ttaaattaat gaaaaatata gataaagtta
     6301 ctattctaga tgttattgaa acgtacaaat tagattcatt cattcgtaat caattgcatt
     6361 attttgtgaa tcaaatagaa attttttgga aaaaatctct ttcagataat atgtgcgttt
     6421 cttatgaaga aactagcata tttccgaaga atcagtgtta tttggataaa aatatttatt
     6481 cagatttgaa atgggctgaa gatataattg gtcattttaa tagttttttt tattcaaatc
     6541 aaagtcccac ttttaaacat catcatataa aaaaaaatca ttgtttacca atatgggctt
     6601 tatttgaaga aataacattt ggtagtttaa caacatttat taatcaactt aatacagagt
     6661 attataataa ttgggtcatg agctgttatg ataatcctaa atacaaaaaa gctatacgaa
     6721 gttgggcaaa cgtaattaga gcttatagaa ataagatagc tcatcattca agaatatatg
     6781 catttaaagt tactgacgct ccaacaatca taaaaaatga ttccaaaagt tattttcctg
     6841 aaaataactt aaaagaaacc caaaagttat atttgtatgg aggattatac gttataaaac
     6901 atctgttaat atatgaagat agcatgacac aaactttatg gaataaattt ctaatagatt
     6961 tagaaaaaaa gatcgataaa atcccttcct tagaaaaatc caattatggt tttccggaaa
     7021 attggagaaa caagttaaaa ataatgattg tttaattgtt atttaaattg ttagtagaca
     7081 taatgtatat actaccaagt agcccctgga tcaaccgatc caggggaaga ctttgtgatt
     7141 ttcaattggt ctatctaaaa catcttttta aaagcttgat tcttcgctgt tggctttttt
     7201 cgattctttg tgaatattca gtgattattt ttttactact tgttattttc cgagggattt
     7261 gttaaaagtt taatagcgtt tctcagtaag ggacgtcgtt ttttcatttt cctatgtatg
     7321 gactgctcga acgagcagcc tttttttatt ccagggaacg tcctattctt tgagtttcca
     7381 tgctattgaa taaccgggac ttagggatta gggcagcgcc ccaaaatgaa aaaagaaaaa
     7441 tccgcttctg cgaattcttc ttttttttcg caagttggta ccaacttgca aatcttgctg
     7501 gaagaccgat ccctaaaaag aaatcgagga atttttttgt ttttcggttt ctttttttat
     7561 ctttttagaa agtttgttgg gtcaaaattt ccagaagtaa aggtttgatt ggtttctttt
     7621 tgacgatttt tgggttgatt tcgtcgggaa ggattgagtt gattttcaat tttttctttc
     7681 acttcttgat atactttaga atttgtaccg ataagaacac cagtatcttt tagatataaa
     7741 tcagcaaatt ctgccatgat ttctgtcatg atagaaattt cttttcccat ggcattgagt
     7801 tttgcttggt tactggttgt ggttgtattg gtatcaggtt cataactcat acataaaaag
     7861 cgaatggctt ctgaataatt gttaacgtct ggattttttt cgatgatgga atctaaaacg
     7921 tcaacgtgac tgggataaag ggttacgtgt ttttgaacag aagtttttct catgttatat
     7981 tcctccgata agtgtttcaa tgagttggtt gtttgtgtga acatagtcat ttagtatgtt
     8041 cgtattttct gcttgaatgg caataaattt ttcgaataat aaatcgaatt gatctttttt
     8101 gtaattcata agttcgtggt caaaatcgtt ttcgattaaa atttttaggt attgatttcg
     8161 actccatttt ttgcccgttt tggttgttag ttcctgacaa cgtttatcta ttcttgtaat
     8221 aattttggga ttgatatttc gaactaaaat gtccatggaa attttccttt cgtatcagtg
     8281 tataatttat gtatatcata caaatatagt atttgtatag ctatttttcg ccaaaacact
     8341 atacatagcc tagccacaat ggcaaaaaag tgatagcaaa tttttttctg agaaacgtga
     8401 taggcacagc ttatctagca cctttcgtaa gaaagtgata ccacgtttcc ttatgctctt
     8461 tcaaaattca gaaaaatcag ctgtaaaacg agtgcagtcc gagtaagatt caacgaaaaa
     8521 acggggattg gggcgccacc aaaatatcgc aacatgggcc catgttgctc tgctcgcaaa
     8581 agaaaaaaga agaataaaac cagcaatttt tattaattta aattgctggt tttattgcca
     8641 gaattgccac cattttttct ccgtctttga ttgtttttct atcgattctg tttcttcaga
     8701 aagagtcgtt gatttatttt ccgaagattc ttccgtataa gtcaactgta gttgttcctg
     8761 cagtttctcg atttgttggt tggcttgcag ggtgagttgt tgttgttggt cgagtaattt
     8821 ttgcatttgt tttatttgct cgtctttttc ttgtagttgc atatcttttt gatttaactg
     8881 ttgttctagg agagaaagga cttctaattc gggaacttgg ttggcgtttt tgtggttttc
     8941 ggttttagtt ttttcgccaa ctagttgttg atttgagttt tcgttttcgt taaaaaacat
     9001 tgattttata gccttttgtc cggtttcatt aatcacaaac ttgtttccga ttttcgaaaa
     9061 ccaaagtttt cgattttcgt cagaaacttt tttgttaatt gctgttttac taactcctaa
     9121 ttcttcagca agttctttga ttgttttttc tgtcataaaa tctaaaaacc cctcaatctt
     9181 tgaataagtt ttgaagattg tctaataagt ctttttttag ttctgaagga atatcggaat
     9241 ttatctctga tttttgtgca ttattttctt tattttgggt attttgttct ctttctaact
     9301 tttctaactg ttgtaaaagt tccaattttc gcttttgttc tttgattttt tcttgtaatg
     9361 aagtaggtgc tttttcagat tgaacttgtt ggttagctag ttctaatgct ttgttttgtt
     9421 cttcttttgt tggggcatgt ttgttaaagt cgtctgattt ttttatctca ggtttaaatg
     9481 taaattgata gccaatgaca ggctttcccc gtccttttcc gtatctcttt tggattgtca
     9541 gacctttgaa gattgcggtt agttcttggc gaataggctt tataattttt ttatttatat
     9601 cactttctct ataactttta gggatatcaa gtaattcatg aaagtcttct tttgaaaaat
     9661 aggcaattcc tttggttctg tattgtttta ataatctaaa catggttttg gaataactgc
     9721 tttctaattc attgaattgt tggagagaat atcgaaccca ttcttcaaga ttattaagta
     9781 agggtaaggc tttctcatgg acttgtattt cagcaaaagg aatatcagct tttccatcga
     9841 ttttaaattg attaaataaa acaaatcgtt cacgttgcaa accgtcagca cttcttctac
     9901 caaaacgtaa atccattaat ttgtcatacg ttgtttctaa atcatctatg aatcgaacat
     9961 ttgcagttgc tttataattg cttaaatctt ttaattgttc gaatggttct gttgcaaagt
    10021 tttaaataaa gaataaaatc ccttacggta tctatgattt aagctgggat tcccaataat
    10081 accttgattt cagtacagac cgaaaacccg aagagagtgc cttcttttcg ggttttctta
    10141 tataatcctc gaatggcttc catgccttta atcgtggtag aggcagtgcg taaacttcga
    10201 tagaatttat tgcgtctctt tactggacga tggtcttgtt caatcaaatt attcaggtat
    10261 ttaatggtac gatgttctgt cccttgataa aagccgtatt cttttagttt cttaaaggca
    10321 cttgtaatag agggggcttt atctgtgact acaaccttcg gttcatcaaa ctgcttcact
    10381 aaccgcttaa gaaaagcata ggctgcttgt gtgtcccgtt ttttacgtaa ccaaatatcc
    10441 aaggttaaac catctgcatc gatggctcga tacaaataat gccattttcc tttaattttg
    10501 atgtacgttt catccatttt ccatgaataa aaggattttt tatttttctt ttccaaattt
    10561 gatagagtag tttgccatat tcttgcaccc aacgataaat cgtcgtatga gaaacgttaa
    10621 tgccacgatc atataagatt tcttgaactt cacgatagct aaggttataa cgaagatagt
    10681 agcccacggc tacaataatc acatcctgct gaaattgctt tcctttaaaa tgattcatcg
    10741 tcattcctcc tgctatcttt ttctattatt ctaccttatt tgatagtaga tttaaaactt
    10801 tgcaacagaa ccaattatgc ggtaaagcta tataaagaag gaaatatgac tgtaaatcaa
    10861 atttgtgaaa ttactaatgt atctagggct tcattataca ggaaattatc agaagtgaat
    10921 aattagccat tctgtattcc gctaatgggc aatattttta aagaagaaaa ggaaactata
    10981 aaatattaac agcctcctag cgatgccgaa aagccctttg ataaaaaaag aatcatcatc
    11041 ttaagaaatt cttagtcatt tattatgtaa atgcttataa attcggccct ataatctgat
    11101 aaattattaa gggcaaactt atgtgaaagg gtgataacta tgagcgataa aatacttatt
    11161 gtggatgatg aacatgaaat tgccgatttg gttgaattat acttaaaaaa cgagaattat
    11221 acggttttca aatactatac cgccaaagaa gcattggaat gtatagacaa gtctgagatt
    11281 gaccttgcca tattggacat catgcttccc ggcacaagcg gccttactat ctgtcaaaaa
    11341 ataagggaca agcacaccta tccgattatc atgctgaccg ggaaagatac agaggtagat
    11401 aaaattacag ggttaacaat cggcgcggat gattatataa cgaagccctt tcgcccactg
    11461 gagttaattg ctcgggtaaa ggcccagttg cgccgataca aaaaattcag tggagtaaag
    11521 gagcagaacg aaaatgttat cgtccactcc ggccttgtca ttaatgttaa cacccatgag
    11581 tgttatctga acgagaagca gttatccctt actcccaccg agttttcaat actgcgaatc
    11641 ctctgtgaaa acaaggggaa tgtggttagc tccgagctgc tatttcatga gatatggggc
    11701 gacgaatatt tcagcaagag caacaacacc atcaccgtgc atatccggca tttgcgcgaa
    11761 aaaatgaacg acaccattga taatccgaaa tatataaaaa cggtatgggg ggttggttat
    11821 aaaattgaaa aataaaaaaa acgactattc caaactagaa cgaaaacttt acatgtatat
    11881 cgttgcaatt gttgtggtag caattgtatt cgtgttgtat attcgttcaa tgatccgagg
    11941 gaaacttggg gattggatct taagtatttt ggaaaacaaa tatgacttaa atcacctgga
    12001 cgcgatgaaa ttatatcaat attccatacg gaacaatata gatatcttta tttatgtggc
    12061 gattgtcatt agtattctta ttctatgtcg cgtcatgctt tcaaaattcg caaaatactt
    12121 tgacgagata aataccggca ttgatgtact tattcagaac gaagataaac aaattgagct
    12181 ttctgcggaa atggatgtta tggaacaaaa gctcaacaca ttaaaacgga ctctggaaaa
    12241 gcgagagcag gatgcaaagc tggccgaaca aagaaaaaat gacgttgtta tgtacttggc
    12301 gcacgatatt aaaacgcccc ttacatccat tatcggttat ttgagcctgc ttgacgaggc
    12361 tccagacatg ccggtagatc aaaaggcaaa gtatgtgcat atcacgttgg acaaagcgta
    12421 tcgactcgaa cagctaatcg acgagttttt tgagattaca cggtataacc tacaaacgat
    12481 aacgctaaca aaaacgcaca tagacctata ctatatgctg gtgcagatga ccgatgaatt
    12541 ttatcctcag ctttccgcac atggaaaaca ggcggttatt cacgcccccg aggatctgac
    12601 cgtgtccggc gaccctgata aactcgcgag agtctttaac aacattttga aaaacgccgc
    12661 tgcatacagt gaggataaca gcatcattga cattaccgcg ggcctctccg gggatgtggt
    12721 gtcaatcgaa ttcaagaaca ctggaagcat cccaaaagat aagctagctg ccatatttga
    12781 aaagttctat aggctggaca atgctcgttc ttccgatacg ggtggcgcgg gacttggatt
    12841 ggcgattgca aaagaaatta ttgttcagca tggagggcag atttacgcgg aaagcaatga
    12901 taactatacg acgtttaggg tagagcttcc agcgatgcca gacttggttg ataaaaggag
    12961 gtcctaagag atgtatataa ttttggctct ttgtcaaatg atgttggtaa agagaaatct
    13021 tagaataatt ggagatcaag tggatcagtt cctcttgatc ttttttagtt gaaaatcaat
    13081 gcgttgccat gacgaatcat gatgcgcaat ttgaaattat aaaagctacg aaagccatat
    13141 gcatttcgtt ttaacacttt gatatggttg ttcagacatt caagtgggcc atttgaataa
    13201 ctttgttgta aagcattttt gatttgtggc agaaatttct ggaacgttct caaggtcgtc
    13261 tgatagcctt cgggaagcat gatattattg gtaagtagtt cttcaaacga tacgtagtct
    13321 cgttccttta cagcgtaaag aaagtcttga tataactgat agcctacacg caaagcaggg
    13381 ctccctttca gtaagcgatc cacaatatcg gtctctgtta aatgagcttt gaagccaggt
    13441 cgccaaaggc gtcgagtata atcaagcttg cgttcatctt tttgaagaag tttccagtat
    13501 ctttttaatt gcttacctaa ttgatagtgt ttttgttctt ttgcttttta atagttggtt
    13561 cgtttctttg attcgatgat tgcgaaaggt acgaccaata tgttggacga tgtgaaaccg
    13621 gtcaataatc agctgtgctt ttggaaacag ctttttaacg agcgatacat aaggagcgta
    13681 catatcaatc acgatccatt cgaccgcttc acgaatttct cgtgaaaaat gactaaaata
    13741 acgttttaaa aaaggcagtt gtcggttctc gacaatgtcc aataagcgct gtgtttgtcc
    13801 atccatcatg atgaaactca tagcacctga aacaccccga actgatttga attcatcaaa
    13861 acaaagtacc ttaggtaacg gtgtccgaaa aggattcaac ggacgataaa atcgcttcat
    13921 aatacggtaa acagaagaga ccgatagatt tttctgagcg gcgatctctg tcattgaggt
    13981 attttttttc agacgagtca cgacagacca gcggactttc tcactgatga aacaatgttt
    14041 tttcgcaaca gaagtatctg cgacaaaggt ccgctgacaa gctttgcatt tgaagcgttg
    14101 ctttttcaat tcgaggtagg tacggtattc agaaacatcg ttcattaaca accggacggt
    14161 tgttataccc caacgcacaa tatctgaagc aatctggtgt ttacagtgga tgcattcttg
    14221 ggctgtataa atcaagtttc cacgataaat atgacagatt tgccctttgt atttttcttt
    14281 tgtgagacaa ttttcgttaa aagtaactga taagtctaga atatctaatg tttctttgat
    14341 aaggtgagtg taagacatct tgattcctcc ttttgggttg gttcgccaaa ttaattctaa
    14401 aggatttcaa ggtgtctttt ctatatgttt tggaaaataa aaaagatgct ggtgtcatcc
    14461 accaacatca aaaagtatag agccataatt ttttaggaaa atctcaaggt tatctttact
    14521 ttttcttagg aaattaacaa tttaatatta agaaacggct cgttcttaca cggtagactt
    14581 aataccgtaa gaacgagccg ttttcgttct tcagagaaag atttgacaag attaccattg
    14641 gcatccccgt tttatttggt gcctttcaca gaaagggttg gtcttaatta tgaataacat
    14701 cggcattact gtttatggat gtgagcagga tgaggcagat gcattccatg ctctttcgcc
    14761 tcgctttggc gttatggcaa cgataattaa cgccaacgtg tcggaatcca acgccaaatc
    14821 cgcgcctttc aatcaatgta tcagtgtggg acataaatca gagatttccg cctctattct
    14881 tcttgcgctg aagagagccg gtgtgaaata tatttctacc cgaagcatcg gctgcaatca
    14941 tatagataca actgctgcta agagaatggg catcactgtc gacaatgtgg cgtactcgcc
    15001 ggatagcgtt gccgattata ctatgatgct aattcttatg gcagtacgca acgtaaaatc
    15061 gattgtgcgc tctgtggaaa aacatgattt caggttggac agcgaccgtg gcaaggtact
    15121 cagcgacatg acagttggtg tggtgggaac gggccagata ggcaaagcgg ttattgagcg
    15181 gctgcgagga tttggatgta aagtgttggc ttatagtcgc agccgaagta tagaggtaaa
    15241 ctatgtaccg tttgatgagt tgctgcaaaa tagcgatatc gttacgcttc atgtgccgct
    15301 caatacggat acgcactata ttatcagcca cgaacaaata cagagaatga agcaaggagc
    15361 atttcttatc aatactgggc gcggtccact tgtagatacc tatgagttgg ttaaagcatt
    15421 agaaaacggg aaactgggcg gtgccgcatt ggatgtattg gaaggagagg aagagttttt
    15481 ctactctgat tgcacccaaa aaccaattga taatcaattt ttacttaaac ttcaaagaat
    15541 gcctaacgtg ataatcacac cgcatacggc ctattatacc gagcaagcgt tgcgtgatac
    15601 cgttgaaaaa accattaaaa actgtttgga ttttgaaagg agacaggagc atgaatagaa
    15661 taaaagttgc aatactgttt gggggttgct cagaggagca tgacgtatcg gtaaaatctg
    15721 caatagagat agccgctaac attaataaag aaaaatacga gccgttatac attggaatta
    15781 cgaaatctgg tgtatggaaa atgtgcgaaa aaccttgcgc ggaatgggaa aacgacaatt
    15841 gctattcagc tgtactctcg ccggataaaa aaatgcacgg attacttgtt aaaaagaacc
    15901 atgaatatga aatcaaccat gttgatgtag cattttcagc tttgcatggc aagtcaggtg
    15961 aagatggatc catacaaggt ctgtttgaat tgtccggtat cccttttgta ggctgcgata
    16021 ttcaaagctc agcaatttgt atggacaaat cgttgacata catcgttgcg aaaaatgctg
    16081 ggatagctac tcccgccttt tgggttatta ataaagatga taggccggtg gcagctacgt
    16141 ttacctatcc tgtttttgtt aagccggcgc gttcaggctc atccttcggt gtgaaaaaag
    16201 tcaatagcgc ggacgaattg gactacgcaa ttgaatcggc aagacaatat gacagcaaaa
    16261 tcttaattga gcaggctgtt tcgggctgtg aggtcggttg tgcggtattg ggaaacagtg
    16321 ccgcgttagc tgttggcgag gtggaccaaa tcaggctgca gtacggaatc tttcgtattc
    16381 atcaggaagt cgagccggaa aaaggctctg aaaacgcagt tataaccgtt cccgcagacc
    16441 tttcagcaga ggagcgagga cggatacagg aaacggcaaa aaaaatatat aaagcgctcg
    16501 gctgtagagg tctagcccgt gtggatatgt ttttacaaga taacggccgc attgtactga
    16561 acgaagtcaa tactctgccc ggtttcacgt catacagtcg ttatccccgt atgatggccg
    16621 ctgcaggtat tgcacttccc gaactgattg accgcttgat cgtattagcg ttaaaggggt
    16681 gataagcatg gaaataggat ttactttttt agatgaaata gtacacggtg ttcgttggga
    16741 cgctaaatat gccacttggg ataatttcac cggaaaaccg gttgacggtt atgaagtaaa
    16801 tcgcattgta gggacatacg agttggctga atcgcttttg aaggcaaaag aactggctgc
    16861 tacccaaggg tacggattgc ttctatggga cggttaccgt cctaatcgtg ctgtaaactg
    16921 ttttatgcaa tgggctgcac agccggaaaa taacctgaca aaggaaagtt attatcccaa
    16981 tattgaccga actgagatga tttcaaaagg atacgtggct tcaaaatcaa gccatagccg
    17041 cggcagtgcc attgatctta cgctttatcg attagacacg ggtgagcttg taccaatggg
    17101 gagccgattt gattttatgg atgaacgctc tcatcatgcg gcaaatggaa tatcatgcaa
    17161 tgaagcgcaa aatcgcagac gtttgcgctc catcatggaa aacagtgggt ttgaagcata
    17221 tagcctcgaa tggtggcact atgtattaag agacgaacca taccccaata gctattttga
    17281 tttccccgtt aaataaactt ttaaccgttg cacggacaaa ctatataagc taactctttc
    17341 ggcaggaaac ccgacgtatg taactggttc ttagggaatt tatatatagt agatagtatt
    17401 gaagatgtaa ggcagagcga tattgcggtc attatctgcg tgcgctgcgg caagatagcc
    17461 tgataataag actgatcgca tagaggggtg gtatttcaca ccgcccattg tcaacaggca
    17521 gttcagcctc gttaaattca gcatgggtat cacttatgaa aattcatcta cattggtgat
    17581 aatagtaaat ccagtagggc gaaataattg actgtaattt acggggcaaa acggcacaat
    17641 ctcaaacgag attgtgccgt ttaaggggaa gattctagaa atatttcata cttccaacta
    17701 tatagttaag gaggagactg aaaatgaaga agttgttttt tttattgtta ttgttattct
    17761 taatatactt aggttatgac tacgttaatg aagcactgtt ttctcaggaa aaagtcgaat
    17821 ttcaaaatta tgatcaaaat cccaaagaac atttagaaaa tagtgggact tctgaaaata
    17881 cccaagagaa aacaattaca gaagaacagg tttatcaagg aaatctgcta ttaatcaata
    17941 gtaaatatcc tgttcgccaa gaaagtgtga agtcagatat cgtgaattta tctaaacatg
    18001 acgaattaat aaatggatac gggttgcttg atagtaatat ttatatgtca aaagaaatag
    18061 cacaaaaatt ttcagagatg gtcaatgatg ctgtaaaggg tggcgttagt cattttatta
    18121 ttaatagtgg ctatcgagac tttgatgagc aaagtgtgct ttaccaagaa atgggggctg
    18181 agtatgcctt accagcaggt tatagtgagc ataattcagg tttatcacta gatgtaggat
    18241 caagcttgac gaaaatggaa cgagcccctg aaggaaagtg gatagaagaa aatgcttgga
    18301 aatacgggtt cattttacgt tatccagagg acaaaacaga gttaacagga attcaatatg
    18361 aaccatggca tattcgctat gttggtttac cacatagtgc gattatgaaa gaaaagaatt
    18421 tcgttctcga ggaatatatg gattacctaa aagaagaaaa aaccatttct gttagtgtaa
    18481 atggggaaaa atatgagatc ttttattatc ctgttactaa aaataccacc attcatgtgc
    18541 cgactaatct tcgttatgag atatcaggaa acaatataga cggtgtaatt gtgacagtgt
    18601 ttcccggatc aacacatact aattcaagga ggtaaggatg gcggaatgaa accaacgaaa
    18661 ttaatgaaca gcattattgt actagcactt ttggggtaac gttagctttt taatttaaaa
    18721 cccacgttaa ctaggacatt gctatactaa tgatacaact taaacaaaag aattagagga
    18781 aattatattg ggaaaaatat tatctagagg attgctagct ttatatttag tgacactaat
    18841 ctggttagtg ttattcaaat tacaatacaa tattttatca gtatttaatt atcatcaaag
    18901 aagtcttaac ttgactccat ttactgctac tgggaatttc agagagatga tagataatgt
    18961 tataatcttt attccatttg gcttgctttt gaatgtcaat tttaaagaaa tcggattttt
    19021 acctaagttt gcttttgtac tggttttaag tcttactttt gaaataattc aatttatctt
    19081 cgctattgga gcgacagaca taacagatgt aattacaaat actgttggag gctttcttgg
    19141 actgaaatta tatggtttaa gcaataagca tatgaatcaa aaaaaattag acagagttat
    19201 tatttttgta ggtatacttt tgctcgtatt attgctcgtt taccgtaccc atttaagaat
    19261 aaattacgtg taagatgtct aaatcaagca atctgatctt tcatacacat aaagatattg
    19321 aatgaattgg attagatgga aaacgggatg tggggaaact cgcccgtagg tgtgaagtga
    19381 ggggaaaacc ggtgataaag taaaaagctt acctaacact atagtaacaa agaaagccca
    19441 attatcaatt ttagtgctga ggaattggtc tctttaataa atttccttaa cgttgtaaat
    19501 ccgcattttc ctgacggtac ccccaaacta agatttcata gatagatttt ctgataactt
    19561 taaaaaacac gaatagcgga aaggatgtct tcattcgcta aagagtaata aactactttt
    19621 ccatcttgac gtgattttgc cattccatgt ttttttaaaa aacgtaaatg gtgtgacgtt
    19681 gtagcgacac tagcagtaag tatttctgct aaatcacaga cgcacatttc cttataggtt
    19741 tctaaagcat agaaaatttt aattctagaa aaatcgctga aacatttccc taacacaagt
    19801 aagtttgaaa aatctttttg atccaattta ttttttattc catttacttt ttctttatgt
    19861 acgacagtca cttggcaaac ttttttcatt catctcacct cacattcaaa cgattatttg
    19921 aatgaagtat acctatttga tcactctttt gtcaatctac cttaattcca atacactatt
    19981 ataagcaatt tcatgtattg aagtaaactt acttgcaagt gcgtttttga ttccaagtta
    20041 aaaacatatt attattctac ataatttcct aatcaaataa aactcctttg aaaattgttc
    20101 taaaggagtt ttattatttt gtatttctac taaaaatagc gtacaatgta attactaagt
    20161 aattacgaaa tgtaaaataa tggaggaaat aatatgatta caaaaacaag aaaacaagga
    20221 aattctatta tgttaacggt ccctaaagaa ttcgatgtgc ccaatggtgt tgaagtagaa
    20281 gctaagcttg tagagaatgg tatcctttat gagtttgtag agcctaaaaa agaatttttt
    20341 gattttagtg aagatatttt ggctgatatt ctttcagaag gttatagcaa acaagagatc
    20401 ttaaaagaat ttagaaatag aaaaaatgcg ttaacttctg catttaaatc tattgctgaa
    20461 gatactgttg caaattctaa accgatgaca aaagaggagt tggcagcaga aattggctta
    20521 tgagattcta ccttctaaac acgtaataaa gtatctaaaa aaattaaaag agaaaacatt
    20581 aaaagaacaa tttttaacga taatttatga tgagatagca gttcgtcctc attcgggaga
    20641 acaaaaaacg ggtgatttat caggaatttg ggctattgga ttcaagtatg caggtacgac
    20701 atatcgcgtt gcatatgaaa ttaaagataa tgcagtgata cctatcttac tatgtggaac
    20761 gcatgaaaat ttttatgaac aattaaaaaa gattaggtaa gaacccaatg tttcaacttg
    20821 agaggataga cgacacttta aaagataatt tttaacagct gagagttgta ataccctata
    20881 attttaaacc actaacctat atatttatga aaaatagaaa aaattaaaac gcattttaag
    20941 cctctttcaa attaaaagct tcatccttat gcacaaagag ggcttaaaat gcgttatttt
    21001 atattgtttt ttagtaataa taaaaaaatc actctaattc tcccataaat attatagatt
    21061 cctaaagatt aaaatagatt attccctatt gtcttttaag tagcggtaga gtgttgcctt
    21121 gctaatacca gtcatttccg taatttcttt tacgctataa acctgacttt tatacaattt
    21181 tactgctcga tcaacatctt tctggttcac tcgtggacgg ccacctttac gacctctcgc
    21241 acgtgcactt ttcaaacctt ctttggtacg ttcaacaatt aaatcacgtt cgaactgact
    21301 aaatgcttgg aagacagtca tcattaagcg tccgtgaggc gtgctggtat cgaagttttc
    21361 tttcaagctc accagcttaa catggtgttc ttcaaaatag gtaacaaggt caatcaaatc
    21421 ctttgtacta cgacccaatc gagaaaaact ttctacaatt accgtatctc ccggacgaat
    21481 tttatctttt aaacgattta gttggggacg aaccgctttg gttcccgtca ttttctcagt
    21541 taggatttca ttacaatttt gttcattgag taagtctaat tgacgattta gttcttgtga
    21601 acgtgtactc actcgggcat aaccataaat gtatccactc atgcttttct tcctttcgta
    21661 ctttaattct ggcttgattg tatcataaaa gggagttata gatacatagt taatgcaaca
    21721 agttatgata caaaatatga cctcttttat ttaataaaag aggtcatttc attttgtctc
    21781 ataatcgatt gtttttgata cgagtgtgta tacgaaataa acaaaaaaat cttaagtaaa
    21841 tttctagata cctaatttaa tcctaactag tgtatcctaa agttaataac gagtttatca
    21901 caatgaaaat tataaaatgc tcccatatta gatataacat gcaatatttg aattagaact
    21961 acaaaattta ttcgttttat aaagtaaata atgttgagat actaaggaat agaatattat
    22021 aaggaattga gctgaatgta ttggataaaa aaagaataac tttattagag atggactata
    22081 aaaatgctcg taattttctt ctgaagtctg aaagctattt gaatgctcag ctaccagaat
    22141 atattagttt tgacgaaact ttaaaatctg ctaaaaaagt tttaacttcg ccgtttgtat
    22201 aattaagcga gacaaataaa aaagccattt atgttacact caaattgtaa tacccgctaa
    22261 agcatacaag gagagtgaac ataaatgact tatacccatc ttacaccaaa cgagcttgta
    22321 atgatagaag catattttca tcaagaaact ccggttgcta tcgttgcgaa gcagcttaaa
    22381 cgtggacgcc aaacaattta caatgtctat aactttctca aatgtggtgg aacagcactt
    22441 gaatactttg aacaatacaa agaaaataag cgacgttgtg ggagaaccga aatcattttt
    22501 cctgctgagg aaaaagaata cattgaaaaa agatcaactg aaggttggac cccagacgtc
    22561 attattggcc gtgcagagcg aaccttctct tgttcaataa gtaccctcta tcgccggttt
    22621 aagacgggag aattcaatgt tttacattta ccgatgcaag gaaaacgaaa accaaatggt
    22681 tataaggaaa aacgtggaaa acaggcattc aaaagaaata tttctgaacg taaaaaagat
    22741 tatgtcgttt tcgaagaaga atttggacat ttagagggtg atacgattgt cggcatccac
    22801 cataaaagtg ccgtcatcac actcgttgag cgactttcaa aagccattat cgtcttgaaa
    22861 ccagaaggcc gtaaggcagt tgatattgaa aattcgatta atgaatggct tcaatccgta
    22921 cccaaaaatc ttttcaaatc aattaccttt gattgtggaa aagaattctc taattggaaa
    22981 agtattagta atacgaatga tattgatatt tatttcgcag acccaggaac gccttcccaa
    23041 cggggattaa atgaacattc aaacggactc ctccgaaaag atggactacc aaaagaaatg
    23101 gacttcaacc aagtcaatca aggattcatc tcatccgttg cgtccaaaag aaaccatatc
    23161 cctagaaaat cactaaatta ccaaacacca ttagaagttt ttttgagtta cgtaaatgga
    23221 aagttttgtc tcgcttaatt tgacaaataa tacttcaaaa aaaaaccaac cgaaagatat
    23281 aaaagatatt gtagatacac gaatgtataa agaacgtgat gacttaaact atacgcttat
    23341 tatcaataaa gatggaaact attcatggcg tcctctcgtt ctactacatc ctattctata
    23401 tgtagatttg gttaattgtc tcactaaaga agaccattgg gaggagttaa aaaagaggtt
    23461 tagtgaattt aaaaaagata aaaatattaa ctgttattct atacctgttg aagcttccaa
    23521 aggttcaaaa aggacagatc ttggtgaaac aatactaaac tggtgggaat attttgaaca
    23581 aaaaagctta gctcagaata ttaaatacca atattgcatg tttactgaca tcgctgattg
    23641 ttatccatca atttatactc actctataac atgggccatg tatggtaaag aaaaagttaa
    23701 aaatgatgaa aataagtgtt cgggctcact tggcgataaa atagataaaa aaatatctgg
    23761 aatgcaacac aatcaaacaa atggtatccc tcagggttcc gttcttatgg attttgtagc
    23821 agagatcgtt ctagggtatg ttgatttgag attatcgaaa gaattaaaaa aattagaaaa
    23881 tttaaaagat tacaaagtac tcagatatag agatgattat agaatatttt caaataatcg
    23941 taatgatttg gaacaaatta tgaaagtgtt atcagaagtc ttatatgatt taaattttaa
    24001 gttaaattct agtaaaacaa aattatcaga tgatattatc atagattcta tcaaaaaaga
    24061 taagctctat tgggaagaaa ttaaggcaag tataagaatg aatgccacaa acttagatgg
    24121 tgatagagaa attcgttata gtataaattt acaaaaacat ttattggaaa taaaaaagtt
    24181 agcagataag catcctaatt caggccaatt gaaaaaagca ctttctgagt ttaataaaga
    24241 acgcattatt aaaatcactt caaagccgaa cgatctttta ccacttattg gtatcctggt
    24301 agatatcatg ataaataacc cgggttcttt gccgcagtgt gtcttaatta tagggcagtt
    24361 attaaatttc gaaaatgaga gtaaggctag agaaatagtt gaaatgatta taaacaaata
    24421 tgctttaaag ccaaatactg aatttatgat gatatggttt caaagattga ccaccatttt
    24481 tggagaaatg tttgatagtg ttttatcatc cgaatttgta tctaaaatca aagatcctgt
    24541 ctctaatcag atgttttctg tagactggct aaatgaagat gctaagaaat ctttttatga
    24601 agggaccatt aaagacgaag agaaatatat taaaatgagt aagaaaatat atattactga
    24661 atttgatgat tttaatgatt atccttttta agtgaaaatt tagaatgaat ataaaaaaca
    24721 ttacaatcta atttttttaa aaaaatgtta tatcggaagt aatctcttga tttctatact
    24781 ataagataag aaaaccacta tttaacaagg ttattcgaac cttattttaa gttacttatt
    24841 atttaatgta tcgaaaaagg tgaaataggt ttgttataat aagaattaag taagacatga
    24901 attgtctaag caaatgtagc ttatccattt cattaaaata catatggtga aaaagattac
    24961 gtccatataa ttgtgtaaaa gtgaagcatc aaaaaagcca ttgatctcag ttaggagtac
    25021 aatgtttttt gcagaaaaca tactcgaata ggactgatat caatgactca agtacatttt
    25081 acttttgaaa gcgaagaaat tcaagcaatt ataaacgaaa gcggtgcgaa tgataccgca
    25141 aaaacactta tgactatcat gtttaatcag ctgatggaag aacaacgaaa tcaatatatt
    25201 caggcaaatg cctatgaacg ttcagaagaa cggcaatcac aaagaaatgg ttactacgat
    25261 cgttcgttta caactcgaat tggtacgtta gaacttcatg ttccacgaac cagagacggt
    25321 aagtttagtc ctactatctt tgaaagatat cagcgaagcg aaaaagcttt gatcgctgcc
    25381 atgattgaaa tggtcatttc tggtgtctcc actcgcaaag taactaaaac agttgaactg
    25441 ctaacggatg gtgcgactgt ttctaaatca ttcgtttcaa atctgatgaa acagttggat
    25501 ccatttgttt ttgagtggag aaaccggagc ctagaaggct cggaatatcc atttttcatg
    25561 tgtgatgccc tgtatatgaa agtaagggaa aatcatcgta ttgtctcaaa aggtgtatat
    25621 attggtatcg gcattgattc tgatggacga cgtacgattc ttggttttga tgttcaagac
    25681 ggcgaatcgg aagataattg ggataccgtt tttcaatcgt tcgttcaacg aggtttgttc
    25741 ggtgtaaaac tggtcatttc tgatgcacac aaagggttag ttaaggctgt tcgcaagaac
    25801 tttttaggtg cgagctggca aagatgccaa gcccactttt tgagaaatat ttttgataag
    25861 ctgccaaaga aagtttcttc tgatgtaaaa gacgagttga agagtatctt taaagcctct
    25921 gaacttgaat tgacacgtga aagaaaagag cacttcttgg aaaaatatgg ttgtgattca
    25981 aaattaagtg ctgcttgcga cattttagaa aacggctttg aagatgctat tcaaattttg
    26041 tcttttcctg aaaatatccg ccgaaggatt cgtacaacca atgtgttgga acggttgaac
    26101 gaagagattc gccggagaga acgagtgatt cggatttttc caaatatcaa ttccatcact
    26161 cggataattg gaacactttt gatggaaaaa gacactgagt ggctggcttc tccacgaaaa
    26221 tatttagaat ttaattcgaa taacatttag caccatgaac ctgtgcgagt ctcttctttc
    26281 tgaggagaga catcaagagt cgctgcgcta ctgctcctga cctctctcct cagaaagaaa
    26341 ggcatggcag ttaggttcac aacgcaaaac attgtgcttt tgatgaggtt gaacttttac
    26401 acaagattgt ggacttgact gtgaaaaaac aagttcaaat agtaactaat tttttaaaaa
    26461 actgatttat aaaaaataca acacgaatga ggaaaataac caatgacaga acaaatcgaa
    26521 aaaaattcaa aaagcttata tcaggcactg tggaattccg cagatatcct gcgttcaaaa
    26581 atggacgcta atgaatgcaa gagctattta ctaggcttgg tcttttataa atacttatcc
    26641 gacaacatgc tgcggtatgt ctccgttcta ttggaggaag aaacggacga cctaaataaa
    26701 gcacaagacc tttacgttga agcatttaaa gatgcagata taaaggatga tctcttgtat
    26761 gaactaaaag atgagttttc atataccatc gcacccgcat tgacctttac cgcgcaagtg
    26821 gcagccattc atgatggatc ttttcaatta gaagacctgg tacaaggttt ccgtgacatt
    26881 gaacaatcca gcgaaaactt tgagaactta tttgaagaca ttgatctgta ttctaaaaaa
    26941 ctggggtcaa ccccacaaaa acaaaataaa accatcgcag atgttatgaa agaattagaa
    27001 gggttaaaca tggcggggca tgctggcgat gtcttaggcg atgcctatga gtacttaatt
    27061 ggccaattcg catctgaatc cggtaaaaaa gccggtgaat tctatacccc acaaccggtc
    27121 gcaaaattga tgacacaaat tgttttacaa ggaaaagaag ataaaaaagg cttttctgtt
    27181 tacgacgcga ccatgggttc gggctcgtta cttttgaacg ctaaaaaata ttcccatcaa
    27241 ccgggaacca tttcttattt cggacaagag ttgaatacgt ctacctataa tttggcgcgt
    27301 atgaacatga ttttgcatgg ggttcccatt gccaaccaac acttgcataa tgcggatacc
    27361 ttggatcaag actggccaac agaagagcca acgaacttcg atggggtgct catgaaccca
    27421 ccgtattctg caaaatggtc ggctgataaa ggcttcttag atgacccacg cttctctgcc
    27481 tatggcgtat tagcccctaa atcaaaagcg gactttgctt tcttattaca tggctactat
    27541 catttgaaag atacgggcgt catggccatt gttttgccac atggggtgct ctttagaggg
    27601 ggcgcagaag ggaagatacg caaagccttg ttagaaaatg gtgccattga caccgtcatt
    27661 ggcttacctg ctaacatctt ctttaatacg tccattccaa cgaccgtaat tatcttgaag
    27721 aaagaccgtg cgaaaaaaga tgtgctcttt attgatgcgt cacaagattt tgaaaaaata
    27781 aaaacgcaaa atacgctacg agattaccat attgacgcta ttttagaggc ctataaaacc
    27841 cgtacaatta aagacaagta tgcttatgtc gcagaatttg atgaaattgt ggagaatgat
    27901 tacaacttaa acattccccg ttacgtggat acctttgaag aagaagaagg gttctgttgc
    27961 aaagttttaa ataaagaata aaatccctta cggtatctat gatttaagct gggattccca
    28021 ataatacctt gatttcagta cagaccgaaa acccgaagag agtgccttct tttcgggttt
    28081 tcttatataa tcctcgaatg gcttccatgc ctttaatcgt ggtagaggca gtgcgtaaac
    28141 ttcgatagaa tttattgcgt ctctttactg gacgatggtc ttgttcaatc aaattattca
    28201 ggtatttaat ggtacgatgt tctgtccctt gataaaagcc gtattctttt agtttcttaa
    28261 aggcacttgt aatagagggg gctttatctg tgactacaac cttcggttca tcaaactgct
    28321 tcactaaccg cttaagaaaa gcataggctg cttgtgtgtc ccgtttttta cgtaaccaaa
    28381 tatccaaggt taaaccatct gcaccgatgg ctcgatacaa ataatgccat tttcctttaa
    28441 ttttgatgta cgtttcatcc attttccatg aataaaagga ttttttattt ttcttttcca
    28501 aatttgatag agtagtttgc catattcttg cacccaacga taaatcgtcg tatgagaaac
    28561 gttaatgcca cgatcatata agatttcttg aacttcacga tagctaaggt tataacgaag
    28621 atagtagccc acggctacaa taatcacatc ctgctgaaat tgctttcctt taaaatgatt
    28681 catcgtcatt cctcctgcta tctttttcta ttattctacc ttatttgata gtagatttaa
    28741 aactttgcaa cagaacccga agaacaattg tatttggaaa gtgaaacgag tcaaacgaag
    28801 gtcacagtca gccataatcg tttagacgtg tataaacgtc gtcctgaaac ggtaaaaaaa
    28861 ggaacggagc ttctacgact agaaacagaa aaatacatca tgaatgttta cctagaaagg
    28921 agtgagctgg tcgaaaactt tgtcagctac acgatttgca ggaaacacta ttactcagaa
    28981 tcagaatatt attcaatttt tgagagcatg gaattaatgg aagaagaaat ggaagagcta
    29041 aacagcaaag atgcgacgat agacgattat taccttgtat ttactagatt tttgacgtgt
    29101 ggaacggaat ctatcgtgtt aatgggaatg gattatgaac tcgctgtacc cgaagcattt
    29161 ttgagggaat atgtagaaga agtagataac ccaggcgacg actggtacac gactttaact
    29221 gattttttag acacctatac ttacgacgat acagaagaaa tccaaaatga acttgaccgt
    29281 aaacgaatgc catatcaacg atttgaaagg aaaaaaaggc aataatcatt tataccgaaa
    29341 agtgagaatg tgtgaaataa ggacaaaaaa cctagtaact aagggaatgg ttcttatttc
    29401 gcaccaatcg ctccaaccgc ccccgccgct taggtggtgc gaaataagcg ggcaacaccc
    29461 gataacctaa gagaaagaag gagatccata tgaataaaat cattcctcta ttgttagtcc
    29521 tatttgttgg cgaaatactt atgcgccatg ctgcagctgt gcatttaaac ttcgtagtat
    29581 ttttggcaat actggtattt tgtttgatct tttggcgctt tgtattagcc cttgtgagtc
    29641 tactattcaa gatgattgca gccatatttt tcatagggtt atttcgatga aaggagagag
    29701 tactaatgcc aattgcaata ggaaataaac ggttacccgt tacgttggat gaaaagagac
    29761 aaaaagaatt gcagcaacta aagcagaagt acggcaaaag tgaatccagg attatgtgta
    29821 ttgcgttaga tttattgatt gcccaagaaa aagcaggatt tgaggtacca gcactcaaaa
    29881 agtgacgtca ctttttatcc taaaaactaa aagtgatagc acttttaatt ataagaagtt
    29941 agaatattaa tcatttgctt aattgtacaa tataatgtac aattgtttta tagaaataaa
    30001 taaggggtga aaggaatgga agcagtagct tattcaaatt tccgccaaaa tttacgtagt
    30061 tatatgaaac aagttaatga ggatgctgaa acacttattg taacaagtaa agatgtagaa
    30121 gatacagttg ttgtattatc aaaaagagat tatgattcta tgcaagaaac gttgagaaca
    30181 ctttctaata attacgtcat ggaaaaaatt cgtcgaggag atgaacaatt ctccaaaggt
    30241 gcatttaaaa cacatgactt aatcgaggtt gaatctgatg attaaggctt ggtctgatga
    30301 tgcttgggat gattatcttt attggcatga gcaaggaaac aaaagcaata taaaaaagat
    30361 taacaagtta ataaaagata tcgatcgttc cccctttgct ggattaggaa aacctgagcc
    30421 attaaagcat gatttatctg gaaaatggtc cagaagaatt acagatgaac atagactgat
    30481 atatagagtt gaaaatgaaa cgatatttat ttattctgca aaagatcact attaaccaat
    30541 cggaagtaag gaaagggtca gaaacttaaa agtttttgat ccttatttta tttaccctag
    30601 tcatttaaaa agctaatata gcttagtgtt gattgttatt aatgaatgtg tttgttacgc
    30661 gtattacgga tataaggtta gtaaaatcat ttctaaagtt gaggaaaagt aaatataaat
    30721 ggcttaaatt tcaacaattt gaagttgaat agatatgtta taatactatt gtagtgtggg
    30781 atgttagtta ctaaaggatg acgcttatat atatgactga atagaataag caataggttt
    30841 aataatctat tttaaatttt ttgtactagt tttagttaat tagcaaaaac aacaaaaata
    30901 aacttctcat agaatttagc taaaaattaa tgatttattt acatattaaa tttggataca
    30961 gttaagtaat ttttatatat tggaggagaa gtaatggaat ataaatttaa cttgaatttg
    31021 aaagaagtac tccccactca gggaacaata aacataaatg gtcaagataa tgatgacgat
    31081 atagtaataa aaaaaataga atttattgat tctgatataa atgttttttt ttattctaaa
    31141 gaaaaattag ctgattttag caatattgct gaaatgattg aacgtttaaa agattttatg
    31201 atcagtgttt gggatgtagc gatgcttgct gacgaatcaa attatacttc gagcatatac
    31261 agattagaag atagtaattg gatagaaatt tcattagagt taaagacact tcctaattcc
    31321 gttaactata taattagaga tataacttct gaagctcaac aattagttaa ttacatgcat
    31381 ggacacgaaa gtatcccctt ttctcttata ctccttaaaa gaagtaaaaa tatatcaaat
    31441 actagagata ggtttatttc gataattaca gcctgtgaaa taggggtaaa ggaattttat
    31501 caacaagaga aacctgattt aactctaata tttgagaatt tacaatctcc ccctgtagac
    31561 aaattattag ggagcatgtt taaagggtac tttaatattg agtttccgaa agaaataaga
    31621 aaggaaatta aaaatttcat agaaaaacgt aataaattgg ttcattctcc tcaaggaagt
    31681 aaaccaacta tagaagaatg ttttgaatgt tatatggcag tgctaaaaac atttaatttt
    31741 ttttataaaa ttaatgatag ctttatgtat agagattttt atgaagaatc tatagaattg
    31801 actccaacgg aaccaaataa atctcaaatc actttgtcaa aggagctaga gagaaaagta
    31861 gagtctcaag aactagctat aggaattagt ataaccaatg tgtcgacata caaagaatta
    31921 tacgaagcta aatcacaaga taataaggat tttgtagaaa atatagataa ggaaccaaca
    31981 aatacagtag gggattaacg taaataagtg aagattatat taacagactc tttatttagt
    32041 taagtaaaaa atggtttgtt ggaattttat gctttatctg ttctgagtaa gacttaattt
    32101 tatagatagt aattatatgt aggaaattaa agatgagaag ataataaaag cagcatgatc
    32161 aactatttgt cattgaccat gctgtctttt tttattctat acacggtttg tctcgtaaca
    32221 ttgtagtctt tagcaatctt agcaatcgca acaccatttt taagatcttc aacgatgctt
    32281 ttgtaaacaa ggcgacgttg gggatctttt gcgtctgcac tgtataattt gggccgccct
    32341 ttgtagacgc cgtttgcttt tgcgatctta attccttgtg cctggcgacg tttcgattca
    32401 gtccgttctt gttcagcgat catggccaaa atttggatga tcagatcctt aatgaattta
    32461 tccaggagag gattgccaat tgcttcagcc atgatcggta aactagtaat gatcagcttc
    32521 acatttttct tcttcaagta attgactgaa gcgatgatct cgtcataatt tcttccaagt
    32581 cgatcaatag cttccacaat aaaaatatct tgatctcgga cgtaatctaa tgcttcttgg
    32641 aaaacaggtc gatgagtaat cgttgcgcca gattgtttct cgatgaaaat tctctctgca
    32701 ccgaattttt tcatttcttc aatctggcgt tgttcatttt gatcgatcga tgagacacgt
    32761 atgtatgcaa ctctcaaaac ttttacctac tttctgcaac tatttaaaat acaccctaat
    32821 catacacgag gaagccatat ttttcaatgt tataccagtg tctatttagg gtaaacctta
    32881 tatagacaaa aactaatgat ggaggaaata gtatgagaat tttttgtaag tgtctactga
    32941 taacttattc tcaaatgttg taaggatttt attcatttgt taatattaaa gaacggaact
    33001 ccaaaaaagt ccgtactcta atttgcagtc tctcactctt ttagagtgag agtttttatt
    33061 agattttcaa attaattagt aaagtgatag aataaattga tcatacaaat caaaagaaga
    33121 agcagggtga tagcatacct atgcttcttt ttttatgtat ttagctattt cttctttaca
    33181 atatcgaaca tatgtttgta taatgtgaaa gaatggagtg ttatttatga tggattattc
    33241 aaaagagcca gtaaatgatt actttttaat cgatatgaaa agcttttacg caagtgtcga
    33301 atgtatagaa agaaatttag atccattaac aacagaactt gttgttatga gtcgagctga
    33361 taatactggt tcaggattga tattagcttc ttctcctgaa gcaaaaaagc ggtatggtat
    33421 tacaaatgtg agtagaccac gtgacttacc acaaccattt cctaaaacac tacacgttgt
    33481 tccaccacgt atgaacctat atatcaagcg aaatatgcag gtaaataata ttttcagaag
    33541 atatgtggct gatgaagatc tactgattta ctcgatcgat gaatcaatct taaaagtgac
    33601 ccgatcactg aatcttttta cgactgaagg aacacgaagt caacggagaa agaagctggc
    33661 tcaaatgatc caggagaata ttaaagaaga gctaggattg attgctacag taggtgtcgg
    33721 agataatccc cttttggcta aactagcctt agacaacgaa gcaaagcata atgaaggatt
    33781 tgttgcggaa tggacgtacg aaaacgttcc tgaaaaagta tggaatattc ctgaaatgac
    33841 ggacttttgg ggaattggct ctcgaatgaa aaaaagactc aatcagatgg ggattatgag
    33901 cattagagat cttgctaact ggaatcctta tacgattaag aatagactag gtgtgattgg
    33961 cttacagctg tacttccatg ctaatgggat cgatcgaaca gatatagcaa tcccaccaga
    34021 accgacaaag gaaaaatcat atggaaattc acaagtgtta ccacgagatt atactagaag
    34081 aaatgaaatc gagctggtgg taaaagaaat ggcggaacaa gtagcgatac gcattaggca
    34141 gcataactgt aaaacaggtt gtgtccattt acacattggc acgtcgatct tagaaacgag
    34201 accagggttt tcacaccaga tgaagatccc tatcacagat aatacaaagg aattgcagaa
    34261 ttattgtctt ttcttgtttg ataagtatta tgaaggacaa gaagtaaggc atgttggaat
    34321 aacctatagc aagcttgtgt acaccgattc gcttcagctg gatttattta gtgatccaca
    34381 aaagcaaatt gacgaagaaa atttagataa aattattgac aagattcggc aaaaatatgg
    34441 ttttacgtcg attgtacatg cttcttcaat gttagaaggc gctagaagca ttactagaag
    34501 cacacttgtt ggtggtcatg cgggcggaaa tgggggaatt aaaaatgatt gattacaatg
    34561 atcgtggacg tttaaagtgg gcagggtttt atttatctga tcatactgaa gaaattgatg
    34621 ccgacaatga acaacgaacc agtcaaaatt tagcaaaaga acaaatgacg actgaagaga
    34681 ttgcggaagt attgaacgct gcagtcataa aaaatagatc agtatctatt caaaaagaag
    34741 aacgaaacgc tgaaggatac tatccagaag atatcattgg caagatctta ggtagtgatg
    34801 agctggggat ctacgttgga aaagacaaaa ttcactacga cgaaatacgt catatcgaat
    34861 tttaccagga taataaatgg agtcgattag aatgaacatt atccagcaat atgagctaaa
    34921 gtatataacc tttgatcagc tatcagaaga gatttggggt tatggccaac gtttgataaa
    34981 tgaggttggt gttgagcgct tctctttcta tgtagaagct gcagcaggtt atcataattt
    35041 taggttttat atcttcccgt tatatatata aggtatttta cggattctat agagttatct
    35101 ctatgaatcc ttttttgttt ttacaaatag gagtagatac tagaataatg cgtgtgttaa
    35161 actattattg caaacgcaat aataaaagaa agtaggctta cgcaccatgt ctatcaatac
    35221 atttgtttac tcgcacccga tcaatgttta cattatcaag aatttaggaa tcactgtaga
    35281 acaattttgt gagttatatg cctacccaca aggaacggtt gcaagctgga taacccgaca
    35341 acgtagaata aaatcattac ctgcttcatt cgtttatgat ttaagcctgg cctctagttt
    35401 aaacatgtct gatgtttatg aaaagctcct gtctttagaa aaagaatatg attccttcaa
    35461 gattaaacat gataaaaaaa taaaaaaaca tatataatat caatcacaat tgtataatat
    35521 tacgatatct taatatcatg atatcgtaat attgtttttt tctaagaaaa tgcactataa
    35581 gaagggaatt attagtaaca atatagacag tttagtaatg tttaaattat tctagttgct
    35641 gctcgcaaaa catatttctt atcattaata aaaaatttta aagtaataaa aagtgattat
    35701 tttaatttcg aataacatat gaataatctt tatatccgaa ccgatatata tctttaaaac
    35761 aaaataatca cacaatatta caatataatg catatacgat attgcaatat cacaatatta
    35821 tgatataatt atattataat ataagatgaa tgggggatat tttaatggca agaatattgt
    35881 cagtagccaa tcaaaaaggt ggagtttcta aaactacgac tagcgttaat ttagcagcat
    35941 gtttgaaatt tgtttacggt aaaaatgtac ttttagtgga cattgatcct cagggaaatg
    36001 ctacagataa tgtaggtttc gatatagatg gaacaaacca acctacaatt tatgaagtat
    36061 taaaagggga ggctgacatt acagatgcta ttctggacta taaaggaatt gatgttttgc
    36121 cagccgacat agccctttct tctgcagaga gggaatttac tcaagtagga agtgaacaca
    36181 ggcttaaaag agtacttcaa ccaatcaagg acaactatga ttatattata attgattgtc
    36241 caccaagttt aggaatgctt acagtcaacg catttactgt ttctgatgaa attatcattc
    36301 cagttgaagc tgcgtatttc tcattaaaag gactaataaa gttaagtgaa actattgaaa
    36361 cggtaaaaga atatacgaac gacaagcttg aagtacgagg agttctattt actaagtata
    36421 ataagcgtta taacatttct aaagaaatga cgtctgcagc aaccgaaatt tctagagtca
    36481 ttggtgcaaa aattttcaaa acttatattc gtaggacaat aaccgttgac gaagcgcaag
    36541 ctgctggatc agatctaatc aattttaata aaccctcaac agctgaagat gactataaag
    36601 catttacaga agaatattta aaagaagttg gtgattttga tggcagaaga taatcgcagt
    36661 caagctgcag taagtagatt actaggaaat aaaaataaaa agaattttaa atcaaaagaa
    36721 gtagaagaaa tcgaattaac caagaaaaat ataagtaaag tcataaaaga tcaaggaagt
    36781 agtgtaagaa cagcagatag cattcgtata aatccaattc taatgtcagc actcaaatat
    36841 tggacgacta ttgctgaatc agacaaaagt aaaccagacg ttatagaaga tgcgctatta
    36901 aagtccatac ctgaagaata cttgattgaa ggatataaac tagcaaagaa acaaaataaa
    36961 atttaattta cattcaatat cacgatatca taatatcgtg atattttttt tataaaaatc
    37021 agtagtatca aagctgataa gtcgcttatc ttcatattgt aatattacga tatcgtgata
    37081 taatattatt gtaatatcgt gatatagtta ctatgagctt aaaccttgaa atagagcggt
    37141 ttgctttaat ttatttttta aggtaaacta aaaaaagata aaaaaagact actgctttaa
    37201 caaagtttgg cgacttatca gcagtagaaa aatatatagt ggatagatat atttgtttaa
    37261 ttcattgtaa catagttttg aacaaaacgc tatcctacag aaagaggatt ggct
//
