LOCUS       NZ_CP034765             2927 bp    DNA     circular CON 05-JAN-2024
DEFINITION  Klebsiella pneumoniae strain NB5306 plasmid unnamed6, complete
            sequence.
ACCESSION   NZ_CP034765
VERSION     NZ_CP034765.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN10662038
            Assembly: GCF_004006035.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 2927)
  AUTHORS   Weng,X.-B.
  TITLE     OXA-232 Carbapenemase-Producing Klebsiella pneumonia (ST437) from
            bloodstream isolated in China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2927)
  AUTHORS   Weng,X.-B.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-DEC-2018) Department of Medical Laboratory, Ningbo
            First Hospital, No.59, Liuting Street, Ningbo 315010, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: ABySS v. Jun-2018
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 20.0x
            Sequencing Technology             :: Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/04/2024 22:27:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,308
            CDSs (total)                      :: 5,185
            Genes (coding)                    :: 5,101
            CDSs (with protein)               :: 5,101
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 32 of 84
            Pseudo Genes (incomplete)         :: 50 of 84
            Pseudo Genes (internal stop)      :: 9 of 84
            Pseudo Genes (multiple problems)  :: 7 of 84
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP034765.1.
            Bacteria and source DNA available from Xing-bei Weng.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2927
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="NB5306"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed6"
                     /geo_loc_name="China: Ningbo"
                     /lat_lon="30 N 120 E"
                     /collection_date="2014-09-24"
                     /collected_by="Xing-bei Weng"
     gene            128..271
                     /locus_tag="EMI85_RS28740"
                     /old_locus_tag="EMI85_27045"
     CDS             128..271
                     /locus_tag="EMI85_RS28740"
                     /old_locus_tag="EMI85_27045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_058671098.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHDLSPLAAATGNGFAEQRSV
                     EV"
                     /name="hypothetical protein"
     gene            561..938
                     /locus_tag="EMI85_RS27050"
                     /old_locus_tag="EMI85_27050"
     CDS             561..938
                     /locus_tag="EMI85_RS27050"
                     /old_locus_tag="EMI85_27050"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_110982627.1"
                     /translation="MIHNHPTWVIKSMIYRLGILSISLLTVGCVSTSNVLELGRDTYTV
                     SATADGFRDAASARQSAFKTGSEKCNSLGKKFLLTNENVANTRMGIDTTVTLYFRCLDE
                     RDSEYVRPNLQKAPDLRIESK"
                     /name="hypothetical protein"
     gene            953..1300
                     /locus_tag="EMI85_RS27055"
                     /old_locus_tag="EMI85_27055"
     CDS             953..1300
                     /locus_tag="EMI85_RS27055"
                     /old_locus_tag="EMI85_27055"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_110958373.1"
                     /translation="MIMILWWISSFITHMMKVGNVREYGPTKSHLLLMVTTLPAGVGVF
                     IYPIVEVIWGDLSLLKVAGYTFLSLIVGGLLANIIVRGLSATTFGILLVNPLAMICSIA
                     GLIVLAIQHLP"
                     /name="hypothetical protein"
     gene            complement(2026..2448)
                     /locus_tag="EMI85_RS27065"
                     /old_locus_tag="EMI85_27065"
     CDS             complement(2026..2448)
                     /locus_tag="EMI85_RS27065"
                     /old_locus_tag="EMI85_27065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_127713065.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKDIKVID
                     VSELIRVFGSLDGVTDTVKYDVKSDAKSTGINSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKHEPSSPSHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(2448..2639)
                     /locus_tag="EMI85_RS27070"
                     /old_locus_tag="EMI85_27070"
     CDS             complement(2448..2639)
                     /locus_tag="EMI85_RS27070"
                     /old_locus_tag="EMI85_27070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_119719521.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEMLNELDLDAEADLCEKLHDDA
                     EHLFRTLSSRLDSLQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          8..418
                     /name="rep_cluster_2374"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="87.5"
                     /coverage="98.0"
                     /evalue="6.51e-131"
     Typing          2761..2922
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="87.117"
                     /coverage="98.0"
                     /evalue="3.29e-44"
ORIGIN
        1 tccctcgtgc gctctcctgt tcctgccttt cggtttaccg gtgtcactcc gctgttatgg
       61 ccgcgtttat ctcattccac gcctgacact cagttccggg taggcagttc gctccaagct
      121 ggactgtatg cacgaacccc ccgttcagtc cgactgctgc gccttatccg gtaactatcg
      181 tcttgagtcc aatccggaaa gacacgactt atcgccactg gcagcagcca ctggtaacgg
      241 attcgcagaa caaagaagtg ttgaagtgtg aggtgaagta cagctacact gtaataacta
      301 atttggtttc tgtgctctgc gaaagccagt tacctcggtt caaagagttg ctgctctaag
      361 aaccttcgaa aaaaccgcct tgctgggcgg ttttttcgtt tccaggccaa gagattacga
      421 cgcaacagaa aggatctcaa gaggatccta ttccatccct ctccccccca aagggaaacc
      481 agcaccactc cacgacacca gttctttgaa gaaaattatt gaaccccttg attttaataa
      541 taaaaaaaac agatataatc atgatccaca atcacccaac ttgggtaata aaatcaatga
      601 tttataggtt aggtatttta tcaatatcgc tgcttacagt tggttgtgtt tctaccagca
      661 acgttcttga gttaggtagg gatacatata cggtttcagc aactgctgat ggttttagag
      721 atgcggcatc agcacgtcaa agtgccttta aaacaggttc tgagaaatgt aatagcctcg
      781 gtaaaaaatt cctgctcact aacgaaaacg tggctaacac aagaatgggg atagatacca
      841 cagtaacact ctacttccgt tgtcttgacg aaagagacag cgagtatgta cgaccaaatc
      901 tacagaaagc acctgatcta cgcatcgaga gtaaataatt atgaatttcc ttatgatcat
      961 gatcctatgg tggatatcgt cattcatcac acacatgatg aaagttggaa atgtcagaga
     1021 gtacggtccc actaaaagcc atttactgtt gatggttact acgcttcctg ctggcgttgg
     1081 tgtttttatc tatccgatag tagaggtgat atggggagat ctgtcgttac tgaaagttgc
     1141 tggttatacc tttttatccc tcattgttgg cggacttctt gccaatatca ttgttcgcgg
     1201 attatctgcg acaacattcg gcattttgct cgtaaatcca ttagcaatga tatgtagcat
     1261 tgccggactg atagtccttg ctatccaaca tttaccataa catccgttat cagcaccgcc
     1321 tgagaggccc tcagggcgtt tctgacggga aaccggtaaa acaggcaaca aaccacccga
     1381 aaacccgcca gaaacgcgca ggctctgttt ttacggcatg catgagtatg cgcgcaagtg
     1441 catagcgcgg aaattcacac cattcagcca ggggaagtcc tgtttccggc gttcttctcc
     1501 tccgaaaacg gtgaacgggg acgcaattta ttgcgtggcc gtgaagcgtt gccggcgcag
     1561 ccggaaaacc ggttttcagc accttcagga atcgggttcg tcgacggccg gcaatcacgt
     1621 tatggcaagt cgccggacga ctcaccacac gattcaccgg cgattcgaat cgtgaattga
     1681 atcaagagtc accatgagca aaaaacaccc aaagtgatga attgaatcaa gagtcgattc
     1741 atcataacaa tatgataata aagcgtgatt cacatatgaa tcgtgggcaa ttcgtcagcg
     1801 attcgccata gagtcgtcca cgacttggat ctgcactttg taacacatca ccgcacattt
     1861 atttttcatt aacccattgt tgtaaataat gaatctgcaa aattttaatt ttgcgtgggg
     1921 tgtgggtatt tttagtggtg aggagcgttc agagatctgg cgatttttgg gaaggttcag
     1981 aaactctccg ggatggccgg agaggaaaac tcaagcatgg caggatcagg attttttcca
     2041 gaatttccac cacggggaat gggatggtga agaaggttcg tgcttgtgct ccagaagctg
     2101 catagcctga cgaagggaat caatatgctc atctcgagcc ttaacagccc ctttaagacc
     2161 atcgttttca gcttcaagta aagcgattct atgttgtaaa tcatgtaaac caacgctgtt
     2221 aatacctgta gattttgcgt cactttttac atcatatttt acagtatcag ttacaccatc
     2281 aagagaacca aacaccctaa taagctcgga tacatcaata actttaatat ccttcccgtg
     2341 ctttacaaca gaggtataac ttaacttccc gctgttaatc atcttgtaaa tagtgggacg
     2401 actaacctta acaagccgtg cagcttcact aatagacact ctggccatca gttaccatcc
     2461 tgtaatgaat ccagtctcga ggagagtgta cggaaaagat gctctgcatc atcgtggagt
     2521 ttctcacaaa ggtcggcctc agcatcaaga tcaagctcgt taagcatctc cagcaggagc
     2581 agagactgat tctggatgaa tttagccatg ttcagcgccg ttttctgttg tttcgtcata
     2641 aagggcctca gagcgtcagt gctcagtgta aaacctgtct cttttccgct tcctcgctca
     2701 ctcagtcgct acgctccggt cgttcggctg cggcgagcgg caggagcatg catagaaaag
     2761 ctcgagaaaa ccgagcggtc aaaggaggaa aaaaaggaaa ggccacggcg ttgccgtttt
     2821 tccataggct ccgcccccct gacaagcatc gcagaaacgg acgctcaaat cagtggtggc
     2881 gaaacccgac aggactataa agataccagg cgtttccccc tggcggc
//
