LOCUS       NZ_CP034386             6673 bp    DNA     circular CON 25-JUN-2024
DEFINITION  Escherichia coli O157:H7 strain FRIK804 plasmid pFRIK804-01,
            complete sequence.
ACCESSION   NZ_CP034386
VERSION     NZ_CP034386.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN10531264
            Assembly: GCF_009931235.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli O157:H7
  ORGANISM  Escherichia coli O157:H7
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6673)
  AUTHORS   Stanton,E., Wahlig,T., Park,D.-J. and Kaspar,C.W.
  TITLE     Escherichia coli O157:H7 strain FRIK804
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6673)
  AUTHORS   Stanton,E., Wahlig,T., Park,D.-J. and Kaspar,C.W.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-DEC-2018) Bacteriology, University of
            Wisconsin-Madison, 1550 Linden Dr., Madison, WI 53706, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 16-MAY-2018
            Assembly Method                   :: Canu v. 1.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 36.51x
            Sequencing Technology             :: PacBio RSII; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009931235.1-RS_2024_06_25
            Annotation Date                   :: 06/25/2024 01:58:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,666
            CDSs (total)                      :: 5,536
            Genes (coding)                    :: 5,219
            CDSs (with protein)               :: 5,219
            Genes (RNA)                       :: 130
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 101
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 317
            CDSs (without protein)            :: 317
            Pseudo Genes (ambiguous residues) :: 0 of 317
            Pseudo Genes (frameshifted)       :: 126 of 317
            Pseudo Genes (incomplete)         :: 175 of 317
            Pseudo Genes (internal stop)      :: 70 of 317
            Pseudo Genes (multiple problems)  :: 44 of 317
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP034386.1.
            Bacteria and source DNA available from Charles W. Kaspar.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6673
                     /organism="Escherichia coli O157:H7"
                     /mol_type="genomic DNA"
                     /strain="FRIK804"
                     /isolation_source="Calf"
                     /db_xref="taxon:83334"
                     /plasmid="pFRIK804-01"
                     /geo_loc_name="USA:Wisconsin"
                     /lat_lon="43 N 89 W"
                     /collection_date="1994-10-27"
                     /collected_by="Charles W. Kaspar"
     gene            complement(4..195)
                     /locus_tag="EJB16_RS27915"
                     /old_locus_tag="EJB16_28870"
     CDS             complement(4..195)
                     /locus_tag="EJB16_RS27915"
                     /old_locus_tag="EJB16_28870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165972.1"
                     /translation="MTKQEKTALNMARFIKSQTLTLLEKLNELDADDQADICEALHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            263..586
                     /locus_tag="EJB16_RS27920"
                     /old_locus_tag="EJB16_28875"
     CDS             263..586
                     /locus_tag="EJB16_RS27920"
                     /old_locus_tag="EJB16_28875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001444404.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPTL
                     APPLLRQFASVGNNLNQIARKINSGQWSGHDRVHVVAALMAIGRELSELRDEVRKQGER
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            795..>1229
                     /locus_tag="EJB16_RS28695"
                     /pseudo=""
     CDS             795..>1229
                     /locus_tag="EJB16_RS28695"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1282..1824
                     /locus_tag="EJB16_RS27930"
     CDS             1282..1824
                     /locus_tag="EJB16_RS27930"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_100029810.1"
                     /translation="MGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLNGQKIRDA
                     ISAHTAAVKEQLETLNSAVNAQFSATENELARRQEELLWKLVKGRILYPSLTALCVTVG
                     IFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKFLVLPSGVKG
                     ENNWTVEKKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            1831..2064
                     /locus_tag="EJB16_RS27935"
     CDS             1831..2064
                     /locus_tag="EJB16_RS27935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_001345152.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQENG
                     QLREQCDGLSQQVQRLAGQVDRLSRLFITNSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2554..2799)
                     /locus_tag="EJB16_RS27940"
                     /old_locus_tag="EJB16_28890"
     CDS             complement(2554..2799)
                     /locus_tag="EJB16_RS27940"
                     /old_locus_tag="EJB16_28890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000820629.1"
                     /translation="MKRHRLTEAEERRLAEAIAAEARAMGLTKKDKMPQETKRRLRARI
                     LKAILDERTASKNREATLTAGQDPDDTFTWQPLRRR"
                     /name="hypothetical protein"
     gene            3063..5156
                     /gene="cda"
                     /locus_tag="EJB16_RS27945"
                     /old_locus_tag="EJB16_28895"
     CDS             3063..5156
                     /gene="cda"
                     /locus_tag="EJB16_RS27945"
                     /old_locus_tag="EJB16_28895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-D"
                     /protein_id="WP_001286660.1"
                     /translation="MSDYEGSGPTEGIDYGHSMVVWPSTGLISGGDVKPGGSSGIAPSM
                     PPGWGDYSPQGIALVQSVLFPGIIRRIILDKELEEGDWSGWSVSVHSPWGNEKVSAART
                     VLENGLRGGLPEPSRPAAVSFARLEPASGNEQKIIRLMVTQQLEQVTDIPASQLPAAGN
                     NVPVKYRLMDLMQNGTQYMAIIGGIPMTVPVVDAVPVPDRSRPGTNIKDVYSAPVSPNL
                     PDLVLSVGQMNTPVLSNPEIQEEGVIAETGNYVEAGYTMSSNNHDVIVRFPEGSGVSPL
                     YISTVEILDSNGLSQRQEAENKAKDDFRVKKEEAVARAEAEKAKAELFSKAGVNQPPVY
                     TQEMMERANSVMNEQGALVLNNTASSVQLAMTGTGVWTAAGDIAGNISKFFSNALEKVT
                     IPEVSPLLMRISLGALWFHSEEAGAGSDIVPGRNLEAMFSLSAQMLAGQGVVIEPGATS
                     VNLPVRGQLINSNGQLALDLLKTGNESIPAAVPVLNAVRDTATGLDKITLPAVVGAPSR
                     TILVNPVPQPSVPTDTGNHQPVPVTPVHTGTEVKPVEMPVTTITPVSDVGGLRDFIYWR
                     PDAAGTGVEAVYVMLNEPLDSGRFSRKQLDKKYKHAGDFGISDTKKNRETLTKFRDAIE
                     EHLSDKDTVEKGTYRREKGSKVYFNPNTMNVVIIKSNGEFLSGWKINPDADNGRIYLET
                     GEL"
                     /name="cda"
     gene            5153..5416
                     /locus_tag="EJB16_RS27950"
                     /old_locus_tag="EJB16_28900"
     CDS             5153..5416
                     /locus_tag="EJB16_RS27950"
                     /old_locus_tag="EJB16_28900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032488334.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin immunity domain-containing protein"
                     /protein_id="WP_001038407.1"
                     /translation="MNKMAMIDLAKLFLASKITAIEFSERICVERRRLYGVKDLSPNIL
                     NCGEELFMAAERFEPDADRADYEIDDNGLKVEVRSILEKFKL"
                     /name="colicin immunity domain-containing protein"
     gene            5486..5632
                     /locus_tag="EJB16_RS27955"
                     /old_locus_tag="EJB16_28905"
     CDS             5486..5632
                     /locus_tag="EJB16_RS27955"
                     /old_locus_tag="EJB16_28905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711480.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_000726755.1"
                     /translation="MKKKIGGSMILVLAVLCLTACQANYVRDVQGGTIAPSSSSKLIGV
                     AVQ"
                     /name="colicin release lysis protein"
     BGC             1..6673
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          6508..6592
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="3.03e-37"
     Typing          5862..6046
                     /name="rep_cluster_2401"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.774"
                     /coverage="98.0"
                     /evalue="2.18e-82"
     Typing          577..2171
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.872"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gtgtcagagg ttttcaccgt catccccgaa gcgtgcgagg cagctgcggt aaagctcgtc
       61 agcgtggtcg tgaagcgctt cgcagatgtc agcctggtcg tcggcgtcca gttcattcag
      121 tttttccagc agggtcagcg tctggctttt gatgaagcgg gccatgttaa gggcggtttt
      181 ttcctgttta gtcacagttc tgtttctcca tgtgtgggga gtctgtcgga ggtttttaat
      241 gataccggtg aaacgcgaaa ggatgctcac gatacgggtc acggatgagg aacatgcgcg
      301 gctgctggca cgttgtgagg gaaaacaact tgcgtcatgg atgcgtaagg tctgcctggg
      361 ggcaccgcca tcgaaaacgt caggtcttcc gacactggca ccgccgctgt tgcgccagtt
      421 cgcttcggtc ggcaataacc tgaatcagat agcccggaag ataaacagcg gacagtggtc
      481 ggggcatgac cgggttcatg tggtggctgc actgatggcc atcggacgtg aactgagtga
      541 gctgagggat gaggtccgta aacagggaga acgggatgat agttaagttt catgccaggg
      601 gaaaaggcgg tggcagtggt ccggttgatt acctgctggg ccgggagcgt agccgggaag
      661 gggcgagagt tctgcggggt gctcccgaag aggtgcggga actcatcgat gccacgccgt
      721 ttgcgaaaaa atacacctcg ggggttctgt cttttgcaga gcagacactg ccaccgggag
      781 agcgtgaaag ggtgatggag agctttgagc gggtactgat gcccggtctt gaaaaaaatc
      841 agtacagcat cctgtgggtg gagcaccagg acaaggggcg tcttgagctg aattttgtca
      901 tcccgaatat ggagctggcg agcggaaaac ggctccagcc ctattacgac cgggcggacc
      961 gtccgcgaat caatgcctgg cagacactgg tgaatcacca ttacgggctg cacgacccga
     1021 acgcaccgga gaacaggcgg acacttgtca ccccgaataa tctgccgaaa gcgaaacagg
     1081 aggccgcaga agcgattacg aggggagtgg aggctcttta ccatgccgga gcgctaaaaa
     1141 cgcgtcagga tgtcactgag gcgcttacag cggcaggttt tgaggtggtg aggacaacca
     1201 ggagcagtat cagtatcgcc gacccggagg gcgggcgtaa tctcaggctg aaaggggcgc
     1261 tgtatgagca atcttttaca aatggggaca gacttcgaga agaaactgaa agagcgtgcc
     1321 gtctctacag agaacatgct gaacagcgag ttcaggaagc tcgaagcgtc tgtagacagg
     1381 gaactgaact taaacgggca gaaaatcaga gacgctatca gcgcacacac agcagcggtg
     1441 aaagaacaac tggagacgct gaacagtgcc gtgaacgcgc agttcagcgc cacagagaac
     1501 gaacttgccc ggcggcagga agagctgctc tggaagctgg tcaaagggcg gatactgtac
     1561 ccgtcgctga cggcgctttg cgtcacggtc gggatttttc tggcgtcatg ggggttgatt
     1621 cagtggcagg aaagccgtat tgcggcgaac atcctggcta tcagggacca ggaagagacg
     1681 ctggcgaaac tccgggaaaa aacatggggt gtgacgtatc acgagggcag gaacgggaaa
     1741 tttctggtgt tgccgtccgg ggtgaagggg gaaaacaact ggacggtgga gaagaagaac
     1801 gcagtaagac tggtcaggga gtaagtgagt atgacagagc tggaaatgca cttactgaac
     1861 gccttagagc aattgcagca ggactatacg cagcggctga aagactggga gagcgccttc
     1921 acggatttgc agcatatgtt cgagctgaca cgacaggaga acgggcaact gagggaacag
     1981 tgcgacggct tgagtcagca ggtgcagaga ttagccgggc aggtggaccg cttgagtcgg
     2041 ttatttatca cgaacagcag ataagggatg aacaggaaag gctggcacgc ctggaacggg
     2101 agcgtgcgcg gtcctggcat gaagagcagg aacgggaagt acgggtatgg cgtggcccgt
     2161 caatggggat gtgacggtga gttgttccgg gaggttgcca gatggtaccg gaacgcgtta
     2221 aaaacgaatc cgggcgcgat tctggcgtgt cgtttttgtt gctgtgtttt gcctctttgc
     2281 cagatcgcgc tcattcctca gcctcatccc gcgatgtctg cgtatgtcat caactgaaaa
     2341 gatggaaaaa aaacagtgtc atgaggttta cgcaggcaaa accagcgtta ttcacatggc
     2401 tgaatagcta tgcatacggt taaaatttat caggtgcgat cgcggcagct tttcgggtgg
     2461 tttgttgcca tttttacctg tcagcagccg tgatcgcgct gaacgcattt cagcggtgcg
     2521 tacaataagg ggttatgtta aataaccctt agcttagcgg cggcgcagtg gttgccaggt
     2581 gaaggtgtca tccgggtcct gtccggcagt cagtgtggct tctcgatttt tgctggccgt
     2641 tcgttcatca agaatggctt taagtatccg tgcccggagg cgtcgctttg tttcctgcgg
     2701 cattttgtcc tttttcgtca gccccatggc acgagcttca gcggcaatgg cttctgccag
     2761 tcggcgctct tcggcttcgg tcagtctatg gcgtttcatg gtggtctccg ggtgtttctc
     2821 tggtgtcata acagtttaac tgttggctct ctccttatgc taatcagcct tctttcatgc
     2881 ctgttgccat tgagtttaag aatgattggt tgtttttctt gagcgttttc acaaaaacgt
     2941 aaatccagtg ggttgacagg atttacgagg tgacgtaact ttttactgta cataaaacca
     3001 gtggttttat gtacagtttg ttgtttttaa cgcattgttt taaaagtcaa agaggtgttt
     3061 ttatgagtga ttacgaaggt agtggtccga cagaaggtat tgattatggg cactcgatgg
     3121 tcgtgtggcc gtcaacagga ctgatttcgg gtggtgatgt gaaaccagga ggctcatcag
     3181 gtatcgctcc atccatgcct ccgggatggg gggattacag cccacagggt atcgcacttg
     3241 tacaaagtgt tctttttccg ggaattattc gccggattat tctggataag gaacttgaag
     3301 agggagactg gtcgggatgg tctgtcagtg tgcatagccc ttggggaaac gagaaagttt
     3361 ccgctgcacg aacagttctt gagaatggtt tacgtggtgg tttgccagaa ccgtctcgcc
     3421 cggctgctgt ttcttttgcc cgtctggagc ctgcttccgg aaatgagcaa aaaattattc
     3481 gtcttatggt tacacagcaa ctggaacagg taacggatat tccggccagc cagttaccag
     3541 cagcgggtaa taatgtaccg gtgaaatatc gtctgatgga ccttatgcag aacggtacgc
     3601 aatatatggc tattatcgga ggtattccga tgacagtgcc ggtagttgat gccgttccag
     3661 ttccggaccg gagtcgtccg ggaacaaata ttaaagatgt ttacagtgcc cctgtatcac
     3721 caaatctacc ggacctggta ttaagtgtgg gtcagatgaa tactccggtt ctgtctaatc
     3781 ccgaaattca ggaagaagga gttattgctg agacaggtaa ttatgttgag gctggttata
     3841 cgatgtccag taataatcat gatgtcattg ttcgttttcc tgaaggcagt ggtgtttctc
     3901 cgttatatat ttcaactgta gagattctgg acagtaatgg tctgagtcag cgtcaggaag
     3961 ccgaaaataa agcaaaggat gatttcagag tcaagaaaga agaagcggta gcccgggctg
     4021 aagctgaaaa agcaaaagcc gagttattta gtaaagcagg tgtgaaccag cctcctgtat
     4081 atacacagga aatgatggaa agggctaatt cagtaatgaa tgagcaggga gctcttgttc
     4141 tgaataacac tgccagttct gtacaactgg cgatgaccgg aactggtgtc tggactgctg
     4201 ctggtgatat tgcaggcaac ataagcaagt ttttcagtaa tgctctggag aaagtaacca
     4261 tacccgaagt gagtcccctg cttatgcgga tttctcttgg tgctctgtgg tttcattcag
     4321 aagaagctgg agcaggaagt gatatcgtgc cggggcgaaa tctggaggcg atgttttcgc
     4381 tgagtgctca gatgttggct ggacagggcg tggtcattga acctggtgcg acgagtgtaa
     4441 atctgcctgt tcgtggacaa ttgataaaca gtaacgggca attagctctg gatttactga
     4501 aaacagggaa tgaaagtatc ccagctgcgg ttcctgttct taatgctgtc cgtgatacag
     4561 caacaggact ggataaaatc acgttaccag cagtagtagg cgcaccttcc cggacgattc
     4621 tggttaatcc ggtaccacaa ccttcggtgc caacagatac aggtaatcat caacccgttc
     4681 cggttacacc agtgcataca ggaacggaag taaaaccggt cgaaatgcca gtaacgacga
     4741 ttactcctgt ttctgatgtt ggtggactac gagattttat ttactggcgt cctgatgctg
     4801 ctgggactgg tgttgaagct gtttatgtga tgcttaatga acctctggat tcagggcgat
     4861 tttcgcgtaa acaacttgat aaaaaatata aacatgctgg tgattttggt attagtgata
     4921 caaaaaagaa tcgtgaaact cttactaaat tcagggatgc tattgaggag catttatcgg
     4981 ataaggatac agtagagaaa ggaacatacc gaagagaaaa aggttcaaaa gtttatttta
     5041 atcctaatac gatgaatgtg gttataatta agtcaaatgg tgagttctta tctgggtgga
     5101 aaataaatcc agatgcggat aatggtcgaa tttatttaga gacaggtgaa ctatgaacaa
     5161 gatggcaatg atcgatttgg cgaaattatt tttagcttcg aaaattacag caattgagtt
     5221 ttcagagcga atttgtgttg aacggagaag attgtatggt gttaaggatt tgtctccgaa
     5281 tatattaaat tgtggggaag agttgtttat ggctgctgag cgatttgagc ctgatgcaga
     5341 tagggctgat tatgaaattg atgataatgg acttaaggtt gaggtccgat ctatcttgga
     5401 aaaatttaaa ttataatcat gagtgatttt aatatctggc atttaagtat aaatgtcaga
     5461 tattcactca tgacaaggag atgtcatgaa aaaaaagata gggggatcta tgattctcgt
     5521 tcttgctgtt ttgtgtctga ctgcttgcca agctaactat gtaagagatg ttcagggtgg
     5581 aacaattgct ccatcatcat cttcgaaact aatcggggtg gcggttcagt gaaaaatatt
     5641 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcctgta aacgaaaaaa
     5701 cccgccttgt ggcgggtcct tccgtccgat cagaagtctt cgacctcttc taatcggcgg
     5761 tggtaactgg cttggaggag cacagtcacc aaaactgtct tttcagttta gccttaactg
     5821 gttcataact tcaagagcaa ctcctctaaa tcagttacca atggctgctg ccagtggcgt
     5881 taagtcgtgt cttttccggg ttggccccaa gagaacagtt accggagaag gtgcagcagt
     5941 cgggctgaac ggggggttcc tgcatacagc ccagcccgga gcgaactgcc taagtcgccc
     6001 cgagatacca gaagacgtga gctatgagaa agcgccacgt gtcccgcaag ggagaaaggc
     6061 ggaaaaggta tccggtaatc gatatggcgg gaacgtgggg gcgcaggagg gagcgattca
     6121 ccggaaacgg tgatgatctt taagccacgt cgggtttcgc caccgctggc ggatttcatg
     6181 gttgagcctc atggctccca cagatgcaca gaaaaagcgt ctgttttgtg aagtccgtca
     6241 ggggggcgga gcctatggaa aaacgccagc ggcgcgactt tttttgccct ttgccttttt
     6301 gctcacatgt tctttccggc cttatcccct gattctgtgg ataacactat caccgctttt
     6361 gagtgggcag ataccgctcg ccgcagccga acgaccgagc gtagcgagtc agtgagcgag
     6421 gaagcggaaa agcgcctgga cgagcatttt ctccttacgc atctgtgcgg catttcacac
     6481 ccgccatggc gcacttttca tacaattctc actgatgcca tatgattaag ccagtataca
     6541 ctccgctatc gctatgtgac tgggtcatgg ctgcgccccg acacccgcta aaacccgctg
     6601 acgcgccctg acgggcttgt ctgctcccgg catccgctca caggccagct gtgaccgtct
     6661 ccgggagctg cat
//
