LOCUS       NZ_CP034127             5596 bp    DNA     circular CON 05-JAN-2024
DEFINITION  Klebsiella pneumoniae strain BJCFK909 plasmid p4s2, complete
            sequence.
ACCESSION   NZ_CP034127
VERSION     NZ_CP034127.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN10473953
            Assembly: GCF_003856475.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Dong,S.
  TITLE     Whole genome of Klebsiella pneumoniae strain BJCFK909
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Dong,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-NOV-2018) Department of Clinical Laboratory, Zhejiang
            Hospital, 12 Lingyin Road, Xihu District, Hangzhou, Zhejiang 310013,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: minimap v. 2.0; miniasm v. 2.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 300.0x
            Sequencing Technology :: Oxford Nanopore GridION; Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/04/2024 21:42:22
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,908
            CDSs (total)                      :: 5,786
            Genes (coding)                    :: 5,607
            CDSs (with protein)               :: 5,607
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 69 of 179
            Pseudo Genes (incomplete)         :: 117 of 179
            Pseudo Genes (internal stop)      :: 26 of 179
            Pseudo Genes (multiple problems)  :: 33 of 179
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP034127.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="BJCFK909"
                     /isolation_source="respiratory tract"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p4s2"
                     /geo_loc_name="China: zhejiang"
                     /lat_lon="30.17 N 120.09 E"
                     /collection_date="2018-09"
                     /collected_by="shilei dong"
     gene            join(5272..5596,1..26)
                     /locus_tag="EH202_RS30235"
                     /old_locus_tag="EH202_30235"
     CDS             join(5272..5596,1..26)
                     /locus_tag="EH202_RS30235"
                     /old_locus_tag="EH202_30235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(96..362)
                     /locus_tag="EH202_RS30240"
                     /old_locus_tag="EH202_30240"
     CDS             complement(96..362)
                     /locus_tag="EH202_RS30240"
                     /old_locus_tag="EH202_30240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(346..570)
                     /locus_tag="EH202_RS30245"
                     /old_locus_tag="EH202_30245"
     CDS             complement(346..570)
                     /locus_tag="EH202_RS30245"
                     /old_locus_tag="EH202_30245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            complement(1237..1449)
                     /locus_tag="EH202_RS30250"
                     /old_locus_tag="EH202_30250"
     CDS             complement(1237..1449)
                     /locus_tag="EH202_RS30250"
                     /old_locus_tag="EH202_30250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1456..1965)
                     /locus_tag="EH202_RS30255"
                     /old_locus_tag="EH202_30255"
     CDS             complement(1456..1965)
                     /locus_tag="EH202_RS30255"
                     /old_locus_tag="EH202_30255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<2000..2704)
                     /locus_tag="EH202_RS32320"
                     /old_locus_tag="EH202_30260"
                     /pseudo=""
     CDS             complement(<2000..2704)
                     /locus_tag="EH202_RS32320"
                     /old_locus_tag="EH202_30260"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2643..2987)
                     /locus_tag="EH202_RS30265"
                     /old_locus_tag="EH202_30265"
     CDS             complement(2643..2987)
                     /locus_tag="EH202_RS30265"
                     /old_locus_tag="EH202_30265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(2977..3153)
                     /locus_tag="EH202_RS31590"
     CDS             complement(2977..3153)
                     /locus_tag="EH202_RS31590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            3793..3951
                     /locus_tag="EH202_RS32325"
     CDS             3793..3951
                     /locus_tag="EH202_RS32325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            4309..4635
                     /locus_tag="EH202_RS30275"
                     /old_locus_tag="EH202_30275"
     CDS             4309..4635
                     /locus_tag="EH202_RS30275"
                     /old_locus_tag="EH202_30275"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            4661..5206
                     /locus_tag="EH202_RS30280"
                     /old_locus_tag="EH202_30280"
     CDS             4661..5206
                     /locus_tag="EH202_RS30280"
                     /old_locus_tag="EH202_30280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     Typing          complement(3240..3316)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="91.25"
                     /coverage="98.0"
                     /evalue="1.04e-21"
     Typing          3505..3902
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          complement(1122..2653)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.804"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ggttaactaa aaccgacaaa cagtagaaca cgtagttagt cgagtattta ccataatacc
       61 tgttgtcggc atcaaaaaag cccgcatagc gggcttcagt cgtaaacttc gcggcgatgt
      121 ccaatcgtgg cggcaaggat aacaagctcg tcatctcgga tctcacacag aacgcgataa
      181 tctcccaccc gatagcgcca gaactcgccc agctcacctt tgagcggctt ccctgactgg
      241 cgaggatcgg cagcaactgc aatgcgtaaa ctcataaaat ccacaatccg tcgtgcgttc
      301 tgtttgtcca tcttgcgtaa cgatttgagc gcccgatcgg aatagttaat cgtccagacc
      361 aagtgacttc ctcacgtctt cagacgaatg cactgcttca tcaccacggc gaacgcgtgc
      421 agcagtttct gctgaaagat aataatcctc caggtcttcc agatgcgcca gtattgcttc
      481 ccgcgcataa aacgtctttg ttctgccggt ttgcttcgcc agcgagtcca gacgggactc
      541 aatctcatca gataaccgga tagcaagcat ataaacctcc cgaacaccat ttgctataca
      601 tgtatatcaa cagtaccaca atcaacctaa aagaacaaaa aataatcttg ttagcccctc
      661 ttgataatcc gtaacgcggt caatatatct agcttgaggg ctttcctctt aactattgaa
      721 aggataacga ttatgggtct taagccgggt ccgaaacgaa ttgccgattc aacaggggaa
      781 cctgataaac gccagcgtga caataaaaaa acgccgggaa atactgacaa attgaaaccc
      841 agcaaatctt caaaaaaata agtcagtagt taaaagccct ccggggcttt tttgtatctg
      901 ccgttcacgc tgattaaaca taacacccgt tgcccgcacc gccccggacg ggttcgccgc
      961 gaacggggtg gcagtcaggt aaaaccggca acaaaccacc cgataaaccg ccccgttcgc
     1021 gacagataaa ttttacctgg ctgcatagcc atgcagccag gtgaatgaca tccggtttca
     1081 gcgtaaatgc ccgatgagaa ccctgttttc ctgcatttca gcccagcgac ggaccgtcat
     1141 agcttcgctg ccgggaacgc gcttccgtca gttccttttc gtgctgccgt tgctcgtgcg
     1201 cccgttctgc ggccacggcc atctcatgac gctgcactac cggctcaaac gctgcagttg
     1261 cccgctcaag cgttccacct gctgactcaa gcgcgtcacg cgctcgctca gcgccgcgtt
     1321 ctcccgttgc ataagccctg acattttccg ccattccccg aaggcgctct cccactcgtc
     1381 cagccttttc gagtagtcct gctgtagctg ctctaatgcg ctcagcaact gtttttccag
     1441 ctcggtcatc ggcacttact ccttcaccag tctcagcgcg ttctgcttcg accgcccgtt
     1501 cccgaccgtc cagctctgcg tcctgtctac ggccgtcccc ttcggcagca ccagaaaacg
     1561 tccgttgctg tcttccaggt acgtcacgcc ccaggtcttc gcgttcagct tcgacagcgc
     1621 tgcgttctgc cggtcgattt cggccagatt tgacgctatc aagctcccct gataccacaa
     1681 caccccgctc aggctggcga acagcagtcc tgacaccatc gtgatggtca gccatgtccg
     1741 cccgaccata cgcatcacgc tcaggcggtt cgactgcatg gccgcgctca taccctgctc
     1801 gtgggcgcgg atggcggcgc tgattctctt cccgctttcg ctcagttccg ctctgacaga
     1861 cttctcgtgc tcgctgaatg cggctttcag catttcgccg gtactctgct gctgcgcttt
     1921 cgatttctgc tctaagtcct tcgccagcgt taaaagactg ttcatagatg gctcccttaa
     1981 gtcggatgtt tcgccctccg tccgggtcgg caatgctgat gctgctcttt gtggtgcgca
     2041 cgacctcaaa acctgcgctt tccagcgcct cagtgacgtc ctgacgcgtt ttaagctccc
     2101 ctgacgaggc gagggccagt aagccccgcg taatagcctc tgcggcttcc tgcttcgttt
     2161 tgggtaacga agatggcgtc accagcgcgc gccggttctc cggtgcgttc gggtcgtgca
     2221 gccccagtcg cccgttcacc acggtctgcc acgcatcgat gcgcggacgg tctgcccggt
     2281 cgtagtacgg ctggagacgc ctgccggtca gcagttccgt gttcggtacc agaaaattca
     2341 gttccagccg ccccttgtcc cggtgttcaa cccacagcac gctgtactgg tctttatcga
     2401 gtccgggcat cagaacccgc tcgaagctcg ccatcagctt ttcacgctgg ccgggcggta
     2461 aatcctgttc agcaaaagac aggacgccgg aggtgtactt tttggcgtag ggcgaggcgt
     2521 cgataagctc ccggacctcc tccggctttc cctgcaggac gatggccccg tcgcgctggc
     2581 ggtctttccc cagcagataa tccaccggcc cggcaccgcc gcctcgccct cgcggatgaa
     2641 acttaacgat catcgtctgc ccccttttcc agcacggcat gccgcagccg ctcgagttcg
     2701 gcatcaatgg ccatcagcgc ggccaccacc tgaacccggt cagcgcctga ccactggccg
     2761 ccgttaattt tccgggcgat ctggttgagg ttgttcccca tcccggcaag ctggcgcagc
     2821 aaaaccggat cgatggacgg cagccgcaac gaccgcgccg ggcgcgtatc gagacaggtt
     2881 tcccgcatcc acaccgccag ttgccttcca ttgcagcgct ccagcagctg ctggtactcc
     2941 ccatcggtca cccggatacc taaaaaacgg gtacgcttaa ccgccatcct ctcctccgat
     3001 atccgcaaaa tgtgaccggg ctgaacgctc aagctctgcc atctgctccc ggcggtaatc
     3061 ttcctcctgt gcctgacgca tcagatttgc catcactgcc ggatgccagg atcttttttt
     3121 tacgacgagc ggcttcttct tcggcttctg cattatccct ccttcgcaaa cgccggacgg
     3181 cacggaaaag ttttccgggt cgctccggcg ttggacaagc gccagcgcgt cagtggtgtt
     3241 agcgggtttc ggggcgcagc cctgaaccag tcacagcgtt tacgctgtgt atactggctt
     3301 actatgttgg catgagagca cgattcatga aagtgcctca tgtggcaggt gtgatatgtg
     3361 gctcaggagc gtccgcaata ccatgcacgg acagatgaaa tgacgcctcc tcgctcactc
     3421 ggtagctgcg ctcctgccgt tcggctgcgg cgggcgtgac cgactcacgg ataacgggat
     3481 aaagcaaagg cgctcaaatc agaaagggcc ggagaaaagg atgcggcgtt gccgtttttc
     3541 cataggctcc gcctcccctg acgagcatca cagaaatgga cgctcaagtc agaggaggac
     3601 gaaacccgac aggacttaaa gatgccggtg atttctctcc ggcggctccc tcgtgcgctc
     3661 tcctgttccg accctgccgt ttaccggata cctgttccgc ctttttccct ttcgggaagc
     3721 gtggcgcttt ctcatagctc acgctgctgg tatctcggct cggtttaggt cgttcgctcc
     3781 aagctgggct gtatgcacga accccccgtt cagcccgact gctgcgccct ttccggtaac
     3841 tatcatcttg agtccaaccc ggacggacac gcaaaaacgc cactggcagc agccattggt
     3901 aactggatat atacgagaga gatattgaga ggtcttgaag tgggtgccta actgcggcta
     3961 cactgaaaga acagttttgg tatctgcggt ctcgtatacc cagttacccg gttaagcagt
     4021 tccccaactg acttaacctt cgatcaaacc gcctccccag gcggtttttt cgtttacagt
     4081 gcaagagatt acgcgcagaa aaaaaggatc tcaagaagat cctttaaatg atcgtcagtt
     4141 ctgttatccc cggagctcca tccatcccac ggaaaaacac ccgccactga tgccctcagc
     4201 gcactgcaca tcatgtacaa accactccct ccctcctttt ttattgcgtt tcttaatgcg
     4261 caatggtagc tttgtgcgca acaaaaaacg caatagcaag ggggggtaat gagagaaaga
     4321 acaaatatca tgctagatat aggattaaag caatccttac aacggctttc gaataaaacc
     4381 aaaatgagtc taagtgatct tattaaccag agattatcag gttcactttc agttgaagaa
     4441 cgcattggcg aaaagcctga atggatgaga aaccttgatc cctatctcgc caatcacttt
     4501 tcaaatattg agttaccgct cccttcacca ttaaaagaat accaagaaaa aatggatgag
     4561 atagattcaa ttattaatga catgatggtt ctaaaggaat atttcagagc taagttttac
     4621 gacgaaataa aataggcacg ctatacggag gcggagaatt atgtttttta aacggtattt
     4681 aacggagaag gaaatataca aggcagcact cccaattgtt aggcttcaag ggagagttaa
     4741 cacttcatta cgttttttat ccaaatactc agatgtaaac aaaaatggat taattgcatt
     4801 gcatcttaat ttattacatt ttatctcata caacatacac ggtgtagagt ctctgaaaat
     4861 cactcaagca ggcataaaca aagctgtaca acaaagccct cacgcagatg aaataaaaaa
     4921 tattgcgcag gcaataggac gcaaaggacc tgagtacatc gaaggattaa ttagaaaccc
     4981 atgggggaag aaaaatgata atccactaat atttataatg agcattgaag aaagtataaa
     5041 agaaaccctt aactcaggtt atatgttata tagagtagct cccataactg agggtgaagc
     5101 agctgctttc atggatgttc ttaaacaatt attattattt gaggataatt ttcgcctttt
     5161 tggtagagac ttggtttata aaatagaaaa attaaaaatc agataacaaa gtatggtgaa
     5221 cgaaaaatga aagagaggat agtgaaatgt gtaataccaa gattctcaac tatgtattgg
     5281 ttaattgcgt tcctagcatt tatattcccc ggtcgatctt ttgctgacgg ttacaccgct
     5341 tactaccgat gtaatggtac tcaggaagca tctcttactg ttaaagataa caaactaagt
     5401 atggttgtag gtctttctaa gtttaatagg caactggata cttatagatt caaagaaaag
     5461 ggaatcgaag tagtaatgca gagagctgct tctgatgata actctgaggc cataatgtct
     5521 tttattatgc ctgacgttaa caatatgacc ttattagtta aaaataacgg ctacattatc
     5581 gttaagacaa cctgct
//
