LOCUS       NZ_CP033860            11600 bp    DNA     circular CON 05-JAN-2024
DEFINITION  Acinetobacter sp. FDAARGOS_493 plasmid unnamed3, complete sequence.
ACCESSION   NZ_CP033860
VERSION     NZ_CP033860.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN10163169
            Assembly: GCF_003812245.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter sp. FDAARGOS_493
  ORGANISM  Acinetobacter sp. FDAARGOS_493
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter.
REFERENCE   1  (bases 1 to 11600)
  AUTHORS   Minogue,T., Wolcott,M., Wasieloski,L., Aguilar,W., Moore,D.,
            Jaissle,J., Tallon,L.J., Sadzewicz,L., Zhao,X., Vavikolanu,K.,
            Mehta,A., Aluvathingal,J., Nadendla,S., Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11600)
  AUTHORS   Minogue,T., Wolcott,M., Wasieloski,L., Aguilar,W., Moore,D.,
            Jaissle,J., Tallon,L.J., Sadzewicz,L., Zhao,X., Vavikolanu,K.,
            Mehta,A., Aluvathingal,J., Nadendla,S., Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2018) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, Silver
            Spring, MD 20993-0002, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SMRT v. 2.3.0, HGAP v. 3.0, SPAdes v. 3.6.0
            Genome Coverage                   :: 1667.02737x
            Sequencing Technology             :: PacBio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/04/2024 21:26:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,897
            CDSs (total)                      :: 3,802
            Genes (coding)                    :: 3,716
            CDSs (with protein)               :: 3,716
            Genes (RNA)                       :: 95
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 34 of 86
            Pseudo Genes (incomplete)         :: 49 of 86
            Pseudo Genes (internal stop)      :: 17 of 86
            Pseudo Genes (multiple problems)  :: 11 of 86
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP033860.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11600
                     /organism="Acinetobacter sp. FDAARGOS_493"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_493"
                     /isolation_source="UCC isolate"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_493"
                     /db_xref="taxon:2420300"
                     /plasmid="unnamed3"
                     /collected_by="USAMRIID"
     gene            complement(join(11494..11600,1..>28))
                     /locus_tag="EGY13_RS19545"
                     /old_locus_tag="EGY13_19555"
                     /pseudo=""
     CDS             complement(join(11494..11600,1..>28))
                     /locus_tag="EGY13_RS19545"
                     /old_locus_tag="EGY13_19555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140617.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /name="transcriptional regulator"
     gene            96..1261
                     /locus_tag="EGY13_RS19550"
                     /old_locus_tag="EGY13_19560"
                     /pseudo=""
     CDS             96..1261
                     /locus_tag="EGY13_RS19550"
                     /old_locus_tag="EGY13_19560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611942.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISAba22 family transposase"
                     /name="IS3-like element ISAba22 family transposase"
     gene            complement(1618..2079)
                     /locus_tag="EGY13_RS19555"
                     /old_locus_tag="EGY13_19565"
     CDS             complement(1618..2079)
                     /locus_tag="EGY13_RS19555"
                     /old_locus_tag="EGY13_19565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004781077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DIP1984 family protein"
                     /protein_id="WP_000761345.1"
                     /translation="MKLAEALLLRSDQQKKIISLKQRINANVLVQDGDQPSEDPNELLK
                     QVFSLIQEFQKLSYAIHETNALTKLNDGRSLLALLTLRDEFVEQHKTLTAAISNTSRES
                     DRYSTREIKWHKVIPVSSLQKQADDISLKLRDLNVLIQSNNWKIDLIEA"
                     /name="DIP1984 family protein"
     gene            2387..2590
                     /locus_tag="EGY13_RS19560"
                     /old_locus_tag="EGY13_19570"
     CDS             2387..2590
                     /locus_tag="EGY13_RS19560"
                     /old_locus_tag="EGY13_19570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000481666.1"
                     /translation="MFNFKIFNKVSTEVLTIKNDLQLNSEVQLINKYKTSTSEEYRKAI
                     VLIFKERGYTWLEMGQLFERSI"
                     /name="hypothetical protein"
     gene            complement(2775..2918)
                     /locus_tag="EGY13_RS19750"
     CDS             complement(2775..2918)
                     /locus_tag="EGY13_RS19750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001125246.1"
                     /translation="MPKLKDIALGIIVAPLLIPIMLIASYQDKKALKKELDECQKEKDQ
                     AS"
                     /name="hypothetical protein"
     gene            complement(2938..3513)
                     /locus_tag="EGY13_RS19565"
                     /old_locus_tag="EGY13_19575"
     CDS             complement(2938..3513)
                     /locus_tag="EGY13_RS19565"
                     /old_locus_tag="EGY13_19575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005102333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_001096617.1"
                     /translation="MAKLSLSEVSKKFHVDRSTIYRAVRNGRLSRSSDGQFDLAEVIRC
                     FGEPEQTSQKIESSKQEGDESTKKLIAHLENEVKKYQEREERLMQQIDRMQTLIELKSV
                     APATAAPHQDATACDTKMPQYATTQQDTDNKKNNELNIAENVAVPQQETTAYHTQTLQH
                     ATLQSVAVPQHKKRGLFGRVLNAVFDND"
                     /name="plasmid replication DNA-binding protein"
     gene            complement(3506..4456)
                     /gene="repM"
                     /locus_tag="EGY13_RS19570"
                     /old_locus_tag="EGY13_19580"
     CDS             complement(3506..4456)
                     /gene="repM"
                     /locus_tag="EGY13_RS19570"
                     /old_locus_tag="EGY13_19580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786597.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_001208779.1"
                     /translation="MRELVVKDNALINASYNLDLVEQRLILLAIVEARESGKGINANDP
                     LTVHAESYINQFGVHRNTAYQALKDACNDLFARQFSYQKINERGNIENYRSRWVSEIGY
                     VDNEAVVKLIFAPAIVPLITRLEEHFTKYELQQVSNLSSAYAVRLYELLIAWRSTGSTP
                     IIELSDFRQRIGVLDTEYKRMERFKTSVLELAIKQINEHTDITVKYEQHKRGRSISGFS
                     FNFKQKKKDSTSIERDPNTLKLSSKMTDAQRHMFANKLSELPEMGRYSQGTESYPQFAV
                     RIAEMLQDPEKIKELSPYLKKVGYMPSNKKDTVNG"
                     /name="repM"
     gene            5142..5360
                     /locus_tag="EGY13_RS20050"
                     /old_locus_tag="EGY13_19590"
     CDS             5142..5360
                     /locus_tag="EGY13_RS20050"
                     /old_locus_tag="EGY13_19590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002031357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002031345.1"
                     /translation="MNGQKIDFLRFFKQGNKYFFPCKTKHRPYVTLANFPIQDPAHAGI
                     FLSHTTHLTMTTFKSLCCKGLGNYFVC"
                     /name="hypothetical protein"
     gene            5943..6134
                     /locus_tag="EGY13_RS19975"
                     /old_locus_tag="EGY13_19595"
     CDS             5943..6134
                     /locus_tag="EGY13_RS19975"
                     /old_locus_tag="EGY13_19595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004641936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000106168.1"
                     /translation="MSTNITTTTNTIMFPSACTLCQSRKNVQLFAGLLLCEQCQENIRL
                     TNLGMFSANDETEQKAHD"
                     /name="hypothetical protein"
     gene            6322..6510
                     /locus_tag="EGY13_RS19590"
                     /old_locus_tag="EGY13_19600"
     CDS             6322..6510
                     /locus_tag="EGY13_RS19590"
                     /old_locus_tag="EGY13_19600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004282089.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="WP_000670253.1"
                     /translation="MKFLIALLFPWLSFFIIGRPFAAMICLFLQVTLLGWLPAAIWAVH
                     ALLQDRADQEAKAALYS"
                     /name="YqaE/Pmp3 family membrane protein"
     gene            6925..7365
                     /locus_tag="EGY13_RS19595"
                     /old_locus_tag="EGY13_19605"
     CDS             6925..7365
                     /locus_tag="EGY13_RS19595"
                     /old_locus_tag="EGY13_19605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000368857.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA];
                     GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="WP_000368857.1"
                     /translation="MDNIYPSPQQKTLQKEIAHSQMHHNRFVRLIFMILGAVFFILGLI
                     GIVLPVLPTTPFILLTATCWAKGSIKFNNWLLNHKIFGKMVIDWQQHRAIPRKAKYLAW
                     SMMALSCGMLFYRFSDNWQWLAWATCVICITVAIWMSRLPDA"
                     /name="YbaN family protein"
     gene            7500..10073
                     /locus_tag="EGY13_RS19600"
                     /old_locus_tag="EGY13_19610"
     CDS             7500..10073
                     /locus_tag="EGY13_RS19600"
                     /old_locus_tag="EGY13_19610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002018805.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent hemoglobin/transferrin/lactoferrin
                     family receptor"
                     /protein_id="WP_000957079.1"
                     /translation="MLTMVNAAFFNDNVDVKRVVVNFYYRKLGMLKGSAYRKSIHIPIL
                     EIRSKTLLSILVGTLMYQSALADIQQKEEDLPTLYMDKITVYAKKNPPINVGDDIKTAK
                     TISDNLIDSNKDLVRYNPDISVSDAGRYGSNGYAIRGVDGNRVALNLDGVSLPDKQVNE
                     IFSAYGYMFEGRLSPDVEILSEVAFEVGSDSFNSGSGAMGGAVSFKTKDPEDLIKPNRQ
                     LGGYAKTGYSSSNEEISESFGLAGRNDKLEAIINYVHRRGHETKNHRMIGFDKEKLNPA
                     YDFINDPDYAYPRTGYRSNTAAILPDPLTYETEAALAKLYVHLNEESRLGFHATKQKTD
                     TLSNNFSRKTFSPRIGKDNVELDSYGISHRYIPSDSELIDQIDTNLNYQKAKAAANTET
                     YSQDWGTDNYRLDGLKHRPQIDKTLQLSIAASSFPVQTEKLGEHIFSVNTKYAKKDHKL
                     ILEESFIPASGTAESWYDFLGPSVKQDVFNIAVQDKINLTDKLDTVLGIRYDHYNYKPY
                     MDEINKKGIEKSSKYYPVRIAYENGEFDHNKTMSNIGGMFSVNYELTPDLQAQYQISTG
                     FMAPATSQMYSAFEMMGNSLTPNFHLKPEKSLNHEISLRGEYKDFSFKATGFYTKYSDF
                     ISLLHYQEEQERCYEDYSSGKPVCSPHTVNMIGAKNIGNAKTYGIRLGGMWDIAQLVDT
                     EGDIKVFANLNYAKDESDKGTNLLATQPLNAMLGIGYQSSKKDWQLSGNLRYLGRKHPN
                     DAKVVNLESNGFNSPYQEYIAPYKHIDRSKSAYVYDVYGSKKIGSQLKLSAGIFNLFNT
                     NYVPWDSLRSLAELNVNSMVDDKGVGIERFTAPGRNYKVGLTYEF"
                     /name="TonB-dependent hemoglobin/transferrin/lactoferrin
                     family receptor"
     gene            10189..10764
                     /locus_tag="EGY13_RS19605"
                     /old_locus_tag="EGY13_19615"
     CDS             10189..10764
                     /locus_tag="EGY13_RS19605"
                     /old_locus_tag="EGY13_19615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052939.1"
                     /GO_function="GO:0004392 - heme oxygenase (decyclizing)
                     activity [Evidence IEA]"
                     /GO_process="GO:0006788 - heme oxidation [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="WP_001052940.1"
                     /translation="MNLPLSEALKKYSRQTHDSVDALVLSMHPFASVENYSKFLQAQDI
                     FQKTLKPIYDDLDWNYKIEGLSQLSRLKQISKDMQDLNVNPVSMVEEQPNPSGFEAIGW
                     LYCSEGSNLGAAILCKEAQKLNLSDSYGARHLAAHEEGRMRHWRKFKEQLDGLELTSEQ
                     RQQALQGAEDAFAFFKRVIRQVNPRGTK"
                     /name="biliverdin-producing heme oxygenase"
     gene            10847..11479
                     /locus_tag="EGY13_RS19610"
                     /old_locus_tag="EGY13_19620"
     CDS             10847..11479
                     /locus_tag="EGY13_RS19610"
                     /old_locus_tag="EGY13_19620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011954795.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_000470633.1"
                     /translation="MFIRAYLRASTKEQDANRAKDELIAFAREHGHKIAAFYTENESGA
                     TLERPQLMQLIDDASEGDVILVEQIDRLARLNQTDWDTLKRKLSAKRLSVVSKELPTSY
                     MALQQGNGSEFMDSVLRAINDMLLDMLAAIARKDYEDRRNRQMQGIARAKAQGKYKGRG
                     KDMEKRKIIASLLKSGHSYSDIQQTVKCSRQLIAEVSKASKPISSSI"
                     /name="recombinase family protein"
     Typing          complement(3507..4456)
                     /name="rep_cluster_1172"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.767"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          408..1258
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="85.211"
                     /coverage="100.0"
                     /evalue="1.07e-162"
ORIGIN
        1 tgccacggtt ttctatagca gataatgagt gatgtgtgct gaaaaaacag gaggttttaa
       61 cttggtaaac taaacacact catcaggagc ttaccatgag caagaaacac aagacttaca
      121 ccacagaatt taaagctgaa gccatcaaat taattgaagc caatcaaggc aatgtctcgg
      181 aaacagctag acaacttagc atttcaatgc aaactctttc aaattggaat accaaagcaa
      241 aggctggaac tttagcaggt acaaaacagt attcacctga tctaaacgct ctactcgaag
      301 aaaataaaaa actcaaacaa cagctcaaaa tagctgaaat ggaacgtgaa tttttaaaaa
      361 aggcagcagc gtactttgcc aaagaaagtc agtaaggtac gcctatatga aacaaaaaag
      421 atattctttt ccaattacct taatggctcg attacttcat gtttcagttt catgttttta
      481 tgattggctc aagagaggcg tgagcaaaag aacgattcaa cgaaatcaac agatgatatt
      541 ggtgaaaata gcccatgagg agacaaggca gagctatggt tatattcgat taaccaaata
      601 cttacaagct cagggcataa aaatgagtat gtacgctgta cgtcagataa aagcgctgaa
      661 ccacctgtat tgtaagcgac acaagcgttt taaaaggact acgaatagtg accataatcg
      721 agcgatctat gaaaacctgc tggagcaaca attctcaatg actagaccaa atcaagcatg
      781 gtcaagtgat attacgtaca tatggactgt tgaaggatga ctgtacttag cagcggtaaa
      841 agacctttac acgaagcaag tggttggcta tagcttaaat gagcgcatga caacacagct
      901 tgtttgtaat gcgctaaata tggctattca caatcaaaaa ccaaccaaag aactgattgt
      961 gcattcagac agaggaagtc aatattgcag ccatgaatat cgaaatatac ttgagcaata
     1021 tggttttcaa ggttcaatga gcaagcgcgg agactgttac gataatgcac cgattgaaag
     1081 cttttgggga atactgaaaa atgagttagt gcatcattac aactatcaaa ccagagaaga
     1141 agccaaagca gatattataa aatacattga gttattttat aatcatcgaa gaattcaaaa
     1201 gggtttgggt tttaagacac caaatcaaat ggccgaagac ttttataagt tggctgccta
     1261 gaatctccca agggaaagtc tcctgataat tcagcgtata tcaatctttc agctctttat
     1321 acaacaaagc cgaactgaat taaaaataga aatgtagttg agctgatgct tgctcatttc
     1381 tttattttta gtaattgagg tgaacatact gtcagtacat ggtagttata agtaacgtgt
     1441 aactttgttt gcacaaagat ttgagttatg ccaagtgatg gattgatttg tacttaaaat
     1501 tttaagctta tccacttgct ttgcaagcca ctacacttta tttacaatcc tatgttgtgg
     1561 aagtaaggga tcttgtactc gcccagtatg ttcaacttga ttatatctaa aggactatta
     1621 ggcttcaatc aaatcaattt tccaattatt agactgaatc aacacgttta aatcacgtaa
     1681 tttaagacta atatcatcag cttgtttttg taatgatgaa actggaataa ctttatgcca
     1741 ctttatttcc cgcgtactat aacgatcaga ttcacgagat gtattactta ttgctgcagt
     1801 tagggttttg tgctgttcta caaattcatc acgcaaagtc aataaagcaa gtagagaacg
     1861 gccatcattt aattttgtta aagcattagt ttcatgaatt gcataactta atttttgaaa
     1921 ttcttgaatt aaagaaaata cttgtttcaa tagttcattt ggatcttcag atggttgatc
     1981 accatcttgt accaatacat ttgcattgat acgttgtttt agtgaaataa tttttttttg
     2041 ttgatcactt cgaagtaaga gagcttctgc taatttcatt tttcacctat taaccaatta
     2101 gaattaagtt ttaagcagct aagtaatttc acaaatacta tatcctcatt ctttaaagat
     2161 caaaaaaatt gctttttaat ctaactaaaa ggtgtctaca aaatcggtgg ttattcaacc
     2221 attatgttaa gtgaagaata atttcgtcga atattcaggc tctcaattct gcaaaacttg
     2281 cagaatcttg tcgaatttgt aatccttacg cccaagggtg cctagctcag ctaaggcagg
     2341 tgtttgttgt agtcgggccg ctacaatatt aatgaagagc gcctgtatgt ttaatttcaa
     2401 gatttttaat aaagtctcta cggaagtgtt gacgataaaa aatgacctcc agcttaattc
     2461 tgaggtgcaa ttaatcaata aatataaaac ttctacttct gaagagtata gaaaagctat
     2521 tgttttaatc tttaaagaac gtggttacac ctggctagaa atggggcagc tattcgaaag
     2581 gtcaatttaa actttttcta aaattaacat aatacacctt atacgaaatg ctgtataatt
     2641 atccttataa ttcaacgctg tagtttctag ttgatagccc atcttcccca aggtgggctt
     2701 tttttatgac ttttcacgtt ccacgcctat tatttcatca atgttccaca cttttgatta
     2761 gctcaattga ataatcagga tgcctggtct ttttcttttt ggcattcatc cagctctttc
     2821 ttcagtgcct ttttatcctg gtacgatgca atcagcatga tcgggatcag cagcggagcc
     2881 acaataattc ccagggcaat gtctttcagt ttcggcatga tctttcaccc ttattgctca
     2941 gtcattatca aagacggcat tcagcacacg gccaaataaa ccacgttttt tgtgttgtgg
     3001 cactgccaca ctttgcaacg tggcatgctg tagcgtttgg gtgtggtatg ccgtagtttc
     3061 ttgctgtggc actgctacat tttctgcaat atttaattca ttattctttt tattatcagt
     3121 gtcttgttgt gttgtcgcat actgtggcat cttggtgtcg catgccgtag catcttggtg
     3181 tggtgctgct gtggcaggtg caacactttt cagctcaatg agtgtttgca tacggtcaat
     3241 ttgttgcatt aaccgttctt cacgttcttg gtattttttg acttcatttt ctaaatgggc
     3301 aataagtttt tttgtagatt catcaccttc ttgcttagat gattcaattt tttgagatgt
     3361 ttgctcaggt tctccaaagc atcgtatgac ctctgctaga tcgaattgtc catcactgga
     3421 gcgtgataaa cgtccattac gtacagctct gtaaatggtt gatctatcca catgaaattt
     3481 tttagatact tcacttagtg ataacttagc catttacggt gtccttttta tttgatggca
     3541 tgtatcccac tttttttaga tatggggata gttctttgat tttttcaggg tcttgtagca
     3601 tctcagcaat acgaacagca aattgaggat agctttctgt tccttgtgaa tagcgaccca
     3661 tttcagggag ttctgaaagt ttatttgcaa acatatgccg ttgagcatcg gtcatctttg
     3721 aagaaagttt caaagtattc ggatctcttt ctattgatgt gctgtccttc tttttctgtt
     3781 taaagttaaa agaaaatcct gaaattgatc gacctctttt gtgttgttca tacttcactg
     3841 tgatatctgt atgttcgtta atttgtttaa tagcgagctc aagtacacta gttttaaagc
     3901 gttccatacg cttgtactca gtatcgagta cgccaattct ttgacggaaa tcacttagct
     3961 ctataattgg agtagagccc gtacttctcc aagcaattaa taactcatat aaacgaacag
     4021 cataagcact actgagatta ctaacttgct gcaattcata tttagtaaaa tgctcttcta
     4081 agcgggtaat taatggaact atggcagggg caaaaataag cttaaccact gcttcattat
     4141 ctacatatcc aatttcacta acccaacggg atctatagtt ctcaatattc cctctttcat
     4201 ttattttttg atagctgaat tgtcttgcaa acaaatcatt acaggcatct tttaatgcct
     4261 gataagcggt attgcgatgt acgccaaatt gattgatata gctttctgca tggacagtta
     4321 agggatcatt agcattaatc cctttcccac tttctcttgc ctcaacaata gctaataaaa
     4381 ttaaacgttg ttctactaaa tctaagttat aacttgcatt aattaaagca ttgtctttta
     4441 caactaattc tctcatgaag caccagtatt atttattatg ctatgacacc ttaattaatt
     4501 ttagtgctat aagcaaggta aacggtgtca tattgcaagg taaacggtgt catattgcaa
     4561 ggtaaacggt gtcatattgc aaggtaaacg gtgtcatatg aagtctagga gccttcattt
     4621 acaaggtttt tagcttacct aaaagcattt aaaaacttta aaataattaa aagcccaagg
     4681 aaggaaaaat tgcccttggt tttcgcttcg ctcaaactct attgaacttc gctttcgctc
     4741 agttcgttgg ggcaattttt tggttaatac ttgcgtgact ttaagaaaaa gcaaaagcaa
     4801 ctcggaattc gcttcgctca taaaactttt ttactcgcta cgctcgatcc aagagcaaat
     4861 ttctgcatga aacttacgct ttcttaggct aaagaagaca aaaaaatgtt tttcagatgg
     4921 tctaggcgca aattttgata gttcgctcgt agacactcgc tctatgcatc tatctaaagt
     4981 caaaatacct ctttatttcg ctctcatttg cgttttttga gctgttttgt gtttttcact
     5041 atgagatcta tgttttctag gtaaaggctc ttaaatcgca tttgaacgcg ttctatgaag
     5101 cttttcataa ttaaagtttt gggttgaaat ttttaacctg gatgaatgga caaaaaattg
     5161 attttttacg tttttttaaa caaggaaata aatacttttt tccatgcaaa accaagcacc
     5221 ggccctacgt cactttagcc aattttccaa tccaagatcc ggcacatgcc gggatttttt
     5281 tgtcacacac cacgcatcta acgatgacaa ccttcaaatc cttatgctgt aagggtttgg
     5341 gtaattattt tgtgtgttag aaagctgcgc agaaaaaagc ccatttttta aaataagtta
     5401 ttgattttat ttattaattt gctgcgctct tatctgcgct caaaaaacca ataaaaatgc
     5461 tctaagtaat tgttatttaa tggaaaatag ggtgcgcacc tatctgcgct cgaaaaatgg
     5521 aaaaaaacag cataagttat taatttatat agataaatac cctgcgcagt tattgaaaat
     5581 caagtttgcg caggtaaaag ctataaaaac agcataagta attgaaaatt aattaaaata
     5641 atctgcgcac ttttcgataa gcaacaaaat tgtcaattcg gtcaatttaa caaattataa
     5701 atgacctatt ttttaggaat ttaaacctgt aaaaaccact cagttgatag gttttaagtg
     5761 aggttttaga acctaaattg aattcttatt ttaggcgcta aaagtagtta aaaattgaga
     5821 gtattcaata tcacagagtt ctttatctat tttctacaaa agttctcaaa atggatattc
     5881 ttcaatattc aagcgatgaa gtttaagaca agatattgag aactcttatt ctaggaattg
     5941 cgatgagcac taatatcacc accacgacaa atacaattat gtttccgagc gcttgtactc
     6001 tttgccaatc aagaaaaaat gtacagcttt ttgcaggact gttgctatgc gaacagtgcc
     6061 aggaaaatat caggctcacg aatttgggta tgttctcggc caatgatgag actgaacaaa
     6121 aagctcatga ctaattttga ctagataatt tttagtttgc atttagtgct gtcaaataac
     6181 gagaatttat gaataaagtc aaagtgcgcg gtcaattaca taaataagta aatcttgaat
     6241 agacggacag gcctataggt tgtttacttc tgcctcttat tagttgttaa ggcttttgaa
     6301 acttcctaag aggaaatgaa tatgaaattt ctgatagctt tgctctttcc ttggttgtct
     6361 ttctttataa taggtcgacc ttttgcagca atgatatgtt tgtttcttca agttaccctt
     6421 ttaggctggc tcccagctgc gatctgggct gtgcatgctt tattacaaga tcgtgcagac
     6481 caggaagcaa aagcagcttt atattcctaa actctctcct aagcattctt gaaattgcta
     6541 ctgtcaaagc tcattgaata atgagctttg cttatttcgc ataagagatt ttatgttaaa
     6601 taatggtttt tatatgatct agtatttcta aatataagtg caattttact aaggttgctt
     6661 agatgctatg gaatctaaat atctaaattg ttcatccccc tttagtatag atcaagcagg
     6721 gctaaagcat agctggtaca agaggtgttc gtgatgagga ctggccacag aatatcaatt
     6781 aattctaagc ttttcttgtt aatagtaatg attatcactt ataatgcaaa gttaggaatt
     6841 gttaatttgt ctatatttta gctagataat taaggattta ttgaataatt tgtatttacc
     6901 gcatgattat cgagctgctt tctcatggac aacatatatc cctctcccca gcaaaagacg
     6961 ttacagaaag aaatagccca tagtcagatg caccataacc gtttcgttcg cctaatattt
     7021 atgattctag gcgctgtttt ttttatacta ggtttaatcg gaattgttct tccagtccta
     7081 cccaccaccc cctttatttt gcttactgcc acctgttggg ccaaaggttc cattaaattt
     7141 aataactggc ttcttaatca taaaatattt ggcaaaatgg tcattgactg gcagcagcat
     7201 cgtgcaattc cgcgtaaagc taaatatttg gcctggtcaa tgatggcgct ttcttgcggt
     7261 atgttgttct accgattttc tgataattgg caatggcttg cttgggctac atgtgtgata
     7321 tgtatcactg ttgcgatatg gatgtctcga ttacccgatg cctaactcct attattatca
     7381 caagatgtct ctttcactcc ctactttata aaaagcaaaa ccctctcaga ttacttattc
     7441 tatttataat aatgattatc atttacgttg taatcaaaat gttttatagt gtaagcacaa
     7501 tgttaacaat ggttaatgct gctttcttta acgacaatgt agatgtgaag agagtggtag
     7561 ttaattttta ctacaggaaa ttggggatgc tcaagggttc agcttataga aagtcgatac
     7621 atatacctat tctcgaaatt aggtctaaaa ccctattaag tattcttgtt gggactttaa
     7681 tgtatcaatc tgccttagcg gatattcagc agaaagaaga agatctaccg acattataca
     7741 tggacaaaat cactgtttat gctaaaaaaa atccccctat aaacgtaggg gacgacatca
     7801 aaacagccaa aaccatttca gataacttga ttgatagtaa taaagactta gttcgttata
     7861 accctgatat aagtgtgtca gatgcaggtc gatatggtag taatggctac gctatcagag
     7921 gtgtggatgg caatagagta gcgttaaacc tagatggcgt tagcctgcca gataagcaag
     7981 ttaacgagat attttcagcc tatggttaca tgtttgaagg gcggctgtcc cctgatgtgg
     8041 aaattttatc tgaggtagcg tttgaggttg gttctgattc ttttaattca ggcagtggtg
     8101 caatgggagg ggctgttagt ttcaaaacca aagacccaga agatttaata aaacctaatc
     8161 gtcaacttgg cggctatgcc aaaacaggtt atagcagcag caatgaagag atcagcgaat
     8221 cctttggttt ggcaggaagg aacgataagc ttgaagcaat aattaattac gtgcatcgta
     8281 gaggccatga aacgaaaaat catcgtatga taggttttga taaagaaaag cttaatcctg
     8341 catatgactt tatcaatgat ccagactatg cttatccccg cacgggttat agaagcaata
     8401 cagcagcgat attgcctgat cccttgactt acgaaacaga agcggcgtta gcgaaactgt
     8461 atgtccattt gaatgaagag agtaggttag gctttcatgc tacaaagcaa aaaacagata
     8521 cgctatctaa taacttttca agaaagactt tttcaccaag aattggtaaa gacaacgtcg
     8581 aattagacag ctatggcata agtcataggt acatcccttc tgacagtgaa ttaattgacc
     8641 aaatagatac caacctaaac taccaaaagg ctaaagccgc tgcgaataca gaaacctata
     8701 gccaagattg ggggacggat aattacaggc tagatggttt aaagcatcgc cctcaaatag
     8761 ataagacttt gcagctaagt atcgcagcga gcagttttcc tgtacaaact gaaaaactcg
     8821 gtgagcatat tttttcagta aatacaaagt atgcaaaaaa agatcataag cttatcttag
     8881 aggaatcatt tattcctgct tcgggcacag ctgaatcttg gtatgatttc cttggtccat
     8941 cggtgaaaca agacgtattt aatattgctg ttcaggataa aatcaacctc accgataagc
     9001 tagatacagt gcttggcatt cggtatgacc actataatta taagccctat atggatgaga
     9061 ttaataaaaa agggattgaa aaatccagca aatattatcc tgtgcgtatc gcctacgaaa
     9121 acggtgaatt tgatcacaat aaaacaatga gtaacatagg aggaatgttc tccgttaatt
     9181 acgaactaac ccctgatttg caggcacagt atcaaatatc taccggcttt atggcgccag
     9241 cgacaagtca gatgtattct gcatttgaga tgatgggaaa cagtttaacg cctaattttc
     9301 acctaaagcc tgagaaatca cttaaccatg aaataagttt gcgcggtgaa tataaggatt
     9361 ttagctttaa agctactggt ttttatacga aatatagcga ctttattagc ttattacatt
     9421 atcaggaaga acaagaaaga tgctacgaag actattcgag tggtaagcct gtatgttccc
     9481 ctcatactgt caatatgatt ggcgctaaga atatcggcaa tgcaaagacg tatggtatac
     9541 gcttaggtgg tatgtgggat attgctcaat tggttgatac agagggcgat atcaaggtgt
     9601 ttgctaattt aaattatgct aaagacgaaa gcgataaagg aaccaaccta ctggctactc
     9661 agcctttgaa tgcgatgtta ggtatagggt atcaatcctc taaaaaagat tggcagttat
     9721 caggaaacct gaggtattta ggcagaaagc atcccaatga tgcaaaggtt gttaatttag
     9781 aatctaatgg ctttaattca ccttatcagg agtatatagc gccttacaaa catattgata
     9841 ggtctaaatc agcctatgtc tatgatgtct atggcagtaa aaaaataggc tcacaattaa
     9901 aactatcagc aggaatattt aacctcttta ataccaatta tgttccgtgg gacagcctac
     9961 gctcattggc agaattaaac gtcaactcta tggtagatga taaaggcgtt ggtattgaga
    10021 gattcaccgc acctggtaga aattataagg tgggtctaac ttacgagttt tagcgaatca
    10081 gctaaaatca gtagcgcatt gtccaattca ggggggaact tgaacatagc atcataaaac
    10141 acaaccacac tcatcatatc taacaaccac acacttttag gaactattat gaatttaccg
    10201 cttagtgaag cattaaaaaa gtattcccgt caaacacatg atagtgtaga tgcgcttgtt
    10261 ttatctatgc acccgtttgc ctctgttgag aattatagta agtttttgca agcgcaagat
    10321 atctttcaga agactctaaa gcctatctat gatgatcttg attggaacta taaaatcgaa
    10381 ggtctgagtc agctatcacg cttaaagcaa atctctaagg atatgcaaga tcttaatgtt
    10441 aatccagttt caatggtcga ggagcaacct aatccaagtg gttttgaggc tattggctgg
    10501 ctgtactgtt ctgagggctc aaatttaggt gcagcaattt tgtgtaaaga ggcacaaaag
    10561 ctgaatctaa gtgattctta tggagccaga catttggctg cgcatgaaga agggcgaatg
    10621 agacattggc gcaaatttaa agagcagcta gatggtctag aattaacatc agaacagcgg
    10681 caacaagcgt tacaaggggc cgaggatgca ttcgcatttt ttaaacgagt gattcgtcaa
    10741 gtaaacccta gaggaaccaa ataaaaacgc cttataaaat attaatagac cacctaaaat
    10801 agactggtat aatagaacca cttataacag actattttgg gtgtttatgt ttattagagc
    10861 ctaccttcgt gcctcaacca aagagcaaga cgccaatcgt gctaaagatg aacttattgc
    10921 ttttgctaga gagcatggcc ataaaatcgc agcattctat actgagaacg agtcaggagc
    10981 gacgctagaa cgtccacagc tcatgcagct cattgatgat gcctctgagg gcgatgtgat
    11041 cttagttgaa cagatagacc gtttggcacg tttaaatcaa actgattggg ataccttaaa
    11101 gagaaaacta tcagccaaac gtctttctgt agtgtctaaa gagcttccaa cgtcatatat
    11161 ggcattgcaa caaggtaatg gctcggagtt tatggacagt gtactacgcg ccataaatga
    11221 catgctgctg gatatgttag cggctatagc tagaaaggat tatgaggata gacgtaaccg
    11281 tcaaatgcaa ggtattgcac gcgctaaagc tcaaggtaaa tataaagggc gggggaagga
    11341 catggaaaag cgtaaaatta ttgccagtct tctaaaatca ggccacagct actctgatat
    11401 tcaacagact gtgaagtgtt caagacaatt gattgctgag gtttcaaagg catcaaaacc
    11461 aatatcctcc tctatataac ctgacgaata tatttataca gtaaagccaa tatgcttccc
    11521 cgttggcagc tccacatcaa tacgaagctt accacccatg gcttcaacat attttttcat
    11581 ggttgagatt ttaaggtcat
//
