LOCUS       NZ_CP033949             7819 bp    DNA     circular CON 26-NOV-2023
DEFINITION  Klebsiella pneumoniae subsp. pneumoniae strain ARLG-3135 plasmid p3,
            complete sequence.
ACCESSION   NZ_CP033949
VERSION     NZ_CP033949.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN10432823
            Assembly: GCF_003850165.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae subsp. pneumoniae
  ORGANISM  Klebsiella pneumoniae subsp. pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 7819)
  AUTHORS   Fouts,D., White,R., D'Souza,R., Nguyen,K. and Singh,I.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-NOV-2018) Informatics Core Services, JCVI, 9605
            Medical Center Drive, Rockville, MD 20850, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: OCT-2018
            Assembly Method                   :: Unicycler v. 0.4.7
            Assembly Name                     :: ARLG-3135
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 87.5729x
            Sequencing Technology :: Illumina NextSeq; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/26/2023 02:29:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,462
            CDSs (total)                      :: 5,340
            Genes (coding)                    :: 5,192
            CDSs (with protein)               :: 5,192
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 15
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 67 of 148
            Pseudo Genes (incomplete)         :: 81 of 148
            Pseudo Genes (internal stop)      :: 24 of 148
            Pseudo Genes (multiple problems)  :: 21 of 148
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP033949.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7819
                     /organism="Klebsiella pneumoniae subsp. pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="ARLG-3135"
                     /host="Homo sapiens"
                     /sub_species="pneumoniae"
                     /db_xref="taxon:72407"
                     /plasmid="p3"
     gene            1..114
                     /locus_tag="EG819_RS27815"
                     /old_locus_tag="EG819_27815"
     CDS             1..114
                     /locus_tag="EG819_RS27815"
                     /old_locus_tag="EG819_27815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071789001.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPVRHAKAPLAAATGN"
                     /name="replication initiation protein"
     gene            complement(483..1603)
                     /locus_tag="EG819_RS27820"
                     /old_locus_tag="EG819_27820"
                     /pseudo=""
     CDS             complement(483..1603)
                     /locus_tag="EG819_RS27820"
                     /old_locus_tag="EG819_27820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611476.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /name="IS3 family transposase"
     gene            complement(1792..2784)
                     /locus_tag="EG819_RS27825"
                     /old_locus_tag="EG819_27825"
     CDS             complement(1792..2784)
                     /locus_tag="EG819_RS27825"
                     /old_locus_tag="EG819_27825"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.3"
                     /GO_function="GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="WP_087874902.1"
                     /translation="MNKSIESLQVIRAIAACSVVLTHIYGASVPVIGSSGIFGVDLFFV
                     LSGYLMTITYRKNRTGYQFIKSRILRIYPPYLVLSLPLIIFSYFYKNISASDIFHNITL
                     LPTIHGNYDRINFVCWTLTYELYFYIVFSLCMYIFKGARNVILSTSFTMLVLFFLAGGV
                     NGGLAGWKDNSIHHMIGNYLILDFILGSLIGLAYNGYTYKKNAFPILLSIAIIITSCTI
                     IGHIHLPEPYNNYQLITFLTSGIPACVVIYITSKVSVDKIPLKKALIYIGTASYSIYLT
                     HLYAVFAFSKLTHNNAPEKFLCFVTSIVIGCVAYYTIERKIIIKKKNHV"
                     /name="acyltransferase"
     gene            complement(2871..3568)
                     /locus_tag="EG819_RS27830"
                     /old_locus_tag="EG819_27830"
     CDS             complement(join(2871..3319,3319..3568))
                     /locus_tag="EG819_RS27830"
                     /old_locus_tag="EG819_27830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558270.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1X4 family transposase"
                     /protein_id="WP_223335786.1"
                     /translation="MASISISCPSCSATEGVVRNGKSTAGHQRYLCSHCRKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGCRASARIMGVGLNTILRHFKKLRPQSVNSRIQPGSDVIV
                     CAEMDEQWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERTLATLERLLSLLSAFEVVVW
                     MTDGWPLYESRLKGELHVISKRYTQRIERHNLNLRQYLARLGRKSLSFSKSVELHDKVI
                     GHYLNIKHYQ"
                     /name="IS1-like element IS1X4 family transposase"
     gene            3852..3962
                     /locus_tag="EG819_RS29080"
     CDS             3852..3962
                     /locus_tag="EG819_RS29080"
                     /inference="COORDINATES: protein motif:HMM:NF033853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein"
                     /protein_id="WP_139102090.1"
                     /translation="MDWISLAIAIALLVVAVYSGISYFREKKTISRVFRR"
                     /name="small membrane protein"
     gene            5016..5237
                     /locus_tag="EG819_RS27835"
                     /old_locus_tag="EG819_27835"
     CDS             5016..5237
                     /locus_tag="EG819_RS27835"
                     /old_locus_tag="EG819_27835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016947202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016162091.1"
                     /translation="MEFDTAAELAALQAQTRRIRQVRYRPSRLDRYTGELLSLYQAGAS
                     AAELQRWLRARRIKVVLSTVTRWLEKNG"
                     /name="hypothetical protein"
     gene            5230..6117
                     /locus_tag="EG819_RS27840"
                     /old_locus_tag="EG819_27840"
     CDS             5230..6117
                     /locus_tag="EG819_RS27840"
                     /old_locus_tag="EG819_27840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016947203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="WP_071579754.1"
                     /translation="MAKFASAARQAAGVIKPLQGNILRSVGTVRNYEQGLTRVAEWMKF
                     NRINHGDLRTLTPAQAITYLEQRGEQVGQKTLDMERQAIQAMMIHVTQTLPAGEHLPVV
                     KSEQAQILSSRAYSPDQVALIAAAQQPANALATAIALAAGLRAHELLTLAPHQERAPDP
                     RPAADSKFAGREGVIYTVQGKGGLVREVLLPSRLAERLEHVRLASPVRVTDRGVFYQSQ
                     YAIGGGQRWSNAFSAASMRTLGWSRGAHGVRHSYAQQRMQELQKLGLVRDMALRTVSQE
                     MGHFRPEITETYLR"
                     /name="integrase"
     gene            6114..6812
                     /locus_tag="EG819_RS27845"
                     /old_locus_tag="EG819_27845"
     CDS             6114..6812
                     /locus_tag="EG819_RS27845"
                     /old_locus_tag="EG819_27845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016947204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071579755.1"
                     /translation="MTRTFILLCTGVFAIAYALYELKHMGVYYPDASNPQLFTGWKAVT
                     ELGPAILATGIAATAFSLWLFILFFRGSESRRIQSETDRYKQALREAEEAHRARQNTLI
                     QNHNLETAALRSHYSGAEDVLREAFAQKESLLLQAVQNARKEAKQELEEQREALNQRES
                     ILQKKELALETTLEKARKYVAGERHNTVLARLEAEKAEERRRNACATAERRRRKLEKLQ
                     TTDGTAGQKH"
                     /name="hypothetical protein"
     gene            complement(6819..7217)
                     /locus_tag="EG819_RS27850"
                     /old_locus_tag="EG819_27850"
     CDS             complement(6819..7217)
                     /locus_tag="EG819_RS27850"
                     /old_locus_tag="EG819_27850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016162088.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_071579756.1"
                     /translation="MANVSISEAARLTGKSRTTLHRLIKAGELSTCRGERNAKMLDTSE
                     LLRFFGAFAPVSVEQEEEQVSGQRDTVTSDHNEQAIYILKQEIEHLNTLLASKDSHIDS
                     LKQAMLLLENREEKRQNTNRPWWRFWQS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(7217..7408)
                     /locus_tag="EG819_RS27855"
                     /old_locus_tag="EG819_27855"
     CDS             complement(7217..7408)
                     /locus_tag="EG819_RS27855"
                     /old_locus_tag="EG819_27855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_071579757.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDAGADLCEKLHDDA
                     EHLFRTLSLRLDALQEGD"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          2..114
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.491"
                     /coverage="100.0"
                     /evalue="5.74e-46"
     Typing          7457..7819
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.556"
                     /coverage="92.0"
                     /evalue="8.16e-121"
ORIGIN
        1 atgcacgaac cccccgttca gtccgactgc tgcgccttat ccggtaacta tcgtcttgag
       61 tccaacccgg taagacatgc aaaagcgcca ctggcagcag ccactggtaa ctgatttaga
      121 ggagatagtc ttgaagttat gcgccggtta aggctaaact aaacgaacag ttttggtgac
      181 tgtgctcctc caagccagtt acctcggttc aaagagttgg tagctcagag aaccttcgaa
      241 aaaaccgccc tgcaaggcgg ttttttcgtt tactgagcaa gagattacga tgctaaagaa
      301 aggatctcaa gaggatcctg ttccatatgc ctacccctga agtgaaacca gcaccactcc
      361 gcgacaccaa ccatcaaaat aaaactattt aactcgttgt ttttgataag aaaaaaacat
      421 atataatcat gatccataat catccaacgc ggttgacctg cccccaggat tagatacaac
      481 cttcagttag taatgtcggt tcgtttttct tcatatttcc cgtttcgcca gcccgccgca
      541 aattcagccg gagtctggta gttcagcgat gaatgtggac gacactcgct ctaatcctgc
      601 ggccagtcat taatcgtttt cctcgcgtga acgatatcgc ggaaccagtg ctcattgagg
      661 cactcatccc tgaatcgtcc gttaaaactt tcaataaatc cgttctgtgt tggcctgccc
      721 ggctggataa gccgcagctc caccccatgc tcataagccc actggtcgag tgctctgcag
      781 gtaaactccg ccccctggtc agttcttatc gtcgccggat agccgcgaaa cagtgcaatg
      841 ctgtccagca ttcgcgtgac ctgaacgcct gaaatcccaa aggcggcggt aattgtcaga
      901 cactccttcg tgaagtcgtc cacgcaggtc aggcacttga tcctgcgacc ggtggccagc
      961 gcgtccatga caaaatccat cggccaggtc aggttgggcg cctccgggcg gagcagcggc
     1021 agacgttctg ttgccagacc tttacgacgt cgtctgcgtt ttacacccag cccgttaaga
     1081 tggtaaagac ggtacacgcg cttgtgatta acatgaaggc cttcacggcg cagtaactgc
     1141 cagatgcgtc ggtagccaaa acgcctgcgc tccagtgcca gctcagtgat gtgccctgat
     1201 aaatgcgcat cagccgcggg acgctgagcc tcatagcggc aggtcgacag ggacaaacct
     1261 gtaagcctgc aggcacgacg ttgcgacaga ccggtcgcat cacacataaa ctcaacggct
     1321 tcccgtttct ggtctgtcgt cagtactttc gccccagagc cacctgaagt gcctccttat
     1381 ccagcatggc ttcggcaagc agtttcttga gtctggcatt ctcttcctca agcgacttaa
     1441 ggcgcttaac ctcgggcacc tccataccgc catacttctt acgccaggtg taaaagatgg
     1501 cgtcggaaat ggcgtgctta cggcagagct cacgggcaga aaccccggct tcggcctcgc
     1561 ggagaatact gatgatctgt tcgtcggaaa aacgcttctt catgaggatg tcctcatgtg
     1621 gcttatgaag acattactaa catcacggtg tattaaccaa cggggagcag gtcactccgt
     1681 gtatgccccg gttttttcgc gctgtcgctg tctaaactgg ctgtaaaaat ttacgcctac
     1741 tgggatgggc tcgcagtagg ttcattaacc aattattaaa tgctcatgtt atcaaacatg
     1801 gttctttttt tttattatta tcttcctttc tattgtgtaa tatgcaacgc agcctattac
     1861 tatcgaagta acaaaacata gaaacttttc aggtgcgttg ttatgagtta gtttagaaaa
     1921 tgcaaacaca gcgtaaaggt gtgttagata aatggagtat gaagctgttc ctatataaat
     1981 caatgctttc tttagaggta ttttatctac acttaccttt gatgttatgt atatgactac
     2041 gcatgctgga atcccagatg ttagaaatgt aatgagttgg tagttattat atggctcagg
     2101 taagtgaatg tggcctataa tggtgcaact cgtgattatt attgctattg atagcaatat
     2161 agggaatgca ttttttttgt atgtgtaccc attgtacgcc aatccaatta aggagccaag
     2221 tatgaagtct aatattagat agttaccaat catgtgatga atagaattat ctttccagcc
     2281 cgctaatccg ccgttaactc cacccgctaa aaaaaataaa acaagcatgg tgaatgaagt
     2341 ggataagata acattccttg cccctttgaa tatgtacatg cacaatgaaa atactatgta
     2401 aaaatataat tcatacgtta atgtccagca aacaaagttt atcctatcat agttaccatg
     2461 aatagttggg aggagggtta tgttatgaaa aatatcagat gcacttatgt ttttatagaa
     2521 atatgaaaaa ataataagag gcagtgataa cacaaggtaa ggtggatata ttcttaatat
     2581 tctgcttttt ataaattgat accctgttct gtttttgcgg tatgtaatag tcattaaata
     2641 accgcttagc acaaaaaaca gatcaacacc aaagataccc gaagagccaa ttacaggtac
     2701 gcttgcgcca taaatgtgtg taagaactac tgaacaggct gctatggctc gaataacttg
     2761 tagtgactcg atacttttat tcatgaattt acatactgaa atgggttatt gaggtatttt
     2821 actggaaaaa actggatgta tcactaatta cttgtggtga tgacttcaac ttactgatag
     2881 tgttttatgt tcagataatg cccgatgact ttgtcatgca gctccaccga ttttgagaac
     2941 gacagtgact tcctgcccag ccttgccaga tactgcctca gattcaggtt atgtcgctca
     3001 atgcgctgcg tatatcgctt gctgataacg tgcagttctc ccttcaggcg tgattcatac
     3061 agcggccagc catccgtcat ccataccacg acctcaaagg ccgacagcag gctcagaaga
     3121 cgctccagtg tggccagagt gcgttcaccg aacacgtgcg ccacaaccgt cctccgtatc
     3181 ctgtcatacg cgtaaaacaa ccagcgctgg cgtgatttag cgccgacgta accccactgt
     3241 tcgtccattt ccgcgcaaac aatgacgtca ctgcccggtt gtatgcgtga gtttaccgac
     3301 tgcggcctga gttttttaag tgtcgtaaaa tcgtgttgag gccaacgccc ataatgcgtg
     3361 cactggcgcg acatccgacg ccattcatgg ccatatcaat aattttctgg tgtgtaccgg
     3421 gctgagaggc ggtgtaagtg aactgtagct gccatgtttt acggcagtga gagcagagat
     3481 agcgctgatg tccggcagta cttttaccgt tacgcaccac gccttcagta gctgagcagg
     3541 aaggacaact gatggagatg gaagccacgg gagcacctca aaaacaccat cgtacactaa
     3601 atcagtaagt tggcaccatt accgtgattt gttatatacc caccacgggg gcattagttt
     3661 ttttattttt ttgatgatat gtacaaaatc agtggttagg aataatctta gcagccttaa
     3721 caaacacata caaaaacagc ggttatgttt catctcacca caataagtaa cgacagtatc
     3781 atgctaaaaa aagatgacat tatctttatg aaatcataac aggctgtttt tattaaatta
     3841 atcaggcatt tatggactgg atttcacttg ctatcgccat tgctctactt gttgtagctg
     3901 tctactcagg aatctcatac ttcagagaaa aaaaaacaat cagccgtgta tttaggcgat
     3961 agcgccctat ttaccataat cccttcttac gcgcaccgcg aaaacggcct cagggcgttt
     4021 ctggtggtta acgggcaaaa ggtggtggca agtacaccga aaaccgccag aaacgcgcct
     4081 gatttatttt acgcatatgc atggctatgc acaccaccgc atagccggtg atttcggccg
     4141 agaaaagggt tctggacgtt aaaagcataa aagaacgggc catccaggcc cgtcaaatga
     4201 ggacaactct gtaaaaggac cgtcgcacct ggtgcggtgc cggcgacaag aaaaagatag
     4261 ctcatatcag cccctcaccg gagaaaaata tttcctggcc acctcaaccg ccacacgctg
     4321 tgttcccgtt aactcgcgca gataacgcag gaacatcctg ttttcctctg catgcgcctc
     4381 ctcactgccc tgtgcgtggt attccactat cgcgttcacc acggactcac cgacctgcat
     4441 cgtctgacct catctgcact ttcatcacac cgtttcagat cccgcctcct cgccatgatg
     4501 tgaaacggtg accgggcagg caaatttttt gtctgtccgg tcaccgttgg cggggtccgg
     4561 ggcggtcagt cccggtgttt tttattccgc caaatcagca cctgcgacac ccgctatgta
     4621 cgacgcataa aatgcgcctg tacatagcgg gtgtcgctat taaactgagt caggcgcaac
     4681 atttaaaaaa caacattcac acgcacgaaa aacctggcta gtatttagtc aaaccatgag
     4741 gaggcgcact ctgacgcgca cctgcctacc ggcagcaggt aaaacagccg ggtccccccg
     4801 gggatactgt accggacagc ccaatccgag aatcctgaga aaacagccaa aatctgcgag
     4861 accggcaatc aggtcaggcc attccctggc ttaagggacg tttttacagc cgcgagcagt
     4921 ccgggcttaa ccacgttacg gtttaaacga gggcgatcgg cgcaggccgc agtggatttc
     4981 cactggcgtt cttacaactc aacaccggag ttcctgtgga atttgatacc gccgccgaac
     5041 tggccgccct gcaggcgcag acccgccgta tccgccaggt ccgctaccgt ccctcccgcc
     5101 ttgaccgcta taccggcgaa ctgctgagcc tgtaccaggc cggggccagt gcagcggagc
     5161 tgcaacgctg gctccgggcc cggagaatca aagtcgtgtt gtccacggtt acccgctggc
     5221 tggaaaaaaa tggctaaatt cgccagcgct gcccgccagg cggctggcgt gataaaacca
     5281 ctccagggaa atattctgcg ctctgtcggt acggtccgga attacgagca gggactgacc
     5341 cgcgtcgcag agtggatgaa atttaaccgc atcaaccacg gtgatttacg cacactgaca
     5401 ccggcgcagg ccatcaccta cctggagcag cgcggggagc aggtcggcca gaaaacgctc
     5461 gatatggaac gccaggccat ccaggccatg atgatccacg tgacgcagac cctccctgcc
     5521 ggcgaacacc tgccggtggt gaaatcggag caggcacaga tcctgagcag ccgggcctac
     5581 agcccggacc aggtggccct gattgccgca gcacaacagc ccgccaacgc cctggccacc
     5641 gcgatcgccc tggccgccgg attacgcgcc catgaactgc tgaccctcgc gccccaccag
     5701 gagcgtgcac cggatccccg tccggcagcg gacagtaaat ttgccggtcg ggagggtgtg
     5761 atttacaccg ttcagggcaa aggcggcctg gtcagggaag ttctgctgcc gtcacgcctg
     5821 gctgaacgtc tcgagcatgt caggctggcc agcccggtcc gggtgaccga tcgcggcgtg
     5881 ttttaccaga gtcagtacgc cattggcgga ggccagcgct ggtcaaacgc cttcagtgcc
     5941 gcctccatgc gtaccctggg ctggtcacgg ggggcacacg gcgtgcgtca cagctacgcc
     6001 cagcaacgga tgcaggagct gcaaaaactc ggtctggttc gtgatatggc cctgcgtacc
     6061 gtgagccagg aaatgggcca ttttcgtccg gaaatcacgg agacatacct gcgatgacaa
     6121 gaacgtttat tttattgtgt accggtgttt tcgccatcgc gtacgccctg tatgagctga
     6181 aacatatggg cgtttactat ccggatgcca gcaatccgca gctgttcact ggctggaagg
     6241 cggtcaccga actcggtccc gcaatcctgg ctacggggat cgcagccaca gcgttctcgc
     6301 tgtggctgtt cattttgttt ttcagaggct cagagtcccg ccggatacag agcgagacag
     6361 acaggtataa gcaggctctg agggaggcag aagaggctca cagagccaga caaaataccc
     6421 ttattcagaa tcacaacctg gagacagcgg ccctgcgaag ccactacagt ggtgctgagg
     6481 atgtactgcg ggaagcgttt gcacaaaaag aaagcctctt gcttcaggcg gtgcagaatg
     6541 cccggaaaga agccaagcag gagctggaag aacagaggga ggcactgaat cagcgcgaat
     6601 ccatcctgca gaaaaaggag ctggcgctgg aaacaaccct ggagaaggcc cggaaatatg
     6661 tggccgggga gcggcataac accgtgctgg cccgtctgga agcagagaaa gcagaggaaa
     6721 gacggagaaa tgcctgcgcg acggcggaac ggcgcagacg gaaactggaa aaactccaga
     6781 ccactgacgg aacagcaggg caaaaacact gaggcatttc aggactgcca gaaacgccac
     6841 caggggcggt tcgtgttctg ccgcttctct tcccggttct ccagcaacaa catcgcctgt
     6901 ttcaggctgt cgatatgtga atcttttgag gcaagaaggg tgttcaggtg ttcaatttcc
     6961 tgtttcagaa tgtaaatggc ctgttcatta tggtcagaag tgactgtgtc acgctgtcca
     7021 ctgacctgtt cttcctcctg ttcaacggat acgggcgcaa aggcaccaaa aaaccgcaaa
     7081 agctctgaag tatcgagcat tttcgcattc cgttcacccc gacaggtgga cagttcaccg
     7141 gctttaatta acctgtgcag ggttgtcctg ctcttacccg tcaggcgtgc tgcctcactg
     7201 atcgacacgt tcgccatcag tcaccctcct gtagtgcatc cagtcggagg gacagtgtac
     7261 ggaaaagatg ctccgcatca tcgtggagtt tctcacaaag gtcagccccc gcatcgagat
     7321 caagctcgtt aagcttctcc agcaggagca gggactgatt ctggatgaat ttagccatgt
     7381 tcagggcagt tttctgttgt ttcgtcataa ggggcctcag ggcgtcagtg ctcagtgtaa
     7441 aaccggtctc ttttccgctt cctcgctcac tcagtcgcta cgctcctgcc gttcggctgc
     7501 ggcgagcgga cgggacacac agaagagtca tcgctatcac caggaggaga agagcctgtg
     7561 gaaaaggcca cggcgttgcc gtttttccat aggctccgcc cccctgacaa gcatcacgaa
     7621 atctgacgct caaatcagcg gtggcgaaac ccgacaggac tttaaagata ccaggcgttt
     7681 ccccctggtg gctccctcgt gcgctctcct gttcctgcct ttcggtttac cggtgtcatt
     7741 ccgctgttat ggccgcgttt atctcattcc acgcctgaca ctcagttccg ggaaggcagt
     7801 tcgctccaag ctggactgt
//
