LOCUS       NZ_CP034387             4091 bp    DNA     circular CON 25-JUN-2024
DEFINITION  Escherichia coli O157:H7 strain FRIK804 plasmid pFRIK804-02,
            complete sequence.
ACCESSION   NZ_CP034387
VERSION     NZ_CP034387.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN10531264
            Assembly: GCF_009931235.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli O157:H7
  ORGANISM  Escherichia coli O157:H7
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4091)
  AUTHORS   Stanton,E., Wahlig,T., Park,D.-J. and Kaspar,C.W.
  TITLE     Escherichia coli O157:H7 strain FRIK804
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4091)
  AUTHORS   Stanton,E., Wahlig,T., Park,D.-J. and Kaspar,C.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-DEC-2018) Bacteriology, University of
            Wisconsin-Madison, 1550 Linden Dr., Madison, WI 53706, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 16-MAY-2018
            Assembly Method                   :: Canu v. 1.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 36.51x
            Sequencing Technology             :: PacBio RSII; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009931235.1-RS_2024_06_25
            Annotation Date                   :: 06/25/2024 01:58:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,666
            CDSs (total)                      :: 5,536
            Genes (coding)                    :: 5,219
            CDSs (with protein)               :: 5,219
            Genes (RNA)                       :: 130
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 101
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 126 of 317
            Pseudo Genes (incomplete)         :: 175 of 317
            Pseudo Genes (internal stop)      :: 70 of 317
            Pseudo Genes (multiple problems)  :: 44 of 317
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP034387.1.
            Bacteria and source DNA available from Charles W. Kaspar.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4091
                     /organism="Escherichia coli O157:H7"
                     /mol_type="genomic DNA"
                     /strain="FRIK804"
                     /isolation_source="Calf"
                     /db_xref="taxon:83334"
                     /plasmid="pFRIK804-02"
                     /geo_loc_name="USA:Wisconsin"
                     /lat_lon="43 N 89 W"
                     /collection_date="1994-10-27"
                     /collected_by="Charles W. Kaspar"
     gene            complement(81..2228)
                     /gene="mobQ"
                     /locus_tag="EJB16_RS27960"
                     /old_locus_tag="EJB16_28925"
     CDS             complement(81..2228)
                     /gene="mobQ"
                     /locus_tag="EJB16_RS27960"
                     /old_locus_tag="EJB16_28925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_024236174.1"
                     /translation="MAIYHLSMKILSRSKGYSAVASAAYRAGEKILDERTGVIHDYTRK
                     NGVASAVILTPANAPAWCANRAELWNAVEKAERRKNSQLAREFELAIPRELAQDAARET
                     VLNFVRENFVSRGMIADVAFHNLGGSNPHAHIMLSMRAITPDGFGEKVREWNDWTHAET
                     WRGSWADHANRALANAGYQEEIDHRSYERQGVEKTPGIHLGKSACAMEKWGIETERGEQ
                     NRLINRLNLEIQVSRDELRNSELTEPARRMADLLNIELPDNATSYILHDIIEALPTDSN
                     AAWKLTSKAMSMMADMQATRRRWEDLNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWV
                     APEYGREQEKIQSLSQRMEKLRQHHWKVEKQDIPASQKAFETQWQQWGASGLADLREQL
                     RKREEELRRKEQEETERRRAEQLRRNDNAVMDSGVLKAVVTGYGEAPMPGNGEMTCYLL
                     LHNRNGEYTLWGNELEKYRARIFESVDLMRDRSGYICERSEIGQRPPLQHVYSSATFEQ
                     LLTQVCQTWPQHTRDLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLPPTLMPVM
                     SSVDRETRQLQLLLVMGVDDSMGGVVRLNGTLYPAFAVPTADNSQLVINALTDKGLRFA
                     GYGEAVNHDADSPARPAPELMQFHLKTWSEPLFAIVNTPEKQPDHLFRSLGFERTWDEW
                     QRDEYARTHATERRHDRGWSQ"
                     /name="mobQ"
     gene            2399..2689
                     /locus_tag="EJB16_RS27965"
                     /old_locus_tag="EJB16_28930"
     CDS             2399..2689
                     /locus_tag="EJB16_RS27965"
                     /old_locus_tag="EJB16_28930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="WP_137491003.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVIVGAALLAKVRKP
                     RETQLRAWLLHLLKTEVTRQADVSRIQPLIDELNALPKPVPKEVSENDQQA"
                     /name="mobilization protein"
     gene            3222..3923
                     /locus_tag="EJB16_RS27970"
                     /old_locus_tag="EJB16_28940"
     CDS             3222..3923
                     /locus_tag="EJB16_RS27970"
                     /old_locus_tag="EJB16_28940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_011666799.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCIGRLKSGSDAESTEL
                     QKTMTVTAEEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKDDEKREEFRWIQYRAQY
                     AKGEGKAQITFSDAVMPYLTQLKGQFTRVVIKNISNLSRSYSIRIYELLQQFRSTGERI
                     IALDDFRSMLDIEHKYQTYKSLNQQLLRPCVDELNQKSDLAVTLETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     Typing          1874..2228
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2245..2395
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="92.157"
                     /coverage="85.0"
                     /evalue="1.64e-53"
     Typing          3223..3922
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.301"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(85..2270)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.726"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tgtgcttctt gcattcatcc ctgtcgatga acatcgacga acataacacg atgttaaaca
       61 aaacaaaata ttggtcacat tcactgtgac caaccgcgat catgtctcct ctcggtggcg
      121 tgtgttcggg catattcatc acgttgccat tcatcccagg tgcgctcaaa gcccagcgag
      181 cggaacagat gatccggctg tttttctggc gtgttgacga tagcaaaaag cggttcgctc
      241 caggttttca gatgaaactg cataagttcc ggcgcaggac gtgccggact gtcagcatcg
      301 tggtttaccg cttcaccata gcctgcaaag cgtaaacctt tgtcggtcag tgcattgatt
      361 accagctggc tgttgtcggc tgtggggacg gcaaacgcgg gatacagcgt accattaagc
      421 cggacaacac cgcccataga gtcatccaca cccatgacca gcaaaagctg aagctggcgg
      481 gtttcacggt cgacagaact cattaccggc atcagagtcg gtggcagacg tccctgcgta
      541 aaatccacct tccttgccgc cagtgatggc atggctggct ggcggtcttc tccaagacag
      601 aaagactccg gccatgtctt cggctggcgg agatcgcggg tatgttgcgg ccatgtctgg
      661 caaacctgcg tcagcagttg ctcaaaggtc gcagaggaat aaacatgttg cagtggtgga
      721 cgttgaccga tttcgcttcg ctcacagata taaccactcc tgtcacgcat taaatcaaca
      781 ctctcaaaaa ttctggcgcg gtatttctcc agttcgttcc cccagagcgt atattctccg
      841 ttccggttgt gaagcagaag gtaacaggtc atttccccgt tacctggcat gggggcctcg
      901 ccataccccg taacgactgc tttcagtacc cctgaatcca taacggcatt gtcatttctc
      961 ctgagctgct ccgcccgacg acgttctgtt tcctcctgct ccttccggcg caattcctct
     1021 tcacgtttac gcaactgttc tcggagatct gcaaggccag atgcacccca ctgctgccac
     1081 tgtgtttcaa atgctttttg actcgcggga atgtcctgtt tctcaacctt ccagtgatgc
     1141 tgccgcaatt tttccatgcg ctggctcaac gactggattt tttcctgttc gcggccgtat
     1201 tccggtgcaa cccattgcat caggggaata cgggaaaaac cggacgaaaa cggcgatgat
     1261 tttttcaggc tggcagcatg gctcatcgct tcctttcgtt cggtgttcag atcttcccag
     1321 cgtctgcgcg tcgcctgcat atctgccatc atcgacatgg ctttactggt gagtttccat
     1381 gctgcgttac tgtccgtcgg caacgcttcg atgatgtcat gcagtatgta cgaagttgcg
     1441 ttgtcaggaa gctcaatatt cagaaggtca gccatacggc gtgcgggctc tgtcagctca
     1501 ctgttcctga gttcgtcgcg ggaaacctgt atttccaggt taagcctgtt aatcaggcga
     1561 ttctgttcgc cgcgttctgt ttcgatgccc cacttttcca tcgcacaggc cgactttccg
     1621 aggtgaatgc caggcgtttt ctcaaccccc tgtcgctcgt atgaacgatg gtctatctct
     1681 tcctgataac cggcgttcgc cagcgccctg ttcgcatggt ctgcccatga tccccgccat
     1741 gtttcggcgt gagtccagtc attccactca cggactttct ccccgaagcc gtcaggcgtg
     1801 attgccctca tggatagcat gatgtgcgcg tgtgggtttg acccgcccag attgtgaaag
     1861 gccacgtcgg cgatcatgcc gcgactgaca aagttttccc ttacgaagtt gagaactgtt
     1921 tcccgtgcgg catcctgcgc cagttcacgg gggatggcga gttcgaactc ccgcgcaagt
     1981 tgtgagtttt tccgccgctc ggctttttca acagcattcc agagttcggc gcggttcgca
     2041 caccatgctg gggcgtttgc tggcgtcaga atcactgcgc tggctacacc gtttttgcgg
     2101 gtgtagtcgt gaatcactcc ggtgcgctca tccagtattt tttctccggc acgataggca
     2161 gcagaagcaa cggcgctgta gcccttgctt ctggaaagaa tcttcatgct gagatgataa
     2221 attgccatcg tgtcgttgtt gccgttttct tttagccccg cagggcgcac acaaactcga
     2281 cgaaggagag tttgtataag tgcgcacttc gtgatttgct gtgcattatg ccagttcagt
     2341 ttttaaacta ccagtgacgc gctaaaatac gatttttcac cgcgcacgga gttacggaat
     2401 gagtagaaca ctcgaacaga agattgccga tgctgaggcc agattgcagc gtctgaaggc
     2461 gaagagcagg agcctggaca cagcgcagaa ggtcattgtg ggagcggcat tgctggcaaa
     2521 ggtcaggaag cctagggaaa cacaactacg agcctggttg cttcatctcc tgaagacaga
     2581 ggtaacacgg caggctgatg tgagcaggat acagccgttg attgacgaac tgaacgcgtt
     2641 accaaaacca gtccccaaag aagtgtcaga aaacgaccaa caggcgtgaa tgcaccgtcc
     2701 gctcgccgca gccccagcga ccgagcgtta gcgagcgagt gggcgaggaa gcggaagtga
     2761 atccgtcgcc agattgagca catacgcacg gtttttcgac aggtgatatg cgctgaaccc
     2821 gcatgaatac tggatttcca cagggttgag cggggttttg ctaaagcttg cgaacctgct
     2881 tttttgtaac ctgttgtttt tcatgatttt ttgttttgaa caccccaagt tttccacagg
     2941 ctcgccgtta agtttttaga tcttgttttt aagatcttgt ttttaatact tgttttaggg
     3001 ccgaattttc ttatgaaatt caagagttta gaagactata ggtaatataa tttaaggtaa
     3061 aggcaatatg atttaaggtg gataggcaat atgatttaag gtaaaggcaa tatgatttaa
     3121 ggtaatattc acaggaaaag ggatattaaa attaaatcat attgccttta cctaaaggaa
     3181 tgtttatatt ttgctcatta ccttaggttg agggatttac aatgtcggat ctgattgcct
     3241 acaaatccaa tgcactggtt gaggccagct ataaattgac tcttcaggaa cagcgattct
     3301 tgttactttg cattggtcga ttgaagtcgg gttctgatgc agaatccact gaattgcaaa
     3361 agaccatgac tgttaccgcc gaagagtatt tcgactcatt ccctgatatg gggcggaaaa
     3421 atgccgaggt tcagctacag gaagcaattg atcgtttgtg ggacaggtcg atcatcctta
     3481 aggacgatga gaagcgcgag gagttccgct ggatccagta tcgggcacag tacgctaaag
     3541 gtgagggcaa agcccaaatc actttttcag atgcggtcat gccttatctg acacagttga
     3601 aggggcaatt tactagagtt gtaattaaga acatctccaa tttatcaaga agttacagca
     3661 taagaatata cgaacttctc cagcaattcc gcagcacagg ggaacgtatt atcgccctcg
     3721 atgattttcg ctcaatgctt gatatagagc ataaatatca aacatataag tctctgaacc
     3781 agcaattatt aagaccttgc gttgacgaac tgaatcaaaa aagcgacttg gctgtaacgc
     3841 ttgagacgat caagaagggg cgcacggttg tagccctgca ttttcgcttc aaggaagaca
     3901 aacagatcaa gatgacgatc tagaatcgat tttaaggcct ctaaaacgct ctctgacgcg
     3961 ttttttttgt gtttggcatg cgaaggtact ggtgaagtaa aaaattgcgt cataaacgct
     4021 ctggcacagt gaaaaaagaa cccccaattt ttcgggggcg ggaaaactat aaagttgatt
     4081 tgtgttatgt g
//
