LOCUS       NZ_CP033821             1033 bp    DNA     linear   CON 08-JAN-2024
DEFINITION  Klebsiella aerogenes strain FDAARGOS_513 plasmid unnamed4.
ACCESSION   NZ_CP033821
VERSION     NZ_CP033821.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN10163207
            Assembly: GCF_003812185.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella aerogenes
  ORGANISM  Klebsiella aerogenes
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Goldberg,B., Campos,J., Tallon,L., Sadzewicz,L., Zhao,X.,
            Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S., Geyer,C.,
            Nandy,P., Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1033)
  AUTHORS   Goldberg,B., Campos,J., Tallon,L., Sadzewicz,L., Zhao,X.,
            Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S., Geyer,C.,
            Nandy,P., Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2018) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, Silver
            Spring, MD 20993-0002, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.4, SPAdes v. 3.6.0
            Genome Coverage                   :: 846.972375283019x
            Sequencing Technology             :: PacBio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/08/2024 03:50:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,901
            Genes (coding)                    :: 4,828
            CDSs (with protein)               :: 4,828
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 12 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP033821.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="Klebsiella aerogenes"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_513"
                     /isolation_source="Endotracheal aspirate"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_513"
                     /db_xref="taxon:548"
                     /plasmid="unnamed4"
                     /collection_date="2016-01-23"
                     /collected_by="Children's National Hospital"
     gene            complement(<1..581)
                     /locus_tag="EGY11_RS26340"
     CDS             complement(<1..581)
                     /locus_tag="EGY11_RS26340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_process="GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="WP_228711407.1"
                     /translation="MLDANENFVNATGYKRDEVIGKHHRLFCAETLYKSDEYRQFWERL
                     NQGEFFSGQFPRINRQGDPLWFRATYNPVFNSDGQLYKIVKFATDVTADVLRNQREQEA
                     AVHAWDMAVQTRGSAQNGANVIENSILMIDRIAQGMGAVSTDVSRLNNQSESIDGMVET
                     IRKFAMQTRLIALNAAIEAARAGASGRSFAV"
                     /name="methyl-accepting chemotaxis protein"
     gene            complement(574..>1033)
                     /locus_tag="EGY11_RS26345"
     CDS             complement(574..>1033)
                     /locus_tag="EGY11_RS26345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="WP_228711408.1"
                     /translation="KNLFSLNRQRSAISYDAICQNKPVIEFSPEGVIHKASPLFLSTMG
                     YRADEIIGHHHRIFCPPSLVSSPEYSHFWQRLARGESFSGKFLRRAKGNRSVWLEASYI
                     PVTDRRGRVTRVIKIAADISERVHSALEQEAVLNAINRSMAIITFDRA"
                     /name="PAS domain-containing protein"
ORIGIN
        1 accgcaaaac ttcttccgga tgcgcccgcc cttgctgctt caatcgcggc attcaatgca
       61 ataagtcttg tttgcatggc aaacttccgg atggtttcca ccataccgtc aatactttca
      121 gactgattgt tgagccggga gacatcggtg gagacagctc ccatcccctg cgcaattctg
      181 tcaatcataa ggatgctatt ttcaataaca tttgcaccgt tctgcgcact tcctcgtgtc
      241 tgcacggcca tatcccatgc atgcacagca gcctcctgct ctctctggtt tcgcaggaca
      301 tcagccgtga catctgtagc gaatttcaca attttataaa gctgcccgtc actgttaaag
      361 acagggttat aagttgcacg gaaccacaga gggtctccct gacggttaat acgtggaaac
      421 tgaccagaaa aaaactcacc ctgattcagc ctctcccaga actgtcggta ttcatcactt
      481 ttgtaaagcg tctcggcaca aaacagacga tgatgcttac cgatgacttc atcccgttta
      541 tagccagtgg cattgacgaa attttcattt gcatcaagca cgatcgaagg tgattatggc
      601 catagaacgg tttatggcat ttaggactgc ttcctgctca agcgcagaat gcacgcgctc
      661 agaaatatct gcagcaattt taatgactct ggtcacacga ccgcgccggt cagtaacggg
      721 gatataactg gcttcaagcc atacggaacg atttcccttt gcccgccgca ggaacttgcc
      781 actaaagctt tctccccggg caaggcgctg ccagaaatgg ctgtattccg gtgaactcac
      841 cagagagggt gggcagaata tacgatggtg gtgaccgatt atttcatcag ccctgtaccc
      901 catagtggag agaaaaagcg ggctggcttt atgtatcacc ccctccggac tgaattcaat
      961 gacaggctta ttctgacaaa ttgcgtcata cgaaatggct gagcgctggc ggttcaggga
     1021 gaacagattt tta
//
