LOCUS       NZ_CP033819             1303 bp    DNA     linear   CON 08-JAN-2024
DEFINITION  Klebsiella aerogenes strain FDAARGOS_513 plasmid unnamed2.
ACCESSION   NZ_CP033819
VERSION     NZ_CP033819.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN10163207
            Assembly: GCF_003812185.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella aerogenes
  ORGANISM  Klebsiella aerogenes
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 1303)
  AUTHORS   Goldberg,B., Campos,J., Tallon,L., Sadzewicz,L., Zhao,X.,
            Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S., Geyer,C.,
            Nandy,P., Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1303)
  AUTHORS   Goldberg,B., Campos,J., Tallon,L., Sadzewicz,L., Zhao,X.,
            Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S., Geyer,C.,
            Nandy,P., Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2018) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, Silver
            Spring, MD 20993-0002, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.4, SPAdes v. 3.6.0
            Genome Coverage                   :: 846.972375283019x
            Sequencing Technology             :: PacBio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/08/2024 03:50:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,901
            Genes (coding)                    :: 4,828
            CDSs (with protein)               :: 4,828
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 12 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP033819.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..1303
                     /organism="Klebsiella aerogenes"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_513"
                     /isolation_source="Endotracheal aspirate"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_513"
                     /db_xref="taxon:548"
                     /plasmid="unnamed2"
                     /collection_date="2016-01-23"
                     /collected_by="Children's National Hospital"
     gene            complement(<1..377)
                     /locus_tag="EGY11_RS26330"
     CDS             complement(<1..377)
                     /locus_tag="EGY11_RS26330"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_228711405.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQNTGEMLKAAFSEHEKSVKAELNESA
                     KRISAAILDHDRKLSSAMSQRTKGMVRMVSQTWLTIVLVSALLIASSAGILWWQGQQIL
                     DNYTTIREQKSTQAMLSERNSG"
                     /name="MbeB family mobilization protein"
     gene            complement(<412..1116)
                     /locus_tag="EGY11_RS26470"
                     /pseudo=""
     CDS             complement(<412..1116)
                     /locus_tag="EGY11_RS26470"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1055..>1303)
                     /locus_tag="EGY11_RS25950"
                     /old_locus_tag="EGY11_25940"
     CDS             complement(1055..>1303)
                     /locus_tag="EGY11_RS25950"
                     /old_locus_tag="EGY11_25940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_123907226.1"
                     /translation="WMPQTCLDERPARSGKLPSISPALLRQLAGMGNNLNQIARRVNAG
                     GGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTDDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          complement(68..1065)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="84.985"
                     /coverage="86.0"
                     /evalue="0.0"
ORIGIN
        1 ccgctgttcc tctctgacag catggcctgc gtgctcttct gctcccggat ggtcgtgtag
       61 ttgtcgagta tctgctgccc ctgccaccac agaatgcccg cgctcgaggc gatcagcagc
      121 gcggagacca ggacgatggt cagccacgtc tggctgacca tgcgcaccat gcctttcgtg
      181 cgctggctca tggctgagga cagtttccgg tcgtggtcga ggatggcggc gctgattctc
      241 ttcgcgcttt cgttcagttc cgcttttaca gatttctcgt gctcgctgaa tgcggctttc
      301 agcatctcgc cggtattctg ctgctgcgct ttcgatttct gctctaagtc cttcgccagc
      361 gttaaaagac tgttcataaa tggctccctt cagtcggatg tttcgccccc cgtccgggtc
      421 ggcaatgctg atactgcttt tcgtggtgcg taccacctca aaacctgccc gttccagcgc
      481 ctcagtgacg tcctggcggg ttttcggctt cccgcacgaa gcaagggtca gcagtccccg
      541 ggtaatggct tctgcggcct cctgcttcgt tttcggcagc ccggacggcg tgaccagcgc
      601 ccggcggttt tccggtgcgt tcgggtcgtg cagccccagt ctgccgttca cgatggtctg
      661 ccaggcatcg atgcgcggac ggtcggcgcg atcgtagtac ggctggagcc gtctgccggt
      721 cagcagctcg gtattgggga tcagaaagtt cagctccaga cgccccttgt ccgcgtgctc
      781 cacccacaga atgctgtact gatctttatc gagtccgggc atcagaaccc gctcgaagct
      841 ggccatcagt ttttcccgct ggccgggagg cagatcggcc tccgcaaacg acaggacgcc
      901 ggaggtgtat ttcttaacgt aaggcgacgc gtcaatcagc tcccggacct cttccggctt
      961 cccctgcagg acgctggcgc cttcacgctg ccggtcttta cccagcagat agtccaccgg
     1021 accggcgcca ccgccgcgac cccgaggatg aaatttaacg atcatcgtcc gttcctttct
     1081 caagaacggc gtgccgcagc cgctcaagcc cggcatcgat ggccatcagc gccgccacaa
     1141 tctgtaccct gtcatggccg gtgccgccgc cggcgttaac acggcgggct atctggttca
     1201 ggttattgcc catgccggca agctggcgca gcagcgccgg cgagattgag gggagctttc
     1261 cggaacgtgc cggcctttcg tccaggcagg tctggggcat cca
//
