LOCUS       NZ_CP033818             9294 bp    DNA     circular CON 08-JAN-2024
DEFINITION  Klebsiella aerogenes strain FDAARGOS_513 plasmid unnamed1, complete
            sequence.
ACCESSION   NZ_CP033818
VERSION     NZ_CP033818.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN10163207
            Assembly: GCF_003812185.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella aerogenes
  ORGANISM  Klebsiella aerogenes
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9294)
  AUTHORS   Goldberg,B., Campos,J., Tallon,L., Sadzewicz,L., Zhao,X.,
            Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S., Geyer,C.,
            Nandy,P., Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9294)
  AUTHORS   Goldberg,B., Campos,J., Tallon,L., Sadzewicz,L., Zhao,X.,
            Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S., Geyer,C.,
            Nandy,P., Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2018) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, Silver
            Spring, MD 20993-0002, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.4, SPAdes v. 3.6.0
            Genome Coverage                   :: 846.972375283019x
            Sequencing Technology             :: PacBio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/08/2024 03:50:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,025
            CDSs (total)                      :: 4,901
            Genes (coding)                    :: 4,828
            CDSs (with protein)               :: 4,828
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 33 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 12 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP033818.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9294
                     /organism="Klebsiella aerogenes"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_513"
                     /isolation_source="Endotracheal aspirate"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_513"
                     /db_xref="taxon:548"
                     /plasmid="unnamed1"
                     /collection_date="2016-01-23"
                     /collected_by="Children's National Hospital"
     gene            join(8989..9294,1..426)
                     /locus_tag="EGY11_RS25880"
                     /old_locus_tag="EGY11_25870"
     CDS             join(8989..9294,1..426)
                     /locus_tag="EGY11_RS25880"
                     /old_locus_tag="EGY11_25870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_004152551.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="MobC family replication-relaxation protein"
     gene            433..963
                     /locus_tag="EGY11_RS25885"
                     /old_locus_tag="EGY11_25875"
     CDS             433..963
                     /locus_tag="EGY11_RS25885"
                     /old_locus_tag="EGY11_25875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004153058.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(991..1170)
                     /locus_tag="EGY11_RS25890"
                     /old_locus_tag="EGY11_25880"
     CDS             complement(991..1170)
                     /locus_tag="EGY11_RS25890"
                     /old_locus_tag="EGY11_25880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165971.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1239..1616
                     /locus_tag="EGY11_RS25895"
                     /old_locus_tag="EGY11_25885"
     CDS             1239..1616
                     /locus_tag="EGY11_RS25895"
                     /old_locus_tag="EGY11_25885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000312627.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="hypothetical protein"
     gene            complement(1678..1977)
                     /locus_tag="EGY11_RS25900"
                     /old_locus_tag="EGY11_25890"
     CDS             complement(1678..1977)
                     /locus_tag="EGY11_RS25900"
                     /old_locus_tag="EGY11_25890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_001146176.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(1967..2230)
                     /locus_tag="EGY11_RS25905"
                     /old_locus_tag="EGY11_25895"
     CDS             complement(1967..2230)
                     /locus_tag="EGY11_RS25905"
                     /old_locus_tag="EGY11_25895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000072676.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     gene            complement(2536..2955)
                     /locus_tag="EGY11_RS25910"
                     /old_locus_tag="EGY11_25900"
     CDS             complement(2536..2955)
                     /locus_tag="EGY11_RS25910"
                     /old_locus_tag="EGY11_25900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254601.1"
                     /translation="MRRSGSKLCLIAMITATATLTACAPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLQGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            3358..5043
                     /locus_tag="EGY11_RS25920"
                     /old_locus_tag="EGY11_25910"
     CDS             3358..5043
                     /locus_tag="EGY11_RS25920"
                     /old_locus_tag="EGY11_25910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_004178188.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IVTSLPADTVTETPVSSLPLDQATVSVTKRVADIVKDERQHIAVVTGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPAAKAPPKGIIAEKGDSRPAGFTAGGNSREAVIR
                     FPKESGQKPVYVSVTDVLTPAQVKQRLEEEKRRQQAWDAAHPEEGLKREYDKAKAELDA
                     EDKNIATLNSRIASTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQTSLNEMNESLSRDKAALSGAMESRKQKEKKAKDAENKLNE
                     EKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            5053..5310
                     /locus_tag="EGY11_RS25925"
                     /old_locus_tag="EGY11_25915"
     CDS             5053..5310
                     /locus_tag="EGY11_RS25925"
                     /old_locus_tag="EGY11_25915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin immunity family protein"
                     /protein_id="WP_004152553.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin immunity family protein"
     gene            5395..5544
                     /locus_tag="EGY11_RS25930"
                     /old_locus_tag="EGY11_25920"
     CDS             5395..5544
                     /locus_tag="EGY11_RS25930"
                     /old_locus_tag="EGY11_25920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_004152552.1"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            7025..8989
                     /locus_tag="EGY11_RS25940"
                     /old_locus_tag="EGY11_25930"
     CDS             7025..8989
                     /locus_tag="EGY11_RS25940"
                     /old_locus_tag="EGY11_25930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_004178196.1"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEF
                     RPPADGGGPKADDDFAGIY"
                     /name="TraM recognition domain-containing protein"
     BGC             1..9294
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(5648..6226)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.361"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          8990..9294
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="3.64e-50"
ORIGIN
        1 cagccgtcaa gggtgtcggc agcacatctg ccgcaccggc tggccgtgca atccctgcgc
       61 cttgatatgg aagccagagg ggccaccggc tggcgcattc tgcaccgtct gagtcttaaa
      121 ggcatgaaag tgccggacgc cctggcggag ctggacggta aaacggtggc gtttgaggtg
      181 gagcgcaccg tcaagagccg tcgccggtat caggaagtgg tgtccgggta tctgttcaac
      241 cgcaaagcta acggcatcga tgaaatctgg tacatctgcc cggaccgggc gacgcaggtc
      301 agggtgcagc gggcaatcct gtcggtggat gaaatcgtga acccacagac cggcgaggcc
      361 cgcaaaacgg cagagctgga tcgtgaacgg ttgtttgcgt gctttaaatt tatgacaacg
      421 gagtgaggga ttatggcgct gtacatggta tttattgaag acgcggagga ccggaacggc
      481 gagcgctgca tcatgccgac cagcgagcat ctggaagata tggtggtgct cgcctggcag
      541 accggggcgt atgttttctg ttttgtcgac cgggcccagc atctggcgat gatgaaatcc
      601 atcgggatgt atgacgagcg gctcaacagc gacgaaaaag ccacgtactt tttcactggt
      661 aaaaatccgc aatgggaacg gaacaaaccc acaggaatga agctgtgtga ttacctctat
      721 cgcaaggtgg agcggttcgc catcgagaac ggtgtgcagg tggttgatgc cgggaagtgc
      781 tcaccggagc agattaacgc gatgattgac gtggtgagtg agcggcacgg ggaaagcgcg
      841 gtgaacgaga agctgaaagc ggagctgatg cgggaatata acagccgcta ttttaacgtg
      901 ccggtacctg aactgccgga ggacaaacct gcccgccggt ggtggccgtt cggcaaaaaa
      961 taaatgccag caccggccat gccggtgctg ttattcctcc ccgagcttat cccgcatcac
     1021 ctgccagagt gtttccgcat cctcgtgtag ccgctcgcac atgtcggccg gggtatccat
     1081 atccagctca ttcagtttat cgagcaatag cagcgactgc tccttaataa atttagccat
     1141 attgagggct gttttttcct gttttgtcat gattctttcc ttccgggcat ggtgtgcgcc
     1201 tgtcaggtat actgtctcaa aaatagcggg gtgagacaat ggcgtgggtg acggtgagac
     1261 aggcgacgga actgacgggt aaagcccgaa gttccctgta tcgggatatg gcgaaaggcc
     1321 gcgtatcata ccgtacggaa gctgatggcg gccgggtggt ggataccagc gagcttatcc
     1381 gggtgtacgg tgagttaaga caggatgaga ctcacgagcg ggacgagttg agactgtcgg
     1441 gtgagactga aaaaatggcc tccgacgcgt tgatcgcgga gataaaagca ctcagggagg
     1501 aggtggccgg gctacggcag gaaatgcagt ccatgagact gctggaacac aaaccggaga
     1561 cagcagagac cccggccgga acaccgcgcc ggtggtggcc gtggggaaaa cgctgacgcg
     1621 ctgtgcttgt ccaaccggcg acggccacgc aatgaattgc gtccccgtca ccggttatta
     1681 gagaacaata tggggattct ggcgctggcc gaccacggcc agaacccgga cttttctggt
     1741 ctggtcatca acatcataga ggacatgctg tcccatcaga ctgatccggc gaacgtcctc
     1801 gccatagcgt gagtccactt catagagtcg cgggaagtcg gccagtttgt ccgccgcagc
     1861 gagaacccgg tcggtaacgt tctgcgcatc ccacagggat gaattttccg cgatatagtc
     1921 aaacagcgtc gttacatcct cgatggctcc ctgcttccat tccacgtcat acggcatcgc
     1981 gcagttcctg ataacggcca tgcaggcgct ggcggagttg ttccgtggtc atacccggac
     2041 gcgtgtcagc acgggcggcg gtcactttgg cgcgaacctg atcttcatac tctgcctggg
     2101 ccagttcttc gttagccagt cgctgcaaca gcgcagcttt gtccgggcca ccttccggca
     2161 gtatgcgggg caggctttca gccagttccc gaagaaagtt ggcgttttcg tagtgtgtag
     2221 tgttgctcat tgcggagcct cccgggttaa tgcggcaata tgaaggaagt ttagcagggg
     2281 gcggtctgaa agaatatggt cgtgtcagtc aggctgatga agcagaaagt cggtaaaaaa
     2341 gggcttacgg catccatttt acgtcaaaac atatgccatg cagttgcgtg cataggcatg
     2401 cattaggata aaatttatcg ggcgcgtttc cggcagtttt ccgggggggt tgttgcctgt
     2461 tttatcccgt agccgccgga aacgccctga gcccgtctga gcggtgcgcg taatgacgcg
     2521 ttatggtaaa tccacttact gtctgccttc atagccatcg agataaacag ctgaaatggt
     2581 gtcagccagt agccttgatt ttttcggtgt gaggtctttg ctgcggtaat cggtacccgc
     2641 aaattgtgtt gtgccttgga gtgagtttaa aaattcctga ctgtgaaact gctgaatgcg
     2701 ttgctgcaca tcactccgtg attttccggt cgctttatcc tttgcgccca tatcccagaa
     2761 ctgccggaaa aaaggcacca tcattcgtgt tgtgtcagct ttctgggtgg aaaaattggg
     2821 atcaaaaccg tcatctgaag catatacgta atgccgggta tgctgttcca cgctgccctt
     2881 tggagcacag gcagtgagag ttgctgtcgc tgttatcatg gcgataagac acagtttgct
     2941 gccggaacgt ctcatgagcg tctccttacc tttaattctt ttttctcatt gagttatata
     3001 tccgcaggct atcttatcat cagtttctga ccgtgttcag tgaccgaaca acccgaacca
     3061 gaccccccgt ttcttttcct gtcccggagg gggctttctg gtatctcata taatgatttc
     3121 agtaaaacat actgaggccg gagaacggcg ccgtttgtgc tggtttttgt tgcatctgga
     3181 ggggattgcg catggtgtga gtggctgttt ttttgatcat tttcacaaaa acgcaaattt
     3241 acagacttga catgaaaaac aaggcagcgt aatttttata ctgtatataa aaccagtggt
     3301 tatatgtaca gtattttatt ttagtttatt gttttaaaag tcaaagagga aaagtatatg
     3361 agtggcggag acggacgagg tccgggtaat tcaggtctgg gacacaatgg tggtcaggcc
     3421 agtgggaatg tgaacggtac atcaggtaaa ggtggccctt catcaggtgg tggtacggat
     3481 ccaaacagcg ggccgggctg gggtacgacg catacaccta acggggatat tcataactat
     3541 aacccggggg agtttggtaa cggcgggagt aaacccggtg gtaatggcgg taacagcggc
     3601 aatcatagcg ggagctctgg tggcggacag tcttcggcca ccgcgatggc cttcggtctg
     3661 cctgccttgg ccactccggg ggctgaagga ctggctttat ccgtttccgg cgatgcgttg
     3721 tcggccgctg ttgctgatgt gctggctgcc ctgaaaggtc cgtttaagtt tggtctgtgg
     3781 gggattgcga tttacggagt gctgccttct gagatagcaa aagatgatcc gaaaatgatg
     3841 tcaaaaatcg tgacgtcatt gccggcagac acggtaacgg agacgccggt cagctccctg
     3901 ccgctggacc aggcgacggt cagcgtcact aaacgtgtgg cggatattgt gaaggacgag
     3961 cggcagcata ttgcggttgt caccggccgg ccaatgagtg ttcctgtggt ggatgcgaaa
     4021 ccgacaaaac gtccgggggt attcagtgtg tcgattccgg gtctcccgtc tctgcaggtg
     4081 agcgtaccta aaggtgttcc ggccgcgaaa gccccgccaa aaggcattat tgctgaaaaa
     4141 ggtgattcac gtccggctgg ttttacggcc ggtggtaact cccgtgaagc cgttattcgt
     4201 ttcccgaaag agagcggaca gaaaccggtc tatgtgtcgg tgacggatgt tcttactccg
     4261 gcacaggtaa aacagcgtct ggaggaagaa aagcgtcgcc agcaggcatg ggacgctgct
     4321 cacccggaag aggggctgaa aagagagtat gataaagcga aagctgagct ggatgctgaa
     4381 gataaaaata ttgcgacctt aaacagtcgc attgcatcga cagagaaggc gatccccggt
     4441 gcaagggctg ctgttcagga agccgataaa aaggtaaaag aggcagaggc aaataaagat
     4501 gattttgtga cttataaccc gcctcatgaa tatggctccg ggtggcagga tcaggttcgc
     4561 tatcttgata aggatattca gaatcagaat gagaaattaa aagctgccca gacatcttta
     4621 aacgaaatga atgaatcctt atccagggat aaggctgcgc tttccggggc gatggagagc
     4681 cggaaacaaa aggagaaaaa agcgaaggat gcagaaaata aattaaatga ggaaaagaaa
     4741 aaacctcgta agggaactaa agattacggc catgattatt ttcctgatcc caagactgaa
     4801 gatattaagg ggttgggaga gttgaaagag ggtaaaccta aaacccctaa acaaggtggt
     4861 ggtggtaagc gggcccgatg gtatggtgat aaaaagcgta aaatttatga atgggattcc
     4921 cagcacggtg agcttgaagg gtaccgcgcc agtgatggcg aacacctcgg ggcattcgat
     4981 ccaaaaacgg gtaagcaggt taaagggccg gatccaaaac gaaacattaa aaaatatctt
     5041 taagaggtaa atatggggct taaattacat attcattggt ttgataagaa aaccgaagag
     5101 tttaaaggtg gtgaatactc aaaagacttc ggtgatgatg gttctgtcat tgaaagtctg
     5161 gggatgcctt taaaggataa tattaataat ggttggtttg atgttgaaaa atcatgggtt
     5221 tcgatattac agccacactt taaaaatgta atcgatatta gtaaatttga ttactttgta
     5281 tcctttgttt accgggatgg taactggtaa gcaaaagtta tcaaggatga tttgaaatac
     5341 agacttccgg tgttcaggga tgaataccgg ggtttttaaa tacagggaag gtatatgaaa
     5401 aaggtaaagg ttgtttttct ttttatattg attttttcag gctttcttct ggtcgcatgt
     5461 caggcaaact atatccggga tgttcagggt ggaacggtgg caccatcgtc ctcctctgaa
     5521 ctgacgggga tcgcggttca gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     5581 tgcgcgtaat cttttgccct gtaaacgaaa aaaccacctg gggaggtggt ttgatcgaag
     5641 gttaagtcag ttggggaact gcttaaccgt ggtaactggc tttcgcagag cacagcaacc
     5701 aaatctgtcc ttccagtgta gccggacttt ggcgcacact tcaagagcaa ccgcgtgttt
     5761 agctaaacaa atcctctgcg aactcccagt taccaatggc tgctgccagt ggcgttttgc
     5821 gtgcttttcc gggttggact caagtgaaca gttaccggat aaggcgcagc agtcgggctg
     5881 aacggggggt tcttgcttac agcccagctt ggagcgaacg acctacaccg agccgagata
     5941 ccagagtgtg agctatgaga aagcgccaca cttcccgcaa gggagaaagg cggaacaggt
     6001 atccgggaaa cggcagggtt ggaacaggag agcgcaagag ggagcgattc atcggaaacg
     6061 gtgatgatct ttaagtcctg tcgggtttcg ccactcctga ctgattcatg gttgagccac
     6121 cggctcccac agatgcaccg aaaaagcgtc tgtttatgtg aactcagtca ggagggcgga
     6181 gcctatggaa aaacgccacc tgcgtggcct tgccgttttg cctcacatgt tagtcccctg
     6241 cttattcacg gaatctgtgg gtaactttgc atgtgtccgc agcgcccgcc gcagtctcac
     6301 ggccggagcg tagcgaccga gtgagcgagg aggcgcaatt tccccggcac ctacaagacg
     6361 gacaccggac gcgctgatgt ggcatattgt cccctgatta agtctttata ttccctgata
     6421 ataaattacc aggataggtg aatcatatcg gtttttcgct gaacatttta cgttaaccta
     6481 tctctgttgc ttttttctcc ttacggagaa atacaggtca tccccgcgag ggcgtggctg
     6541 acctcagggg gatatcccct gcttacgctg gctggacgcc accgacgcac ggtctctccc
     6601 cctctttctg gctccgtccg gctggacgcc ggccacgcca gaaattcacc cggcccccgt
     6661 caggctcacg cctgaccacc cacaaaaatc cacggtgctg gacgcacctg gcgaagcagc
     6721 aaagctgctc ctgattttgc gggtggtcag ccgctcgcta tggcgaactc tgacgggggc
     6781 cggaacaccg gaaaagaccg cactggggtg ccgccttttc ctctcacacg ctggggcgtg
     6841 ctcgttgttc ctccggggct gcgccttttc cgcatggggc ggaaagaggc tggcctccgt
     6901 ggtgaaagct gaacgcaatc accacggacg tgaccgccgc cgccatgcct tggggcatgt
     6961 ccgcggcttg tgcagcgggg gctgcacatc acgccggcaa accatcgtct gaaggagaga
     7021 gaacatgttt aatacggatt cgcttgcctg gaagtggcac attccggagc tggcccggca
     7081 gatttatccg tgggtcagct ggctgtacca ggagtattac ggtctggccg gggctttcct
     7141 agttatcggc ctgtttttcg gtctggctga actttgggcc ggacggatgc gttttgtggg
     7201 gctgagtcat aaggagcgac gggagtatgc cgatcgctat aagcgcgata ataaacggcc
     7261 agcgccattt tcggtgctgg cctcgctgga ttcgcccggg cggatggtca gggcacgtcg
     7321 acatgtctgg aactacgcgt actggctgac cggcgcggcc ggtctggccc ttctgggggg
     7381 gctggttgtc gccgtgttta tcgagagctg gggagaaacc gtcctgcgtc cggacctcta
     7441 cgcaaacgca gattattacc tgctgcagcc ggctaacctg ttcgggattg cctggtgcgc
     7501 gggcgggctg gctgtcggga ttgtgttggc gcggctgctg gcgttcctgc tgcttgacgg
     7561 tcattttgcg gctgctgacg aggcggtgaa cgcgaaactg aatcaggaga gccggcgcag
     7621 cagccagcgg acaggagaga tgaccgacgt gcgccacctg catttcgggg agccggtacc
     7681 ggttaatgcg ctggccgatt tttcctcgga gcaggcccgg caggagcagg cggtgtttct
     7741 gggtaaggat gagcagggca agccggtgct ggttccgcgt gatacctggc gcaagaccaa
     7801 cattcagatc ctcggcctgc ccgggagcgg caagagcgtg atggccacca acgcgctcat
     7861 tcgctgcgta cgcgatttcg gggacgcggt ggtgtacttc gaccccaaag gggacgcatg
     7921 ggcgccgcac gtcttccggg cgcactgtcc ggacttcacc ctgctggatc tgcggccggg
     7981 gaaaccggcg cagctgaatc tgttccgcga tctcgaccag tacgcgctga aaaacctgct
     8041 gattgccggg cttaacctgg gcgagaccgg cgacgtggcg gaccactacc gcatcagcga
     8101 gcagaaagcg gcgaagctga ttgcggagca gtttccgcag ggggcaaaca ttcagcaggt
     8161 gctggcggcg gcgtacgcgc tgccggaggc cctgaaaaag gacgtgaagg ggttgatcac
     8221 taggctggaa aacgtcgctg acctgagcgt cctgcagacg gacagtggca tcgatgtggc
     8281 ggggattatc aacggcggcg gctgcctgta cgttatcggc tcgatggacg acgaggcggt
     8341 gatccgtgtg caaaaaatgc tgtttgcccg ctgcgcgcaa atcatcattg cgcgggacga
     8401 gttccgggag tggccacacg tcagcgtgat gctcgatgaa attaaatatc tgctgtcgaa
     8461 gtatgtactg aacgcactgg ggacgctgcg cagccgggac tgcaatctgt tgctcgcgca
     8521 ccagtcgctg ggcgatttcg ggcagtgcgg gcaggatctg cccgctgaat ttgtgaaaac
     8581 cacggtactg gataacacgc cgatccgctg gttttaccgg gcggccagcc tggagtccgc
     8641 gcagtgggcg gcggggcaga ccggggaaat cagggtggac gtggagcgcc gccgtgccag
     8701 ccgggaggcg gggaacgtgg agcatatcag cggggacagc ttcatccaga aggaggcgcg
     8761 gccgctgttt gacgtgaaca ccctgcaaca cctgccggac gggttcgcgg tcatgaccgg
     8821 gctgggtgtg gcccggatgg cgttcagcag tccgctgcgg gttgagcgcc gggaaatccc
     8881 gttgcagcgt ttcccggtgc tggcgaaaac cgatcccctg gcggagttcc ggcccccggc
     8941 cgacggcggc gggccgaaag cggatgatga ttttgcgggg atttactgat ggcgctggag
     9001 ctgtacaacg tcagccacgc gaaacgacag gcgcggaacg cggagaaaac ccggctgacc
     9061 ctgcgctggt tgcgggaaga gctgtgttcc acggcggagc tggttgcgcg gcggctgggg
     9121 attgcggcgg tgcagccggt ctaccgcttc ctcgacagcc tggtggcgaa agggctgctg
     9181 gtccggacca aatatccggt ggacggccgt caggtcagcg tgtgggggct gacgccgcat
     9241 ggcgtggcgt tctcgttcga cgaggacgaa ccgctggcgg atgttattcc gttt
//
