LOCUS       NZ_CP034741             1183 bp    DNA     circular CON 18-JUN-2024
DEFINITION  Escherichia coli strain L65 plasmid pL65-5, complete sequence.
ACCESSION   NZ_CP034741
VERSION     NZ_CP034741.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN10623993
            Assembly: GCF_003999795.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Liu,Z., Li,Y., Li,R., Xiao,X. and Wang,Z.
  TITLE     Emergence of NDM-5 prouducing Escherichia coli from a goose farm in
            Jiangsu, China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1183)
  AUTHORS   Liu,Z., Li,Y., Li,R., Xiao,X. and Wang,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2018) Veterinary, Yangzhou University, 88
            University South Road, Yangzhou, Jiangsu 225009, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. 4.4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003999795.1-RS_2024_06_18
            Annotation Date                   :: 06/18/2024 04:15:22
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,857
            CDSs (total)                      :: 4,739
            Genes (coding)                    :: 4,575
            CDSs (with protein)               :: 4,575
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 164
            CDSs (without protein)            :: 164
            Pseudo Genes (ambiguous residues) :: 0 of 164
            Pseudo Genes (frameshifted)       :: 73 of 164
            Pseudo Genes (incomplete)         :: 88 of 164
            Pseudo Genes (internal stop)      :: 39 of 164
            Pseudo Genes (multiple problems)  :: 30 of 164
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP034741.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1183
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="L65"
                     /db_xref="taxon:562"
                     /plasmid="pL65-5"
                     /geo_loc_name="China"
                     /lat_lon="32.40 N 119.40 E"
                     /collection_date="2018-05-28"
                     /collected_by="Ziyi Liu"
     gene            complement(join(1139..1183,1..498))
                     /locus_tag="EM854_RS24720"
     CDS             complement(join(1139..1183,1..498))
                     /locus_tag="EM854_RS24720"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="WP_205716857.1"
                     /translation="MIQVKFFTAEIKAKFASRGELAHQAQQAYHRALVQIYGDNLQIIK
                     GYYSERQECAMTFNEGHPPSKTDRTTVWKLEEKQTDVRMALEMYRDAIRGEAEQLVLCT
                     NDSDMIPPIEMIKADRPELKIGIVVPREQDTSRVISSLASLGDWSLPYVSYEALRLALL
                     PDKSLRWMRQSWVSTSG"
                     /name="NYN domain-containing protein"
ORIGIN
        1 tttatctggc aataaagcta atcttaaggc ttcataggat acatatggca agctccaatc
       61 ccctaaacta gccagactac taatcactcg actagtatct tgttcccttg gcactacaat
      121 tcctattttt aactcaggtc gatcggcttt tatcatttct attggcggta tcatatctga
      181 gtcgttagta cagagaacca attgttctgc ctcaccacgg atcgcatcgc gatacatttc
      241 taaagccatt ctaacgtcgg tctgcttttc ttctaacttc cagaccgttg ttctatccgt
      301 ttttgatggc ggatgcccct cattaaaggt cattgcacat tcctgacgct ctgagtaata
      361 acctttgatt atctgtaggt tatcgccata gatctgtact aatgctcgat ggtaggcttg
      421 ctgagcctgg tgcgccaatt caccccgaga agcaaacttt gctttgatct cagcagtaaa
      481 gaacttcacc tgaatcaact gtgactctct tgatcttatc ggaagtagtt gttcttcaaa
      541 gagtttatag agatctaacc acttatatcc cgggacagct taaccaatcc gtgataaaaa
      601 ttaaacccat ctacgtaaac tattgttctc atttttattt cgcgcataaa aaccgcctct
      661 aaaggcggtt ttaacaacaa aaactaacgt tagcaaacag tttaagttgt agttcctcag
      721 ggttaaaact aaccgagagg gcggtttcaa gctcctgaag gaagacgcgt aactaatcgc
      781 tgcccaccta aaagggaggt ttagagcctc aaaagggagc tgcgtaactt aatcgcgcta
      841 ccaaagaggt gagtgtagat taagctatag ttaaatataa tgatattata agcgcgatgg
      901 tgtcgattac taattatatg aattaattta ccaacctgca agctgaagtc ttctatgaag
      961 acttcagctt aacagattta tatggaatac aaaataattt agaccttcaa aacagacact
     1021 tgaatctaaa tgggggaagg aggcgttaaa gcatgggatg gtcagccatt cctacatccc
     1081 ttcttcctgc agggcgcact caatatgaca caacattcaa tgtgctactt catcctaatc
     1141 atccactggt ggaaacccat gattggcgca tccatctcaa aga
//
