LOCUS       NZ_CP034418            10061 bp    DNA     circular CON 26-NOV-2023
DEFINITION  Klebsiella pneumoniae strain C789 plasmid unnamed1, complete
            sequence.
ACCESSION   NZ_CP034418
VERSION     NZ_CP034418.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN10537194
            Assembly: GCF_009684575.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10061)
  AUTHORS   Zhang,Y., Jin,L., Ouyang,P., Wang,Q., Wang,R., Wang,J., Gao,H.,
            Wang,X. and Wang,H.
  CONSRTM   China Carbapenem-Resistant Enterobacteriaceae (CRE) Network
  TITLE     Evolution of hypervirulence in carbapenem-resistant Klebsiella
            pneumoniae in China: a multicentre, molecular epidemiological
            analysis
  JOURNAL   J Antimicrob Chemother 75 (2), 327-336 (2020)
   PUBMED   31713615
REFERENCE   2  (bases 1 to 10061)
  AUTHORS   Zhang,Y., Jin,L., Ouyang,P., Wang,Q., Wang,R., Wang,J., Gao,H.,
            Wang,X. and Wang,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-DEC-2018) Department of Clinical Laboratory, Peking
            University People's Hospital, No. 11 Xizhimen South Street, Beijing
            100044, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Other Unicycler pipeline v. v0.4.6
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 125.0x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/26/2023 04:02:12
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,864
            CDSs (total)                      :: 5,742
            Genes (coding)                    :: 5,578
            CDSs (with protein)               :: 5,578
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 164
            CDSs (without protein)            :: 164
            Pseudo Genes (ambiguous residues) :: 0 of 164
            Pseudo Genes (frameshifted)       :: 68 of 164
            Pseudo Genes (incomplete)         :: 106 of 164
            Pseudo Genes (internal stop)      :: 22 of 164
            Pseudo Genes (multiple problems)  :: 32 of 164
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP034418.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10061
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="C789"
                     /isolation_source="blood specimen"
                     /db_xref="taxon:573"
                     /plasmid="unnamed1"
                     /geo_loc_name="China: Shandong"
                     /collection_date="2016-04-14"
     gene            join(9635..10061,1..1259)
                     /locus_tag="EJJ25_RS28610"
                     /old_locus_tag="EJJ25_29910"
     CDS             join(9635..10061,1..1259)
                     /locus_tag="EJJ25_RS28610"
                     /old_locus_tag="EJJ25_29910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            1269..1526
                     /locus_tag="EJJ25_RS28615"
                     /old_locus_tag="EJJ25_29915"
     CDS             1269..1526
                     /locus_tag="EJJ25_RS28615"
                     /old_locus_tag="EJJ25_29915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            1611..1760
                     /locus_tag="EJJ25_RS28620"
                     /old_locus_tag="EJJ25_29920"
     CDS             1611..1760
                     /locus_tag="EJJ25_RS28620"
                     /old_locus_tag="EJJ25_29920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(1975..2124)
                     /locus_tag="EJJ25_RS30855"
     CDS             complement(1975..2124)
                     /locus_tag="EJJ25_RS30855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_319922287.1"
                     /translation="MGCKQELPVQPDCCALSGNCSLESNPGKHAKTPLAAAIGNWEFAE
                     DLFS"
                     /name="replication initiation protein"
     gene            3245..5209
                     /locus_tag="EJJ25_RS28625"
                     /old_locus_tag="EJJ25_29930"
     CDS             3245..5209
                     /locus_tag="EJJ25_RS28625"
                     /old_locus_tag="EJJ25_29930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            5209..5940
                     /gene="mobC"
                     /locus_tag="EJJ25_RS28630"
                     /old_locus_tag="EJJ25_29935"
     CDS             5209..5940
                     /gene="mobC"
                     /locus_tag="EJJ25_RS28630"
                     /old_locus_tag="EJJ25_29935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            5947..6477
                     /locus_tag="EJJ25_RS28635"
                     /old_locus_tag="EJJ25_29940"
     CDS             5947..6477
                     /locus_tag="EJJ25_RS28635"
                     /old_locus_tag="EJJ25_29940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(6504..6683)
                     /locus_tag="EJJ25_RS28640"
                     /old_locus_tag="EJJ25_29945"
     CDS             complement(6504..6683)
                     /locus_tag="EJJ25_RS28640"
                     /old_locus_tag="EJJ25_29945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(6709..7137)
                     /locus_tag="EJJ25_RS28645"
                     /old_locus_tag="EJJ25_29950"
     CDS             complement(6709..7137)
                     /locus_tag="EJJ25_RS28645"
                     /old_locus_tag="EJJ25_29950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(7301..7615)
                     /locus_tag="EJJ25_RS28650"
                     /old_locus_tag="EJJ25_29955"
     CDS             complement(7301..7615)
                     /locus_tag="EJJ25_RS28650"
                     /old_locus_tag="EJJ25_29955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(7603..7881)
                     /locus_tag="EJJ25_RS28655"
                     /old_locus_tag="EJJ25_29960"
     CDS             complement(7603..7881)
                     /locus_tag="EJJ25_RS28655"
                     /old_locus_tag="EJJ25_29960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(8056..8421)
                     /locus_tag="EJJ25_RS30655"
                     /old_locus_tag="EJJ25_29965"
     CDS             complement(8056..8421)
                     /locus_tag="EJJ25_RS30655"
                     /old_locus_tag="EJJ25_29965"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            complement(8418..8789)
                     /gene="tolA"
                     /locus_tag="EJJ25_RS28665"
                     /old_locus_tag="EJJ25_29970"
     CDS             complement(8418..8789)
                     /gene="tolA"
                     /locus_tag="EJJ25_RS28665"
                     /old_locus_tag="EJJ25_29970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(9063..9308)
                     /locus_tag="EJJ25_RS28670"
                     /old_locus_tag="EJJ25_29975"
     CDS             complement(9063..9308)
                     /locus_tag="EJJ25_RS28670"
                     /old_locus_tag="EJJ25_29975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     BGC             1..10061
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(1864..2443)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          5210..5937
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 ttgcgatcta cggtgtgctg ccttctgaga ttgcaaaaga tgatccgaaa atgatgtcaa
       61 aaattatgac gtcattaccg gccgatgcgg tgacggagac tccggcaagt actttaccac
      121 tggaccaggc gacggttcgt gtcagacaac gggttgtgga tgtggtgaag gatgagcggc
      181 agcatattgc ggttgtcgca ggtcggccaa tgagtgtccc tgtggtggat gcgaaaccga
      241 caaaacgtcc gggggtattc agtgtgtcga ttccgggtct cccgtctctg caggtgagcg
      301 taccgaaagg tgttccgaca gcgaaagccc cgccaaaagg cattgttgct gaaaaaggtg
      361 attcacgtcc ggctggtttt acagccggtg gtaactcccg tgaggccgtt attcgtttcc
      421 cgaaagagac cggacagaag ccggtttatg tgtcggtgac agatgttctt accccggcac
      481 aggtaaaaca gcgtcaggag gaagaaaagc gtcgccagca ggcatgggac gccgctcatc
      541 cggaagaggg gctgaaaaga gactatgata aagcgaaagc tgagctggat gccgaagata
      601 aaaatattgc gaccttaaac agccgcattg catcgacaga gaaggcgctc cccggtgcaa
      661 gggctgctgt tcaggaagcc gataaaaagg taaaagaggc agaggcaaat aaagatgatt
      721 ttgtgactta taacccgcct catgaatatg gctccgggtg gcaggatcag gttcgctatc
      781 ttgataagga tattcagaat cagaatgaga aattaaaagc ggctcaggca tctttaaacg
      841 caatgaatga atccttatcc agagataagg ctgcacttac cggggcgatg gagagccgga
      901 aacaaaagga gaaaaaagcg aaggatgcag aaaataagtt aaatgaggaa aagaacaagc
      961 ctcgcaaggg gactaaagac tacggccatg attatcatcc agtacctaaa acagaagaca
     1021 taaagggact tggagagtta aagcgaggtg atcctaaaac acctaaacaa ggtggtggtg
     1081 gtaagcgggc ccgatggtat ggcgataaaa aacgtaaaat ttatgaatgg gattcccagc
     1141 acggtgagct tgaagggtat cgtgccagtg atggcgaaca cctaggcgca tttgatccca
     1201 aaacgggtaa gcaggttaaa gggccagatc caaaacggaa cattaaaaaa tatctttgag
     1261 aggtaaatat ggggcttaaa ttacgtttga catggtttga taagaaaact gaagagttta
     1321 aaggggaaga gtattctaaa gactttggtg acgatggttc tgtcattgaa agcttgggaa
     1381 tgcctttaaa ggataatatt aataatggtg agtttgattt gaagaaagaa tgggttcctt
     1441 tactacagcc attttttaaa aatcaaataa aacctgatgt ggataattgt tatatagctt
     1501 ttgattatcg ggacggtaac tggtaacaaa aaattatcaa ggatgatttg aaatactgac
     1561 ttccggtgtt cagggatgaa tgccggagtc tttaaataca gggaaagtat atgaaaaagg
     1621 taaagactat ttttcttttt atattgattg ttgctggctt tcttctggcc gcatgtcagg
     1681 caaattatat ccgggatgtt cagggcggaa cggtggcacc atcgtcctcc tctgaactga
     1741 cggggatcgc ggttcagtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     1801 cgtaatcttt tgccctgtaa acgaaaaaac cacctgggga ggtggtttga tcgaaggtta
     1861 agtcagttgg ggaactgctt aaccgtggta actggctttc gcagagcaca gcaaccaaat
     1921 ctgtccttcc agtgtagccg gactttggcg cacacttcaa gagcaaccgc gtgtttagct
     1981 aaacaaatcc tctgcgaact cccagttacc aatggctgct gccagtggcg tttttgcgtg
     2041 ctttcccggg ttggactcaa gtgaacagtt accggataag gcgcagcagt cgggctgaac
     2101 ggggagttct tgcttacagc ccagcttgga gcgaacgacc tacaccgagc cgagatacca
     2161 gtgtgtgagc tatgagaaag cgccacactt cccgcaaggg agaacggcgg acaggtatcc
     2221 ggtaaacggc agggtcggaa caggagagcg caagagggag cgacctaccg gaaacggtgg
     2281 ggatctttaa gtcctgtcgg gtttcgcccg tactgtcaga ttcatggttg agcctcacgg
     2341 ctcccacaga tgcaccgaaa aagcgtctgt ttatgtgaac tctggcagga gggcggagcc
     2401 tatggaaaaa cgccaccggc gcggccctgc cgttttgcct cacatgttag tcccctgctt
     2461 atccacggaa tctgtgggta actttgtatg tgtccgcagc gcccgccgca gtctcacgcc
     2521 cggagcgtag cgaccgagtg agcgaggagg cgcaatttcc ccggcacctg caagacggtc
     2581 accggacgcg ctgatgtggg atattgtccc ctgattaagt ctttctattc cctgacgatc
     2641 aattatcagg ataggtgaac catgtcggtt tttcgctgaa cattttacgt taacctatct
     2701 ctgttgcttt tttctccttg cggagaaatg caagtcatcc ccgctggggc gtggctgact
     2761 tcaggggaaa agaccttgct tacgctggct ggacgccacc gacgcacggt cttttcccct
     2821 ctttctggct ctgtccggct ggacgccggc cacgccagaa attcacccgg cccccgtcag
     2881 gctcacgcct gaccacccac aaaaatccac ggtgctggac gcacctgacg aagcagcaaa
     2941 gctgctcctg attttgcggg tggtcagccg ctcgctatgg cgaactctga cgggggccgg
     3001 aacaccggaa aagaccgcac tggggtgccg ccttttcctc tcacacgctg gggcgtgctc
     3061 gttgttcctc cggggctgcg ccttttccgc atggggcgga aagaggctgg cctccgcggt
     3121 gaaagctgaa cgcaatcacc acggacgtga ccgccgccgc catgccttgg ggcatgtccg
     3181 cggcttgtgc agcgggggct gcgcatcacg ccggtaaacc cgtcgtctga aggagagaga
     3241 ttgcatgttt aatacggatt cgcttgcctg gcagtggcat atcccggagc tggcccggca
     3301 gatttacccg tgggtcagct ggctgtaccg ggagtattac agtctggccg gtttttttct
     3361 ggttatcggc ctgtttttcg gtctggctga actctgggcc ggccgaatgc gttttgtggg
     3421 gctgagccat aaggcgcggc gggagtatgc cgatcgctat aagcgcgata ataaccggac
     3481 tgtgccattt tcggtgctgg cctcgctgga ttcacccggg cggatggtca gggcacggcg
     3541 acatgtctgg aactacgcgt actggctgac cggcgctgcc ggtctggccc ttctgggggg
     3601 gctggttgtc gccgtgttta tcgagagctg gggagaaacc gtcctgcgcc cggacctcta
     3661 tgcgaacgca gattattacc tgctgcagcc ggctaacctg ttcgggatag cctggtgtgc
     3721 cggagggctt gtcgccggcg tccttctggc gcggctgctg gcgttcctgc tgcttgatgg
     3781 tcattttgcg gccgctgacg aggcggtgaa cgcgaaactg aatcaggaga gccggcgcag
     3841 cagccagcgg accggagaaa tgacagacgt ccgccacctg catttcgggg agccggtacc
     3901 ggttaacgcg ctggccgatt tttccacgga gcaggcccgg aaacagcagg cggtgtttct
     3961 gggtaaggat gagcagggcc agccggtgct ggtccctcgt gatacctggc gcaagaccaa
     4021 tattcagatc ctcggcctgc ccgggagcgg caagagcgcg atggccacca acgcgctcat
     4081 tcgctgcgta cgcgatttcg gggacgctgt ggtgtacttc gaccccaaag gggacgcgtg
     4141 ggcgccgcac gtcttccggg cgcactgccc ggacttcacc ctgctggatc tgcggccggg
     4201 gaaaccggcg cagctgaatc tgttccgcga tctcgaccag tacgccctga agaatctgct
     4261 ggttgccggg tttaacctga gtgagaccgg cgacgtggcg gaccactacc gcatcagcga
     4321 gcagaaagcg gcgaagctga ttgcggagca gttcccgcag ggggcaaaca ttcagcaggt
     4381 gctggcggcg gcgtacgcgt tgccggaggc cctgaaaaag gacgtgaagg ggctgatcac
     4441 caagctggag aacgtggccg acctgagcgt cctgcagacg gacagcggta tcgatgtggc
     4501 ggggataatc aacggcggcg gctgcctgta cgtcatcggc tcgatggacg atgaggcggt
     4561 gatccgggtg cagaagatgc tgtttgcccg ctgcgcgcaa atcatcattg cgcgggatga
     4621 gttccggagg tggccgcacg cgagcatcat gctcgatgag attaaatatc tgctgtcgaa
     4681 gtacgtgctg aacgcgctgg ggacgctgcg cagccgggac tgcaacctgc tgctcgcgca
     4741 ccagtcgctg ggcgacttcg gtcagtgcgg gcaggatctg cccgctgatt ttgtgaaaac
     4801 cacggtactg gataacacgc cgatccgctg gttctatcgc gcggccagcc aggagtccgc
     4861 gcagtgggcg gcgggccaga ccggggagat ccgggtggac gtggagcgcc gccgggccag
     4921 ccgggaggcg gggaacgtgg agcacatcag cggcgacagc ttcatccaga aggaggcgcg
     4981 gccgctgttt gacgtgaaca ccctgcaaca cctgcctgac gggttcgcgg tgatgaccgg
     5041 gctgggcgtg gcgcggctgg cgttcagcag cccgctgcgg gttgagcgcc gggaaatttc
     5101 gctgaaaacc ttcccggtgc tggcgaaaac ggacccgctg gcggagtacc tgcctccggc
     5161 cgacggcggc ggaccaaaag cggatgatga ttttgcgggg ctttactgat gacactggag
     5221 cagtacaacg tcagccacgc gaagcggcag gcgcggaacg cggagaaaac acggctgacc
     5281 ctgcgctggc tgcgggaaga gctgtgttcc acggcggagc tggttgcgcg acggctgggg
     5341 attgcggcgg tacagccggt ttaccgcttc ctggacagtc tggtggcgaa agggctgctg
     5401 gtccgggcga aatacccggt tgacggccgt caggtcagcg tgtgggggct gacgccgcac
     5461 ggggtggcgt tctcgttcga cgaggacgag ccgctgacgg acgttattcc gtttcagccg
     5521 tcaagggtgt cggcggcgca gctgccgcac cggctggccg tgcagtccct gcgcctctcc
     5581 atggagggcc ggggggccac cggctggcgg tacctgcacc gtatggccct gaaagggatg
     5641 aaagtgccgg acgcgctggc ggagctggac ggcagaacgg tggcatttga ggtagagcgc
     5701 accgtcaaga gccgcaggcg gtatcaggaa gtggtgtccg ggtatctgtt caaccgccgg
     5761 gcgaacggca tcgatgaaat ctggtacatc tgccctgacc ggccgacgct tgtccgggta
     5821 cagcgggcga ttctggcggt ggatgaaatc gtgaatccac agaccggcga ggcccgcaaa
     5881 acggtggagc tggaccggga gaggctgttc gcgtgcttta aatttatgac aacggagtga
     5941 gggattatgg cgctgtacat ggtatttatt gaggacgtgg aagacaaaga cggcgagcgc
     6001 tgcattatgc caaccagcga gcatctggaa gacatggtgg tgctcgcctg gcagaccggg
     6061 gcgtatgttt tctgttttgt cgaccgggcc cagcatctgg cgatgatgaa atccatcggg
     6121 atgtatgacg agcggctcaa cagcgacgaa aaagccacgt actttttcac cggtgaaaat
     6181 ccgcaatggg aacgggacaa acccacagga atgaagctgt gtgattacct ctatcgaaag
     6241 gtggagcggt tcgccatcga gaacggtgtg caggtggttg atgccgggaa gtgctcaccg
     6301 gagcagatta acgcgatgat tgaggtggtc agcgagcggc acggggaaag cgcggtgaac
     6361 gagaagctga aagcggagct gatgcgggaa tataacagcc gctattttaa cgtgccggtg
     6421 cctgaactgc cggaggacaa acccgcccgc cggtggtggc cgttcggcaa aaaataacgc
     6481 cagcaccggc atggccggtg ctgttattct tccccgagct tatcccgcat cacctgccag
     6541 agtgtttccg catcctcgtg aagccgttcg cacatgtcgg ccggggtatc catgtccagc
     6601 tcgttcagct tttccagcaa cagcagcgac tgatccttaa tgaatttagc catattgagg
     6661 gcagtttttt cctgcttcgt catgttctcc attcccttcg gtaagttctc agctaaacca
     6721 gccggaccag aagcgttttt tcttctgtgt cagttctgtt gtcaatctgt ccacttcggc
     6781 ctggagtttc cggcgctcat catcgtgctg gcggcgttct tcttcccggg atgttttatc
     6841 ttccagcatc agggtgacag cctgccggag atcggccagt tccttttgca ttgccagaag
     6901 ctgttcctgt gggaaatttt tttgtgtatt ttcacggtgt gaaatgccgg gaatttgttt
     6961 ctcagggata ttcacatttc cgtatacacg gatcagttct gacacatcca cgacagggtt
     7021 gtttttcccg tcacgtgaca cggtaacctt tccctgtttt acatgattgt atagcgttct
     7081 tctggtaatg cccgcggctt gtgctgcctg tgaaaggttg agcaatgttt tcgccactgt
     7141 atacccctga ttttatcggg tgtgaaaggt gtgtaatttc tcacggtaaa tgtatcgctg
     7201 aattttaccc gtttttccgt tcaggatgtg gttttctgac gcgctgcgct tgtcaaaccg
     7261 gcgacggcca cgcaatgaat tgcgcccccg tcatcggtta ttagaggctg tctgcccccg
     7321 gatacccgga ttctttctga tgcctgacgg tgacgatcac aatctggtcc atttcccggt
     7381 cgtggcggta gagcatcaca tagccactgt cgccaaaccc gatcaccagt tccttatatt
     7441 ccagcggcag aaacggcacc gggcggccaa tgtctggcaa tgttttcagt tgctggatag
     7501 cccggacgat cacctcacct ccttttctgg ctgccagccg atttttggtt ttgagaaagt
     7561 cctgaaggcg ttgtaaatcg cgctgtgcca gcgcggaaat cgttacctgt ggcatggcgg
     7621 agcatcctgc tcgttctcag ttccccaggt acttatccag gcttccgctt cttccgcagt
     7681 aaggtgcaga cccgtttcct gatagtgctg ccatgcggcc tgtccgtccc gcaggtactg
     7741 gctgcgcttc tcttcccggt cgatgtattc ggtgattgcc tccagcatta gcgcgtgcgc
     7801 tgagcggtgc cggtcatcgg ccagcgtttt cagccggtcc ttaagttctt catcgagcct
     7861 gatggatgtt gctgatgaca taaaagcccc tttgtagtca tatgtattac acatgactac
     7921 tttaatccgt ggcggcgcta tttgccagaa atacggcttt tgctgcccat ttttcagctt
     7981 cgcttccact aatcagggct gctacaggct cactcatcat ccaggactat tgcagcctgc
     8041 gggttaaagg acctgttact ggcggcaacc atcatcattg agcttttggg tggtgactgg
     8101 tcgcggatcc tgtaagccgt catttttcag actgaattca aaatttataa cctgtcgcat
     8161 tgccgggtgc cctgcgggat atagccagtg cctcattgcc gtcagcgttt cccgaaaaaa
     8221 aaggtgacag ccggtaagcg acacgttgtc cactcgtcca tcgtccagaa tatcaaacgt
     8281 ggcggtcacc tgaccttcga tgcgttttgc tgcggcctgt accgggtagg ttggctgcag
     8341 tgtggttacg ggttttagtg gtatcgcatg taccggagtg aggaacatgg ccgccagcac
     8401 tgtcagagta aaagctttca tggtctgaaa tccagagggg cgtttttgaa tatttgccag
     8461 gtattttcat ccggtgccgg cggaattttg gcgtgggtaa cggctgatat tgctgcttta
     8521 cagagtttcg gatctcctcc ttcagcaatg gcgctgttca gagtgccatc acgttgtatg
     8581 gatatccgca cggtgcacat ttttcccgca taggcctctg gttcaaacat ctgcatttca
     8641 acagcggtct tgatggccgc agcgtagcta ctgattttag atgacttatc cagagtataa
     8701 attttcggcg gctgggtggt ggaatgctta caggccgtca ggagtaaggg gctgcacaat
     8761 gccagcagga taaagggtat taaacgcatc gggttgatga cctgtcagta aaaaatgtta
     8821 catgtatatt tgtcactctt tacggccacg ttcatttaca taaacacggc ttatcgtatc
     8881 gtttttacac caaacggccc gccatgcagt tgcgtgcata ggcatgcagc gggttacaat
     8941 ttaccgggcg cgtttccggc ggttttccgg gtggtttgtt gcctgtttta tcccgtagcc
     9001 gccggaaacg ccctcagacc gtctgagcgg tgcgggcaat gcgacgttat ggtaaatacc
     9061 ccttaccggc ggcgctgtgg ctgccaggtg aaggtggttt ccgtagcctc gtatttctcc
     9121 cgacgcattt cctgcgcccg cctggcttca cgttcgtcct gaatggcacg aagcaccctt
     9181 tcccgcacct gttgttttac cgcctttggc agcgttttgt tccctttcag gcccatggcc
     9241 agtgcctgag cgacaaaggc gtccgccagg cgcttttctt cagcgggagt cagtttcagg
     9301 tgcgtcatag tggcctccgg gcgtctcagg tttcccgaac agtttaaccg caggtcctct
     9361 cctcatgcca gttagctttc agatgtgtct ataccatctg aaatgtaagt gtgatgaatg
     9421 tcttaaataa taaaatttgt tgccatgaga aattttcggg aaaagacgcg atctgcttac
     9481 aggtggctcc gggcgctttt ttgtgcctga agatgagaac taatctcata tattgacacc
     9541 tgaaaacggg aggagtaagg taataattat actgtgtata tgtacagtat gttggcttta
     9601 atttattgtt ttaaatgtca aagaggaaaa cggtatgagt ggtggagacg gtcgaggtcc
     9661 gggtaattca ggtctgggac ataatggtgg tcaggccagt gggaatgtga acggtacgtc
     9721 tggtaaaggt ggcccttcat caggtggtgg tacggatcca aacagcgggc cgggctgggg
     9781 tacgacgcat acgccgaatg gagatattca taactataat ccgggggagt ttggtaacgg
     9841 cgggagtaaa cccggtggta atggtggtaa cagcggcaat catagcggta gctctggtgg
     9901 cggacagtct tcggccactg cgatggcctt cggtctgcct gccctggcta ctccgggggc
     9961 tgaaggactg gctttatcag tttccggcga tgcgttgtcg gccgccgttg ctgatgtgct
    10021 ggctgccctg aaggggccgt ttaagtttgg tctgtggggg a
//
