LOCUS       NZ_CP035370             3125 bp    DNA     circular CON 25-AUG-2023
DEFINITION  Staphylococcus aureus strain LAC plasmid unnamed, complete sequence.
ACCESSION   NZ_CP035370
VERSION     NZ_CP035370.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN10779666
            Assembly: GCF_004118995.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Monk,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2019) Bioengineering, UC San Diego, 9500 Gilman
            Drive, La Jolla, CA 92093, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: JUN-2017
            Assembly Method                   :: Unicycler v. 0.4.2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50.0x
            Sequencing Technology             :: Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/25/2023 02:18:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,901
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,732
            CDSs (with protein)               :: 2,732
            Genes (RNA)                       :: 90
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 79
            CDSs (without protein)            :: 79
            Pseudo Genes (ambiguous residues) :: 0 of 79
            Pseudo Genes (frameshifted)       :: 44 of 79
            Pseudo Genes (incomplete)         :: 40 of 79
            Pseudo Genes (internal stop)      :: 14 of 79
            Pseudo Genes (multiple problems)  :: 18 of 79
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP035370.1.
            Bacteria and source DNA available from Adam Feist.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="LAC"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="unnamed"
                     /geo_loc_name="USA: San Diego"
                     /lat_lon="32.71 N 117.16 W"
                     /collection_date="2017-06"
                     /collected_by="San Diego"
     gene            complement(join(2629..3125,1..85))
                     /locus_tag="ERW10_RS15145"
                     /old_locus_tag="ERW10_14450"
     CDS             complement(join(2629..3125,1..85))
                     /locus_tag="ERW10_RS15145"
                     /old_locus_tag="ERW10_14450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000991705.1"
                     /translation="MNAIQNSISLRKILVVSLSILTILSMVLDFNFKEAQAQNKNNIAD
                     KNVETLNEKEIEKELKYIYGKIIILDKDGTAKDVNLENAKSRYGYVPEGFQKLKNDIEN
                     KKKTSSISPRAVGGNYKNSGDCFYSEVLNSYGELLTGNIITAVFEDVKAKNLKGVAKKL
                     AKIGIKGNLAGIAATMVSKDVQCNLKYGLL"
                     /name="hypothetical protein"
     gene            537..1532
                     /locus_tag="ERW10_RS15150"
                     /old_locus_tag="ERW10_14455"
     CDS             537..1532
                     /locus_tag="ERW10_RS15150"
                     /old_locus_tag="ERW10_14455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001199892.1"
                     /translation="MQYNTTRSITENQDNKTLKDMTKSGKQRPWREKKIDNVSYADILE
                     ILKIKKAFNVKQCGNILEFKPTDEGYLKLHKTWFCKSKLCPVCNWRRAMKNSYQAQKVI
                     EKVIKEKPKARWLFLTLSTKNAIDGDTLEQSLKHLTKAFDRLSRYKKVKQNLVGFMRST
                     EVTVNKNDGSYNQHMHVLLCVENAYFRKKENYITQEEWVNLWQRALQVDYRPVANVKAI
                     KPNRKGDKDIESAIKETSKYSVKSSDFLTDDDEKNQEIVSDLEKGLYRKRMLSYGGLLK
                     QKHKILNLDDVEDGNLINASDEDKTTDEEEKAHSITAIWNFEKQNYYLRH"
                     /name="protein rep"
     gene            2115..2303
                     /locus_tag="ERW10_RS15160"
                     /old_locus_tag="ERW10_14460"
     CDS             2115..2303
                     /locus_tag="ERW10_RS15160"
                     /old_locus_tag="ERW10_14460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001039248.1"
                     /translation="MNKNTVLDEGYVIATILNVFFLLGLIFISHLENLYILIPYVILMG
                     INAIYLVVKVMNFKKNN"
                     /name="hypothetical protein"
     Typing          1603..1767
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.42e-81"
     Typing          538..1532
                     /name="rep_cluster_1017"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.9"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ccaagaccat tgaaagtatg gttaaaatac ttaaagatac tactaaaatc tttcttagtg
       61 atatactgtt ttgaattgca ttcattgtaa atgccccctt gttttgttat ttaaatgaat
      121 tcaatataat attaacatat aaaaacaaag gagtttaata tgaataagtt tttttacttt
      181 ttgattattt tattatctgt atttttaatt tttaattttg ttaattcgat tttatttgat
      241 aatccttttg actttttaga aagttttatt tattcattgg ttttcacatt aatttattta
      301 ggtttagagg gtcataaaaa gaagaaggaa taggttgttt tttgaaacga gtgtgaacga
      361 gtttcttctt gtcttgatac tatatagaaa taactcgatt ttatatatat agctgtaact
      421 gttgatatta cagtgtttaa acgtgttttt gtgcgtgaaa ggaaaatttg acaataaaaa
      481 accccagtta tattattaag gtgtcgaatc ttaaataata ctgggggtct ttttatatgc
      541 aatataatac tactagaagt ataaccgaaa atcaagataa taaaacgtta aaagatatga
      601 cgaaaagtgg gaaacaacgc ccatggagag aaaagaaaat agataatgta agctatgcag
      661 atatactaga aattttaaaa atcaaaaagg cttttaatgt aaaacaatgt ggtaatattt
      721 tagaatttaa gccaactgat gaaggctatt tgaagttaca taagacatgg ttttgtaaat
      781 caaaattatg tccggtttgt aattggagac gtgctatgaa aaatagttat caagctcaaa
      841 aagtgattga aaaagtaatt aaggaaaagc caaaagcacg ttggttgttt ttaacacttt
      901 caacaaaaaa tgcgatagat ggagatactt tagaacaaag tttgaagcat ctaactaaag
      961 catttgatag gttgagtaga tataaaaagg ttaaacaaaa tcttgttgga tttatgcgtt
     1021 caacagaagt taccgttaat aaaaatgacg gtagttataa tcagcacatg catgttttgt
     1081 tatgtgttga aaatgcatat tttagaaaaa aagagaatta tataactcaa gaagaatggg
     1141 ttaatttatg gcaaagagca ttacaagttg actatcgacc tgttgctaat gttaaagcga
     1201 tcaaaccgaa tagaaaaggc gataaagaca ttgaatcggc aatcaaagag acctcaaaat
     1261 attcggttaa atcatctgat tttttaactg atgatgatga aaaaaatcaa gaaattgtaa
     1321 gtgatttaga aaaaggtttg tatcgaaaac gtatgttaag ttatggtgga ttgcttaaac
     1381 aaaaacataa aattttaaac ttagacgatg tcgaagatgg taatttgatt aatgcaagtg
     1441 atgaagataa aacaacagac gaagaagaaa aagcacattc aattaccgca atttggaatt
     1501 tcgaaaagca aaattattat ttaagacatt agtgttgact aatgtctttt ttgttgattt
     1561 tttataaaaa agtactgtct tatttttgtg acaaatgctg tatgtagtgt cacaaaaata
     1621 agacaaacgc aatatattgt gtcacaaaaa taagacagta cagctttgta tgatatcact
     1681 ttaaaatacc ttaaaacccc tggaatttct ggctttgcca gacctatcat ttttgaatga
     1741 tagcaaattc tccttatgct cttacggagt ttttagagaa aaattaaaaa ttctcgattt
     1801 ttgataaaaa acgccctgca ggaatttaga aaaacatgtg gaagttttaa aggattttat
     1861 gctaattttt aatttgcatg taaatgggca gtgttaaaaa attagtcact ggttttgctg
     1921 aaatttttgt ttgtgaaatt accatatatt tatttggctc atatttgcgt tttaagagct
     1981 tgtatatatt tttaagttaa aaacctatat gataccccct aaacctttaa aataccccct
     2041 taaaactcaa aataaaggta tttataattt gagtaagtct tgtgataaag ttttttaaaa
     2101 aaggagtgtt tattttgaat aaaaacacag tgttagatga aggttacgtt atagcaacta
     2161 ttttaaatgt tttcttttta cttggattaa tttttatatc tcatttggaa aatttatata
     2221 tattaattcc gtatgttatt ttgatgggaa taaatgctat ttatttggtt gttaaggtta
     2281 tgaatttcaa aaagaataat tagaatattt gaggggattt tgaaacgagt ttcttcttgt
     2341 tttcacactg tttttttatt cctattggtg ttgttgctta ctttttgttt ttctataaag
     2401 atgatgcttt tgacgagatg gaagaaaaat atgattatca tgaagataat aaaaaataga
     2461 cgacgcattt atgccgagaa aatttattga tgttgagaag aacccttaac taaacttgca
     2521 gacgaatgtc ggcatagcgt gagctattaa gccgaccatt cgacaagttt tgggattgtt
     2581 aagggttccg aggctcaacg tcaataaagc aattggaata aatagtatct aaagtaaccc
     2641 gtatttaaga ttacattgta catccttcga aaccatagta gctgcgattc cagctaggtt
     2701 tccttttatc ccaattttag ctaatttttt tgctacgccc tttaagtttt tggctttaac
     2761 atcttcaaaa actgctgtga ttatgtttcc agttaaaagt tctccatatg agtttaaaac
     2821 ttcagaatag aaacagtcac cgctattttt gtagttccca ccaactgctc taggtgatat
     2881 tgaactagtt ttctttttat tctctatgtc gttttttagt ttttggaatc cttcaggaac
     2941 atatccatat cgagatttgg cattttctag attaacatct ttagctgttc cgtctttatc
     3001 taaaataata attttgccat atatgtattt tagttctttt tcaatttctt tttcgtttaa
     3061 agtttcaacg tttttatctg caatgttatt tttgttttgg gcttgagctt ctttgaaatt
     3121 aaaat
//
