LOCUS       NZ_CP033964            11970 bp    DNA     circular CON 08-JAN-2024
DEFINITION  Klebsiella pneumoniae strain L482 plasmid p5_L382, complete
            sequence.
ACCESSION   NZ_CP033964
VERSION     NZ_CP033964.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN10434199
            Assembly: GCF_006494915.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 11970)
  AUTHORS   Yu,X., Zheng,B. and Xiao,Y.
  TITLE     Uncovering the gut microbiota of hospitalized patients served as the
            reservoirs of ST11 KPC-2-producing Klebsiella pneumoniae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11970)
  AUTHORS   Yu,X., Zheng,B. and Xiao,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-NOV-2018) State Key Laboratory for Diagnosis and
            Treatment of Infectious Diseases, The First Affiliated Hospital,
            Zhejiang University, Qingchun, Hangzhou 310003, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: AUG-2017
            Assembly Method                   :: HGAP v. 2.3
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 98.0x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/08/2024 05:14:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,927
            CDSs (total)                      :: 5,803
            Genes (coding)                    :: 5,645
            CDSs (with protein)               :: 5,645
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 158
            CDSs (without protein)            :: 158
            Pseudo Genes (ambiguous residues) :: 0 of 158
            Pseudo Genes (frameshifted)       :: 62 of 158
            Pseudo Genes (incomplete)         :: 102 of 158
            Pseudo Genes (internal stop)      :: 21 of 158
            Pseudo Genes (multiple problems)  :: 27 of 158
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP033964.1.
            Bacteria and source DNA available from Xiao Yonghong.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11970
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="L482"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p5_L382"
                     /geo_loc_name="China:Zhejiang"
                     /collection_date="2017-08"
                     /collected_by="xuhao"
     gene            join(10566..11970,1..281)
                     /locus_tag="EHC63_RS29960"
                     /old_locus_tag="EHC63_30335"
     CDS             join(10566..11970,1..281)
                     /locus_tag="EHC63_RS29960"
                     /old_locus_tag="EHC63_30335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            291..548
                     /locus_tag="EHC63_RS29965"
                     /old_locus_tag="EHC63_30340"
     CDS             291..548
                     /locus_tag="EHC63_RS29965"
                     /old_locus_tag="EHC63_30340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            633..782
                     /locus_tag="EHC63_RS29970"
                     /old_locus_tag="EHC63_30345"
     CDS             633..782
                     /locus_tag="EHC63_RS29970"
                     /old_locus_tag="EHC63_30345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            2266..4230
                     /locus_tag="EHC63_RS29980"
                     /old_locus_tag="EHC63_30355"
     CDS             2266..4230
                     /locus_tag="EHC63_RS29980"
                     /old_locus_tag="EHC63_30355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            4230..4961
                     /gene="mobC"
                     /locus_tag="EHC63_RS29985"
                     /old_locus_tag="EHC63_30360"
     CDS             4230..4961
                     /gene="mobC"
                     /locus_tag="EHC63_RS29985"
                     /old_locus_tag="EHC63_30360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            4968..5498
                     /locus_tag="EHC63_RS29990"
                     /old_locus_tag="EHC63_30365"
     CDS             4968..5498
                     /locus_tag="EHC63_RS29990"
                     /old_locus_tag="EHC63_30365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(5525..5704)
                     /locus_tag="EHC63_RS29995"
                     /old_locus_tag="EHC63_30370"
     CDS             complement(5525..5704)
                     /locus_tag="EHC63_RS29995"
                     /old_locus_tag="EHC63_30370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(5730..6158)
                     /locus_tag="EHC63_RS30000"
                     /old_locus_tag="EHC63_30375"
     CDS             complement(5730..6158)
                     /locus_tag="EHC63_RS30000"
                     /old_locus_tag="EHC63_30375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(6322..6636)
                     /locus_tag="EHC63_RS30005"
                     /old_locus_tag="EHC63_30380"
     CDS             complement(6322..6636)
                     /locus_tag="EHC63_RS30005"
                     /old_locus_tag="EHC63_30380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(6624..6902)
                     /locus_tag="EHC63_RS30010"
                     /old_locus_tag="EHC63_30385"
     CDS             complement(6624..6902)
                     /locus_tag="EHC63_RS30010"
                     /old_locus_tag="EHC63_30385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(7077..7442)
                     /locus_tag="EHC63_RS31125"
                     /old_locus_tag="EHC63_30390"
     CDS             complement(7077..7442)
                     /locus_tag="EHC63_RS31125"
                     /old_locus_tag="EHC63_30390"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            complement(7439..7810)
                     /gene="tolA"
                     /locus_tag="EHC63_RS30020"
                     /old_locus_tag="EHC63_30395"
     CDS             complement(7439..7810)
                     /gene="tolA"
                     /locus_tag="EHC63_RS30020"
                     /old_locus_tag="EHC63_30395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(8084..8329)
                     /locus_tag="EHC63_RS30025"
                     /old_locus_tag="EHC63_30400"
     CDS             complement(8084..8329)
                     /locus_tag="EHC63_RS30025"
                     /old_locus_tag="EHC63_30400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            8974..10461
                     /gene="ltrA"
                     /locus_tag="EHC63_RS30035"
                     /old_locus_tag="EHC63_30410"
     CDS             8974..10461
                     /gene="ltrA"
                     /locus_tag="EHC63_RS30035"
                     /old_locus_tag="EHC63_30410"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226014.1"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="WP_004178082.1"
                     /translation="MMYGEEKSDSLIVAAKQANNPKGAESVERRSGAKGNAEQPHMRRT
                     QSRESMSQRLSRVREAAKQRKKERFTALFHLLTVEALEAAFLSLSRKAAAGVDGIRWMD
                     YAGNMKNNITDLHRRLHQGSYRAQPGRRHYIPKADGKQRPLGIASLEDKIVQYALVKIL
                     NAVYENDFMGFSYGFRPGRSQHDALDALATGLVRTNVNWVLDADISQFFDRVSHEWLIR
                     FTEHRIGDRRVIRLIRKWLTAGTSEEGQWRATEEGTPQGAVISPLLANIYLHYVFDLWA
                     HQWRRRYATGNVVMVRYADDIVIGFDKRYDARRFRIAMQRRLREFGLTVHPEKTRLMEF
                     GRFAAENRAIRGKGKPETFNFLGFTHISGKDRNGRFMLIRKTRRDRMTATLKAIKDGLR
                     RRWHYSIPEQGKWLRRVVQGYLNYHSVPGNFPTMQKFRTHVTNLWRRALRRRSQKDDTT
                     WTKANKLAAAWLPRVRVLHPWPVERFTARHPRQEPGA"
                     /name="ltrA"
     BGC             5566..11970
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(886..1464)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          4231..4958
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.35e-140"
ORIGIN
        1 actacggcca tgattatcat ccagtaccta aaacagaaga cataaaggga cttggagagt
       61 taaagcgagg tgatcctaaa acacctaaac aaggtggtgg tggtaagcgg gcccgatggt
      121 atggcgataa aaaacgtaaa atttatgaat gggattccca gcacggtgag cttgaagggt
      181 atcgtgccag tgatggcgaa cacctaggcg catttgatcc caaaacgggt aagcaggtta
      241 aagggccaga tccaaaacgg aacattaaaa aatatctttg agaggtaaat atggggctta
      301 aattacgttt gacatggttt gataagaaaa ctgaagagtt taaaggggaa gagtattcta
      361 aagactttgg tgacgatggt tctgtcattg aaagcttggg aatgccttta aaggataata
      421 ttaataatgg tgagtttgat ttgaagaaag aatgggttcc tttactacag ccatttttta
      481 aaaatcaaat aaaacctgat gtggataatt gttatatagc ttttgattat cgggacggta
      541 actggtaaca aaaaattatc aaggatgatt tgaaatactg acttccggtg ttcagggatg
      601 aatgccggag tctttaaata cagggaaagt atatgaaaaa ggtaaagact atttttcttt
      661 ttatattgat tgttgctggc tttcttctgg ccgcatgtca ggcaaattat atccgggatg
      721 ttcagggcgg aacggtggca ccatcgtcct cctctgaact gacggggatc gcggttcagt
      781 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct tttgccctgt
      841 aaacgaaaaa accacctggg gaggtggttt gatcgaaggt taagtcagtt ggggaactgc
      901 ttaaccgtgg taactggctt tcgcagagca cagcaaccaa atctgtcctt ccagtgtagc
      961 cggactttgg cgcacacttc aagagcaacc gcgtgtttag ctaaacaaat cctctgcgaa
     1021 ctcccagtta ccaatggctg ctgccagtgg cgttttgcgt gctttcccgg gttggactca
     1081 agtgaacagt taccggataa ggcgcagcag tcgggctgaa cggggagttc ttgcttacag
     1141 cccagcttgg agcgaacgac ctacaccgag ccgagatacc agtgtgtgag ctatgagaaa
     1201 gcgccacact tcccgcaagg gagaacggcg gacaggtatc cggtaaacgg cagggtcgga
     1261 acaggagagc gcaagaggga gcgacctacc ggaaacggtg gggatcttta agtcctgtcg
     1321 ggtttcgccc gtactgtcag attcatggtt gagcctcacg gctcccacag atgcaccgaa
     1381 aaagcgtctg tttatgtgaa ctctggcagg agggcggagc ctatggaaaa acgccaccgg
     1441 cgcggccctg ccgttttgcc tcacatgtta gtcccctgct tatccacgga atctgtgggt
     1501 aactttgtat gtgtccgcag cgcccgccgc agtctcacgc ccggagcgta gcgaccgagt
     1561 gagcgaggag gcgcaatttc cccggcacct gcaagacggt caccggacgc gctgatgtgg
     1621 gatattgtcc cctgattaag tctttctatt ccctgacgat caattatcag gataggtgaa
     1681 ccatgtcggt ttttcgctga acattttacg ttaacctatc tctgttgctt ttttctcctt
     1741 gcggagaaat gcaagtcatc cccgctgggg cgtggctgac ttcaggggaa aagaccttgc
     1801 ttacgctggc tggacgccac cgacgcacgg tcttttcccc tctttctggc tctgtccggc
     1861 tggacgccgg ccacgccaga aattcacccg gcccccgtca ggctcacgcc tgaccaccca
     1921 caaaaatcca cggtgctgga cgcacctgac gaagcagcaa agctgctcct gattttgcgg
     1981 gtggtcagcc gctcgctatg gcgaactctg acgggggccg gaacaccgga aaagaccgca
     2041 ctggggtgcc gccttttcct ctcacacgct ggggcgtgct cgttgttcct ccggggctgc
     2101 gccttttccg catggggcgg aaagaggctg gcctccgcgg tgaaagctga acgcaatcac
     2161 cacggacgtg accgccgccg ccatgccttg gggcatgtcc gcggcttgtg cagcgggggc
     2221 tgcgcatcac gccggtaaac ccgtcgtctg aaggagagag attgcatgtt taatacggat
     2281 tcgcttgcct ggcagtggca tatcccggag ctggcccggc agatttaccc gtgggtcagc
     2341 tggctgtacc gggagtatta cagtctggcc ggtttttttc tggttatcgg cctgtttttc
     2401 ggtctggctg aactctgggc cggccgaatg cgttttgtgg ggctgagcca taaggcgcgg
     2461 cgggagtatg ccgatcgcta taagcgcgat aataaccgga ctgtgccatt ttcggtgctg
     2521 gcctcgctgg attcacccgg gcggatggtc agggcacggc gacatgtctg gaactacgcg
     2581 tactggctga ccggcgctgc cggtctggcc cttctggggg ggctggttgt cgccgtgttt
     2641 atcgagagct ggggagaaac cgtcctgcgc ccggacctct atgcgaacgc agattattac
     2701 ctgctgcagc cggctaacct gttcgggata gcctggtgtg ccggagggct tgtcgccggc
     2761 gtccttctgg cgcggctgct ggcgttcctg ctgcttgatg gtcattttgc ggccgctgac
     2821 gaggcggtga acgcgaaact gaatcaggag agccggcgca gcagccagcg gaccggagaa
     2881 atgacagacg tccgccacct gcatttcggg gagccggtac cggttaacgc gctggccgat
     2941 ttttccacgg agcaggcccg gaaacagcag gcggtgtttc tgggtaagga tgagcagggc
     3001 cagccggtgc tggtccctcg tgatacctgg cgcaagacca atattcagat cctcggcctg
     3061 cccgggagcg gcaagagcgc gatggccacc aacgcgctca ttcgctgcgt acgcgatttc
     3121 ggggacgctg tggtgtactt cgaccccaaa ggggacgcgt gggcgccgca cgtcttccgg
     3181 gcgcactgcc cggacttcac cctgctggat ctgcggccgg ggaaaccggc gcagctgaat
     3241 ctgttccgcg atctcgacca gtacgccctg aagaatctgc tggttgccgg gtttaacctg
     3301 agtgagaccg gcgacgtggc ggaccactac cgcatcagcg agcagaaagc ggcgaagctg
     3361 attgcggagc agttcccgca gggggcaaac attcagcagg tgctggcggc ggcgtacgcg
     3421 ttgccggagg ccctgaaaaa ggacgtgaag gggctgatca ccaagctgga gaacgtggcc
     3481 gacctgagcg tcctgcagac ggacagcggt atcgatgtgg cggggataat caacggcggc
     3541 ggctgcctgt acgtcatcgg ctcgatggac gatgaggcgg tgatccgggt gcagaagatg
     3601 ctgtttgccc gctgcgcgca aatcatcatt gcgcgggatg agttccggag gtggccgcac
     3661 gcgagcatca tgctcgatga gattaaatat ctgctgtcga agtacgtgct gaacgcgctg
     3721 gggacgctgc gcagccggga ctgcaacctg ctgctcgcgc accagtcgct gggcgacttc
     3781 ggtcagtgcg ggcaggatct gcccgctgat tttgtgaaaa ccacggtact ggataacacg
     3841 ccgatccgct ggttctatcg cgcggccagc caggagtccg cgcagtgggc ggcgggccag
     3901 accggggaga tccgggtgga cgtggagcgc cgccgggcca gccgggaggc ggggaacgtg
     3961 gagcacatca gcggcgacag cttcatccag aaggaggcgc ggccgctgtt tgacgtgaac
     4021 accctgcaac acctgcctga cgggttcgcg gtgatgaccg ggctgggcgt ggcgcggctg
     4081 gcgttcagca gcccgctgcg ggttgagcgc cgggaaattt cgctgaaaac cttcccggtg
     4141 ctggcgaaaa cggacccgct ggcggagtac ctgcctccgg ccgacggcgg cggaccaaaa
     4201 gcggatgatg attttgcggg gctttactga tgacactgga gcagtacaac gtcagccacg
     4261 cgaagcggca ggcgcggaac gcggagaaaa cacggctgac cctgcgctgg ctgcgggaag
     4321 agctgtgttc cacggcggag ctggttgcgc gacggctggg gattgcggcg gtacagccgg
     4381 tttaccgctt cctggacagt ctggtggcga aagggctgct ggtccgggcg aaatacccgg
     4441 ttgacggccg tcaggtcagc gtgtgggggc tgacgccgca cggggtggcg ttctcgttcg
     4501 acgaggacga gccgctgacg gacgttattc cgtttcagcc gtcaagggtg tcggcggcgc
     4561 agctgccgca ccggctggcc gtgcagtccc tgcgcctctc catggagggc cggggggcca
     4621 ccggctggcg gtacctgcac cgtatggccc tgaaagggat gaaagtgccg gacgcgctgg
     4681 cggagctgga cggcagaacg gtggcatttg aggtagagcg caccgtcaag agccgcaggc
     4741 ggtatcagga agtggtgtcc gggtatctgt tcaaccgccg ggcgaacggc atcgatgaaa
     4801 tctggtacat ctgccctgac cggccgacgc ttgtccgggt acagcgggcg attctggcgg
     4861 tggatgaaat cgtgaatcca cagaccggcg aggcccgcaa aacggtggag ctggaccggg
     4921 agaggctgtt cgcgtgcttt aaatttatga caacggagtg agggattatg gcgctgtaca
     4981 tggtatttat tgaggacgtg gaagacaaag acggcgagcg ctgcattatg ccaaccagcg
     5041 agcatctgga agacatggtg gtgctcgcct ggcagaccgg ggcgtatgtt ttctgttttg
     5101 tcgaccgggc ccagcatctg gcgatgatga aatccatcgg gatgtatgac gagcggctca
     5161 acagcgacga aaaagccacg tactttttca ccggtgaaaa tccgcaatgg gaacgggaca
     5221 aacccacagg aatgaagctg tgtgattacc tctatcgaaa ggtggagcgg ttcgccatcg
     5281 agaacggtgt gcaggtggtt gatgccggga agtgctcacc ggagcagatt aacgcgatga
     5341 ttgaggtggt cagcgagcgg cacggggaaa gcgcggtgaa cgagaagctg aaagcggagc
     5401 tgatgcggga atataacagc cgctatttta acgtgccggt gcctgaactg ccggaggaca
     5461 aacccgcccg ccggtggtgg ccgttcggca aaaaataacg ccagcaccgg catggccggt
     5521 gctgttattc ttccccgagc ttatcccgca tcacctgcca gagtgtttcc gcatcctcgt
     5581 gaagccgttc gcacatgtcg gccggggtat ccatgtccag ctcgttcagc ttttccagca
     5641 acagcagcga ctgatcctta atgaatttag ccatattgag ggcagttttt tcctgcttcg
     5701 tcatgttctc cattcccttc ggtaagttct cagctaaacc agccggacca gaagcgtttt
     5761 ttcttctgtg tcagttctgt tgtcaatctg tccacttcgg cctggagttt ccggcgctca
     5821 tcatcgtgct ggcggcgttc ttcttcccgg gatgttttat cttccagcat cagggtgaca
     5881 gcctgccgga gatcggccag ttccttttgc attgccagaa gctgttcctg tgggaaattt
     5941 ttttgtgtat tttcacggtg tgaaatgccg ggaatttgtt tctcagggat attcacattt
     6001 ccgtatacac ggatcagttc tgacacatcc acgacagggt tgtttttccc gtcacgtgac
     6061 acggtaacct ttccctgttt tacatgattg tatagcgttc ttctggtaat gcccgcggct
     6121 tgtgctgcct gtgaaaggtt gagcaatgtt ttcgccactg tatacccctg attttatcgg
     6181 gtgtgaaagg tgtgtaattt ctcacggtaa atgtatcgct gaattttacc cgtttttccg
     6241 ttcaggatgt ggttttctga cgcgctgcgc ttgtcaaacc ggcgacggcc acgcaatgaa
     6301 ttgcgccccc gtcatcggtt attagaggct gtctgccccc ggatacccgg attctttctg
     6361 atgcctgacg gtgacgatca caatctggtc catttcccgg tcgtggcggt agagcatcac
     6421 atagccactg tcgccaaacc cgatcaccag ttccttatat tccagcggca gaaacggcac
     6481 cgggcggcca atgtctggca atgttttcag ttgctggata gcccggacga tcacctcacc
     6541 tccttttctg gctgccagcc gatttttggt tttgagaaag tcctgaaggc gttgtaaatc
     6601 gcgctgtgcc agcgcggaaa tcgttacctg tggcatggcg gagcatcctg ctcgttctca
     6661 gttccccagg tacttatcca ggcttccgct tcttccgcag taaggtgcag acccgtttcc
     6721 tgatagtgct gccatgcggc ctgtccgtcc cgcaggtact ggctgcgctt ctcttcccgg
     6781 tcgatgtatt cggtgattgc ctccagcatt agcgcgtgcg ctgagcggtg ccggtcatcg
     6841 gccagcgttt tcagccggtc cttaagttct tcatcgagcc tgatggatgt tgctgatgac
     6901 ataaaagccc ctttgtagtc atatgtatta cacatgacta ctttaatccg tggcggcgct
     6961 atttgccaga aatacggctt ttgctgccca tttttcagct tcgcttccac taatcagggc
     7021 tgctacaggc tcactcatca tccaggacta ttgcagcctg cgggttaaag gacctgttac
     7081 tggcggcaac catcatcatt gagcttttgg gtggtgactg gtcgcggatc ctgtaagccg
     7141 tcatttttca gactgaattc aaaatttata acctgtcgca ttgccgggtg ccctgcggga
     7201 tatagccagt gcctcattgc cgtcagcgtt tcccgaaaaa aaaggtgaca gccggtaagc
     7261 gacacgttgt ccactcgtcc atcgtccaga atatcaaacg tggcggtcac ctgaccttcg
     7321 atgcgttttg ctgcggcctg taccgggtag gttggctgca gtgtggttac gggttttagt
     7381 ggtatcgcat gtaccggagt gaggaacatg gccgccagca ctgtcagagt aaaagctttc
     7441 atggtctgaa atccagaggg gcgtttttga atatttgcca ggtattttca tccggtgccg
     7501 gcggaatttt ggcgtgggta acggctgata ttgctgcttt acagagtttc ggatctcctc
     7561 cttcagcaat ggcgctgttc agagtgccat cacgttgtat ggatatccgc acggtgcaca
     7621 tttttcccgc ataggcctct ggttcaaaca tctgcatttc aacagcggtc ttgatggccg
     7681 cagcgtagct actgatttta gatgacttat ccagagtata aattttcggc ggctgggtgg
     7741 tggaatgctt acaggccgtc aggagtaagg ggctgcacaa tgccagcagg ataaagggta
     7801 ttaaacgcat cgggttgatg acctgtcagt aaaaaatgtt acatgtatat ttgtcactct
     7861 ttacggccac gttcatttac ataaacacgg cttatcgtat cgtttttaca ccaaacggcc
     7921 cgccatgcag ttgcgtgcat aggcatgcag cgggttacaa tttaccgggc gcgtttccgg
     7981 cggttttccg ggtggtttgt tgcctgtttt atcccgtagc cgccggaaac gccctcagac
     8041 cgtctgagcg gtgcgggcaa tgcgacgtta tggtaaatac cccttaccgg cggcgctgtg
     8101 gctgccaggt gaaggtggtt tccgtagcct cgtatttctc ccgacgcatt tcctgcgccc
     8161 gcctggcttc acgttcgtcc tgaatggcac gaagcaccct ttcccgcacc tgttgtttta
     8221 ccgcctttgg cagcgttttg ttccctttca ggcccatggc cagtgcctga gcgacaaagg
     8281 cgtccgccag gcgcttttct tcagcgggag tcagtttcag gtgcgtcata gtggcctccg
     8341 ggcgtctcag gtttcccgaa cagtttaacc gcaggtcctc tcctcatgcc agttagcttt
     8401 cagatgtgtc tataccatct gaaatgtaag tgtgatgaat gtcttaaata ataaaatttg
     8461 ttgccatgag aaattttcgg gaaaagacgc gatctgctta caggtggctc cgggcgcttt
     8521 tttgtgcctg aagatgagaa ctaatctcat atattgacac ctgaaaacgg gaggagtaag
     8581 gtaataatta tactgtgtat atgtacagtg tgcgccggga gaccggttag agatcaaggg
     8641 gtgaaagtcc ccgaccattg aaggaccagc aatccacaag gtccccgagt catgcgttgc
     8701 ataccgcgag gtatggggcg aagcgttgac aggggtgttg acaggccagc cattgagcca
     8761 cgaaatgtat attaaattac cgggtgccga cgttgtactg ttaacggaag gcaacatcat
     8821 agggtgcgat actgcgagtg ccacatggac ccggcggggt ctgagaccct ggcatgtcaa
     8881 tacgatctct acgcgggaac cgggagatct cccctctgac catctgccag tgtcggagat
     8941 ggcccgcacc gggaagacga ggagtcatag ccggtgatgt acggagagga gaagtcggac
     9001 tcgctcatag tagcggcgaa gcaggcgaac aacccgaaag gagcggagtc agtggagcga
     9061 aggagcgggg ccaaggggaa cgcggaacag ccacacatgc gccggacaca gagccgggaa
     9121 agcatgtcac agaggctgtc acgcgtgcgg gaagctgcga agcagcggaa gaaagaacgg
     9181 tttacagcat tgttccacct gctgacagtc gaagcactgg aagccgcatt cctctccctg
     9241 agcaggaaag cggccgccgg agtggatggc atcaggtgga tggactacgc cggaaacatg
     9301 aagaacaaca taacagatct gcaccggagg ctacatcagg gcagctacag ggcgcagccc
     9361 ggcaggcgtc actacatccc aaaagcggat ggaaaacaac gcccgctcgg catcgcctcg
     9421 ctggaggaca agatcgtcca gtatgcgctg gtgaaaatcc tgaacgcagt ctatgaaaac
     9481 gactttatgg ggttctcata cgggttcaga cccgggcgaa gccagcacga tgcactggac
     9541 gcactggcca cagggctggt acgcactaac gtaaactggg tactggatgc cgacatcagt
     9601 cagttcttcg acagggtgag ccacgaatgg ctgatcaggt tcacagagca tcggatcggc
     9661 gaccggaggg taatcaggct catacgtaag tggctcacag ccgggacgtc ggaggagggt
     9721 caatggcgag caacggagga aggcacccca cagggtgcgg tcatctcacc gctgctggca
     9781 aacatatacc tccactacgt cttcgatctg tgggcgcatc agtggcgacg tcgctatgcc
     9841 acaggcaatg tggtaatggt cagatacgcc gatgacatcg tcatcgggtt cgacaaacga
     9901 tacgatgccc ggcgcttccg tatagccatg cagcgcagac tgagggagtt cggactcacg
     9961 gttcacccgg agaaaacccg tctgatggag ttcggccgct tcgctgccga aaaccgtgcc
    10021 atcaggggaa aaggcaaacc agaaacgttc aacttcctcg ggttcacgca catcagcggg
    10081 aaagatcgca acggcaggtt catgctgata cgaaagaccc gccgggatcg gatgacggca
    10141 actctgaaag ccatcaaaga cggtctgcga aggcgctggc attactcaat ccccgaacag
    10201 ggaaaatggc tcaggagagt ggttcaggga tacctgaact atcactcggt accgggcaac
    10261 ttccccacca tgcagaagtt caggacacac gtaacaaacc tctggcgccg ggcgctcagg
    10321 cgcaggagcc agaaggatga tacgacctgg acgaaagcaa acaaactggc agccgcatgg
    10381 ctaccaaggg ttcgggttct tcatccatgg cctgtggagc ggttcaccgc cagacacccg
    10441 aggcaggagc ccggtgcgta aatcgcgcac gccgggatct gtgcgggggg tatccggtaa
    10501 cgggtatccc taccgcgaca tgttggcttt aatttattgt tttaaatgtc aaagaggaaa
    10561 acggtatgag tggtggagac ggtcgaggtc cgggtaattc aggtctggga cataatggtg
    10621 gtcaggccag tgggaatgtg aacggtacgt ctggtaaagg tggcccttca tcaggtggtg
    10681 gtacggatcc aaacagcggg ccgggctggg gtacgacgca tacgccgaat ggagatattc
    10741 ataactataa tccgggggag tttggtaacg gcgggagtaa acccggtggt aatggtggta
    10801 acagcggcaa tcatagcggt agctctggtg gcggacagtc ttcggccact gcgatggcct
    10861 tcggtctgcc tgccctggct actccggggg ctgaaggact ggctttatca gtttccggcg
    10921 atgcgttgtc ggccgccgtt gctgatgtgc tggctgccct gaaggggccg tttaagtttg
    10981 gtctgtgggg gattgcgatc tacggtgtgc tgccttctga gattgcaaaa gatgatccga
    11041 aaatgatgtc aaaaattatg acgtcattac cggccgatgc ggtgacggag actccggcaa
    11101 gtactttacc actggaccag gcgacggttc gtgtcagaca acgggttgtg gatgtggtga
    11161 aggatgagcg gcagcatatt gcggttgtcg caggtcggcc aatgagtgtc cctgtggtgg
    11221 atgcgaaacc gacaaaacgt ccgggggtat tcagtgtgtc gattccgggt ctcccgtctc
    11281 tgcaggtgag cgtaccgaaa ggtgttccga cagcgaaagc cccgccaaaa ggcattgttg
    11341 ctgaaaaagg tgattcacgt ccggctggtt ttacagccgg tggtaactcc cgtgaggccg
    11401 ttattcgttt cccgaaagag accggacaga agccggttta tgtgtcggtg acagatgttc
    11461 ttaccccggc acaggtaaaa cagcgtcagg aggaagaaaa gcgtcgccag caggcatggg
    11521 acgccgctca tccggaagag gggctgaaaa gagactatga taaagcgaaa gctgagctgg
    11581 atgccgaaga taaaaatatt gcgaccttaa acagccgcat tgcatcgaca gagaaggcgc
    11641 tccccggtgc aagggctgct gttcaggaag ccgataaaaa ggtaaaagag gcagaggcaa
    11701 ataaagatga ttttgtgact tataacccgc ctcatgaata tggctccggg tggcaggatc
    11761 aggttcgcta tcttgataag gatattcaga atcagaatga gaaattaaaa gcggctcagg
    11821 catctttaaa cgcaatgaat gaatccttat ccagagataa ggctgcactt accggggcga
    11881 tggagagccg gaaacaaaag gagaaaaaag cgaaggatgc agaaaataag ttaaatgagg
    11941 aaaagaacaa gcctcgcaag gggactaaag
//
