LOCUS       NZ_CP034557             3849 bp    DNA     circular CON 17-MAY-2024
DEFINITION  Burkholderia cepacia ATCC 25416 plasmid unnamed2, complete sequence.
ACCESSION   NZ_CP034557
VERSION     NZ_CP034557.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN10591700
            Assembly: GCF_006094315.1
KEYWORDS    RefSeq.
SOURCE      Burkholderia cepacia ATCC 25416
  ORGANISM  Burkholderia cepacia ATCC 25416
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae; Burkholderia; Burkholderia cepacia complex.
REFERENCE   1  (bases 1 to 3849)
  AUTHORS   Chen,H.
  TITLE     Burkholderia cepacia Genome sequencing and assembly
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3849)
  AUTHORS   Chen,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-DEC-2018) Key Laboratory of Microbial Technology and
            Bioinformatics of Zhejiang Province, Zhejiang Tianke High Technology
            Development Co.Ltd., Huanggushan Road, Hangzhou, Zhejiang 310000,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. Unicycler v0.4.5
            Assembly Name                     :: TK42
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 79.0x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_006094315.1-RS_2024_05_17
            Annotation Date                   :: 05/17/2024 02:34:14
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,916
            CDSs (total)                      :: 7,822
            Genes (coding)                    :: 7,695
            CDSs (with protein)               :: 7,695
            Genes (RNA)                       :: 94
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 127
            CDSs (without protein)            :: 127
            Pseudo Genes (ambiguous residues) :: 0 of 127
            Pseudo Genes (frameshifted)       :: 50 of 127
            Pseudo Genes (incomplete)         :: 102 of 127
            Pseudo Genes (internal stop)      :: 19 of 127
            Pseudo Genes (multiple problems)  :: 37 of 127
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP034557.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3849
                     /organism="Burkholderia cepacia ATCC 25416"
                     /mol_type="genomic DNA"
                     /strain="ATCC 25416"
                     /culture_collection="ATCC:25416"
                     /type_material="type strain of Burkholderia cepacia"
                     /db_xref="taxon:983594"
                     /plasmid="unnamed2"
     gene            205..531
                     /locus_tag="EJ998_RS40575"
                     /old_locus_tag="EJ998_40575"
     CDS             205..531
                     /locus_tag="EJ998_RS40575"
                     /old_locus_tag="EJ998_40575"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032431019.1"
                     /translation="MFGFFKKKKLYEEICKDAGMALSDGLLAQGLARNKIEAMGAGAVF
                     SQSLREAVSQGYKSSDAIAEARKNTSHHLAARGFDFETIASAIDVFCTATAFESMLDLA
                     RDKG"
                     /name="hypothetical protein"
     gene            complement(657..1121)
                     /locus_tag="EJ998_RS40580"
                     /old_locus_tag="EJ998_40580"
     CDS             complement(657..1121)
                     /locus_tag="EJ998_RS40580"
                     /old_locus_tag="EJ998_40580"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_061583928.1"
                     /translation="MEDKNNLSSEVKNHVSKWGKTNISAGWTIIPNALLENQSRLGLSC
                     IDTMVLINLIMHWWEKDNPPRPSKKRLANMLGVSLKTVQRSFIHLEQCGAIKRIPRYKE
                     GKDNARTTNHYDLNGLVDLLEGFSKELIEEREANRKSEVNRPKKRGNPKS"
                     /name="hypothetical protein"
     gene            1403..1969
                     /locus_tag="EJ998_RS40585"
                     /old_locus_tag="EJ998_40585"
     CDS             1403..1969
                     /locus_tag="EJ998_RS40585"
                     /old_locus_tag="EJ998_40585"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_061583929.1"
                     /translation="MAVFRQCEIREKYLAIYKESFDEVITYLEKAGALEPGVLRLTTID
                     DNAIRAWKSQWKGRSRKHAHGAWDWQNLVSKRARSCKRFDVAVWGEDVLCGLSVGKLTR
                     GKKTVRMDYLEACPTAHPLEKRITMIVVAVALSVAKKVGAQHVAIFNPIKDKEEKVLKH
                     YQSYGFTQRMLYGRFLKNVLYKEVV"
                     /name="hypothetical protein"
     gene            complement(1973..2143)
                     /locus_tag="EJ998_RS41040"
     CDS             complement(1973..2143)
                     /locus_tag="EJ998_RS41040"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_152924371.1"
                     /translation="MSYVSERKSELVKAQQRKMDPSSPDSAPINQGLMDFILLAMKVDD
                     GFVQRGRIHGA"
                     /name="hypothetical protein"
     gene            complement(2416..2560)
                     /locus_tag="EJ998_RS41590"
                     /old_locus_tag="EJ998_40590"
                     /pseudo=""
     CDS             complement(2416..2560)
                     /locus_tag="EJ998_RS41590"
                     /old_locus_tag="EJ998_40590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001717510.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            3104..3304
                     /locus_tag="EJ998_RS40600"
                     /old_locus_tag="EJ998_40600"
     CDS             3104..3304
                     /locus_tag="EJ998_RS40600"
                     /old_locus_tag="EJ998_40600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001558245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin VbhA family protein"
                     /protein_id="WP_061585996.1"
                     /translation="MTDDRTLQLRLTGFRKAEASLRLEGMDPSGTPLYESVKTRILSGD
                     ITFDEGRTEIRAHYQKRANSN"
                     /name="antitoxin VbhA family protein"
     Typing          complement(2453..3053)
                     /name="rep_cluster_2324"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.884"
                     /coverage="92.0"
                     /evalue="0.0"
ORIGIN
        1 gcgaaggcgc gcgtcattca ctctcgtgca tagccatgca gcggcctaaa aatcatcagg
       61 ggcgtttttc aggagtatgg acagggaaag gtgccggaaa cggcccggac gccccaggat
      121 cgcgctgagg ggctctcagg gggtgcgggc aagggatgtt atggtaaatt gctcatcaca
      181 gtcgccagtt aagcagaggt tagaatgttt gggtttttta agaaaaaaaa gctctatgaa
      241 gagatctgta aagatgcagg gatggcttta tctgatgggt tattggccca aggcttagcg
      301 agaaacaaaa tagaagcaat gggggctggt gctgttttta gtcagtcttt gagggaggct
      361 gtttctcaag gttacaagtc ttctgatgcg attgctgaag caagaaaaaa cacatctcat
      421 catctagctg ctagagggtt tgattttgaa acgatcgcaa gtgcgattga tgttttttgt
      481 actgctacag catttgaatc aatgttagat cttgctcgcg ataaaggctg aatatcgaga
      541 ggagtgacgg gttgttatca ttagcctcag agtagatctg aggccttttg ggcggtgtat
      601 gcaggagatg ttatggtaaa ttggactctc tacaggagag tgtgtttgtc agcctattaa
      661 cttttcggat taccgcgctt cttaggtcta ttaacttcac tttttcgatt cgcctctctt
      721 tcttcgataa gctctttcga aaaaccttcc agcaggtcta ccagtccgtt cagatcgtaa
      781 tggtttgttg ttctggcatt gtctttccct tctttgtatc gaggtatccg cttgatggca
      841 ccacactgtt ccaaatgaat aaaacttctt tgaacggttt ttagtgaaac gcccagcatg
      901 ttcgccagtc tctttttcga aggtcttggg gggttatctt tttcccacca atgcattatg
      961 aggtttatga gaaccatcgt atcaatacag ctaagaccca acctggactg attttccagt
     1021 aaggcatttg ggataatcgt ccatcctgca gaaatgtttg tcttccccca cttgcttacg
     1081 tggttcttta cttcgctcga caggttattt ttgtcttcca taaaacatgc atcctgtggt
     1141 catctgaggc tagagggcat tttatatatt gaacgtttta ccgttcacct tgcgtcattg
     1201 tcttaacgta ttgtcccact atatcttttt ctgtgtggtc aaatcagacc gtaccctatg
     1261 gtttttaaag tccacagggt ggggtttaaa tgtaccatag gatatggtct ttgttgacca
     1321 ctaaccaaaa tgttagcaaa tgatttaaag tggttttttt atggcaaaat gtttactggt
     1381 caaggtcagg agggagacga gcgtggcggt ttttagacaa tgcgaaatta gagaaaaata
     1441 tctcgcgatt tacaaagagt cattcgatga agttataact tatcttgaga aggcaggtgc
     1501 tttagagccc ggcgttttaa ggctcactac aatcgatgac aatgctattc gtgcatggaa
     1561 gagccaatgg aaaggcaggt ctcgtaagca cgctcatggt gcttgggatt ggcagaacct
     1621 tgtatcaaag agagctcgtt cttgtaaaag atttgatgtg gctgtttggg gagaggatgt
     1681 gctttgtggg ctttcagttg gaaagctaac ccgtggtaaa aagacagtta gaatggatta
     1741 tctagaggct tgtccaacag ctcatccgtt ggagaaacga atcacaatga ttgtggttgc
     1801 tgtggctctg tcagtagcta aaaaagtagg ggcacagcac gtagccatat tcaacccgat
     1861 aaaagacaaa gaagaaaaag tgttaaaaca ctaccagtct tacgggttta cacaaagaat
     1921 gttgtatgga cgctttttga aaaacgtgct gtacaaagaa gttgtttgat agctatgcgc
     1981 cgtgaatacg accacgttga acaaagccgt catctacttt catagcaagc aggatgaagt
     2041 ccataagacc ctgattgatt ggagctgaat caggagagga tggatccatt ttgcgttgct
     2101 gtgcctttac cagctcactt ttgcgctcag atacataact catcttgtga ctctctgtct
     2161 tgtatgggga tgtagacact ataacaagtg gataaaaaaa taacgagatc tctatatttt
     2221 caaaaagaga tgactgttgt tgactcgcct cttctgcaaa gcgtactctt ttctttcaaa
     2281 agaaaaaaac cgcctttgcg gggcggtttt tttccagttc attgagtttg cggctctttg
     2341 aacctaggta acagtcttgt gcgagacgtg tcaccaaatc tgtccttcca gtgtagctgt
     2401 agtcaggctt taacttcaag aactctcgat acacctctcg cactcactgc taccaatggc
     2461 taccgccagt ggcgctttgt cgtgtctttc agggttggat tcaagagtac agttacctga
     2521 taaagacgtg gtagtcgggc tgaacgggga gttcgtgcat acagcccagc ttggagcgaa
     2581 ccacctacaa ggaacccagt tgcgcgggta ggaatgagaa aacgccacct tacggcggac
     2641 tgcaacgggt aaacctcagg gtgggaacag gagagcgcac gagggagcga cccatcggaa
     2701 acggtgggga tctttaagtc ctgtcaggtt tcgccacttc tgatttgagc gccggaaatc
     2761 acctgatttc tgtgaagctt gtcaggaggg cgaagcctat ggaaaaccgg caatccccag
     2821 aacctcctta tggccttctg cctgagcagg taacgctcgc cgcagtgtca tgaccgaacg
     2881 aagtgagcga atgaccgagg aagcgtaatg tctgtaatgc tgaacaggga tcgtgtcgct
     2941 cggtacacac gtttcctccc tgattacaca cccggctgca acagtttcct gtcgttttcc
     3001 acttttccct gcacagctat acaacggcaa catccgggca ggacatgtaa agttagctaa
     3061 ctttacgcct gccttattcg catgactgct caaaggaaaa accatgactg atgatcggac
     3121 actgcaactg cgcctgacag ggttccgtaa agcggaggcc tctctcaggc ttgaagggat
     3181 ggatccatca ggaaccccgc tttatgaatc ggtaaagaca aggattcttt ccggtgacat
     3241 caccttcgat gaaggccgca ctgagatacg ggctcattac cagaagcggg cgaacagtaa
     3301 ctgacgcgga caggaaatgt tttgtggcca ccgccgctgc gccggcgcca caaaatggcg
     3361 ggacaagatg tgtttttgga gtaccgccgg cactccagaa tgtttcatca ggccccgccc
     3421 gataccctga atttcgtacg tacgaaattt cgctgctgtt cagatcgaga gcaggattcg
     3481 caaattgctg gcgtttgtac ccactgtaaa tctgccgcgc ctggcggctt ggccaacgct
     3541 tcacgaacgc acagtaaact gcgcactcgt tcaccgttac cagaaaggcc attgcgggaa
     3601 taattctttc agtcgttcta cgagatcaag cagctcattc atgaccttca gcatgacgct
     3661 tatcagtttc aaaaatttag gcatataaaa atttcctgta cggagttggg tctggatcag
     3721 gaaattaggg actttgtaat tttcagcggt agaaaaatca gtttttctaa aaaaattttt
     3781 taggcggcga aaagtgaagt aatgagcact gcttcgtgct gaaaatgagg ctcaggcagg
     3841 tgttttgca
//
