LOCUS       NZ_CP035206             6364 bp    DNA     circular CON 26-NOV-2023
DEFINITION  Klebsiella pneumoniae strain LH94 plasmid pLH94-2, complete
            sequence.
ACCESSION   NZ_CP035206
VERSION     NZ_CP035206.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN10740431
            Assembly: GCF_009387775.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 6364)
  AUTHORS   Hadjadj,L. and Rolain,J.-M.
  TITLE     Genome of KpLH94
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6364)
  AUTHORS   Hadjadj,L. and Rolain,J.-M.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JAN-2019) MEPHI, IHU Mediteranee Infection, 19-21 Bd
            Jean Moulin, Marseille 13005, France, Metropolitan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. v3.10.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 21.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/26/2023 03:46:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,614
            CDSs (total)                      :: 5,493
            Genes (coding)                    :: 5,334
            CDSs (with protein)               :: 5,334
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 66 of 159
            Pseudo Genes (incomplete)         :: 98 of 159
            Pseudo Genes (internal stop)      :: 31 of 159
            Pseudo Genes (multiple problems)  :: 31 of 159
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP035206.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6364
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="LH94"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pLH94-2"
                     /geo_loc_name="Laos"
                     /lat_lon="19.52 N 102.10 E"
                     /collection_date="2012"
                     /collected_by="IHU"
     gene            join(6246..6364,1..>1)
                     /locus_tag="EQH49_RS28385"
                     /pseudo=""
     CDS             join(6246..6364,1..>1)
                     /locus_tag="EQH49_RS28385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /name="MbeD family mobilization/exclusion protein"
     gene            <2..325
                     /locus_tag="EQH49_RS29345"
                     /pseudo=""
     CDS             <2..325
                     /locus_tag="EQH49_RS29345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000635827.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease"
                     /name="nuclease"
     gene            495..1409
                     /gene="lpxO"
                     /locus_tag="EQH49_RS28395"
                     /old_locus_tag="EQH49_28735"
     CDS             495..1409
                     /gene="lpxO"
                     /locus_tag="EQH49_RS28395"
                     /old_locus_tag="EQH49_28735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001728425.1"
                     /GO_process="GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A hydroxylase LpxO"
                     /protein_id="WP_023343074.1"
                     /translation="MLSLIIIFVFLISVLYAHSRGKIKQKFSRQLFDHSTFMAPINMFM
                     TGFSKLPSKQAWFPPETFPELQPLIDNWQVIRDEAVNLQGHIKSSEKNNDAGFNTFFKR
                     GWKRFYLKWYGDAHPSAERLCPVTTQLVSSIPCIKAAMFAELPSGSHLGKHRDPYAGSV
                     RYHLGLSTPNDERCFIEVDGVRRSWHDGEVIIFDETFVHWAQNDTEQTRIILFCDIERP
                     MKWRWSQKINRWIGGVLMSAAASPNDNNDRTGAINHLFKYVNAIRDAGQKLKKRNRKLY
                     YLIKNGVISLIFVWLAVSVFRWL"
                     /name="lpxO"
     gene            2072..2608
                     /locus_tag="EQH49_RS28400"
                     /old_locus_tag="EQH49_28740"
     CDS             2072..2608
                     /locus_tag="EQH49_RS28400"
                     /old_locus_tag="EQH49_28740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005200081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_072159713.1"
                     /translation="MLDTDCITVLTARGITEILNSGGSQAWRLDATHVAKLPYLVCVQN
                     SKKDWGSQEAKHHHAFMIGKISGVSRAPENPKRWIINIDSYAEIDIPGQWDGNRNPVSY
                     RKLEDMGIDISKVDFQPLPKEDSLVLNTIKGGDEYDEYEEDSNRVRPINLKEAKAGLAL
                     YFGVNEDDIQITIKG"
                     /name="hypothetical protein"
     gene            2613..3203
                     /locus_tag="EQH49_RS28405"
                     /old_locus_tag="EQH49_28745"
     CDS             2613..3203
                     /locus_tag="EQH49_RS28405"
                     /old_locus_tag="EQH49_28745"
                     /inference="COORDINATES: protein motif:HMM:NF019843.3"
                     /GO_function="GO:0005515 - protein binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="WP_023343079.1"
                     /translation="MPSLSVERWLALATAGDSQARFHLGKIFSCGLGVHQDYKQAIYWF
                     QLAAEQGHDDAQFSLGWAYHLGLGGYKDAQKASEWYQKAAEQGHAEAQFQLGRLYAFSD
                     LRNDALAVEWFLCAAKQDHSSAQFKLGWMYHLGMGVEQSDRQAFHWFILAAEQDHAGAQ
                     ASLGEMYKNGWGVEQDDVAAQDWFYRARTMHYP"
                     /name="tetratricopeptide repeat protein"
     gene            3286..3747
                     /locus_tag="EQH49_RS28995"
     CDS             3286..3747
                     /locus_tag="EQH49_RS28995"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_065707752.1"
                     /translation="MVNTYYGYLLGVTLGEWAMGSDVQWQIDNGYLAQVMGDFSTESPS
                     FETPMFGRYFDTFQAAIHVAKAFPGSTLQPSEYEDKTGMLIRHYYVDCHPYYDRLPPEK
                     SDNFQYDEYPSAIHVAACIDQASKELDELLFGGGYGENGEGHASPIILY"
                     /name="hypothetical protein"
     gene            complement(4002..4112)
                     /locus_tag="EQH49_RS29230"
     CDS             complement(4002..4112)
                     /locus_tag="EQH49_RS29230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_048246078.1"
                     /translation="MHDPPVQSDYHAHSGNYQLESNPERHDKIAIGGSHW"
                     /name="hypothetical protein"
     gene            4756..5079
                     /locus_tag="EQH49_RS28425"
                     /old_locus_tag="EQH49_28765"
     CDS             4756..5079
                     /locus_tag="EQH49_RS28425"
                     /old_locus_tag="EQH49_28765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001445156.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQVVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            5288..>5722
                     /locus_tag="EQH49_RS29275"
                     /pseudo=""
     CDS             5288..>5722
                     /locus_tag="EQH49_RS29275"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            5757..6242
                     /locus_tag="EQH49_RS28435"
     CDS             5757..6242
                     /locus_tag="EQH49_RS28435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_023343076.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMLRMVSQTWLTIVLVSVLLIASNAAILWWQSQQIL
                     DNYVSIREQKSTQAMLSERNSGVQLSTCGEQRRRCVRVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     Typing          4469..4652
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="87.234"
                     /coverage="86.0"
                     /evalue="2.69e-52"
     Typing          complement(4003..4486)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.09"
                     /coverage="92.0"
                     /evalue="3.59e-159"
     Typing          5070..6364
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.75"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aatagcacat gacagagctg gaaaaacagt tgctgagcgc attagagcag ctacagcagg
       61 actactcgaa aaggctggac gagtgggaga gcgccttcgc ggaatggcgg acgatgtctg
      121 gtcttatgca acgggagaac gcggcgctga gcgagcgcgt cacgggcttg agcaggcagg
      181 tgcagagttt gagcgatcag ctgcgccggt tgtcgagcgg ctgaatgccg tcgagctgca
      241 gcaggtgcgg gaggcgcggc atcagaaagc gctggagctc gaacgatcac agcgacagcg
      301 ggtatatcac ggtccgtccc tttgattcat ttcagcataa ttcctgctga aatcctctct
      361 gcctgtgtag tagatatgtt ctggtgaagc ttcgattata ctattgataa atagcattta
      421 gattataatt taagtgcatt cattatgtta tctttaagag atttttatta ttggcgtgaa
      481 caaggaatat acacatgctg tcacttatta ttatttttgt ttttcttatc agtgtattgt
      541 atgcgcattc tcgcggtaaa ataaaacaaa agttttccag gcagttattt gatcattcaa
      601 catttatggc tccgataaat atgtttatga caggcttttc gaagctgccg tcaaagcagg
      661 catggtttcc cccagaaaca tttcctgaac ttcaaccatt aattgataac tggcaggtta
      721 taagagacga agctgttaat ttgcaagggc acattaaatc atctgaaaaa aataatgatg
      781 caggatttaa tacatttttt aagcgcggtt ggaagcgctt ttacctcaag tggtatggag
      841 atgctcatcc atcagcggag cggctttgcc cagtaacaac ccaactggtt agctctattc
      901 cctgtattaa agcagcgatg tttgctgaat tgccttcagg aagccattta ggaaagcatc
      961 gtgaccctta tgccggttca gttcgttatc atttaggatt atcaacacca aatgatgaac
     1021 ggtgttttat tgaagttgat ggtgtacgcc gcagttggca tgatggtgaa gtgataattt
     1081 ttgacgaaac ttttgttcac tgggctcaga atgataccga gcagacacgt attattttgt
     1141 tctgtgatat agaaaggccg atgaagtggc ggtggtcaca aaaaataaat aggtggatag
     1201 gtggggtgct aatgtcagcg gcagcatcac ccaacgataa taatgacagg actggtgcta
     1261 taaatcattt atttaaatat gttaatgcta ttcgtgatgc aggtcagaag ctaaaaaaaa
     1321 ggaatcgaaa actatattat ttgattaaaa atggagttat ttctttgatt tttgtttggc
     1381 ttgctgtctc agtgtttaga tggctgtgat ttgttttttt ctattgagtc atacataaat
     1441 ttgggtcttc tatgctcaat gtatggttat agataaaaat cgcctgctaa aaaattctat
     1501 tgaaatcttt ttagtaagaa aggcccgaat ctgacgctca gcgggccttt cgtgaagaag
     1561 tgcggcagta gacagatttc atctgatcac gttcatgtgc tgctgtgcaa ttaagcgttt
     1621 ctttcggagc tttcacggta tttccttcac gaaaccggcc gccattcact cttgtgcata
     1681 gccatgcagc cgcgcagaaa ttatcagggg cgtttttcag ggtttgggcc ggggaaaggt
     1741 gccggaaacg gccctcaggc cgcagaaacg cgctgagggg ctctcagggg gtgcggccaa
     1801 gaactcttat gttaaatgcg gcggtgtggc gggttcttac ttcacacatc atgttgtcac
     1861 tcagactgag tccagagaga aaaagccctc cggccgaagt cgagtagtgt tttactggtt
     1921 tttttgcagc cctaggagta atcgtcgtag tacttcggat agttatttgt atatatcttg
     1981 tggttttatg ttgagctctt ccgtttttga tgctaaaaag attgtcatta cgcggtgact
     2041 tgcctttttt aaaacaaaaa tggagatatc tatgcttgat actgactgca ttactgttct
     2101 tacggccagg ggtattactg agattttaaa tagtggagga agccaggctt ggagacttga
     2161 tgctactcac gtagctaagc ttccgtacct ggtttgcgtc caaaattcga aaaaagactg
     2221 gggtagtcag gaggctaagc atcatcatgc atttatgatc gggaaaatat ccggagtaag
     2281 tcgagcccct gaaaacccta agcgctggat cataaatatt gattcctatg ctgaaattga
     2341 tatccctggg cagtgggacg gcaatcgtaa tccagtctcc tatagaaaac ttgaagacat
     2401 gggaattgat atctccaaag tggatttcca acccctacct aaagaggatt ccttagtctt
     2461 gaatacgata aaaggcgggg atgaatatga tgagtatgag gaggatagca acagagttag
     2521 gccgataaat ctaaaagagg ctaaagcagg gttggcttta tattttggtg tgaatgagga
     2581 tgatattcag atcacaatta agggatgacc ttatgccgtc actctctgtt gagcgctggt
     2641 tagcactagc tactgctgga gatagccagg ctagatttca tctgggtaag atattctcat
     2701 gcggtcttgg tgtgcatcag gattataagc aggctatata ctggtttcag cttgcagctg
     2761 agcaaggcca tgatgatgcc cagtttagtc ttggttgggc atatcatctg gggttaggtg
     2821 gatataaaga tgctcaaaaa gcttcggaat ggtatcagaa agctgctgag caaggtcatg
     2881 cagaggctca gtttcagctt ggaaggctgt atgctttctc tgatttaaga aatgatgcgt
     2941 tagcagtcga atggtttctc tgtgctgcga agcaagacca cagtagtgcc caatttaaac
     3001 ttggctggat gtaccatctc ggtatgggcg ttgagcaaag tgataggcag gctttccatt
     3061 ggtttattct ggccgctgag caagatcatg caggagctca ggcatctctt ggtgagatgt
     3121 ataaaaatgg atggggggtc gagcaagatg atgttgctgc gcaggattgg ttttatcgtg
     3181 ccagaaccat gcactatccc taaacccgcc ctgtgcgggt ttttttacgc ctacgtgtaa
     3241 gaggcttgcg gagctggctg ctgctgattc ataacatgaa gcgttatggt aaatacatat
     3301 tatggttatt tattaggagt aactctggga gagtgggcta tgggatcaga tgttcaatgg
     3361 cagatagata atggttattt ggcgcaagtt atgggggatt tttcgacaga gtcgcctagc
     3421 tttgaaacgc caatgtttgg gcgatatttt gatacctttc aagctgctat acatgttgca
     3481 aaagctttcc cagggagtac gttgcagcca tcagagtacg aagataaaac tgggatgcta
     3541 atacgtcatt attatgtgga ttgtcatccg tattatgatc gactccctcc tgaaaaaagc
     3601 gataattttc agtatgatga gtacccttct gctattcatg tggctgcgtg tattgatcaa
     3661 gctagcaaag aactcgacga actattgttt ggtggtggat atggggaaaa tggtgagggg
     3721 catgcaagcc cgataatttt atattagtgt taggggcgga gggatattat ccaatttgta
     3781 aaatgatctt cttgagatcg ttttgctctg cgcgtaatct cttgctctga aaacgaaaaa
     3841 accacctggg gaggtggttt ttcgaaggtt cagtaagttg gggaacttct gaaccgtggt
     3901 aacagtcttg tgcgagacgt gtcaccaaat ctgtccttcc agtgtagccg tagtccggcc
     3961 accacttcaa gaactctcga tacatctctc gcacatcctg cttaccagtg gctaccgcca
     4021 atggcgattt tgtcgtgtct ttccgggttg gactcaagtt gatagttacc ggaatgggcg
     4081 tggtagtcgg actgaacggg ggggtcgtgc atacagtcca gcttggagcg aactgcctac
     4141 ccggaactga gtgtcaggcg tggaatgaga caaacgcggc cacataacag cggaatgaca
     4201 ccggtaaacc gaaaggcagg aacaggagag cgcacgaggg agctaccagg gggaaacgcc
     4261 tgggatcttt atagtcctgt cgggtttcgc cgccactgat ttgagcgtca tatttcgtga
     4321 tgcttgtcag gggggcggag cctatggaaa aacggcaacg ccgcatcctt ttcccagccc
     4381 ttcctgattt gagcgccatc tctttatccc gccattcgtg agccggtaac gcccgccgca
     4441 gtctcacggc aggagcgtag cgaccgagtg agcgaggagg cgtcatttca tcgtccccag
     4501 catttcgcac ttacgcccca cagccgcatt ttttacctgc cacatgaagc acttccctga
     4561 actcccactt cgatttacca tattaagcca gtatacactc cgctagcgct acgtgactgg
     4621 ttcagggctg cgccccgaaa cccgctaaaa ccactgacgc gcctgcggct tgtccaacac
     4681 cgcgccgacc gggaaaagat ttcccgcccg tcccggcgtt atcaggaggc cggattggca
     4741 gacaaacgca gcaaaatgct cacgatgtgg gtgaccgagg atgagcaccg gcgtctgctg
     4801 gagcgctgcg acggtaaaca gcttgcagcc tggatgcgcc agacgtgcct ggacgagaaa
     4861 cccgcccgcg ccggcaagct tccctcgatc tcgccggcgc tgcttcgtca gcttgccggc
     4921 atggggaaca accttaacca gattgcccgg caggttaacg ccggtggtgg cagcgggcac
     4981 gaccgcgtgc aggtcgtcgc cgcgctgatg gccatagatg ccggactcga gcggctgcgg
     5041 catgccgtgc tggaaaaggg tgcagacgat gatcgttaag tttcacccgc gagggcgcgg
     5101 cggtggcgcc ggtccggtgg attatctgct ggggaaagac cgacagcgcg agggcgccac
     5161 cgttctgcag gggaagccgg aagaagtcag ggagcttatc gacgcctcac cctacgcaaa
     5221 aaagtacaca tccggcgtcc tgtcctttgc cgaaaaggat ttaccgcccg ggcagcgcga
     5281 aaagctgatg gcgagctttg aacgggttct gatgcccgga ctcgacaaag accagtacag
     5341 cgtgctgtgg gttgagcacc aggataaggg gcggctggag ctgaacttcc tgatcccgaa
     5401 cacggagctg ctgactggca agcgtctcca gccgtattac gaccgcgccg accgtccgcg
     5461 cattgatgcc tggcagacca tcgtgaacgg caggctgggg ctgcatgacc cgaacgcgcc
     5521 tgaaaaccgg cgggcgctgg tcacgccgtc cgcgctgccg gaaacgaagc aggaagccgc
     5581 tcaggcaatt acacggggtt tactggcctt ggcctcgtcc ggggagctga aaacccgcca
     5641 ggacgtcact gaggcgctgg aaagcgcagg ttttgaggtg gtacgcacca caaaaagcag
     5701 catcagcatt gccgacccgg acggggggcg aaacatccga ctgaagggag ccatctatga
     5761 acagtctttt aacgctggcg aaggacttag agcagaaatc gaaagcgcag cagcagagta
     5821 ccggcgagat gctgaaagcc gcattcagcg agcacgagaa gtctgtcaga gcggaactga
     5881 gcgaaagcga gaagagaatc agcgccgcca tcctcgacca cgaccggaag ctgtcctcag
     5941 ccatgagcca gcgtacgaaa gggatgctgc gcatggtcag ccagacgtgg ctgaccatcg
     6001 tcctggtctc cgtgctgctg atcgcctcga acgcggccat tctgtggtgg cagagtcagc
     6061 agatactcga caattacgtg agcatccggg agcagaagag cacgcaggcg atgctgtccg
     6121 agcggaacag cggcgtgcag ctctcgacct gcggcgagca gagacgccgc tgcgtgaggg
     6181 tgaacccgga agcgggacgg ttcggagagg attcgagctg gatgatactg gcggggaaat
     6241 agcacatgac agagctggaa aaacagttgc tgagcgcatt agagcagcta cagcaggact
     6301 actcgaaaag gctggacgag tgggagagcg ccttcgcgga atggcggacg atgtctggtc
     6361 ttat
//
