LOCUS       NZ_CP034764             3674 bp    DNA     circular CON 05-JAN-2024
DEFINITION  Klebsiella pneumoniae strain NB5306 plasmid unnamed5, complete
            sequence.
ACCESSION   NZ_CP034764
VERSION     NZ_CP034764.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN10662038
            Assembly: GCF_004006035.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3674)
  AUTHORS   Weng,X.-B.
  TITLE     OXA-232 Carbapenemase-Producing Klebsiella pneumonia (ST437) from
            bloodstream isolated in China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3674)
  AUTHORS   Weng,X.-B.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-DEC-2018) Department of Medical Laboratory, Ningbo
            First Hospital, No.59, Liuting Street, Ningbo 315010, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: ABySS v. Jun-2018
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 20.0x
            Sequencing Technology             :: Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/04/2024 22:27:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,308
            CDSs (total)                      :: 5,185
            Genes (coding)                    :: 5,101
            CDSs (with protein)               :: 5,101
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 32 of 84
            Pseudo Genes (incomplete)         :: 50 of 84
            Pseudo Genes (internal stop)      :: 9 of 84
            Pseudo Genes (multiple problems)  :: 7 of 84
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP034764.1.
            Bacteria and source DNA available from Xing-bei Weng.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3674
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="NB5306"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed5"
                     /geo_loc_name="China: Ningbo"
                     /lat_lon="30 N 120 E"
                     /collection_date="2014-09-24"
                     /collected_by="Xing-bei Weng"
     gene            complement(287..568)
                     /locus_tag="EMI85_RS27015"
                     /old_locus_tag="EMI85_27015"
     CDS             complement(287..568)
                     /locus_tag="EMI85_RS27015"
                     /old_locus_tag="EMI85_27015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_032440460.1"
                     /translation="MIKNIKHKGLRSFAEEGSTAGIQPSMAKRLRMILTTLSTATCIQD
                     VVDVRALGCHPLKGERKGEYSVEVNGNWRVVFKVEEPNVLDVDLEDYH"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            906..1199
                     /locus_tag="EMI85_RS27020"
                     /old_locus_tag="EMI85_27020"
     CDS             906..1199
                     /locus_tag="EMI85_RS27020"
                     /old_locus_tag="EMI85_27020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609279.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="WP_023317864.1"
                     /translation="MINLYDPVHPGEFIREMFMEPFEIPAAELADKLDVSQSTLSRLLN
                     GKTDLSYEMALRLSKVVGRSAESWVNMQTQFSLSKSRKSLAAELEHLHPMFP"
                     /name="HigA family addiction module antitoxin"
     gene            1263..1565
                     /locus_tag="EMI85_RS27025"
                     /old_locus_tag="EMI85_27025"
     CDS             1263..1565
                     /locus_tag="EMI85_RS27025"
                     /old_locus_tag="EMI85_27025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006326557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3892 domain-containing protein"
                     /protein_id="WP_023317863.1"
                     /translation="MVDFYISAIKMDSAGQHIEFVKVHKINRDGSWNTAGSISSRKFIS
                     ELISMGKASFMTVVKNSSTGKWNLGAKVEAMNDGYITTDPNKTKLDNLGNLPKFV"
                     /name="DUF3892 domain-containing protein"
     gene            complement(2349..2750)
                     /locus_tag="EMI85_RS27030"
                     /old_locus_tag="EMI85_27030"
     CDS             complement(2349..2750)
                     /locus_tag="EMI85_RS27030"
                     /old_locus_tag="EMI85_27030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_023317862.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKEGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSEQRDTASADQGEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(2750..2941)
                     /locus_tag="EMI85_RS27035"
                     /old_locus_tag="EMI85_27035"
     CDS             complement(2750..2941)
                     /locus_tag="EMI85_RS27035"
                     /old_locus_tag="EMI85_27035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_023317861.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGY"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3357..3503
                     /locus_tag="EMI85_RS28580"
     CDS             3357..3503
                     /locus_tag="EMI85_RS28580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001717510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_073545459.1"
                     /translation="MHEPPVQSDCCTLSGNYHLESNPVRHAQAPLAAATGKKVYKRLLI
                     QSS"
                     /name="replication initiation protein"
     Typing          3063..3466
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.309"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 agtcaaggaa gaaccaagac agctcactca ccagcaggct cctttttatt ggtagacaca
       61 tgcttctgga cagtatcagc ccctgcacaa agtacagcag ctaacaagaa ccatccccag
      121 ccatcaatac cacgaaaagc taggactcca gcaatagtta cgctaatcaa cgggaacaat
      181 aaaaccataa aggatctcat actttctcca gaggttactt ttttaataaa gtccctaatc
      241 catccgtact ttcacttgga tggattagca acttacttat tgataactaa tgataatctt
      301 caagatcaac gtctaagacg tttggttcct caaccttgaa aactacccgc cagttcccat
      361 taacttcaac ggaatactcg cctttacgct ccccctttag ggggtggcac cctaacgccc
      421 taacgtcaac aacgtcttga atgcaagttg ctgtgcttag cgtagtcaaa atcatgcgaa
      481 gtcttttcgc catactgggc tgaataccgg cggtgctacc ttcttcagca aagctcctaa
      541 gtcctttatg ttttatgttt tttatcatct acaatcccca tatttttttc gtctatacct
      601 gtatctatat tttaaaatca ttttaattag ttccattttg cccccttgct ttattaaaac
      661 aattgacgta agtttgttct tgaggtcaat cacctcattt gacttcaacg aatcctaagg
      721 gtaaaatact gcgccttgaa aaggccataa aagcccatac atacgcagta gctttgactc
      781 caacgagtat cctaaagctg actgcttttt ttgtcaatat catgaacttg cacattgtgc
      841 aatgcgcata ctgaaaggca gtaatttttt ttgtataatc aaatacaaaa caaacagagg
      901 aaggcatgat aaatctttac gatcctgttc atcctggtga gtttatcaga gaaatgttca
      961 tggaaccgtt cgaaatacct gccgcagagt tagccgataa acttgatgta tcccagtcta
     1021 ccttaagtag actgttaaat gggaaaacag acttaagcta tgaaatggca cttagattgt
     1081 cgaaggtcgt aggccgctct gcagagtcgt gggtaaacat gcagacccaa ttcagcctgt
     1141 caaaaagtcg caaatcttta gcggcagagc tggaacactt gcatccaatg tttccatgaa
     1201 aaaagcccgc agaacgcggg cttttgttac gtaaaacacc cagcagccat caggaggcaa
     1261 ccatggtaga tttctatatc tcagcaatca aaatggacag tgctggtcag cacatcgaat
     1321 tcgtaaaagt tcataaaata aaccgagatg gctcatggaa cactgccggc tcaatcagct
     1381 ctcgcaaatt catcagcgag ctgatcagca tgggaaaagc cagctttatg acagttgtaa
     1441 agaacagctc tactggtaag tggaacctag gagccaaagt ggaagcgatg aacgatggct
     1501 acataaccac agatccaaac aaaactaaac tcgataactt gggaaatctg ccgaaatttg
     1561 tctgatatgc aataacatcc actaagttcc agcagaatcc cctccctaat gatttaacat
     1621 aaagtcataa ttgtacgcac cgctaaaacg gcctcatggc gtttctgacg ggaaaccggt
     1681 aaaacaggca acaaaccacc cgaaaacccg ccagaaacgc gcaggctctg tttttacggc
     1741 atgcatgagt atgcacgcaa gtgcatagcg cgaaaattca cgccattcag ccagggtaag
     1801 tcctgtttcc ggcgttcttt ccatccgaaa acggtgaacg gggacgcaat ttattgcgtg
     1861 gtcgtgagcc gttgccggcg cagccggaaa acccgttttc agcacgttca ggaatcgggt
     1921 tcgtcgacgg ccggcaatca cgttatggca agtcgccgga cgactcacca cacgattcac
     1981 cggcgattcg actcgtgaat tgaatcaaga gtcaccatga acaaaaaaca cccaaagtga
     2041 tgaattgaat caagagtcaa ttcatcataa ccatatgata ataaagcgtg attcacatat
     2101 gaatcgtggg caattcgtca gcgattcgcc atagagtcgc ccacgactcg gattcacctt
     2161 acaaaaaata aaattgcgta atttacaata gataactaat tgaaactaaa ggtctatctg
     2221 caaaatttta attttgcgtg gggtgtgggt atttttcgtg gtgaggagtg ctcagagatc
     2281 tggcgatttt tgggaaggtt caggaactcc ccggaatgac cggagaggaa aactgaagca
     2341 tgacgcggtc aggatttttt ccagaatttc caccatggcg ccggggattc agttaatttt
     2401 gcctggtgat tttcaagaag gagcatggcc tgtttcaggc tgtcaatgtg cgaatccttg
     2461 gacaaaagta aggtattcag gtgttcaatc tcctgtttca gatgacgaat cacctgttca
     2521 ccctggtcag cagaggctgt atcacgctgt tcagagacct gtccagaatg gtgttcaccc
     2581 ttcggttgct caaagggacc aaagacacgt aaaagctctg aagtatctac catttttgca
     2641 ttacgctcac cgctacaggt ggacaggtca ccttctttaa ttaacctgtg cagtgttgtt
     2701 ctactcttac ccgtcagtcg ggcagcctca ctaatagaca ctctggccat cagtaaccat
     2761 cctgtaatgc atccagtctc gaggacagtg tacggaaaag atgctccgca tcatcgtgga
     2821 gtttctcaca aaggtcggcc tcgacatcga gatcaagctc gttaagcttc tccagcagga
     2881 gcagagactg attctggatg aatttagcca tgttcagcgc tgttttctgt tgtttcgtca
     2941 taagaggcct cagagcgtca gtgctcagtg tagggatgtt ctcttttccg cttcctcgct
     3001 cactcagtcg ctatgctcct gccgttcggc tgcggcgagc ggcaggagca tgcgtgtaaa
     3061 agctcgagaa aaccgagcgg tcgcgtagcg gggaaaagga aaggccgcaa agcggccgtt
     3121 tttccatagg ctccgccccc ctgacaagca tcacagaaac ggacgctcaa atcagtggtg
     3181 gcgaaacccg acaggactat aaagatatca ggagtttccc cctgatggct ccctcgtgcg
     3241 ctctcctgtt cctgcctttc ggtttaccgg tgtcattccg ctgttatggc cgcgtttatc
     3301 tcattccacg ccagacactc agttccgggt aggcagttcg ctccaagctg gactgtatgc
     3361 acgaaccccc cgttcagtcc gactgctgca ccttatccgg taactatcat cttgagtcca
     3421 acccggtaag acacgcacaa gcgccactgg cagcagccac tggtaagaag gtgtacaaga
     3481 gattactgat acagagttct tgaagtctca gcccaactac ggctaaactg aaaggacaga
     3541 atttggtttc agcggtcttg tacaccctgt taccacggtt cagaagttcc ccaacttact
     3601 gaaccctcga aaaaaccgcc tccccaggcg gttttttcgt ctcggcgagt aagatacgct
     3661 ttatcattca aagc
//
