LOCUS       NZ_CP035126             5167 bp    DNA     circular CON 18-JUN-2024
DEFINITION  Escherichia coli strain EC25 plasmid pEC25-3, complete sequence.
ACCESSION   NZ_CP035126
VERSION     NZ_CP035126.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN10743440
            Assembly: GCF_004088295.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5167)
  AUTHORS   He,F.
  TITLE     Carbapenem-resistant Escherichia coli strain harboring blaNDM-7 in
            China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5167)
  AUTHORS   He,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JAN-2019) Department of Clinical laboratory, Sir Run
            Run Shaw Hospital, School of Medicine, Zhejiang University, 3
            Qingchun East Road, Hangzhou, Zhejiang 310016, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Oxford Nanopore GridION; Illumina NextSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_004088295.1-RS_2024_06_18
            Annotation Date                   :: 06/18/2024 04:29:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,001
            CDSs (total)                      :: 4,881
            Genes (coding)                    :: 4,618
            CDSs (with protein)               :: 4,618
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 85 of 263
            Pseudo Genes (incomplete)         :: 176 of 263
            Pseudo Genes (internal stop)      :: 47 of 263
            Pseudo Genes (multiple problems)  :: 38 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP035126.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5167
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC25"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEC25-3"
                     /geo_loc_name="China: Hangzhou"
                     /collection_date="2017-08-17"
                     /collected_by="Fang He"
     gene            236..1150
                     /locus_tag="EQK40_RS25295"
                     /old_locus_tag="EQK40_25295"
     CDS             236..1150
                     /locus_tag="EQK40_RS25295"
                     /old_locus_tag="EQK40_25295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001271348.1"
                     /translation="MSAALQYFDENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWLVMEWREEAYTLDELADYLDLSASE
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLEAVIQRVEMYNASLPVPLSL
                     AECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGSKSKRSTVATSARTL
                     KPWEALGISRAWYYQLKKRGLVE"
                     /name="replication initiation protein"
     gene            complement(1354..1752)
                     /locus_tag="EQK40_RS25300"
                     /old_locus_tag="EQK40_25300"
     CDS             complement(1354..1752)
                     /locus_tag="EQK40_RS25300"
                     /old_locus_tag="EQK40_25300"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001222914.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLIDNEAGLTEQERK
                     LLHVLATAEKNDLQAQESERKQAVQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDRILQALKELKYDLETSPNPDA"
                     /name="hypothetical protein"
     gene            1929..2855
                     /locus_tag="EQK40_RS25305"
                     /old_locus_tag="EQK40_25305"
     CDS             1929..2855
                     /locus_tag="EQK40_RS25305"
                     /old_locus_tag="EQK40_25305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_001331297.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSGSLT
                     LTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAREHGFD
                     MRDRVSRQNRTAPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            2858..3391
                     /locus_tag="EQK40_RS25310"
                     /old_locus_tag="EQK40_25310"
     CDS             2858..3391
                     /locus_tag="EQK40_RS25310"
                     /old_locus_tag="EQK40_25310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000168770.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTILDRNYNATAAVYQKVVTLED
                     KLVEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            complement(3457..3666)
                     /locus_tag="EQK40_RS25315"
                     /old_locus_tag="EQK40_25315"
     CDS             complement(3457..3666)
                     /locus_tag="EQK40_RS25315"
                     /old_locus_tag="EQK40_25315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001096196.1"
                     /translation="MAKLGENTPHIIDATVDFMASSQAFREYLKKLPPDNTIPPALTDE
                     HVPLYIRRLEYYRLLYRPKQTESY"
                     /name="hypothetical protein"
     Typing          237..1011
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.778"
                     /coverage="83.0"
                     /evalue="0.0"
     Typing          1930..2852
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.052"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttgtcttttt gcgttttttg cgccagaaag cgactgaggg cgttttaagg ggggcgtaca
       61 acgggagtta tggtaaatgg atcgggtttt cgggaaggtt cgacaggatt tgccgttggg
      121 tgtagtgtaa gcgactgaaa aacaaacgcc cctgaaatca tgtccagatc cggcaagatt
      181 caacatgaaa tacagagggc gtgtaggtcg cataacccgg agagaatcgt aacacatgag
      241 cgccgcgctt caatacttcg acgaaaattt accccatcgt ccctatcaca cggatgatct
      301 cgcttttggt cttcgcatct ccggcaaagg gcgtgcgctt cttgcgcggt acatacagca
      361 gaaccagcct catgcgcagt tctggctggt ttttgacgtt gaccgcgagg gggctgcgat
      421 tgactggagc gaccggaacg ctcccgcgcc gaacatcacc gttaaaaatc ctgtgaacgg
      481 acacgctcac ctgctctacg cactcaacat cgccgtgaga accgcgcctg atgcctcggt
      541 taaggcgctg aaatatgccg cagcggttga acgttcgctg tgtgagaaac tttgtgcgga
      601 tgtgaactac agcggcctga tttgcaaaaa tccgttccac ctggaatggc tggtgatgga
      661 gtggcgcgag gaagcctata ccctcgatga actggctgat tatctcgatt tgagcgcctc
      721 agagcgtcgt agcatcgata aacattacgg gatggggcga aactgccacc tgttcgaaat
      781 gacgcgcaaa tgggcttaca gggcgattcg tcagggctgg ccagcgttct cacagtggct
      841 tgaagcggtg atccagcgtg tcgaaatgta caatgcatcg cttcccgttc cgctttcact
      901 ggctgaatgt cgggctattg gcaagagcat tgcgaaatac acgcacagga acttcacgcc
      961 ggaaactttc gcacagtatg tggctgatac gcacacgcca gaaattcagg ctgcacgcgg
     1021 tcgcaagggc ggttctaagt ctaagcgcag cacagtagct acatctgcac gcacgctgaa
     1081 accgtgggag gcattaggga tcagtcgcgc ctggtattac caactgaaaa aacgaggtct
     1141 cgtagagtag accaaataag cctatatcag ataacagcgc ctttctggcg tctttttgag
     1201 cagcaggttt tttgccgttt ttccccgttc agccctgcaa tggcgcggct ttccgcgtga
     1261 ttgtggtggt agtgctcgcc gcagtctcat gaccgagcgt agcgagcgaa tgagcgagga
     1321 agcaccaggg aatcggaaca cggatggagg cacttacgcg tctgggtttg gggatgtctc
     1381 cagatcgtac ttaagttcct taagtgcttg taatatcctg tcttttttat agcatagctc
     1441 gcctgttttt gtgtctacca gtcctgcttc gatcagtagc ccagccgcga gaaagagccg
     1501 gtgattgcgt tcccgtcgtt gtttttttcc ctcgattcgt ttttggaccg cctgttttcg
     1561 ttcgctctcc tgtgcctgta agtcgttttt ttccgctgtt gccagtacgt ggagaagttt
     1621 gcgttcctgt tctgtgagtc cggcttcgtt gtcgatcagg tcaataatct gtctctgtcg
     1681 tgcggtctgt tctccgagct tcctgatgaa tccgatccgt ttcttcagcc attcgatgtt
     1741 gctgatgcgc atgttcgtga tgctccctgg tgtttttttg cggtgtgttg tggtcgtgca
     1801 gtatatcgct tgcggtcggc gggtgcaggg ggtatcttcc gtgctaattc gtaaaattag
     1861 aaggaagcgc acttatatgt ttctctcctt cgtcgagaaa cagtgcgctc ctgcagaggg
     1921 ctgaaaccgt ggcaacctat cacctcagcg ttaaatttgg tggcaagggg caggctgcaa
     1981 accacgccga ttacatcgag cgcaaagaga aataccgcga ccgtcaggat ttggaatatt
     2041 ccgcacacgg aaacatgccg gaatgggcca gggataatcc ttcccatttc tggcaggcgg
     2101 ctgaccagtt tgaacgtgcg aacggttcga cctataggga acttgaaatt gcattacccc
     2161 gtgaactgac accggaacag cgccttgaac tggtgcagga ttttgtacgt caggaagctg
     2221 gcgaacggca cgcctggagt tttgccattc acaatccgaa agcgagcatt gacggcggcg
     2281 aacagcctca tgcgcatatc atgatgagtc agcgtgtgaa tgatggcatt gaacgtacac
     2341 cggaacagta tttccggcgc tataacgcca ggtatcccga gcggggaggt gcgaaaaagg
     2401 attccggaag cctgactctc actcagcaaa aagagcagtt gcgggaattg cgaaaacgct
     2461 gggaggtgaa gcacaacgaa catatgagaa agcatggctt tgaaaggggc ttcattgact
     2521 gccggacgct gaaagaacag ggcattgaac gacgtcccga agtgcatctg ggttttgaag
     2581 ctgccggacg gcttgatgat gcccagcgtc atgacatcat gcaaaaacgg tctgaaccgg
     2641 actattcccg ccatcttgaa caggcgcgaa cggtcgggca tgacatcagg gaatggctgg
     2701 acactccggt taagcaacat gaactggtca gaaggctgca acatgatgtt gcgcgggagc
     2761 atggctttga catgagagac agagtgagca gacagaaccg aactgcacct gaacgggtta
     2821 aatcaccgtc acgtggattt tctatgggat tctgaacatg gccagcacag aagaaaaatt
     2881 actcacgctc ctgttatcgg tggagcatct gcaacatacc gcaatggagc aacagaaagc
     2941 tgttgctgat tctctggctg tggcaaggga aactcttcgc ggatatcggg atatttctga
     3001 cggtgtggca aaacaggtca gggatgaagt cagaaatgag gtgcagaagc tggacgtggc
     3061 aggactgata ggtgagcgca tcagccagga ttttcagcgg ctggaagagt cagtcgcaaa
     3121 catgaaggca aacgcggatg ccctgaacag ggatattgcg acaattcaca gcaatatcgt
     3181 gcaggaatac aacaccctta aagggtatag ctggaaatgg ctgggtgggg tgtcagtggc
     3241 ttttctggtt atgttctgtg tgctcggatg gggaatgaaa acaatactcg acaggaacta
     3301 caacgccaca gcggcggtgt atcagaaagt tgtgacactg gaagataaac tggtggagaa
     3361 acctgtcacg gggaagagcc ggaaaaactg acgcgcttcg cttgtccaac gcctcacgac
     3421 ccgcgaaagg atttcgcgaa ccgttcaccg tttttttcaa tatgattctg tctgctttgg
     3481 tcgataaagc agacgatagt attccagcct tcgtatatat aacggtacgt gctcatcagt
     3541 aagagctggc ggtattgtgt tatcaggggg aagttttttc agatactcac gaaatgcctg
     3601 gcttgatgcc ataaaatcta cagtagcatc gataatatgt ggggtgtttt cacctaattt
     3661 tgccatgatg ataatcctta tattatcacg gtttgtgaat gatagggtgt cactattatt
     3721 tatgcgttag tgaaccagaa aaaatccccc gttggcatag ggggatatgt aaggagaaat
     3781 aacttcgcag gtgttcttgt ttgtataacg tgaaaagact gcacgcaact taataagtct
     3841 gccgtactta ctttcagttt tcaacataag gtttataaca atgtatggaa tggaagtgag
     3901 ctttaaggtt tcagctctaa aaaataaaat atttattagt tggctggttg ttgttcttgt
     3961 tctgtatgta tataaacact atcgacatat tatgaccggg aacattcctt accccctctg
     4021 cgctcgcaga ggtttttttt gtcattaatt aataatgaac tgaaatgcag ttccgatacc
     4081 tccatttgca cattatccct gacatatggt accggaaccg cattaatttt atgttataag
     4141 agtttatttt taatgtccag atggtttaat agtatttata tgtaattagc tgtatggata
     4201 gtactggttt tattgtataa cttgtcgttt ttttttattg tttttgtgtg gtgtgctaat
     4261 ctttaagtta taaaaaaacc ccttggaata caggggcaaa agtaaggaaa acaaatggat
     4321 gctcagtttg tgtgacactt taaactaatg gaactataga gtggaggttg ttacagtcgc
     4381 ttttataaaa cagcaaccag tttttttcaa tagtctgatg atggttgtga cagttttgtg
     4441 atattgttca aaatgtttct tgttgaatgt ttgttttttg gtctattggt gtctggtttt
     4501 attttttctg ctattacata tcaagaaatg tgattggtgt agcaagagtg gaatgcgatg
     4561 tggataaact aattggttgt cgaatctaat ttacttagaa aactacactt gtttttgtgt
     4621 atgaatttag tgaatttgtc tgtctatgtg ttttcgtgca tttataaatc cttcgtcagg
     4681 atcttaaatt tagtgttgaa tatgtagggc ctgtacggta attattattt agcgtagcta
     4741 cttcatcgtt gttcgaacac aaccaacaac ctgtaattgt tgtttctctt ggttccccac
     4801 ttttgtgggg ttttttatct cttccgtgca tcgtaaagcg cggactcctt ctggaaaggt
     4861 tgttttaggg catcactgag gcattttttg cgtttgtgaa tattgagcat ccgataacta
     4921 aaatatttcc catagactct gattcttgcc accgacccca ggtcggtttt tttttgtcct
     4981 ggcgagatgt gaagtttgga aacaggaaac cccgtcagag cgccacgcac caggggagaa
     5041 aacttgtcgt tttgagccgt tcggtgttca gaacgcacga aaccgatcgc acatcgcttt
     5101 cgtgcatagt tatgcaacgc catgaaaacg attctgacgc gtttttctgg tttggcctgg
     5161 tgttttc
//
