LOCUS       NZ_CP034763             4976 bp    DNA     circular CON 05-JAN-2024
DEFINITION  Klebsiella pneumoniae strain NB5306 plasmid unnamed4, complete
            sequence.
ACCESSION   NZ_CP034763
VERSION     NZ_CP034763.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN10662038
            Assembly: GCF_004006035.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4976)
  AUTHORS   Weng,X.-B.
  TITLE     OXA-232 Carbapenemase-Producing Klebsiella pneumonia (ST437) from
            bloodstream isolated in China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4976)
  AUTHORS   Weng,X.-B.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-DEC-2018) Department of Medical Laboratory, Ningbo
            First Hospital, No.59, Liuting Street, Ningbo 315010, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: ABySS v. Jun-2018
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 20.0x
            Sequencing Technology             :: Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/04/2024 22:27:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,308
            CDSs (total)                      :: 5,185
            Genes (coding)                    :: 5,101
            CDSs (with protein)               :: 5,101
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 32 of 84
            Pseudo Genes (incomplete)         :: 50 of 84
            Pseudo Genes (internal stop)      :: 9 of 84
            Pseudo Genes (multiple problems)  :: 7 of 84
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP034763.1.
            Bacteria and source DNA available from Xing-bei Weng.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4976
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="NB5306"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed4"
                     /geo_loc_name="China: Ningbo"
                     /lat_lon="30 N 120 E"
                     /collection_date="2014-09-24"
                     /collected_by="Xing-bei Weng"
     gene            join(4740..4976,1..666)
                     /locus_tag="EMI85_RS26985"
                     /old_locus_tag="EMI85_26985"
     CDS             join(4740..4976,1..666)
                     /locus_tag="EMI85_RS26985"
                     /old_locus_tag="EMI85_26985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016946055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_119719527.1"
                     /translation="MHNAALELFNERLPHKPYFSDDLHFGVRIAGKERAILAKYIQFNQ
                     PHAMFWLGFDVDRAGSAIDWSDRNAPAPTLTITNPENGHAHLLYALENSIRTAPDGKIK
                     PLRYAASVENALRKKLEADAGYSGLICKNPNHNHWKIAVWQPKLYTLDWLADFLDLNTG
                     NDKEIVADYGLGRNCTLFDKTRKWAYRAIRQGWPEYEQWLQACYERACAYNLQFARPLD
                     ENEVRGIAKSIAKWTSNKFSPEEFSKFVDITHSSEIQSKRGKKSGQSRRKGSLEEIKPW
                     VAMGISRRKYFYIKKKMVE"
                     /name="replication initiation protein"
     gene            668..1375
                     /locus_tag="EMI85_RS26990"
                     /old_locus_tag="EMI85_26990"
     CDS             668..1375
                     /locus_tag="EMI85_RS26990"
                     /old_locus_tag="EMI85_26990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004198042.1"
                     /translation="MKTFVTLWTIVFLAFIAVMCTVDISNYIPSVYTVFSKNRPLVTGA
                     LSILLISVFIWLMVSLYLLKKYSVKVEKLSLGGVNILFNESATLYKKSVKNHLDSKRAI
                     FKLRKNVDAFDEVISSYYQTYQFIRDEMKILNPKKDREIYDFSNEMLMVLNRFLTNYQN
                     NYKRWYKYISDKDEVVDRVSGDVMRFHATSINKIQMQYYDYERILKGFKDVNRFFVNDV
                     KPKFGINTDKWNW"
                     /name="hypothetical protein"
     gene            complement(1412..2026)
                     /locus_tag="EMI85_RS26995"
                     /old_locus_tag="EMI85_26995"
     CDS             complement(1412..2026)
                     /locus_tag="EMI85_RS26995"
                     /old_locus_tag="EMI85_26995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019725132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="WP_119719528.1"
                     /translation="MGKHKVFISYHHANDQYYKNAIEKMNEQHEIFVNRSVSLGDIDED
                     EAPQKIREIIRDKYLRDTSVLILLVGTETKNRKHVDWELYSSMRDSTINKKSGILVVNL
                     PSTNTTYIRSTHGTNEKSEVHPTVTEWFSIDKRSTYEERFPYMPARIIDNFMKSGAYIS
                     VVNWNQIENNPETLRKLIDMTYNDREKATYDMSREMRMHNS"
                     /name="TIR domain-containing protein"
     gene            2415..2816
                     /locus_tag="EMI85_RS27000"
                     /old_locus_tag="EMI85_27000"
     CDS             2415..2816
                     /locus_tag="EMI85_RS27000"
                     /old_locus_tag="EMI85_27000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_119719529.1"
                     /translation="MTRVSISEAARLTGKSRTTLHRLIKTGELSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEQHSEQVFEQRDTASADQNEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAAWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(3593..3754)
                     /locus_tag="EMI85_RS28215"
     CDS             complement(3593..3754)
                     /locus_tag="EMI85_RS28215"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_162870772.1"
                     /translation="MAKHWSGQECPKTGTYGQYHDTDNSYAGSEYDRHVSKGDKFPPSK
                     NNHHFQEK"
                     /name="hypothetical protein"
     gene            3991..4236
                     /locus_tag="EMI85_RS27005"
                     /old_locus_tag="EMI85_27005"
     CDS             3991..4236
                     /locus_tag="EMI85_RS27005"
                     /old_locus_tag="EMI85_27005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012848468.1"
                     /GO_component="GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0097351 - toxin sequestering activity [Evidence IEA]"
                     /GO_process="GO:0045927 - positive regulation of growth
                     [Evidence IEA]; GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="WP_025987695.1"
                     /translation="MHAINFTDARKHFAETMKRVTDDAEPVRVMRRDAPDIVMIDAGEY
                     EALIETVYLFSNPANAEHINESMKQAERGEFVEVEY"
                     /name="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
     gene            4217..4492
                     /locus_tag="EMI85_RS27010"
                     /old_locus_tag="EMI85_27010"
     CDS             4217..4492
                     /locus_tag="EMI85_RS27010"
                     /old_locus_tag="EMI85_27010"
                     /inference="COORDINATES: protein motif:HMM:TIGR02116.1"
                     /GO_process="GO:0008219 - cell death [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="WP_019725129.1"
                     /translation="MSKWNIKFTRNGADDLNYWRDTDKKKFERIRQLLLSIEADPLTGI
                     GKPERLRHHKDPALYSRRIDQSHRLVYSIQNGEIIIYAARYHYGDK"
                     /name="Txe/YoeB family addiction module toxin"
ORIGIN
        1 cccgaaaacg gacacgcgca cctgctgtac gcgctggaaa actcgatacg caccgcgccg
       61 gacggcaaaa taaaaccgtt acgctacgcc gcctccgtgg agaatgcgct acgtaaaaaa
      121 ttagaggctg acgctggata ttcggggctc atctgcaaaa atccgaacca caaccactgg
      181 aaaatcgccg tgtggcagcc gaagctctac actctggact ggctggccga ctttctcgat
      241 ctgaacacag ggaatgacaa ggaaatcgtt gccgactacg gcttaggccg taactgcacg
      301 ttgttcgaca aaacccgtaa gtgggcatat cgcgccatcc gtcagggctg gcctgagtat
      361 gagcagtggc tgcaagcctg ctacgaacgc gcttgcgcat acaacctgca atttgcacga
      421 cctctggatg aaaatgaggt aagagggata gctaagagca ttgctaaatg gacatcaaat
      481 aaattttcac ctgaagagtt cagtaaattt gttgatatta ctcattcctc tgaaattcaa
      541 tcaaagagag ggaaaaagtc aggacagtca aggaggaaag gaagcctaga ggaaataaaa
      601 ccttgggtag ctatgggaat aagcaggcgg aagtattttt atatcaagaa aaaaatggtg
      661 gaataagatg aaaaccttcg taactctttg gactatagtt tttctagcat ttattgccgt
      721 gatgtgtact gttgatatat ctaattacat tccgtctgtt tacacagttt ttagtaagaa
      781 tagacctctt gtaactggcg ccctttctat tcttttgata tctgttttta tttggctgat
      841 ggtatccctc tacttactta aaaagtattc ggttaaggtt gagaaactta gccttggtgg
      901 agtgaatata cttttcaatg agagtgctac actttacaaa aagagtgtaa aaaatcacct
      961 agattcaaaa agagcaattt tcaaattaag aaaaaacgtt gatgcctttg atgaggttat
     1021 ttcatcatat tatcagactt accaatttat tcgtgatgaa atgaaaatac taaaccctaa
     1081 aaaagatcgt gagatttatg atttcagcaa tgagatgctc atggtgttaa atagatttct
     1141 aactaactac cagaacaact acaagcgttg gtataagtac atatcagaca aggatgaggt
     1201 tgttgatagg gttagcggtg atgtcatgag atttcatgca accagcataa ataaaataca
     1261 aatgcagtat tatgattatg agcgtatttt aaaaggtttt aaagatgtta accggttctt
     1321 tgttaatgat gtgaagccta agtttggaat caatacagat aagtggaact ggtaaattaa
     1381 aggagtagcc aaggctactc cttggatagt actatgaatt atgcattctc atttctctcg
     1441 acatatcata cgttgctttt tctctatcgt tataagtcat gtctattaat tttctgagtg
     1501 tttctgggtt attttctatt tgattccagt tgaccactga tatatacgcc ccgctcttca
     1561 tgaagttgtc tattattctt gcaggcatat atgggaacct ttcctcataa gttgagcgtt
     1621 tatcaataga aaaccattca gttacggtag gatgaacttc tgatttttca ttagttccat
     1681 gtgttgaacg aatgtacgta gtatttgttg acgggagatt gacgactaaa attcctgatt
     1741 ttttgttaat ggtactatct ctcatgctag agtaaagttc ccaatctacg tgttttctgt
     1801 tttttgtttc agttccaaca agaaggataa gaactgatgt atctctaaga tatttatctc
     1861 ttattatctc tcttattttt tgtggagctt catcttcatc tatatcacct aaagatactg
     1921 agcgattcac gaatatttca tgttgctcat tcattttttc aattgcgttt ttgtagtact
     1981 gatcattcgc atggtggtag ctaatgaata ccttatgctt tcccattttt attgtccttt
     2041 ttttgtttgt gtgcactcta ttttgcactt gaacctatat cagataacag ctctcttaga
     2101 gagctgtttt tttcagggtc ccttcagcaa ggtctcacca tgactggttt agggagggag
     2161 ggctctttgc ttccctaaac gccttgagaa gagatctata acctcacttc attcgtacgt
     2221 accgctcgcc gcagtctcat gaccgaacgt agtgagcgaa tgcgcgagga agcggaaagt
     2281 catctgttgc ctgcattttg cacggtcact cctgtgtttc atcgcccttc cgaaaaacac
     2341 atcacttctg gaccggtatg tcgtaagctg gcccctgttt ccttgagact gaatgcatta
     2401 aaggatgata actgatgacc agagtgtcta ttagtgaggc tgcacggctg acgggtaaga
     2461 gccggacaac gctgcacagg ttaatcaaaa caggtgagct gtccacttgt agcggtgaac
     2521 gaaatgcaaa aatggtagat acttcagagc ttttacgtgt ttttggtccc tttgagcaac
     2581 ctaagggtga acagcattct gaacaggtat ttgaacagcg tgatacagct tctgctgacc
     2641 agaatgaaca agtgattcgt catctgaaac aggagattga acacctgaac accttacttt
     2701 tgtccaaaga ttcgcacatt gacagcttga aacaagccat gctccttctt gaaaatcacc
     2761 aggccaaatt aactgaatcc ccggcggcat ggtggaagtt ctggaaaaaa tcctgatccc
     2821 gcgatgcttc agttttcctc tctggccatc ccggggcgtt cctgaacctt cccagatacc
     2881 gccagatctc tgaacgctcc tcaccactaa aaatacccac accccacgca aaattaaaat
     2941 tttgcagata actcattaat atcagttagt tggttgttat taaattacgc agtattacct
     3001 tctataaggt caatccaagt cgtcggcgat tcgtgtacga cttgccgacg actcgtcata
     3061 gagtcatcga cgactcactc caaactatca gtaacttatg acgacttgac tctagagtcg
     3121 aatcgtcgct ttggcgtttt tttgaccgcg gtgactctag agtcaagtca tgagtcgaat
     3181 cgccggtgaa tcgtgtggtg agtcgtccgg cgacttgcca taacgtgatt gccggccgtc
     3241 gacgaaccgg ttttccggct gcgccggcaa cgcttcacgg ccacgcaata aattgcgtcc
     3301 ccgttcaccg ttttctgagg agaagaacgc cggaaacagg acttcccctg gctgaatggt
     3361 gtcaatttcc gcgctatgca cttgtgcgca tactcatgca tgccgtaaaa acagagcctg
     3421 tgcgtttctg gcgggttttc gggtggtttg ttgcctgttt tgccggtttc ccgtcagaaa
     3481 cgctctgagg gcctctgagg cggtgcgggc aacggatgtt atggtaaatc aagtgaatgg
     3541 caggtacaaa aaaagcccca gagggctttt agtttagact tctgaagata ttttattttt
     3601 cttggaagtg gtggttgttt ttgctcggag ggaacttatc acccttgctc acatggcgat
     3661 catattcaga accagcgtat gagttgtcgg tatcatgata ttgaccgtaa gtacctgttt
     3721 ttggacattc ttgcccagac caatgttttg ccatttactt ttcctttgct cgttttagga
     3781 tctaagctcc tagcggtacg aaattgtgcc gcacttcata aatgtaattg cttgaaatta
     3841 tgcttcaatg tattgacttg ttgtttttgt ggttattcga ttgagttcac attttgagct
     3901 gatttggttg tgggttgtta gggaaatatc tatatgtact tatttgtgta catgttctat
     3961 gctaagcccc aacaaatggg ggttaacaac atgcatgcaa tcaattttac tgatgcccgt
     4021 aaacattttg ccgagacgat gaagcgtgtc actgatgatg ctgaaccggt acgtgtcatg
     4081 cgccgtgatg cgcctgatat tgttatgatc gatgcgggag agtatgaggc gctaatcgaa
     4141 actgtctatc tgttcagcaa tccggcgaac gcagagcata tcaatgagtc aatgaaacag
     4201 gcggagcgcg gcgaatttgt cgaagtggaa tattaagttc actaggaatg gcgctgatga
     4261 ccttaactat tggcgtgata ctgacaaaaa gaagtttgag cgtatccggc aactgttgct
     4321 cagcatcgag gctgatccgc tgacaggtat aggaaaaccc gaacggctgc ggcaccacaa
     4381 agatccggca ctctactccc gcaggattga tcagagccac cgtctggttt attcaatcca
     4441 gaacggagaa atcatcatct acgcagcccg ttatcactat ggtgacaaat agcaaaagtc
     4501 gatatagccc gcttgatgcg ggcttttcat gcaaggcgta caacggatgt tagggtaagt
     4561 cgctgtcaga tcgacacaaa ggcaaagtcg gtgtaacgtt agcctcagaa aaaacaaagc
     4621 cccggaaatc atgtcatatc ttggcggatc gcatgataac cagggctagg tcgaaacctt
     4681 gaaaggatat tagcacatga accgtgccag acaacagccc gccataagcg cagggcacat
     4741 tgcataacgc cgcactcgaa cttttcaacg aacgcttacc acacaagccg tacttttcgg
     4801 acgatctgca ctttggtgtc cgtatcgccg gtaaggaacg cgctattctg gcgaaatata
     4861 tccagttcaa tcagcctcac gccatgttct ggctgggatt tgacgttgac cgtgccggtt
     4921 ccgccatcga ctggagcgac cgcaacgcgc ctgcaccgac gctgaccata actaac
//
