LOCUS       NZ_CP033846             8424 bp    DNA     circular CON 08-JAN-2024
DEFINITION  Klebsiella oxytoca strain FDAARGOS_500 plasmid unnamed2, complete
            sequence.
ACCESSION   NZ_CP033846
VERSION     NZ_CP033846.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN10163176
            Assembly: GCF_003812925.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella oxytoca
  ORGANISM  Klebsiella oxytoca
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 8424)
  AUTHORS   Minogue,T., Wolcott,M., Wasieloski,L., Aguilar,W., Moore,D.,
            Jaissle,J., Tallon,L.J., Sadzewicz,L., Zhao,X., Vavikolanu,K.,
            Mehta,A., Aluvathingal,J., Nadendla,S., Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8424)
  AUTHORS   Minogue,T., Wolcott,M., Wasieloski,L., Aguilar,W., Moore,D.,
            Jaissle,J., Tallon,L.J., Sadzewicz,L., Zhao,X., Vavikolanu,K.,
            Mehta,A., Aluvathingal,J., Nadendla,S., Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2018) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, Silver
            Spring, MD 20993-0002, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.2, SPAdes v. 3.6.0
            Genome Coverage                   :: 618.531570169491x
            Sequencing Technology             :: PacBio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/08/2024 03:53:13
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,511
            CDSs (total)                      :: 5,396
            Genes (coding)                    :: 5,326
            CDSs (with protein)               :: 5,326
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 34 of 70
            Pseudo Genes (incomplete)         :: 41 of 70
            Pseudo Genes (internal stop)      :: 17 of 70
            Pseudo Genes (multiple problems)  :: 17 of 70
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP033846.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8424
                     /organism="Klebsiella oxytoca"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_500"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_500"
                     /db_xref="taxon:571"
                     /plasmid="unnamed2"
                     /collected_by="USAMRIID < ATCC < AR Prevot < R. Sohier"
                     /note="Clinical Isolate"
     gene            join(8409..8424,1..515)
                     /locus_tag="EGY21_RS28025"
                     /old_locus_tag="EGY21_27990"
                     /db_xref="GeneID:39459701"
     CDS             join(8409..8424,1..515)
                     /locus_tag="EGY21_RS28025"
                     /old_locus_tag="EGY21_27990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017397202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_002011486.1"
                     /db_xref="GeneID:39459701"
                     /translation="MKTLTVLELSKLYNINRQTIYNNIKKGILSKNSENKIDLAEAIRV
                     FGEPVKKQDVKESVKVDIPNSAEVLLLRQQIDMLKNQLDDAKDRESFYQNQIETMQRLL
                     EAPKPNMTTFTDQELVQPSIVKPDPEPDTAETKHDGLTTPEQRENKRIPVPEHVEQEPE
                     KRGFWSRFFRPYD"
                     /name="plasmid replication DNA-binding protein"
     gene            complement(745..1932)
                     /gene="tet(39)"
                     /locus_tag="EGY21_RS28030"
                     /old_locus_tag="EGY21_27995"
                     /db_xref="GeneID:39459698"
     ARG             complement(749..1932)
                     /name="tet(39)"
                     /gene="tet(39)"
                     /gene_name="tetracycline efflux MFS transporter Tet(39)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            1998..2639
                     /locus_tag="EGY21_RS28035"
                     /old_locus_tag="EGY21_28000"
                     /db_xref="GeneID:39459700"
     CDS             1998..2639
                     /locus_tag="EGY21_RS28035"
                     /old_locus_tag="EGY21_28000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016927662.1"
                     /GO_process="GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="WP_004787586.1"
                     /db_xref="GeneID:39459700"
                     /translation="MSKKDIAPQRLTREIVLRTALDMLNEEGIDSITTRKLAQRLGIKS
                     PTLYWHFKNKSLLMEAMAETIINEHHLVSLPIDGMTWQDWLLANSVSFRRALLAYRDGA
                     RLHARTSPSQGHFNTIEAQVALLSHAGFSPVEAVALLMTLGRFIVGWVLEEQQEEIRSD
                     PPFEADPTIYPLMLQGVNTLQNMNADDIFENGIRMVIIGAERQLDIKMQT"
                     /name="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
     gene            complement(3690..3914)
                     /locus_tag="EGY21_RS28045"
                     /old_locus_tag="EGY21_28010"
                     /db_xref="GeneID:39459708"
     CDS             complement(3690..3914)
                     /locus_tag="EGY21_RS28045"
                     /old_locus_tag="EGY21_28010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004676219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002011480.1"
                     /db_xref="GeneID:39459708"
                     /translation="MPYTEFQRLVGKAGLSIKEFAALLDMKPNSITNYSKQGVVPTHIA
                     VIVALISTMKDEGLDFYPIFEKIKSYSKE"
                     /name="hypothetical protein"
     gene            4068..4313
                     /locus_tag="EGY21_RS28050"
                     /old_locus_tag="EGY21_28015"
                     /db_xref="GeneID:39459705"
     CDS             4068..4313
                     /locus_tag="EGY21_RS28050"
                     /old_locus_tag="EGY21_28015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002011476.1"
                     /db_xref="GeneID:39459705"
                     /translation="MYSYPDSNTEKKIALMIINDFFIQKAHELWIFLQLDQCFNDYEAT
                     VIWTRRYLEEHPEGEYSDIQKAFLSCFPEHFFNFDY"
                     /name="hypothetical protein"
     gene            complement(4567..5340)
                     /locus_tag="EGY21_RS28060"
                     /old_locus_tag="EGY21_28025"
                     /db_xref="GeneID:39459707"
     CDS             complement(4567..5340)
                     /locus_tag="EGY21_RS28060"
                     /old_locus_tag="EGY21_28025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004728463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_031946062.1"
                     /db_xref="GeneID:39459707"
                     /translation="MTQEKAKKRGRPAQLLQVAELHAFVEFLLEKDPRSKLENQVVDAL
                     QAEDFDFEMLSEAQQILVKEVLKPYREHMKLKLLFDELSENPKRTDYEEKFVQLYQGYQ
                     NNELDPTKTNILKTMCTRYLKFKAQKLEYKDLELYLSQIQKKEINKKRTAADHRKYQLG
                     GAVIAAYKELGLNIDTLTPDQVRKAIVGNKRIINKIEKSIIFEQIEANLETFLFSWPIF
                     FKVIDKLAMIEYEGEIVPEYIKAIMSSLSECQYKK"
                     /name="hypothetical protein"
     gene            5647..6816
                     /locus_tag="EGY21_RS28065"
                     /old_locus_tag="EGY21_28030"
                     /db_xref="GeneID:39459702"
     CDS             5647..6816
                     /locus_tag="EGY21_RS28065"
                     /old_locus_tag="EGY21_28030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002011489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_002011489.1"
                     /db_xref="GeneID:39459702"
                     /translation="MASYHFSVKSKNKGYAIQHYLYIARLMQYENVRKKSNEILEYVEP
                     GKCMPSWVKDPIEFWQAADTFERENAKVYMEYEIALPNEFTPEQRKTLIETFLDKHIVP
                     QQYPHSYAIHNVKSRLSGEDQPHCHIMFSLKADDGIERTAEQYFKRYNPKDPSKGGAKK
                     IQLQDGHADYSTFLIYIRKQWENHLNDALAQHCPTVTYTLDGQDITIKNQVSADSYEKY
                     NEIHSTLYLPEPKLGVGQQNATAEYLAQIQKIREHNQKERELEERQKQLDQQHEYFYSA
                     ENHPFSSLEVMYYLNYLVKNQKNKEKLKKDFSEIIKTQEPILQQEGWNIHNMNRKLRDS
                     EHNSTPCFEFDVYSPPKNPLPTQNKPPKPIQSEQPKPKSDDYDYGGPGF"
                     /name="MobA/MobL family protein"
     gene            7413..8342
                     /gene="repM"
                     /locus_tag="EGY21_RS28075"
                     /old_locus_tag="EGY21_28040"
                     /db_xref="GeneID:39459704"
     CDS             7413..8342
                     /gene="repM"
                     /locus_tag="EGY21_RS28075"
                     /old_locus_tag="EGY21_28040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005065049.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_002011482.1"
                     /db_xref="GeneID:39459704"
                     /translation="MSELIVKDNALIQASYTLDTVEQRLILLAIAEARETGHGITENSL
                     LEVHASSYINTFNVEKHTAYTVLRDASKSLFDRYVTYHDINPKTGKDRSFHCRWVDKIG
                     YEPQTGIVFLRFTQDIVPLITRLEENFTKYELEQVSRLTSSYAIRLYELLIQWRSAGKT
                     PIFDLSIFRQQLGVEAHQYKTMSNFKTYVLDFALKQVNELTDITAKYEQHKKGRSISGF
                     SFSFKQKKTNSDKVIKETDTLALFSKMSDKQRHLFANKLSELPEMGRYSEGTESYQQFA
                     VRISEMLQDSQKFQELFPYLQKVGFASA"
                     /name="repM"
     Typing          7414..8329
                     /name="rep_cluster_1226"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.27"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          5648..6813
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttcttgaact ttccaagctc tacaacatca atagacaaac gatttacaat aacataaaga
       61 aaggaatttt aagtaaaaat tctgagaata agattgatct agcggaagca atccgtgttt
      121 ttggggaacc cgtcaaaaaa caggatgtaa aagaatctgt aaaagtagac attccaaatt
      181 cggcagaagt tttactgctt agacagcaga tagacatgtt gaaaaatcag cttgatgatg
      241 ccaaagatcg ggaatctttc tatcaaaacc aaattgaaac gatgcaacgc ctattagaag
      301 ccccaaaacc taatatgact acgtttaccg atcaagaatt agtgcaaccc agcatagtca
      361 aaccagatcc cgaacctgac acagcagaaa ctaaacatga cggattgact actcccgagc
      421 agagggaaaa taagcggatt cccgtgccgg agcatgttga acaggaaccg gagaaaaggg
      481 gcttttggag ccgttttttt agaccctatg attaaccacg attgttaaac tgatatgaac
      541 aaagtcgtga aacattgatc aaagaatagg cgtggaacgt gaaaaatcat aaaaaaagcc
      601 caccttgggg aagatgagct atcaactaga aactacagca ttgatttcta aagataatta
      661 taacgcattt cgtataaggt gtattatgtt aattttagat aaactataga atccattagt
      721 ttttaaacca ataaattaaa tattttatgg aactccagaa tatttattca ggcgatactt
      781 aagcaataag ggtagggtga ttacataaag tattgcagct aatgcccata ccattccagg
      841 agctgatgcc tgagtcataa aatagaggct agtaactaat acaggaccaa ttatagcccc
      901 cagactatta aaactgctta atagcccttg taattcacct tgtctttcat cactaatacc
      961 tctggacatc attgcttgca aagcaggaac ggctactcca ccaagggcaa acaatggtaa
     1021 caacgcaaaa ggcacccatt cttcaacagc aatagataaa agaagtaaac caattgcatc
     1081 gcaagctatt ccacataaca acgtattttt ctctccaaac ctctttacca taggggcgat
     1141 agcaaaagcc tgtgcaatag aatggcatat tccataacta gcaagggaaa cacctgcaat
     1201 aaatatactc cagccataac gatcctgccc atagataacc cataaagttg cggggacttg
     1261 tgaaaccaat gtgataatta aaaagatacc aagcaatgga gccatattag gttgagtgat
     1321 caaataggat aatttcttta atattgattg ctcctgaacc ttctcagtca ttttattgct
     1381 gtgttttgat tcttttaata agactgctgt cattattaaa ttaatcccat tcataaaagc
     1441 agcagcaaag aacggtgcat gtaagttcca ttcacccaaa actccaccta ttaccggacc
     1501 cgctataaac ccaataccaa agacagctcc aaggagacca aacctttttg cacgctcatg
     1561 ggctggagta atatctgaaa cataagctgt tgcaacagcc atgttggcac ctgtaattcc
     1621 cgcaaaaata cgaccaatat atagccacaa tagagaagga gcagcagcca ttaggagata
     1681 atcagccgtt gcaccagcaa ttgaaataat taatacaggt cgacgtccaa atcggtcact
     1741 caacgctcca aggataggtg caaaaatgaa ctgcatcaga gcatacacag ctaataaagc
     1801 accatagtga acaccgcctg cttcagctcc agccaatgac cgtaataatt caggcaagat
     1861 cggcataatt aaaccaatcc caacagcatc cagaaatata gtgattaaaa tcacgctcaa
     1921 tgatttcttc acaaatgcct tctctagcaa tgatagaatt aatattctct cacacactcc
     1981 ctatcagtga tagatttatg agtaaaaaag atattgcacc tcaacgtctg acacgtgaaa
     2041 tagtcctgcg taccgcattg gatatgttga atgaagaagg catagattca ataaccacac
     2101 gcaagctagc tcaacgattg ggtattaaat ctccaactct atattggcat ttcaagaata
     2161 aatccttgtt aatggaagca atggcggaaa ccattataaa tgaacaccat ttagtttctt
     2221 tacccatcga tgggatgaca tggcaagatt ggttgctcgc taattctgta agctttcgtc
     2281 gagcactact agcttaccgt gatggtgctc gtctacatgc aagaactagc cccagtcaag
     2341 gacatttcaa cacgattgag gcccaagttg cgctgttatc tcatgctggg ttttctcctg
     2401 ttgaagcagt tgcattactt atgacattag gaaggtttat tgttgggtgg gtccttgagg
     2461 agcagcaaga agaaataaga tcagacccac cattcgaagc cgatccaact atatatccac
     2521 tcatgctcca aggagtgaat accttacaaa atatgaatgc ggatgacatt tttgaaaatg
     2581 ggattcgtat ggtgattatc ggtgctgaaa gacaacttga tattaaaatg caaacttaac
     2641 tctatactgc tttaagcaac tgttctattt agtaataaag cttttatcct taattattta
     2701 acataataga gcttatacga aacgtatttt aaaatatttt tatttatcaa taatttaaaa
     2761 gctcaccctt gagcacaaaa tgcacaaaaa ttagcttctg taataagtcg gtgagttttc
     2821 aagcaacttt gtaatatttc gccaatagaa ttggttaaaa agtgatcaat ttccgctttt
     2881 ttgcccaatt aacaagactc ttgcatctaa aattatcccc aatttttgtg gataaaattt
     2941 aggctatttt gtacgctttg gctcacatca atctaggccc ttgctgctat gcggcatact
     3001 ggaatttttt tatgatgaag tcatagaaaa caggatgttc caaacaacaa gaataagaaa
     3061 gatcgcacca ggacgtgagg taagacagga gtcatactga gcaacccaaa gaaaccccgg
     3121 catcctgccg gggttttttt atttcttaaa ttacctatca gatttcgatg tatacatctc
     3181 ataatttgat tgtttatatg atttgtatca tgttatataa aataggaaaa aataacttca
     3241 ctataaggaa atcatgaaaa aaaaaattat ctacctagca ggagctatga ccatagtttt
     3301 actagtagcg gtgattaaac atgaaaataa agtaaagagg gatgagcgat tgagatattt
     3361 agattccata atatctaagc aaccttagta aaattgcgtt tatgtttaga catatcagct
     3421 catataaaag ccattattta acataaagtc tcttatgcgc agttcgagtg taagcccaaa
     3481 gtagaaattt ccggtttacc gattgctcga cctaagatct cagaacataa gagtttgatg
     3541 atcactctgg ttctcaaaac tcgaaacttt attgaaaagt tctattgagg tttataagta
     3601 cctcaaaata aaaaaatgcc tgaagttcag gcatttttta cgtcatcaat cgctactaaa
     3661 aagcttacaa ctatcaaaaa aagagagttt tactcttttg aatacgactt aattttttca
     3721 aaaattggat agaaatctaa tccctcatct ttcatcgtag aaatcaacgc aacgattaca
     3781 gcgatatgtg taggaaccac accttgttta ctgtaattcg taatcgaatt gggtttcata
     3841 tccaataagg cagcaaattc cttaattgaa agccctgctt taccgacaag acgttgaaat
     3901 tcagtatatg gcatttaatt aaatttatag aaatagtgtt ttgagattat agcatagggt
     3961 tatatttaca taaaaacaca taatattatg tggtaaacac attattttat gtgttataaa
     4021 tagtttatca acgaaactat ttatcatctc tataggagaa acatactatg tactcatacc
     4081 cggattcaaa cactgaaaag aaaattgctc tcatgattat taatgacttt ttcattcaaa
     4141 aagcccatga actctggatt tttttacaac tcgatcagtg ctttaacgat tatgaagcaa
     4201 cagtcatttg gactcgacgt tatctagagg aacatccaga gggtgaatac agcgacattc
     4261 aaaaagcctt tctttcttgt ttcccagaac acttcttcaa ctttgactat tgagttatga
     4321 atcgtcttta ctcttttgtg gcttgaaacc agaataaacg ctataaaacc ctggcaggat
     4381 accggggttt ttttattgca atattctaca cacctggcaa atgggcaatc atcatcatga
     4441 ttattcggaa tttacccttc ttcttgttta agaaaatgga tatctaaatc caatcaatca
     4501 ctgagaagcc agatatatct agcttttatt attaaaaata acttatttta atttattgat
     4561 ttttaactat tttttatatt gacactctga taaagatgac ataattgctt ttatatactc
     4621 aggaacaatt tcaccttcat actcaatcat tgctaattta tcaatgacct taaaaaatat
     4681 tggccaactg aagagaaacg tttcaagatt cgcctcaatc tgctcaaata ttatagattt
     4741 ttcgatctta tttatgatcc tcttatttcc tacaatggct tttctaactt gatctggtgt
     4801 caaagtatca atattaagac ccaattcttt ataagcagca atgacagcgc cacctaactg
     4861 atattttcga tgatctgctg ctgttctttt tttattgatt tctttttttt gaatctggct
     4921 taagtacagt tctaaatcct tatactccaa tttttgtgct ttaaacttta gataacgagt
     4981 acacatcgtt ttcaaaatat ttgtctttgt tggatctagt tcattgttct gatatccctg
     5041 atacaactgc acaaactttt cttcatagtc tgtacgtttt ggattttcag acaactcatc
     5101 aaacaatagc tttaacttca tgtgctcacg ataaggtttt aagacctctt tcactaagat
     5161 ctgttgagct tcagacaaca tttcaaagtc gaaatcttca gcctgtagtg catcgacaac
     5221 ttgattttct aatttagaac gaggatcttt ttctaataaa aactccacga aagcatgtaa
     5281 ttccgctacc tgaagtaact gagctggtct gcctcttttc tttgcttttt cctgggtcat
     5341 atcatctgcc gccaaatttt cagaactgct accgatttaa atatggtttt accatattta
     5401 aatcggtcag tctgaaaata attatttaaa ataaaaataa aatttcttga aaagaatttt
     5461 tatttttatt ttaaataatt cgcacttgac agtttctcct aatcggagaa actagtacgc
     5521 aatggggcgc ttcccccttg acccccttca accggatttt tcagccatcc aggcattcca
     5581 aatccgggcg gttttcattc cgtttcactg cataaaaacc gatcccagac caacaaaaag
     5641 gtgagcatgg ccagttatca cttcagcgtc aaaagtaaaa ataaaggcta cgccattcag
     5701 cattacctct atattgcccg attgatgcaa tacgaaaacg tccgcaagaa aagtaacgaa
     5761 attttagaat acgttgaacc aggcaaatgc atgccgtcct gggtgaaaga tcccattgag
     5821 ttttggcaag ccgctgatac gtttgaacga gaaaatgcca aagtctacat ggagtatgaa
     5881 attgccctac caaatgaatt cacgccagaa cagcgtaaaa cgctcataga gacgttttta
     5941 gacaaacata tagttccaca gcaatatccg cattcatacg ccatacacaa cgttaaatcg
     6001 cgcctcagtg gcgaagatca gcctcattgt cacatcatgt tctcgctaaa agccgatgac
     6061 ggtattgaac ggacagccga acaatatttc aaacgctata accccaaaga tccaagcaaa
     6121 ggcggtgcaa agaaaatcca gctgcaggat ggccacgcag actattccac ttttttaatc
     6181 tatatccgaa aacaatggga aaaccatctg aatgatgccc ttgcacagca ctgccctacc
     6241 gtcacataca cattggatgg ccaagacatc accatcaaaa atcaggtttc agcagacagc
     6301 tatgaaaaat ataacgagat ccacagcact ttgtatcttc ctgagcctaa gctaggcgtt
     6361 ggtcaacaaa acgcgacagc ggaatatctc gcccagatcc aaaaaattcg agaacacaat
     6421 caaaaagaac gagaactcga agaacgccaa aagcaattag atcaacaaca tgaatatttt
     6481 tatagtgctg aaaaccatcc attttcatca ttggaagtga tgtattacct gaattattta
     6541 gtgaagaatc agaaaaataa ggaaaaactg aaaaaagatt ttagtgaaat tatcaaaacc
     6601 caagagccta tattgcaaca agaaggctgg aacattcata acatgaatcg aaaattgagg
     6661 gattcagagc ataactcaac accatgcttt gagtttgacg tgtattcacc accaaagaat
     6721 ccattaccca cgcagaataa gccccctaaa ccgattcaat ccgaacagcc aaagccaaaa
     6781 tctgatgatt atgattacgg tggtccgggt ttttaaaatt atctggtttt attcaggctt
     6841 tacagagcga gtgtctacga gcgaacagac ccaattaaaa ttttcgccat gctttttctc
     6901 tgattacagg ctcccctttg ctgctaagac tgaggtacag agcatcccga agggtgcgaa
     6961 cagattcaat ttgcttctag gtcgagcgaa gcgagcataa aaaaatgatg agcgaagcga
     7021 attccgagtt gcttttgctt tttctaaaac tcacgaaaat attaaccaaa aaattgcccc
     7081 tcgaatcgag cgaagcgaga ttcaatagag tttgagcgta gcgaaaacca agggcaattt
     7141 ttcatggttt aggcttttaa ttatttttaa tcttttaaat gcttttaagt aagctgtaat
     7201 cctttataca caagggtttc ataactaata aaactaggtt tatcgaccca taaaactagg
     7261 tttatcgacc cataaaacta ggtttatcga cccataaaac taggtttatc gaccttaaaa
     7321 ctaggtttat cgacctcaaa tcaagttgat ttacaaatcc attaaaatag tctattgtac
     7381 aacctagttt aattataaat ttattattag aaatgagtga attaatcgta aaggataatg
     7441 ctttaattca agctagctat accctagata cagttgaaca aaggctgatc ctattagcca
     7501 ttgctgaagc tcgagaaaca ggacacggga taactgaaaa tagcctttta gaagtacatg
     7561 caagtagtta tatcaatacc tttaatgtcg agaaacacac tgcttatacc gtgcttcggg
     7621 atgcatctaa aagcttgttt gatcgctatg tcacgtatca tgacattaat cctaagactg
     7681 gtaaggatcg tagctttcac tgtcgttggg tcgataaaat tggatatgaa cctcaaacag
     7741 ggatcgtttt cctacgtttc acgcaagaca ttgttccact cataactcga ctggaagaaa
     7801 actttactaa atatgaattg gagcaggttt cgagattaac tagctcttat gctattcggt
     7861 tatacgagtt attaattcaa tggagatctg ctggtaaaac gccaattttt gatctatcga
     7921 tctttagaca acaacttggt gtcgaagctc atcaatacaa aacaatgagt aattttaaaa
     7981 cgtatgtttt agattttgcc ctcaagcaag taaatgagtt aactgatata acggctaaat
     8041 atgaacaaca taaaaaagga cgttcaattt ccggtttttc tttcagtttt aagcagaaaa
     8101 aaaccaactc tgacaaagta attaaggaaa cggatacatt agcccttttc tccaaaatga
     8161 gtgataaaca acgtcatctc tttgccaaca aactttctga attacctgag atggggcgat
     8221 attcagaagg caccgaaagc taccagcaat ttgctgtgcg catttcagaa atgcttcaag
     8281 attctcaaaa gttccaagaa ttattccctt atcttcaaaa agttggcttt gcatctgcct
     8341 aaaatttaaa cttgatcatt tgtctattag acagtatatt tttacaagtt taggcatagg
     8401 tttacaggat gaaaaccctt acag
//
