LOCUS       NZ_CP033820             1055 bp    DNA     linear   CON 08-JAN-2024
DEFINITION  Klebsiella aerogenes strain FDAARGOS_513 plasmid unnamed3.
ACCESSION   NZ_CP033820
VERSION     NZ_CP033820.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN10163207
            Assembly: GCF_003812185.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella aerogenes
  ORGANISM  Klebsiella aerogenes
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Goldberg,B., Campos,J., Tallon,L., Sadzewicz,L., Zhao,X.,
            Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S., Geyer,C.,
            Nandy,P., Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1055)
  AUTHORS   Goldberg,B., Campos,J., Tallon,L., Sadzewicz,L., Zhao,X.,
            Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S., Geyer,C.,
            Nandy,P., Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2018) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, Silver
            Spring, MD 20993-0002, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.4, SPAdes v. 3.6.0
            Genome Coverage                   :: 846.972375283019x
            Sequencing Technology             :: PacBio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/08/2024 03:50:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,901
            Genes (coding)                    :: 4,828
            CDSs (with protein)               :: 4,828
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 12 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP033820.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="Klebsiella aerogenes"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_513"
                     /isolation_source="Endotracheal aspirate"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_513"
                     /db_xref="taxon:548"
                     /plasmid="unnamed3"
                     /collection_date="2016-01-23"
                     /collected_by="Children's National Hospital"
     gene            151..468
                     /locus_tag="EGY11_RS25955"
                     /old_locus_tag="EGY11_25945"
     CDS             151..468
                     /locus_tag="EGY11_RS25955"
                     /old_locus_tag="EGY11_25945"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_228711406.1"
                     /translation="MSEAMEKNRRSVLLTAGRTWLTILMVSALLIGTSGSILWWQGQQI
                     TDNYTHLRQHEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQTEVIPYDGTTWIRLKQ
                     E"
                     /name="hypothetical protein"
     gene            475..690
                     /locus_tag="EGY11_RS25960"
                     /old_locus_tag="EGY11_25950"
     CDS             475..690
                     /locus_tag="EGY11_RS25960"
                     /old_locus_tag="EGY11_25950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137422.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
ORIGIN
        1 aaggagttgg agcatcaatc gaaagggcag tagcagagta ctggcgagat gcttaaagcc
       61 gctttcagtg agctcgagca gtctgtcaga gcggtactga gcgcatgcgc gaggagaatc
      121 agcgacgcca tcctcgccca cgagcagagt atgagcgagg cgatggagaa aaaccggcgg
      181 agcgtgctgc tgacggcagg gaggacatgg cttaccatcc tgatggtttc agcgctgctg
      241 atcggcacga gcgggagcat tctgtggtgg caggggcagc agataaccga caattacacg
      301 catcttcgtc agcatgaaga taccctggcg aaaatgaccg ccaggacgtg gggagtgcgc
      361 tatcaggaga gcagcgacgg ccggaggttt cttatcctgc cgccggggat gcaaaccgaa
      421 gtcatccctt acgacgggac gacatggatc cgcctgaaac aggagtagcg gaagatgaca
      481 gagctggaaa agcagttgct gagcgcatta gagcagctac agcaggacta ctcgcaaagg
      541 ctggacgaat gggagagcgc cttcgcggaa tggcggagca tgtgtgggct tatgcaacgg
      601 gagaacgcgg tgctgaacga gcgcgtgtcg gacttgagca cgcaggtgct gagtttaagc
      661 gagcagctgc gccgcttgtc cgggaactga atgccaccga gttccggcag agggagaatg
      721 agtatcagaa aacgctcgag ctgcagcgcc agccgcagaa aacctacaaa ggaccgacgc
      781 tgggctgaca gatgccggcc cgtgacaaac cccaagaaga agggctctta tctctcccgc
      841 taaataggtt cgtgacaaaa aaggtcaagg cttattcagg agcgggaaat gaaattgctt
      901 aacgttattg aagctgtact taactgtctg tatctggctg cagagctgtg ggagtggctg
      961 gagaagcatt tgccggtttt taaggcattg gcgcggattt tagtgtgact ttaacatcca
     1021 tgcatctggc tgcatgacta tgcaccagtt taata
//
