LOCUS       NZ_CP033965             5596 bp    DNA     circular CON 08-JAN-2024
DEFINITION  Klebsiella pneumoniae strain L482 plasmid p6_L382, complete
            sequence.
ACCESSION   NZ_CP033965
VERSION     NZ_CP033965.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN10434199
            Assembly: GCF_006494915.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Yu,X., Zheng,B. and Xiao,Y.
  TITLE     Uncovering the gut microbiota of hospitalized patients served as the
            reservoirs of ST11 KPC-2-producing Klebsiella pneumoniae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Yu,X., Zheng,B. and Xiao,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-NOV-2018) State Key Laboratory for Diagnosis and
            Treatment of Infectious Diseases, The First Affiliated Hospital,
            Zhejiang University, Qingchun, Hangzhou 310003, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: AUG-2017
            Assembly Method                   :: HGAP v. 2.3
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 98.0x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/08/2024 05:14:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,927
            CDSs (total)                      :: 5,803
            Genes (coding)                    :: 5,645
            CDSs (with protein)               :: 5,645
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 62 of 158
            Pseudo Genes (incomplete)         :: 102 of 158
            Pseudo Genes (internal stop)      :: 21 of 158
            Pseudo Genes (multiple problems)  :: 27 of 158
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP033965.1.
            Bacteria and source DNA available from Xiao Yonghong.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="L482"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p6_L382"
                     /geo_loc_name="China:Zhejiang"
                     /collection_date="2017-08"
                     /collected_by="xuhao"
     gene            join(5386..5596,1..299)
                     /locus_tag="EHC63_RS30040"
     CDS             join(5386..5596,1..299)
                     /locus_tag="EHC63_RS30040"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            306..518
                     /locus_tag="EHC63_RS30045"
     CDS             306..518
                     /locus_tag="EHC63_RS30045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            1185..1409
                     /locus_tag="EHC63_RS30050"
                     /old_locus_tag="EHC63_30420"
     CDS             1185..1409
                     /locus_tag="EHC63_RS30050"
                     /old_locus_tag="EHC63_30420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            1393..1659
                     /locus_tag="EHC63_RS30055"
                     /old_locus_tag="EHC63_30425"
     CDS             1393..1659
                     /locus_tag="EHC63_RS30055"
                     /old_locus_tag="EHC63_30425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(1729..2079)
                     /locus_tag="EHC63_RS30060"
                     /old_locus_tag="EHC63_30430"
     CDS             complement(1729..2079)
                     /locus_tag="EHC63_RS30060"
                     /old_locus_tag="EHC63_30430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(2145..2690)
                     /locus_tag="EHC63_RS30065"
                     /old_locus_tag="EHC63_30435"
     CDS             complement(2145..2690)
                     /locus_tag="EHC63_RS30065"
                     /old_locus_tag="EHC63_30435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            complement(2716..3042)
                     /locus_tag="EHC63_RS30070"
                     /old_locus_tag="EHC63_30440"
     CDS             complement(2716..3042)
                     /locus_tag="EHC63_RS30070"
                     /old_locus_tag="EHC63_30440"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            complement(3400..3558)
                     /locus_tag="EHC63_RS31325"
     CDS             complement(3400..3558)
                     /locus_tag="EHC63_RS31325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            4198..4374
                     /locus_tag="EHC63_RS30745"
     CDS             4198..4374
                     /locus_tag="EHC63_RS30745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            4364..4708
                     /locus_tag="EHC63_RS30080"
                     /old_locus_tag="EHC63_30450"
     CDS             4364..4708
                     /locus_tag="EHC63_RS30080"
                     /old_locus_tag="EHC63_30450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(4890..5135)
                     /locus_tag="EHC63_RS31130"
     CDS             complement(4890..5135)
                     /locus_tag="EHC63_RS31130"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032433971.1"
                     /translation="MQPQSPVHHGLPRIDARTVCPVVVRLETPAGQQFRVRYQKIQFQP
                     PLVPVFNPQHAVLVFIESGHQNPLEARHQLFTLAGR"
                     /name="hypothetical protein"
     Typing          4033..4112
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.361"
                     /coverage="97.0"
                     /evalue="1.1100000000000001e-21"
     Typing          complement(3450..3847)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          3..634
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.526"
                     /coverage="99.0"
                     /evalue="1.5100000000000001e-109"
     Typing          4699..5594
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 tgcgtatggt cgggcggaca tggctgacca tcacgatggt gtcaggactg ctgttcgcca
       61 gcctgagcgg ggtgttgtgg tatcagggga gcttgatagc gtcaaatctg gccgaaatcg
      121 accggcagaa cgcagcgctg tcgaagctga acgcgaagac ctggggcgtg acgtacctgg
      181 aagacagcaa cggacgtttt ctggtgctgc cgaaggggac ggccgtagac aggacgcaga
      241 gctggacggt cgggaacggg cggtcgaagc agaacgcgct gagactggtg aaggagtaag
      301 tgccgatgac cgagctggaa aaacagttgc tgagcgcatt agagcagcta cagcaggact
      361 actcgaaaag gctggacgag tgggagagcg ccttcgggga atggcggaaa atgtcagggc
      421 ttatgcaacg ggagaacgcg gcgctgagcg agcgcgtgac gcgcttgagt cagcaggtgg
      481 aacgcttgag cgggcaactg cagcgtttga gccggtagtg cagcgtcatg agatggccgt
      541 ggccgcagaa cgggcgcacg agcaacggca gcacgaaaag gaactgacgg aagcgcgttc
      601 ccggcagcga agctatgacg gtccgtcgct gggctgaaat gcaggaaaac agggttctca
      661 tcgggcattt acgctgaaac cggatgtcat tcacctggct gcatggctat gcagccaggt
      721 aaaatttatc tgtcgcgaac ggggcggttt atcgggtggt ttgttgccgg ttttacctga
      781 ctgccacccc gttcgcggcg aacccgtccg gggcggtgcg ggcaacgggt gttatgttta
      841 atcagcgtga acggcagata caaaaaagcc ccggagggct tttaactact gacttatttt
      901 tttgaagatt tgctgggttt caatttgtca gtatttcccg gcgttttttt attgtcacgc
      961 tggcgtttat caggttcccc tgttgaatcg gcaattcgtt tcggacccgg cttaagaccc
     1021 ataatcgtta tcctttcaat agttaagagg aaagccctca agctagatat attgaccgcg
     1081 ttacggatta tcaagagggg ctaacaagat tattttttgt tcttttaggt tgattgtggt
     1141 actgttgata tacatgtata gcaaatggtg ttcgggaggt ttatatgctt gctatccggt
     1201 tatctgatga gattgagtcc cgtctggact cgctggcgaa gcaaaccggc agaacaaaga
     1261 cgttttatgc gcgggaagca atactggcgc atctggaaga cctggaggat tattatcttt
     1321 cagcagaaac tgctgcacgc gttcgccgtg gtgatgaagc agtgcattcg tctgaagacg
     1381 tgaggaagtc acttggtctg gacgattaac tattccgatc gggcgctcaa atcgttacgc
     1441 aagatggaca aacagaacgc acgacggatt gtggatttta tgagtttacg cattgcagtt
     1501 gctgccgatc ctcgccagtc agggaagccg ctcaaaggtg agctgggcga gttctggcgc
     1561 tatcgggtgg gagattatcg cgttctgtgt gagatccgag atgacgagct tgttatcctt
     1621 gccgccacga ttggacatcg ccgcgaagtt tacgactgaa gcccgctatg cgggcttttt
     1681 tgatgccgac aacaggtatt atggtaaata ctcgactaac tacgtgttct actgtttgtc
     1741 ggttttagtt aaccagcagg ttgtcttaac gataatgtag ccgttatttt taactaataa
     1801 ggtcatattg ttaacgtcag gcataataaa agacattatg gcctcagagt tatcatcaga
     1861 agcagctctc tgcattacta cttcgattcc cttttctttg aatctataag tatccagttg
     1921 cctattaaac ttagaaagac ctacaaccat acttagtttg ttatctttaa cagtaagaga
     1981 tgcttcctga gtaccattac atcggtagta agcggtgtaa ccgtcagcaa aagatcgacc
     2041 ggggaatata aatgctagga acgcaattaa ccaatacata gttgagaatc ttggtattac
     2101 acatttcact atcctctctt tcatttttcg ttcaccatac tttgttatct gatttttaat
     2161 ttttctattt tataaaccaa gtctctacca aaaaggcgaa aattatcctc aaataataat
     2221 aattgtttaa gaacatccat gaaagcagct gcttcaccct cagttatggg agctactcta
     2281 tataacatat aacctgagtt aagggtttct tttatacttt cttcaatgct cattataaat
     2341 attagtggat tatcattttt cttcccccat gggtttctaa ttaatccttc gatgtactca
     2401 ggtcctttgc gtcctattgc ctgcgcaata ttttttattt catctgcgtg agggctttgt
     2461 tgtacagctt tgtttatgcc tgcttgagtg attttcagag actctacacc gtgtatgttg
     2521 tatgagataa aatgtaataa attaagatgc aatgcaatta atccattttt gtttacatct
     2581 gagtatttgg ataaaaaacg taatgaagtg ttaactctcc cttgaagcct aacaattggg
     2641 agtgctgcct tgtatatttc cttctccgtt aaataccgtt taaaaaacat aattctccgc
     2701 ctccgtatag cgtgcctatt ttatttcgtc gtaaaactta gctctgaaat attcctttag
     2761 aaccatcatg tcattaataa ttgaatctat ctcatccatt ttttcttggt attcttttaa
     2821 tggtgaaggg agcggtaact caatatttga aaagtgattg gcgagatagg gatcaaggtt
     2881 tctcatccat tcaggctttt cgccaatgcg ttcttcaact gaaagtgaac ctgataatct
     2941 ctggttaata agatcactta gactcatttt ggttttattc gaaagccgtt gtaaggattg
     3001 ctttaatcct atatctagca tgatatttgt tctttctctc attacccccc cttgctattg
     3061 cgttttttgt tgcgcacaaa gctaccattg cgcattaaga aacgcaataa aaaaggaggg
     3121 agggagtggt ttgtacatga tgtgcagtgc gctgagggca tcagtggcgg gtgtttttcc
     3181 gtgggatgga tggagctccg gggataacag aactgacgat catttaaagg atcttcttga
     3241 gatccttttt ttctgcgcgt aatctcttgc actgtaaacg aaaaaaccgc ctggggaggc
     3301 ggtttgatcg aaggttaagt cagttgggga actgcttaac cgggtaactg ggtatacgag
     3361 accgcagata ccaaaactgt tctttcagtg tagccgcagt taggcaccca cttcaagacc
     3421 tctcaatatc tctctcgtat atatccagtt accaatggct gctgccagtg gcgtttttgc
     3481 gtgtccgtcc gggttggact caagatgata gttaccggaa agggcgcagc agtcgggctg
     3541 aacggggggt tcgtgcatac agcccagctt ggagcgaacg acctaaaccg agccgagata
     3601 ccagcagcgt gagctatgag aaagcgccac gcttcccgaa agggaaaaag gcggaacagg
     3661 tatccggtaa acggcagggt cggaacagga gagcgcacga gggagccgcc ggagagaaat
     3721 caccggcatc tttaagtcct gtcgggtttc gtcctcctct gacttgagcg tccatttctg
     3781 tgatgctcgt caggggaggc ggagcctatg gaaaaacggc aacgccgcat ccttttctcc
     3841 ggccctttct gatttgagcg cctttgcttt atcccgttat ccgtgagtcg gtcacgcccg
     3901 ccgcagccga acggcaggag cgcagctacc gagtgagcga ggaggcgtca tttcatctgt
     3961 ccgtgcatgg tattgcggac gctcctgagc cacatatcac acctgccaca tgaggcactt
     4021 tcatgaatcg tgctctcatg ccaacatagt aagccagtat acacagcgta aacgctgtga
     4081 ctggttcagg gctgcgcccc gaaacccgct aacaccactg acgcgctggc gcttgtccaa
     4141 cgccggagcg acccggaaaa cttttccgtg ccgtccggcg tttgcgaagg agggataatg
     4201 cagaagccga agaagaagcc gctcgtcgta aaaaaaagat cctggcatcc ggcagtgatg
     4261 gcaaatctga tgcgtcaggc acaggaggaa gattaccgcc gggagcagat ggcagagctt
     4321 gagcgttcag cccggtcaca ttttgcggat atcggaggag aggatggcgg ttaagcgtac
     4381 ccgtttttta ggtatccggg tgaccgatgg ggagtaccag cagctgctgg agcgctgcaa
     4441 tggaaggcaa ctggcggtgt ggatgcggga aacctgtctc gatacgcgcc cggcgcggtc
     4501 gttgcggctg ccgtccatcg atccggtttt gctgcgccag cttgccggga tggggaacaa
     4561 cctcaaccag atcgcccgga aaattaacgg cggccagtgg tcaggcgctg accgggttca
     4621 ggtggtggcc gcgctgatgg ccattgatgc cgaactcgag cggctgcggc atgccgtgct
     4681 ggaaaagggg gcagacgatg atcgttaagt ttcatccgcg agggcgaggc ggcggtgccg
     4741 ggccggtgga ttatctgctg gggaaagacc gccagcgcga cggggccatc gtcctgcagg
     4801 gaaagccgga ggaggtccgg gagcttatcg acgcctcgcc ctacgccaaa aagtacacct
     4861 ccggcgtcct gtcttttgct gaacaggatt taccgcccgg ccagcgtgaa aagctgatgg
     4921 cgagcttcga gcgggttctg atgcccggac tcgataaaga ccagtacagc gtgctgtggg
     4981 ttgaacaccg ggacaagggg cggctggaac tgaattttct ggtaccgaac acggaactgc
     5041 tgaccggcag gcgtctccag ccgtactacg accgggcaga ccgtccgcgc atcgatgcgt
     5101 ggcagaccgt ggtgaacggg cgactggggc tgcacgaccc gaacgcaccg gagaaccggc
     5161 gcgcgctggt gacgccatct tcgttaccca aaacgaagca ggaagccgca gaggctatta
     5221 cgcggggctt actggccctc gcctcgtcag gggagcttaa aacgcgtcag gacgtcactg
     5281 aggcgctgga aagcgcaggt tttgaggtcg tgcgcaccac aaagagcagc atcagcattg
     5341 ccgacccgga cggagggcga aacatccgac ttaagggagc catctatgaa cagtctttta
     5401 acgctggcga aggacttaga gcagaaatcg aaagcgcagc agcagagtac cggcgaaatg
     5461 ctgaaagccg cattcagcga gcacgagaag tctgtcagag cggaactgag cgaaagcggg
     5521 aagagaatca gcgccgccat ccgcgcccac gagcagggta tgagcgcggc catgcagtcg
     5581 aaccgcctga gcgtga
//
