LOCUS       NZ_CP034050             4591 bp    DNA     circular CON 26-NOV-2023
DEFINITION  Klebsiella pneumoniae strain KP_NORM_BLD_2014_104014 plasmid
            unnamed5, complete sequence.
ACCESSION   NZ_CP034050
VERSION     NZ_CP034050.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA5063299
            Assembly: GCF_003855315.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4591)
  AUTHORS   Lam,M.C., Wyres,K.L., Wick,R., Judd,L.M., Fostervold,A., Holt,K.E.
            and Lohr,I.H.
  TITLE     Convergence of virulence and multidrug resistance in a single
            plasmid vector in multidrug-resistant Klebsiella pneumoniae ST15
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4591)
  AUTHORS   Lam,M.C., Wyres,K.L., Wick,R., Judd,L.M., Fostervold,A., Holt,K.E.
            and Lohr,I.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-NOV-2018) Biochemistry and Molecular Biology,
            University of Melbourne, Flemington Road, Parkville, Victoria 3010,
            Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.6.2
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 250x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/26/2023 02:47:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,772
            CDSs (total)                      :: 5,646
            Genes (coding)                    :: 5,482
            CDSs (with protein)               :: 5,482
            Genes (RNA)                       :: 126
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 164
            CDSs (without protein)            :: 164
            Pseudo Genes (ambiguous residues) :: 0 of 164
            Pseudo Genes (frameshifted)       :: 67 of 164
            Pseudo Genes (incomplete)         :: 96 of 164
            Pseudo Genes (internal stop)      :: 32 of 164
            Pseudo Genes (multiple problems)  :: 28 of 164
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP034050.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4591
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP_NORM_BLD_2014_104014"
                     /isolation_source="Blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed5"
                     /geo_loc_name="Norway"
                     /collection_date="2014"
     gene            277..468
                     /locus_tag="EHF42_RS29535"
                     /old_locus_tag="EHF42_29535"
     CDS             277..468
                     /locus_tag="EHF42_RS29535"
                     /old_locus_tag="EHF42_29535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_016947206.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLSLRLDALQEGD"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            468..866
                     /locus_tag="EHF42_RS29540"
                     /old_locus_tag="EHF42_29540"
     CDS             468..866
                     /locus_tag="EHF42_RS29540"
                     /old_locus_tag="EHF42_29540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016162088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_117040471.1"
                     /translation="MANVSISEAARLTGKSRTTLHRLIKAGELSTCRGERNAKMLETSE
                     LLRVFGAFAPVSVEQAEEQVSGQRDTVTSDRNEQVIYILKQEIEHLNTLLASKDSHIDS
                     LKQAMLLLENREDQRQNTTRPWWRFWQS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(873..1571)
                     /locus_tag="EHF42_RS29545"
                     /old_locus_tag="EHF42_29545"
     CDS             complement(873..1571)
                     /locus_tag="EHF42_RS29545"
                     /old_locus_tag="EHF42_29545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016947204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_048290303.1"
                     /translation="MTRTFILLCTGVFAIAYALYELNHYGVYYPDVSNPEAFTGWKAVT
                     ELGPAILATGIAATAFSLWLFILFFRGSESRRIQREISGYQQALREAEEAHRARQNTLI
                     QNHNLETAALRSHYIGAEDVLREAFAQKESLLLQAVQNARKEARKELEEQREALNQRES
                     ILQKKELALETTLEKARKYVAGERHNTELARLEAEKAEERRRNACATAERRRRKLEKLQ
                     ATGGTAGQKH"
                     /name="hypothetical protein"
     gene            complement(1568..2455)
                     /locus_tag="EHF42_RS29550"
                     /old_locus_tag="EHF42_29550"
     CDS             complement(1568..2455)
                     /locus_tag="EHF42_RS29550"
                     /old_locus_tag="EHF42_29550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016947203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="WP_117040472.1"
                     /translation="MAKFASAARQAAGVIKPLQGNILRSVGTVRNYEQGLTRVAEWMKA
                     NRINHGDLRTLTPAQAITYLEQRGEQVGQKTLDMERQAIQAMMIHVTQTLPAGEHLPVV
                     KSEQAQILSSRAYSPDQVALIAAAQQPANALATAIALAAGLRAHELLTLAPRQERAPDP
                     RPAADSKFAGREGVIYTVQGKGGLVREVLIPSPLAERLEHVRLASPVRVTDRGVFYQSQ
                     YAIGGGQRWSNAFSAASMRTLGWSRGAHGVRHSYAQQRMQELQKLGLVRDMALRTVSQE
                     MGHFRPEITETYLR"
                     /name="site-specific integrase"
     gene            complement(2448..2669)
                     /locus_tag="EHF42_RS29555"
                     /old_locus_tag="EHF42_29555"
     CDS             complement(2448..2669)
                     /locus_tag="EHF42_RS29555"
                     /old_locus_tag="EHF42_29555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016162091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016947202.1"
                     /translation="MQFDTAAELAALQAQTRRIRQVRYRPSRLDRYTGELLSLYQAGAS
                     AAELQRWLRARRIKVVLSTVTRWLEKNG"
                     /name="hypothetical protein"
     gene            3250..3600
                     /locus_tag="EHF42_RS29560"
                     /old_locus_tag="EHF42_29560"
     CDS             3250..3600
                     /locus_tag="EHF42_RS29560"
                     /old_locus_tag="EHF42_29560"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_117040473.1"
                     /translation="MQVGESVVNAIVEYHAQGSEEAHAEENRMFLRYLHELTGTQRVAV
                     EVARKYFSPVRADMSYLFLVAGTAPGATVLLQNCPHLTGLDGPFFYAFNVRGPFLGRNH
                     RLCGGVHSHAYA"
                     /name="hypothetical protein"
     gene            complement(4329..4442)
                     /locus_tag="EHF42_RS29565"
                     /old_locus_tag="EHF42_29565"
     CDS             complement(4329..4442)
                     /locus_tag="EHF42_RS29565"
                     /old_locus_tag="EHF42_29565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032634168.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHAKAPLAAATGN"
                     /name="hypothetical protein"
     Typing          complement(2..124)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="87.402"
                     /coverage="99.0"
                     /evalue="1.9900000000000003e-31"
     Typing          complement(4153..4591)
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="85.439"
                     /coverage="88.0"
                     /evalue="8.249999999999999e-126"
ORIGIN
        1 tatctttaaa gtcctgtcgg gtttcgccac cgctgatttg agcgtccgtt tctgtgatgc
       61 ttgtcagggg ggcggagcct atggaaaaac ggcaacgccg tggccttctc ccgtctgttc
      121 ttttcctttt ccttttccgg gtggtggcga tgacttttct gtctgtgtgc cccgtccgct
      181 cgccgcagcc gaacggcagg agcgtagcga ctgagtgagc gaggaagcgg aaaagagacc
      241 ggttttacac tgagcactga cgccctgagg ccccttatga cgaaacaaca gaaaactgcc
      301 ctgaacatgg ctaaattcat ccagaatcag tccctgctcc tgctggagaa gcttaacgag
      361 cttgatctcg atgcggaggc tgacctttgt gagaaactcc acgatgatgc ggagcatctt
      421 ttccgtacac tgtccctcag actggatgca ctacaggagg gtgactgatg gcgaacgtgt
      481 cgatcagtga ggctgcacgc ctgacgggta agagcaggac aaccctgcac aggttaatta
      541 aagccggtga actgtccacc tgtcggggtg aacggaatgc gaaaatgctc gagacttcag
      601 agcttttgcg ggtgtttggt gcctttgcgc cggtatccgt tgaacaggcg gaagaacagg
      661 tcagtggaca gcgtgacaca gtcacttctg accgtaatga acaggtcatt tacattctga
      721 aacaggaaat tgaacacctg aacacccttc ttgcctcaaa agattcacat atcgacagtc
      781 tgaaacaggc gatgttgttg ctggagaacc gggaagatca gcggcagaac acgacccggc
      841 cctggtggcg tttctggcag tcctgaaatg tctcagtgtt tttgccctgc tgttccgcca
      901 gtggcctgga gtttttccag tttccgtctg cgccgttccg ccgtcgcgca ggcatttctc
      961 cgtctttcct ctgctttctc tgcttccaga cgggccagct cggtgttatg ccgctccccg
     1021 gccacatatt tccgggcctt ctccagggtt gtttccagcg ccagctcctt tttctgcagg
     1081 atggattcgc gctgattcag tgcctccctc tgttcttcca gctcctttct ggcttctttc
     1141 cgggcattct gcaccgcctg aagcaagagg ctttcttttt gcgcaaacgc ttcccgcagt
     1201 acatcctcag caccaatgta gtggcttcgc agggccgctg tctccaggtt gtgattctga
     1261 ataagggtat tttgtctggc tctgtgagcc tcttctgcct ccctcagtgc ctgctgatat
     1321 ccgcttatct cgcgctgtat ccggcgggac tctgagcctc tgaaaaacaa aatgaacagc
     1381 cacagcgaga acgctgtggc tgcgatcccc gttgccagga tagcgggacc gagttcggtg
     1441 accgccttcc agccagtaaa cgcctcagga ttgctgacgt ccggatagta aacgccatag
     1501 tgattcagct catacagggc gtacgcgatg gcgaaaacac cggtacacaa taaaataaat
     1561 gttcttgtca tcgcaggtat gtctccgtga tttccggacg aaaatggccc atttcctggc
     1621 tgacggtgcg cagggccata tcacgaacca gaccgagttt ttgcagctcc tgcatccgtt
     1681 gctgggcgta gctgtgacgc acgccgtgtg ccccccgtga ccagcccagg gtacgcatgg
     1741 aggcggcact gaaagcgttc gaccagcgct gtcctccgcc aatggcgtac tgactctggt
     1801 aaaacacgcc gcgatcggtc acccggaccg ggctcgccag cctgacatgc tccagacgtt
     1861 cagccagggg ggacggtatc aggacttccc tgaccaggcc gcctttgccc tgaacggtgt
     1921 aaatcacacc ctcccgaccg gcaaatttac tgtccgctgc cggacgggga tccggtgctc
     1981 gctcctggcg gggcgcgagg gtcagcagtt catgggcgcg taatccggcg gccagggcaa
     2041 tcgcggtggc cagagcgttg gcgggctgct gtgcagcggc aatcagggcc acctggtccg
     2101 ggctgtaggc ccggctgctc aggatctgtg cctgctccga tttcaccacc ggcaggtgtt
     2161 cgccggcagg gagggtctgc gtcacgtgga tcatcatggc ctggatggcc tggcgttcca
     2221 tatcgagcgt tttctggcca acctgctccc cgcgctgctc caggtaggtg atggcctgcg
     2281 ccggtgtcag tgtgcgtaaa tcgccgtgat taatccggtt ggctttcatc cactctgcga
     2341 cgcgggtcag tccctgctcg taattccgga ctgtgccgac agagcgcaga atatttccct
     2401 ggagtggttt tatcacgcca gccgcctggc gggcagcgct ggcgaattta gccatttttt
     2461 tccagccagc gggtaaccgt ggacaacacc accttgattc gccgggcccg gagccagcgt
     2521 tgcagctccg ctgcactggc cccggcctgg tacaggctca gcagttcgcc ggtatagcgg
     2581 tcaaggcggg agggacggta gcggacctgg cggatacggc gggtctgcgc ctgcagggcg
     2641 gccagttcgg cagcggtatc aaattgcatc tgtgctccgg attgggtgta agaacatcag
     2701 tggccgttac gttaacggtc actgcggcat gcgccgatcg ccctcgttcg aacctctcgt
     2761 ggttcagccc ggactgctcg cggctgataa cgtcccttaa gccagggaat ggcctgacct
     2821 gattgccggt ctcgcagatt ttagctgttt tctcaggatt ctcggattgg gctgcccggt
     2881 acagtatccc cggagggacc cggctgtttt acctgctgcc ggtaggcagg tgcgcgtcag
     2941 agtgcgcctc ctcatggttt gactaaatac tagccaggtt tttcgtgcgt gtgaatgttg
     3001 ttttttaaat gttgcgcctg actccgctca atagcgacac ccgctatgta caggcgcatt
     3061 tatgcgtcgt acatagcggg tgtcgcaggt gctgatttgg cggaataaaa aacaccggga
     3121 ctgaccgccc cggaccccgc caacggtgac cggacagaca aaaaatttgc ctgcccggtc
     3181 accgtttcac atcatggcga ggaggcggga tctgaaacgg tgtgatgaaa gtgcagatga
     3241 ggtcagacga tgcaggtcgg tgagtccgtg gtgaacgcga tagtggaata ccacgcacag
     3301 ggcagtgagg aggcgcatgc agaggaaaac aggatgttcc tgcgttatct gcacgagtta
     3361 acgggaacac agcgtgtggc ggttgaggtg gccaggaaat atttttcgcc ggtgagggct
     3421 gatatgagct acctttttct tgtcgccggc accgcaccag gtgcgacggt ccttttacag
     3481 aattgtcctc atttgacggg cctggatggc ccgttctttt atgcttttaa cgtccggggc
     3541 ccttttctcg gccgaaatca ccggctatgc ggtggtgtgc atagccatgc atatgcgtaa
     3601 aataaatcag gcgcgtttct ggcggttttc ggtgtacttg ccaccacctt ttgcccgtta
     3661 accaccagaa acgccctgag gccgttttcg cggtgcgcgt aagaagacgt tatggtaaat
     3721 agggcgctat cgcctaaata cacggctgat tgtttttttt tctttgtagt atgagattcc
     3781 tgagtagaca gctacaacaa gtagagcaat ggcgatagca agtgaaatcc agtccataaa
     3841 tgcctgatta atttaataaa aacagcctgt tatgatttca taaagataat atcatctttt
     3901 tttagcatga tactgtcgtt acttattgtg gtgagatgaa acataaccgc tgtttttgta
     3961 tgtgtttgtt aaggctgcta agattattct taaccactgg ttttgtacat atcatcaaaa
     4021 aaaaaataaa aaaaactaat gcccccgtgg tgggtatata acaaatcacg gggagcgggt
     4081 cagaactacg ccaaaacttt gaatgatcgg ggggcagact aaatgaaatc ttgagattca
     4141 ttcagcctcg tcgtaatctc ttgctctgta aacgaaaaaa ccgcccagca aggcggtttt
     4201 ctcgaaggtt ctcagagcag caactctttg aaccgaggta actggctagt tagaggagcg
     4261 cagtcaccaa aactgttcgt ttagtttagc cttaaccggc gcataagttc aagactatct
     4321 cctctaaatc agttaccagt ggctgctgcc agtggcgctt ttgcatgtct ttccgggttg
     4381 gactcaagac gatagttacc ggataaggcg cagcagtcgg actgaacggg gggttcgtgc
     4441 atacagtcca gcttggagcg aactgcctac ccggaactga gtgtcaggcg tggaatgaga
     4501 taaacgcggc cataacagcg gaatgacacc ggtaaaccga aaggcaggaa caggagagcg
     4561 cacgagggag ccaccagggg gaaacgcctg g
//
