LOCUS       NZ_CP033951             4991 bp    DNA     circular CON 26-NOV-2023
DEFINITION  Klebsiella pneumoniae subsp. pneumoniae strain ARLG-3135 plasmid p5,
            complete sequence.
ACCESSION   NZ_CP033951
VERSION     NZ_CP033951.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN10432823
            Assembly: GCF_003850165.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae subsp. pneumoniae
  ORGANISM  Klebsiella pneumoniae subsp. pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4991)
  AUTHORS   Fouts,D., White,R., D'Souza,R., Nguyen,K. and Singh,I.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-NOV-2018) Informatics Core Services, JCVI, 9605
            Medical Center Drive, Rockville, MD 20850, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: OCT-2018
            Assembly Method                   :: Unicycler v. 0.4.7
            Assembly Name                     :: ARLG-3135
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 87.5729x
            Sequencing Technology :: Illumina NextSeq; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/26/2023 02:29:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,462
            CDSs (total)                      :: 5,340
            Genes (coding)                    :: 5,192
            CDSs (with protein)               :: 5,192
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 15
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 67 of 148
            Pseudo Genes (incomplete)         :: 81 of 148
            Pseudo Genes (internal stop)      :: 24 of 148
            Pseudo Genes (multiple problems)  :: 21 of 148
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP033951.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4991
                     /organism="Klebsiella pneumoniae subsp. pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="ARLG-3135"
                     /host="Homo sapiens"
                     /sub_species="pneumoniae"
                     /db_xref="taxon:72407"
                     /plasmid="p5"
     gene            397..563
                     /locus_tag="EG819_RS29615"
                     /old_locus_tag="EG819_27910"
                     /pseudo=""
     CDS             397..563
                     /locus_tag="EG819_RS29615"
                     /old_locus_tag="EG819_27910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505258.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            893..1951
                     /locus_tag="EG819_RS27915"
                     /old_locus_tag="EG819_27915"
     CDS             893..1951
                     /locus_tag="EG819_RS27915"
                     /old_locus_tag="EG819_27915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012508929.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="WP_042109829.1"
                     /translation="MAIYAVWNNKGGVGKSYLTFQLASEYARQNPTKKVLVVDLCPQAN
                     SSSMLLGGMINGENTLNQIHSAIQRKTISGYIEDRIRSPYIPTNTGSSYVINVSTHNSK
                     VPNNLYLVTGDEQLELQASRVVGASFPGPHDAWRTVHLWINDLISDICNSWNNEDSCVF
                     IDCNPSFSIYTELALTAAERLLIPFSADGSSKRAVKAVLSLVYGERRHQNEPQSDFYLN
                     SNRYSMSLPQIYMYVGNRLTQMNNSSASAFKTVVTEIGDEIWKVWQTKPSLFCIHPNGS
                     STPNNKRSFRNMFQYEVNDANTASVVSGALGIPIATLTAGNKTVSGKIIKVNQTQLDKQ
                     VPNIVALAGMIE"
                     /name="ParA family protein"
     gene            complement(1981..2238)
                     /locus_tag="EG819_RS27920"
                     /old_locus_tag="EG819_27920"
     CDS             complement(1981..2238)
                     /locus_tag="EG819_RS27920"
                     /old_locus_tag="EG819_27920"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_042109830.1"
                     /translation="MGSNNNQNKDLQDKQADLLDQTYTLGWMDGYAEGYDDAIEDTQNA
                     LNKLENENELLTEVVESKDNVIKELASSAGRALQAVKKNK"
                     /name="hypothetical protein"
     gene            2656..3087
                     /locus_tag="EG819_RS27925"
                     /old_locus_tag="EG819_27925"
     CDS             2656..3087
                     /locus_tag="EG819_RS27925"
                     /old_locus_tag="EG819_27925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="WP_042109831.1"
                     /translation="MKIPAHLKHKPIIQVENYDRIDGPHADDTDAMGLSVGIAQWNTPG
                     WTELSAKVWRNTGEKWSRQSEELPLHRVIDLATLICITMDYSKNGRLSSSDDFPVTRST
                     DNPELAHHTEFMKKELKQNKSNLDVSLKRLSTELKRLGY"
                     /name="DUF6530 family protein"
     gene            complement(3181..3585)
                     /locus_tag="EG819_RS27930"
                     /old_locus_tag="EG819_27930"
     CDS             complement(3181..3585)
                     /locus_tag="EG819_RS27930"
                     /old_locus_tag="EG819_27930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016529521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2251 domain-containing protein"
                     /protein_id="WP_008321396.1"
                     /translation="MTILVTIQAQLVVGEEQVIKSLAPEGMLAAVFEDDGRTGYFYALD
                     ESVEGNPIMDAVHIYNAEDVSDGHIPSDVKIGWSEDSQKCVLLINGYPHGAFDFVGKNG
                     YCRSGYPPPINKVWSLSGHEWSDSVDDFFR"
                     /name="DUF2251 domain-containing protein"
     gene            complement(4380..4781)
                     /locus_tag="EG819_RS27940"
                     /old_locus_tag="EG819_27940"
     CDS             complement(4380..4781)
                     /locus_tag="EG819_RS27940"
                     /old_locus_tag="EG819_27940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_032489494.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQLKGEHHSGQVSEQRDTASVDQCEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(4781..4972)
                     /locus_tag="EG819_RS27945"
                     /old_locus_tag="EG819_27945"
     CDS             complement(4781..4972)
                     /locus_tag="EG819_RS27945"
                     /old_locus_tag="EG819_27945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_032489493.1"
                     /translation="MTKQQKTALNMAKFIQHQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          103..505
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.857"
                     /coverage="99.0"
                     /evalue="7.72e-165"
ORIGIN
        1 gtgctcagtg tagggatgtt ctcttttccg cttcctcgct cactcagtcg ctacgctcct
       61 gccgttcggc tgcggcgagc ggcaggagca tgcgtatgaa agctcgagaa aaccgagcga
      121 tcacgtaatt tagaaaaggg aaggccgcac agcggccgtt tttccatagg ctccgccccc
      181 ctgacaagca tcacagaaac ggacgctcaa atcagtggtg gcgaaacccg acaggactat
      241 aaagatatca ggcgtttccc cctgatggct ccctcgtgcg ctctcctgtt cctgcctttc
      301 ggtttaccgg tgtcattccg ctgttatggc cgcgtttgtc tcattccacg cctgacactc
      361 agttccgggt aggcagttcg ctccaagctg gactgtatgc acgaaccccc cgttcagtcc
      421 gactgctgcg cctgttccgg taactttcga cttgagtcca acccggaagg cacgacaaaa
      481 cgccactggc agcagccact ggtaaggggt gtacaagaat tactggtgca gagttcttga
      541 agttacagcc taactacagc taaactgaaa ggacagattt ggtggcagcg gtcttgtaca
      601 ccctgttacc acggttcaga agttccccaa cttactgatc cttcgaaaaa ccacctcccc
      661 aggtggtttt ttcgtttaca gagcaaaaga ttacatcccg caggttagga tctcaagaag
      721 atccttttaa tcggaattcg acaactccag ataagtcatg gtgagccagc caaccccttc
      781 gactgaatat cgttccaaaa aaaggcagtc agctgaaaga aactaacagt agaacaataa
      841 tctctagtac cctgaatcta agctaacaac aaaactagat taggagttat agatggcgat
      901 ctatgccgta tggaataaca agggtggtgt agggaaaagt taccttactt ttcagttagc
      961 ctctgaatat gccagacaga accctactaa aaaggtcctg gtggttgacc tgtgcccaca
     1021 agcgaactcg tcctctatgt tacttggtgg aatgataaat ggcgaaaaca cactaaacca
     1081 gattcattca gcaatacaga gaaagacaat atctggatat attgaggata ggatcagaag
     1141 tccatatatt cctaccaaca ctggttctag ctatgtaata aatgtatcta cacacaatag
     1201 taaagttcca aataatctat atcttgtcac tggcgatgaa caattagaac tccaagcatc
     1261 aagagttgtt ggggcatcat ttccaggtcc ccatgatgca tggcgtactg ttcacctctg
     1321 gattaatgat ttaatcagtg acatttgcaa ctcatggaat aatgaagata gctgcgtatt
     1381 cattgactgc aaccctagct tctctattta tacagagtta gctttaaccg cagcagagcg
     1441 ccttttaatt cctttctctg ccgatggttc atcaaagaga gcagttaagg ctgtactttc
     1501 tttagtttat ggtgagcgtc gccatcagaa tgaaccacaa tcagatttct atcttaatag
     1561 caatcgttat tctatgtcgc taccacaaat atatatgtat gttggaaata gattaacgca
     1621 aatgaacaat tcatctgcca gtgcatttaa gacggtagta accgaaattg gtgatgaaat
     1681 ttggaaagta tggcaaacaa aaccaagtct gttttgtatt cacccaaatg ggagctcaac
     1741 gccaaataat aaaagatctt tcaggaatat gtttcagtat gaagtaaatg atgcaaacac
     1801 agcatctgtc gtatctggag ccttaggtat tccaatcgcg acattaactg caggaaataa
     1861 aactgtttca ggaaaaataa taaaggttaa tcagacgcag ctagataaac aagtccccaa
     1921 cattgtagct ctcgctggta tgatcgaata aaatagcaag gcccttaaaa gggccttttt
     1981 ttatttgttt ttctttactg cttgcagcgc cctgccagca ctagaggcca actctttgat
     2041 tacattatct ttactttcaa caacctcagt aagcaactcg ttttcgtttt ccagtttatt
     2101 caaagcgttc tgagtatctt caatagcatc atcataccct tcggcatagc catccatcca
     2161 accaagagta tacgtttggt ccaaaagatc agcttgctta tcctgcagat ctttattctg
     2221 attattgtta gagcccatgt tttttcctca tttgttggcg catctgagtt ttcaaaccag
     2281 gaggaattcc atttctaagt ttctcactta gtctgactgg tggtgtgttt cctgagactg
     2341 gattacagtc agcatccacc ccgacatctt tctttttata gtcgccaata ttcctaacgt
     2401 tatctgtagt catagaaaac cacgcccatt gctgtgaata accgaactat aaaccagaaa
     2461 aaaaacgtac ggcttatcat aacacttgac acaaaaaaac accgctactc tccaagcaaa
     2521 aaacataaaa ttggaaaaac aaaatcacaa gcacatgata acaatagata ttaatattta
     2581 cttacattta gtgagtttta taattagaaa aacaatgtaa tttaataatc acaaacatta
     2641 cctaaggacg aactgatgaa aatacccgcg catttgaagc ataaaccaat tattcaagtc
     2701 gaaaattacg accgcattga cggcccacat gccgatgaca ctgatgctat ggggctatct
     2761 gtaggtattg ctcaatggaa cactcctgga tggactgagc tttcggcaaa ggtttggcga
     2821 aacacaggag aaaaatggtc aagacaatca gaagagctgc ctcttcaccg ggtaatcgac
     2881 cttgcaactc tgatctgtat caccatggac tacagtaaaa atgggcgact ttcatccagt
     2941 gatgattttc ccgtgactcg ctcaactgat aaccctgagc tagcacatca cactgagttt
     3001 atgaaaaaag agttaaaaca aaataaaagc aatcttgatg taagcctcaa acgcctctcg
     3061 actgaattga agagacttgg ctattaaaac tagctttaga aatgtctacc tacttcacag
     3121 acgaatttac cataacatcc gtttttcgaa ccataatggc aggctatagc ctgccgtaat
     3181 tcaccggaaa aaatcatcca cagagtcgct ccactcatgc ccggaaagag accacacctt
     3241 gtttataggg ggaggataac cactcctgca gtacccgttt tttcccacga aatcgaatgc
     3301 cccgtgcggg tatccattga tcaggagtac gcacttctga ctgtcttctg accagccaat
     3361 tttaacatca gaaggaatgt gaccgtctga aacgtcttcg gcattgtaga tgtgtactgc
     3421 gtccataatc gggtttccct cgacggactc atcgagcgca tagaaatacc cggtccggcc
     3481 atcgtcttcg aacacggcgg ccagcatgcc ctcaggagcg aggctcttta taacctgttc
     3541 ctccccgaca acgagctggg cctgtatcgt cactaaaatg gtcatcaggt ctgttctcct
     3601 ttccttggtt gcttcggcag ccagctttca gacaagcgga taatttacca taacatccgt
     3661 tatcagcatc gtcagaaacg cgctgagggc gtttctgacg ggaaaccggt aaaacaggca
     3721 acaaaccacc cgaaaacccg ccagaaacgc gcaggctctg tttttatggc atgcatgagt
     3781 atgcacgcaa gtgcatagcg cgaatattca cgccattcag acagggtaag tcctgtttcc
     3841 ggcgttcttc ccatccgaaa acggtgaacg gggacgcaat ttattgcgtg gccgtgaagc
     3901 gttgccggcg cagccggaaa accggttttc agcacgttca ggaatcgggt tcgtcgacgg
     3961 ccggcaatca cgttatggca agtcgccgga cgactcacca cacgattcac cggcgactct
     4021 gcactagagt cgtacctaga gtcgccgcga ctaaaaaaca accaaacggg gagtcgggtc
     4081 tagagtcggg tcgttgttaa cttattgttt ttatagtcct gagtcgttac cgactctatg
     4141 acgactcgcc ggggactcta tgacgagtcg tcatagagtc ggatctatat aaataaaatt
     4201 taaaaaacga aatctcagga aagtaaacta ctgatattaa atacaaatct gcaaaatttt
     4261 aattttgcgt ggggtgtggg tatttttagt ggtgaggagc gttcagagat ctgactattt
     4321 ttgggaaggt tcaggaactc cccgggatgg ccggagagga aaactgaagc atgacagggt
     4381 caggattttt tccagaattt ccaccatggc gccggggatt cagttaattt tgcctggtga
     4441 ttttcaagaa ggagcatggc ctgtttcagg ctgtcaatgt gcgaatcttt ggacaaaagt
     4501 aaagtgttca ggtgttcaat ctcctgtttc agatgacgaa tcacctgttc acactggtca
     4561 acagaggctg tatcgcgctg ttcagagacc tgtccagaat ggtgttcacc cttcagttgt
     4621 tcaaagggac caaagacacg taaaagctct gaagtatcta ccatttttgc attgcgctca
     4681 ccgctacagg tggacaggtc acctgcttta attaacctgt gcagtgttgt tctactctta
     4741 cccgtcagtc gggctgcctc actaatagac actctggcca tcagttacca tcctgtaatg
     4801 catccagtct cgaggacagt gtacggaaaa gatgctccgc atcatcgtgg agtttctcac
     4861 aaaggtcggc ctcgacatca agatcaagct cgttaagctt ctccagcagg agcagagatt
     4921 gatgctggat gaatttagcc atgttcagcg ctgttttctg ttgtttcgtc ataagaggcc
     4981 tcagagcgtc a
//
