LOCUS       NZ_CP033852             1459 bp    DNA     circular CON 18-JUN-2024
DEFINITION  Escherichia coli strain FDAARGOS_497 plasmid unnamed5, complete
            sequence.
ACCESSION   NZ_CP033852
VERSION     NZ_CP033852.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN10163173
            Assembly: GCF_003812945.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1459)
  AUTHORS   Minogue,T., Wolcott,M., Wasieloski,L., Aguilar,W., Moore,D.,
            Jaissle,J., Tallon,L.J., Sadzewicz,L., Zhao,X., Vavikolanu,K.,
            Mehta,A., Aluvathingal,J., Nadendla,S., Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1459)
  AUTHORS   Minogue,T., Wolcott,M., Wasieloski,L., Aguilar,W., Moore,D.,
            Jaissle,J., Tallon,L.J., Sadzewicz,L., Zhao,X., Vavikolanu,K.,
            Mehta,A., Aluvathingal,J., Nadendla,S., Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2018) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, Silver
            Spring, MD 20993-0002, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SMRT v. 2.3.0, HGAP v. 3.0, SPAdes v. 3.6.0
            Genome Coverage                   :: 2600.73023781818x
            Sequencing Technology             :: PacBio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003812945.1-RS_2024_06_18
            Annotation Date                   :: 06/18/2024 02:29:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,401
            CDSs (total)                      :: 5,282
            Genes (coding)                    :: 5,065
            CDSs (with protein)               :: 5,065
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 92
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 217
            CDSs (without protein)            :: 217
            Pseudo Genes (ambiguous residues) :: 0 of 217
            Pseudo Genes (frameshifted)       :: 83 of 217
            Pseudo Genes (incomplete)         :: 151 of 217
            Pseudo Genes (internal stop)      :: 41 of 217
            Pseudo Genes (multiple problems)  :: 51 of 217
            Pseudo Genes (short protein)      :: 1 of 217
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP033852.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1459
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_497"
                     /isolation_source="UCC isolate"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_497"
                     /db_xref="taxon:562"
                     /plasmid="unnamed5"
                     /collected_by="USAMRIID"
     gene            164..604
                     /locus_tag="EGY17_RS27375"
                     /old_locus_tag="EGY17_27410"
     CDS             164..604
                     /locus_tag="EGY17_RS27375"
                     /old_locus_tag="EGY17_27410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000031238.1"
                     /translation="MSKDVERITQINDQGEIIGGFVAVIRPKQKSAFQRHFTMNQDALR
                     ILAKELTGEQFKVLMLMLADLDYENFIQIAQADIAETLGMQKTNVSRAVRALLDVGVIF
                     EGPKVGRSKTYRLNEQFGWKGTVTNHKKALKNGFSVIQGGRI"
                     /name="hypothetical protein"
     Typing          1214..1294
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="93.902"
                     /coverage="87.0"
                     /evalue="4.46e-26"
     Typing          complement(839..1099)
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.13"
                     /coverage="100.0"
                     /evalue="1.84e-104"
ORIGIN
        1 gcagctagga taaatatcta atggaatcaa tgcgttagac gcctaatata aagatacaga
       61 aaggagaata aaaacattaa taaaatctaa gggttacagt tgacatagtt atcaataatg
      121 ataacactgt tatcattatt gataactcaa atagggttca aagatgagta aggatgtcga
      181 gagaatcact cagataaatg accagggtga gattatcggc ggcttcgttg ccgttatccg
      241 ccctaaacaa aaatcagcat ttcagaggca tttcaccatg aatcaagacg cattacggat
      301 cttggctaag gaattaaccg gggagcaatt caaggtctta atgctgatgc ttgcagatct
      361 ggattacgaa aacttcattc agatcgccca agcagatatt gctgaaactt tagggatgca
      421 gaaaacgaat gttagtcgcg ccgttcgcgc gctactggat gttggggtga tctttgaggg
      481 acctaaagtc ggcagatcaa aaacataccg gctgaatgaa caatttggtt ggaaaggcac
      541 cgtaaccaac cacaaaaagg cactcaaaaa cggctttagt gtcattcagg gcggccggat
      601 ttaaagagcc agcggcaggc ggacaatcca cagaacattg catgcaattt ctagtggatt
      661 gtctatcctg cattgctctt ttgccgcctc aaaatccctt gcgtgttttt gctccccgat
      721 ctccagaacg accgagccgc cacggttccg gcagcgcctt gagcgcaacc ggaaaccgtc
      781 tggggagacc cccagacccc ccatcgctgt aacaccgacg gtctacgacc acctaccaac
      841 cgagtggctg cttgtaccgt gcaaatgggc tttgcagggg gctggcgcgg ttcctggcga
      901 cctgaaagcc cctggctagg ggtatcggcg cgcctgacct cctgaaacgc tctgtaagcg
      961 tttttcgcgc cttccatgcc ctgacgggca tttaaacccc gcctcgtgcg tctgagagct
     1021 tctgagcgcg tttgaagggc tatctgacga ctcatccccg aaagaacaac agcggaatca
     1081 ccgacagcat gcgagaggtg gcaccgctcg ccgcagcccc agcgaccgaa cgttagtgag
     1141 cgagtgggcg aggaagcgga agagaacagg aaaccacatt gagcacgtac gcactgatac
     1201 ggggtgtcgg ggcgaagctc tgaccagatg gcaaacggaa tatcgtcgcg ggtgacggta
     1261 ttacatttgc acatcctgtc ctgatttctg aggcgtttta acggtgaacg gacggcaaaa
     1321 acatgatgga gctgttgaac gtggagagcg agaaatcgga caaaaatcat ggctgacgag
     1381 gcttgcagag gagtaagcca gtatacactc cgctatcgct acgtgactgg gtcagggctt
     1441 cgccccgaca ccccaaaga
//
