LOCUS       NZ_CP034068             8401 bp    DNA     circular CON 16-APR-2024
DEFINITION  Shigella sonnei strain FDAARGOS_524 plasmid unnamed2, complete
            sequence.
ACCESSION   NZ_CP034068
VERSION     NZ_CP034068.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN10163218
            Assembly: GCF_003855115.1
KEYWORDS    RefSeq.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8401)
  AUTHORS   Campos,J., Goldberg,B., Tallon,L., Sadzewicz,L., Zhao,X.,
            Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S., Geyer,C.,
            Nandy,P., Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8401)
  AUTHORS   Campos,J., Goldberg,B., Tallon,L., Sadzewicz,L., Zhao,X.,
            Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S., Geyer,C.,
            Nandy,P., Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2018) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, Silver
            Spring, MD 20993-0002, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SMRT v. 2.3.0, HGAP v. 3.0, SPAdes v. 3.6.0
            Genome Coverage                   :: 1000.9613794x
            Sequencing Technology             :: PacBio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003855115.1-RS_2024_04_16
            Annotation Date                   :: 04/16/2024 11:34:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,040
            CDSs (total)                      :: 4,907
            Genes (coding)                    :: 4,266
            CDSs (with protein)               :: 4,266
            Genes (RNA)                       :: 133
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 100
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 641
            CDSs (without protein)            :: 641
            Pseudo Genes (ambiguous residues) :: 0 of 641
            Pseudo Genes (frameshifted)       :: 184 of 641
            Pseudo Genes (incomplete)         :: 434 of 641
            Pseudo Genes (internal stop)      :: 110 of 641
            Pseudo Genes (multiple problems)  :: 84 of 641
            Pseudo Genes (short protein)      :: 3 of 641
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP034068.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8401
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_524"
                     /isolation_source="Stool"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_524"
                     /db_xref="taxon:624"
                     /plasmid="unnamed2"
                     /collection_date="2015-12-29"
                     /collected_by="Children's National Hospital"
     gene            complement(29..679)
                     /gene="tetR(A)"
                     /locus_tag="EGX92_RS25305"
                     /old_locus_tag="EGX92_25350"
     CDS             complement(29..679)
                     /gene="tetR(A)"
                     /locus_tag="EGX92_RS25305"
                     /old_locus_tag="EGX92_25350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional repressor
                     TetR(A)"
                     /protein_id="WP_000164043.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
                     /name="tetR(A)"
     gene            785..1984
                     /gene="tet(A)"
                     /locus_tag="EGX92_RS25310"
                     /old_locus_tag="EGX92_25355"
     ARG             786..1981
                     /name="tet(A)"
                     /gene="tet(A)"
                     /gene_name="tetracycline efflux MFS transporter Tet(A)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(<1951..2139)
                     /locus_tag="EGX92_RS25315"
                     /old_locus_tag="EGX92_25360"
                     /pseudo=""
     CDS             complement(<1951..2139)
                     /locus_tag="EGX92_RS25315"
                     /old_locus_tag="EGX92_25360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /name="IS91 family transposase"
     gene            complement(2183..2572)
                     /locus_tag="EGX92_RS25320"
                     /old_locus_tag="EGX92_25365"
     CDS             complement(2183..2572)
                     /locus_tag="EGX92_RS25320"
                     /old_locus_tag="EGX92_25365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000102614.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGHD
                     NSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANNVR
                     VGVTGTGWQCLATAGISAEGKRTFR"
                     /name="hypothetical protein"
     gene            2790..3230
                     /locus_tag="EGX92_RS25325"
                     /old_locus_tag="EGX92_25370"
     CDS             2790..3230
                     /locus_tag="EGX92_RS25325"
                     /old_locus_tag="EGX92_25370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005119689.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETSE
                     LIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMARE
                     AENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
                     /name="hypothetical protein"
     gene            complement(3997..4146)
                     /locus_tag="EGX92_RS26105"
     CDS             complement(3997..4146)
                     /locus_tag="EGX92_RS26105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001138880.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPVG
                     REAL"
                     /name="hypothetical protein"
     gene            4717..4875
                     /locus_tag="EGX92_RS26290"
                     /old_locus_tag="EGX92_25380"
     CDS             4717..4875
                     /locus_tag="EGX92_RS26290"
                     /old_locus_tag="EGX92_25380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032287700.1"
                     /translation="MHEPPVQSDRCALSGNYQLESNPERHDKTPLAAAISNRSAREDRL
                     RGLEVEA"
                     /name="hypothetical protein"
     gene            5335..6150
                     /gene="sul2"
                     /locus_tag="EGX92_RS25345"
                     /old_locus_tag="EGX92_25390"
     ARG             5336..6147
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            6211..7014
                     /gene="aph(3'')-Ib"
                     /locus_tag="EGX92_RS25350"
                     /old_locus_tag="EGX92_25395"
     ARG             6212..7011
                     /name="aph(3'')-Ib"
                     /gene="aph(3'')-Ib"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3'')-Ib"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            7014..7850
                     /locus_tag="EGX92_RS25355"
                     /old_locus_tag="EGX92_25400"
     CDS             7014..7850
                     /locus_tag="EGX92_RS25355"
                     /old_locus_tag="EGX92_25400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="WP_000480968.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
                     /name="aminoglycoside O-phosphotransferase APH(6)-Id"
     gene            8156..8398
                     /locus_tag="EGX92_RS25365"
                     /old_locus_tag="EGX92_25410"
     CDS             8156..8398
                     /locus_tag="EGX92_RS25365"
                     /old_locus_tag="EGX92_25410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="WP_000844627.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERGR
                     EAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
                     /name="transposase"
     ARG             7015..7847
                     /name="aph(6)-Id"
                     /gene="aph(6)-Id"
                     /gene_name="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(4182..4355)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.429"
                     /coverage="100.0"
                     /evalue="1.19e-84"
     Typing          4279..4884
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.593"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 gtgggttgcg ctccggcagc ggtcctgatc aatcgtcacc ctttctcggt ccttcaacgt
       61 tcctgacaac gagcctcctt ttcgccaatc catcgacaat caccgcgagt ccctgctcga
      121 acgctgcgtc cggaccggct tcgtcgaagg cgtctatcgc ggcccgcaac agcggcgaga
      181 gcggagcctg ttcaacggtg ccgccgcgct cgccggcatc gctgtcgccg gcctgctcct
      241 caagcacggc cccaacagtg aagtagctga ttgtcatcag cgcattgacg gcgtccccgg
      301 ccgaaaaacc cgcctcgcag aggaagcgaa gctgcgcgtc ggccgtttcc atctgcggtg
      361 cgcccggtcg cgtgccggca tggatgcgcg cgccatcgcg gtaggcgagc agcgcctgcc
      421 tgaagctgcg ggcattcccg atcagaaatg agcgccagtc gtcgtcggct ctcggcaccg
      481 aatgcgtatg attctccgcc agcatggctt cggccagtgc gtcgagcagc gcccgcttgt
      541 tcctgaagtg ccagtaaagc gccggctgct gaacccccaa ccgttccgcc agtttgcgtg
      601 tcgtcagacc gtctacgccg acctcgttca acaggtccag ggcggcacgg atcactgtat
      661 tcggctgcaa ctttgtcatg cttgacactt tatcactgat aaacataata tgtccaccaa
      721 cttatcagtg ataaagaatc cgcgcgttca atcggaccag cggaggctgg tccggaggcc
      781 agacgtgaaa cccaacagac ccctgatcgt aattctgagc actgtcgcgc tcgacgctgt
      841 cggcatcggc ctgattatgc cggtgctgcc gggcctcctg cgcgatctgg ttcactcgaa
      901 cgacgtcacc gcccactatg gcattctgct ggcgctgtat gcgttgatgc aatttgcctg
      961 cgcacctgtg ctgggcgcgc tgtcggatcg tttcgggcgg cggccggtct tgctcgtctc
     1021 gctggccggc gctgctgtcg actacgccat catggcgacg gcgcctttcc tttgggttct
     1081 ctatatcggg cggatcgtgg ccggcatcac cggggcgact ggggcggtag ccggcgctta
     1141 tattgccgat atcactgatg gcgatgagcg cgcgcggcac ttcggcttca tgagcgcctg
     1201 tttcgggttc gggatggtcg cgggacctgt gctcggtggg ctgatgggcg gtttctcccc
     1261 ccacgctccg ttcttcgccg cggcagcctt gaacggcctc aatttcctga cgggctgttt
     1321 ccttttgccg gagtcgcaca aaggcgaacg ccggccgtta cgccgggagg ctctcaaccc
     1381 gctcgcttcg ttccggtggg cccggggcat gaccgtcgtc gccgccctga tggcggtctt
     1441 cttcatcatg caacttgtcg gacaggtgcc ggccgcgctt tgggtcattt tcggcgagga
     1501 tcgctttcac tgggacgcga ccacgatcgg catttcgctt gccgcatttg gcattctgca
     1561 ttcactcgcc caggcaatga tcaccggccc tgtagccgcc cggctcggcg aaaggcgggc
     1621 actcatgctc ggaatgattg ccgacggcac aggctacatc ctgcttgcct tcgcgacacg
     1681 gggatggatg gcgttcccga tcatggtcct gcttgcttcg ggtggcatcg gaatgccggc
     1741 gctgcaagca atgttgtcca ggcaggtgga tgaggaacgt caggggcagc tgcaaggctc
     1801 actggcggcg ctcaccagcc tgacctcgat cgtcggaccc ctcctcttca cggcgatcta
     1861 tgcggcttct ataacaacgt ggaacgggtg ggcatggatt gcaggcgctg ccctctactt
     1921 gctctgcctg ccggcgctgc gtcgcgggct ttggagaaat tcttcaaatt cccgttgcac
     1981 atagcccggc aattcctttc cctgctctgc cataagcgca gcgaatgccg ggtaatactc
     2041 gtcaacgatc tgatagagaa gggtttgctc gggtcggtgg ctctggtaac gaccagtatc
     2101 ccgatcccgg ctggccgtcc tggccgccac atgaggcatg ttccgcgtcc ttgcaatact
     2161 gtgtttacat acagtctatc gcttagcgga aagttctttt accctcagcc gaaatgcctg
     2221 ccgttgctag acattgccag ccagtgcccg tcactcccac tcttacattg ttcgcagtat
     2281 ctaggtacat gttattgcgt gcaccttcat gatgtataat gttattaatg acagcagaaa
     2341 gatcgacaga attaatattc ttcgtacctg ggacagttat cgggtgtcgg ccagtgatga
     2401 gaccaaagga tgcagtataa gatgaaccac tactattatc atggccacca ttaccaccac
     2461 cgttaaaact acctcccgaa cttccagagc cgctatgacc atcattgtag gggtcatttg
     2521 gtcccatgtg accaaaacca ccgacagtac cgctatttaa ctctgtactc ataaaattat
     2581 cctctttgac ttttaaaaac agcaaaaact gtatttataa caatgtgttt atatacagta
     2641 ttcgaaatac tacgcccgtc aaaaccacac cgtcaacagg ttttttgtgt atttttcacg
     2701 cctcacgggc agcctcagac cttttgtgtt aaaacagtaa tatgttctgc tgttacaata
     2761 cggtacacac aggagacact gtcactttta tggcatggca cacagttcaa gagacactga
     2821 aattaacagg aaagtcgcgc tcgcaattat atcgtgacat ggctaaaggc cttgtcagct
     2881 atcgcacagg aaaaaatgat cgccgggagt tcgagaccag tgaactgatt cgggcctatg
     2941 gcgagctgcg cacttctgag acaccaaaaa ggcacaagga gggacactcc aaatctgtcg
     3001 ttagccaggc ggaaaatgct cagctagacg tcattcagca gaaactggac gctctgcagc
     3061 agactgttac cctaatgctt gaagacaaga tggcgcggga agcagaaaac cgcctgcgaa
     3121 atgaggagag ggaacaacta caggccgaaa ttagccgtct ggctgacgcg ctggcacagg
     3181 aaaaaaagcg gggattctgg tcgcggctgt ttcgctccgg ttcagagtaa cagtgcaggt
     3241 gactctgatt taccataaca tctgttatca gcaccgcctg agaggccctc agggcgtttc
     3301 tgacgggaaa ccggtaaaac aggcaacaaa ccacccgaaa acccgccaga aacgcgcagg
     3361 ctctgttttt acggcatgca tgagtatgcg cacaagtgca tagcgcggaa attcacacca
     3421 ttcagccggg gtaagtcctg tttccggcgt tcttcccatc cgaaaacggt gaacggggac
     3481 gcaatttatt gcgtggccgt gaagcgttgc cggcgcagcc ggaaaacccg ctgtcagcac
     3541 gttcaggaag ggggtgatgt acaaatggca cagaggatga aagtgacgta tgagctgaag
     3601 gaatgatgta aaagtgatgt ataagtgatg taaaagtgat gtataagtga tgtaaaagtg
     3661 atgtatgaac tgaaggagcg atgtaattct gtagtcagga agaataaccc gttcatgcag
     3721 gcatgaacga tacagtgagg aacgcttttt ttgtcacgat ctccccttaa cctgcgtaaa
     3781 tcacccgcca tggttgatta tttttcaacc tcaaaatggt catacaccta acgcagccgc
     3841 gattttcatc gcaactgcat gaattaaaag aaaaaaaagg aatatttcgt tttcacgagt
     3901 aatacccggg tattaaaaat ccactgtaat tacatgaaaa taaagaaata aaaggtaata
     3961 ccggagtatt accacgtacg cgggcaagcg aaaaagctat aacgcttccc ggccgacagg
     4021 cgacccaaaa tccacagggg catgcaggtt ctcttcagta acctgcgcca ttaaatcaaa
     4081 aaggtcatat tcgccgggaa gctgcgtatc agtcgaatcc agttgttcat catattcaga
     4141 tggcatgggg tgatatccag tagctgagag gttgttagcg ggtttcgggg cgcagccctg
     4201 aaccagtcat gtagcgctag cggagtgtat actggcttac tatgttgcca cgattgcgaa
     4261 aatcagtgaa gtgcttcatg tggcaggaga aaaaaggcag caccggcgcg tcagcagaat
     4321 atgcgataca ggagatattc cgcttcctcg ctcactgact cgctacgctc ggtcgttcgg
     4381 ctgcggcgag cggtaccggc ttacggacgg ggacggaaat ttcctggaag ataccaggaa
     4441 gacacttagc aaagaagaga taaggccacg gcgaagccgt ttttccatag gctccgcccc
     4501 cctgacaagc atcgcaaaat ctgacgctca aatcagtggt ggcgaaaccc gacaggacta
     4561 taaagatccc aggcgtttcc ccctggtagc tccctcgtgc gctctcctgt tcctgccttt
     4621 cggtttaccg gtgtcattcc gctgttatgg ccgcggtttc tcattccacg cctgacactc
     4681 agttccgtga aggcagttcg ctccaagctg gactgtatgc acgaaccccc cgttcagtcc
     4741 gaccgctgcg ccttgtccgg taactatcaa cttgagtcca acccggaaag acacgacaaa
     4801 acgccactgg cagcagccat tagtaacagg agtgcacgag aagatagatt aagaggtctt
     4861 gaagtagagg cctgactgcg gctacactga aagaacagtt ttggtaatgg cggtctcgtg
     4921 aaccctgtta ccgggttaag cagttcccca actgacttaa ccttcgaaca aaccgcctcc
     4981 ccaggcggtt ttttcgttta cagagcaaga gattacgatt cattgaaaag gatctcaaga
     5041 agatcctttg tagattacta tcaattaatc ttccgaacat gacaccagtc actgttgatt
     5101 acggttaaac cgctccgtaa caggtttccc tcgtattggt gacttgcagc accaaagaag
     5161 caaaaaagat aaatatatct acttattgag agatgatata tttatttcca aaatgctaca
     5221 gatacatcat catgaaacgg atcaccgcaa acaggttact cgcctgggga ttccctttcg
     5281 acccgagcat ccgtatgaga ctcatgctcg attattatta ttatagaagc ccccatgaat
     5341 aaatcgctca tcattttcgg catcgtcaac ataacctcgg acagtttctc cgatggaggc
     5401 cggtatctgg cgccagacgc agccattgcg caggcgcgta agctgatggc cgagggggca
     5461 gatgtgatcg acctcggtcc ggcatccagc aatcccgacg ccgcgcctgt ttcgtccgac
     5521 acagaaatcg cgcgtatcgc gccggtgctg gacgcgctca aggcagatgg cattcccgtc
     5581 tcgctcgaca gttatcaacc cgcgacgcaa gcctatgcct tgtcgcgtgg tgtggcctat
     5641 ctcaatgata ttcgcggttt tccagacgct gcgttctatc cgcaattggc gaaatcatct
     5701 gccaaactcg tcgttatgca ttcggtgcaa gacgggcagg cagatcggcg cgaggcaccc
     5761 gctggcgaca tcatggatca cattgcggcg ttctttgacg cgcgcatcgc ggcgctgacg
     5821 ggtgccggta tcaaacgcaa ccgccttgtc cttgatcccg gcatggggtt ttttctgggg
     5881 gctgctcccg aaacctcgct ctcggtgctg gcgcggttcg atgaattgcg gctgcgcttc
     5941 gatttgccgg tgcttctgtc tgtttcgcgc aaatcctttc tgcgcgcgct cacaggccgt
     6001 ggtccggggg atgtcggggc cgcgacactc gctgcagagc ttgccgccgc cgcaggtgga
     6061 gctgacttca tccgcacaca cgagccgcgc cccttgcgcg acgggctggc ggtattggcg
     6121 gcgctgaaag aaaccgcaag aattcgttaa ctgcacattc gggatatttc tctatattcg
     6181 cgcttcatca gaaaactgaa ggaacctcca ttgaatcgaa ctaatatttt ttttggtgaa
     6241 tcgcattctg actggttgcc tgtcagaggc ggagaatctg gtgattttgt ttttcgacgt
     6301 ggtgacgggc atgccttcgc gaaaatcgca cctgcttccc gccgcggtga gctcgctgga
     6361 gagcgtgacc gcctcatttg gctcaaaggt cgaggtgtgg cttgccccga ggtcatcaac
     6421 tggcaggagg aacaggaggg tgcatgcttg gtgataacgg caattccggg agtaccggcg
     6481 gctgatctgt ctggagcgga tttgctcaaa gcgtggccgt caatggggca gcaacttggc
     6541 gctgttcaca gcctatcggt tgatcaatgt ccgtttgagc gcaggctgtc gcgaatgttc
     6601 ggacgcgccg ttgatgtggt gtcccgcaat gccgtcaatc ccgacttctt accggacgag
     6661 gacaagagta cgccgcagct cgatcttttg gctcgtgtcg aacgagagct accggtgcgg
     6721 ctcgaccaag agcgcaccga tatggttgtt tgccatggtg atccctgcat gccgaacttc
     6781 atggtggacc ctaaaactct tcaatgcacg ggtctgatcg accttgggcg gctcggaaca
     6841 gcagatcgct atgccgattt ggcactcatg attgctaacg ccgaagagaa ctgggcagcg
     6901 ccagatgaag cagagcgcgc cttcgctgtc ctattcaatg tattggggat cgaagccccc
     6961 gaccgcgaac gccttgcctt ctatctgcga ttggaccctc tgacttgggg ttgatgttca
     7021 tgccgcctgt ttttcctgct cattggcacg tttcgcaacc tgttctcatt gcggacacct
     7081 tttccagcct cgtttggaaa gtttcattgc cagacgggac tcctgcaatc gtcaagggat
     7141 tgaaacctat agaagacatt gctgatgaac tgcgcggggc cgactatctg gtatggcgca
     7201 atgggagggg agcagtccgg ttgctcggtc gtgagaacaa tctgatgttg ctcgaatatg
     7261 ccggggagcg aatgctctct cacatcgttg ccgagcacgg cgactaccag gcgaccgaaa
     7321 ttgcagcgga actaatggcg aagctgtatg ccgcatctga ggaacccctg ccttctgccc
     7381 ttctcccgat ccgggatcgc tttgcagctt tgtttcagcg ggcgcgcgat gatcaaaacg
     7441 caggttgtca aactgactac gtccacgcgg cgattatagc cgatcaaatg atgagcaatg
     7501 cctcggaact gcgtgggcta catggcgatc tgcatcatga aaacatcatg ttctccagtc
     7561 gcggctggct ggtgatagat cccgtcggtc tggtcggtga agtgggcttt ggcgccgcca
     7621 atatgttcta cgatccggct gacagagacg acctttgtct cgatcctaga cgcattgcac
     7681 agatggcgga cgcattctct cgtgcgctgg acgtcgatcc gcgtcgcctg ctcgaccagg
     7741 cgtacgctta tgggtgcctt tccgcagctt ggaacgcgga tggagaagag gagcaacgcg
     7801 atctagctat cgcggccgcg atcaagcagg tgcgacagac gtcatactag atatcaagcg
     7861 acttctccta tcccctggga acacatcaat ctcaccggag aatatcgctg gccaaagcct
     7921 tagcgtagga ttccgcccct tcccgcaaac gacccctgga agccccgtag cgacgcggag
     7981 aggggcgaga caagccaagg gcgcaggctc gatgcgcagc acgacatagc cggttctcgc
     8041 aaggacgaga atttccctgc ggtgcccctc aagtgtcaat gaaagtttcc aacgcgagcc
     8101 attcgcgaga gccttgagtc cacgctagat ctatctcatc tgcgcaaggc agaacgtgaa
     8161 gacggccgcc ctggacctcg cccgcgagcg ccaggcgcac gaggccggcg cgcggacccg
     8221 cgccacggcc cacgagcgga cgccgcagca ggagcgccag aaggccgcca gagaggccga
     8281 gcgcggccgt gaggcttgga cgctagggca gggcatgaaa aagcccgtag cgggctgcta
     8341 cgggcgtctg acgcggtgga aagggggagg ggatgttgtc tacatggctc tgctgtagtg
     8401 a
//
