LOCUS       NZ_CP035290             4439 bp    DNA     circular CON 23-APR-2024
DEFINITION  Staphylococcus epidermidis strain ATCC 14990 plasmid unnamed2,
            complete sequence.
ACCESSION   NZ_CP035290
VERSION     NZ_CP035290.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN10738425
            Assembly: GCF_006094375.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus epidermidis
  ORGANISM  Staphylococcus epidermidis
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4439)
  AUTHORS   Chen,H. and Liang,Q.
  TITLE     Staphylococcus epidermidis Genome sequence and assembly
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4439)
  AUTHORS   Chen,H. and Liang,Q.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2019) Key Laboratory of Microbial Technology and
            Bioinformatics of Zhejiang Province, Zhejiang Tianke High Technology
            Development Co.Ltd., Huanggushan Road, Hangzhou, Zhejiang 310000,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 240.0x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_006094375.1-RS_2024_04_23
            Annotation Date                   :: 04/23/2024 01:42:59
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,368
            CDSs (total)                      :: 2,284
            Genes (coding)                    :: 2,231
            CDSs (with protein)               :: 2,231
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 34 of 53
            Pseudo Genes (incomplete)         :: 31 of 53
            Pseudo Genes (internal stop)      :: 24 of 53
            Pseudo Genes (multiple problems)  :: 28 of 53
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP035290.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4439
                     /organism="Staphylococcus epidermidis"
                     /mol_type="genomic DNA"
                     /strain="ATCC 14990"
                     /culture_collection="ATCC:14990"
                     /type_material="type strain of Staphylococcus epidermidis"
                     /db_xref="taxon:1282"
                     /plasmid="unnamed2"
     ARG             complement(2..357)
                     /name="tet(K)"
                     /gene="tet(K)"
                     /gene_name="major facilitator superfamily (MFS) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="tetracycline antibiotic"
                     /identity="100.0"
                     /coverage="25.93"
     ARG             complement(3418..4439)
                     /name="tet(K)"
                     /gene="tet(K)"
                     /gene_name="major facilitator superfamily (MFS) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="tetracycline antibiotic"
                     /identity="100.0"
                     /coverage="74.07"
     gene            complement(519..1463)
                     /locus_tag="EQW00_RS12265"
                     /old_locus_tag="EQW00_12280"
                     /db_xref="GeneID:50019749"
     CDS             complement(519..1463)
                     /locus_tag="EQW00_RS12265"
                     /old_locus_tag="EQW00_12280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277654.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_000277654.1"
                     /db_xref="GeneID:50019749"
                     /translation="MYKNNHANHSNHLENHDLDNFSKTGYSNSRLDAHTVCISDPKLSF
                     DAMTIVGNLNRDNAQALSKFMSVEPQIRLWDILQTKFKAKALQEKVYIEYDKVKADSWD
                     RRNMRIEFNPNKLTRDEMIWLKQNIISYMEDDGFTRLDLAFDFEDDLSDYYAMSDKAVK
                     KTIFYGRNGKPETKYFGVRDSNRFIRIYNKKQERKDNADAEVMSEHLWRVEIELKRDMV
                     DYWNDCFSDLHILQPDWKTIQRTADRAIVFMLLSDEEEWGKLHRNSRTKYKNLIKEISP
                     VDLTDLMKSTLKANEKQLQKQIDFWQHEFKFWK"
                     /name="replication initiation factor domain-containing
                     protein"
     gene            complement(1990..3231)
                     /gene="mobV"
                     /locus_tag="EQW00_RS12270"
                     /old_locus_tag="EQW00_12285"
                     /db_xref="GeneID:50019750"
     CDS             complement(1990..3231)
                     /gene="mobV"
                     /locus_tag="EQW00_RS12270"
                     /old_locus_tag="EQW00_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010889940.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_000122374.1"
                     /db_xref="GeneID:50019750"
                     /translation="MSYSIVRVSKVKSGTNTTGIQKHVQRENNNYENEDIDHSKTYLNY
                     DLVNANKQNFNNLIDEKIEQNYTGKRKIRTDAIKHIDGLITSDNDFFDNQTPEDTKQFF
                     EYAKEFLEQEYGKDNLLYATVHMDEKTPHMHYGVVPITDDGRLSAKEVVGNKKALTAFQ
                     DRFNEHVKQRGYDLERGQSRQVTNAKHEQISQYKQKTEYHKQEYERESQKTDHIKQKND
                     KLMQEYQKSLNTLKKPINVPYEQETEKVGGLFSKEIQETGNVVISQKDFNEFQKQIKAA
                     QDISEDYEYIKSGRALDDKDKEIREKDDLLNKAVERIENADDNFNQLYENAKPLKENIE
                     IALKLLKILLKELERVLGRNTFAERVNKLTEDEPKLNGLAGNLDKKMNPELYSEQEQQQ
                     EQQKNQKRDRGMHL"
                     /name="mobV"
     Typing          complement(520..1463)
                     /name="rep_cluster_1118"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(1994..3231)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tgcagcagat cctactcctt gtactaacct accaaaaatc aaaataaaaa agtgattgtg
       61 accaataaaa gcaatcaatg aaccaagaca gctcaaacta ataccaataa ttaacaattt
      121 ttttatattt atataatcag ataattttcc atatactgct gttcctatcg aaaaagttaa
      181 catatatgca gtgtttaccc agtttgtaat tccaggagta gtattaaaat gatttgcaat
      241 atcaggtaaa gaaacattta aaaccatttc atttaataca ctaaaaaatg aaagaataca
      301 aagccaaaat aaaacgctat aaaacaaacc tttaaatttt ttatataaac taaacaattt
      361 tacctcttta cttgaggtga ctaaagttta taggggtaaa ttattctttc acctcatata
      421 aattccccaa atttttaaat agacacttca taaaaaaatc ctcctaaatt agaatagttt
      481 aaatatatca tttttgttca gtaatattaa tatgtacact atttccaaaa tttaaattca
      541 tgttgccaaa aatcgatttg tttttgcaat tgtttttcgt tcgcttttaa agtcgatttc
      601 attaagtccg ttaaatcgac tggcgaaatt tcttttatca aattcttata ttttgttcta
      661 gaatttctgt gaagctttcc ccattcttct tcatcactca ataacataaa aactattgct
      721 ctatccgcag tgcgttggat agttttccaa tctggttgca agatatgtaa atcactaaag
      781 caatcattcc agtaatccac catatctctt ttaagttcga tttctacacg ccataaatgt
      841 tcagacataa cttcagcatc tgcattatct ttacgttctt gctttttatt ataaattcta
      901 ataaatctat tactatctct cacgccaaaa tattttgttt ctggcttacc attacgacca
      961 taaaaaatag ttttcttaac tgctttatca gacattgcat agtagtcact caaatcatct
     1021 tcaaaatcaa aggctaaatc taatcttgta aaaccgtcat cttccatgta gcttattata
     1081 ttttgtttta accaaatcat ttcatctcgt gtaagtttgt ttggattaaa ttcaatacgc
     1141 atattacgtc tatcccaact atctgctttc actttgtcat attcaatata aactttttct
     1201 tgaagtgctt tagctttaaa ctttgtttga agaatatccc aaagtcttat ttggggctct
     1261 acactcataa atttagaaag ggcttgagcg ttgtctcggt tgagatttcc aacgatcgtc
     1321 attgcatcaa aacttaattt tggatcagat atgcacacag tatgtgcgtc caaccggcta
     1381 ttagagtagc cggttttaga aaaattgtct aaatcgtgat tttccaaatg atttgaatga
     1441 tttgcatgat tgtttttata cataaaaaat cgactcctta atctcaattt cgtttaagga
     1501 atcgctcacc caaatatata tcttgatgta tatttaaata tcgtttaata tctaaatata
     1561 caagattata aaaacaactc agtgtttttt tctttgaatg atgtcgttca caaactttgg
     1621 tcagggcgtg agcgactcct ttttattttg ttattaatat aacactatca aaagacttgg
     1681 tctaatcaga tcaagtcttt ttttatttaa gcatttgtat tatctggtaa acagttaaaa
     1741 ctactaaaac accaagtaca tacttacttg ttataaaatt ctcaaaggct tctctgaatt
     1801 tcatttcttc aactcctttt attgctttat tccaatttcc ttattggtcg gaacctacag
     1861 ggctttatgt gttgttgagt tggtactttc ttgggattaa tcccaattca agtccaacca
     1921 actcgctaac aagttagcta acacatagcc cattccaacc aataagtttt ctcggcataa
     1981 atgcatgttc tataagtgca tacctctatc tcgtttttga ttcttttgtt gttcttgttg
     2041 ctgttcctgt tctgaatata attctggatt cattttttta tctaagtttc ctgctaaacc
     2101 atttagtttt ggttcatctt ctgttaactt attaactctt tccgcaaagg tatttcttcc
     2161 taaaactcgt tctaactctt ttagtaagat ttttaaaagc tttaacgcta tttctatatt
     2221 ctctttaagt ggctttgcat tttcgtaaag ttggttaaaa ttatcgtctg cgttttcaat
     2281 acgctcaact gctttattta ataaatcatc tttctctcgt atttccttat ctttatcatc
     2341 taaggctcta ccagacttta tatactcgta atcttccgaa atatcttgag cagcttttat
     2401 ctgtttctga aattcattga aatctttttg gcttattaca acatttccag tttcttgtat
     2461 ttctttgcta aataaaccac ctactttttc agtttcttgc tcatacggaa catttatagg
     2521 ctttttaagc gtatttaacg atttttggta ctcttgcatt aatttatcgt tcttttgctt
     2581 tatatggtct gttttttggc tctcacgttc atattcttgc ttatgatatt ctgttttttg
     2641 tttatactga cttatttgct catgtttagc atttgttact tgtcttgatt gcccacgttc
     2701 taaatcatat cctcgttgtt taacatgctc attaaatcta tcttgaaacg ctgttaaagc
     2761 ttttttatta cctacaactt ctttagcact taaacgacca tcatcagtta ttggaacaac
     2821 gccataatgc atatgtggtg ttttttcgtc catgtgaact gttgcatata ataaattatc
     2881 tttaccgtat tcttgttcta aaaactcttt agcatattca aaaaactgct ttgtatcttc
     2941 tggcgtttga ttatcaaaga aatcattgtc tgatgtaatt aaaccatcaa tgtgtttaat
     3001 cgcgtctgtt ctaatttttc ttttgcctgt ataattctgt tcgatttttt catcaatcaa
     3061 gttattaaaa ttctgtttat tagcatttac caaatcataa tttaagtaag ttttactatg
     3121 gtctatatct tcattttcat aattattatt ttctctttga acatgttttt gtatgcccgt
     3181 tgtatttgtt ccagatttaa cttttgaaac tctaacaatg gaataagaca tataaaaatc
     3241 actcctaagc acaaaggttt catgtgtatt taatagttta agtctaacag actagactta
     3301 tttttaataa gtcgttaata cgtgtgctct gcgaggctta aacctgtttt tgctaacgca
     3361 aaaaatgagt ggcaaaatgc tagccactca tagttctaaa ccaaaatata atataactat
     3421 tcaaactgct tttcagaacg tttaaataca ataatcgtca aaagacaaca taaaataata
     3481 aggatagcca tggctacaag aatattacta tacactccag aagaataatt tataaattcc
     3541 agaactagtt tacgattaat caattgtagt gacaataaac ctcctacaat tgctatacct
     3601 gttccctctg ataaaaaact tgtgaaattt agcaaactca ttccagaagc aacttcttct
     3661 tcagaaagac tacttgatac tatttttgat ataactgttt tagtaaaaga taatccgccc
     3721 ataacaaata taaacataaa agtagtcaac cacatactaa actcaacaaa aaatgcaata
     3781 gttaaaaaac ttatagagat agacaatgat cctaaaataa aaacaaataa tgatcctttt
     3841 ctatccacta aaaaaccacc aaaataacca aaaacaataa cactcatggt tccaggaaaa
     3901 ataacactat tacctattgt cgctacattt acatgataaa tagttttcat catataaggc
     3961 accattgata taaaaccagc tactatagaa aatattagcc caccagaaaa caaaccaagc
     4021 ataaacggaa tgtttttccc tagtttagga ttaataaaag ggttagaaac tcttgaaata
     4081 tgtttaataa aaatcacaaa aaagattgtg aagagtatta aaaaagtcca attataattt
     4141 gtcgtaaata acataaaaca tataatactt atagacatta aaacaatacc tacgatatct
     4201 aatgtatttt ttgttgattt accaggtacc attactttaa taagaaaagg tatagttact
     4261 attgtaatca taggaagtat aagtaggtaa gaccaatgaa tataatgtgc tattattccc
     4321 cctattgaag gacctaaccc ttcacctaaa gctacaattg atcctataaa accaaaggct
     4381 ttgccttgtt tttttcttgt aatatttcta gctacaacca ccataatcag tgaagggaa
//
