LOCUS       NZ_CP033779             2995 bp    DNA     linear   CON 08-JAN-2024
DEFINITION  Klebsiella pneumoniae strain FDAARGOS_531 plasmid unnamed5.
ACCESSION   NZ_CP033779
VERSION     NZ_CP033779.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN10163226
            Assembly: GCF_003812105.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 2995)
  AUTHORS   Campos,J., Goldberg,B., Tallon,L., Sadzewicz,L., Zhao,X.,
            Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S., Geyer,C.,
            Nandy,P., Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2995)
  AUTHORS   Campos,J., Goldberg,B., Tallon,L., Sadzewicz,L., Zhao,X.,
            Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S., Geyer,C.,
            Nandy,P., Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2018) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, Silver
            Spring, MD 20993-0002, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SMRT v. 2.3.0, HGAP v. 3.0, SPAdes v. 3.6.0
            Genome Coverage                   :: 620.460689310345x
            Sequencing Technology             :: PacBio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/08/2024 04:11:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,634
            CDSs (total)                      :: 5,510
            Genes (coding)                    :: 5,349
            CDSs (with protein)               :: 5,349
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 161
            CDSs (without protein)            :: 161
            Pseudo Genes (ambiguous residues) :: 0 of 161
            Pseudo Genes (frameshifted)       :: 91 of 161
            Pseudo Genes (incomplete)         :: 81 of 161
            Pseudo Genes (internal stop)      :: 21 of 161
            Pseudo Genes (multiple problems)  :: 29 of 161
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP033779.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2995
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_531"
                     /isolation_source="Bronchoalveolar lavage from right lung"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_531"
                     /db_xref="taxon:573"
                     /plasmid="unnamed5"
                     /collection_date="2016-04-07"
                     /collected_by="Children's National Hospital"
     gene            complement(394..657)
                     /locus_tag="EGX99_RS28955"
                     /old_locus_tag="EGX99_28895"
     CDS             complement(394..657)
                     /locus_tag="EGX99_RS28955"
                     /old_locus_tag="EGX99_28895"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_023237785.1"
                     /translation="MISKVLWADKALSDRETIFESNDKVSGLSYAKSEDAKIKRAVSTI
                     KRNNKVGRNDLDERGYVYVLPKRYKILYRLTEEHIFIERVFY"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(669..1064)
                     /locus_tag="EGX99_RS28960"
                     /old_locus_tag="EGX99_28900"
     CDS             complement(669..1064)
                     /locus_tag="EGX99_RS28960"
                     /old_locus_tag="EGX99_28900"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032435811.1"
                     /translation="MAFVSQVDKSRAASSRIGDSSIVVTQSYKASTKAQELSIRLTSLV
                     LSKGGMKIGDYVDVLYDKDSDRWMLRKMDGGLKITGKDDAPTGLIRYTLKDTHARFTED
                     KEHLPIRKSSIEDSLEIEDGQIVFKLD"
                     /name="hypothetical protein"
     gene            1299..1574
                     /locus_tag="EGX99_RS28965"
                     /old_locus_tag="EGX99_28905"
     CDS             1299..1574
                     /locus_tag="EGX99_RS28965"
                     /old_locus_tag="EGX99_28905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004099076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="WP_224359394.1"
                     /translation="MQFRIESETKKMAKQALEKKGISLSDALRAFLDKLAATEKVMTKE
                     ETWLKEQIEETFSRVDKGEIRYYSEDEADERMNSFISKIEHQHETA"
                     /name="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
     gene            complement(2378..2527)
                     /locus_tag="EGX99_RS29580"
     CDS             complement(2378..2527)
                     /locus_tag="EGX99_RS29580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023237787.1"
                     /translation="MPSEHDEQRDVADTQLPGEYDLFDLMAKVTEENLHAPVDSGPPVG
                     REAL"
                     /name="hypothetical protein"
     gene            2658..>2995
                     /locus_tag="EGX99_RS29615"
     CDS             2658..>2995
                     /locus_tag="EGX99_RS29615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_123806936.1"
                     /translation="MWQEKNAAAERKQNMRYRRYSASSLTDSLRSVVRLRRAVSAYRRG
                     GDFLEDAKKKLNREAIRPRRSRFSIGSAPLTSITESDTQISGGETRQDYKDTRRFPLMA
                     PSCALLFLP"
                     /name="hypothetical protein"
     Typing          complement(2562..2734)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="87.931"
                     /coverage="100.0"
                     /evalue="9.61e-51"
ORIGIN
        1 cacgcctgac actcagttcc gggtaggcag ttcgctccaa gctggactgt atgcacgaac
       61 cccccgttca gtccgacccc cacgacaaac aagcaactcg actcttgaat ccaatcttgt
      121 ttgactcaaa ttatgacaat ggtggaagcc tgtaataact gatttgcgga gcgtagaaag
      181 aaggtaagca aggatatctt gaagtgagca gtgaactacg gctacactga aagaacagtt
      241 ttggtgactg cgctccgcga aagccagtta ccacaagtcc aatgagttgg tagctcaaag
      301 gactacgaag aaaccaccgt tggtagcggt ggttttcttc atcccaagat taatagctaa
      361 agagacgcgt agtcaagaaa ggtcgccaaa ccatcagtag aaaacgcgct caataaaaat
      421 atgctcctca gtcagccgat acaaaatctt gtatctttta ggtagaacat agacataacc
      481 tcgctcatcc aggtcatttc tgcctacttt gttgtttcgt tttatcgtag aaacagcacg
      541 tttaattttt gcatcttctg attttgcata cgacaaacca gaaaccttat cgttagactc
      601 aaagatagtc tctcgatcag ataatgcctt atctgcccat agaaccttag agatcatcaa
      661 acctcccgct agtcaagctt aaatacaatc tgcccatcct caatctccag tgaatcctca
      721 atagaggatt tacgaatagg gaggtgctct ttatcttcgg taaatctcgc atgtgtatcc
      781 ttcagggtat atctaatcaa ccccgtaggg gcatcatcct taccagtaat ttttaaacct
      841 ccatccatct tccttaacat ccagcgatca ctatccttgt cataaagaac atcaacgtaa
      901 tcaccaattt tcatacctcc cttagataac actaacgatg ttaatctaat ggatagctcc
      961 tgagccttag ttgaagcctt gtatgattga gtgaccacta tagacgaatc accaatcctg
     1021 ctcgaagcag ccctagactt atcaacctga ctaacaaatg ccataaaacc tcctgatagt
     1081 ttattttcat gttcataata cgatcgacaa aaaatcttag caacacagat ctatgttttt
     1141 agctgatctt tatcctctct cagaatccga agaaaaaaag ttactttttc taatcataaa
     1201 tctaagaaag gaggcattga ctgtagggat cccagcgtct attgtgagca caaacgtact
     1261 cacaatagac gctgggatct atatctatgg aaacaagggt gcaattcaga attgaatctg
     1321 aaacaaagaa aatggcaaag caagcacttg aaaaaaaagg catttcactt agcgatgcgt
     1381 taagagcgtt tctcgataag ttagcagcaa cggagaaggt tatgacgaaa gaagagacat
     1441 ggctgaaaga gcagatagaa gagacattct caagagtaga caaaggagag atccgctatt
     1501 actcagaaga tgaagctgac gaacgcatga actccttcat ctctaaaatc gaacatcaac
     1561 acgaaaccgc ataaaaaaag ccgccttatg gcggcttttt ttatgccctt atttaccata
     1621 acaccctaat tgtacgcacc gccaaaacgg cctcagggcg tttctgacag gaaacaggta
     1681 aaacagacaa caaactatca gaaaatcagc caaaaacacg ttagttctgt tttcacgtca
     1741 tgcatgagta tgcgcgcaag cgcatagcgc ggaaattcac gccattcagc cggggtaagt
     1801 cctgtttccg atgttcttct tacccgaaaa cggtgaacgg ggacgcaatt tattgcgtag
     1861 ccatgaggca ttttgccggc acagccggaa aaaccatttt cagcacgtgc aggaagaggg
     1921 ggggcagatg tacgaatagt tcagaggtca gatctgacgt aagaatgatg taaaagtgat
     1981 gtaattctgt agttgatttg tagtttatct gtagttagcc ctctgaaatg catgattaaa
     2041 catcagcttt tgtagtcttt ttgtagttta cctgtagtca ggaaaactac tccgttcatg
     2101 ctcgcatgaa cggagtagtg aaggacgctt ttttgtcata tcagcctctt aacctgcgca
     2161 gatggcccag tcaaaatgat gattttttaa cccttaaatg gtcatacacc taacgcagcc
     2221 gcgattttca tcgcaactgc atgaattaca taaaaaaaat gagatactcc atttgtcacg
     2281 gtaatacgca aggtattaca atcaactgta aatacatgaa aataaagaaa taagatgtaa
     2341 taccaccgta ttacacgtac gctgcccagc gaaaaagcta taacgcttcc cggccgacag
     2401 gtggcccgga atccaccggg gcgtgcaggt tctcttcagt aaccttcgcc attaaatcaa
     2461 aaaggtcata ctcgccggga agctgcgtat cagcaacatc ccgttgttca tcatgttcag
     2521 atggcatggg gtgatatcca gtagctgaga ggttgttagc gggttccggg cgcagcgccg
     2581 gaccagtcac gtagcgctag cggagtgtat actggcttag tatgttggca tgagtgcgat
     2641 aatcaggaaa gcgccttatg tggcaggaga aaaatgctgc agcagagcgt aagcagaata
     2701 tgcgatacag aagatattcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     2761 tgcggcgagc ggtatcggct tacagacggg gcggagattt cctggaagat gccaagaaga
     2821 aacttaacag ggaagcgata aggccacggc ggagccgttt ttccataggc tccgcccccc
     2881 tgacaagcat cacagaatct gacactcaaa tcagtggtgg cgaaacccga caggactata
     2941 aagataccag gcgtttcccc ctgatggctc cctcgtgcgc tctcctgttc ctgcc
//
