LOCUS       NZ_CP033959             5596 bp    DNA     circular CON 08-JAN-2024
DEFINITION  Klebsiella pneumoniae strain L39_2 plasmid p6_L39, complete
            sequence.
ACCESSION   NZ_CP033959
VERSION     NZ_CP033959.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN10433884
            Assembly: GCF_006494895.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Zheng,B., Yu,X. and Xiao,Y.
  TITLE     Carbapenem-Resistant Klebsiella pneumoniae L39 isolated from the gut
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Zheng,B., Yu,X. and Xiao,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2018) State Key Laboratory for Diagnosis and
            Treatment of Infectious Diseases, The First Affiliated Hospital,
            Zhejiang University, Qingchun, Hangzhou 310003, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2018
            Assembly Method                   :: HGAP v. 2.3
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 20.0x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/08/2024 04:56:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,947
            CDSs (total)                      :: 5,825
            Genes (coding)                    :: 5,647
            CDSs (with protein)               :: 5,647
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 68 of 178
            Pseudo Genes (incomplete)         :: 118 of 178
            Pseudo Genes (internal stop)      :: 24 of 178
            Pseudo Genes (multiple problems)  :: 32 of 178
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP033959.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="L39_2"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p6_L39"
                     /geo_loc_name="China:Zhejiang"
                     /lat_lon="29.8 N 118.2 E"
                     /collection_date="2018"
                     /collected_by="XuHao"
     gene            complement(1..267)
                     /locus_tag="EG820_RS30055"
                     /old_locus_tag="EG820_30425"
     CDS             complement(1..267)
                     /locus_tag="EG820_RS30055"
                     /old_locus_tag="EG820_30425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(251..475)
                     /locus_tag="EG820_RS30060"
                     /old_locus_tag="EG820_30430"
     CDS             complement(251..475)
                     /locus_tag="EG820_RS30060"
                     /old_locus_tag="EG820_30430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            complement(1142..1354)
                     /locus_tag="EG820_RS30065"
                     /old_locus_tag="EG820_30435"
     CDS             complement(1142..1354)
                     /locus_tag="EG820_RS30065"
                     /old_locus_tag="EG820_30435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1361..1870)
                     /locus_tag="EG820_RS30070"
                     /old_locus_tag="EG820_30440"
     CDS             complement(1361..1870)
                     /locus_tag="EG820_RS30070"
                     /old_locus_tag="EG820_30440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<1905..2609)
                     /locus_tag="EG820_RS31275"
                     /old_locus_tag="EG820_30445"
                     /pseudo=""
     CDS             complement(<1905..2609)
                     /locus_tag="EG820_RS31275"
                     /old_locus_tag="EG820_30445"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2548..2892)
                     /locus_tag="EG820_RS30080"
                     /old_locus_tag="EG820_30450"
     CDS             complement(2548..2892)
                     /locus_tag="EG820_RS30080"
                     /old_locus_tag="EG820_30450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(2882..3058)
                     /locus_tag="EG820_RS30725"
     CDS             complement(2882..3058)
                     /locus_tag="EG820_RS30725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            3698..3856
                     /locus_tag="EG820_RS31280"
     CDS             3698..3856
                     /locus_tag="EG820_RS31280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            4214..4540
                     /locus_tag="EG820_RS30090"
                     /old_locus_tag="EG820_30460"
     CDS             4214..4540
                     /locus_tag="EG820_RS30090"
                     /old_locus_tag="EG820_30460"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            4566..5111
                     /locus_tag="EG820_RS30095"
                     /old_locus_tag="EG820_30465"
     CDS             4566..5111
                     /locus_tag="EG820_RS30095"
                     /old_locus_tag="EG820_30465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            5177..5527
                     /locus_tag="EG820_RS30100"
                     /old_locus_tag="EG820_30470"
     CDS             5177..5527
                     /locus_tag="EG820_RS30100"
                     /old_locus_tag="EG820_30470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     Typing          complement(3145..3221)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="91.25"
                     /coverage="98.0"
                     /evalue="1.04e-21"
     Typing          3410..3807
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          complement(1027..2558)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.804"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tcagtcgtaa acttcgcggc gatgtccaat cgtggcggca aggataacaa gctcgtcatc
       61 tcggatctca cacagaacgc gataatctcc cacccgatag cgccagaact cgcccagctc
      121 acctttgagc ggcttccctg actggcgagg atcggcagca actgcaatgc gtaaactcat
      181 aaaatccaca atccgtcgtg cgttctgttt gtccatcttg cgtaacgatt tgagcgcccg
      241 atcggaatag ttaatcgtcc agaccaagtg acttcctcac gtcttcagac gaatgcactg
      301 cttcatcacc acggcgaacg cgtgcagcag tttctgctga aagataataa tcctccaggt
      361 cttccagatg cgccagtatt gcttcccgcg cataaaacgt ctttgttctg ccggtttgct
      421 tcgccagcga gtccagacgg gactcaatct catcagataa ccggatagca agcatataaa
      481 cctcccgaac accatttgct atacatgtat atcaacagta ccacaatcaa cctaaaagaa
      541 caaaaaataa tcttgttagc ccctcttgat aatccgtaac gcggtcaata tatctagctt
      601 gagggctttc ctcttaacta ttgaaaggat aacgattatg ggtcttaagc cgggtccgaa
      661 acgaattgcc gattcaacag gggaacctga taaacgccag cgtgacaata aaaaaacgcc
      721 gggaaatact gacaaattga aacccagcaa atcttcaaaa aaataagtca gtagttaaaa
      781 gccctccggg gcttttttgt atctgccgtt cacgctgatt aaacataaca cccgttgccc
      841 gcaccgcccc ggacgggttc gccgcgaacg gggtggcagt caggtaaaac cggcaacaaa
      901 ccacccgata aaccgccccg ttcgcgacag ataaatttta cctggctgca tagccatgca
      961 gccaggtgaa tgacatccgg tttcagcgta aatgcccgat gagaaccctg ttttcctgca
     1021 tttcagccca gcgacggacc gtcatagctt cgctgccggg aacgcgcttc cgtcagttcc
     1081 ttttcgtgct gccgttgctc gtgcgcccgt tctgcggcca cggccatctc atgacgctgc
     1141 actaccggct caaacgctgc agttgcccgc tcaagcgttc cacctgctga ctcaagcgcg
     1201 tcacgcgctc gctcagcgcc gcgttctccc gttgcataag ccctgacatt ttccgccatt
     1261 ccccgaaggc gctctcccac tcgtccagcc ttttcgagta gtcctgctgt agctgctcta
     1321 atgcgctcag caactgtttt tccagctcgg tcatcggcac ttactccttc accagtctca
     1381 gcgcgttctg cttcgaccgc ccgttcccga ccgtccagct ctgcgtcctg tctacggccg
     1441 tccccttcgg cagcaccaga aaacgtccgt tgctgtcttc caggtacgtc acgccccagg
     1501 tcttcgcgtt cagcttcgac agcgctgcgt tctgccggtc gatttcggcc agatttgacg
     1561 ctatcaagct cccctgatac cacaacaccc cgctcaggct ggcgaacagc agtcctgaca
     1621 ccatcgtgat ggtcagccat gtccgcccga ccatacgcat cacgctcagg cggttcgact
     1681 gcatggccgc gctcataccc tgctcgtggg cgcggatggc ggcgctgatt ctcttcccgc
     1741 tttcgctcag ttccgctctg acagacttct cgtgctcgct gaatgcggct ttcagcattt
     1801 cgccggtact ctgctgctgc gctttcgatt tctgctctaa gtccttcgcc agcgttaaaa
     1861 gactgttcat agatggctcc cttaagtcgg atgtttcgcc ctccgtccgg gtcggcaatg
     1921 ctgatgctgc tctttgtggt gcgcacgacc tcaaaacctg cgctttccag cgcctcagtg
     1981 acgtcctgac gcgttttaag ctcccctgac gaggcgaggg ccagtaagcc ccgcgtaata
     2041 gcctctgcgg cttcctgctt cgttttgggt aacgaagatg gcgtcaccag cgcgcgccgg
     2101 ttctccggtg cgttcgggtc gtgcagcccc agtcgcccgt tcaccacggt ctgccacgca
     2161 tcgatgcgcg gacggtctgc ccggtcgtag tacggctgga gacgcctgcc ggtcagcagt
     2221 tccgtgttcg gtaccagaaa attcagttcc agccgcccct tgtcccggtg ttcaacccac
     2281 agcacgctgt actggtcttt atcgagtccg ggcatcagaa cccgctcgaa gctcgccatc
     2341 agcttttcac gctggccggg cggtaaatcc tgttcagcaa aagacaggac gccggaggtg
     2401 tactttttgg cgtagggcga ggcgtcgata agctcccgga cctcctccgg ctttccctgc
     2461 aggacgatgg ccccgtcgcg ctggcggtct ttccccagca gataatccac cggcccggca
     2521 ccgccgcctc gccctcgcgg atgaaactta acgatcatcg tctgccccct tttccagcac
     2581 ggcatgccgc agccgctcga gttcggcatc aatggccatc agcgcggcca ccacctgaac
     2641 ccggtcagcg cctgaccact ggccgccgtt aattttccgg gcgatctggt tgaggttgtt
     2701 ccccatcccg gcaagctggc gcagcaaaac cggatcgatg gacggcagcc gcaacgaccg
     2761 cgccgggcgc gtatcgagac aggtttcccg catccacacc gccagttgcc ttccattgca
     2821 gcgctccagc agctgctggt actccccatc ggtcacccgg atacctaaaa aacgggtacg
     2881 cttaaccgcc atcctctcct ccgatatccg caaaatgtga ccgggctgaa cgctcaagct
     2941 ctgccatctg ctcccggcgg taatcttcct cctgtgcctg acgcatcaga tttgccatca
     3001 ctgccggatg ccaggatctt ttttttacga cgagcggctt cttcttcggc ttctgcatta
     3061 tccctccttc gcaaacgccg gacggcacgg aaaagttttc cgggtcgctc cggcgttgga
     3121 caagcgccag cgcgtcagtg gtgttagcgg gtttcggggc gcagccctga accagtcaca
     3181 gcgtttacgc tgtgtatact ggcttactat gttggcatga gagcacgatt catgaaagtg
     3241 cctcatgtgg caggtgtgat atgtggctca ggagcgtccg caataccatg cacggacaga
     3301 tgaaatgacg cctcctcgct cactcggtag ctgcgctcct gccgttcggc tgcggcgggc
     3361 gtgaccgact cacggataac gggataaagc aaaggcgctc aaatcagaaa gggccggaga
     3421 aaaggatgcg gcgttgccgt ttttccatag gctccgcctc ccctgacgag catcacagaa
     3481 atggacgctc aagtcagagg aggacgaaac ccgacaggac ttaaagatgc cggtgatttc
     3541 tctccggcgg ctccctcgtg cgctctcctg ttccgaccct gccgtttacc ggatacctgt
     3601 tccgcctttt tccctttcgg gaagcgtggc gctttctcat agctcacgct gctggtatct
     3661 cggctcggtt taggtcgttc gctccaagct gggctgtatg cacgaacccc ccgttcagcc
     3721 cgactgctgc gccctttccg gtaactatca tcttgagtcc aacccggacg gacacgcaaa
     3781 aacgccactg gcagcagcca ttggtaactg gatatatacg agagagatat tgagaggtct
     3841 tgaagtgggt gcctaactgc ggctacactg aaagaacagt tttggtatct gcggtctcgt
     3901 atacccagtt acccggttaa gcagttcccc aactgactta accttcgatc aaaccgcctc
     3961 cccaggcggt tttttcgttt acagtgcaag agattacgcg cagaaaaaaa ggatctcaag
     4021 aagatccttt aaatgatcgt cagttctgtt atccccggag ctccatccat cccacggaaa
     4081 aacacccgcc actgatgccc tcagcgcact gcacatcatg tacaaaccac tccctccctc
     4141 cttttttatt gcgtttctta atgcgcaatg gtagctttgt gcgcaacaaa aaacgcaata
     4201 gcaagggggg gtaatgagag aaagaacaaa tatcatgcta gatataggat taaagcaatc
     4261 cttacaacgg ctttcgaata aaaccaaaat gagtctaagt gatcttatta accagagatt
     4321 atcaggttca ctttcagttg aagaacgcat tggcgaaaag cctgaatgga tgagaaacct
     4381 tgatccctat ctcgccaatc acttttcaaa tattgagtta ccgctccctt caccattaaa
     4441 agaataccaa gaaaaaatgg atgagataga ttcaattatt aatgacatga tggttctaaa
     4501 ggaatatttc agagctaagt tttacgacga aataaaatag gcacgctata cggaggcgga
     4561 gaattatgtt ttttaaacgg tatttaacgg agaaggaaat atacaaggca gcactcccaa
     4621 ttgttaggct tcaagggaga gttaacactt cattacgttt tttatccaaa tactcagatg
     4681 taaacaaaaa tggattaatt gcattgcatc ttaatttatt acattttatc tcatacaaca
     4741 tacacggtgt agagtctctg aaaatcactc aagcaggcat aaacaaagct gtacaacaaa
     4801 gccctcacgc agatgaaata aaaaatattg cgcaggcaat aggacgcaaa ggacctgagt
     4861 acatcgaagg attaattaga aacccatggg ggaagaaaaa tgataatcca ctaatattta
     4921 taatgagcat tgaagaaagt ataaaagaaa cccttaactc aggttatatg ttatatagag
     4981 tagctcccat aactgagggt gaagcagctg ctttcatgga tgttcttaaa caattattat
     5041 tatttgagga taattttcgc ctttttggta gagacttggt ttataaaata gaaaaattaa
     5101 aaatcagata acaaagtatg gtgaacgaaa aatgaaagag aggatagtga aatgtgtaat
     5161 accaagattc tcaactatgt attggttaat tgcgttccta gcatttatat tccccggtcg
     5221 atcttttgct gacggttaca ccgcttacta ccgatgtaat ggtactcagg aagcatctct
     5281 tactgttaaa gataacaaac taagtatggt tgtaggtctt tctaagttta ataggcaact
     5341 ggatacttat agattcaaag aaaagggaat cgaagtagta atgcagagag ctgcttctga
     5401 tgataactct gaggccataa tgtcttttat tatgcctgac gttaacaata tgaccttatt
     5461 agttaaaaat aacggctaca ttatcgttaa gacaacctgc tggttaacta aaaccgacaa
     5521 acagtagaac acgtagttag tcgagtattt accataatac ctgttgtcgg catcaaaaaa
     5581 gcccgcatag cgggct
//
