LOCUS       NZ_CP033733             4439 bp    DNA     circular CON 29-MAR-2024
DEFINITION  Staphylococcus hominis strain FDAARGOS_575 plasmid unnamed2,
            complete sequence.
ACCESSION   NZ_CP033733
VERSION     NZ_CP033733.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN10163251
            Assembly: GCF_003812505.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus hominis
  ORGANISM  Staphylococcus hominis
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4439)
  AUTHORS   Hart,M., Basco,M., Tallon,L.J., Sadzewicz,L., Zhao,X.,
            Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Agnihothram,S., Nandy,P., Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4439)
  AUTHORS   Hart,M., Basco,M., Tallon,L.J., Sadzewicz,L., Zhao,X.,
            Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Agnihothram,S., Nandy,P., Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2018) Center for Devices and Radiological Health,
            US Food and Drug Administration, 10903 New Hampshire Avenue, Silver
            Spring, MD 20993-0002, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 2.3.0, HGAP v. 3.0
            Genome Coverage                   :: 2300.03357478261x
            Sequencing Technology             :: PacBio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_003812505.1-RS_2024_03_28
            Annotation Date                   :: 03/28/2024 04:24:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,262
            CDSs (total)                      :: 2,177
            Genes (coding)                    :: 2,142
            CDSs (with protein)               :: 2,142
            Genes (RNA)                       :: 85
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 62
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 12 of 35
            Pseudo Genes (incomplete)         :: 24 of 35
            Pseudo Genes (internal stop)      :: 13 of 35
            Pseudo Genes (multiple problems)  :: 11 of 35
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP033733.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4439
                     /organism="Staphylococcus hominis"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_575"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_575"
                     /db_xref="taxon:1290"
                     /plasmid="unnamed2"
                     /collected_by="FDA-NCTR"
                     /note="Clinical Isolate"
     gene            10..1389
                     /gene="tet(K)"
                     /locus_tag="EGX58_RS11210"
                     /old_locus_tag="EGX58_11220"
                     /db_xref="GeneID:39460882"
     ARG             11..1386
                     /name="tet(K)"
                     /gene="tet(K)"
                     /gene_name="tetracycline efflux MFS transporter Tet(K)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            1575..2816
                     /gene="mobV"
                     /locus_tag="EGX58_RS11215"
                     /old_locus_tag="EGX58_11225"
                     /db_xref="GeneID:39460881"
     CDS             1575..2816
                     /gene="mobV"
                     /locus_tag="EGX58_RS11215"
                     /old_locus_tag="EGX58_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010889940.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_000122374.1"
                     /db_xref="GeneID:39460881"
                     /translation="MSYSIVRVSKVKSGTNTTGIQKHVQRENNNYENEDIDHSKTYLNY
                     DLVNANKQNFNNLIDEKIEQNYTGKRKIRTDAIKHIDGLITSDNDFFDNQTPEDTKQFF
                     EYAKEFLEQEYGKDNLLYATVHMDEKTPHMHYGVVPITDDGRLSAKEVVGNKKALTAFQ
                     DRFNEHVKQRGYDLERGQSRQVTNAKHEQISQYKQKTEYHKQEYERESQKTDHIKQKND
                     KLMQEYQKSLNTLKKPINVPYEQETEKVGGLFSKEIQETGNVVISQKDFNEFQKQIKAA
                     QDISEDYEYIKSGRALDDKDKEIREKDDLLNKAVERIENADDNFNQLYENAKPLKENIE
                     IALKLLKILLKELERVLGRNTFAERVNKLTEDEPKLNGLAGNLDKKMNPELYSEQEQQQ
                     EQQKNQKRDRGMHL"
                     /name="mobV"
     gene            3343..4287
                     /locus_tag="EGX58_RS11220"
                     /old_locus_tag="EGX58_11230"
                     /db_xref="GeneID:39460880"
     CDS             3343..4287
                     /locus_tag="EGX58_RS11220"
                     /old_locus_tag="EGX58_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277654.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_000277654.1"
                     /db_xref="GeneID:39460880"
                     /translation="MYKNNHANHSNHLENHDLDNFSKTGYSNSRLDAHTVCISDPKLSF
                     DAMTIVGNLNRDNAQALSKFMSVEPQIRLWDILQTKFKAKALQEKVYIEYDKVKADSWD
                     RRNMRIEFNPNKLTRDEMIWLKQNIISYMEDDGFTRLDLAFDFEDDLSDYYAMSDKAVK
                     KTIFYGRNGKPETKYFGVRDSNRFIRIYNKKQERKDNADAEVMSEHLWRVEIELKRDMV
                     DYWNDCFSDLHILQPDWKTIQRTADRAIVFMLLSDEEEWGKLHRNSRTKYKNLIKEISP
                     VDLTDLMKSTLKANEKQLQKQIDFWQHEFKFWK"
                     /name="replication initiation factor domain-containing
                     protein"
     Typing          3344..4287
                     /name="rep_cluster_1118"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          1576..2813
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gaggtaaaat tgtttagttt atataaaaaa tttaaaggtt tgttttatag cgttttattt
       61 tggctttgta ttctttcatt ttttagtgta ttaaatgaaa tggttttaaa tgtttcttta
      121 cctgatattg caaatcattt taatactact cctggaatta caaactgggt aaacactgca
      181 tatatgttaa ctttttcgat aggaacagca gtatatggaa aattatctga ttatataaat
      241 ataaaaaaat tgttaattat tggtattagt ttgagctgtc ttggttcatt gattgctttt
      301 attggtcaca atcacttttt tattttgatt tttggtaggt tagtacaagg agtaggatct
      361 gctgcattcc cttcactgat tatggtggtt gtagctagaa atattacaag aaaaaaacaa
      421 ggcaaagcct ttggttttat aggatcaatt gtagctttag gtgaagggtt aggtccttca
      481 atagggggaa taatagcaca ttatattcat tggtcttacc tacttatact tcctatgatt
      541 acaatagtaa ctataccttt tcttattaaa gtaatggtac ctggtaaatc aacaaaaaat
      601 acattagata tcgtaggtat tgttttaatg tctataagta ttatatgttt tatgttattt
      661 acgacaaatt ataattggac ttttttaata ctcttcacaa tcttttttgt gatttttatt
      721 aaacatattt caagagtttc taaccctttt attaatccta aactagggaa aaacattccg
      781 tttatgcttg gtttgttttc tggtgggcta atattttcta tagtagctgg ttttatatca
      841 atggtgcctt atatgatgaa aactatttat catgtaaatg tagcgacaat aggtaatagt
      901 gttatttttc ctggaaccat gagtgttatt gtttttggtt attttggtgg ttttttagtg
      961 gatagaaaag gatcattatt tgtttttatt ttaggatcat tgtctatctc tataagtttt
     1021 ttaactattg cattttttgt tgagtttagt atgtggttga ctacttttat gtttatattt
     1081 gttatgggcg gattatcttt tactaaaaca gttatatcaa aaatagtatc aagtagtctt
     1141 tctgaagaag aagttgcttc tggaatgagt ttgctaaatt tcacaagttt tttatcagag
     1201 ggaacaggta tagcaattgt aggaggttta ttgtcactac aattgattaa tcgtaaacta
     1261 gttctggaat ttataaatta ttcttctgga gtgtatagta atattcttgt agccatggct
     1321 atccttatta ttttatgttg tcttttgacg attattgtat ttaaacgttc tgaaaagcag
     1381 tttgaatagt tatattatat tttggtttag aactatgagt ggctagcatt ttgccactca
     1441 ttttttgcgt tagcaaaaac aggtttaagc ctcgcagagc acacgtatta acgacttatt
     1501 aaaaataagt ctagtgtgtt agacttaaac tattaaatac acatgaaacc tttgtgctta
     1561 ggagtgattt ttatatgtct tattccattg ttagagtttc aaaagttaaa tctggaacaa
     1621 atacaacggg catacaaaaa catgttcaaa gagaaaataa taattatgaa aatgaagata
     1681 tagaccatag taaaacttac ttaaattatg atttggtaaa tgctaataaa cagaatttta
     1741 ataacttgat tgatgaaaaa atcgaacaga attatacagg caaaagaaaa attagaacag
     1801 acgcgattaa acacattgat ggtttaatta catcagacaa tgatttcttt gataatcaaa
     1861 cgccagaaga tacaaagcag ttttttgaat atgctaaaga gtttttagaa caagaatacg
     1921 gtaaagataa tttattatat gcaacagttc acatggacga aaaaacacca catatgcatt
     1981 atggcgttgt tccaataact gatgatggtc gtttaagtgc taaagaagtt gtaggtaata
     2041 aaaaagcttt aacagcgttt caagatagat ttaatgagca tgttaaacaa cgaggatatg
     2101 atttagaacg tgggcaatca agacaagtaa caaatgctaa acatgagcaa ataagtcagt
     2161 ataaacaaaa aacagaatat cataagcaag aatatgaacg tgagagccaa aaaacagacc
     2221 atataaagca aaagaacgat aaattaatgc aagagtacca aaaatcgtta aatacgctta
     2281 aaaagcctat aaatgttccg tatgagcaag aaactgaaaa agtaggtggt ttatttagca
     2341 aagaaataca agaaactgga aatgttgtaa taagccaaaa agatttcaat gaatttcaga
     2401 aacagataaa agctgctcaa gatatttcgg aagattacga gtatataaag tctggtagag
     2461 ccttagatga taaagataag gaaatacgag agaaagatga tttattaaat aaagcagttg
     2521 agcgtattga aaacgcagac gataatttta accaacttta cgaaaatgca aagccactta
     2581 aagagaatat agaaatagcg ttaaagcttt taaaaatctt actaaaagag ttagaacgag
     2641 ttttaggaag aaataccttt gcggaaagag ttaataagtt aacagaagat gaaccaaaac
     2701 taaatggttt agcaggaaac ttagataaaa aaatgaatcc agaattatat tcagaacagg
     2761 aacagcaaca agaacaacaa aagaatcaaa aacgagatag aggtatgcac ttatagaaca
     2821 tgcatttatg ccgagaaaac ttattggttg gaatgggcta tgtgttagct aacttgttag
     2881 cgagttggtt ggacttgaat tgggattaat cccaagaaag taccaactca acaacacata
     2941 aagccctgta ggttccgacc aataaggaaa ttggaataaa gcaataaaag gagttgaaga
     3001 aatgaaattc agagaagcct ttgagaattt tataacaagt aagtatgtac ttggtgtttt
     3061 agtagtttta actgtttacc agataataca aatgcttaaa taaaaaaaga cttgatctga
     3121 ttagaccaag tcttttgata gtgttatatt aataacaaaa taaaaaggag tcgctcacgc
     3181 cctgaccaaa gtttgtgaac gacatcattc aaagaaaaaa acactgagtt gtttttataa
     3241 tcttgtatat ttagatatta aacgatattt aaatatacat caagatatat atttgggtga
     3301 gcgattcctt aaacgaaatt gagattaagg agtcgatttt ttatgtataa aaacaatcat
     3361 gcaaatcatt caaatcattt ggaaaatcac gatttagaca atttttctaa aaccggctac
     3421 tctaatagcc ggttggacgc acatactgtg tgcatatctg atccaaaatt aagttttgat
     3481 gcaatgacga tcgttggaaa tctcaaccga gacaacgctc aagccctttc taaatttatg
     3541 agtgtagagc cccaaataag actttgggat attcttcaaa caaagtttaa agctaaagca
     3601 cttcaagaaa aagtttatat tgaatatgac aaagtgaaag cagatagttg ggatagacgt
     3661 aatatgcgta ttgaatttaa tccaaacaaa cttacacgag atgaaatgat ttggttaaaa
     3721 caaaatataa taagctacat ggaagatgac ggttttacaa gattagattt agcctttgat
     3781 tttgaagatg atttgagtga ctactatgca atgtctgata aagcagttaa gaaaactatt
     3841 ttttatggtc gtaatggtaa gccagaaaca aaatattttg gcgtgagaga tagtaataga
     3901 tttattagaa tttataataa aaagcaagaa cgtaaagata atgcagatgc tgaagttatg
     3961 tctgaacatt tatggcgtgt agaaatcgaa cttaaaagag atatggtgga ttactggaat
     4021 gattgcttta gtgatttaca tatcttgcaa ccagattgga aaactatcca acgcactgcg
     4081 gatagagcaa tagtttttat gttattgagt gatgaagaag aatggggaaa gcttcacaga
     4141 aattctagaa caaaatataa gaatttgata aaagaaattt cgccagtcga tttaacggac
     4201 ttaatgaaat cgactttaaa agcgaacgaa aaacaattgc aaaaacaaat cgatttttgg
     4261 caacatgaat ttaaattttg gaaatagtgt acatattaat attactgaac aaaaatgata
     4321 tatttaaact attctaattt aggaggattt ttttatgaag tgtctattta aaaatttggg
     4381 gaatttatat gaggtgaaag aataatttac ccctataaac tttagtcacc tcaagtaaa
//
