LOCUS       NZ_CP033392            11904 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae strain 015625 plasmid p2_015625, complete
            sequence.
ACCESSION   NZ_CP033392
VERSION     NZ_CP033392.2
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN08932863
            Assembly: GCF_003261955.3
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 11904)
  AUTHORS   Lin,J., Feng,Y. and Zong,Z.
  TITLE     The complete genome of Klebsiella pneumoniae strain WCHKP015625
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11904)
  AUTHORS   Lin,J., Long,H., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2021) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
  REMARK    Sequence update by submitter
REFERENCE   3  (bases 1 to 11904)
  AUTHORS   Lin,J., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-OCT-2018) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
REFERENCE   4  (bases 1 to 11904)
  AUTHORS   Lin,J., Liu,Y., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAY-2018) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 01:51:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,567
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,313
            CDSs (with protein)               :: 5,313
            Genes (RNA)                       :: 117
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 46 of 137
            Pseudo Genes (incomplete)         :: 88 of 137
            Pseudo Genes (internal stop)      :: 15 of 137
            Pseudo Genes (multiple problems)  :: 12 of 137
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP033392.2.
            On Aug 18, 2021 this sequence version replaced NZ_CP033392.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11904
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="015625"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p2_015625"
                     /geo_loc_name="China: Chengdu, Sichuan"
                     /collection_date="2017-07"
     gene            complement(join(10649..11904,1..232))
                     /gene="ltrA"
                     /locus_tag="DCD52_RS00005"
                     /old_locus_tag="DCD52_28820"
     CDS             complement(join(10649..11904,1..232))
                     /gene="ltrA"
                     /locus_tag="DCD52_RS00005"
                     /old_locus_tag="DCD52_28820"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226014.1"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="WP_004178082.1"
                     /translation="MMYGEEKSDSLIVAAKQANNPKGAESVERRSGAKGNAEQPHMRRT
                     QSRESMSQRLSRVREAAKQRKKERFTALFHLLTVEALEAAFLSLSRKAAAGVDGIRWMD
                     YAGNMKNNITDLHRRLHQGSYRAQPGRRHYIPKADGKQRPLGIASLEDKIVQYALVKIL
                     NAVYENDFMGFSYGFRPGRSQHDALDALATGLVRTNVNWVLDADISQFFDRVSHEWLIR
                     FTEHRIGDRRVIRLIRKWLTAGTSEEGQWRATEEGTPQGAVISPLLANIYLHYVFDLWA
                     HQWRRRYATGNVVMVRYADDIVIGFDKRYDARRFRIAMQRRLREFGLTVHPEKTRLMEF
                     GRFAAENRAIRGKGKPETFNFLGFTHISGKDRNGRFMLIRKTRRDRMTATLKAIKDGLR
                     RRWHYSIPEQGKWLRRVVQGYLNYHSVPGNFPTMQKFRTHVTNLWRRALRRRSQKDDTT
                     WTKANKLAAAWLPRVRVLHPWPVERFTARHPRQEPGA"
                     /name="ltrA"
     gene            877..1122
                     /locus_tag="DCD52_RS00010"
                     /old_locus_tag="DCD52_00075"
     CDS             877..1122
                     /locus_tag="DCD52_RS00010"
                     /old_locus_tag="DCD52_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            1396..1767
                     /gene="tolA"
                     /locus_tag="DCD52_RS00015"
                     /old_locus_tag="DCD52_00070"
     CDS             1396..1767
                     /gene="tolA"
                     /locus_tag="DCD52_RS00015"
                     /old_locus_tag="DCD52_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            1764..2129
                     /locus_tag="DCD52_RS27800"
     CDS             1764..2129
                     /locus_tag="DCD52_RS27800"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            2304..2582
                     /locus_tag="DCD52_RS00030"
                     /old_locus_tag="DCD52_00060"
     CDS             2304..2582
                     /locus_tag="DCD52_RS00030"
                     /old_locus_tag="DCD52_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            2570..2884
                     /locus_tag="DCD52_RS00045"
                     /old_locus_tag="DCD52_00055"
     CDS             2570..2884
                     /locus_tag="DCD52_RS00045"
                     /old_locus_tag="DCD52_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            3048..3476
                     /locus_tag="DCD52_RS00055"
                     /old_locus_tag="DCD52_00050"
     CDS             3048..3476
                     /locus_tag="DCD52_RS00055"
                     /old_locus_tag="DCD52_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            3502..3681
                     /locus_tag="DCD52_RS00060"
                     /old_locus_tag="DCD52_00045"
     CDS             3502..3681
                     /locus_tag="DCD52_RS00060"
                     /old_locus_tag="DCD52_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(3708..4238)
                     /locus_tag="DCD52_RS00065"
                     /old_locus_tag="DCD52_00040"
     CDS             complement(3708..4238)
                     /locus_tag="DCD52_RS00065"
                     /old_locus_tag="DCD52_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(4245..4976)
                     /gene="mobC"
                     /locus_tag="DCD52_RS00070"
                     /old_locus_tag="DCD52_00035"
     CDS             complement(4245..4976)
                     /gene="mobC"
                     /locus_tag="DCD52_RS00070"
                     /old_locus_tag="DCD52_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            complement(4976..6940)
                     /locus_tag="DCD52_RS00085"
                     /old_locus_tag="DCD52_00030"
     CDS             complement(4976..6940)
                     /locus_tag="DCD52_RS00085"
                     /old_locus_tag="DCD52_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(8424..8573)
                     /locus_tag="DCD52_RS00090"
                     /old_locus_tag="DCD52_00020"
     CDS             complement(8424..8573)
                     /locus_tag="DCD52_RS00090"
                     /old_locus_tag="DCD52_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(8642..9339)
                     /locus_tag="DCD52_RS00095"
                     /old_locus_tag="DCD52_00015"
     CDS             complement(join(8642..9090,9090..9339))
                     /locus_tag="DCD52_RS00095"
                     /old_locus_tag="DCD52_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558267.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="WP_223174630.1"
                     /translation="MASVSVCCPSCSATEGVMRNGKSTAGHQRYLCSHCRKTWQLTFTY
                     AASQPGTHQKIIDMAMNGVGCRATARLMGVGLNTILRHFKKLRPQSVTSRIQPGSDVIV
                     CAEMDEQWGYVGAKSRQRWLFYAYDRIRRAVVAHVFGERTMATLERLLSLLSVFDVVVW
                     MTDGWPMYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVI
                     GHYLNIKHYQ"
                     /name="IS1 family transposase"
     gene            complement(<9396..10544)
                     /locus_tag="DCD52_RS00100"
                     /old_locus_tag="DCD52_00010"
                     /pseudo=""
     CDS             complement(<9396..10544)
                     /locus_tag="DCD52_RS00100"
                     /old_locus_tag="DCD52_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012966.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     BGC             4390..11904
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          7743..8321
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(4249..4976)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.35e-140"
ORIGIN
        1 tcagcaggtg gaacaatgct gtaaaccgtt ctttcttccg ctgcttcgca gcttcccgca
       61 cgcgtgacag cctctgtgac atgctttccc ggctctgtgt ccggcgcatg tgtggctgtt
      121 ccgcgttccc cttggccccg ctccttcgct ccactgactc cgctcctttc gggttgttcg
      181 cctgcttcgc cgctactatg agcgagtccg acttctcctc tccgtacatc accggctatg
      241 actcctcgtc ttcccggtgc gggccatctc cgacactggc agatggtcag aggggagatc
      301 tcccggttcc cgcgtagaga tcgtattgac atgccagggt ctcagacccc gccgggtcca
      361 tgtggcactc gcagtatcgc accctatgat gttgccttcc gttaacagtg caacgtcggc
      421 acccggtaat ttaatataca tttcgtggct caatggctgg cctgtcaaca cccctgtcaa
      481 cgcttcgccc catacctcgc ggtatgcaac gcatgactcg gggaccttgt ggattgctgg
      541 tccttcaatg gtcggggact ttcacccctt gatctctaac cggtctcccg gcgcacactg
      601 tacatataca cagtataatt attaccttac tcctcccgtt ttcaggtgtc aatatatgag
      661 attagttctc atcttcaggc acaaaaaagc gcccggagcc acctgtaagc agatcgcgtc
      721 ttttcccgaa aatttctcat ggcaacaaat tttattattt aagacattca tcacacttac
      781 atttcagatg gtatagacac atctgaaagc taactggcat gaggagagga cctgcggtta
      841 aactgttcgg gaaacctgag acgcccggag gccactatga cgcacctgaa actgactccc
      901 gctgaagaaa agcgcctggc ggacgccttt gtcgctcagg cactggccat gggcctgaaa
      961 gggaacaaaa cgctgccaaa ggcggtaaaa caacaggtgc gggaaagggt gcttcgtgcc
     1021 attcaggacg aacgtgaagc caggcgggcg caggaaatgc gtcgggagaa atacgaggct
     1081 acggaaacca ccttcacctg gcagccacag cgccgccggt aaggggtatt taccataacg
     1141 tcgcattgcc cgcaccgctc agacggtctg agggcgtttc cggcggctac gggataaaac
     1201 aggcaacaaa ccacccggaa aaccgccgga aacgcgcccg gtaaattgta acccgctgca
     1261 tgcctatgca cgcaactgca tggcgggccg tttggtgtaa aaacgatacg ataagccgtg
     1321 tttatgtaaa tgaacgtggc cgtaaagagt gacaaatata catgtaacat tttttactga
     1381 caggtcatca acccgatgcg tttaataccc tttatcctgc tggcattgtg cagcccctta
     1441 ctcctgacgg cctgtaagca ttccaccacc cagccgccga aaatttatac tctggataag
     1501 tcatctaaaa tcagtagcta cgctgcggcc atcaagaccg ctgttgaaat gcagatgttt
     1561 gaaccagagg cctatgcggg aaaaatgtgc accgtgcgga tatccataca acgtgatggc
     1621 actctgaaca gcgccattgc tgaaggagga gatccgaaac tctgtaaagc agcaatatca
     1681 gccgttaccc acgccaaaat tccgccggca ccggatgaaa atacctggca aatattcaaa
     1741 aacgcccctc tggatttcag accatgaaag cttttactct gacagtgctg gcggccatgt
     1801 tcctcactcc ggtacatgcg ataccactaa aacccgtaac cacactgcag ccaacctacc
     1861 cggtacaggc cgcagcaaaa cgcatcgaag gtcaggtgac cgccacgttt gatattctgg
     1921 acgatggacg agtggacaac gtgtcgctta ccggctgtca cctttttttt cgggaaacgc
     1981 tgacggcaat gaggcactgg ctatatcccg cagggcaccc ggcaatgcga caggttataa
     2041 attttgaatt cagtctgaaa aatgacggct tacaggatcc gcgaccagtc accacccaaa
     2101 agctcaatga tgatggttgc cgccagtaac aggtccttta acccgcaggc tgcaatagtc
     2161 ctggatgatg agtgagcctg tagcagccct gattagtgga agcgaagctg aaaaatgggc
     2221 agcaaaagcc gtatttctgg caaatagcgc cgccacggat taaagtagtc atgtgtaata
     2281 catatgacta caaaggggct tttatgtcat cagcaacatc catcaggctc gatgaagaac
     2341 ttaaggaccg gctgaaaacg ctggccgatg accggcaccg ctcagcgcac gcgctaatgc
     2401 tggaggcaat caccgaatac atcgaccggg aagagaagcg cagccagtac ctgcgggacg
     2461 gacaggccgc atggcagcac tatcaggaaa cgggtctgca ccttactgcg gaagaagcgg
     2521 aagcctggat aagtacctgg ggaactgaga acgagcagga tgctccgcca tgccacaggt
     2581 aacgatttcc gcgctggcac agcgcgattt acaacgcctt caggactttc tcaaaaccaa
     2641 aaatcggctg gcagccagaa aaggaggtga ggtgatcgtc cgggctatcc agcaactgaa
     2701 aacattgcca gacattggcc gcccggtgcc gtttctgccg ctggaatata aggaactggt
     2761 gatcgggttt ggcgacagtg gctatgtgat gctctaccgc cacgaccggg aaatggacca
     2821 gattgtgatc gtcaccgtca ggcatcagaa agaatccggg tatccggggg cagacagcct
     2881 ctaataaccg atgacggggg cgcaattcat tgcgtggccg tcgccggttt gacaagcgca
     2941 gcgcgtcaga aaaccacatc ctgaacggaa aaacgggtaa aattcagcga tacatttacc
     3001 gtgagaaatt acacaccttt cacacccgat aaaatcaggg gtatacagtg gcgaaaacat
     3061 tgctcaacct ttcacaggca gcacaagccg cgggcattac cagaagaacg ctatacaatc
     3121 atgtaaaaca gggaaaggtt accgtgtcac gtgacgggaa aaacaaccct gtcgtggatg
     3181 tgtcagaact gatccgtgta tacggaaatg tgaatatccc tgagaaacaa attcccggca
     3241 tttcacaccg tgaaaataca caaaaaaatt tcccacagga acagcttctg gcaatgcaaa
     3301 aggaactggc cgatctccgg caggctgtca ccctgatgct ggaagataaa acatcccggg
     3361 aagaagaacg ccgccagcac gatgatgagc gccggaaact ccaggccgaa gtggacagat
     3421 tgacaacaga actgacacag aagaaaaaac gcttctggtc cggctggttt agctgagaac
     3481 ttaccgaagg gaatggagaa catgacgaag caggaaaaaa ctgccctcaa tatggctaaa
     3541 ttcattaagg atcagtcgct gctgttgctg gaaaagctga acgagctgga catggatacc
     3601 ccggccgaca tgtgcgaacg gcttcacgag gatgcggaaa cactctggca ggtgatgcgg
     3661 gataagctcg gggaagaata acagcaccgg ccatgccggt gctggcgtta ttttttgccg
     3721 aacggccacc accggcgggc gggtttgtcc tccggcagtt caggcaccgg cacgttaaaa
     3781 tagcggctgt tatattcccg catcagctcc gctttcagct tctcgttcac cgcgctttcc
     3841 ccgtgccgct cgctgaccac ctcaatcatc gcgttaatct gctccggtga gcacttcccg
     3901 gcatcaacca cctgcacacc gttctcgatg gcgaaccgct ccacctttcg atagaggtaa
     3961 tcacacagct tcattcctgt gggtttgtcc cgttcccatt gcggattttc accggtgaaa
     4021 aagtacgtgg ctttttcgtc gctgttgagc cgctcgtcat acatcccgat ggatttcatc
     4081 atcgccagat gctgggcccg gtcgacaaaa cagaaaacat acgccccggt ctgccaggcg
     4141 agcaccacca tgtcttccag atgctcgctg gttggcataa tgcagcgctc gccgtctttg
     4201 tcttccacgt cctcaataaa taccatgtac agcgccataa tccctcactc cgttgtcata
     4261 aatttaaagc acgcgaacag cctctcccgg tccagctcca ccgttttgcg ggcctcgccg
     4321 gtctgtggat tcacgatttc atccaccgcc agaatcgccc gctgtacccg gacaagcgtc
     4381 ggccggtcag ggcagatgta ccagatttca tcgatgccgt tcgcccggcg gttgaacaga
     4441 tacccggaca ccacttcctg ataccgcctg cggctcttga cggtgcgctc tacctcaaat
     4501 gccaccgttc tgccgtccag ctccgccagc gcgtccggca ctttcatccc tttcagggcc
     4561 atacggtgca ggtaccgcca gccggtggcc ccccggccct ccatggagag gcgcagggac
     4621 tgcacggcca gccggtgcgg cagctgcgcc gccgacaccc ttgacggctg aaacggaata
     4681 acgtccgtca gcggctcgtc ctcgtcgaac gagaacgcca ccccgtgcgg cgtcagcccc
     4741 cacacgctga cctgacggcc gtcaaccggg tatttcgccc ggaccagcag ccctttcgcc
     4801 accagactgt ccaggaagcg gtaaaccggc tgtaccgccg caatccccag ccgtcgcgca
     4861 accagctccg ccgtggaaca cagctcttcc cgcagccagc gcagggtcag ccgtgttttc
     4921 tccgcgttcc gcgcctgccg cttcgcgtgg ctgacgttgt actgctccag tgtcatcagt
     4981 aaagccccgc aaaatcatca tccgcttttg gtccgccgcc gtcggccgga ggcaggtact
     5041 ccgccagcgg gtccgttttc gccagcaccg ggaaggtttt cagcgaaatt tcccggcgct
     5101 caacccgcag cgggctgctg aacgccagcc gcgccacgcc cagcccggtc atcaccgcga
     5161 acccgtcagg caggtgttgc agggtgttca cgtcaaacag cggccgcgcc tccttctgga
     5221 tgaagctgtc gccgctgatg tgctccacgt tccccgcctc ccggctggcc cggcggcgct
     5281 ccacgtccac ccggatctcc ccggtctggc ccgccgccca ctgcgcggac tcctggctgg
     5341 ccgcgcgata gaaccagcgg atcggcgtgt tatccagtac cgtggttttc acaaaatcag
     5401 cgggcagatc ctgcccgcac tgaccgaagt cgcccagcga ctggtgcgcg agcagcaggt
     5461 tgcagtcccg gctgcgcagc gtccccagcg cgttcagcac gtacttcgac agcagatatt
     5521 taatctcatc gagcatgatg ctcgcgtgcg gccacctccg gaactcatcc cgcgcaatga
     5581 tgatttgcgc gcagcgggca aacagcatct tctgcacccg gatcaccgcc tcatcgtcca
     5641 tcgagccgat gacgtacagg cagccgccgc cgttgattat ccccgccaca tcgataccgc
     5701 tgtccgtctg caggacgctc aggtcggcca cgttctccag cttggtgatc agccccttca
     5761 cgtccttttt cagggcctcc ggcaacgcgt acgccgccgc cagcacctgc tgaatgtttg
     5821 ccccctgcgg gaactgctcc gcaatcagct tcgccgcttt ctgctcgctg atgcggtagt
     5881 ggtccgccac gtcgccggtc tcactcaggt taaacccggc aaccagcaga ttcttcaggg
     5941 cgtactggtc gagatcgcgg aacagattca gctgcgccgg tttccccggc cgcagatcca
     6001 gcagggtgaa gtccgggcag tgcgcccgga agacgtgcgg cgcccacgcg tcccctttgg
     6061 ggtcgaagta caccacagcg tccccgaaat cgcgtacgca gcgaatgagc gcgttggtgg
     6121 ccatcgcgct cttgccgctc ccgggcaggc cgaggatctg aatattggtc ttgcgccagg
     6181 tatcacgagg gaccagcacc ggctggccct gctcatcctt acccagaaac accgcctgct
     6241 gtttccgggc ctgctccgtg gaaaaatcgg ccagcgcgtt aaccggtacc ggctccccga
     6301 aatgcaggtg gcggacgtct gtcatttctc cggtccgctg gctgctgcgc cggctctcct
     6361 gattcagttt cgcgttcacc gcctcgtcag cggccgcaaa atgaccatca agcagcagga
     6421 acgccagcag ccgcgccaga aggacgccgg cgacaagccc tccggcacac caggctatcc
     6481 cgaacaggtt agccggctgc agcaggtaat aatctgcgtt cgcatagagg tccgggcgca
     6541 ggacggtttc tccccagctc tcgataaaca cggcgacaac cagccccccc agaagggcca
     6601 gaccggcagc gccggtcagc cagtacgcgt agttccagac atgtcgccgt gccctgacca
     6661 tccgcccggg tgaatccagc gaggccagca ccgaaaatgg cacagtccgg ttattatcgc
     6721 gcttatagcg atcggcatac tcccgccgcg ccttatggct cagccccaca aaacgcattc
     6781 ggccggccca gagttcagcc agaccgaaaa acaggccgat aaccagaaaa aaaccggcca
     6841 gactgtaata ctcccggtac agccagctga cccacgggta aatctgccgg gccagctccg
     6901 ggatatgcca ctgccaggca agcgaatccg tattaaacat gcaatctctc tccttcagac
     6961 gacgggttta ccggcgtgat gcgcagcccc cgctgcacaa gccgcggaca tgccccaagg
     7021 catggcggcg gcggtcacgt ccgtggtgat tgcgttcagc tttcaccgcg gaggccagcc
     7081 tctttccgcc ccatgcggaa aaggcgcagc cccggaggaa caacgagcac gccccagcgt
     7141 gtgagaggaa aaggcggcac cccagtgcgg tcttttccgg tgttccggcc cccgtcagag
     7201 ttcgccatag cgagcggctg accacccgca aaatcaggag cagctttgct gcttcgtcag
     7261 gtgcgtccag caccgtggat ttttgtgggt ggtcaggcgt gagcctgacg ggggccgggt
     7321 gaatttctgg cgtggccggc gtccagccgg acagagccag aaagagggga aaagaccgtg
     7381 cgtcggtggc gtccagccag cgtaagcaag gtcttttccc ctgaagtcag ccacgcccca
     7441 gcggggatga cttgcatttc tccgcaagga gaaaaaagca acagagatag gttaacgtaa
     7501 aatgttcagc gaaaaaccga catggttcac ctatcctgat aattgatcgt cagggaatag
     7561 aaagacttaa tcaggggaca atatcccaca tcagcgcgtc cggtgaccgt cttgcaggtg
     7621 ccggggaaat tgcgcctcct cgctcactcg gtcgctacgc tccgggcgtg agactgcggc
     7681 gggcgctgcg gacacataca aagttaccca cagattccgt ggataagcag gggactaaca
     7741 tgtgaggcaa aacggcaggg ccgcgccggt ggcgtttttc cataggctcc gccctcctgc
     7801 cagagttcac ataaacagac gctttttcgg tgcatctgtg ggagccgtga ggctcaacca
     7861 tgaatctgac agtacgggcg aaacccgaca ggacttaaag atccccaccg tttccggtag
     7921 gtcgctccct cttgcgctct cctgttccga ccctgccgtt taccggatac ctgtccgccg
     7981 ttctcccttg cgggaagtgt ggcgctttct catagctcac acactggtat ctcggctcgg
     8041 tgtaggtcgt tcgctccaag ctgggctgta agcaagaact ccccgttcag cccgactgct
     8101 gcgccttatc cggtaactgt tcacttgagt ccaacccggg aaagcacgca aaacgccact
     8161 ggcagcagcc attggtaact gggagttcgc agaggatttg tttagctaaa cacgcggttg
     8221 ctcttgaagt gtgcgccaaa gtccggctac actggaagga cagatttggt tgctgtgctc
     8281 tgcgaaagcc agttaccacg gttaagcagt tccccaactg acttaacctt cgatcaaacc
     8341 acctccccag gtggtttttt cgtttacagg gcaaaagatt acgcgcagaa aaaaaggatc
     8401 tcaagaagat cctttgatct tttctactga accgcgatcc ccgtcagttc agaggaggac
     8461 gatggtgcca ccgttccgcc ctgaacatcc cggatataat ttgcctgaca tgcggccaga
     8521 agaaagccag caacaatcaa tataaaaaga aaaatagtct ttaccttttt catatacttt
     8581 ccctgtattt aaagactccg gcattcatcc ctgaacaccg gaagtcggta atgactccaa
     8641 tttactgata gtgttttatg ttcagatagt gcccgatgac tttgtcatgc agctccaccg
     8701 attttgagaa cgacagggac ttcctgccca gccttgccag atgctgtctc agattcaggt
     8761 tgtgtcgctc aattcgctgc gtgtaacgct tactgatgac gtgcagtttt cccttcagac
     8821 gtgactcata catcggccag ccgtccgtca tccataccac cacgtcaaaa actgacagca
     8881 ggctcagaag acgctccagt gtggccatag tgcgttcacc gaaaacgtgg gctaccaccg
     8941 ccctgcgtat cctgtcatac gcgtaaaaca gccagcgctg acgtgatttt gcaccgacgt
     9001 agccccattg ctcgtccatc tccgcacaga caatgacatc actgcccggc tgtatccggg
     9061 aggttactga ctgcggcctg agttttttaa atggcgcaaa atggtgttga ggcccacgcc
     9121 cattagtcgt gcggtggcac ggcaaccgac gccgttcata gccatatcaa tgattttctg
     9181 gtgtgtaccg ggctgagaag cggcataagt gaaggtgagc tgccatgttt tacggcagtg
     9241 agagcagaga taacgttgat gcccggcagt acttttgcca ttacgcatca cgccttcagt
     9301 agcggaacag gagggacagc agacggagac tgaagccacg ggagcacctc aaaaacacca
     9361 ttatacacta aatcagcaag ttggtagcat cacccaaaat catctttatt tgcctctgcc
     9421 tcttttacct ttttatcggc ttcctgaaca gcagcccttg caccggggag cgccttctct
     9481 gtcgatgcaa tgcggctgtt taaggtcgca atatttttat cttcggcatc cagctcagct
     9541 ttcgctttat catagtctct tttcagcccc tcttccggat gagcggcgtc ccatgcctgc
     9601 tggcgacgct tttcttcctc ctgacgctgt tttacctgtg ccggggtaag aacatctgtc
     9661 accgacacat aaaccggctt ctgtccggtc tctttcggga aacgaataac ggcctcacgg
     9721 gagttaccac cggctgtaaa accagccgga cgtgaatcac ctttttcagc aacaatgcct
     9781 tttggcgggg ctttcgctgt cggaacacct ttcggtacgc tcacctgcag agacgggaga
     9841 cccggaatcg acacactgaa tacccccgga cgttttgtcg gtttcgcatc caccacaggg
     9901 acactcattg gccgacctgc gacaaccgca atatgctgcc gctcatcctt caccacatcc
     9961 acaacccgtt gtctgacacg aaccgtcgcc tggtccagtg gtaaagtact tgccggagtc
    10021 tccgtcaccg catcggccgg taatgacgtc ataatttttg acatcatttt cggatcatct
    10081 tttgcaatct cagaaggcag cacaccgtag atcgcaatcc cccacagacc aaacttaaac
    10141 ggccccttca gggcagccag cacatcagca acggcggccg acaacgcatc gccggaaact
    10201 gataaagcca gtccttcagc ccccggagta gccagggcag gcagaccgaa ggccatcgca
    10261 gtggccgaag actgtccgcc accagagcta ccgctatgat tgccgctgtt accaccatta
    10321 ccaccgggtt tactcccgcc gttaccaaac tcccccggat tatagttatg aatatctcca
    10381 ttcggcgtat gcgtcgtacc ccagcccggc ccgctgtttg gatccgtacc accacctgat
    10441 gaagggccac ctttaccaga cgtaccgttc acattcccac tggcctgacc accattatgt
    10501 cccagacctg aattacccgg acctcgaccg tctccaccac tcataccgtt ttcctctttg
    10561 acatttaaaa caataaatta aagccaacat gtcgcggtag ggatacccgt taccggatac
    10621 cccccgcaca gatcccggcg tgcgcgattt acgcaccggg ctcctgcctc gggtgtctgg
    10681 cggtgaaccg ctccacaggc catggatgaa gaacccgaac ccttggtagc catgcggctg
    10741 ccagtttgtt tgctttcgtc caggtcgtat catccttctg gctcctgcgc ctgagcgccc
    10801 ggcgccagag gtttgttacg tgtgtcctga acttctgcat ggtggggaag ttgcccggta
    10861 ccgagtgata gttcaggtat ccctgaacca ctctcctgag ccattttccc tgttcgggga
    10921 ttgagtaatg ccagcgcctt cgcagaccgt ctttgatggc tttcagagtt gccgtcatcc
    10981 gatcccggcg ggtctttcgt atcagcatga acctgccgtt gcgatctttc ccgctgatgt
    11041 gcgtgaaccc gaggaagttg aacgtttctg gtttgccttt tcccctgatg gcacggtttt
    11101 cggcagcgaa gcggccgaac tccatcagac gggttttctc cgggtgaacc gtgagtccga
    11161 actccctcag tctgcgctgc atggctatac ggaagcgccg ggcatcgtat cgtttgtcga
    11221 acccgatgac gatgtcatcg gcgtatctga ccattaccac attgcctgtg gcatagcgac
    11281 gtcgccactg atgcgcccac agatcgaaga cgtagtggag gtatatgttt gccagcagcg
    11341 gtgagatgac cgcaccctgt ggggtgcctt cctccgttgc tcgccattga ccctcctccg
    11401 acgtcccggc tgtgagccac ttacgtatga gcctgattac cctccggtcg ccgatccgat
    11461 gctctgtgaa cctgatcagc cattcgtggc tcaccctgtc gaagaactga ctgatgtcgg
    11521 catccagtac ccagtttacg ttagtgcgta ccagccctgt ggccagtgcg tccagtgcat
    11581 cgtgctggct tcgcccgggt ctgaacccgt atgagaaccc cataaagtcg ttttcataga
    11641 ctgcgttcag gattttcacc agcgcatact ggacgatctt gtcctccagc gaggcgatgc
    11701 cgagcgggcg ttgttttcca tccgcttttg ggatgtagtg acgcctgccg ggctgcgccc
    11761 tgtagctgcc ctgatgtagc ctccggtgca gatctgttat gttgttcttc atgtttccgg
    11821 cgtagtccat ccacctgatg ccatccactc cggcggccgc tttcctgctc agggagagga
    11881 atgcggcttc cagtgcttcg actg
//
