LOCUS       NZ_CP035481             8166 bp    DNA     circular CON 18-JUN-2024
DEFINITION  Escherichia coli strain U13A plasmid pU13A_D, complete sequence.
ACCESSION   NZ_CP035481
VERSION     NZ_CP035481.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN10817700
            Assembly: GCF_004135165.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8166)
  AUTHORS   Forde,B.M., Roberts,L.W., Phan,M.-D., Peters,K.M., Fleming,B.A.,
            Russell,C.W., Lenherr,S.M., Myers,J.B., Barker,A.P., Fisher,M.A.,
            Chong,T.-M., Yin,W.-F., Chan,K.-G., Schembri,M.A., Mulvey,M.A. and
            Beatson,S.A.
  TITLE     Personalised genomic investigation reveals strain heterogeneity
            during chronic infection with E. coli ST131
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8166)
  AUTHORS   Forde,B.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2019) School of Chemistry and Molecular
            Biosciences, The University of Queensland, Building 76, Cooper Road,
            St Lucia, QLD 4072, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2017
            Assembly Method                   :: HGAP v. 2.3.0
            Assembly Name                     :: UTAH_U13A_1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 120.0x
            Sequencing Technology             :: PacBio RSII; Illumina NextSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_004135165.1-RS_2024_06_18
            Annotation Date                   :: 06/18/2024 04:39:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,163
            Genes (coding)                    :: 4,925
            CDSs (with protein)               :: 4,925
            Genes (RNA)                       :: 113
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 91 of 238
            Pseudo Genes (incomplete)         :: 176 of 238
            Pseudo Genes (internal stop)      :: 41 of 238
            Pseudo Genes (multiple problems)  :: 60 of 238
            Pseudo Genes (short protein)      :: 1 of 238
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP035481.1.
            Bacteria and source DNA available from Schembri lab, The University
            of Queensland, QLD 4072, Australia.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8166
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="U13A"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pU13A_D"
                     /geo_loc_name="USA:Utah"
                     /collection_date="2013"
     gene            complement(259..962)
                     /locus_tag="U13A_RS26780"
                     /old_locus_tag="U13A_D00001"
                     /pseudo=""
     CDS             complement(259..962)
                     /locus_tag="U13A_RS26780"
                     /old_locus_tag="U13A_D00001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            complement(1495..1749)
                     /gene="mobC"
                     /locus_tag="U13A_RS26790"
                     /old_locus_tag="U13A_D00003"
     CDS             complement(1495..1749)
                     /gene="mobC"
                     /locus_tag="U13A_RS26790"
                     /old_locus_tag="U13A_D00003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091745.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVIVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPGQ"
                     /name="mobC"
     gene            1928..4087
                     /gene="mobQ"
                     /locus_tag="U13A_RS26795"
                     /old_locus_tag="U13A_D00004"
     CDS             1928..4087
                     /gene="mobQ"
                     /locus_tag="U13A_RS26795"
                     /old_locus_tag="U13A_D00004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_001029343.1"
                     /translation="MAIYHLSMKIISRNSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPRELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIVLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGASGLAALRVQL
                     LKREEELRRKEQEDTERSRAERRAEQLRKNDNAVMDSGVLSAVVTGYGEAPMPGNGEMT
                     CYLLLHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQHPPLQRVYSSA
                     TFEQLLTQVCQTWPQYTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLLPTL
                     MPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKG
                     LRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRT
                     WDEWRREEDARTHATERRHDRGWSQ"
                     /name="mobQ"
     gene            complement(4343..5044)
                     /locus_tag="U13A_RS26800"
                     /old_locus_tag="U13A_D00005"
     CDS             complement(4343..5044)
                     /locus_tag="U13A_RS26800"
                     /old_locus_tag="U13A_D00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001281397.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCIGRLKSGADAESPKL
                     QKTMTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKDDEKREEFRWIQYRAQY
                     AKGEARAQITFSDAVMPYLTQLQGQFTRVVIKNISRLSRSYSIRIYELLQQFRSTGERI
                     IALDDFRSMLDIEHKYQTYKSLNQQLLRPCIDELNKKSDLAVTVETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(5577..5831)
                     /gene="mobC"
                     /locus_tag="U13A_RS26810"
                     /old_locus_tag="U13A_D00006"
     CDS             complement(5577..5831)
                     /gene="mobC"
                     /locus_tag="U13A_RS26810"
                     /old_locus_tag="U13A_D00006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091745.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVIVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPGQ"
                     /name="mobC"
     gene            6010..>8154
                     /gene="mobQ"
                     /locus_tag="U13A_RS26815"
                     /old_locus_tag="U13A_D00007"
                     /pseudo=""
     CDS             6010..>8154
                     /gene="mobQ"
                     /locus_tag="U13A_RS26815"
                     /old_locus_tag="U13A_D00007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /name="mobQ"
     Typing          complement(1751..1927)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          1928..2286
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(5833..6009)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          6010..6368
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(260..962)
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.716"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(4344..5044)
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          1842..4084
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.866"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          5924..8004
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.856"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 agtgaatgtg aacaatattt tgttttgttt aacatcgtgt tatgttcgtc gatgttcttc
       61 gatgttcatc gacagggatg aatgcaagaa gcacacacat aatgcaagca tagttttgtt
      121 tttccgcccc caaaaaatgg gggttctttt ttcgctgcat tagagcgctt ttgacgcaat
      181 tttcacttca atagtacctt cgcactacta acacaaaaaa acgcagcagg gagcgtttta
      241 ggtgccttaa aatcgatttt agatcgtcat cttgatttgt ttgtcttcct tgaagcgaaa
      301 atgcagggct acaaccgtgc gccccttttt gatcgtctca accgttacag ccaggtcgct
      361 ttttttgttc agttcgtcaa tgcagggtct taataattgc tggttaagtg atttatatgt
      421 ttgatattta tgctctatat caagcattga gcgaaaatca tcgagagcga taatacgttc
      481 gccagtactg cggaactgct ggaggagttc gtatatcctt atactgtaac ttcttgataa
      541 tctagatata ttcttgatta caactctagt aaattgccct tgtagttgtg tcaaataagg
      601 cattactgcg tcagaaaatg ttatttgtgc tctggcctcg cctttagcgt actgtgcccg
      661 atactggact ccagcggaac tcctcacgtt tctcgtcatc cttgagaatg attgacctgt
      721 cccacaaacg atcaattgct tcctgtaact gaacctcggc attttttccg ccccatgtca
      781 gggaatgaat cgaaatattc cgcagctgta atggtcattg tcttttgcaa ttttggagat
      841 tctgcatcag ccccagactt caatcgaccg atgcaaagta acaaaaatcg ctgttcctgt
      901 agggtcaatt tataactggc ttcaaccagc gcattggatt tataggcaat tagatccgac
      961 atagagcatc cctccccaaa aggtaatgaa taaaagataa acataccctc aacgaaaggc
     1021 aatgcctttt tattttaata tccctttttc tgtgaatatt accttaaatc atattgcctt
     1081 taccttaaat tcccccacct aaacacctta aatcatatta cctttacctt aaatcacatt
     1141 acctatagca ttgtaacatt ttgttttcta taaggaattt tggcgctaaa acaagtatta
     1201 aaaacaagat cttaaaaaca agatctaaaa acttaacgac gagcctgtgg ataacttgga
     1261 gtactgaaaa caaaaaacca ataaaaacaa tgcgttgcaa aaaagcagaa ttgcaagttc
     1321 cgaatacacc ccctccaacc ctgtggaaat ccagtattca tgcgggttca gcgcatatca
     1381 cctcccgaaa aaccgtgcgt atgtgctcaa tcaggcgacg gattcacttc cgcttcctcg
     1441 cccactcgct cgctaacgct cggtcgctgg gactgcggcg agcggacgat acaatcactg
     1501 tcctggcagc gcttccagct cgttaatcag cggcagtatg cgcgtcacat cagcctgtcg
     1561 tgtcacctct gccttcagga actggagtaa ccaggcacgc aactgcacct cctccggctt
     1621 tctgaccttt gccagcaatg ctgcacccac aatgaccttc tgcgctgtgt ccagactcct
     1681 gctcttcgcc ttcaggcgct gtaatctggc ctcagcatca gcaatcttct gttcgagtgt
     1741 tctgctcatg atgtaactcc gtgcgcgatg aaaaatcgca ttttagcgcg tcactggtag
     1801 tttaaaaact gagctggcat aatgcacgga aaatcacgaa gtgcgcactt atacaatctc
     1861 cactccgttt cgattgtgtg cgccctgcgg ggctgaaaga aaaccacaaa aaggcattac
     1921 ggcagaaatg gcgatttatc atctcagcat gaaaatcatc tccagaaaca gcggctacag
     1981 cgccgttgct tctgctgcct atcgttccgg ttcactgatg cttgatgagc gtaccggact
     2041 gactcacgac tacacccgca aaagcggtgt ggctgaagct gtgattctga ctcccgcgac
     2101 agcccctgcc tggtgtacga accgtgccga actctggaat gccgttgaga aggccgaacg
     2161 ccggaagaac tcacaacttg cgcgggaaat tgaactcgcc atcccccgcg aactaccgca
     2221 ggatgccgca cgggaaaccg ttctcgcctt tgtcagagaa aactttgtca gtcagggcat
     2281 gattgctgat gtggcgttcc atcacatgga taaaacaaat ccccatgcgc acatcatgct
     2341 gaccacgaga gctgtcggac ctgctggatt cggtggaaag gtcagggact ggaacgaccg
     2401 gacacatgcc gagacgtggc gtgcatcatg ggctgaccat gcgaacaggg cactggcgaa
     2461 cgccggttat caggaagaga tagaccatcg ttcatacgag cgacaggggc ttgagaaaac
     2521 gcctggtatt cacctcggaa agtctgcctg tgcgatggaa acacgcggta tcgaaacaga
     2581 acgcggcgaa cagaatcgtc tgattaacag acttaacctg gaaatacaga tttcccgtac
     2641 cgaactcaga aacagcgggc tgacagagcc tgcccgccgt gtggctgacc ttctgaatat
     2701 cgtgcttcct gacaacgcaa cttcgtacac actacgtgac atcatcgaag cgttgccgaa
     2761 ggacagtaat gcggcatgga aactcaccag taatgccatg tcgatgatgg cagatatgca
     2821 ggcgacgcgc agacgctggg aagagctgaa caccgaacga aaggaagcga tgagccatgc
     2881 tgccagcctg aaaaaatcat cgccgttttc gtccggtttt tcccgtattc ccctgatgca
     2941 atgggcggca ccggaatacc gtagcgaaca gggaaaaatc cagtcgctga gccagcaaat
     3001 ggaaaaattg cgacagcatt accggaaggt ggagaaacag gacattcccg cgagtcagac
     3061 ggcatttgaa aaacagtggc agcagtgggg ggcatccggc cttgcagctc tcagagtcca
     3121 gttgcttaaa cgtgaagagg aattgcgccg gaaggagcag gaggatacag aacgtagtcg
     3181 cgcggagcga cgagcggaac agctcaggaa aaatgacaat gccgttatgg attcaggggt
     3241 actgagcgca gtcgttacgg ggtatggcga ggcccccatg cctggtaacg gggaaatgac
     3301 ctgttacctt ctgcttcaca accggaacgg agaatacacg ctctggggga acgagctgga
     3361 gaaataccgc accagaatct ttgagcgtgt tgatttaatg cgtgacagga gtggttatat
     3421 ctgtgaccga agcgaaatcg gtcaacatcc accactgcaa cgtgtttatt cctctgcgac
     3481 ctttgagcaa cttctgacgc aggtttgcca gacatggccg caatataccc gcaatctccg
     3541 ccagccgaag acatggccgg agtctttctg tcttggagaa gaccgccagc cagccatgcc
     3601 atcactggca gcaaggaagg tggattttac gcagggacgt ctgctaccga cgctgatgcc
     3661 ggtaatgagt tctgtcgacc gggaaacccg ccagcttcag cttttgctgg tcatgggcgt
     3721 ggatgactct ctgggcggtg ttgtccggct taatggtacg ctgtatcccg cgtttgccgt
     3781 tccctcagcc gataacagcc agctggtcat cagtgcactg accgataagg ggttacggta
     3841 tgcagggtat ggtgttgccg taaatcacga tgcagacagc cacatatcac ctgcgccgga
     3901 acttatggag tttcatctga aaacccgtga ggcaccgctt tttgctgccg tcaacacacc
     3961 agaaaaacag ccggatcatc tgttccgctc gctgggtttt aatcgtacct gggacgaatg
     4021 gcggcgcgag gaagatgcac gaacacacgc caccgagagg agacatgatc gcggttggtc
     4081 acagtgaatg tgaacaatat tttgttttgt ttaacatcgt gttatgttcg tcgatgttct
     4141 tcgatgttca tcgacaggga tgaatgcaag aagcacacac ataatgcaag catagttttg
     4201 tttttccgcc cccaaaaaat gggggttctt ttttcgctgc attagagcgc ttttgacgca
     4261 atttttcact tcaatagtac cttcgcacta ctaacacaaa aaaaacgcag cagggagcgt
     4321 tttaggtgcc ttaaaatcga ttttagatcg tcatcttgat ttgtttgtct tccttgaagc
     4381 gaaaatgcag ggctacaacc gtgcgcccct ttttgatcgt ctcaaccgtt acagccaggt
     4441 cgcttttttt gttcagttcg tcaatgcagg gtcttaataa ttgctggtta agtgatttat
     4501 atgtttgata tttatgctct atatcaagca ttgagcgaaa atcatcgaga gcgataatac
     4561 gttcgccagt actgcggaac tgctggagga gttcgtatat ccttatactg taacttcttg
     4621 ataatctaga tatattcttg attacaactc tagtaaattg cccttgtagt tgtgtcaaat
     4681 aaggcattac tgcgtcagaa aatgttattt gtgctctggc ctcgccttta gcgtactgtg
     4741 cccgatactg gatccagcgg aactcctcac gtttctcgtc atccttgaga atgattgacc
     4801 tgtcccacaa acgatcaatt gcttcctgta actgaacctc ggcatttttc cgccccatgt
     4861 cagggaatga atcgaaatat tccgcagctg taatggtcat tgtcttttgc aattttggag
     4921 attctgcatc agccccagac ttcaatcgac cgatgcaaag taacaaaaat cgctgttcct
     4981 gtagggtcaa tttataactg gcttcaacca gcgcattgga tttataggca attagatccg
     5041 acatagagca tccctcccca aaaggtaatg aataaaagat aaacataccc tcaacgaaag
     5101 gcaatgcctt tttattttaa tatccctttt tctgtgaata ttaccttaaa tcatattgcc
     5161 tttaccttaa attcccccac ctaaacacct taaatcatat tacctttacc ttaaatcaca
     5221 ttacctatag cattgtaaca ttttgttttc tataaggaat tttggcgcta aaacaagtat
     5281 taaaaacaag atcttaaaaa caagatctaa aaacttaacg acgagcctgt ggataacttg
     5341 gagtactgaa aacaaaaaac caataaaaac aatgcgttgc aaaaaagcag aattgcaagt
     5401 tccgaataca ccccctccaa ccctgtggaa atccagtatt catgcgggtt cagcgcatat
     5461 cacctcccga aaaaccgtgc gtatgtgctc aatcaggcga cggattcact tccgcttcct
     5521 cgcccactcg ctcgctaacg ctcggtcgct gggactgcgg cgagcggacg atacaatcac
     5581 tgtcctggca gcgcttccag ctcgttaatc agcggcagta tgcgcgtcac atcagcctgt
     5641 cgtgtcacct ctgccttcag gaactggagt aaccaggcac gcaactgcac ctcctccggc
     5701 tttctgacct ttgccagcaa tgctgcaccc acaatgacct tctgcgctgt gtccagactc
     5761 ctgctcttcg ccttcaggcg ctgtaatctg gcctcagcat cagcaatctt ctgttcgagt
     5821 gttctgctca tgatgtaact ccgtgcgcga tgaaaaatcg cattttagcg cgtcactggt
     5881 agtttaaaaa ctgagctggc ataatgcacg gaaaatcacg aagtgcgcac ttatacaatc
     5941 tccactccgt ttcgattgtg tgcgccctgc ggggctgaaa gaaaaccaca aaaaggcatt
     6001 acggcagaaa tggcgattta tcatctcagc atgaaaatca tctccagaaa cagcggctac
     6061 agcgccgttg cttctgctgc ctatcgttcc ggttcactga tgcttgatga gcgtaccgga
     6121 ctgactcacg actacacccg caaaagcggt gtggctgaag ctgtgattct gactcccgcg
     6181 acagcccctg cctggtgtac gaaccgtgcc gaactctgga atgccgttga gaaggccgaa
     6241 cgccggaaga actcacaact tgcgcgggaa attgaactcg ccatcccccg cgaactaccg
     6301 caggatgccg cacgggaaac cgttctcgcc tttgtcagag aaaactttgt cagtcagggc
     6361 atgattgctg atgtggcgtt ccatcacatg gataaaacaa atccccatgc gcacatcatg
     6421 ctgaccacga gagctgtcgg acctgctgga ttcggtggaa aggtcaggga ctggaacgac
     6481 cggacacatg ccgagacgtg gcgtgcatca tgggctgacc atgcgaacag ggcactggcg
     6541 aacgccggtt atcaggaaga gatagaccat cgttcatacg agcgacaggg gcttgagaaa
     6601 acgcctggta ttcacctcgg aaagtctgcc tgtgcgatgg aaacacgcgg tatcgaaaca
     6661 gaacgcggcg aacagaatcg tctgattaac agacttaacc tggaaataca gatttcccgt
     6721 accgaactca gaaacagcgg gctgacagag cctgcccgcc gtgtggctga ccttctgaat
     6781 atcgtgcttc ctgacaacgc aacttcgtac acactacgtg acatcatcga agcgttgccg
     6841 aaggacagta atgcggcatg gaaactcacc agtaatgcca tgtcgatgat ggcagatatg
     6901 caggcgacgc gcagacgctg ggaagagctg aacaccgaac gaaaggaagc gatgagccat
     6961 gctgccagcc tgaaaaaatc atcgccgttt tcgtccggtt tttcccgtat tcccctgatg
     7021 caatgggcgg caccggaata ccgtagcgaa cagggaaaaa tccagtcgct gagccagcaa
     7081 atggaaaaat tgcgacagca ttaccggaag gtggagaaac aggacattcc cgcgagtcag
     7141 acggcatttg aaaaacagtg gcagcagtgg ggggcatccg gccttgcagc tctcagagtc
     7201 cagttgctta aacgtgaaga ggaattgcgc cggaaggagc aggaggatac agaacgtagt
     7261 cgcgcggagc gacgagcgga acagctcagg aaaaatgaca atgccgttat ggattcaggg
     7321 gtactgagcg cagtcgttac ggggtatggc gaggccccca tgcctggtaa cggggaaatg
     7381 acctgttacc ttctgcttca caaccggaac ggagaataca cgctctgggg gaacgagctg
     7441 gagaaatacc gcaccagaat ctttgagcgt gttgatttaa tgcgtgacag gagtggttat
     7501 atctgtgacc gaagcgaaat cggtcaacat ccaccactgc aacgtgttta ttcctctgcg
     7561 acctttgagc aacttctgac gcaggtttgc cagacatggc cgcaatatac ccgcaatctc
     7621 cgccagccga agacatggcc ggagtctttc tgtcttggag aagaccgcca gccagccatg
     7681 ccatcactgg cagcaaggaa ggtggatttt acgcagggac gtctgctacc gacgctgatg
     7741 ccggtaatga gttctgtcga ccgggaaacc cgccagcttc agcttttgct ggtcatgggc
     7801 gtggatgact ctctgggcgg tgttgtccgg cttaatggta cgctgtatcc cgcgtttgcc
     7861 gttccctcag ccgataacag ccagctggtc atcagtgcac tgaccgataa ggggttacgg
     7921 tatgcagggt atggtgttgc cgtaaatcac gatgcagaca gccacatatc acctgcgccg
     7981 gaacttatgg agtttcatct gaaacccgtg agcaccgctt tttgctgccg tcaacacacc
     8041 agaaaaacag ccggatcatc tgttccgctc gctgggtttt aatcgtacct gggacgaatg
     8101 gcggcgcgag gaagatgcac gaacacacgc caccgagaga gacatgatcg cggttgtcac
     8161 agtgaa
//
