LOCUS       NZ_CP033403            10060 bp    DNA     circular CON 26-NOV-2023
DEFINITION  Klebsiella pneumoniae strain WCHKP115069 plasmid p2_115069, complete
            sequence.
ACCESSION   NZ_CP033403
VERSION     NZ_CP033403.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN09688181
            Assembly: GCF_003711065.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10060)
  AUTHORS   Hu,Y., Feng,Y. and Zong,Z.
  TITLE     The complete genome of Klebsiella pneumoniae strain WCHKP115069
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10060)
  AUTHORS   Hu,Y., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-OCT-2018) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/26/2023 02:16:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,806
            CDSs (total)                      :: 5,686
            Genes (coding)                    :: 5,535
            CDSs (with protein)               :: 5,535
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 56 of 151
            Pseudo Genes (incomplete)         :: 99 of 151
            Pseudo Genes (internal stop)      :: 18 of 151
            Pseudo Genes (multiple problems)  :: 22 of 151
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP033403.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10060
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="WCHKP115069"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p2_115069"
                     /geo_loc_name="China: Chengdu, Sichuan"
                     /collection_date="2018"
     gene            join(9716..10060,1..1341)
                     /locus_tag="DVJ73_RS01295"
                     /old_locus_tag="DVJ73_01295"
     CDS             join(9716..10060,1..1341)
                     /locus_tag="DVJ73_RS01295"
                     /old_locus_tag="DVJ73_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            1351..1608
                     /locus_tag="DVJ73_RS01300"
                     /old_locus_tag="DVJ73_01300"
     CDS             1351..1608
                     /locus_tag="DVJ73_RS01300"
                     /old_locus_tag="DVJ73_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            1693..1842
                     /locus_tag="DVJ73_RS01305"
                     /old_locus_tag="DVJ73_01305"
     CDS             1693..1842
                     /locus_tag="DVJ73_RS01305"
                     /old_locus_tag="DVJ73_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            3326..5290
                     /locus_tag="DVJ73_RS01315"
                     /old_locus_tag="DVJ73_01315"
     CDS             3326..5290
                     /locus_tag="DVJ73_RS01315"
                     /old_locus_tag="DVJ73_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            5290..6021
                     /gene="mobC"
                     /locus_tag="DVJ73_RS01320"
                     /old_locus_tag="DVJ73_01320"
     CDS             5290..6021
                     /gene="mobC"
                     /locus_tag="DVJ73_RS01320"
                     /old_locus_tag="DVJ73_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            6028..6558
                     /locus_tag="DVJ73_RS01325"
                     /old_locus_tag="DVJ73_01325"
     CDS             6028..6558
                     /locus_tag="DVJ73_RS01325"
                     /old_locus_tag="DVJ73_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(6585..6764)
                     /locus_tag="DVJ73_RS01330"
                     /old_locus_tag="DVJ73_01330"
     CDS             complement(6585..6764)
                     /locus_tag="DVJ73_RS01330"
                     /old_locus_tag="DVJ73_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(6790..7218)
                     /locus_tag="DVJ73_RS01335"
                     /old_locus_tag="DVJ73_01335"
     CDS             complement(6790..7218)
                     /locus_tag="DVJ73_RS01335"
                     /old_locus_tag="DVJ73_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(7382..7696)
                     /locus_tag="DVJ73_RS01340"
                     /old_locus_tag="DVJ73_01340"
     CDS             complement(7382..7696)
                     /locus_tag="DVJ73_RS01340"
                     /old_locus_tag="DVJ73_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(7684..7962)
                     /locus_tag="DVJ73_RS01345"
                     /old_locus_tag="DVJ73_01345"
     CDS             complement(7684..7962)
                     /locus_tag="DVJ73_RS01345"
                     /old_locus_tag="DVJ73_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(8137..8502)
                     /locus_tag="DVJ73_RS31365"
                     /old_locus_tag="DVJ73_01350"
     CDS             complement(8137..8502)
                     /locus_tag="DVJ73_RS31365"
                     /old_locus_tag="DVJ73_01350"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            complement(8499..8870)
                     /gene="tolA"
                     /locus_tag="DVJ73_RS01355"
                     /old_locus_tag="DVJ73_01355"
     CDS             complement(8499..8870)
                     /gene="tolA"
                     /locus_tag="DVJ73_RS01355"
                     /old_locus_tag="DVJ73_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(9144..9389)
                     /locus_tag="DVJ73_RS01360"
                     /old_locus_tag="DVJ73_01360"
     CDS             complement(9144..9389)
                     /locus_tag="DVJ73_RS01360"
                     /old_locus_tag="DVJ73_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     BGC             1..6341
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(1946..2524)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          5291..6018
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 gtttccggcg atgcgttgtc ggccgccgtt gctgatgtgc tggctgccct gaaggggccg
       61 tttaagtttg gtctgtgggg gattgcgatc tacggtgtgc tgccttctga gattgcaaaa
      121 gatgatccga aaatgatgtc aaaaattatg acgtcattac cggccgatgc ggtgacggag
      181 actccggcaa gtactttacc actggaccag gcgacggttc gtgtcagaca acgggttgtg
      241 gatgtggtga aggatgagcg gcagcatatt gcggttgtcg caggtcggcc aatgagtgtc
      301 cctgtggtgg atgcgaaacc gacaaaacgt ccgggggtat tcagtgtgtc gattccgggt
      361 ctcccgtctc tgcaggtgag cgtaccgaaa ggtgttccga cagcgaaagc cccgccaaaa
      421 ggcattgttg ctgaaaaagg tgattcacgt ccggctggtt ttacagccgg tggtaactcc
      481 cgtgaggccg ttattcgttt cccgaaagag accggacaga agccggttta tgtgtcggtg
      541 acagatgttc ttaccccggc acaggtaaaa cagcgtcagg aggaagaaaa gcgtcgccag
      601 caggcatggg acgccgctca tccggaagag gggctgaaaa gagactatga taaagcgaaa
      661 gctgagctgg atgccgaaga taaaaatatt gcgaccttaa acagccgcat tgcatcgaca
      721 gagaaggcgc tccccggtgc aagggctgct gttcaggaag ccgataaaaa ggtaaaagag
      781 gcagaggcaa ataaagatga ttttgtgact tataacccgc ctcatgaata tggctccggg
      841 tggcaggatc aggttcgcta tcttgataag gatattcaga atcagaatga gaaattaaaa
      901 gcggctcagg catctttaaa cgcaatgaat gaatccttat ccagagataa ggctgcactt
      961 accggggcga tggagagccg gaaacaaaag gagaaaaaag cgaaggatgc agaaaataag
     1021 ttaaatgagg aaaagaacaa gcctcgcaag gggactaaag actacggcca tgattatcat
     1081 ccagtaccta aaacagaaga cataaaggga cttggagagt taaagcgagg tgatcctaaa
     1141 acacctaaac aaggtggtgg tggtaagcgg gcccgatggt atggcgataa aaaacgtaaa
     1201 atttatgaat gggattccca gcacggtgag cttgaagggt atcgtgccag tgatggcgaa
     1261 cacctaggcg catttgatcc caaaacgggt aagcaggtta aagggccaga tccaaaacgg
     1321 aacattaaaa aatatctttg agaggtaaat atggggctta aattacgttt gacatggttt
     1381 gataagaaaa ctgaagagtt taaaggggaa gagtattcta aagactttgg tgacgatggt
     1441 tctgtcattg aaagcttggg aatgccttta aaggataata ttaataatgg tgagtttgat
     1501 ttgaagaaag aatgggttcc tttactacag ccatttttta aaaatcaaat aaaacctgat
     1561 gtggataatt gttatatagc ttttgattat cgggacggta actggtaaca aaaaattatc
     1621 aaggatgatt tgaaatactg acttccggtg ttcagggatg aatgccggag tctttaaata
     1681 cagggaaagt atatgaaaaa ggtaaagact atttttcttt ttatattgat tgttgctggc
     1741 tttcttctgg ccgcatgtca ggcaaattat atccgggatg ttcagggcgg aacggtggca
     1801 ccatcgtcct cctctgaact gacggggatc gcggttcagt agaaaagatc aaaggatctt
     1861 cttgagatcc tttttttctg cgcgtaatct tttgccctgt aaacgaaaaa accacctggg
     1921 gaggtggttt gatcgaaggt taagtcagtt ggggaactgc ttaaccgtgg taactggctt
     1981 tcgcagagca cagcaaccaa atctgtcctt ccagtgtagc cggactttgg cgcacacttc
     2041 aagagcaacc gcgtgtttag ctaaacaaat cctctgcgaa ctcccagtta ccaatggctg
     2101 ctgccagtgg cgttttgcgt gctttcccgg gttggactca agtgaacagt taccggataa
     2161 ggcgcagcag tcgggctgaa cggggagttc ttgcttacag cccagcttgg agcgaacgac
     2221 ctacaccgag ccgagatacc agtgtgtgag ctatgagaaa gcgccacact tcccgcaagg
     2281 gagaacggcg gacaggtatc cggtaaacgg cagggtcgga acaggagagc gcaagaggga
     2341 gcgacctacc ggaaacggtg gggatcttta agtcctgtcg ggtttcgccc gtactgtcag
     2401 attcatggtt gagcctcacg gctcccacag atgcaccgaa aaagcgtctg tttatgtgaa
     2461 ctctggcagg agggcggagc ctatggaaaa acgccaccgg cgcggccctg ccgttttgcc
     2521 tcacatgtta gtcccctgct tatccacgga atctgtgggt aactttgtat gtgtccgcag
     2581 cgcccgccgc agtctcacgc ccggagcgta gcgaccgagt gagcgaggag gcgcaatttc
     2641 cccggcacct gcaagacggt caccggacgc gctgatgtgg gatattgtcc cctgattaag
     2701 tctttctatt ccctgacgat caattatcag gataggtgaa ccatgtcggt ttttcgctga
     2761 acattttacg ttaacctatc tctgttgctt ttttctcctt gcggagaaat gcaagtcatc
     2821 cccgctgggg cgtggctgac ttcaggggaa aagaccttgc ttacgctggc tggacgccac
     2881 cgacgcacgg tcttttcccc tctttctggc tctgtccggc tggacgccgg ccacgccaga
     2941 aattcacccg gcccccgtca ggctcacgcc tgaccaccca caaaaatcca cggtgctgga
     3001 cgcacctgac gaagcagcaa agctgctcct gattttgcgg gtggtcagcc gctcgctatg
     3061 gcgaactctg acgggggccg gaacaccgga aaagaccgca ctggggtgcc gccttttcct
     3121 ctcacacgct ggggcgtgct cgttgttcct ccggggctgc gccttttccg catggggcgg
     3181 aaagaggctg gcctccgcgg tgaaagctga acgcaatcac cacggacgtg accgccgccg
     3241 ccatgccttg gggcatgtcc gcggcttgtg cagcgggggc tgcgcatcac gccggtaaac
     3301 ccgtcgtctg aaggagagag attgcatgtt taatacggat tcgcttgcct ggcagtggca
     3361 tatcccggag ctggcccggc agatttaccc gtgggtcagc tggctgtacc gggagtatta
     3421 cagtctggcc ggtttttttc tggttatcgg cctgtttttc ggtctggctg aactctgggc
     3481 cggccgaatg cgttttgtgg ggctgagcca taaggcgcgg cgggagtatg ccgatcgcta
     3541 taagcgcgat aataaccgga ctgtgccatt ttcggtgctg gcctcgctgg attcacccgg
     3601 gcggatggtc agggcacggc gacatgtctg gaactacgcg tactggctga ccggcgctgc
     3661 cggtctggcc cttctggggg ggctggttgt cgccgtgttt atcgagagct ggggagaaac
     3721 cgtcctgcgc ccggacctct atgcgaacgc agattattac ctgctgcagc cggctaacct
     3781 gttcgggata gcctggtgtg ccggagggct tgtcgccggc gtccttctgg cgcggctgct
     3841 ggcgttcctg ctgcttgatg gtcattttgc ggccgctgac gaggcggtga acgcgaaact
     3901 gaatcaggag agccggcgca gcagccagcg gaccggagaa atgacagacg tccgccacct
     3961 gcatttcggg gagccggtac cggttaacgc gctggccgat ttttccacgg agcaggcccg
     4021 gaaacagcag gcggtgtttc tgggtaagga tgagcagggc cagccggtgc tggtccctcg
     4081 tgatacctgg cgcaagacca atattcagat cctcggcctg cccgggagcg gcaagagcgc
     4141 gatggccacc aacgcgctca ttcgctgcgt acgcgatttc ggggacgctg tggtgtactt
     4201 cgaccccaaa ggggacgcgt gggcgccgca cgtcttccgg gcgcactgcc cggacttcac
     4261 cctgctggat ctgcggccgg ggaaaccggc gcagctgaat ctgttccgcg atctcgacca
     4321 gtacgccctg aagaatctgc tggttgccgg gtttaacctg agtgagaccg gcgacgtggc
     4381 ggaccactac cgcatcagcg agcagaaagc ggcgaagctg attgcggagc agttcccgca
     4441 gggggcaaac attcagcagg tgctggcggc ggcgtacgcg ttgccggagg ccctgaaaaa
     4501 ggacgtgaag gggctgatca ccaagctgga gaacgtggcc gacctgagcg tcctgcagac
     4561 ggacagcggt atcgatgtgg cggggataat caacggcggc ggctgcctgt acgtcatcgg
     4621 ctcgatggac gatgaggcgg tgatccgggt gcagaagatg ctgtttgccc gctgcgcgca
     4681 aatcatcatt gcgcgggatg agttccggag gtggccgcac gcgagcatca tgctcgatga
     4741 gattaaatat ctgctgtcga agtacgtgct gaacgcgctg gggacgctgc gcagccggga
     4801 ctgcaacctg ctgctcgcgc accagtcgct gggcgacttc ggtcagtgcg ggcaggatct
     4861 gcccgctgat tttgtgaaaa ccacggtact ggataacacg ccgatccgct ggttctatcg
     4921 cgcggccagc caggagtccg cgcagtgggc ggcgggccag accggggaga tccgggtgga
     4981 cgtggagcgc cgccgggcca gccgggaggc ggggaacgtg gagcacatca gcggcgacag
     5041 cttcatccag aaggaggcgc ggccgctgtt tgacgtgaac accctgcaac acctgcctga
     5101 cgggttcgcg gtgatgaccg ggctgggcgt ggcgcggctg gcgttcagca gcccgctgcg
     5161 ggttgagcgc cgggaaattt cgctgaaaac cttcccggtg ctggcgaaaa cggacccgct
     5221 ggcggagtac ctgcctccgg ccgacggcgg cggaccaaaa gcggatgatg attttgcggg
     5281 gctttactga tgacactgga gcagtacaac gtcagccacg cgaagcggca ggcgcggaac
     5341 gcggagaaaa cacggctgac cctgcgctgg ctgcgggaag agctgtgttc cacggcggag
     5401 ctggttgcgc gacggctggg gattgcggcg gtacagccgg tttaccgctt cctggacagt
     5461 ctggtggcga aagggctgct ggtccgggcg aaatacccgg ttgacggccg tcaggtcagc
     5521 gtgtgggggc tgacgccgca cggggtggcg ttctcgttcg acgaggacga gccgctgacg
     5581 gacgttattc cgtttcagcc gtcaagggtg tcggcggcgc agctgccgca ccggctggcc
     5641 gtgcagtccc tgcgcctctc catggagggc cggggggcca ccggctggcg gtacctgcac
     5701 cgtatggccc tgaaagggat gaaagtgccg gacgcgctgg cggagctgga cggcagaacg
     5761 gtggcatttg aggtagagcg caccgtcaag agccgcaggc ggtatcagga agtggtgtcc
     5821 gggtatctgt tcaaccgccg ggcgaacggc atcgatgaaa tctggtacat ctgccctgac
     5881 cggccgacgc ttgtccgggt acagcgggcg attctggcgg tggatgaaat cgtgaatcca
     5941 cagaccggcg aggcccgcaa aacggtggag ctggaccggg agaggctgtt cgcgtgcttt
     6001 aaatttatga caacggagtg agggattatg gcgctgtaca tggtatttat tgaggacgtg
     6061 gaagacaaag acggcgagcg ctgcattatg ccaaccagcg agcatctgga agacatggtg
     6121 gtgctcgcct ggcagaccgg ggcgtatgtt ttctgttttg tcgaccgggc ccagcatctg
     6181 gcgatgatga aatccatcgg gatgtatgac gagcggctca acagcgacga aaaagccacg
     6241 tactttttca ccggtgaaaa tccgcaatgg gaacgggaca aacccacagg aatgaagctg
     6301 tgtgattacc tctatcgaaa ggtggagcgg ttcgccatcg agaacggtgt gcaggtggtt
     6361 gatgccggga agtgctcacc ggagcagatt aacgcgatga ttgaggtggt cagcgagcgg
     6421 cacggggaaa gcgcggtgaa cgagaagctg aaagcggagc tgatgcggga atataacagc
     6481 cgctatttta acgtgccggt gcctgaactg ccggaggaca aacccgcccg ccggtggtgg
     6541 ccgttcggca aaaaataacg ccagcaccgg catggccggt gctgttattc ttccccgagc
     6601 ttatcccgca tcacctgcca gagtgtttcc gcatcctcgt gaagccgttc gcacatgtcg
     6661 gccggggtat ccatgtccag ctcgttcagc ttttccagca acagcagcga ctgatcctta
     6721 atgaatttag ccatattgag ggcagttttt tcctgcttcg tcatgttctc cattcccttc
     6781 ggtaagttct cagctaaacc agccggacca gaagcgtttt ttcttctgtg tcagttctgt
     6841 tgtcaatctg tccacttcgg cctggagttt ccggcgctca tcatcgtgct ggcggcgttc
     6901 ttcttcccgg gatgttttat cttccagcat cagggtgaca gcctgccgga gatcggccag
     6961 ttccttttgc attgccagaa gctgttcctg tgggaaattt ttttgtgtat tttcacggtg
     7021 tgaaatgccg ggaatttgtt tctcagggat attcacattt ccgtatacac ggatcagttc
     7081 tgacacatcc acgacagggt tgtttttccc gtcacgtgac acggtaacct ttccctgttt
     7141 tacatgattg tatagcgttc ttctggtaat gcccgcggct tgtgctgcct gtgaaaggtt
     7201 gagcaatgtt ttcgccactg tatacccctg attttatcgg gtgtgaaagg tgtgtaattt
     7261 ctcacggtaa atgtatcgct gaattttacc cgtttttccg ttcaggatgt ggttttctga
     7321 cgcgctgcgc ttgtcaaacc ggcgacggcc acgcaatgaa ttgcgccccc gtcatcggtt
     7381 attagaggct gtctgccccc ggatacccgg attctttctg atgcctgacg gtgacgatca
     7441 caatctggtc catttcccgg tcgtggcggt agagcatcac atagccactg tcgccaaacc
     7501 cgatcaccag ttccttatat tccagcggca gaaacggcac cgggcggcca atgtctggca
     7561 atgttttcag ttgctggata gcccggacga tcacctcacc tccttttctg gctgccagcc
     7621 gatttttggt tttgagaaag tcctgaaggc gttgtaaatc gcgctgtgcc agcgcggaaa
     7681 tcgttacctg tggcatggcg gagcatcctg ctcgttctca gttccccagg tacttatcca
     7741 ggcttccgct tcttccgcag taaggtgcag acccgtttcc tgatagtgct gccatgcggc
     7801 ctgtccgtcc cgcaggtact ggctgcgctt ctcttcccgg tcgatgtatt cggtgattgc
     7861 ctccagcatt agcgcgtgcg ctgagcggtg ccggtcatcg gccagcgttt tcagccggtc
     7921 cttaagttct tcatcgagcc tgatggatgt tgctgatgac ataaaagccc ctttgtagtc
     7981 atatgtatta cacatgacta ctttaatccg tggcggcgct atttgccaga aatacggctt
     8041 ttgctgccca tttttcagct tcgcttccac taatcagggc tgctacaggc tcactcatca
     8101 tccaggacta ttgcagcctg cgggttaaag gacctgttac tggcggcaac catcatcatt
     8161 gagcttttgg gtggtgactg gtcgcggatc ctgtaagccg tcatttttca gactgaattc
     8221 aaaatttata acctgtcgca ttgccgggtg ccctgcggga tatagccagt gcctcattgc
     8281 cgtcagcgtt tcccgaaaaa aaaggtgaca gccggtaagc gacacgttgt ccactcgtcc
     8341 atcgtccaga atatcaaacg tggcggtcac ctgaccttcg atgcgttttg ctgcggcctg
     8401 taccgggtag gttggctgca gtgtggttac gggttttagt ggtatcgcat gtaccggagt
     8461 gaggaacatg gccgccagca ctgtcagagt aaaagctttc atggtctgaa atccagaggg
     8521 gcgtttttga atatttgcca ggtattttca tccggtgccg gcggaatttt ggcgtgggta
     8581 acggctgata ttgctgcttt acagagtttc ggatctcctc cttcagcaat ggcgctgttc
     8641 agagtgccat cacgttgtat ggatatccgc acggtgcaca tttttcccgc ataggcctct
     8701 ggttcaaaca tctgcatttc aacagcggtc ttgatggccg cagcgtagct actgatttta
     8761 gatgacttat ccagagtata aattttcggc ggctgggtgg tggaatgctt acaggccgtc
     8821 aggagtaagg ggctgcacaa tgccagcagg ataaagggta ttaaacgcat cgggttgatg
     8881 acctgtcagt aaaaaatgtt acatgtatat ttgtcactct ttacggccac gttcatttac
     8941 ataaacacgg cttatcgtat cgtttttaca ccaaacggcc cgccatgcag ttgcgtgcat
     9001 aggcatgcag cgggttacaa tttaccgggc gcgtttccgg cggttttccg ggtggtttgt
     9061 tgcctgtttt atcccgtagc cgccggaaac gccctcagac cgtctgagcg gtgcgggcaa
     9121 tgcgacgtta tggtaaatac cccttaccgg cggcgctgtg gctgccaggt gaaggtggtt
     9181 tccgtagcct cgtatttctc ccgacgcatt tcctgcgccc gcctggcttc acgttcgtcc
     9241 tgaatggcac gaagcaccct ttcccgcacc tgttgtttta ccgcctttgg cagcgttttg
     9301 ttccctttca ggcccatggc cagtgcctga gcgacaaagg cgtccgccag gcgcttttct
     9361 tcagcgggag tcagtttcag gtgcgtcata gtggcctccg ggcgtctcag gtttcccgaa
     9421 cagtttaacc gcaggtcctc tcctcatgcc agttagcttt cagatgtgtc tataccatct
     9481 gaaatgtaag tgtgatgaat gtcttaaata ataaaatttg ttgccatgag aaattttcgg
     9541 gaaaagacgc gatctgctta caggtggctc cgggcgcttt tttgtgcctg aagatgagaa
     9601 ctaatctcat atattgacac ctgaaaacgg gaggagtaag gtaataatta tactgtgtat
     9661 atgtacagta tgttggcttt aatttattgt tttaaatgtc aaagaggaaa acggtatgag
     9721 tggtggagac ggtcgaggtc cgggtaattc aggtctggga cataatggtg gtcaggccag
     9781 tgggaatgtg aacggtacgt ctggtaaagg tggcccttca tcaggtggtg gtacggatcc
     9841 aaacagcggg ccgggctggg gtacgacgca tacgccgaat ggagatattc ataactataa
     9901 tccgggggag tttggtaacg gcgggagtaa acccggtggt aatggtggta acagcggcaa
     9961 tcatagcggt agctctggtg gcggacagtc ttcggccact gcgatggcct tcggtctgcc
    10021 tgccctggct actccggggg ctgaaggact ggctttatca
//
