LOCUS       NZ_CP034682            13478 bp    DNA     circular CON 26-NOV-2023
DEFINITION  Klebsiella quasipneumoniae strain D120-1 plasmid pD120-1_13kb,
            complete sequence.
ACCESSION   NZ_CP034682
VERSION     NZ_CP034682.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN10622699
            Assembly: GCF_003990375.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella quasipneumoniae
  ORGANISM  Klebsiella quasipneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 13478)
  AUTHORS   Yang,X., Bai,L. and Li,R.
  TITLE     Emergence of mcr-8.2-bearing Klebsiella quasipneumoniae of animal
            origin
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13478)
  AUTHORS   Yang,X., Bai,L. and Li,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2018) College of Veterinary Medicine (Institute of
            Comparative Medicine), Yangzhou University, Wenhui Road, Yangzhou,
            Jiangsu 225009, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: NOV-2018
            Assembly Method                   :: Unicycler v. 0.4.4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/26/2023 02:49:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,478
            CDSs (total)                      :: 5,357
            Genes (coding)                    :: 5,178
            CDSs (with protein)               :: 5,178
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 53 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 37 of 179
            Pseudo Genes (multiple problems)  :: 23 of 179
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP034682.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..13478
                     /organism="Klebsiella quasipneumoniae"
                     /mol_type="genomic DNA"
                     /strain="D120-1"
                     /isolation_source="feces"
                     /host="swine"
                     /db_xref="taxon:1463165"
                     /plasmid="pD120-1_13kb"
                     /geo_loc_name="China: Sichuan"
                     /collection_date="2018-07"
     gene            complement(303..1411)
                     /locus_tag="ELE18_RS28125"
                     /old_locus_tag="ELE18_28125"
     CDS             complement(join(303..1171,1171..1411))
                     /locus_tag="ELE18_RS28125"
                     /old_locus_tag="ELE18_28125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611473.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="WP_127114126.1"
                     /translation="MKKRFSDEQIICILREAEAGVSARKHAISDATFYTWRKKYGGMEV
                     PEVKRLKSLEEENARLKKLLAEAMLDKEALQVALGPKVLTTDQKREAVEVMCEAKGLSQ
                     RRACRLAGLSLSTCRYSAQRPPADTQLSLRITELALERRRFGYRRIWQLLRREGLCVNH
                     KRVYRIYQLNGLSVKRRRRRKGLATERLPLLRPMAPNLTWSMDFVMDALATGRRIKCLT
                     CVDDFTKECLTVTVAFGISGVQVTRILDSIALFRGYPATIRTDQGPEFTCRALDQWAFE
                     HGVELRLIQPGKPTQNGFIESFNGRFRDECLNEHWFSDIVHARKIIYDWRLDYNECRPH
                     SSLNYLTPAEFAAGWRNGKYEEKPTDITN"
                     /name="IS3 family transposase"
     gene            complement(1872..2569)
                     /locus_tag="ELE18_RS28135"
                     /old_locus_tag="ELE18_28135"
     CDS             complement(join(1872..2320,2320..2569))
                     /locus_tag="ELE18_RS28135"
                     /old_locus_tag="ELE18_28135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743396.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1A family transposase"
                     /protein_id="WP_223417152.1"
                     /translation="MASVSISCPSCSATDGVVRNGKSTAGHQRYLCSHCRKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGCRATARIMGVGLNTILRHFKKLRPQSVTSRIQPGSDVIV
                     CAEMDEQWGYVGAKSRQRWLFYAYDRLRKTVVAHVFGERTMATLGRLMSLLSPFDVVIW
                     MTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKII
                     GHYLNIKHYQ"
                     /name="IS1-like element IS1A family transposase"
     gene            complement(2736..3215)
                     /locus_tag="ELE18_RS29315"
     CDS             complement(2736..3215)
                     /locus_tag="ELE18_RS29315"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_227545131.1"
                     /translation="MKSGAIFTDRKVHEDHLFTLRLLAGSEKISICNSELYFQNVTSGS
                     LTKSPKKIDYLTERYKSYKEAREDIIYKFSQESIVLYDRWSMSSLLQILKENKKMVFQG
                     IMHTAIYRVLWKEKLVILDFVKSKVKKKSRDLRAYPNRDYRIMGNDANLLISHFH"
                     /name="hypothetical protein"
     gene            complement(3283..3615)
                     /locus_tag="ELE18_RS29320"
     CDS             complement(3283..3615)
                     /locus_tag="ELE18_RS29320"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.3"
                     /GO_function="GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="WP_227545132.1"
                     /translation="MMLLSIIIPCYNCESNIEKLLLSIKEQLNETVQLVLINDGSWDAT
                     HDIITKFTSDNPSIKIKYINSINQGAAKARSTGLNYAEGDYVFFCDSDDMISANFISVF
                     TELSSF"
                     /name="glycosyltransferase family 2 protein"
     gene            complement(3612..5078)
                     /locus_tag="ELE18_RS28145"
                     /old_locus_tag="ELE18_28145"
     CDS             complement(3612..5078)
                     /locus_tag="ELE18_RS28145"
                     /old_locus_tag="ELE18_28145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017803471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOP flippase family protein"
                     /protein_id="WP_205837539.1"
                     /translation="MNLKSKTIHAVKWNLLSTVCVTGLGMVSLWILSSLFTTTDYGIIS
                     AALILSTVFSIITDFGISNSVIRSEKLNKYELSSLYIINVFLGIIFCLGLFIFSAQLAS
                     LFNGGIELAKQIKIMSFSLIISSFGGQPRALLARELRFDVISRITILSAIFNFVFAVFL
                     SYVYRSPWCVAVSLLVSSSISSVLYIYSSKGLRDIKLQFFTLKEIKKHVSYGSQLVMDS
                     IINQISVNTYPVLMARLISLTAIGGYNISYNISVALFEKLNPVLSHALFPAFSKIQNDA
                     VSLKKTFLKVTMFSSLVNFPMLLGMMLISDYVVTVFFEQKWQFITPIVQILCLVGAARS
                     LDVPVISVLLAKGQMYRNVYLGVFKLALGIPLTWFLGKQYGLTGIVFSFLCIQIVNLVM
                     GYFFQVRPCLNIKVKSYIYAVFVPLIHTLPMLVTGYLLILFLTITSPILSMVVIIFSCM
                     SVYIASILTSPVKEIKEFNAIVLNNILRNK"
                     /name="MOP flippase family protein"
     gene            5749..6446
                     /locus_tag="ELE18_RS28155"
                     /old_locus_tag="ELE18_28155"
     CDS             join(5749..5998,5998..6446)
                     /locus_tag="ELE18_RS28155"
                     /old_locus_tag="ELE18_28155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743396.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1A family transposase"
                     /protein_id="WP_223417152.1"
                     /translation="MASVSISCPSCSATDGVVRNGKSTAGHQRYLCSHCRKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGCRATARIMGVGLNTILRHFKKLRPQSVTSRIQPGSDVIV
                     CAEMDEQWGYVGAKSRQRWLFYAYDRLRKTVVAHVFGERTMATLGRLMSLLSPFDVVIW
                     MTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKII
                     GHYLNIKHYQ"
                     /name="IS1-like element IS1A family transposase"
     gene            complement(6744..6893)
                     /locus_tag="ELE18_RS28935"
     CDS             complement(6744..6893)
                     /locus_tag="ELE18_RS28935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_164727443.1"
                     /translation="MPSEHDEQRDAADTQLPGEYDLFDLMAQVTEENLHAPVDSGPPVG
                     REAL"
                     /name="hypothetical protein"
     gene            7462..7593
                     /locus_tag="ELE18_RS29440"
                     /old_locus_tag="ELE18_28170"
     CDS             7462..7593
                     /locus_tag="ELE18_RS29440"
                     /old_locus_tag="ELE18_28170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_072013439.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHAKAPLAAATGNRIHGAE"
                     /name="replication initiation protein"
     gene            complement(7966..8663)
                     /locus_tag="ELE18_RS28175"
                     /old_locus_tag="ELE18_28175"
     CDS             complement(join(7966..8414,8414..8663))
                     /locus_tag="ELE18_RS28175"
                     /old_locus_tag="ELE18_28175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558270.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="WP_227545130.1"
                     /translation="MASVSISCPSCSATEGVVRNGKSTAGHQRYLCSHCRKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGCRASARIMGVGLNTILRHFKKLRPQSVNSRIQPGSDVIV
                     CAEMDEQWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERTLATLERLPGLLSPFDVVIW
                     MTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVI
                     GHYLNIKHYQ"
                     /name="IS1 family transposase"
     gene            complement(8728..9666)
                     /locus_tag="ELE18_RS28180"
                     /old_locus_tag="ELE18_28180"
     CDS             complement(8728..9666)
                     /locus_tag="ELE18_RS28180"
                     /old_locus_tag="ELE18_28180"
                     /inference="COORDINATES: protein motif:HMM:NF017515.3"
                     /GO_function="GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide synthesis protein"
                     /protein_id="WP_127114132.1"
                     /translation="MRYISGLKTLAKDFYHYNFSGMNELFSYDDTHEDNIEIFMKDNSF
                     LEIPKVIWMYWDDASIPPYMSLMIDKIRNDNKDHDLVVLNKNNLVSYIEDLKFSSDKYI
                     PIANKSDIIRLELLKRYGGIWIDSSVILTKPISWVHDINKDKKVDLIGYYRDVSTKDRM
                     YPIVESWFLCSQKNNLFINEWLDVLSPLKEMGAKEYFNMIKSRPDYETILQGIHKPDYL
                     LVYLAQQIAMRKINFNLFSKRSESSAFLYQENESWNSSAIARDLCIKKSPITLPPVIKL
                     TNANRCGIEKLIKLKLVKKDSIIGKLMSGAL"
                     /name="capsular polysaccharide synthesis protein"
     gene            complement(9679..10595)
                     /locus_tag="ELE18_RS28185"
                     /old_locus_tag="ELE18_28185"
                     /pseudo=""
     CDS             complement(9679..10595)
                     /locus_tag="ELE18_RS28185"
                     /old_locus_tag="ELE18_28185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004088970.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            complement(11519..11941)
                     /locus_tag="ELE18_RS28190"
                     /old_locus_tag="ELE18_28190"
     CDS             complement(11519..11941)
                     /locus_tag="ELE18_RS28190"
                     /old_locus_tag="ELE18_28190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_127114134.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKDIKVID
                     TSELIRVFGSLDSVIDAVKHDVKSDAESTGINSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKHEPSSPPHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(11941..12132)
                     /locus_tag="ELE18_RS28195"
                     /old_locus_tag="ELE18_28195"
     CDS             complement(11941..12132)
                     /locus_tag="ELE18_RS28195"
                     /old_locus_tag="ELE18_28195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000166328.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(12612..12761)
                     /locus_tag="ELE18_RS28940"
     CDS             complement(12612..12761)
                     /locus_tag="ELE18_RS28940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_164727443.1"
                     /translation="MPSEHDEQRDAADTQLPGEYDLFDLMAQVTEENLHAPVDSGPPVG
                     REAL"
                     /name="hypothetical protein"
     gene            13330..13461
                     /locus_tag="ELE18_RS29445"
                     /old_locus_tag="ELE18_28210"
     CDS             13330..13461
                     /locus_tag="ELE18_RS29445"
                     /old_locus_tag="ELE18_28210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_072013439.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHAKAPLAAATGNRIHGAE"
                     /name="replication initiation protein"
     Typing          complement(6887..7101)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="81.364"
                     /coverage="100.0"
                     /evalue="3.53e-41"
     Typing          complement(12755..12969)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="81.364"
                     /coverage="100.0"
                     /evalue="3.53e-41"
     Typing          7026..7571
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.922"
                     /coverage="90.0"
                     /evalue="0.0"
     Typing          12894..13439
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.922"
                     /coverage="90.0"
                     /evalue="0.0"
ORIGIN
        1 gttcttgaag tggtggcctg actgcggcta cactagaagg acagttttgg tatctgcgct
       61 ccgtgaaagc cagttacctt cggaaaaaag agttggtagc tcttgattcc ggcaaacgaa
      121 ccaccgttgg tagcggtggt ttttttgttt gcaggacatg agattacgac gttcaaaaac
      181 ggatctcaag aagatccttt caagatctac gacgatatca atgttcaggc tcatcaaaac
      241 catcattgac tgaagagctg gattggcccc tattgacctg cccccagagt tagatacaac
      301 cttcagttag taatgtcggt tggtttttct tcatatttcc cgtttcgcca gcccgctgca
      361 aattcagccg gcgtcaggta attcagtgat gaatgtggtc gacactcgtt ataatccagt
      421 cgccagtcat aaatgatctt cctggcgtga acaatatcgc tgaaccagtg ctcattcagg
      481 cattcatcac gaaagcgtcc gttaaaactc tcaataaatc cgttctgcgt tggcttgccg
      541 ggctggataa gtcgcagctc cacaccatgc tcaaaagccc actgatcgag tgcgcggcag
      601 gtaaactccg ggccctgatc ggttcttatc gtagccggat agccgcgaaa cagcgcaatg
      661 ctgtccagaa tacgcgtgac ctgcacgcct gaaatcccga aggcaacagt gaccgtcagg
      721 cattcctttg tgaaatcatc cacgcaggtc aggcacttga tcctgcgacc tgtggccagt
      781 gcgtccatga cgaaatccat tgaccaggtc agattgggcg ccatcgggcg gagcagcggc
      841 agacgttctg ttgccagccc tttacgacgt cgtctgcgtt ttacactcag gccattaagt
      901 tgatagatgc ggtaaacccg cttgtggtta acgcaaagac cttcacgtcg cagaagctgc
      961 cagatacgcc ggtaaccaaa acggcggcgt tcaagtgcca gctctgtgat gcgtagagac
     1021 agctgcgtgt cagcaggcgg acgctgagcc gaatatcggc aggttgacag ggacagacct
     1081 gccagcctgc aggcacgacg ttgcgacaga cccttagcct cgcacatgac ttccacggct
     1141 tcccgcttct ggtctgtcgt cagtacttta gcccaagagc cacctgaagc gcctccttat
     1201 ccagcatggc ttcagcaagc agcttcttga gtctggcgtt ctcttcctca agagacttca
     1261 ggcgcttaac ctcaggcacc tccataccgc catacttctt acgccaggtg taaaaggtgg
     1321 cgtcggaaat ggcgtgctta cgggcagaaa cgccggcttc ggcctcgcgg agaatacaga
     1381 tgatctgttc gtcggaaaaa cgcttcttca tggggatgtc ctcatgtggc ttatgaagac
     1441 attactaaca tcgcggtgta ttaatcaacg gggagcaggt caacgttacg caatgagccc
     1501 tgcgcttatg gcgggctgtt gtctggcacc gttcatgtgc taatatcctt tcaaggtttc
     1561 gacctggccc tggttatcat gtccgctcgc caaagtagag catgatttcc cgggctttgt
     1621 tttttctggc cttaaagtta caccgtctta cgctatctgt cgatctcccc cctgattgaa
     1681 cataacatcc cttgcgcgca ccaccaccac accactcatc gccaaaatcc gccggaacgt
     1741 ccaaaagtgc aggttttaca caccgaaatc gggctatttt tgtgttgccg gtttgttatc
     1801 aaaatggcga gactggtttc agcctgttta cagactgaaa cctctatttc ttccagggta
     1861 atgactcaaa attattgata gtgttttatg ttcagataat gcccgatgat tttgtcatgc
     1921 agctccaccg attttgagaa cgacagcgac ttccgtccca gccgtgccag gtgctgcctc
     1981 agattcaggt tatgccgctc aattcgctgc gtatatcgct tgctgattac gtgcagcttt
     2041 cccttcaggc gggattcata cagcggccag ccatccgtca tccatatcac cacgtcaaag
     2101 ggtgacagca ggctcataag acgccccagc gtcgccatag tgcgttcacc gaatacgtgc
     2161 gcaacaaccg tcttccggag cctgtcatac gcgtaaaaca gccagcgctg gcgcgattta
     2221 gccccgacat agccccactg ttcgtccatt tccgcgcaga cgatgacgtc actgcccggc
     2281 tgtatgcgcg aggttaccga ctgcggcctg agttttttaa gtgacgtaaa atcgtgttga
     2341 ggccaacgcc cataatgcgg gcggttgccc ggcatccaac gccattcatg gccatatcaa
     2401 tgattttctg gtgcgtaccg ggctgagagg cggtgtaagt gaactgcagc tgccatgttt
     2461 tacggcagtg agagcagaga tagcgctgat gtccggcagt gcttttgccg ttacgcacca
     2521 ccccgtcagt agctgaacag gagggacagc tgatagaaac agaagccact ggagcacctc
     2581 aaaaacacca tcatacacta aatcagtaag ttggcagcat cacccagaaa gaccataaaa
     2641 ttagcctata acggcaaata gtaatgctgc caacctgttg gtgaccattt aaatctgtag
     2701 ccattcaatc tttgtaacat caggtgatcc tggcgtcagt gaaaatgcga aatcaacaaa
     2761 ttggcatcat tacccattat acgataatcc ctattgggat aggctcttag atctcgtgat
     2821 ttttttttaa ctttgctttt aacaaaatct aaaatcacta atttttcctt ccacaatact
     2881 cgatatatag ctgtatgcat tatcccctga aacaccattt ttttattttc ttttaatatt
     2941 tgcagtagcg aagacataga ccatcgatca taaagaacaa tgctttcctg actgaatttg
     3001 taaatgatat cttccctggc ctctttataa gacttataac gctcagttaa ataatcaatt
     3061 tttttagggg atttagtaag tgaacctgaa gtcacattct gaaagtaaag ctcagaatta
     3121 catattgata ttttttcact accagcgaga agtctcagcg tgaataagtg atcttcatgt
     3181 acctttctgt ctgtaaatat agcgccagat ttcaatacga gatctttccg gaaaatataa
     3241 gtccatacag cagctgtcca ctgccctttc ttcaaaagat aattaaaaac tacttaactc
     3301 tgtaaaaacc gatatgaaat tagcagaaat catatcatct gaatcacaga agaatacata
     3361 atctccctct gcgtaattta agcccgttga tctggctttt gccgccccct gattaatact
     3421 attaatgtat tttattttta tagaggggtt gtcacttgta aatttagtta ttatgtcatg
     3481 cgttgcgtcc catgagccat cattaatcaa tactagttgt acggtttcat taagctgctc
     3541 ttttattgag agaagcaatt tttcgatatt tgactcacag ttataacatg gtatgattat
     3601 tgataataac atcatttatt cctcagtata ttgtttagaa cgattgcgtt aaattccttt
     3661 atctctttta ctggagaggt gagtatagaa gcaatgtata cagacataca tgaaaaaata
     3721 atcacaacca ttgacaaaat aggagaggta atagttaaaa aaagaatcaa caggtaaccc
     3781 gttacaagca taggtaaagt gtgtataagc ggtacgaaga cggcatagat gtaactcttt
     3841 actttgatat ttaaacaggg tctaacctgg aaaaaatatc ccataacaag atttactatt
     3901 tgaatacata agaatgaaaa cactatccct gtaagaccat attgtttacc cagaaaccat
     3961 gttaaaggaa taccaagcgc taacttaaat actcccaggt aaacatttct gtacatttgc
     4021 cctttagcaa gcaagacaga gataacaggc acatcaaggg atcgcgccgc cccaaccaaa
     4081 cataaaatct gtacaatagg ggtgatgaac tgccatttct gctcaaaaaa aacagtcact
     4141 acatagtctg aaattaacat cattcccaat aacatcggga aattcaccaa agacgaaaac
     4201 atagttactt ttagaaaagt ttttttcaga gatactgcat cattttgtat cttggaaaaa
     4261 gctggaaata acgcatgtga taaaacagga ttcaattttt caaaaagcgc cacgctaatg
     4321 ttgtaggata tattatatcc accgatagca gtcaaactta ttaagcgtgc cattagtaca
     4381 ggatatgtat tcacgcttat ctgattaatg atagagtcca tgaccaactg agaaccgtag
     4441 ctgacatgtt tttttatttc cttcaaagta aagaactgta acttaatatc tctcaagcct
     4501 ttgcttgaat atatatacaa tacgctacta atagatgagg aaaccaacag tgaaacggca
     4561 acacaccagg gggaacgata gacgtagctt aagaagactg cgaacacaaa attaaaaata
     4621 gctgacagga ttgttatcct ggatattaca tcaaatctaa gttctctggc taacaaagct
     4681 cttggctgtc caccgaaaga cgatattata agagaaaatg acatgatttt tatctgtttt
     4741 gccagttcta ttccaccatt gaataaggat gctaactgag cactaaaaat aaacaagccg
     4801 agacaaaata ttatacctaa aaagacattt attatataca gtgaagacag ttcatattta
     4861 tttaactttt cagatcttat aactgagttt gatataccaa aatcagttat gattgagaaa
     4921 acagtactta atattaatgc cgcactaatg ataccataat ctgtcgttgt aaataagctg
     4981 gacaagatcc acagtgagac cataccaagc ccggttacgc acactgtaga caacaaattc
     5041 cacttcacag catgtattgt tttcgatttt aaattcatat ttattgtcag tgatataaaa
     5101 taattacatt ggtgtactaa aaggaatttt ttataccttt cagcgttaga gcctatccca
     5161 aagtaatgct gccaacctgt tggtgaccat ttgaatctgt agccattcaa tccttgtaat
     5221 atcaggtgat cctggcgtca gtgaaaatgc gaaatcaata aattggcatc attaccatca
     5281 tcagcatata caagaatgtc atcaatctcc gagttaataa ttgcgttaat tattttacgg
     5341 tatgggccgg tattttttac ataatgtaca ttcactatgt tatttatttt atttagctcc
     5401 tcaacccaag caggtatctc atatatccca tgatctgaca aatatggctc atttgatatc
     5461 caaaggttta ttttatctgg ctgaacactc tggtttataa ttgaccacac tgtggcggca
     5521 cataaatcca gtcgagaatg cattgtagtt aaattaactg atatcatgct accctttaaa
     5581 gaaaaaatta atttaatgtt atattatatg taaaaaggtt acatattcaa tatgtaggac
     5641 gttttttcga aaaaatcagt ttttttcctg atgaaccttc ctccgcgttg cccggtgatg
     5701 ctgccaactt actgatttag tgtatgatgg tgtttttgag gtgctccagt ggcttctgtt
     5761 tctatcagct gtccctcctg ttcagctact gacggggtgg tgcgtaacgg caaaagcact
     5821 gccggacatc agcgctatct ctgctctcac tgccgtaaaa catggcagct gcagttcact
     5881 tacaccgcct ctcagcccgg tacgcaccag aaaatcattg atatggccat gaatggcgtt
     5941 ggatgccggg caaccgcccg cattatgggc gttggcctca acacgatttt acgtcactta
     6001 aaaaactcag gccgcagtcg gtaacctcgc gcatacagcc gggcagtgac gtcatcgtct
     6061 gcgcggaaat ggacgaacag tggggctatg tcggggctaa atcgcgccag cgctggctgt
     6121 tttacgcgta tgacaggctc cggaagacgg ttgttgcgca cgtattcggt gaacgcacta
     6181 tggcgacgct ggggcgtctt atgagcctgc tgtcaccctt tgacgtggtg atatggatga
     6241 cggatggctg gccgctgtat gaatcccgcc tgaagggaaa gctgcacgta atcagcaagc
     6301 gatatacgca gcgaattgag cggcataacc tgaatctgag gcagcacctg gcacggctgg
     6361 gacggaagtc gctgtcgttc tcaaaatcgg tggagctgca tgacaaaatc atcgggcatt
     6421 atctgaacat aaaacactat caataatttt gagtcattac ccatgcgagc atgaacgagg
     6481 tcgctaagga cgctttttcg tcaccgatgc tccttaacct gcgcagtaca cccggccaga
     6541 atgataattt tttgcccctt aaatggtcat acatctaacg cagccgcgat tttcatcgca
     6601 actgcatgaa ttaaaaggta aaaactgaaa tccccgttta tttatgtaat acgcaacgta
     6661 ttacaatcac ctgtaatcac atgaaaataa agaaataaaa tgtaataccg gcgtattacc
     6721 acgtacgcgg gcaagcgaaa aagctataac gcttcccggc cgacaggtgg cccggaatcc
     6781 acaggggcat gcaggttctc ttcagtaacc tgcgccatta aatcaaaaag gtcatattcg
     6841 ccgggaagct gcgtatcagc agcatcccgt tgttcatcat gttcagatgg catgggatga
     6901 tatccagtag ctgagaggtt gttagcgggt ttcggggcgc aaccctgaac cagtcacgta
     6961 gcgctagcag agtgtataat ggcttattat gttggcatga gtgcgataat caggaaagcg
     7021 cctcatgtgg caggagaaaa atgctgcagc agagcgtaag cagaatatgc gatacagaag
     7081 atattccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     7141 acagacttac ggacgggcgg agatttcctg gaagatgcca gagagaagct taacagggaa
     7201 gcgataaggt ctcggcgaag ccgtttttcc ataggctccg ccccccctga caagcatcac
     7261 gaaatctgac gctcaaatca gtggtggcga aacccgacag gacttaaaga taccagacgt
     7321 ttccccctgg tggctccctc gtgcgctctc ctgttcctgc ctttcggttt accggtgtca
     7381 ttccgctgtt atggccgcgt ttatctcatt ccacgcctga cactcagttc cgggtaggca
     7441 gttcgctcca agctggactg tatgcacgaa ccccccgttc agtccgactg ctgcgcctta
     7501 tccggtaact atcgtcttga gtccaacccg gaaagacatg caaaagcgcc actggcagca
     7561 gccactggta acaggattca cggagcagag tagtagagtg cttctacaga gttcttgaag
     7621 tggtggcctg actgcggcta cactagaagg acagttttgg tatctgcgct ccgtgaaagc
     7681 cagttacctt cggaaaaaag agttggtagc tcttgattcc ggcaaacgaa ccaccgttgg
     7741 tagcggtggt ttttttgttt gcaggacatg agattacgac gcaacagaaa ggatctcaag
     7801 aagttccttg ctgtattgct actatcctag agatctgacc cattaggggc attatcagat
     7861 tacggccttg taatccacga taaaaaccca gaaaccagaa gagttggccc agcgacatat
     7921 gttacataaa cgcccacata tgaaactgta ggtaatgact ccaacttact gatagtgttt
     7981 tatgttcaga taatgcccga tgactttgtc atgcagctcc accgattttg agaacgatag
     8041 tgacttcctg cccagccttg ccagatgctg cctcagattc aggttatgcc gctcaatgcg
     8101 ctgcgtatat cgcttgctga ttacgtgcag ctttcccttc aggcgggatt catacagcgg
     8161 ccagccatcc gtcatccata tcaccacgtc aaagggtgac agcaggcccg gaagacgctc
     8221 cagcgtggcc agcgtgcgtt caccgaatac gtgcgccaca accgtcctcc gtatcctgtc
     8281 atacgcgtaa aacaaccagc gctggcgtga tttagcgccg acgtaacccc actgttcgtc
     8341 catttccgcg caaacaatga cgtcactgcc cggttgtatg cgtgagttta ccgactgcgg
     8401 cctgagtttt ttaagtgtcg taaaatcgtg ttgaggccaa cgcccataat gcgtgcactg
     8461 gcgcgacatc cgacgccatt catggccata tcaataattt tctggtgtgt accgggctga
     8521 gaggcggtgt aagtgaactg tagctgccat gttttacggc agtgagagca gagatagcgc
     8581 tgatgtccgg cagtactttt accgttacgc accacgcctt cagtagctga acaggaggga
     8641 cagctgatag aaacagaagc cactggagca cctcaaaaac accatcatac actaaatcag
     8701 taagttggca ccattaccgc cttctcgtta gagtgctcct gacattaatt ttccaatgat
     8761 gctatctttc tttactaatt ttaattttat gagcttttca attccgcatc tattagcatt
     8821 ggttagtttt attactggtg gtaatgttat cggagatttt ttaatgcaaa gatcccttgc
     8881 tatagctgaa gagttccaac tctcattctc ttgatataag aatgcactgg actcgcttct
     8941 tttagaaaat aaattaaagt taatttttct cattgcaatt tgctgtgcga ggtaaacaag
     9001 taaatagtca ggcttgtgaa tgccttggag aatagtttca tagtcgggcc tggattttat
     9061 catattaaaa tattcctttg cacccatttc ttttaatggc gaaagaacat caagccattc
     9121 attaatgaac aaattatttt tctgtgagca taaaaaccag ctttcaacaa taggatacat
     9181 tctatctttc gttgagacat ccctataata accaataaga tcgacttttt tatctttatt
     9241 aatatcatga acccatgata tgggctttgt caggataaca gaagagtcaa tccatatccc
     9301 tccgtaacgc tttaataatt caagtcttat tatgtctgac ttgtttgcta taggaatgta
     9361 tttatcagaa gaaaatttca aatcttcaat gtaactgact aaattatttt tattcaaaac
     9421 aactaaatca tgatctttgt tgtcatttct tattttatct atcatcagac tcatgtaagg
     9481 cggaatacta gcgtcatccc agtacatcca tattaccttc ggtatttcca ggaaagaatt
     9541 atctttcata aatatttcta tgttatcttc atgtgtatca tcatagctaa acaactcatt
     9601 cattccggaa aaattataat gataaaaatc ttttgcaagt gtttttaacc cactaatgta
     9661 tctcatacac accctatttc aattaatttt aaaatacttc cgataccaag tgctacgact
     9721 aatacccaaa tcaatccatg gactactttt tcttttaggc ctccctcctc cacggcttat
     9781 tacaccacca atctcccccc cttccttcct ctttcagatt gaatttttga catatgtgta
     9841 ctttttacaa attcataaaa tgattctgcc gtaaattccc cgtgagtcca cttagcaatg
     9901 cttttagcga tcccattcac ctcattttca tccagaggag ctgaaaattg cagattatag
     9961 gcgtaggcac gttcgtagca ggcttgcagc cactggtcgt attcaggcca tccctgacga
    10021 atagcgcgat acgcccactt tctggttttg tcgaacagag tacagttccg gcctaagcca
    10081 tagtcagcaa caatctcttt gtcgttcgcg gcgttcagat cgagtgagtc ggctaaccag
    10141 tccagcgtgt atagttcagg ttgccagacg gcaattttcc agtagctgtg gtttggattc
    10201 ttgcaaatca gaccagaata gcccgtgtca gcgtccagtt ttttacgcag cgcgttctcc
    10261 acggcagcgg catatttcag cggcttcatt tttccgtccg gcgcagtgcg taccgaggtt
    10321 ttcagggcgt acagcaggtg cgcgtgtccg ttttcaggat tggtgatggt cagcgtaggc
    10381 gcaggagcgt tgcgatcgct ccagtctata gccgcgccga cgcgatcgac gtcaaatccc
    10441 agccagaaca tggcatgagg ctggttgaac tggatgtatt tcgccaggat tgcgcgctct
    10501 ttccctgcga tgcgtacacc gaagtgaaga tcgtccgaga agtacggctt atggggtaag
    10561 cggtcgttaa aaagttcgag tgcagcgtta tgcaatgtgc cctgcgctta tggcgggctg
    10621 ttgtctggca cggttcatgt gctaatatcc tttcaaggtt tcgacctggc cctggtaatc
    10681 atgacggtcc gccaagatat gacatgattt ccggggattt gttttttctg gccttaaagt
    10741 tacaccgtct tacgctatct gtcgatctca ctgcgattta ccataacatc cgttgcccgc
    10801 accgcctgag aggccctcag ggcgtttctg acgggaaacc ggcaaaacag gcaacaaacc
    10861 acccgaaaac ccgccagaaa cgcgcaggct ctgtttttac ggcatgcatg agtatgcgcg
    10921 caagtgcata gcgcggaaat tcacgctatt cagccagggt aagtcctgtt tccggcgttc
    10981 ttcccatccg aaaacggtga acggggacgc aatttattgc gtggccgtga agcgttgccg
    11041 gcgcagccgg aaaaccggtt ttcagcacgt tcaggaatcg ggttcgtcga cggccggcaa
    11101 tcacgttatg gcaagtcgcc ggacgactca ccacacgatt caccggcgat tcgactcgtg
    11161 acttgactct agagtcacca cggtcataaa aacgccaaag cgacgattcg actctagagt
    11221 caagtcgtca taagttactg atagtgtgga gtgagtcgtc gatgactcta tgacgagtcg
    11281 tcggcaagtc gtacacgaat caccgacgac ttgaattgac cttacagaag gtaatactgc
    11341 gtaatttaat aacaaccaac tgactgatat taatgagtta tcttcaaaat tttaattttg
    11401 cgtggggtgt gggtatgggt atttttagtg gtgaggagcg ttcagagatc tggcggtatc
    11461 tgggaaggtt caggaacgcc ccgggatgac cggagaggaa aactgaagca tggcgggatc
    11521 aggatttttt ccagaatttc caccacgggg aatggggtgg tgaagaaggt tcgtgcttgt
    11581 gctccagaag ctgcatagcc tgacgaaggg aatcaatatg ctcatctcga gccttaacag
    11641 cccctttaag accatcgttt tcagcttcaa gtaaagcgat tctatgttgt aaatcatgta
    11701 aaccaacgct gttaatacct gtagattctg cgtcactttt tacatcatgt tttacagcat
    11761 cgattacact atcaagagaa ccaaacactc taataagctc tgatgtatca ataactttaa
    11821 tatccttccc gtgctttaca acagaggtgt aacttaactt accactgtta atcatcttgt
    11881 aaatagtagg gcgactaacc ttaacaagcc gtgctgcttc actaatagac actctggcca
    11941 tcagttacca tcctgtaatg catccagtct cgaggacagt gtacggaaaa gatgctccgc
    12001 atcatcgtgg agtttctcac aaaggtcggc ctcagcatcg agatcaagct cgttaagctt
    12061 ctccagcagg agcagagact gattctggat gaatttagcc atgttcagcg ctgttttctg
    12121 ttgtttcgtc ataagggccc tcagagcatc agtgctcagt gtaaaccggt aagggggtcg
    12181 gatgtacaaa tggttcagag aacggatctg aggtaatcct gtagttattt tgtagtttat
    12241 ctgtagtcat cgcactaaaa atcaggatta aatcatcaac ttttgtagtc ttcctgtagt
    12301 ttacctgtag tccggaaaaa taatccgttc atgcgagcat gaacgaggtc gctaaggacg
    12361 ctttttcgtc accgatgctc cttaacctgc gcagtacacc cggccagaat gataattttt
    12421 tgccccttaa atggtcatac atctaacgca gccgcgattt tcatcgcaac tgcatgaatt
    12481 aaaaggtaaa aactgaaatc cccgtttatt tatgtaatac gcaacgtatt acaatcacct
    12541 gtaatcacat gaaaataaag aaataaaatg taataccggc gtattaccac gtacgcgggc
    12601 aagcgaaaaa gctataacgc ttcccggccg acaggtggcc cggaatccac aggggcatgc
    12661 aggttctctt cagtaacctg cgccattaaa tcaaaaaggt catattcgcc gggaagctgc
    12721 gtatcagcag catcccgttg ttcatcatgt tcagatggca tgggatgata tccagtagct
    12781 gagaggttgt tagcgggttt cggggcgcaa ccctgaacca gtcacgtagc gctagcagag
    12841 tgtataatgg cttattatgt tggcatgagt gcgataatca ggaaagcgcc tcatgtggca
    12901 ggagaaaaat gctgcagcag agcgtaagca gaatatgcga tacagaagat attccgcttc
    12961 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtac agacttacgg
    13021 acgggcggag atttcctgga agatgccaga gagaagctta acagggaagc gataaggtct
    13081 cggcgaagcc gtttttccat aggctccgcc ccccctgaca agcatcacga aatctgacgc
    13141 tcaaatcagt ggtggcgaaa cccgacagga cttaaagata ccagacgttt ccccctggtg
    13201 gctccctcgt gcgctctcct gttcctgcct ttcggtttac cggtgtcatt ccgctgttat
    13261 ggccgcgttt atctcattcc acgcctgaca ctcagttccg ggtaggcagt tcgctccaag
    13321 ctggactgta tgcacgaacc ccccgttcag tccgactgct gcgccttatc cggtaactat
    13381 cgtcttgagt ccaacccgga aagacatgca aaagcgccac tggcagcagc cactggtaac
    13441 aggattcacg gagcagagta gtagagtgct tctacaga
//
