LOCUS       NZ_CP034051             3181 bp    DNA     circular CON 26-NOV-2023
DEFINITION  Klebsiella pneumoniae strain KP_NORM_BLD_2014_104014 plasmid
            unnamed6, complete sequence.
ACCESSION   NZ_CP034051
VERSION     NZ_CP034051.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA5063299
            Assembly: GCF_003855315.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3181)
  AUTHORS   Lam,M.C., Wyres,K.L., Wick,R., Judd,L.M., Fostervold,A., Holt,K.E.
            and Lohr,I.H.
  TITLE     Convergence of virulence and multidrug resistance in a single
            plasmid vector in multidrug-resistant Klebsiella pneumoniae ST15
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3181)
  AUTHORS   Lam,M.C., Wyres,K.L., Wick,R., Judd,L.M., Fostervold,A., Holt,K.E.
            and Lohr,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-NOV-2018) Biochemistry and Molecular Biology,
            University of Melbourne, Flemington Road, Parkville, Victoria 3010,
            Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.6.2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 250x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/26/2023 02:47:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,772
            CDSs (total)                      :: 5,646
            Genes (coding)                    :: 5,482
            CDSs (with protein)               :: 5,482
            Genes (RNA)                       :: 126
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 164
            CDSs (without protein)            :: 164
            Pseudo Genes (ambiguous residues) :: 0 of 164
            Pseudo Genes (frameshifted)       :: 67 of 164
            Pseudo Genes (incomplete)         :: 96 of 164
            Pseudo Genes (internal stop)      :: 32 of 164
            Pseudo Genes (multiple problems)  :: 28 of 164
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP034051.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3181
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP_NORM_BLD_2014_104014"
                     /isolation_source="Blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed6"
                     /geo_loc_name="Norway"
                     /collection_date="2014"
     gene            268..459
                     /locus_tag="EHF42_RS29575"
                     /old_locus_tag="EHF42_29575"
     CDS             268..459
                     /locus_tag="EHF42_RS29575"
                     /old_locus_tag="EHF42_29575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041614.1"
                     /translation="MNKQQKTAVNMAKFIQDQSLLLLDRLNELDLDHEADFCEKLHEDA
                     ERLYRSLSARLNEQQDGA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1315..1587
                     /locus_tag="EHF42_RS29580"
                     /old_locus_tag="EHF42_29580"
     CDS             1315..1587
                     /locus_tag="EHF42_RS29580"
                     /old_locus_tag="EHF42_29580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032725101.1"
                     /translation="MATDAQIKQINALIKKYPDSCSICQQDYDEDYVTYTVFGYDKNRK
                     MQVTTGCCARTLTELVLLGVCGCFDPDERDEIMKNHPMAKKFFTN"
                     /name="hypothetical protein"
     gene            1700..2026
                     /locus_tag="EHF42_RS29585"
                     /old_locus_tag="EHF42_29585"
     CDS             1700..2026
                     /locus_tag="EHF42_RS29585"
                     /old_locus_tag="EHF42_29585"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032431019.1"
                     /translation="MFGFFKKKKLYEEICKDAGMALSDGLLAQGLARNKIEAMGAGAVF
                     SQSLREAVSQGYKSSDAIAEARKNTSHHLAARGFDFETIASAIDVFCTATAFESMLDLA
                     RDKG"
                     /name="hypothetical protein"
     gene            complement(2919..3032)
                     /locus_tag="EHF42_RS29590"
                     /old_locus_tag="EHF42_29590"
     CDS             complement(2919..3032)
                     /locus_tag="EHF42_RS29590"
                     /old_locus_tag="EHF42_29590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_072003743.1"
                     /translation="MHEPPVQSDYHARSGNYRLESNPERHDKSPLAVATGN"
                     /name="replication initiation protein"
     Typing          complement(3..393)
                     /name="rep_cluster_2327"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.341"
                     /coverage="99.0"
                     /evalue="9.91e-116"
     Typing          complement(2916..3181)
                     /name="rep_cluster_2373"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.382"
                     /coverage="82.0"
                     /evalue="5.26e-112"
ORIGIN
        1 gatctttaag tcctgtcggg tttcgccacc actgatttga gcgtcagatt tcgtgatgct
       61 tgtcaggggg gcggagccta tggaaaaacg cccgccgggg cgtggccttt tcgcccttgt
      121 ccttagcttt cgttatgctt accgttacgt tatctgctta tcctccctcc gctcgccgca
      181 gactcatgac cgaacggagt gagcgaatga gcgaggaagc ggaaaacaat cagaacgtac
      241 agtaagcact ttcgcgccgg ggccttcatg aacaaacaac aaaagacagc cgttaacatg
      301 gctaaattca tccaggatca gtccctgctt cttctggaca ggcttaatga gctggatctg
      361 gatcatgagg ctgatttctg cgagaaactc catgaggatg cggaacgcct ttaccgaagc
      421 ctgtcagcgc gcctgaatga gcagcaggac ggtgcgtaat gtctccgttc tgggtattac
      481 cggagtaata ccggcatcgc cttaattttc attgtcttag cgcttaacat tattaccccg
      541 gtaataccca caatccctgt attttttctt ttttatttat ttttcatgaa gttgcgatga
      601 aaatcgcagc tgcgttaggt gtatgaccat tttgaggttg aaaaataatc aactatggcg
      661 ggtgatttac gcaggttaag ggggatcgtg acaaaaaaag cgttcctcac tgtatcgttc
      721 atgcctgcat gaacgggtta ttcttcctga ctacagaatt acatcgctcc ttcagttcat
      781 acatcacttc tacaacactt ttacaacact tttacatcat tccttcagcc aatacatcac
      841 ttttacatca cttttgcatc actcttacaa cacttttaca tcattccttc agctcatacg
      901 tcacttttac gtcagttcca tcctctgtgc catttgtaca tcacgccctt cctgaacgtg
      961 ctgacagcgg gttttccggc tgcgccggca acgcctcacg gccacgcaat aaattgcgca
     1021 tccgttcacc gttatcgggg tggtttgtgg tggctttcag ggcgttcatc ggattttagc
     1081 ggctaaaatc ccggccaggt atgtccgtgc atagccatgc agtcgcgcca gaatcatcag
     1141 gggcgttttt tctcgatatg ggcacagaaa ggtgtgtgtt tcaggccgga ggccgcagaa
     1201 acgcgctgag ggcgtctcag acggtgcgga caagtagtct tatgttaaat cagctatggg
     1261 attttctggt gtggtacatt caatgccagt tatggatgta ctaacggaga acttgtggct
     1321 actgatgcac aaataaaaca gattaacgct ctcatcaaaa agtatcctga ctcgtgctct
     1381 atttgccagc aggattatga tgaggattac gtgacgtata cggtttttgg atacgacaaa
     1441 aaccggaaga tgcaggtaac aacgggttgt tgcgctcgaa cgcttacaga actggtcttg
     1501 ctgggcgtct gcggatgctt tgatcctgat gaacgtgatg agatcatgaa aaatcaccca
     1561 atggcgaaga agttcttcac aaactaaata ctggaaaatt attgctcaag cccgcaattt
     1621 gcgggctttt tcatgcgctg cggtaaaggg atgttatggt aaattgctca tcacagtagc
     1681 cagttaagca gaggttagaa tgtttgggtt ttttaagaaa aaaaagctct atgaagagat
     1741 ctgtaaagat gcagggatgg ctttatctga tgggttattg gcccaaggct tagcgagaaa
     1801 caaaatagaa gcaatggggg ctggtgctgt ttttagtcag tctttgaggg aggctgtttc
     1861 tcaaggttac aagtcttctg atgcgattgc tgaagcaaga aaaaacacat ctcatcatct
     1921 agctgctaga gggtttgatt ttgaaacgat cgcaagtgcg attgatgttt tttgtactgc
     1981 tacagcattt gaatcaatgt tagatcttgc tcgcgataaa ggctgaatat cgagaggagt
     2041 gacgggttgt tatcattagc ctcagagtag atttgaggcc ttttgggcgg tgtatgcagg
     2101 agatgttatg gtaaatatgt cttattgaga atattcttac taatgcgcgg tttttttcta
     2161 atttttaaat ggatgactta aaaatgaagg caagattttt tgcagccttg cttattactt
     2221 tttttctttc tggatgtgcg acagaggcgg gatatcaaag ggtattaaat tcatggatgg
     2281 gatctactga tgtgagcttg attcagtcat gggggccgcc tcagcaatct tatgaacttt
     2341 ctggccatac cttcctagtt tattcaaata gctctagctc ttatatacct ggagtggctc
     2401 ctacctatca gactacgttt attggtaata cagcgtatac aaacacctat ggcggctcgc
     2461 ccggctttaa tgttagctac tcatgtacta cgacttttga ggttgttggc gggaaaattg
     2521 ttaactggcg ttatcagggc aacaattgca catcgcgtta gtcgtgtgtc caatcgtagt
     2581 atcaggtgaa gatacaatag agaacttaat accctcttgg tgggtatata aaaaccaaga
     2641 tgagcgtagc gaccttcttt aaactcgttc cttgtgtgtc ttaccctgca aaaggtttga
     2701 ttgtgtgggt gtaaatgaaa tcgttgcgtt gacagttaca tgtgacgaga tgtagtctct
     2761 aaaacgaaaa aaccgccctg caaggcggtt ttttcgaagg ttctctgagc taccaactct
     2821 ttgaaccgag gtaactggct tggaggagcg cagtcaccaa aactgttcgt ttagtttagc
     2881 cttaaccggc gcataacttc aagactatct cctctaaatc agttaccagt ggctaccgcc
     2941 aatggcgatt tgtcgtgtct ttccgggttg gactcaagac gatagttacc ggaacgggcg
     3001 tggtagtcgg actgaacggg gggttcgtgc atacagtcca gcttggagcg aactgcctac
     3061 ccggaactga gtgtcaggcg tggaatgaga taaacgcggc cataacagcg gaatgacacc
     3121 ggtaaaccga aaggcaggaa caggagagcg cacgagggag ccaccagggg gaaacgcctg
     3181 g
//
