LOCUS       NZ_CP033827             4096 bp    DNA     circular CON 08-JAN-2024
DEFINITION  Klebsiella sp. FDAARGOS_511 plasmid unnamed4, complete sequence.
ACCESSION   NZ_CP033827
VERSION     NZ_CP033827.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN10163205
            Assembly: GCF_003812845.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella sp. FDAARGOS_511
  ORGANISM  Klebsiella sp. FDAARGOS_511
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4096)
  AUTHORS   Campos,J., Goldberg,B., Tallon,L., Sadzewicz,L., Zhao,X.,
            Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S., Geyer,C.,
            Nandy,P., Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4096)
  AUTHORS   Campos,J., Goldberg,B., Tallon,L., Sadzewicz,L., Zhao,X.,
            Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S., Geyer,C.,
            Nandy,P., Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2018) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, Silver
            Spring, MD 20993-0002, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SMRT v. 2.3.0, HGAP v. 3.0, SPAdes v. 3.6.0
            Genome Coverage                   :: 531.332164920635x
            Sequencing Technology             :: PacBio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/08/2024 04:21:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,021
            CDSs (total)                      :: 5,897
            Genes (coding)                    :: 5,769
            CDSs (with protein)               :: 5,769
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 52 of 128
            Pseudo Genes (incomplete)         :: 82 of 128
            Pseudo Genes (internal stop)      :: 26 of 128
            Pseudo Genes (multiple problems)  :: 30 of 128
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP033827.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4096
                     /organism="Klebsiella sp. FDAARGOS_511"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_511"
                     /isolation_source="Endotracheal aspirate"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_511"
                     /db_xref="taxon:2488567"
                     /plasmid="unnamed4"
                     /collection_date="2016-01-10"
                     /collected_by="Children's National Hospital"
     gene            join(4092..4096,1..205)
                     /locus_tag="EGY08_RS30690"
     CDS             join(4092..4096,1..205)
                     /locus_tag="EGY08_RS30690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014538403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_001632067.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFAEWRTMCGLMQRENA
                     ALSERVTDLSTQVLSLSEQLRRLS"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(735..962)
                     /locus_tag="EGY08_RS30595"
                     /old_locus_tag="EGY08_30570"
     CDS             complement(735..962)
                     /locus_tag="EGY08_RS30595"
                     /old_locus_tag="EGY08_30570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_000719360.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPTES
                     DSGLLGYKDANGVKQQINKADVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     gene            complement(984..1217)
                     /locus_tag="EGY08_RS30600"
                     /old_locus_tag="EGY08_30575"
     CDS             complement(984..1217)
                     /locus_tag="EGY08_RS30600"
                     /old_locus_tag="EGY08_30575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461706.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_001036746.1"
                     /translation="MNKKTLLAAMMVALVTFAAAGCSSNQEIKTTDDRTIVTDGKPKVD
                     SDTGLVSYKDAHTGRTEQINRDKITNMSELDN"
                     /name="YgdI/YgdR family lipoprotein"
     gene            1299..1568
                     /locus_tag="EGY08_RS30605"
     CDS             1299..1568
                     /locus_tag="EGY08_RS30605"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_079839079.1"
                     /translation="MNGQEFRLLLTEHGEKEAVLLFGKKSFVAVPDTQNLYGGCYYLYL
                     EDAHKNELLIELNESDLYPGGAIELQHNGGRKIHCEILKVRWSR"
                     /name="hypothetical protein"
     gene            complement(1805..1963)
                     /locus_tag="EGY08_RS31365"
                     /old_locus_tag="EGY08_30580"
     CDS             complement(1805..1963)
                     /locus_tag="EGY08_RS31365"
                     /old_locus_tag="EGY08_30580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032670878.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHEKSLLAAAISNWRYTRDKL
                     RGLEVWA"
                     /name="hypothetical protein"
     gene            2602..2925
                     /locus_tag="EGY08_RS30620"
                     /old_locus_tag="EGY08_30590"
     CDS             2602..2925
                     /locus_tag="EGY08_RS30620"
                     /old_locus_tag="EGY08_30590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001555769.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGSSGLDRVQVVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3134..>3568
                     /locus_tag="EGY08_RS31285"
                     /pseudo=""
     CDS             3134..>3568
                     /locus_tag="EGY08_RS31285"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3603..4088
                     /locus_tag="EGY08_RS31220"
     CDS             3603..4088
                     /locus_tag="EGY08_RS31220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_012782630.1"
                     /translation="MNSLLTLAKDLEQKSKVQQQTTGEMLKAAFSEHEKSVRAELNESE
                     KRISAAIHDHDRMLSSAMSQRTKGMLRMVSQTWLTIVLVSVLLIASSAGILWWQGQQIL
                     DNYTTIREQKSTQAMLSERNSGVQLTTCGEERRRCVRVNPDAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     Typing          2416..2494
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.8899999999999995e-34"
     Typing          complement(1852..2340)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.747"
                     /coverage="94.0"
                     /evalue="7.82e-151"
     Typing          2916..4096
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.731"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ggaactggaa aaacagttgc tgagcgcatt agagcagcta cagcaggact actcgaaaag
       61 gctggacgag tgggagagcg ccttcgcgga atggcggacg atgtgtggtc ttatgcaacg
      121 ggagaacgca gcgctgagcg agcgcgtcac ggacttgagc acgcaggtgc tgagtttaag
      181 cgagcagctg cgccggttgt cgtaaggctg aacgacatcg aggcacaccg ggagcaggag
      241 cgcgcagcgc agcaccagaa agcgctggag ctggaacgat cgcagcgtca gcaggagtat
      301 gacggtccgt cgctgtgata accggataaa acggggcttt cattgaggat ctgcgccgaa
      361 acccggtgcc attcacccgg ctgcatggct atgcagtcgg gtgagattta tcaggcgcag
      421 aatgcacggt ttgcggggtg gtttactgtc ggttttgcag gactgacgcc gcattcgcgc
      481 tgaacccgtc cggagaggtg cgggcaaggg atgttatggt aaaagcatga tgcctggcgg
      541 gtacggaaaa attgtggcga ccgagcctgc agacgcaaaa atccggccca tacttattcg
      601 tctccttcat caggaatgca gtactatcca gccactgcag gtatttggcc cccagcgggg
      661 gctttttttg cctgttgttg gggcagataa agaaaagggc cgcattagcg gcccttttcg
      721 gagagagcga tgcctcaatg cggaatttca gagacttctt taacgtcggc tttattgatc
      781 tgctgtttga caccatttgc gtctttatag cccagcaggc ctgagtctga ttctgtaggc
      841 ttgccgtcgc tgacaattgt acgcccgtcg ttggtgtgga tggcatagct tgtgcgcgtg
      901 cagccggtta gggctgaaag cgcaactgcc gctgcgaaca ctgaaacaaa aatctttttc
      961 aaagccagct ccttttatcc gtatcagttg tctagttcac tcatgttggt aattttgtca
     1021 cggttgatct gttcggttcg gcctgtatgg gcatctttgt aggatactaa tcccgtatca
     1081 ctgtcaactt tgggtttgcc gtctgtaacg atagtgcgat catctgtggt ttttatttct
     1141 tggtttgaag aacatccagc agcggcaaag gtcaccaacg cgaccatcat ggccgccaac
     1201 agcgtttttt tattcataat aggttccctg cttaaaggta aagtggatat ggtcactaac
     1261 catctaagta taggtcgttt tttcatgcga aaagaggatt gaatggacaa gaatttcgat
     1321 tattgctgac agagcatgga gaaaaagagg ctgttttact gttcggaaag aaatcttttg
     1381 ttgccgtgcc agatacgcag aacctttatg gtgggtgtta ttacctttat ttagaggatg
     1441 ctcacaagaa cgagctttta attgagttaa acgaaagtga tttatacccc ggtggcgcaa
     1501 tagaacttca acacaacggc ggtcggaaga ttcattgcga gatactaaaa gtaaggtgga
     1561 gtcgttaact caagtaatgt ttcctaagta tgttgatagg caaacatagt ggtagttagt
     1621 ctctctgagt aaatgatctt cttgtgatcc tgtcgttaca gtcgtaatct cttgctctga
     1681 aaacgaaaaa accacctggg gaggtggttt gatcgaaggt taagtcagtt ggggaactgc
     1741 ttaaccaggt aactgggtat acgagaccgc agaaaccaaa actgttcttt cagtgtagcc
     1801 tctctcaggc ccatacttca agacctctta atttgtctct cgtatacctc cagttactaa
     1861 tggctgctgc cagtagcgat ttttcgtgtc tttccgggtt ggactcaaga cgatagttac
     1921 cggataaggc gcagcagtcg gactgaacgg ggggttcgtg catacagtca atcctggagc
     1981 gaactgcctt cgcggaactg agtgtcaggc gtggaatgag aaaccgcggc cataacagcg
     2041 gaatgacacc ggtaaaccga aaggcaggaa tgcgggggag cacgagggag ccatcagggg
     2101 gaaacgcctg gtatctttaa gccgcatcgg gtttcgccac cactgatttg agcgtcagat
     2161 tctgtgatgc ttgtcagggg ggcggagcct atggaaaacc ggcagcggcg catcctttct
     2221 tcccctcctg cattcacgta tttccccgcc acgtctttat ccgtgagtcc gtaccgctcg
     2281 ccgcagccga acgaccgagc gtagcgagtc agtgagcgag gaagcggaat atccaccggg
     2341 acaggcatca gcctcttacg cacctgagcc tcatctcaca gttttcatga ctgactattt
     2401 catcccgatt cccgtcgtgc caccatagta agccagtata cactccgcta gcgctacgtg
     2461 actggttcag ggctgcgccc cgaaacccgc taaatgccac tgacgcgcct gcggcttgcc
     2521 caacaccgcg ccgaccggga aaagatttcc cgcccgtccc ggcgttatca ggaggccgga
     2581 ttggcagaca aacgcagcaa aatgctcacg atgtgggtaa cagaagatga gcaccgccgt
     2641 ctgctggaac gctgcgacgg taaacagctc gcggcctgga tgcggcagac gtgcctggac
     2701 gagaagcccg cccgggccgg caaacttccc tcgatctcgc cggcgctgct tcgtcagctt
     2761 gccggcatgg ggaacaacct caaccagatt gcccgccagg ttaacgccgg tggcagtagc
     2821 ggtctcgacc gcgtgcaggt cgtcgccgcg ctgatggcca tcgatgccgg actcgagcgg
     2881 ctgcggcatg ccgtactgga aaagggggct gacgatgatc gttaagtttc atccccgggg
     2941 acgcggaggc ggtgccggcc cggttgatta tctgctgggg aaagaccgcc agcgcgacgg
     3001 tgccagcgtt cttcagggga agccggagga agtccgggag cttatcgacg cctcgcccta
     3061 cgccaaaaag tacacctccg gggtgctgtc ctttgccgaa caggacttac cgcccgggca
     3121 gcgtgaaacg ctgatggcga gcttcgagcg ggttctgatg cccggactcg ataaagacca
     3181 gtacagcgtg ctgtgggttg agcaccggga caaggggcgg ctggagctga acttcctgat
     3241 cccgaacacg gagctgctga ccggcaaacg gctccagcca tactatgacc gcgccgaccg
     3301 tcctcgcatt gatgcctggc agaccatcgt gaacggcagt ctggggctgc acgacccgaa
     3361 cgcgccggaa aaccggcggg cactggtcac gccgtccgcg ctgccggaaa cgaagcagga
     3421 agccgctcag gcgattacgc ggggtttact ggccttagcc tcgtccgggg agctgaaaac
     3481 gcgtcaggac gtcactgagg cgctggaaag cgcaggtttt gaggtggttc gcaccacaaa
     3541 aagcagcatc agcattgccg acccggacgg ggggcgaaac atccgactga agggagccat
     3601 ctatgaacag tcttttaacg ctggcgaagg acttagagca gaaatcgaaa gtgcagcagc
     3661 agactaccgg cgagatgctg aaagccgcat tcagcgagca cgagaagtct gtcagagcgg
     3721 aactgaacga aagcgagaag agaatcagcg ccgccatcca cgaccacgac cggatgctgt
     3781 cctcagccat gagccagcgt acgaaaggga tgctgcgcat ggtcagccag acgtggctga
     3841 ccatcgtcct ggtctccgtg ctgctgatag cctcaagtgc gggcattcta tggtggcagg
     3901 ggcagcagat actcgacaat tacacgacca tccgggagca gaagagcacg caggccatgc
     3961 tgtccgagag gaacagcggc gtgcagctca cgacctgcgg agaggaacga cgccgctgcg
     4021 tgagggtgaa cccggacgcg ggacggttcg gagaggattc gagctggatg atactggcgg
     4081 ggaaatagca catgac
//
