LOCUS       NZ_CP034135             3733 bp    DNA     circular CON 26-NOV-2023
DEFINITION  Klebsiella quasipneumoniae strain G4584 plasmid pG4584_3.7Kb,
            complete sequence.
ACCESSION   NZ_CP034135
VERSION     NZ_CP034135.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN05960932
            Assembly: GCF_002239895.2
KEYWORDS    RefSeq.
SOURCE      Klebsiella quasipneumoniae
  ORGANISM  Klebsiella quasipneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3733)
  AUTHORS   White,R., Fouts,D. and nguyen,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-NOV-2018) Informatics Core Services, JCVI, 9605
            Medical Center Drive, Rockville, MD 20850, USA
REFERENCE   2  (bases 1 to 3733)
  AUTHORS   Fouts,D., Brinkac,L., Sutton,G., Nguyen,K., Venepally,P., Wright,M.,
            Clarissa,I., Olanipekun,G., Medugu,N., Kocmich,N.,
            Rezac-Elgohary,A., Murphy,C., Fey,P. and Obaro,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JUN-2017) Informatics Core Services, JCVI, 9704
            Medical Center Drive, Rockville, MD 20850, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.6
            Assembly Name                     :: G4584
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 759.556x
            Sequencing Technology :: Illumina NextSeq and Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/26/2023 02:40:44
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,990
            CDSs (total)                      :: 5,872
            Genes (coding)                    :: 5,658
            CDSs (with protein)               :: 5,658
            Genes (RNA)                       :: 118
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 214
            CDSs (without protein)            :: 214
            Pseudo Genes (ambiguous residues) :: 0 of 214
            Pseudo Genes (frameshifted)       :: 79 of 214
            Pseudo Genes (incomplete)         :: 144 of 214
            Pseudo Genes (internal stop)      :: 32 of 214
            Pseudo Genes (multiple problems)  :: 39 of 214
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP034135.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3733
                     /organism="Klebsiella quasipneumoniae"
                     /mol_type="genomic DNA"
                     /strain="G4584"
                     /isolation_source="Blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1463165"
                     /plasmid="pG4584_3.7Kb"
                     /geo_loc_name="Nigeria"
                     /collection_date="2016-04-15"
                     /collected_by="Stephen Obaro"
     gene            1..114
                     /locus_tag="BME54_RS32025"
                     /old_locus_tag="BME54_30660"
     CDS             1..114
                     /locus_tag="BME54_RS32025"
                     /old_locus_tag="BME54_30660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_087812787.1"
                     /translation="MHEPPVQSDRCAFSGNYRLESNPVRHAKAPLAAATGN"
                     /name="replication initiation protein"
     gene            complement(269..736)
                     /locus_tag="BME54_RS30665"
                     /old_locus_tag="BME54_30665"
     CDS             complement(269..736)
                     /locus_tag="BME54_RS30665"
                     /old_locus_tag="BME54_30665"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="WP_087812786.1"
                     /translation="MRKASLGSEGIGGLIENLPQMLHGFSEQLHSVSILMSACMEPVTD
                     DHDLQERMAIIDELYSCAEDIDHVAIKFADLVTDRVYEYEKKNRNVPNVSPSEALSYFI
                     KERGLRQADLKSVASQSIISELINGKRVMNIDQVKGFAAFFDVPVETFMGT"
                     /name="transcriptional regulator"
     gene            complement(740..1111)
                     /locus_tag="BME54_RS30670"
                     /old_locus_tag="BME54_30670"
     CDS             complement(740..1111)
                     /locus_tag="BME54_RS30670"
                     /old_locus_tag="BME54_30670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008086990.1"
                     /GO_component="GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /GO_function="GO:0004519 - endonuclease activity [Evidence
                     IEA]; GO:0016788 - hydrolase activity, acting on ester
                     bonds [Evidence IEA]; GO:0003723 - RNA binding [Evidence
                     IEA]"
                     /GO_process="GO:0090305 - nucleic acid phosphodiester bond
                     hydrolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family toxin"
                     /protein_id="WP_087812785.1"
                     /translation="MRVVTARTITDAMTVHAQWKVGLKLWLDVFDKQSLIFESFSQLRD
                     VWLSKSGWNVDRIPCSLLKAESKKGPLDIYVFDIHKNQCRVVVWLNPKSGTFFVKDVCS
                     HAEYDRWWRGQTKSQRSKG"
                     /name="type II toxin-antitoxin system HigB family toxin"
     gene            complement(1172..1741)
                     /locus_tag="BME54_RS30675"
                     /old_locus_tag="BME54_30675"
     CDS             complement(1172..1741)
                     /locus_tag="BME54_RS30675"
                     /old_locus_tag="BME54_30675"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_124982953.1"
                     /translation="MSSDDSKISTDAIGDVNEYLLSPTKGGALKVTIPDDLNDDLSAYA
                     AGYNTSKSEVVRNSVASFMGNDLRWTHRQYFFRPRRKLVSFEDLRMCTGFVQVACFPKD
                     LPRNASGVTVKLLKYEKNFSIITFDLGNGVVPHPPYPIQGGDIDLGPGSLVMPTVEQST
                     NIYTMNIIYYRINNDLIWGVCPAISN"
                     /name="hypothetical protein"
     gene            complement(2705..3127)
                     /locus_tag="BME54_RS30685"
                     /old_locus_tag="BME54_30685"
     CDS             complement(2705..3127)
                     /locus_tag="BME54_RS30685"
                     /old_locus_tag="BME54_30685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_069346495.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKAIKVID
                     TSELIRVFGSLDGVIDTVKYDVKSDAESTSINSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKHEPSSPPHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(3127..3318)
                     /locus_tag="BME54_RS30690"
                     /old_locus_tag="BME54_30690"
     CDS             complement(3127..3318)
                     /locus_tag="BME54_RS30690"
                     /old_locus_tag="BME54_30690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_087812789.1"
                     /translation="MRKEQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          2..114
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.737"
                     /coverage="100.0"
                     /evalue="1.2399999999999998e-42"
     Typing          3440..3733
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.22"
                     /coverage="99.0"
                     /evalue="2.96e-119"
ORIGIN
        1 atgcacgaac cccccgttca gtccgaccgc tgcgccttct ccggtaacta tcgtcttgag
       61 tccaacccgg taagacatgc aaaagcgcca ctggcagcag ccactggtaa ctgatttaga
      121 ggagttagtc ttgaagttat gcatcaatta aagctaaact gaaaggacca atttggtgac
      181 taagctcctc caagctcgtt accgcaggtt ttatgagttg ctagctcaac gaacctaaaa
      241 aaaccaccgc taccaacggt ggtttttttt atgtccccat aaacgtttcc acaggaacat
      301 caaaaaacgc agcaaatcct ttcacctgat ctatgttcat aacccttttt ccattgatga
      361 gttcagatat gatgctctga ctagcaacag atttaaggtc agcttgtcga agcccccgtt
      421 cctttatgaa ataagacaac gcctcactgg ggctgacgtt aggaacgtta cgatttttct
      481 tctcgtactc atagactcga tcggttacaa gatcggcaaa ttttatagcg acatgatcga
      541 tatcttctgc acaagaatac aattcatcaa taatcgccat acgttcttgg aggtcatgat
      601 catcggtgac tggctccata catgcagaca ttaagataga cactgaatgc aactgttcag
      661 aaaaaccatg taacatctgc ggtaaatttt cgatgagccc accaattcct tcagaaccga
      721 gtgatgcttt tctcataatt cacccttttg acctttgaga tttagtttgc cccctccacc
      781 atcgatcata ttcggcatga gaacaaacat ctttgacaaa aaaagtacca gacttagggt
      841 tcaaccagac aacaactctg cattgattct tgtgtatatc aaaaacatat atatcaagag
      901 gccccttctt actttctgcc tttaacaggc tacaaggtat gcgatcgaca ttccatcctg
      961 atttactaag ccaaacatcc cgaagttgag aaaatgactc aaaaatcaaa gattgcttat
     1021 caaagacatc aagccataat ttgagtccaa ctttccactg ggcatgaaca gtcatagcat
     1081 cagtgatagt tctagccgta acaacgcgca taacctaccc ctataatatc gcaatttgcg
     1141 atatttgcaa aataaagcga tttaaattta ttcaattact aatcgctgga cacacacccc
     1201 agattaagtc attgtttatt ctgtaatata taatgttcat tgtatagata ttggtactct
     1261 gttctacggt gggcataact aaagaaccag gtccgagatc gatatccccc ccctgaatcg
     1321 gatagggtgg gtgaggtaca acaccatttc caagatcaaa agttataatc gaaaaatttt
     1381 tctcatattt aagtaactta actgtaactc cagaggcatt ccttggaaga tcttttggaa
     1441 aacaagcgac ttgaacaaag ccagtacaca ttctaagatc ttcaaaacta actaatttcc
     1501 tacgtggtcg gaaaaagtat tgccgatgag tccagcgtag atcgttcccc atgaaactag
     1561 ctacgctatt cctcactact tcagactttg atgtgttgta accagctgcg taggctgata
     1621 agtcatcatt tagatcatca gggatggtta ccttcaacgc ccccccttta gtcggagata
     1681 aaagatattc atttacatcc ccgatggcat cggtagaaat ttttgaatca tcgctagaca
     1741 tataccaccc cgcaaaaaga acaagaacgg acacatagtg gaacttttta gtccacatgt
     1801 aaacacttta aagatcataa aagaataatt caactaatca catccatata aaactgaact
     1861 gaataaaatg gtcacgaaag ggtctgagga aaggtcgcca agaagccagc cgcttagtcc
     1921 ctcagcagaa agtcccttta gccccttcaa tggcatttta cgattattat ttaacataaa
     1981 gccctgattg tacgcaccgc taaaacggcc tcagggcgtt tctgacggga aaccggtaaa
     2041 acaggcaaca aaccacccga aaacccgcca gaaacgcgca ggctctgttt ttacggcatg
     2101 catgagtatg cgcgcaagtg catagcgcgg aaattcacgc cattcagcca gggtaagtcc
     2161 tgtttccggc gttcttctga tccgaaaacg gtgaacgggg acgcaattta ttgcgtggcc
     2221 gtgaggcgtt gccggcgcag ccggaaaacc ggttttcagc acgttcagga atcgggttcg
     2281 tcgacggccg gcaatcacgt tatggcaagt cgccggacga ctcaccacac gattcaccgg
     2341 cgattcgaat cgtgaattga atcaagagtc accatgaaca aaaacgccca aattgatgaa
     2401 ttgaatcaag agtcgattca tcataacaac atgataataa agcgtgattc acatatgaat
     2461 cgtgggcaat tcgtaaacga ttcgccatag agtcgtccac gacttggatc tgcactttgt
     2521 aacacatcac tgcacattaa tttttcatta acaaattgtt ttaaataagg aatctgcaaa
     2581 attttaattt tgcgtagtgt gtgggtattt ttagtggtga ggagcgttca gagatctggc
     2641 gattttttgg aaggttcagg aactccccgg aatggccgga gaggaaaact gaagcatgac
     2701 ggggtcagga ttttttccag aatttccacc acggggaatg gggtggtgaa gaaggttcgt
     2761 gcttgtgctc cagaagctgc atagcttgac gaagggaatc aatatgctca tctcgagcct
     2821 taacagcccc tttaagacca tcgttttcag cttcaagtag agcgattcta tgttgtaaat
     2881 catgtaaacc aacgctgtta atacttgtag attctgcgtc actttttaca tcatatttta
     2941 cagtatcgat gacaccatca agagaaccaa acactctaat aagctctgaa gtatcaataa
     3001 ctttaatagc cttcccgtgc tttacaacag aggtgtaact taacttccca ctgttaatca
     3061 tcttgtaaat tgtggggcga ctaaccttaa caagccgtgc cgcttcacta atagacactc
     3121 tggccatcag ttaccatcct gtaattcatc cagtctcgag gacagtgtac ggaaaagatg
     3181 ctccgcatca tcgtggagtt tctcacaaag gtcggcctcg acatcgagat caagctcgtt
     3241 aagcttctcc aacaagagca gagactgatt ctggatgaat ttagccatgt tcagcgctgt
     3301 tttctgttct ttcctcataa agggcctcag agcgtcagtg ctcaatgtca gacaggtctc
     3361 ttttccgctt cctcgctcac tcagtagcta cgctcctgcc gttcggctgc ggcgagcggc
     3421 aggagcatgc gtagaaacgc tcgagaaaac cgagcaatcg tatgatggag agaaggaaag
     3481 gccgcacggc ggccgttttt ccataggctc cgcccccctg acgagcatca cggaatctga
     3541 cgctcaaatc agtggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     3601 gatggctccc tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt cattccgctg
     3661 ttatggccgc gtttgtctca ttccacgcct gacactcagt tccgggtagg cagttcgctc
     3721 caagctggac tgt
//
