LOCUS       NZ_CP034680             3897 bp    DNA     circular CON 26-NOV-2023
DEFINITION  Klebsiella quasipneumoniae strain D120-1 plasmid pD120-1_3kb,
            complete sequence.
ACCESSION   NZ_CP034680
VERSION     NZ_CP034680.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN10622699
            Assembly: GCF_003990375.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella quasipneumoniae
  ORGANISM  Klebsiella quasipneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3897)
  AUTHORS   Yang,X., Bai,L. and Li,R.
  TITLE     Emergence of mcr-8.2-bearing Klebsiella quasipneumoniae of animal
            origin
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3897)
  AUTHORS   Yang,X., Bai,L. and Li,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2018) College of Veterinary Medicine (Institute of
            Comparative Medicine), Yangzhou University, Wenhui Road, Yangzhou,
            Jiangsu 225009, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: NOV-2018
            Assembly Method                   :: Unicycler v. 0.4.4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/26/2023 02:49:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,478
            CDSs (total)                      :: 5,357
            Genes (coding)                    :: 5,178
            CDSs (with protein)               :: 5,178
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 53 of 179
            Pseudo Genes (incomplete)         :: 114 of 179
            Pseudo Genes (internal stop)      :: 37 of 179
            Pseudo Genes (multiple problems)  :: 23 of 179
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP034680.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3897
                     /organism="Klebsiella quasipneumoniae"
                     /mol_type="genomic DNA"
                     /strain="D120-1"
                     /isolation_source="feces"
                     /host="swine"
                     /db_xref="taxon:1463165"
                     /plasmid="pD120-1_3kb"
                     /geo_loc_name="China: Sichuan"
                     /collection_date="2018-07"
     gene            12..764
                     /locus_tag="ELE18_RS26460"
                     /old_locus_tag="ELE18_26460"
     CDS             12..764
                     /locus_tag="ELE18_RS26460"
                     /old_locus_tag="ELE18_26460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001711308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023307481.1"
                     /translation="MGIENSNQGPGWHDHDTPYGTIHSYDGDTGTYGGGGTGGNAGGGH
                     TGLTANTWVDIATTKDYNGRYMSMCLNNLKDHEGSINTMYRDSKGNITVGIGHYLGSAE
                     MAQALPFNRTKVMHVHGDDLESEVAVSKSDIASAFNAYKSNPRDVPSMHLSNADVIGQC
                     IKDVQTTENGLRGLYPGYDRFSDPRKTALVDMGFNLGIPTLKSKFPKFNDAVNRGDWNV
                     AADESHRDLNQRGDKRNKDTADQLRYNR"
                     /name="hypothetical protein"
     gene            866..1366
                     /locus_tag="ELE18_RS26465"
                     /old_locus_tag="ELE18_26465"
     CDS             866..1366
                     /locus_tag="ELE18_RS26465"
                     /old_locus_tag="ELE18_26465"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_123829470.1"
                     /translation="MIRFVIAAFISILTIKGAFADINVFIAPNDLNYETNSTSEINHYL
                     DELKKNNTKVDVDKDPKISNELKKYIGIVNDRVSDVFSESEFKKYRGDVCKISITTGNT
                     APSDPVLTQVFTYDELADNKKQSCQKLNNELIKKVAKDKGLSYPKVVKDYGSLSIDMIF
                     LFK"
                     /name="hypothetical protein"
     gene            1414..1548
                     /locus_tag="ELE18_RS26470"
                     /old_locus_tag="ELE18_26470"
     CDS             1414..1548
                     /locus_tag="ELE18_RS26470"
                     /old_locus_tag="ELE18_26470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724569.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_110216848.1"
                     /translation="MSICVIILLAACQANYIRDVKGGTVAPSASSDIRTSSSQPIGSS"
                     /name="colicin release lysis protein"
     gene            complement(1713..1855)
                     /locus_tag="ELE18_RS29425"
                     /old_locus_tag="ELE18_26475"
                     /pseudo=""
     CDS             complement(1713..1855)
                     /locus_tag="ELE18_RS29425"
                     /old_locus_tag="ELE18_26475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            2271..2462
                     /locus_tag="ELE18_RS26485"
                     /old_locus_tag="ELE18_26485"
     CDS             2271..2462
                     /locus_tag="ELE18_RS26485"
                     /old_locus_tag="ELE18_26485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004187666.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2462..2863
                     /locus_tag="ELE18_RS26490"
                     /old_locus_tag="ELE18_26490"
     CDS             2462..2863
                     /locus_tag="ELE18_RS26490"
                     /old_locus_tag="ELE18_26490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_049088770.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGLVSEQRDTASADHSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     Typing          complement(1536..2037)
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.415"
                     /coverage="99.0"
                     /evalue="1.02e-154"
ORIGIN
        1 aggtaatgtt tatgggtatt gaaaattcaa atcaggggcc aggatggcat gatcatgata
       61 ctccttatgg cactattcac agctatgatg gtgatactgg tacgtatgga ggtggtggaa
      121 ctggagggaa tgctggtggt gggcataccg gtcttactgc aaatacatgg gtagatatcg
      181 cgacaacaaa agattataac ggtagatata tgtctatgtg tttaaataat cttaaagacc
      241 atgaaggttc aattaataca atgtatagag actctaaagg taatattacc gttggtattg
      301 gtcattacct tggttctgct gagatggctc aggcattacc ttttaatcga acaaaagtta
      361 tgcatgtgca tggtgatgat cttgaaagtg aggtcgccgt atctaagtct gatatagctt
      421 cagcatttaa tgcatacaaa tcaaacccaa gagatgttcc tagtatgcat ctttcaaatg
      481 ctgatgtaat aggtcagtgt attaaggatg ttcaaactac tgaaaatggt ttgcgtggac
      541 tttacccagg ttatgatcgt ttttcagatc cacgaaaaac agcattggta gatatgggtt
      601 ttaatcttgg tattcctacg cttaagtcta aatttccgaa gtttaacgat gcagttaatc
      661 gtggtgattg gaatgttgct gcagatgaaa gtcatcgaga tcttaatcaa agaggtgata
      721 agcgtaataa agatacggca gatcaattgc gttataatcg gtaatgattt agatttacaa
      781 ctcctgccat caaatggcag gagtgtcctc acgatattaa aactaagtca tgaggtttct
      841 aatcttatat tttatgggtg gtattatgat tcgttttgtg attgctgctt ttatttcaat
      901 cttgacaatt aaaggagctt ttgctgatat caatgttttt atagcgccta acgatctaaa
      961 ttatgaaaca aattctacta gtgaaattaa tcattattta gatgagctga aaaaaaataa
     1021 tactaaagtt gatgttgata aagacccaaa aattagcaat gagttaaaaa aatatattgg
     1081 tattgttaac gatagagtgt ctgatgtttt cagtgaaagt gaatttaaga agtatcgtgg
     1141 cgatgtctgt aaaatatcaa taacaactgg gaatacggct ccatctgatc ctgttttgac
     1201 tcaagtgttt acttatgatg aactagctga taataaaaaa caatcatgtc aaaaattaaa
     1261 taatgagcta ataaaaaaag ttgctaaaga taagggttta tcatatccga aagttgttaa
     1321 agattatgga tctttatcaa ttgatatgat ctttttattt aaataaaaat aaatttacgg
     1381 tgttgtttat gccttttaaa aaagtctttt ccaatttcta tttgtgtaat tatattgttg
     1441 gctgcctgcc aagctaacta tattagggac gtaaaaggag gaactgttgc tccatcagct
     1501 tcttcagata taagaacgtc gagttcgcaa cccataggat cttcttgaga tccttagcgg
     1561 atagtcgtaa tctttgctct gtaaacgaaa aaaccacccg gcaaggtggt tttttcgaag
     1621 gttcattaac cggcaagttt ctgaaccgtg gtaactggct ttcgcagagc acagaaaccg
     1681 aattagttct aacagtgtag ctgtagttta cctcacactt caacacttct ttgttctgcg
     1741 aatgcgttac cagtggctgc tgccagtggt gataagtcgt gtcttttcgg gttggactca
     1801 agttgatagt taccgaaaag gcgcagcagt cggactgaac ggggggttcg tgcatacagt
     1861 ccagcttgga gcgaactgcc tacccggaac tgagtgtcag gcgtggaatg agataaacgc
     1921 ggccataaca gcggaatgac accggtaaac cgaatggcag gaacaggaga gcgcacgagg
     1981 gagccatcag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccaccactga
     2041 tttgagcgtc agattccgtg atgctcgtca ggggggcgga gcctatggaa aaacggccgc
     2101 cttgcggcct ttccttcctc tcctccgttg aacgctctgc tttctcgagc ttttatacgg
     2161 atgctcctgc cgctcgccgc agccgaacgg cggaagcgta gcgactgagt gagcgaggaa
     2221 gcggaaaaga gaacatccct atactgagca ctgacgctct gaggcctctt atgacgaaac
     2281 aacagaaaac agcgctgaac atggctaaat tcatccagaa tcagtctctg ctcttgctgg
     2341 agaagcttaa cgagcttgat ctcgatgttg aggccgacct ttgtgagaaa ctccacgatg
     2401 atgcggagca tcttttccgt acactgtcct cgagactgga tgcattacag gatggtaact
     2461 gatggccaga gtgtctatta gtgaggctgc ccgactgacg ggtaagagta gaacaacact
     2521 gcacaggtta attaaagcag gtgacctgtc cacctgtagc ggtgaacgca atgcaaaaat
     2581 ggtagatact tcagagcttt tacgtgtctt tggtcccttt gagcaaccga agggtgaaca
     2641 ccattctgga ctggtgtctg aacagcgtga tacagcctct gctgaccata gtgaacaggt
     2701 gattcgtcat ctgaaacagg agattgaaca cctgaatacc ttacttttgt ccaaggattc
     2761 gcacattgac agcctgaaac aggccatgct ccttcttgaa aatcaccagg caaaattaac
     2821 tgaatccccg gcgccatggt ggaaattctg gaaaaaatcc tgaccccgtc gtgcttcagt
     2881 tttcctctcc ggccatcccg gggagttcct gaaccttccc aaaaatcgcc agatctctga
     2941 acgctcttca ccactaaaaa tacccacaca ctacgcaaaa ttaaaatttt gcagatttgt
     3001 atttaatatc agtagtttac tttcatgaaa tttcgttttt taaattttat ttacatagat
     3061 ccgactctat gacgactcgt catagagtcc ccggcgagtc gtcatagagt cggtaacgac
     3121 tcaggactat aaaaacaatg agttaataac gacccgactc tagacccgac tccccgtttg
     3181 gttgtttttt agacgcggca actctgggta cgactctagt gaggagtcgc cggtgaatcg
     3241 tgtggtgagt cgtccggcga cttgccataa cgtgattgcc ggccgtcgac gaacccgatt
     3301 cctgaacgtg ctgaaaactg gttttccggc tgcgccggca acgcttcacg gccacgcaat
     3361 aaattgcgtc cccgttcacc gttttcggat gggaagaacg ccggaaacag gacttaccct
     3421 ggctgaatgg cgtgaatttt ctcgctatgc acttgcgtgc atactcatgc atgccttaaa
     3481 aacagagcct gcgcgtttct ggcgggtttt cgggtggttt gttgcctgtt ttaccggttt
     3541 cccgtcagaa acgccctgag gccgttttag cggtgcgtac aattatggct ttatgttaaa
     3601 tcattgccgc aaacagccac gaaaagctta cttggcatcg gctctactag cctgttaaaa
     3661 ctcatatttt gaactcaatc atttaaattt cgtcttttca ggcgtacaac ttcgataact
     3721 aaaaatttat gtgaaataga tatttatcca acagttatgt agataagaat aaaacatcaa
     3781 tatttttttg agagtgtcaa ggaagttata gtgagaattt ttctaaaagt attgacagat
     3841 tcaaagaaga tgaacaaatt tatactgttt atttatacag ttgttttttt gtcaaag
//
