LOCUS       NZ_CP033212             3008 bp    DNA     circular CON 18-DEC-2023
DEFINITION  Enterococcus faecium strain RBWH1 plasmid pRBWH1.6, complete
            sequence.
ACCESSION   NZ_CP033212
VERSION     NZ_CP033212.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN10273305
            Assembly: GCF_003957785.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   Bohlmann,L., De Oliveira,D.M.P., El-Deeb,I.M., Brazel,E.B.,
            Harbison-Price,N., Ong,C.Y., Rivera-Hernandez,T., Ferguson,S.A.,
            Cork,A.J., Phan,M.D., Soderholm,A.T., Davies,M.R., Nimmo,G.R.,
            Dougan,G., Schembri,M.A., Cook,G.M., McEwan,A.G., von Itzstein,M.,
            McDevitt,C.A. and Walker,M.J.
  TITLE     Chemical Synergy between Ionophore PBT2 and Zinc Reverses Antibiotic
            Resistance
  JOURNAL   mBio 9 (6), e02391-18 (2018)
   PUBMED   30538186
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3008)
  AUTHORS   Bohlmann,L., De Oliviera,D., El-Deeb,I., Brazel,E.,
            Harbison-Price,N., Ong,C.-L., River-Hernandez,T., Ferguson,S.,
            Cork,A., Phan,M.-D., Davies,M., Nimmo,G., Dougan,G., Schembri,M.,
            Cook,G., McEwan,A., von Itzstein,M., McDevitt,C. and Walker,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-OCT-2018) Department of Microbiology and Immunology,
            University of Melbourne, The Doherty Institute of Infection and
            Immunology, Melbourne, Victoria 3000, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: PacBio SMRT Pipe v. 2.3.0
            Assembly Name                     :: RBWH1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 105.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/17/2023 02:36:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,093
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,878
            CDSs (with protein)               :: 2,878
            Genes (RNA)                       :: 92
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 44 of 123
            Pseudo Genes (incomplete)         :: 77 of 123
            Pseudo Genes (internal stop)      :: 24 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP033212.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3008
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="RBWH1"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pRBWH1.6"
                     /geo_loc_name="Australia: Brisbane"
                     /collection_date="2016"
                     /collected_by="Royal Brisbane Women's Hospital"
     gene            1..285
                     /locus_tag="EBB55_RS16515"
                     /old_locus_tag="EBB55_16210"
     CDS             1..285
                     /locus_tag="EBB55_RS16515"
                     /old_locus_tag="EBB55_16210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000353844.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESDF
                     DRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
                     /name="hypothetical protein"
     gene            288..704
                     /locus_tag="EBB55_RS16215"
                     /old_locus_tag="EBB55_16215"
     CDS             288..704
                     /locus_tag="EBB55_RS16215"
                     /old_locus_tag="EBB55_16215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001232859.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSYE
                     PDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKIEA
                     KLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
                     /name="hypothetical protein"
     gene            complement(841..1561)
                     /locus_tag="EBB55_RS16220"
                     /old_locus_tag="EBB55_16220"
                     /pseudo=""
     CDS             complement(841..1561)
                     /locus_tag="EBB55_RS16220"
                     /old_locus_tag="EBB55_16220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            2223..2309
                     /locus_tag="EBB55_RS16225"
                     /old_locus_tag="EBB55_16225"
     CDS             2223..2309
                     /locus_tag="EBB55_RS16225"
                     /old_locus_tag="EBB55_16225"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_125669474.1"
                     /translation="MFTNFICSLLVGLLSALFSYWLNNRNKK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     Typing          complement(842..1561)
                     /name="rep_cluster_1763"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.861"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atggacattt tagttcggaa tattgatcca aagtttgttg caacaataga caaacgttgc
       61 caagaactca cgaataaaac aggagaaaaa tggagtcgaa ataaatatct aaaagtttta
      121 atcgaaagtg attttgaccg tcaacttacg acttacaaaa aagaccaatt cgatttattg
      181 ctcgataaat ttattgctat ccaaacagaa aatactcaaa tattaagcga gtatgtctac
      241 acaaataatc aaatcattga aatgcttatc ggaggaatga tataaccatg agaaaaactt
      301 ctgtccaaaa acatgttact ttgtacccta gtcatgttga cgttttagat tccatcatcg
      361 aaaaaaatcc agatatcaat aactactcgg aagccattcg ctttttatgt atgagttatg
      421 aacccgatac caacacgacc actaccagca atcaggcaaa actaaatgct atgggaaaag
      481 aaatttccat catgactgaa atcatcgcag aatttgctga tttatatcta aaagatacag
      541 gcgttcttat tggcacaaat tctaaagtat atcaagaagt gaaagaaaaa atcgaagcta
      601 aactaaatcc atcaaatcga aatcgaccaa agaatcatca aaaagaaacc agtcaaacag
      661 tcacttctag aaatttcgac ccaacaaatt ttctaaaacg ataaaaaaca gataggagtt
      721 attcctatct gttttttatt tagaagcccc atacaatatg ctagaatgta ctagcaatct
      781 tagtcggttg taagggactt gtctacttgc ttaatgtaag gggggcgagt tcttttttat
      841 ttattcttct aaccagttac gcagagaaat ctttggcaaa tctgacgtta ggcttgaaaa
      901 agtaaattct aatttggcga ttttattgcc cttttttgct tttatttttg tgactttaag
      961 atcattgaag tattttttta gttctcgtaa tgcaggattt aatacttttt gatcaatgtt
     1021 acccatttga tagtattctg ggatatccat taagctccta aactcttcaa tacgaactac
     1081 ataataacca gtagaacggt actgcataag cagtcggaat agagtttttg catacgtaga
     1141 acgcaacgac gtgaaagcag ataattcaaa tctactaaat tcagctgtta aaccattaat
     1201 aatatattct aggtccggat taacactgac ttcaacaaac ttttttgttt tattgatttc
     1261 aaaaccagta aacaaaacaa agtagcgaat agaatcctca tcttcagtac ggtaagtaag
     1321 attaagcata tctttataga gcttttctaa atcaccgaca aaggcttttg tagcagtcat
     1381 cttataatca cttaactctt ttaattcctc gaaagaaaat ctaacttttt tcaaaccacg
     1441 atctttcata cgagcgcata atgcaaaaaa taaatccatt ctgcttagca ttgaaatttt
     1501 tcagcggtac taaattcaat tcattttgat atttgactgt taattccgtt ttttcatcca
     1561 ttcctttctt ccccccttac aaataaataa taacctttaa caaccaactc gtaaataggt
     1621 tggttgttat tatacaaaag gatattagta ctcagataaa aagttgttag tactcagata
     1681 aaaagttgtt agtactcaga taaaaagttg ttagtactca gataaaaagt tgttagtacc
     1741 cttctgtatc cgttgggaga gtaaggctca actgccccct aaaaagggtt ctaaaaaatc
     1801 tttaaaaaat aaataaaata aacgcgcgcg cgcgagcaaa atttcccgcc aaaaaatcac
     1861 ccacttttta aaataaacag gttcgcgcat gatattaaac tgccaatcta gctccctatg
     1921 gtcgctgtaa gaaggagtga tcgctacgct ctcacatttt acttgcagca gtcgccttta
     1981 ggctccttcg ccaaaaataa ttcattaggg actcctttgt ggcgtcctga gagctcgtct
     2041 aagccgttct aagacaagat tcgataaaaa ctatgtcctt cgttctaaag cgccttacag
     2101 gcggtcaggg caagccctaa caaccccaag gcaataaaaa acttaggctt atttttgctt
     2161 ttttattgta taattgattg tcgcctatcc gacagtcttg cgactggaag gaggtgtgtt
     2221 tcgtgttcac taactttatt tgctcccttc ttgttgggct tttaagcgct ttgttcagtt
     2281 actggttgaa taaccgaaac aagaagtaaa acttaattaa ggcgactcag cccacaaaaa
     2341 aagccccaga gaatccgacc tctctgggga tttcgtgtgt ttcgtgttca cttatttgca
     2401 atcaaattat aacagaaata aatatttatt tcaaaaatat cctacaaaag tctaaaatcc
     2461 gtaattattt cttatatgca acatttacaa ataaagtcgc tctgagagct cgtatttttc
     2521 gatttaagac gttttattta gtttttggtg tatttcattg gtccaagcta aaaaactctt
     2581 agaagcgatt ttagagccat ttaaacccac ttaattatat tctccatgaa attcgctcca
     2641 tgcgtcaaaa gctgcgtctg aaattccgcc gtcttcgttc actagttgca ttaatttttc
     2701 caacgatcgt tttctttcgg gtgtcatatt tttatattcc aacaattttt cgtctgttaa
     2761 atttggttat cgattacacg tgcagcactc ccccccgaat tttaaagagc ataaggaaac
     2821 gtgtacccac tttctaaaaa aagtgggtag ataggctgtg cctatcacgt ttctcagaaa
     2881 aaaatttgat atcacttttt cgccatcgtg ggtagcttat gtatagcatt ttggcaaaaa
     2941 acagctatac aaatatagta tttgtatggt atacataaat tatacataat tgcaaaagga
     3001 gatttttt
//
