LOCUS       NZ_CP034043             3511 bp    DNA     circular CON 26-NOV-2023
DEFINITION  Klebsiella pneumoniae subsp. pneumoniae strain CRK0298 plasmid p4,
            complete sequence.
ACCESSION   NZ_CP034043
VERSION     NZ_CP034043.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN08579613
            Assembly: GCF_002970375.3
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae subsp. pneumoniae
  ORGANISM  Klebsiella pneumoniae subsp. pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3511)
  AUTHORS   Fouts,D., White,R., nguyen,K., Singh,I. and D'Souza,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2018) Informatics Core Services, JCVI, 9605
            Medical Center Drive, Rockville, MD 20850, USA
REFERENCE   2  (bases 1 to 3511)
  AUTHORS   Vanduin,D., Fouts,D., Sutton,G., Nguyen,K., Hujer,A., Hujer,K. and
            Bonomo,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-OCT-2018) Informatics Core Services, JCVI, 9605
            Medical Center Drive, Rockville, MD 20850, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: OCT-2018
            Assembly Method                   :: Unicycler v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 121.2238x
            Sequencing Technology :: Illumina NextSeq; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/26/2023 02:31:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,746
            CDSs (total)                      :: 5,628
            Genes (coding)                    :: 5,518
            CDSs (with protein)               :: 5,518
            Genes (RNA)                       :: 118
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 110
            CDSs (without protein)            :: 110
            Pseudo Genes (ambiguous residues) :: 0 of 110
            Pseudo Genes (frameshifted)       :: 43 of 110
            Pseudo Genes (incomplete)         :: 61 of 110
            Pseudo Genes (internal stop)      :: 19 of 110
            Pseudo Genes (multiple problems)  :: 11 of 110
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP034043.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3511
                     /organism="Klebsiella pneumoniae subsp. pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="CRK0298"
                     /isolation_source="miscellaneous body fluid"
                     /host="Homo sapiens"
                     /sub_species="pneumoniae"
                     /db_xref="taxon:72407"
                     /plasmid="p4"
                     /geo_loc_name="USA: Ohio"
                     /collection_date="2015"
     gene            join(3181..3511,1..11)
                     /locus_tag="C5X33_RS29245"
                     /old_locus_tag="C5X33_29245"
     CDS             join(3181..3511,1..11)
                     /locus_tag="C5X33_RS29245"
                     /old_locus_tag="C5X33_29245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_071599364.1"
                     /translation="MADKRNKMLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPTR
                     AGRLPSISPALLRQLAGMGNNLNQIARKVNAGGAGHDRVQIVAALMAIDAGLERLRHAV
                     LEKGPDDDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            220..>648
                     /locus_tag="C5X33_RS30905"
                     /pseudo=""
     CDS             220..>648
                     /locus_tag="C5X33_RS30905"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            689..1186
                     /locus_tag="C5X33_RS29255"
                     /old_locus_tag="C5X33_29255"
     CDS             689..1186
                     /locus_tag="C5X33_RS29255"
                     /old_locus_tag="C5X33_29255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_015328238.1"
                     /translation="MSSLLTASRDFEEKSKAQQQLTEERLKAAFSKHENVVKSELNASA
                     KRINDAISAHEQGMNAAMQSNRLSVLRMVGRTWLTITMVSGLLFASLSGVLWYQGNRIA
                     ANLAEIRQQRDTLSKLHMQTWGVTYLENRNGRFLVLPEGMKAETGRTVDNKTRNAVKLV
                     RE"
                     /name="MbeB family mobilization protein"
     gene            1193..1408
                     /locus_tag="C5X33_RS29260"
                     /old_locus_tag="C5X33_29260"
     CDS             1193..1408
                     /locus_tag="C5X33_RS29260"
                     /old_locus_tag="C5X33_29260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015328239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_015328239.1"
                     /translation="MTELEKQLLSALEQLQQDYSRRLDEWESAFEEWRKMSGLMQRENA
                     ALSERVSGLSRQVQSLSEQLRRLSTG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            1803..1985
                     /locus_tag="C5X33_RS29265"
                     /old_locus_tag="C5X33_29265"
     CDS             1803..1985
                     /locus_tag="C5X33_RS29265"
                     /old_locus_tag="C5X33_29265"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016265984.1"
                     /translation="MLTFIGVVVVAVIAFKLFMLFFSSSESAVERATRKYMENPTSANY
                     KIMVATRMRNEKKRG"
                     /name="hypothetical protein"
     Typing          2923..3096
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.5600000000000002e-86"
     Typing          complement(2435..2956)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.69"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          2..1524
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3203..3510
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.308"
                     /coverage="97.0"
                     /evalue="2.83e-57"
ORIGIN
        1 atgattgtta aattccatcc ccggggtcgc ggcgggggcg ccggtcctgt ggattatctt
       61 ctgggtaaag accggcagcg tgaaggtgcc agcgtcctgc aggggaagcc ggaagaagtc
      121 cgggagctga ttgatgcgtc gccgtatgtg aaaaaatata cctccggggt gctgtcattc
      181 gcggaggcgg acctgccgcc gggtcagcgt gaaaaactga tggccagctt tgagcgggtt
      241 ctgatgcccg gactcgataa agaccagtac agcattctgt gggtggaaca cgcagacaag
      301 ggtcgtcttg agctgaattt tctgatcccc aataccgaat tactgacggg gaaacgcctc
      361 cagccgtatt acgatcgtgc cgaccggccg cgcatcgatg cgtggcagac agtggtcaac
      421 ggcaggctgg ggctacatga tccgaacgac ccggagaacc ggcgggcgct ggtcacgcct
      481 tcaggactgc cagaaacgaa gcaggaagcc gcagagacga ttacacgggg tttgctgatg
      541 ttagcctcat caggagagct gaaaacgcgt gatgacgtca caggggcgct cacagcggcc
      601 ggttttgagg tggtcagaac cacaaaaaac agtatcagca ttgccgaccc ggacgggggg
      661 cgaaatatcc gactgagggg agccatttat gagcagtctt ttaacggcct cgagggactt
      721 cgaggagaaa tcgaaagcac agcagcagct taccgaagag aggctgaaag ccgcattcag
      781 caagcacgag aacgttgtca aatcggaact gaacgcaagc gcgaagagaa tcaacgacgc
      841 catcagcgcc cacgaacagg ggatgaacgc ggcgatgcag tcgaaccggc tgagcgtgct
      901 gcgaatggtc gggcggacgt ggctgaccat caccatggtg tcagggctgc tgttcgccag
      961 cctgagcggg gtgctgtggt accaggggaa ccggatagcg gcgaacctgg cggaaatcag
     1021 gcagcagaga gatacgctgt cgaaactgca catgcagacg tggggcgtga cctatctgga
     1081 gaaccgcaac ggacggtttc tggtgctgcc ggaggggatg aaagccgaaa cgggccggac
     1141 ggtggacaac aagacgcgga acgcggtgaa gctggtacgg gagtagagca gaatgacgga
     1201 actggaaaaa caattgctga gcgcgttaga gcagctacag caggactact cgagaaggct
     1261 ggacgagtgg gagagcgcct tcgaggaatg gcggaagatg tctggtctta tgcaacggga
     1321 gaacgcggcg ctgagcgagc gcgtgtcggg cttgagcagg caggtgcaga gtttgagcga
     1381 gcagctgcgc cggttatcga caggctgaat gagattgagc aacagcgata cgggcaaaca
     1441 acggaaaagg aaccagagaa gacagcagat ctggtaccgg aacggcaacg cacattatct
     1501 catggtgaca gggagtatgg agactgataa acgcccgtct caggtggcct gagcgtgacg
     1561 gggatcatgg ccattttctg cgatttcagc acacagagag tcttttttaa ccgaaatcac
     1621 cggctatgca gtgatgtgaa taagtgatgc atatgcgtaa aataaatcag gtgcgtttct
     1681 ggtggttttc ggtgtacttg ccaccacctt ttgcccgtca gccatcagaa acgccctgag
     1741 tgcctccggc gcggtgcggg taagagatgt tatgtttaat gttgataaca ggagtaagag
     1801 tgatgcttac atttattggt gtagtggtag tcgccgttat tgcatttaaa ttatttatgc
     1861 ttttttttag ttcatccgaa tctgccgtcg aacgtgctac ccgtaaatat atggagaacc
     1921 ctacgagtgc taactataaa ataatggtgg ctacacgcat gagaaatgag aagaaaagag
     1981 ggtgatatat gtttgagttt attggttttt tatttttact tttggtgtgt tatgtcttta
     2041 tacaaatggt tgctgtcagg gtgtttcctg aatatggagt acggaaagcg gaggaacgat
     2101 accgtcgaaa tccatcagca gaaaatgagg atctgttatg gagagctaga agtcgtataa
     2161 ctaaacgcaa gtaatgtttc ctaagtgtgt tgataggcaa acatggtggg agttactact
     2221 ctcggagtta atgatcttct tgtgatcctg tcgtttcagt cgtaatctct tgctctgaaa
     2281 acgaaaaaac cacctgggga ggtggtttga tcgaaggtta agtcagttgg ggaactgctt
     2341 aaccaggtaa ctgggtatac gagaccgcag ataccaaaac tgttctttca gtgtagccgc
     2401 agtcaggccc atacttcaag acctctgaat ctctctctcg tatatcccag ttaccagtgg
     2461 ctgctgccag tggcgctttg tcgtgctttc tcgggttgga ctcaagatga tagttaccgg
     2521 gtaaggcgca gcagtcggac tgaacggggg gtttgtgcat acagtccagc ttggagcgaa
     2581 ctgcctaccc ggaactgagt gtcaggcgtg gaatgagata aacgcggcca taacagcgga
     2641 atgacaccgg taaaccgaaa ggcaggaaca ggagagcgca cgagggagcc gccaggggga
     2701 aacgcctggt atctttatag tcctgtcggg tttcgccacc actgatttga gcgtcagatt
     2761 tcgtgatgct tgtcaggggg gcggagccta tggaaaaacg gcttcgccgc ggccctatcg
     2821 cttccctgtt aagtatcttc ctggcatctt ccaggaaatc tccgccccgt tcgtaagcca
     2881 tttccgctcg ccgcagtcga acgaccgagc gtagcgagtc agtgagcgag gaagcggaat
     2941 atatcctgta tcacatattc tgctgacgcg ccggtgccgc cttttttctc ctgccacatg
     3001 aagcacttca ctgacatcct catccgtgcc aacatagtaa gccagtatac actccgctag
     3061 cgctacgtga ctggttcagg gctgcgcccc gaaaccccac tgacgcgcct gcggcttgtc
     3121 caaccccgcg ccgaccggga aaggctttcc cgtccgtcac ggcgttatca ggaggcgatt
     3181 ctggcagata aacgcaacaa gatgctcacg atgtgggtga cggaagacga acaccggcgg
     3241 ttgcttgagc ggtgcgacgg aaaacaactg gcggcgtgga tgcgccagac ctgccttgat
     3301 gagaaaccga cacgcgccgg caggcttccc tcgatttcgc ccgcgctgct gcgtcagctt
     3361 gccggcatgg gcaataacct gaaccagatc gcccggaaag tgaacgccgg tggtgccggt
     3421 catgaccggg tgcagatcgt ggcagcgctg atggcgatcg atgccggtct ggaacggttg
     3481 cggcatgccg tactggaaaa agggccggac g
//
