LOCUS       NZ_CP033952             3998 bp    DNA     circular CON 26-NOV-2023
DEFINITION  Klebsiella pneumoniae subsp. pneumoniae strain ARLG-3135 plasmid p6,
            complete sequence.
ACCESSION   NZ_CP033952
VERSION     NZ_CP033952.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN10432823
            Assembly: GCF_003850165.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae subsp. pneumoniae
  ORGANISM  Klebsiella pneumoniae subsp. pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3998)
  AUTHORS   Fouts,D., White,R., D'Souza,R., Nguyen,K. and Singh,I.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-NOV-2018) Informatics Core Services, JCVI, 9605
            Medical Center Drive, Rockville, MD 20850, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: OCT-2018
            Assembly Method                   :: Unicycler v. 0.4.7
            Assembly Name                     :: ARLG-3135
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 87.5729x
            Sequencing Technology :: Illumina NextSeq; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/26/2023 02:29:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,462
            CDSs (total)                      :: 5,340
            Genes (coding)                    :: 5,192
            CDSs (with protein)               :: 5,192
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 15
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 67 of 148
            Pseudo Genes (incomplete)         :: 81 of 148
            Pseudo Genes (internal stop)      :: 24 of 148
            Pseudo Genes (multiple problems)  :: 21 of 148
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP033952.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3998
                     /organism="Klebsiella pneumoniae subsp. pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="ARLG-3135"
                     /host="Homo sapiens"
                     /sub_species="pneumoniae"
                     /db_xref="taxon:72407"
                     /plasmid="p6"
     gene            join(3675..3998,1..531)
                     /locus_tag="EG819_RS27950"
                     /old_locus_tag="EG819_27950"
     CDS             join(3675..3998,1..531)
                     /locus_tag="EG819_RS27950"
                     /old_locus_tag="EG819_27950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000586611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071579761.1"
                     /translation="MIIQEEHNIDFFKNQMPNKPYCTNNLDMGLSIRNKAKALEMLYLQ
                     ANQPAIQTCLLFDLDKKNSFYTFEQVGLPIPHFITKTPKTGRCHYGYMLKAGVCKTQQA
                     RLKPLKYAAAVEMAMAKKLKADLGFAGLITKNPLNDHWSPFWSGADLYDLDYLADFVDL
                     EKPQIQKKKSEAYGLGRNVNLFEDLRQYAYRTVLNFKKISTFEKFENELLLKAQGLNTF
                     CNPQNPLQYKEIKATVRSVARWTWKNFDSHTFSQIQSNRAKKPRKTNVKNEMLDILMSK
                     GVL"
                     /name="replication initiation protein"
     gene            528..791
                     /locus_tag="EG819_RS27955"
                     /old_locus_tag="EG819_27955"
     CDS             528..791
                     /locus_tag="EG819_RS27955"
                     /old_locus_tag="EG819_27955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001722510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_045287009.1"
                     /translation="MIINGKKIKAKEIMEMTGRSERTVRKYFSQSRDDYEKTAMDRRRQ
                     AYELRSQGLKWQQVADKMGCSYHGAVALYRRYVALDMPQNSL"
                     /name="hypothetical protein"
     gene            complement(1030..1314)
                     /locus_tag="EG819_RS27960"
                     /old_locus_tag="EG819_27960"
     CDS             complement(1030..1314)
                     /locus_tag="EG819_RS27960"
                     /old_locus_tag="EG819_27960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="WP_071579762.1"
                     /translation="MSKTTAQQIADLQNKLQALRTKKARQDRREETRQKIILGAEVAKV
                     LGKKPEEINKALILGLLSKINELDEEEKQEFTRLGLNILEEWKNENRKP"
                     /name="conjugal transfer protein TraD"
     gene            complement(1318..1701)
                     /locus_tag="EG819_RS27965"
                     /old_locus_tag="EG819_27965"
     CDS             complement(1318..1701)
                     /locus_tag="EG819_RS27965"
                     /old_locus_tag="EG819_27965"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071579763.1"
                     /translation="MNKLITEAEGAKIESVFKYILEASESILIEHGHVITGQIIKNKKF
                     FADNGHKDFERLEYYLSSAISSLVEDKVTEGIKDINTDENLVFKKINELSDKEKLILAE
                     CIIKHFEYSNVRGMQKAFNIFGG"
                     /name="hypothetical protein"
     gene            1945..3138
                     /gene="mobQ"
                     /locus_tag="EG819_RS27970"
                     /old_locus_tag="EG819_27970"
     CDS             1945..3138
                     /gene="mobQ"
                     /locus_tag="EG819_RS27970"
                     /old_locus_tag="EG819_27970"
                     /inference="COORDINATES: protein motif:HMM:NF041496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_076997031.1"
                     /translation="MATFHISFKKLRRSEGKSSVYLSAYQNREKTKDNRTGATWDYSKK
                     EGFFGSAILSPAGTPAELVKDSGTLWNAVEAGEKRKDAELCRYLDIAIPKELDDGQKKQ
                     IVLDYCQENFVDYGMIADIAFHDLDSHNPHAHVMLTLRTVSPNGFGNKQREWNNRENIE
                     AWRVNWEIIANDRLKKYGHKSRIDSRSLKDQKEEAERKAVFAKTPKEKAKWISKTIELD
                     RTPMTRIHRHQWRKGQKLRAMEQKEKALLLKIANATYLQQTKPPQPEETMKIEKKPLAR
                     SIIDNLKALYKKTQHIFKSKPNVNKNKKEEKEDPQNFVSSYVINEFGEHILKTELETKP
                     KTGTGTGTGGNNGGGKMGSGSGMSPMNQPQPKIEEPEPEPMPEFEDQQPKRKKKFGM"
                     /name="mobQ"
     Typing          1946..2955
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="85.0"
                     /evalue="0.0"
ORIGIN
        1 ttgaagtatg ccgcagccgt tgaaatggct atggcgaaaa aactaaaagc agatctgggg
       61 tttgctggat tgattacaaa aaacccactc aatgatcact ggtcgccgtt ctggtcaggt
      121 gctgaccttt atgacctgga ttatttagct gattttgtgg acttagaaaa gcctcaaata
      181 caaaagaaaa aaagcgaggc ttatggttta ggtcgcaatg ttaatttgtt tgaagatcta
      241 cggcaatatg cttacagaac ggttttaaat ttcaaaaaaa tatccacttt cgagaaattc
      301 gaaaacgaac tactattaaa ggcacagggc ttaaacactt tttgcaatcc acaaaacccg
      361 ttacaataca aagagataaa agcaacagtt agaagtgtag cccgttggac ttggaaaaat
      421 tttgatagcc atacattttc acaaatccaa tcaaacagag ccaaaaaacc tcgaaaaacc
      481 aatgttaaaa atgaaatgtt agacatatta atgagcaagg gggttttatg atcattaatg
      541 gtaaaaaaat aaaagcaaaa gaaataatgg aaatgacagg tcgtagtgaa agaactgtaa
      601 gaaaatattt ttctcaatct cgtgacgatt acgaaaaaac agcaatggat cgtcgtcgtc
      661 aggcttacga actacgaagc caggggctga aatggcaaca agtagccgac aaaatgggct
      721 gttcgtatca cggagccgtt gccctgtatc gccgttatgt tgccttagat atgcctcaaa
      781 acagcctgta acgacttcta agcagttttt ccccctaacc taaccaaacc ctccacccct
      841 ggggggattt tttccccttt ccccgtatcc gggccctgta tctcgaactg agaggcactc
      901 aggtacccaa caatcaacaa acccacccat aagccctgag agcctctctg agccttttta
      961 agacctccag gcaagcctgg caacccttca cagtaaaaaa ccagccaaga tcggctggtt
     1021 ttttattgtt cacggtttac gattttcatt cttccactct tctaaaatat ttaatcctaa
     1081 tcgtgtgaat tcttgttttt cttcttcgtc aagttcattt attttactta acaacccaag
     1141 aattaaagcc ttgttaattt cctccggttt tttacctaaa accttagcca cttctgcacc
     1201 caggattatt ttttgtcttg tttcttccct tctgtcctgt cttgcttttt ttgttcttaa
     1261 tgcttgtagt ttattttgca aatctgcgat ctgctgtgct gtcgttttac tcatccatta
     1321 ccccccaaaa atattaaatg ccttttgcat tcctctaaca tttgaatatt caaaatgttt
     1381 aataatgcac tctgctaaaa ttaatttttc cttgtcactc aattcattta tttttttaaa
     1441 aacaaggttc tcatccgtgt taatgtcttt aatgccttct gttaccttat cctcgacaag
     1501 agaactaatt gccgagctaa ggtaatactc taacctttca aaatctttat gcccgttatc
     1561 agcaaagaac tttttgtttt ttattatttg ccccgtaata acatgcccat gctctattaa
     1621 tatgctttca gatgcctcta atatgtattt aaaaacactt tcgatttttg caccttctgc
     1681 ctctgttata agtttattca taataacctc ctaattattt attttgttat ttattacctc
     1741 cattatttac tttagtcaaa tatattttag aataaaacaa ataatattta atattgggcg
     1801 aagcctgggg ttcattactt attattttga ggtgcaaccg aaaaaataat attagtaatg
     1861 cccacttaca cgtcgcttgc gacgtatggg ccctgcgggg ctaaaaacca aaattcaaaa
     1921 gacaaaaaaa gtaaggggta gactatggcg acatttcata tcagctttaa aaaattacga
     1981 agatcagaag gtaaatcttc ggtttattta tctgcatatc aaaaccgaga aaagacaaaa
     2041 gataatcgaa ccggggcaac ctgggattat tcaaaaaaag agggattttt cggctctgct
     2101 atcctctccc ctgccggcac acctgccgaa ctggtgaagg actcaggcac cctctggaat
     2161 gccgtagagg ctggcgagaa gcgaaaggat gccgaattat gccgttatct ggacatagcc
     2221 atccccaagg agctggacga cggccagaag aagcagatcg tcctcgatta ctgccaagaa
     2281 aatttcgtgg attatggaat gattgcagac atagcatttc atgatcttga tagtcataac
     2341 cctcatgctc atgtaatgct aactttaaga actgttagcc caaatggatt tgggaacaaa
     2401 caaagagaat ggaataatag agaaaacatt gaagcctggc gagttaactg ggaaataata
     2461 gcaaatgacc gattaaaaaa atatggtcat aaatctcgta ttgactcccg ttcattaaaa
     2521 gaccaaaaag aagaggcaga aagaaaggct gtttttgcta aaactccgaa agagaaagcc
     2581 aagtggattt caaaaactat tgaactggac agaacgccaa tgaccaggat acacaggcac
     2641 caatggcgaa aaggtcaaaa attaagagct atggagcaaa aagaaaaagc attattatta
     2701 aaaatagcta atgcaacata cttacagcaa accaaaccac ctcagccaga ggaaactatg
     2761 aaaatagaga aaaagcctct tgcaaggtca atcattgata atttaaaggc attatacaaa
     2821 aagacccagc atatatttaa atcaaaacct aatgtaaata aaaataaaaa agaagaaaaa
     2881 gaagatcctc aaaatttcgt tagtagttat gtaataaatg aatttggcga acatatatta
     2941 aagacagagt tagaaacaaa acctaaaaca ggcacaggaa ccggaaccgg tggaaataat
     3001 ggaggcggaa aaatgggttc aggctctgga atgtctccta tgaaccaacc acaaccaaaa
     3061 attgaagagc ctgagccgga accaatgccg gaattcgaag atcagcaacc taagaggaag
     3121 aagaaatttg gaatgtagta tatagtggta tatattccta tatagtagta tcacttaata
     3181 caacttaata ttatttaata ctatactata agatactaca tttttagagt tgtgatcctc
     3241 gccggttcta tttccccttt atgtattgcc acagcttcat taactgactt cataagcatt
     3301 ttaaaaaatt tactatgctt atttcttttc cgttttttct gcactttctt ttttttcata
     3361 tattcaccaa aaaaaatcga atcgccaatt tttcttcgaa caatactctt ttttcttttt
     3421 ttattgtttt tctatcttcg gtaatgcttc gcaccataga tctgatagta atgctttgct
     3481 gccgtatgta aagaactgcc ccaacttcgt tgggctgtta tctgatatgg ttagacgcac
     3541 acttgacaaa tttttgagat cggttagaac tggtacagga ttgaaaaatc tataaaaata
     3601 aaaaccccac cctctgcgat tttgttttgg cgacagaaaa cagagagtag ggccagataa
     3661 aaaggactga aaaaatgata atacaagaag aacataacat cgattttttt aaaaatcaaa
     3721 tgcctaacaa gccttattgt acaaataacc tcgatatggg attaagcata aggaacaagg
     3781 caaaagccct tgaaatgctg tatttacagg caaatcagcc agccattcaa acttgcttac
     3841 tctttgatct agataagaaa aattcattct atacatttga gcaggtgggc ttacccatac
     3901 ctcactttat tacaaaaaca ccaaaaacag gtcgttgtca ctacggctat atgctgaaag
     3961 ccggggtttg taagacacaa caagcaaggt taaagccg
//
